pycom17g27150

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000677
Physical Location & Seq
Forward (+)
25409175 .. 25410834
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27150.2

Sequence Viewer

Length: 1005 bp
ATGGAAGATCTGAAGCCCACGTCAGCAAACTCATCCCCTCTAACACCCTTGGGCTTTCTGGAAAGAGCAGCCACCGTCTACGGCGACTGCCCGTCCGTAATATACGGAGACACCACCTACACTTGGACACAGACCCACCGTCGATGTCTCCGCGTGGCTTCCTCTATATCATCCCTTGGTATCAAGACAGGCAACGTGGTGTCCGTTCTCTCCCCAAACATCCCAGCCATGTACGAGCTCCACTTCGCCGTGCCCATGTCCGGCGCCATCCTCAACAACATCAACACCCGCCTGGACAGCCGCACCGTCTCCGTACTCCTTCACGGGTATGGGCTGACCGAAACGGCGGGGCTGGTGGTGTCGTGCGTGTGGAAGAGGAAGTGGAATTTGTTGCCGGCGACTAAGAGAGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGACGGTCGGGCTGACCCGAATGGACGTGGTAAACCCGGACTCCGGACTCAGCGTAAAACGCGACGGCACGTCGCTCGGCGAAGTCGTTTTGAGAGGCGGGTGCGTGATGCTAGGGTATTTGAAAGACCAATCGGGAACGGCCAAGTGCATGAAAGACGGGTGGTTATACACGGGTGACGTGGGCGTGATGCATCCCGACGGCTATCTGGAAATTAAGGACAGATCCAAGGACGTTATTATAAGCGGGGGCGAGAATCTGAGCAGTGTGGAGGTGGAGTCGGTGCTGTATACGAACCCAGCGGTCAACGAGGCGGCGGTGGTGGCGAGGCCGGACGAGTTTTGGGGGGAGACGCCGTGTGCATTCGTGAGCTTGAAGAGTGAGGTGAGGCCGAGGCCGACGGAGAAGGAGATCATGGAGTATTGCAGGAAGAAGCTGCCGAAATACATGGTGCCAAAAACGGTGGTGTTTACGGACGAGCTTCCCAAGACATCGACCGGGAAGATTCAGAAGTTTGTGCTGAGAGAGATGGCGAAGGGTTTGTGTTCGTCGAGGCCGAGTCGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

335

Amino Acids

36.69

Weight (kDa)

