RchiOBHm_Chr3g0487511

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000677
Physical Location & Seq
Reverse (-)
34721427 .. 34723705
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45194

Sequence Viewer

Length: 1668 bp
ATGGACCAGCTGAAACCTAGTTCTGCAAACCTCTGCCCTCAAACTCCCCTGGACTTCTTAGACCGAGCAGCGATCGTCTACGGCGACTGCCCCTCCATCATCTACAACTCCACCACCACCTACACCTGGTCCCAAACCCACCGTCGATGTCTCCAGCTGGCTTCCTCCATCTCCACCCTCGTCAAGAAATCCCAGGTCGTCTCCGTGGTGTCCCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTCCCCATGTCGGGAGCAGTCCTCAACAACATCAACACCCGCCTCGACGCCCACACCGTCTCCGTGCTCCTCCAACACAGCGAATCCAAGCTCGTCTTCGTCGACTTCCTCTCCCTCAACCTCGTCCTCCAAGCCCTCTCCTTGTTCCCCTCTCACATCCCCACCCCACCTCTCGTCCTCATCAACGATCATCATCAACCCTCCTCAACCTCCAGTACCGTTGACTTCATAGCGGACACGTACGACTACATGGTGGCCAAAGGCGATCCCGGGTTCGTTTGGGTCCGGCCGGCGAGCGAGTGGGACCCAATGGTGCTGAACTACACTTCAGGGACGACGTCAGCTCCGAAAGGTGCGGTCCACTGCCACAGAGCTCTCTTCAACGTGAGTCTCGTTTCAATCATCGACTGGTCGGTTCCTAAAAATTGTGTGTACCTTTGGACCCTACCGATGTTCCATGCAAATGGGTGGTGCTTCCCGTGGGTCATGGCCGCCGTGGGGGGGACCAACATCTGCCTTCGCAAGTTCGACGGTCCTATAATCTACGATCTGATCCAGAAACACAGAGTGACTCACATGTGCGCCGCGCCTGTCGTTCTCAACATGCTGGTCAACCACCCGAGAACCGACCCGCTTCCGTACCCGGTCTGTATCTTAACCGGCGGAGCTCCGCCTCCTTCGAGCGTGCTGCTCCGGGCCGAGTCGCTCGGGTTTGTTGTGAGTCACGGGTACGGGTTGACTGAAACTGCCGGGGTGGTGGTGTCGTGTGCCTGGAAGCCGGAGTGGAATAAGTTTCCGGTGACGGAGAAGGCGAGATTGAAGGCGAGGCAAGGAGTGAGGACGGTTGGAATGACGGACGCGGATGTGGTGGATCCGGATTCGGGTATGAGCGTGAAACGAAACGGGTCGGATGTTGGAGAAATAGTCATGCGAGGCGGGTGCGTCATGTTGGGTTACCTCAAAGACCCGGAAACGACGAGCGAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGCTCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAATGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAACCTGACGTCAGGCCGAGGCCGACAGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACATCAACGACGTGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

555

Amino Acids

61.01

Weight (kDa)

