Rmu_sc0005285.1_g000013

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005285.1
OG0000677
Physical Location & Seq
Forward (+)
56404 .. 57156
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005285.1_g000013.1.cds

Sequence Viewer

Length: 753 bp
atgaccgccggagctccgcctcccgcagcggttctgtctcggactgagtcgctgggattcgtggtgagccacgggtacgggttaacggagacggcggggctggtggtgtcgtgcgcgtggaagcccgggtggaataagttaccggcgacggagagggcgaggctgaagtcgaggcagggggtgaggacggtggccatgacccgggtggacgtggtggatccggatacgggtgtgccggttaaacgcgacggttcgtcgcttggggaggtggttttcagaggcgggtgtgtgatgttgggttatctgaaagacccaaaaggcacggccagttgcatgaaagacggttggttttacaccggcgacgtggcggtgatgcacccggacgggtatctggagatcaaggatagatccaaggacgtgatcataagcgggggagagaacttgagcagcgtggaggtggagtcggttctctacaccaactcggcggtgaacgaggcggccgtggtggcgaggcctgacgagttctggggggagacgccgtgcgcgtttgtgagcctgaaagatgacgtggggcggaggccgacagagaaggagatgatagagtattgcagaggaaagctgccgaatttcatggtgccgaagacggtggtgtttattgaggagctgccgaagacgtcgacggggaaaattcagaagttcgtgctgagagagatggccaaggccttggggtccacaaggctgagtcggatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

250

Amino Acids

27.17

Weight (kDa)

