Rh3BG317800

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000677
Physical Location & Seq
Forward (+)
32542314 .. 32542805
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG317800.1

Sequence Viewer

Length: 492 bp
ATGCGAGGCGGGTGCGTCATGTTGGGCTACCTCAAAGACCCAGAAACGACGAGCAATACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCCGCCGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTCAGGCCGAGGCCGACCGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTCTATACTGAGAGAGATTGCGAAAAATATTGGGTCTACATCAACGACGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

163

Amino Acids

17.98

Weight (kDa)

7.66

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 62 - 139 6.9e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 96, 300, 407
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 2 cut(s) 407, 461
AccII CGCG 1 cut(s) 275
AciI CCGC 4 cut(s) 9, 159, 213, 227
AclWI GGATC 2 cut(s) 132, 141
AcoI YGGCCR 1 cut(s) 228
AcsI RAATTY 1 cut(s) 355
AcyI GRCGYC 4 cut(s) 93, 266, 297, 404
AgsI TTSAA 1 cut(s) 385
AjiI CACGTC 1 cut(s) 148
AluBI AGCT 3 cut(s) 293, 349, 394
AluI AGCT 3 cut(s) 293, 349, 394
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 2 cut(s) 132, 141
AoxI GGCC 3 cut(s) 228, 302, 308
ApeKI GCWGC 3 cut(s) 177, 349, 394
ApoI RAATTY 1 cut(s) 355
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 477
AsuC2I CCSGG 1 cut(s) 110
AvaII GGWCC 1 cut(s) 477
BanI GGYRCC 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 377, 407
BbvI GCAGC 3 cut(s) 189, 336, 381
BceAI ACGGC 3 cut(s) 200, 215, 253
BcgI CGANNNNNNTGC 1 cut(s) 28
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 257
BfmI CTRYAG 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 236
BisI GCNGC 5 cut(s) 178, 213, 228, 350, 395
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 5 cut(s) 179, 214, 229, 351, 396
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 477
BmgBI CACGTC 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 477
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 1 cut(s) 110
BpiI GAAGAC 2 cut(s) 377, 407
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 110
Bsa29I ATCGAT 1 cut(s) 136
BsaHI GRCGYC 4 cut(s) 93, 266, 297, 404
BsaJI CCNNGG 3 cut(s) 141, 231, 305
BsaXI ACNNNNNCTCC 4 cut(s) 176, 206, 254, 284
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 136
BseDI CCNNGG 3 cut(s) 141, 231, 305
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 1 cut(s) 480
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 3 cut(s) 189, 336, 381
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 2 cut(s) 231, 315
BshFI GGCC 3 cut(s) 230, 304, 310
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 136
BsiEI CGRYCG 2 cut(s) 231, 315
BsiSI CCGG 2 cut(s) 87, 110
BsmAI GTCTC 1 cut(s) 257
BsmBI CGTCTC 1 cut(s) 257
BsnI GGCC 3 cut(s) 230, 304, 310
Bsp143I GATC 3 cut(s) 133, 137, 150
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 4 cut(s) 9, 159, 213, 227
BspANI GGCC 3 cut(s) 230, 304, 310
BspCNI CTCAG 1 cut(s) 426
BspDI ATCGAT 1 cut(s) 136
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 366
BspPI GGATC 2 cut(s) 132, 141
BspT107I GGYRCC 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 480
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 3 cut(s) 141, 231, 305
BssMI GATC 3 cut(s) 133, 137, 150
BssNI GRCGYC 4 cut(s) 93, 266, 297, 404
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 376
BstACI GRCGYC 4 cut(s) 93, 266, 297, 404
BstDEI CTNAG 2 cut(s) 240, 434
BstDSI CCRYGG 1 cut(s) 231
BstFNI CGCG 1 cut(s) 275
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 3 cut(s) 136, 140, 153
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 3 cut(s) 133, 137, 150
BstMCI CGRYCG 2 cut(s) 231, 315
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 1 cut(s) 337
