Rmu_sc0009101.1_g000001

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009101.1
OG0000677
Physical Location & Seq
Reverse (-)
1270 .. 3171
1902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009101.1_g000001.1.cds

Sequence Viewer

Length: 807 bp
atgacccacgtcgacgtggtggatccgaatacgggtgcgccggttaaaagcgacggttcatcgctcggggaggtggttttcaaaggcgggtctgtgatgttgggttacctgaaagacccaaaaggcacggctagttgcatgaaagacggtttgttttataccggcgacgtggcagtgatgcacccggacgggtatctggagatcaaggatcgatctaaggatgtgatcattagcgggggagagaacgtgagcagcatggaggtcgagtcggttctctacaccaacgcggcggtgaacgaggctgcagtggtggctaggcctgacgagttttggggggagacgccgtgcgcgtttgtgagcctgaaagctgacgtggcgcagaggccgacggagaaggagataatagaatattgcagggggaagttgccgcattacatggtgccgaagacggtggtgtttaaggaggagctgccgaagacttcgacggggaagattcagaagttcgtgctgagagagatggcggcccagtgttacttggttcgcatgtcgttgcggaatgggtacgccgtacgagtacgcagtaggccagcctctatgatacgcacatatgctcttagtttggttgaagctgacaaagagagcgagcaaggaattctgaagaagaaagttggaaggcctcttaggagcaagaataagaatcctcgtcctcaaaagaaggtgagtggggctcctttgcgtctcacttatcctactaatgttattgttcaagaggttagccctaaggataagggcaaaggaaagctctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

268

Amino Acids

29.45

Weight (kDa)