8.53

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 677
AccB1I GGYRCC 1 cut(s) 634
AccI GTMKAC 1 cut(s) 677
AccII CGCG 3 cut(s) 116, 246, 545
AccIII TCCGGA 1 cut(s) 220
AclWI GGATC 3 cut(s) 212, 225, 402
AcoI YGGCCR 4 cut(s) 192, 324, 498, 714
AcsI RAATTY 2 cut(s) 625, 687
AcuI CTGAAG 1 cut(s) 185
AcyI GRCGYC 2 cut(s) 536, 674
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 201, 227
AhdI GACNNNNNGTC 1 cut(s) 253
AjiI CACGTC 4 cut(s) 211, 364, 418, 568
AluBI AGCT 3 cut(s) 14, 619, 664
AluI AGCT 3 cut(s) 14, 619, 664
Alw21I GWGCWC 1 cut(s) 16
Alw26I GTCTC 3 cut(s) 42, 83, 527
AlwI GGATC 3 cut(s) 212, 225, 402
Ama87I CYCGRG 2 cut(s) 125, 201
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 7 cut(s) 192, 324, 498, 512, 578, 714, 720
ApeKI GCWGC 4 cut(s) 26, 447, 619, 664
ApoI RAATTY 2 cut(s) 625, 687
AspLEI GCGC 2 cut(s) 116, 545
AspS9I GGNCC 1 cut(s) 729
AsuC2I CCSGG 5 cut(s) 126, 127, 202, 203, 380
AsuHPI GGTGA 4 cut(s) 76, 193, 382, 499
AvaI CYCGRG 2 cut(s) 125, 201
AvaII GGWCC 1 cut(s) 729
BaeI ACNNNNGTAYC 2 cut(s) 216, 249
BalI TGGCCA 2 cut(s) 194, 716
BamHI GGATCC 1 cut(s) 217
BanI GGYRCC 1 cut(s) 634
BanII GRGCYC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 647, 677
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 38, 459, 606, 651
BccI CCATC 1 cut(s) 706
BceAI ACGGC 4 cut(s) 108, 339, 485, 523
BciVI GTATCC 1 cut(s) 217
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 5 cut(s) 126, 127, 202, 203, 380
BcoDI GTCTC 3 cut(s) 42, 83, 527
BfuI GTATCC 1 cut(s) 217
BglI GCCNNNNNGGC 1 cut(s) 506
BisI GCNGC 5 cut(s) 27, 448, 498, 620, 665
BlsI GCNGC 5 cut(s) 28, 449, 499, 621, 666
Bme1390I CCNGG 5 cut(s) 126, 127, 202, 203, 380
Bme18I GGWCC 1 cut(s) 729
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 2 cut(s) 125, 201
BmgBI CACGTC 4 cut(s) 211, 364, 418, 568
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 3 cut(s) 219, 636, 730
BmrFI CCNGG 5 cut(s) 126, 127, 202, 203, 380
BmsI GCATC 1 cut(s) 363
BpiI GAAGAC 2 cut(s) 647, 677
BpmI CTGGAG 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 463
BpuMI CCSGG 5 cut(s) 126, 127, 202, 203, 380
BsaHI GRCGYC 2 cut(s) 536, 674
BsaJI CCNNGG 7 cut(s) 70, 125, 201, 411, 501, 717, 723
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 4 cut(s) 446, 476, 524, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 227
Bse118I RCCGGY 3 cut(s) 142, 235, 356
Bse1I ACTGG 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 220
BseDI CCNNGG 7 cut(s) 70, 125, 201, 411, 501, 717, 723
BseGI GGATG 1 cut(s) 753
BseLI CCNNNNNNNGG 2 cut(s) 201, 227
BseMII CTCAG 3 cut(s) 36, 695, 731
BseNI ACTGG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 674
BseX3I CGGCCG 1 cut(s) 498
BseXI GCAGC 4 cut(s) 38, 459, 606, 651
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 3 cut(s) 116, 246, 545
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 7 cut(s) 194, 326, 500, 514, 580, 716, 722
BshNI GGYRCC 1 cut(s) 634
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 1 cut(s) 16
BsiHKCI CYCGRG 2 cut(s) 125, 201
BsiSI CCGG 8 cut(s) 9, 126, 143, 202, 221, 236, 357, 380
BslI CCNNNNNNNGG 2 cut(s) 201, 227
BsmAI GTCTC 3 cut(s) 42, 83, 527
BsmBI CGTCTC 2 cut(s) 83, 527
BsnI GGCC 7 cut(s) 194, 326, 500, 514, 580, 716, 722
BsoBI CYCGRG 2 cut(s) 125, 201
Bsp1286I GDGCHC 1 cut(s) 16
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 4 cut(s) 217, 396, 407, 420
BspANI GGCC 7 cut(s) 194, 326, 500, 514, 580, 716, 722
