Rw3G025000

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000677
Physical Location & Seq
Reverse (-)
32709271 .. 32709660
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025000.1

Sequence Viewer

Length: 390 bp
ATGCACCCGGACGGGTATCTAGAGATAAAGGATCGATCTAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCCGCCGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGATGCCGTGCGCATTTGTCAGCCTGAAAGCTGATGTCAGGCCGAGGCCGACAGAGAAGGAGATAATAGAGTTTTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCAAAGACGGTGGTGTTCAAGGACGAGCTGTCGAAGACGTCGACGGGGAAGATTCAGAAGTCTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACGTCAACAAAGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

129

Amino Acids

14.32

Weight (kDa)

8.55

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 28 - 105 3.7e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 305
Acc16I TGCGCA 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 262
AccI GTMKAC 2 cut(s) 305, 359
AciI CCGC 3 cut(s) 57, 111, 125
AclWI GGATC 1 cut(s) 39
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 253
AcyI GRCGYC 1 cut(s) 302
AflII CTTAAG 1 cut(s) 70
AgsI TTSAA 1 cut(s) 283
AhdI GACNNNNNGTC 1 cut(s) 292
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 3 cut(s) 191, 247, 292
AluI AGCT 3 cut(s) 191, 247, 292
AlwI GGATC 1 cut(s) 39
AoxI GGCC 3 cut(s) 126, 200, 206
ApeKI GCWGC 2 cut(s) 75, 247
ApoI RAATTY 1 cut(s) 253
AspLEI GCGC 1 cut(s) 173
AspS9I GGNCC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 375
BanI GGYRCC 1 cut(s) 262
BbsI GAAGAC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 87, 234
BceAI ACGGC 3 cut(s) 98, 113, 151
BclI TGATCA 1 cut(s) 48
BcnI CCSGG 1 cut(s) 8
BfaI CTAG 1 cut(s) 20
BfrI CTTAAG 1 cut(s) 70
BglI GCCNNNNNGGC 1 cut(s) 134
BisI GCNGC 4 cut(s) 76, 111, 126, 248
BlpI GCTNAGC 1 cut(s) 138
BlsI GCNGC 4 cut(s) 77, 112, 127, 249
Bme1390I CCNGG 1 cut(s) 8
Bme18I GGWCC 1 cut(s) 375
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 8
BmsI GCATC 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 305
Bpu1102I GCTNAGC 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 8
Bsa29I ATCGAT 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 129, 203
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 152, 182
Bse1I ACTGG 1 cut(s) 378
BseCI ATCGAT 1 cut(s) 34
BseDI CCNNGG 2 cut(s) 129, 203
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 378
BseX3I CGGCCG 1 cut(s) 126
BseXI GCAGC 2 cut(s) 87, 234
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 3 cut(s) 128, 202, 208
BshNI GGYRCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 34
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 8
BsnI GGCC 3 cut(s) 128, 202, 208
Bsp143I GATC 3 cut(s) 31, 35, 48
Bsp1720I GCTNAGC 1 cut(s) 138
BspACI CCGC 3 cut(s) 57, 111, 125
BspANI GGCC 3 cut(s) 128, 202, 208
BspCNI CTCAG 1 cut(s) 324
BspDI ATCGAT 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 262
BspTI CTTAAG 1 cut(s) 70
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 2 cut(s) 129, 203
BssMI GATC 3 cut(s) 31, 35, 48
BssNI GRCGYC 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 274
BstACI GRCGYC 1 cut(s) 302
BstAFI CTTAAG 1 cut(s) 70
BstDEI CTNAG 3 cut(s) 39, 138, 332
BstDSI CCRYGG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 34, 38, 51
BstMBI GATC 3 cut(s) 31, 35, 48
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 87, 234
BstV2I GAAGAC 1 cut(s) 305
BstZI CGGCCG 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 34
BsuRI GGCC 3 cut(s) 128, 202, 208
BsuTUI ATCGAT 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 46