6.73

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 403 2.4e-70 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 4.4e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 4 cut(s) 590, 1272, 1476, 1583
AccB1I GGYRCC 1 cut(s) 1540
AccI GTMKAC 4 cut(s) 78, 351, 1583, 1637
AccII CGCG 4 cut(s) 836, 1109, 1391, 1451
AccIII TCCGGA 1 cut(s) 1123
AclWI GGATC 5 cut(s) 509, 796, 1115, 1128, 1317
AcoI YGGCCR 4 cut(s) 504, 536, 738, 1404
AcsI RAATTY 1 cut(s) 1531
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 6 cut(s) 297, 587, 1269, 1442, 1473, 1580
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 6 cut(s) 233, 466, 491, 683, 890, 980
AfiI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
AflII CTTAAG 1 cut(s) 1348
AflIII ACRYGT 2 cut(s) 486, 825
AgsI TTSAA 4 cut(s) 631, 648, 1069, 1561
AjiI CACGTC 2 cut(s) 1324, 1650
AjnI CCWGG 4 cut(s) 48, 125, 192, 1019
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 8 cut(s) 10, 157, 340, 593, 623, 917, 1525, 1570
AluI AGCT 8 cut(s) 10, 157, 340, 593, 623, 917, 1525, 1570
Alw21I GWGCWC 3 cut(s) 318, 625, 919
Alw26I GTCTC 5 cut(s) 155, 205, 313, 644, 1433
AlwI GGATC 5 cut(s) 509, 796, 1115, 1128, 1317
Ama87I CYCGRG 3 cut(s) 518, 868, 956
Aor13HI TCCGGA 1 cut(s) 1123
AoxI GGCC 7 cut(s) 504, 536, 738, 945, 1404, 1478, 1484
ApeKI GCWGC 5 cut(s) 68, 937, 1353, 1525, 1570
ApoI RAATTY 1 cut(s) 1531
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspLEI GCGC 3 cut(s) 833, 838, 1451
AsuC2I CCSGG 7 cut(s) 519, 520, 893, 944, 1000, 1217, 1286
AsuHPI GGTGA 2 cut(s) 1060, 1405
AvaI CYCGRG 3 cut(s) 518, 868, 956
AvaII GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1653
BalI TGGCCA 1 cut(s) 506
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 1 cut(s) 1540
BanII GRGCYC 2 cut(s) 625, 919
BbsI GAAGAC 3 cut(s) 337, 1553, 1583
Bbv12I GWGCWC 3 cut(s) 318, 625, 919
BbvI GCAGC 5 cut(s) 80, 924, 1365, 1512, 1557
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 4 cut(s) 97, 728, 1391, 1429
BcgI CGANNNNNNTGC 4 cut(s) 919, 953, 1170, 1204
BciT130I CCWGG 4 cut(s) 50, 127, 194, 1021
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 7 cut(s) 519, 520, 893, 944, 1000, 1217, 1286
BcoDI GTCTC 5 cut(s) 155, 205, 313, 644, 1433
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 1513
BfrI CTTAAG 1 cut(s) 1348
BglI GCCNNNNNGGC 1 cut(s) 1412
BisI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
BlpI GCTNAGC 1 cut(s) 1416
BlsI GCNGC 9 cut(s) 70, 742, 835, 939, 1355, 1390, 1405, 1527, 1572
Bme18I GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1653
BmeT110I CYCGRG 3 cut(s) 518, 868, 956
BmgBI CACGTC 2 cut(s) 1324, 1650
BmiI GGNNCC 9 cut(s) 131, 533, 554, 555, 666, 692, 754, 1122, 1542
BpiI GAAGAC 3 cut(s) 337, 1553, 1583
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 3 cut(s) 137, 445, 1319
Bpu1102I GCTNAGC 1 cut(s) 1416
BpuMI CCSGG 7 cut(s) 519, 520, 893, 944, 1000, 1217, 1286
BsaAI YACGTR 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 6 cut(s) 297, 587, 1269, 1442, 1473, 1580
BsaWI WCCGGW 2 cut(s) 1045, 1123
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
Bse118I RCCGGY 3 cut(s) 538, 908, 1262
Bse1I ACTGG 3 cut(s) 462, 662, 1656
Bse8I GATNNNNATC 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 1123
BseBI CCWGG 4 cut(s) 50, 127, 194, 1021
BseGI GGATG 3 cut(s) 405, 1117, 1165
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
BseMII CTCAG 1 cut(s) 1601
BseNI ACTGG 3 cut(s) 462, 662, 1656