BspCNI CTCAG 3 cut(s) 37, 696, 732
BspEI TCCGGA 1 cut(s) 220
BspFNI CGCG 3 cut(s) 116, 246, 545
BspLI GGNNCC 3 cut(s) 219, 636, 730
BspPI GGATC 3 cut(s) 212, 225, 402
BspT107I GGYRCC 1 cut(s) 634
BsrFI RCCGGY 3 cut(s) 142, 235, 356
BsrI ACTGG 1 cut(s) 327
BssAI RCCGGY 3 cut(s) 142, 235, 356
BssECI CCNNGG 7 cut(s) 70, 125, 201, 411, 501, 717, 723
BssMI GATC 4 cut(s) 217, 396, 407, 420
BssNI GRCGYC 2 cut(s) 536, 674
BssT1I CCWWGG 3 cut(s) 411, 717, 723
Bst4CI ACNGT 4 cut(s) 190, 251, 344, 646
BstACI GRCGYC 2 cut(s) 536, 674
BstDEI CTNAG 3 cut(s) 45, 704, 740
BstDSI CCRYGG 2 cut(s) 70, 501
BstF5I GGATG 1 cut(s) 753
BstFNI CGCG 3 cut(s) 116, 246, 545
BstHHI GCGC 2 cut(s) 116, 545
BstKTI GATC 4 cut(s) 220, 399, 410, 423
BstMAI GTCTC 3 cut(s) 42, 83, 527
BstMBI GATC 4 cut(s) 217, 396, 407, 420
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 1 cut(s) 506
BstSCI CCNGG 5 cut(s) 124, 125, 200, 201, 378
BstUI CGCG 3 cut(s) 116, 246, 545
BstV1I GCAGC 4 cut(s) 38, 459, 606, 651
BstV2I GAAGAC 2 cut(s) 647, 677
BstX2I RGATCY 2 cut(s) 217, 407
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 2 cut(s) 217, 407
BstZI CGGCCG 1 cut(s) 498
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 7 cut(s) 194, 326, 500, 514, 580, 716, 722
BtgI CCRYGG 2 cut(s) 70, 501
BtrI CACGTC 4 cut(s) 211, 364, 418, 568
BtsCI GGATG 1 cut(s) 753
CfoI GCGC 2 cut(s) 116, 545
Cfr10I RCCGGY 3 cut(s) 142, 235, 356
Cfr13I GGNCC 1 cut(s) 729
Cfr9I CCCGGG 2 cut(s) 125, 201
CseI GACGC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 76
CviAII CATG 3 cut(s) 196, 334, 631
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 3 cut(s) 45, 704, 740
DpnI GATC 4 cut(s) 219, 398, 409, 422
DpnII GATC 4 cut(s) 217, 396, 407, 420
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 4 cut(s) 192, 324, 498, 714
EagI CGGCCG 1 cut(s) 498
Eam1105I GACNNNNNGTC 1 cut(s) 253
EciI GGCGGA 2 cut(s) 6, 589
Ecl136II GAGCTC 1 cut(s) 14
EclXI CGGCCG 1 cut(s) 498
Eco130I CCWWGG 3 cut(s) 411, 717, 723
Eco147I AGGCCT 2 cut(s) 514, 722
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 729
Eco52I CGGCCG 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 14
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 2 cut(s) 125, 201
EcoICRI GAGCTC 1 cut(s) 14
EcoT14I CCWWGG 3 cut(s) 411, 717, 723
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 3 cut(s) 411, 717, 723
Esp3I CGTCTC 2 cut(s) 83, 527
FaeI CATG 3 cut(s) 199, 337, 634
FaiI YATR 4 cut(s) 197, 335, 425, 632
FatI CATG 3 cut(s) 195, 333, 630
FauI CCCGC 4 cut(s) 31, 88, 275, 422
FbaI TGATCA 1 cut(s) 420
FblI GTMKAC 1 cut(s) 677
Fnu4HI GCNGC 5 cut(s) 27, 448, 498, 620, 665
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 5 cut(s) 27, 448, 498, 620, 665
GlaI GCGC 2 cut(s) 115, 544
GluI GCNGC 5 cut(s) 27, 448, 498, 620, 665
GsaI CCCAGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 413
HaeIII GGCC 7 cut(s) 194, 326, 500, 514, 580, 716, 722
HapII CCGG 8 cut(s) 9, 126, 143, 202, 221, 236, 357, 380
HgaI GACGC 1 cut(s) 544
HhaI GCGC 2 cut(s) 116, 545
Hin1I GRCGYC 2 cut(s) 536, 674
Hin1II CATG 3 cut(s) 199, 337, 634
Hin6I GCGC 2 cut(s) 114, 543
HinP1I GCGC 2 cut(s) 114, 543
HincII GTYRAC 2 cut(s) 84, 678
HindII GTYRAC 2 cut(s) 84, 678
HinfI GANTC 4 cut(s) 47, 57, 461, 742
HpaI GTTAAC 1 cut(s) 84
HpaII CCGG 8 cut(s) 9, 126, 143, 202, 221, 236, 357, 380
HphI GGTGA 4 cut(s) 76, 193, 382, 499
Hpy166II GTNNAC 5 cut(s) 84, 208, 490, 678, 732
Hpy188I TCNGA 5 cut(s) 42, 278, 306, 693, 747