CfoI GCGC 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 375
ClaI ATCGAT 1 cut(s) 34
CspCI CAANNNNNGTGG 2 cut(s) 255, 290
CviAII CATG 1 cut(s) 259
CviJI RGCY 9 cut(s) 128, 137, 142, 183, 191, 202, 208, 247, 292
CviKI_1 RGCY 9 cut(s) 128, 137, 142, 183, 191, 202, 208, 247, 292
DdeI CTNAG 3 cut(s) 39, 138, 332
DpnI GATC 3 cut(s) 33, 37, 50
DpnII GATC 3 cut(s) 31, 35, 48
DriI GACNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 126
EagI CGGCCG 1 cut(s) 126
Eam1105I GACNNNNNGTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 375
Eco52I CGGCCG 1 cut(s) 126
FaeI CATG 1 cut(s) 262
FaiI YATR 3 cut(s) 53, 260, 329
FatI CATG 1 cut(s) 258
FauI CCCGC 1 cut(s) 50
FbaI TGATCA 1 cut(s) 48
FblI GTMKAC 2 cut(s) 305, 359
Fnu4HI GCNGC 4 cut(s) 76, 111, 126, 248
Fsp4HI GCNGC 4 cut(s) 76, 111, 126, 248
FspAI RTGCGCAY 1 cut(s) 172
FspBI CTAG 1 cut(s) 20
FspI TGCGCA 1 cut(s) 172
GlaI GCGC 1 cut(s) 172
GluI GCNGC 4 cut(s) 76, 111, 126, 248
HaeIII GGCC 3 cut(s) 128, 202, 208
HapII CCGG 1 cut(s) 8
HhaI GCGC 1 cut(s) 173
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 1 cut(s) 262
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HincII GTYRAC 2 cut(s) 306, 366
HindII GTYRAC 2 cut(s) 306, 366
HinfI GANTC 2 cut(s) 89, 316
HpaII CCGG 1 cut(s) 8
Hpy166II GTNNAC 4 cut(s) 118, 306, 360, 366
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 4 cut(s) 118, 306, 360, 366
Hpy99I CGWCG 2 cut(s) 307, 310
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 3 cut(s) 45, 302, 362
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 3 cut(s) 39, 138, 332
HpySE526I ACGT 3 cut(s) 45, 302, 362
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 1 cut(s) 262
HspAI GCGC 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 3 cut(s) 31, 35, 48
LpnPI CCDG 5 cut(s) 21, 139, 156, 184, 197
Lsp1109I GCAGC 2 cut(s) 87, 234
LweI GCATC 1 cut(s) 153
MaeI CTAG 1 cut(s) 20
MaeII ACGT 3 cut(s) 45, 302, 362
MalI GATC 3 cut(s) 33, 37, 50
MboI GATC 3 cut(s) 31, 35, 48
MboII GAAGA 2 cut(s) 310, 325
MluCI AATT 1 cut(s) 253
MlyI GAGTC 1 cut(s) 98
MnlI CCTC 5 cut(s) 76, 115, 198, 233, 366
MseI TTAA 1 cut(s) 71
MspCI CTTAAG 1 cut(s) 70
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 8
NdeII GATC 3 cut(s) 31, 35, 48
NlaIII CATG 1 cut(s) 262
NlaIV GGNNCC 1 cut(s) 264
NmeAIII GCCGAG 1 cut(s) 228
NsbI TGCGCA 1 cut(s) 172
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 302
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 4 cut(s) 77, 112, 127, 249
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 1 cut(s) 375
SalI GTCGAC 1 cut(s) 304
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 4 cut(s) 76, 111, 126, 248
Sau3AI GATC 3 cut(s) 31, 35, 48
Sau96I GGNCC 1 cut(s) 375
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 8
SetI ASST 8 cut(s) 48, 87, 193, 249, 294, 305, 365, 377
SfaNI GCATC 1 cut(s) 153
SgrDI CGTCGACG 1 cut(s) 304
SinI GGWCC 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
Sse9I AATT 1 cut(s) 253
SsiI CCGC 3 cut(s) 57, 111, 125
SspI AATATT 1 cut(s) 352
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 3 cut(s) 48, 305, 365
TaqI TCGA 4 cut(s) 34, 87, 296, 305
TasI AATT 1 cut(s) 253
TauI GCSGC 2 cut(s) 113, 128
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TseI GCWGC 2 cut(s) 75, 247
TspDTI ATGAA 1 cut(s) 247
Vha464I CTTAAG 1 cut(s) 70
VpaK11BI GGWCC 1 cut(s) 375
XapI RAATTY 1 cut(s) 253
XbaI TCTAGA 1 cut(s) 19
XmiI GTMKAC 2 cut(s) 305, 359
XspI CTAG 1 cut(s) 20
ZraI GACGTC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.