9.24

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 677
AccB1I GGYRCC 2 cut(s) 263, 886
AccI GTMKAC 2 cut(s) 78, 725
AccII CGCG 2 cut(s) 153, 498
AccIII TCCGGA 1 cut(s) 479
AciI CCGC 9 cut(s) 151, 289, 301, 347, 534, 681, 737, 749, 752
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 2 cut(s) 32, 437
AcyI GRCGYC 2 cut(s) 264, 788
AfaI GTAC 2 cut(s) 233, 315
AfiI CCNNNNNNNGG 3 cut(s) 123, 260, 479
AgsI TTSAA 2 cut(s) 559, 811
AhdI GACNNNNNGTC 3 cut(s) 91, 138, 505
AjiI CACGTC 4 cut(s) 21, 463, 507, 616
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 4 cut(s) 238, 807, 871, 916
AluI AGCT 4 cut(s) 238, 807, 871, 916
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 4 cut(s) 102, 152, 313, 779
AlwI GGATC 1 cut(s) 654
Aor13HI TCCGGA 1 cut(s) 479
AoxI GGCC 5 cut(s) 576, 764, 824, 830, 989
ApeKI GCWGC 2 cut(s) 68, 871
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 266
AsuC2I CCSGG 2 cut(s) 473, 934
AsuHPI GGTGA 2 cut(s) 623, 832
BaeGI GKGCMC 1 cut(s) 255
BanI GGYRCC 2 cut(s) 263, 886
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 80, 858
BccI CCATC 2 cut(s) 275, 958
BceAI ACGGC 7 cut(s) 97, 233, 360, 517, 591, 652, 775
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 1 cut(s) 293
BcnI CCSGG 2 cut(s) 473, 934
BcoDI GTCTC 4 cut(s) 102, 152, 313, 779
BfaI CTAG 1 cut(s) 548
BfoI RGCGCY 1 cut(s) 267
BglII AGATCT 1 cut(s) 7
BisI GCNGC 4 cut(s) 69, 301, 750, 872
BlsI GCNGC 4 cut(s) 70, 302, 751, 873
Bme1390I CCNGG 3 cut(s) 293, 473, 934
BmeRI GACNNNNNGTC 3 cut(s) 91, 138, 505
BmgBI CACGTC 4 cut(s) 21, 463, 507, 616
BmiI GGNNCC 2 cut(s) 265, 888
BmrFI CCNGG 3 cut(s) 293, 473, 934
BmsI GCATC 3 cut(s) 534, 615, 637
BpuMI CCSGG 2 cut(s) 473, 934
BsaHI GRCGYC 2 cut(s) 264, 788
BsaJI CCNNGG 4 cut(s) 48, 175, 663, 827
BsaWI WCCGGW 1 cut(s) 479
BsaXI ACNNNNNCTCC 6 cut(s) 461, 491, 698, 728, 776, 806
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 260, 479
Bse118I RCCGGY 1 cut(s) 394
BseAI TCCGGA 1 cut(s) 479
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 4 cut(s) 48, 175, 663, 827
BseGI GGATG 5 cut(s) 32, 170, 219, 267, 628
BseLI CCNNNNNNNGG 3 cut(s) 123, 260, 479
BseMII CTCAG 3 cut(s) 499, 686, 947
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 2 cut(s) 80, 858
BseYI CCCAGC 2 cut(s) 223, 733
Bsh1236I CGCG 2 cut(s) 153, 498
Bsh1285I CGRYCG 2 cut(s) 444, 933
BshFI GGCC 5 cut(s) 578, 766, 826, 832, 991
BshNI GGYRCC 2 cut(s) 263, 886
BsiEI CGRYCG 2 cut(s) 444, 933
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 6 cut(s) 261, 395, 473, 480, 767, 933
BslI CCNNNNNNNGG 3 cut(s) 123, 260, 479
BsmAI GTCTC 4 cut(s) 102, 152, 313, 779
BsmBI CGTCTC 2 cut(s) 313, 779
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 5 cut(s) 578, 766, 826, 832, 991
Bsp1286I GDGCHC 2 cut(s) 240, 255
Bsp13I TCCGGA 1 cut(s) 479
Bsp143I GATC 3 cut(s) 7, 659, 846
BspACI CCGC 9 cut(s) 151, 289, 301, 347, 534, 681, 737, 749, 752
BspANI GGCC 5 cut(s) 578, 766, 826, 832, 991
BspCNI CTCAG 3 cut(s) 498, 687, 948
BspEI TCCGGA 1 cut(s) 479
BspFNI CGCG 2 cut(s) 153, 498
BspLI GGNNCC 2 cut(s) 265, 888
BspPI GGATC 1 cut(s) 654
BspT107I GGYRCC 2 cut(s) 263, 886
BsrFI RCCGGY 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 4 cut(s) 48, 175, 663, 827
BssMI GATC 3 cut(s) 7, 659, 846