Hpy188III TCNNGA 2 cut(s) 221, 392
Hpy8I GTNNAC 5 cut(s) 84, 208, 490, 678, 732
Hpy99I CGWCG 6 cut(s) 151, 251, 259, 365, 679, 682
HpyAV CCTTC 1 cut(s) 583
HpyCH4III ACNGT 4 cut(s) 190, 251, 344, 646
HpyCH4IV ACGT 5 cut(s) 210, 363, 417, 567, 674
HpyCH4V TGCA 3 cut(s) 333, 376, 609
HpyF10VI GCNNNNNNNGC 1 cut(s) 506
HpyF3I CTNAG 3 cut(s) 45, 704, 740
HpySE526I ACGT 5 cut(s) 210, 363, 417, 567, 674
Hsp92I GRCGYC 2 cut(s) 536, 674
Hsp92II CATG 3 cut(s) 199, 337, 634
HspAI GCGC 2 cut(s) 114, 543
Kpn2I TCCGGA 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 84
Kzo9I GATC 4 cut(s) 217, 396, 407, 420
LmnI GCTCC 3 cut(s) 11, 19, 661
Lsp1109I GCAGC 4 cut(s) 38, 459, 606, 651
LweI GCATC 1 cut(s) 363
MaeII ACGT 5 cut(s) 210, 363, 417, 567, 674
MaeIII GTNAC 1 cut(s) 138
MalI GATC 4 cut(s) 219, 398, 409, 422
MboI GATC 4 cut(s) 217, 396, 407, 420
MboII GAAGA 2 cut(s) 652, 682
MflI RGATCY 2 cut(s) 217, 407
MhlI GDGCHC 1 cut(s) 16
MlsI TGGCCA 2 cut(s) 194, 716
MluCI AATT 2 cut(s) 625, 687
MluNI TGGCCA 2 cut(s) 194, 716
MlyI GAGTC 3 cut(s) 56, 470, 751
MmeI TCCRAC 1 cut(s) 725
Mox20I TGGCCA 2 cut(s) 194, 716
MroI TCCGGA 1 cut(s) 220
MscI TGGCCA 2 cut(s) 194, 716
MseI TTAA 2 cut(s) 83, 240
Msp20I TGGCCA 2 cut(s) 194, 716
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 8 cut(s) 9, 126, 143, 202, 221, 236, 357, 380
MspR9I CCNGG 5 cut(s) 126, 127, 202, 203, 380
MvnI CGCG 3 cut(s) 116, 246, 545
MwoI GCNNNNNNNGC 1 cut(s) 506
NciI CCSGG 5 cut(s) 126, 127, 202, 203, 380
NdeII GATC 4 cut(s) 217, 396, 407, 420
NlaIII CATG 3 cut(s) 199, 337, 634
NlaIV GGNNCC 3 cut(s) 219, 636, 730
NmeAIII GCCGAG 1 cut(s) 461
PceI AGGCCT 2 cut(s) 514, 722
PcsI WCGNNNNNNNCGW 3 cut(s) 155, 498, 542
PfeI GAWTC 1 cut(s) 57
PflFI GACNNNGTC 1 cut(s) 46
PkrI GCNGC 5 cut(s) 28, 449, 499, 621, 666
PleI GAGTC 3 cut(s) 55, 469, 750
PpsI GAGTC 3 cut(s) 55, 469, 750
Psp124BI GAGCTC 1 cut(s) 16
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 219, 636, 730
PspPI GGNCC 1 cut(s) 729
PsuI RGATCY 2 cut(s) 217, 407
PsyI GACNNNGTC 1 cut(s) 46
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SacI GAGCTC 1 cut(s) 16
SalI GTCGAC 1 cut(s) 676
SaqAI TTAA 2 cut(s) 83, 240
SatI GCNGC 5 cut(s) 27, 448, 498, 620, 665
Sau3AI GATC 4 cut(s) 217, 396, 407, 420
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 3 cut(s) 56, 470, 751
ScrFI CCNGG 5 cut(s) 126, 127, 202, 203, 380
SduI GDGCHC 1 cut(s) 16
SfaNI GCATC 1 cut(s) 363
SgrAI CRCCGGYG 1 cut(s) 356
SgrDI CGTCGACG 1 cut(s) 676
SinI GGWCC 1 cut(s) 729
SmaI CCCGGG 2 cut(s) 127, 203
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
Sse9I AATT 2 cut(s) 625, 687
SseBI AGGCCT 2 cut(s) 514, 722
SstI GAGCTC 1 cut(s) 16
StuI AGGCCT 2 cut(s) 514, 722
StyD4I CCNGG 5 cut(s) 124, 125, 200, 201, 378
StyI CCWWGG 3 cut(s) 411, 717, 723
TaaI ACNGT 4 cut(s) 190, 251, 344, 646
TaiI ACGT 5 cut(s) 213, 366, 420, 570, 677
TaqI TCGA 2 cut(s) 170, 677
TasI AATT 2 cut(s) 625, 687
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 1 cut(s) 57
Tru1I TTAA 2 cut(s) 83, 240
Tru9I TTAA 2 cut(s) 83, 240
TseI GCWGC 4 cut(s) 26, 447, 619, 664
TspDTI ATGAA 2 cut(s) 350, 619
TspGWI ACGGA 2 cut(s) 101, 164
TspMI CCCGGG 2 cut(s) 125, 201
Tth111I GACNNNGTC 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 729
XapI RAATTY 2 cut(s) 625, 687
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmaI CCCGGG 2 cut(s) 125, 201
XmiI GTMKAC 1 cut(s) 677
ZraI GACGTC 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.