9.38

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 12
AccII CGCG 2 cut(s) 287, 350
AciI CCGC 7 cut(s) 87, 234, 287, 290, 428, 521, 553
AclWI GGATC 3 cut(s) 17, 30, 216
AcsI RAATTY 1 cut(s) 651
AcuI CTGAAG 1 cut(s) 677
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 3 cut(s) 563, 570, 576
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 3 cut(s) 82, 626, 767
AjiI CACGTC 4 cut(s) 10, 16, 169, 373
AluBI AGCT 4 cut(s) 368, 469, 629, 802
AluI AGCT 4 cut(s) 368, 469, 629, 802
Alw26I GTCTC 2 cut(s) 332, 743
AlwI GGATC 3 cut(s) 17, 30, 216
Ama87I CYCGRG 1 cut(s) 65
AoxI GGCC 5 cut(s) 317, 383, 522, 584, 674
ApeKI GCWGC 3 cut(s) 252, 302, 469
ApoI RAATTY 1 cut(s) 651
AspLEI GCGC 3 cut(s) 40, 350, 379
AspS9I GGNCC 1 cut(s) 523
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 304, 730
AvaI CYCGRG 1 cut(s) 65
AxyI CCTNAGG 1 cut(s) 780
BamHI GGATCC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 730
BbsI GAAGAC 2 cut(s) 452, 482
BbvI GCAGC 3 cut(s) 264, 289, 456
BccI CCATC 1 cut(s) 511
BceAI ACGGC 3 cut(s) 144, 328, 551
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BclI TGATCA 1 cut(s) 225
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 332, 743
BfaI CTAG 2 cut(s) 132, 315
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 6 cut(s) 253, 288, 303, 428, 470, 522
BlsI GCNGC 6 cut(s) 254, 289, 304, 429, 471, 523
Bme1390I CCNGG 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 65
BmgBI CACGTC 4 cut(s) 10, 16, 169, 373
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 3 cut(s) 24, 441, 729
BmrFI CCNGG 1 cut(s) 185
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 520
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 452, 482
BplI GAGNNNNNCTC 2 cut(s) 712, 744
BpmI CTGGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 341
BsaXI ACNNNNNCTCC 6 cut(s) 231, 251, 261, 281, 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 2 cut(s) 40, 161
Bse1I ACTGG 1 cut(s) 526
Bse21I CCTNAGG 1 cut(s) 780
BseCI ATCGAT 1 cut(s) 211
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 1 cut(s) 526
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 3 cut(s) 264, 289, 456
Bsh1236I CGCG 2 cut(s) 287, 350
BshFI GGCC 5 cut(s) 319, 385, 524, 586, 676
BshNI GGYRCC 1 cut(s) 439
BshVI ATCGAT 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 3 cut(s) 41, 162, 185
BsiWI CGTACG 1 cut(s) 568
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 332, 743
BsmBI CGTCTC 2 cut(s) 332, 743
BsnI GGCC 5 cut(s) 319, 385, 524, 586, 676
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 5 cut(s) 22, 201, 208, 212, 225
BspACI CCGC 7 cut(s) 87, 234, 287, 290, 428, 521, 553
BspANI GGCC 5 cut(s) 319, 385, 524, 586, 676
BspCNI CTCAG 1 cut(s) 501
BspDI ATCGAT 1 cut(s) 211
BspFNI CGCG 2 cut(s) 287, 350
BspLI GGNNCC 3 cut(s) 24, 441, 729
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 3 cut(s) 17, 30, 216
BspT107I GGYRCC 1 cut(s) 439
BsrFI RCCGGY 2 cut(s) 40, 161
BsrI ACTGG 1 cut(s) 526
BssAI RCCGGY 2 cut(s) 40, 161
BssMI GATC 5 cut(s) 22, 201, 208, 212, 225
BssNI GRCGYC 1 cut(s) 341
Bst4CI ACNGT 3 cut(s) 56, 149, 451
BstACI GRCGYC 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 588, 644
BstDEI CTNAG 5 cut(s) 216, 509, 614, 680, 780
BstEII GGTNACC 1 cut(s) 104
BstF5I GGATG 1 cut(s) 226
BstFNI CGCG 2 cut(s) 287, 350
BstHHI GCGC 3 cut(s) 40, 350, 379
BstKTI GATC 5 cut(s) 25, 204, 211, 215, 228
BstMAI GTCTC 2 cut(s) 332, 743
BstMBI GATC 5 cut(s) 22, 201, 208, 212, 225
BstMWI GCNNNNNNNGC 2 cut(s) 311, 374
BstNSI RCATGY 1 cut(s) 547
BstPAI GACNNNNGTC 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 2 cut(s) 287, 350
BstV1I GCAGC 3 cut(s) 264, 289, 456
BstV2I GAAGAC 2 cut(s) 452, 482
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 211
Bsu36I CCTNAGG 1 cut(s) 780
BsuRI GGCC 5 cut(s) 319, 385, 524, 586, 676
BsuTUI ATCGAT 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 45
BtrI CACGTC 4 cut(s) 10, 16, 169, 373
BtsCI GGATG 1 cut(s) 226
BtsI GCAGTG 2 cut(s) 180, 312
BtsIMutI CAGTG 3 cut(s) 180, 312, 533
Cac8I GCNNGC 2 cut(s) 588, 644
CfoI GCGC 3 cut(s) 40, 350, 379
Cfr10I RCCGGY 2 cut(s) 40, 161
Cfr13I GGNCC 1 cut(s) 523
ClaI ATCGAT 1 cut(s) 211
CseI GACGC 2 cut(s) 349, 725
Csp6I GTAC 3 cut(s) 562, 569, 575
CviAII CATG 4 cut(s) 139, 256, 436, 544
CviQI GTAC 3 cut(s) 562, 569, 575
DdeI CTNAG 5 cut(s) 216, 509, 614, 680, 780