BssNAI GTATAC 1 cut(s) 726
BssNI GRCGYC 2 cut(s) 264, 788
BssT1I CCWWGG 3 cut(s) 48, 175, 663
Bst1107I GTATAC 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 5 cut(s) 76, 140, 307, 442, 898
Bst6I CTCTTC 2 cut(s) 368, 806
BstACI GRCGYC 2 cut(s) 264, 788
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 4 cut(s) 402, 485, 695, 956
BstF5I GGATG 5 cut(s) 32, 170, 219, 267, 628
BstFNI CGCG 2 cut(s) 153, 498
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 3 cut(s) 10, 662, 849
BstMAI GTCTC 4 cut(s) 102, 152, 313, 779
BstMBI GATC 3 cut(s) 7, 659, 846
BstMCI CGRYCG 2 cut(s) 444, 933
BstMWI GCNNNNNNNGC 3 cut(s) 297, 495, 758
BstNI CCWGG 1 cut(s) 293
BstSCI CCNGG 3 cut(s) 291, 471, 932
BstSLI GKGCMC 1 cut(s) 255
BstUI CGCG 2 cut(s) 153, 498
BstV1I GCAGC 2 cut(s) 80, 858
BstX2I RGATCY 2 cut(s) 7, 659
BstYI RGATCY 2 cut(s) 7, 659
BstZ17I GTATAC 1 cut(s) 726
BsuRI GGCC 5 cut(s) 578, 766, 826, 832, 991
BtrI CACGTC 4 cut(s) 21, 463, 507, 616
BtsCI GGATG 5 cut(s) 32, 170, 219, 267, 628
BtsI GCAGTG 1 cut(s) 706
BtsIMutI CAGTG 1 cut(s) 706
Cac8I GCNNGC 1 cut(s) 396
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 394
CseI GACGC 1 cut(s) 796
Csp6I GTAC 2 cut(s) 232, 314
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviAII CATG 5 cut(s) 229, 256, 586, 850, 883
CviQI GTAC 2 cut(s) 232, 314
DdeI CTNAG 4 cut(s) 402, 485, 695, 956
DinI GGCGCC 1 cut(s) 265
DpnI GATC 3 cut(s) 9, 661, 848
DpnII GATC 3 cut(s) 7, 659, 846
DriI GACNNNNNGTC 3 cut(s) 91, 138, 505
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 2 cut(s) 368, 806
Eam1105I GACNNNNNGTC 3 cut(s) 91, 138, 505
EarI CTCTTC 2 cut(s) 368, 806
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 3 cut(s) 48, 175, 663
Eco24I GRGCYC 1 cut(s) 240
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 32, 437
EcoICRI GAGCTC 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 3 cut(s) 48, 175, 663
EcoT22I ATGCAT 1 cut(s) 630
EcoT38I GRGCYC 1 cut(s) 240
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 3 cut(s) 48, 175, 663
Esp3I CGTCTC 2 cut(s) 313, 779
FaeI CATG 5 cut(s) 232, 259, 589, 853, 886
FatI CATG 5 cut(s) 228, 255, 585, 849, 882
FauI CCCGC 4 cut(s) 296, 340, 527, 674
FblI GTMKAC 2 cut(s) 78, 725
Fnu4HI GCNGC 4 cut(s) 69, 301, 750, 872
FokI GGATG 5 cut(s) 19, 157, 206, 254, 615
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 4 cut(s) 69, 301, 750, 872
FspBI CTAG 1 cut(s) 548
GlaI GCGC 1 cut(s) 265
GluI GCNGC 4 cut(s) 69, 301, 750, 872
GsaI CCCAGC 2 cut(s) 227, 737
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 5 cut(s) 578, 766, 826, 832, 991
HapII CCGG 6 cut(s) 261, 395, 473, 480, 767, 933
HgaI GACGC 1 cut(s) 796
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 2 cut(s) 264, 788
Hin1II CATG 5 cut(s) 232, 259, 589, 853, 886
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HinfI GANTC 6 cut(s) 476, 483, 691, 713, 940, 994
HpaII CCGG 6 cut(s) 261, 395, 473, 480, 767, 933
HphI GGTGA 2 cut(s) 623, 832
Hpy166II GTNNAC 5 cut(s) 79, 469, 726, 742, 906
Hpy188I TCNGA 3 cut(s) 12, 696, 945
Hpy188III TCNNGA 7 cut(s) 59, 184, 480, 570, 632, 644, 802
Hpy8I GTNNAC 5 cut(s) 79, 469, 726, 742, 906
Hpy99I CGWCG 6 cut(s) 144, 503, 511, 638, 838, 988
HpyAV CCTTC 3 cut(s) 329, 835, 964
HpyCH4III ACNGT 5 cut(s) 76, 140, 307, 442, 898