BseRI GAGGAG 2 cut(s) 308, 442
BseX3I CGGCCG 2 cut(s) 536, 1404
BseXI GCAGC 5 cut(s) 80, 924, 1365, 1512, 1557
Bsh1236I CGCG 4 cut(s) 836, 1109, 1391, 1451
Bsh1285I CGRYCG 3 cut(s) 75, 539, 1407
BshFI GGCC 7 cut(s) 506, 538, 740, 947, 1406, 1480, 1486
BshNI GGYRCC 1 cut(s) 1540
BsiEI CGRYCG 3 cut(s) 75, 539, 1407
BsiHKAI GWGCWC 3 cut(s) 318, 625, 919
BsiHKCI CYCGRG 3 cut(s) 518, 868, 956
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BslI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
BsmAI GTCTC 5 cut(s) 155, 205, 313, 644, 1433
BsmBI CGTCTC 3 cut(s) 205, 313, 1433
BsmFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BsnI GGCC 7 cut(s) 506, 538, 740, 947, 1406, 1480, 1486
BsoBI CYCGRG 3 cut(s) 518, 868, 956
Bsp1286I GDGCHC 3 cut(s) 318, 625, 919
Bsp13I TCCGGA 1 cut(s) 1123
Bsp143I GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
Bsp1720I GCTNAGC 1 cut(s) 1416
BspANI GGCC 7 cut(s) 506, 538, 740, 947, 1406, 1480, 1486
BspCNI CTCAG 1 cut(s) 1602
BspEI TCCGGA 1 cut(s) 1123
BspFNI CGCG 4 cut(s) 836, 1109, 1391, 1451
BspLI GGNNCC 9 cut(s) 131, 533, 554, 555, 666, 692, 754, 1122, 1542
BspPI GGATC 5 cut(s) 509, 796, 1115, 1128, 1317
BspT107I GGYRCC 1 cut(s) 1540
BspTI CTTAAG 1 cut(s) 1348
BsrFI RCCGGY 3 cut(s) 538, 908, 1262
BsrI ACTGG 3 cut(s) 462, 662, 1656
BssAI RCCGGY 3 cut(s) 538, 908, 1262
BssMI GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
BssNI GRCGYC 6 cut(s) 297, 587, 1269, 1442, 1473, 1580
BssT1I CCWWGG 1 cut(s) 1317
Bst2UI CCWGG 4 cut(s) 50, 127, 194, 1021
Bst4CI ACNGT 6 cut(s) 143, 307, 469, 782, 1093, 1552
Bst6I CTCTTC 1 cut(s) 632
BstACI GRCGYC 6 cut(s) 297, 587, 1269, 1442, 1473, 1580
BstAFI CTTAAG 1 cut(s) 1348
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 159, 540, 544, 935
BstDEI CTNAG 3 cut(s) 58, 1416, 1610
BstDSI CCRYGG 5 cut(s) 204, 728, 744, 1389, 1407
BstEII GGTNACC 1 cut(s) 1202
BstF5I GGATG 3 cut(s) 405, 1117, 1165
BstFNI CGCG 4 cut(s) 836, 1109, 1391, 1451
BstHHI GCGC 3 cut(s) 833, 838, 1451
BstKTI GATC 8 cut(s) 75, 439, 517, 799, 804, 1123, 1312, 1329
BstMAI GTCTC 5 cut(s) 155, 205, 313, 644, 1433
BstMBI GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
BstMCI CGRYCG 3 cut(s) 75, 539, 1407
BstMWI GCNNNNNNNGC 1 cut(s) 1412
BstNI CCWGG 4 cut(s) 50, 127, 194, 1021
BstNSI RCATGY 2 cut(s) 829, 856
BstPI GGTNACC 1 cut(s) 1202
BstSFI CTRYAG 1 cut(s) 1513
BstUI CGCG 4 cut(s) 836, 1109, 1391, 1451
BstV1I GCAGC 5 cut(s) 80, 924, 1365, 1512, 1557
BstV2I GAAGAC 3 cut(s) 337, 1553, 1583
BstX2I RGATCY 1 cut(s) 1120
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 1 cut(s) 1120
BstZI CGGCCG 2 cut(s) 536, 1404
BsuRI GGCC 7 cut(s) 506, 538, 740, 947, 1406, 1480, 1486
BtgI CCRYGG 5 cut(s) 204, 728, 744, 1389, 1407
BtrI CACGTC 2 cut(s) 1324, 1650
BtsCI GGATG 3 cut(s) 405, 1117, 1165
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 610
Cac8I GCNNGC 4 cut(s) 159, 540, 544, 935
CfoI GCGC 3 cut(s) 833, 838, 1451
Cfr10I RCCGGY 3 cut(s) 538, 908, 1262
Cfr42I CCGCGG 1 cut(s) 1392
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 4 cut(s) 305, 1115, 1180, 1450
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 6 cut(s) 232, 465, 490, 682, 889, 979
CviQI GTAC 6 cut(s) 232, 465, 490, 682, 889, 979
DdeI CTNAG 3 cut(s) 58, 1416, 1610
DpnI GATC 8 cut(s) 74, 438, 516, 798, 803, 1122, 1311, 1328
DpnII GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
DraIII CACNNNGTG 1 cut(s) 817