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 3 cut(s) 189, 336, 381
BstV2I GAAGAC 2 cut(s) 377, 407
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 136
BsuRI GGCC 3 cut(s) 230, 304, 310
BsuTUI ATCGAT 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 231
BtrI CACGTC 1 cut(s) 148
CfoI GCGC 1 cut(s) 275
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 477
ClaI ATCGAT 1 cut(s) 136
CseI GACGC 2 cut(s) 4, 274
CviAII CATG 2 cut(s) 19, 361
DdeI CTNAG 2 cut(s) 240, 434
DpnI GATC 3 cut(s) 135, 139, 152
DpnII GATC 3 cut(s) 133, 137, 150
EaeI YGGCCR 1 cut(s) 228
EagI CGGCCG 1 cut(s) 228
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 477
Eco52I CGGCCG 1 cut(s) 228
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 2 cut(s) 22, 364
FaiI YATR 5 cut(s) 20, 104, 155, 362, 431
FatI CATG 2 cut(s) 18, 360
FauI CCCGC 2 cut(s) 2, 152
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 2 cut(s) 407, 461
Fnu4HI GCNGC 5 cut(s) 178, 213, 228, 350, 395
Fsp4HI GCNGC 5 cut(s) 178, 213, 228, 350, 395
GlaI GCGC 1 cut(s) 274
GluI GCNGC 5 cut(s) 178, 213, 228, 350, 395
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 3 cut(s) 230, 304, 310
HapII CCGG 2 cut(s) 87, 110
HgaI GACGC 2 cut(s) 4, 274
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 4 cut(s) 93, 266, 297, 404
Hin1II CATG 2 cut(s) 22, 364
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 2 cut(s) 191, 418
HpaII CCGG 2 cut(s) 87, 110
Hpy166II GTNNAC 3 cut(s) 220, 408, 462
Hpy188I TCNGA 1 cut(s) 423
Hpy188III TCNNGA 1 cut(s) 122
Hpy8I GTNNAC 3 cut(s) 220, 408, 462
Hpy99I CGWCG 6 cut(s) 52, 95, 98, 409, 412, 475
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 4 cut(s) 93, 147, 297, 404
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 2 cut(s) 240, 434
HpySE526I ACGT 4 cut(s) 93, 147, 297, 404
Hsp92I GRCGYC 4 cut(s) 93, 266, 297, 404
Hsp92II CATG 2 cut(s) 22, 364
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 3 cut(s) 133, 137, 150
LpnPI CCDG 9 cut(s) 54, 74, 100, 107, 123, 241, 258, 286, 299
Lsp1109I GCAGC 3 cut(s) 189, 336, 381
MaeII ACGT 4 cut(s) 93, 147, 297, 404
MalI GATC 3 cut(s) 135, 139, 152
MboI GATC 3 cut(s) 133, 137, 150
MboII GAAGA 3 cut(s) 382, 412, 427
MluCI AATT 1 cut(s) 355
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 6 cut(s) 41, 178, 217, 300, 335, 468
MspI CCGG 2 cut(s) 87, 110
MspR9I CCNGG 1 cut(s) 110
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 1 cut(s) 236
NciI CCSGG 1 cut(s) 110
NdeII GATC 3 cut(s) 133, 137, 150
NlaIII CATG 2 cut(s) 22, 364
NlaIV GGNNCC 1 cut(s) 366
NmeAIII GCCGAG 1 cut(s) 330
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 272
PfeI GAWTC 1 cut(s) 418
PflFI GACNNNGTC 1 cut(s) 475
PkrI GCNGC 5 cut(s) 179, 214, 229, 351, 396
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 1 cut(s) 477
PsyI GACNNNGTC 1 cut(s) 475
SalI GTCGAC 1 cut(s) 406
SatI GCNGC 5 cut(s) 178, 213, 228, 350, 395
Sau3AI GATC 3 cut(s) 133, 137, 150
Sau96I GGNCC 1 cut(s) 477
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 1 cut(s) 110
SfcI CTRYAG 1 cut(s) 337
SgrAI CRCCGGYG 1 cut(s) 86
SgrDI CGTCGACG 1 cut(s) 406
SinI GGWCC 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 172
SmoI CTYRAG 1 cut(s) 172
Sse9I AATT 1 cut(s) 355
SsiI CCGC 4 cut(s) 9, 159, 213, 227
SspI AATATT 1 cut(s) 454
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 4 cut(s) 96, 150, 300, 407
TaqI TCGA 3 cut(s) 136, 189, 407
TaqII GACCGA 1 cut(s) 329
TasI AATT 1 cut(s) 355
TauI GCSGC 2 cut(s) 215, 230
TfiI GAWTC 1 cut(s) 418
TseI GCWGC 3 cut(s) 177, 349, 394
TspDTI ATGAA 1 cut(s) 349
Tth111I GACNNNGTC 1 cut(s) 475
VpaK11BI GGWCC 1 cut(s) 477
XapI RAATTY 1 cut(s) 355
XmiI GTMKAC 2 cut(s) 407, 461
ZraI GACGTC 3 cut(s) 94, 298, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.