HpyCH4IV ACGT 6 cut(s) 20, 195, 462, 506, 615, 669
HpyCH4V TGCA 4 cut(s) 585, 628, 797, 861
HpyF10VI GCNNNNNNNGC 3 cut(s) 297, 495, 758
HpyF3I CTNAG 4 cut(s) 402, 485, 695, 956
HpySE526I ACGT 6 cut(s) 20, 195, 462, 506, 615, 669
Hsp92I GRCGYC 2 cut(s) 264, 788
Hsp92II CATG 5 cut(s) 232, 259, 589, 853, 886
HspAI GCGC 1 cut(s) 264
KasI GGCGCC 1 cut(s) 263
Kpn2I TCCGGA 1 cut(s) 479
KroI GCCGGC 1 cut(s) 394
KroNI GCCGGC 1 cut(s) 396
Kzo9I GATC 3 cut(s) 7, 659, 846
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 2 cut(s) 80, 858
LweI GCATC 3 cut(s) 534, 615, 637
MaeI CTAG 1 cut(s) 548
MaeII ACGT 6 cut(s) 20, 195, 462, 506, 615, 669
MaeIII GTNAC 1 cut(s) 611
MalI GATC 3 cut(s) 9, 661, 848
MboI GATC 3 cut(s) 7, 659, 846
MboII GAAGA 5 cut(s) 17, 385, 823, 877, 949
MflI RGATCY 2 cut(s) 7, 659
MhlI GDGCHC 2 cut(s) 240, 255
MluCI AATT 2 cut(s) 385, 648
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 4 cut(s) 470, 477, 722, 1003
Mph1103I ATGCAT 1 cut(s) 630
MroI TCCGGA 1 cut(s) 479
MroNI GCCGGC 1 cut(s) 394
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 6 cut(s) 261, 395, 473, 480, 767, 933
MspR9I CCNGG 3 cut(s) 293, 473, 934
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 2 cut(s) 153, 498
MwoI GCNNNNNNNGC 3 cut(s) 297, 495, 758
NaeI GCCGGC 1 cut(s) 396
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 473, 934
NdeII GATC 3 cut(s) 7, 659, 846
NgoMIV GCCGGC 1 cut(s) 394
NlaIII CATG 5 cut(s) 232, 259, 589, 853, 886
NlaIV GGNNCC 2 cut(s) 265, 888
NmeAIII GCCGAG 2 cut(s) 492, 852
NmuCI GTSAC 1 cut(s) 611
NsiI ATGCAT 1 cut(s) 630
PcsI WCGNNNNNNNCGW 3 cut(s) 201, 519, 989
PctI GAATGC 1 cut(s) 797
PdiI GCCGGC 1 cut(s) 396
PfeI GAWTC 2 cut(s) 691, 940
PkrI GCNGC 4 cut(s) 70, 302, 751, 873
PleI GAGTC 4 cut(s) 470, 477, 721, 1002
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 4 cut(s) 470, 477, 721, 1002
PsiI TTATAA 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 291
PspFI CCCAGC 2 cut(s) 223, 733
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 265, 888
PsuI RGATCY 2 cut(s) 7, 659
RsaI GTAC 2 cut(s) 233, 315
RsaNI GTAC 2 cut(s) 232, 314
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 4 cut(s) 69, 301, 750, 872
Sau3AI GATC 3 cut(s) 7, 659, 846
SchI GAGTC 4 cut(s) 470, 477, 722, 1003
ScrFI CCNGG 3 cut(s) 293, 473, 934
SduI GDGCHC 2 cut(s) 240, 255
SfaNI GCATC 3 cut(s) 534, 615, 637
SfoI GGCGCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 327
Sse9I AATT 2 cut(s) 385, 648
SsiI CCGC 9 cut(s) 151, 289, 301, 347, 534, 681, 737, 749, 752
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 548
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 3 cut(s) 291, 471, 932
StyI CCWWGG 3 cut(s) 48, 175, 663
TaaI ACNGT 5 cut(s) 76, 140, 307, 442, 898
TaiI ACGT 6 cut(s) 23, 198, 465, 509, 618, 672
TaqI TCGA 5 cut(s) 142, 422, 929, 986, 997
TaqII GACCGA 1 cut(s) 353
TasI AATT 2 cut(s) 385, 648
TauI GCSGC 2 cut(s) 303, 752
TfiI GAWTC 2 cut(s) 691, 940
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 2 cut(s) 68, 871
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 1 cut(s) 602
TspGWI ACGGA 6 cut(s) 85, 120, 193, 301, 851, 923
TspRI CASTG 1 cut(s) 706
XapI RAATTY 1 cut(s) 385
XmiI GTMKAC 2 cut(s) 78, 725
XspI CTAG 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.