EaeI YGGCCR 4 cut(s) 504, 536, 738, 1404
EagI CGGCCG 2 cut(s) 536, 1404
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
EciI GGCGGA 2 cut(s) 909, 927
Ecl136II GAGCTC 2 cut(s) 623, 917
EclXI CGGCCG 2 cut(s) 536, 1404
Eco130I CCWWGG 1 cut(s) 1317
Eco24I GRGCYC 2 cut(s) 625, 919
Eco47I GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1653
Eco52I CGGCCG 2 cut(s) 536, 1404
Eco53kI GAGCTC 2 cut(s) 623, 917
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 3 cut(s) 518, 868, 956
Eco91I GGTNACC 1 cut(s) 1202
EcoICRI GAGCTC 2 cut(s) 623, 917
EcoO109I RGGNCCY 1 cut(s) 553
EcoO65I GGTNACC 1 cut(s) 1202
EcoRII CCWGG 4 cut(s) 48, 125, 192, 1019
EcoT14I CCWWGG 1 cut(s) 1317
EcoT38I GRGCYC 2 cut(s) 625, 919
ErhI CCWWGG 1 cut(s) 1317
Esp3I CGTCTC 3 cut(s) 205, 313, 1433
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
FauI CCCGC 5 cut(s) 232, 296, 888, 1178, 1328
FbaI TGATCA 1 cut(s) 1326
FblI GTMKAC 4 cut(s) 78, 351, 1583, 1637
Fnu4HI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
FokI GGATG 3 cut(s) 392, 1124, 1172
FriOI GRGCYC 2 cut(s) 625, 919
Fsp4HI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
FspBI CTAG 1 cut(s) 18
GlaI GCGC 3 cut(s) 832, 837, 1450
GluI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
GsuI CTGGAG 3 cut(s) 137, 445, 1319
HaeIII GGCC 7 cut(s) 506, 538, 740, 947, 1406, 1480, 1486
HgaI GACGC 4 cut(s) 305, 1115, 1180, 1450
HhaI GCGC 3 cut(s) 833, 838, 1451
Hin1I GRCGYC 6 cut(s) 297, 587, 1269, 1442, 1473, 1580
Hin6I GCGC 3 cut(s) 831, 836, 1449
HinP1I GCGC 3 cut(s) 831, 836, 1449
HincII GTYRAC 5 cut(s) 352, 472, 862, 987, 1584
HindII GTYRAC 5 cut(s) 352, 472, 862, 987, 1584
HinfI GANTC 8 cut(s) 332, 637, 820, 950, 970, 1127, 1367, 1594
HphI GGTGA 2 cut(s) 1060, 1405
Hpy188I TCNGA 4 cut(s) 597, 801, 1159, 1599
Hpy188III TCNNGA 5 cut(s) 184, 261, 805, 1124, 1298
HpyAV CCTTC 5 cut(s) 776, 936, 1051, 1063, 1489
HpyCH4III ACNGT 6 cut(s) 143, 307, 469, 782, 1093, 1552
HpyCH4IV ACGT 8 cut(s) 488, 587, 633, 1269, 1323, 1473, 1580, 1649
HpyCH4V TGCA 3 cut(s) 26, 710, 1282
HpyF10VI GCNNNNNNNGC 1 cut(s) 1412
HpyF3I CTNAG 3 cut(s) 58, 1416, 1610
HpySE526I ACGT 8 cut(s) 488, 587, 633, 1269, 1323, 1473, 1580, 1649
Hsp92I GRCGYC 6 cut(s) 297, 587, 1269, 1442, 1473, 1580
HspAI GCGC 3 cut(s) 831, 836, 1449
KflI GGGWCCC 1 cut(s) 553
Kpn2I TCCGGA 1 cut(s) 1123
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 1326
KspI CCGCGG 1 cut(s) 1392
Kzo9I GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
LmnI GCTCC 7 cut(s) 263, 321, 598, 914, 922, 945, 1319
Lsp1109I GCAGC 5 cut(s) 80, 924, 1365, 1512, 1557
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 8 cut(s) 488, 587, 633, 1269, 1323, 1473, 1580, 1649
MaeIII GTNAC 4 cut(s) 817, 971, 1048, 1202
MalI GATC 8 cut(s) 74, 438, 516, 798, 803, 1122, 1311, 1328
MboI GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
MboII GAAGA 5 cut(s) 337, 619, 1558, 1588, 1603
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 3 cut(s) 318, 625, 919
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 2 cut(s) 673, 1531
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 5 cut(s) 646, 814, 959, 979, 1376
MmeI TCCRAC 4 cut(s) 346, 1075, 1137, 1144
Mox20I TGGCCA 1 cut(s) 506
MroI TCCGGA 1 cut(s) 1123
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 506
MseI TTAA 2 cut(s) 905, 1349
MslI CAYNNNNRTG 1 cut(s) 711
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 3 cut(s) 10, 157, 1391
MspCI CTTAAG 1 cut(s) 1348
MteI GCGCNGCGC 1 cut(s) 834
MvaI CCWGG 4 cut(s) 50, 127, 194, 1021
MvnI CGCG 4 cut(s) 836, 1109, 1391, 1451
MwoI GCNNNNNNNGC 1 cut(s) 1412
NaeI GCCGGC 1 cut(s) 540
NciI CCSGG 7 cut(s) 519, 520, 893, 944, 1000, 1217, 1286
NdeII GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
NgoMIV GCCGGC 1 cut(s) 538
NlaIV GGNNCC 9 cut(s) 131, 533, 554, 555, 666, 692, 754, 1122, 1542
NmeAIII GCCGAG 2 cut(s) 973, 1506
NmuCI GTSAC 3 cut(s) 817, 971, 1048
NspI RCATGY 2 cut(s) 829, 856
PciI ACATGT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 8 cut(s) 81, 303, 348, 593, 639, 1058, 1404, 1448
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 332, 1127, 1594
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 2 cut(s) 586, 1651
PkrI GCNGC 9 cut(s) 70, 742, 835, 939, 1355, 1390, 1405, 1527, 1572
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 645, 814, 958, 978, 1375
PpsI GAGTC 5 cut(s) 645, 814, 958, 978, 1375
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 553
PscI ACATGT 1 cut(s) 825
Psp124BI GAGCTC 2 cut(s) 625, 919
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 4 cut(s) 48, 125, 192, 1019
PspEI GGTNACC 1 cut(s) 1202
PspGI CCWGG 4 cut(s) 48, 125, 192, 1019
PspLI CGTACG 1 cut(s) 489
PspN4I GGNNCC 9 cut(s) 131, 533, 554, 555, 666, 692, 754, 1122, 1542
PspPPI RGGWCCY 1 cut(s) 553
PsuI RGATCY 1 cut(s) 1120
PsyI GACNNNGTC 2 cut(s) 586, 1651
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 6 cut(s) 233, 466, 491, 683, 890, 980
RsaNI GTAC 6 cut(s) 232, 465, 490, 682, 889, 979
RseI CAYNNNNRTG 1 cut(s) 711
SacI GAGCTC 2 cut(s) 625, 919
SacII CCGCGG 1 cut(s) 1392
SalI GTCGAC 2 cut(s) 350, 1582
SaqAI TTAA 2 cut(s) 905, 1349
SatI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
Sau3AI GATC 8 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1326
SchI GAGTC 5 cut(s) 646, 814, 959, 979, 1376
SduI GDGCHC 3 cut(s) 318, 625, 919
SexAI ACCWGGT 1 cut(s) 125
SfcI CTRYAG 1 cut(s) 1513
Sfr303I CCGCGG 1 cut(s) 1392
SgrAI CRCCGGYG 1 cut(s) 1262
SgrBI CCGCGG 1 cut(s) 1392
SgrDI CGTCGACG 1 cut(s) 1582
SinI GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1653
SmaI CCCGGG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 1348
SmoI CTYRAG 1 cut(s) 1348
Sse9I AATT 2 cut(s) 673, 1531
SspI AATATT 1 cut(s) 1630
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 2 cut(s) 625, 919
StyI CCWWGG 1 cut(s) 1317
TaaI ACNGT 6 cut(s) 143, 307, 469, 782, 1093, 1552
TaiI ACGT 8 cut(s) 491, 590, 636, 1272, 1326, 1476, 1583, 1652
TaqI TCGA 8 cut(s) 145, 294, 351, 654, 777, 929, 1365, 1583
TaqII GACCGA 1 cut(s) 78
TasI AATT 2 cut(s) 673, 1531
TauI GCSGC 4 cut(s) 743, 836, 1391, 1406
TfiI GAWTC 3 cut(s) 332, 1127, 1594
Tru1I TTAA 2 cut(s) 905, 1349
Tru9I TTAA 2 cut(s) 905, 1349
TscAI CASTG 1 cut(s) 617
TseFI GTSAC 3 cut(s) 817, 971, 1048
TseI GCWGC 5 cut(s) 68, 937, 1353, 1525, 1570
Tsp45I GTSAC 3 cut(s) 817, 971, 1048
TspDTI ATGAA 2 cut(s) 466, 1525
TspGWI ACGGA 5 cut(s) 193, 301, 876, 1067, 1118
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 1 cut(s) 617
Tth111I GACNNNGTC 2 cut(s) 586, 1651
Vha464I CTTAAG 1 cut(s) 1348
VpaK11BI GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1653
XapI RAATTY 1 cut(s) 1531
XceI RCATGY 2 cut(s) 829, 856
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 4 cut(s) 78, 351, 1583, 1637
XspI CTAG 1 cut(s) 18
ZraI GACGTC 4 cut(s) 588, 1270, 1474, 1581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.