DpnI GATC 5 cut(s) 24, 203, 210, 214, 227
DpnII GATC 5 cut(s) 22, 201, 208, 212, 225
Eco147I AGGCCT 2 cut(s) 319, 676
Eco24I GRGCYC 1 cut(s) 730
Eco57I CTGAAG 1 cut(s) 677
Eco81I CCTNAGG 1 cut(s) 780
Eco88I CYCGRG 1 cut(s) 65
Eco91I GGTNACC 1 cut(s) 104
EcoO65I GGTNACC 1 cut(s) 104
EcoRI GAATTC 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 730
Esp3I CGTCTC 2 cut(s) 332, 743
FaeI CATG 4 cut(s) 142, 259, 439, 547
FaiI YATR 8 cut(s) 140, 159, 257, 437, 545, 596, 607, 609
FatI CATG 4 cut(s) 138, 255, 435, 543
FauI CCCGC 2 cut(s) 80, 227
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 6 cut(s) 253, 288, 303, 428, 470, 522
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 730
Fsp4HI GCNGC 6 cut(s) 253, 288, 303, 428, 470, 522
FspBI CTAG 2 cut(s) 132, 315
GlaI GCGC 3 cut(s) 39, 349, 378
GluI GCNGC 6 cut(s) 253, 288, 303, 428, 470, 522
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 5 cut(s) 319, 385, 524, 586, 676
HapII CCGG 3 cut(s) 41, 162, 185
HgaI GACGC 2 cut(s) 349, 725
HhaI GCGC 3 cut(s) 40, 350, 379
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 4 cut(s) 142, 259, 439, 547
Hin6I GCGC 3 cut(s) 38, 348, 377
HinP1I GCGC 3 cut(s) 38, 348, 377
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 3 cut(s) 266, 493, 697
HpaII CCGG 3 cut(s) 41, 162, 185
HphI GGTGA 2 cut(s) 304, 730
Hpy166II GTNNAC 2 cut(s) 13, 295
Hpy188I TCNGA 3 cut(s) 27, 498, 657
Hpy188III TCNNGA 2 cut(s) 197, 767
Hpy8I GTNNAC 2 cut(s) 13, 295
Hpy99I CGWCG 6 cut(s) 14, 17, 56, 170, 391, 487
HpyAV CCTTC 3 cut(s) 388, 666, 709
HpyCH4III ACNGT 3 cut(s) 56, 149, 451
HpyCH4IV ACGT 5 cut(s) 9, 15, 168, 246, 372
HpyCH4V TGCA 4 cut(s) 138, 181, 305, 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 374
HpyF3I CTNAG 5 cut(s) 216, 509, 614, 680, 780
HpySE526I ACGT 5 cut(s) 9, 15, 168, 246, 372
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 4 cut(s) 142, 259, 439, 547
HspAI GCGC 3 cut(s) 38, 348, 377
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 5 cut(s) 22, 201, 208, 212, 225
LmnI GCTCC 3 cut(s) 466, 684, 733
Lsp1109I GCAGC 3 cut(s) 264, 289, 456
LweI GCATC 1 cut(s) 168
MaeI CTAG 2 cut(s) 132, 315
MaeII ACGT 5 cut(s) 9, 15, 168, 246, 372
MaeIII GTNAC 2 cut(s) 104, 530
MalI GATC 5 cut(s) 24, 203, 210, 214, 227
MboI GATC 5 cut(s) 22, 201, 208, 212, 225
MboII GAAGA 5 cut(s) 457, 487, 502, 670, 673
MflI RGATCY 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 1 cut(s) 651
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 1 cut(s) 649
MseI TTAA 2 cut(s) 45, 459
MspI CCGG 3 cut(s) 41, 162, 185
MspR9I CCNGG 1 cut(s) 185
MvnI CGCG 2 cut(s) 287, 350
MwoI GCNNNNNNNGC 2 cut(s) 311, 374
NciI CCSGG 1 cut(s) 185
NdeI CATATG 1 cut(s) 607
NdeII GATC 5 cut(s) 22, 201, 208, 212, 225
NlaIII CATG 4 cut(s) 142, 259, 439, 547
NlaIV GGNNCC 3 cut(s) 24, 441, 729
NspI RCATGY 1 cut(s) 547
PceI AGGCCT 2 cut(s) 319, 676
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PfeI GAWTC 2 cut(s) 493, 697
Pfl23II CGTACG 1 cut(s) 568
PkrI GCNGC 6 cut(s) 254, 289, 304, 429, 471, 523
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PshAI GACNNNNGTC 1 cut(s) 8
PspEI GGTNACC 1 cut(s) 104
PspLI CGTACG 1 cut(s) 568
PspN4I GGNNCC 3 cut(s) 24, 441, 729
PspPI GGNCC 1 cut(s) 523
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 22
RsaI GTAC 3 cut(s) 563, 570, 576
RsaNI GTAC 3 cut(s) 562, 569, 575
SalI GTCGAC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 45, 459
SatI GCNGC 6 cut(s) 253, 288, 303, 428, 470, 522
Sau3AI GATC 5 cut(s) 22, 201, 208, 212, 225
Sau96I GGNCC 1 cut(s) 523
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 730
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 303
SgrDI CGTCGACG 1 cut(s) 11
Sse9I AATT 1 cut(s) 651
SseBI AGGCCT 2 cut(s) 319, 676
SsiI CCGC 7 cut(s) 87, 234, 287, 290, 428, 521, 553
SspI AATATT 1 cut(s) 410
SspMI CTAG 2 cut(s) 132, 315
StuI AGGCCT 2 cut(s) 319, 676
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 3 cut(s) 56, 149, 451
TaiI ACGT 5 cut(s) 12, 18, 171, 249, 375
TaqI TCGA 4 cut(s) 12, 211, 264, 482
TasI AATT 1 cut(s) 651
TauI GCSGC 3 cut(s) 290, 430, 524
TfiI GAWTC 2 cut(s) 493, 697
Tru1I TTAA 2 cut(s) 45, 459
Tru9I TTAA 2 cut(s) 45, 459
TscAI CASTG 3 cut(s) 180, 312, 533
TseI GCWGC 3 cut(s) 252, 302, 469
TspDTI ATGAA 2 cut(s) 48, 155
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 3 cut(s) 180, 312, 533
XapI RAATTY 1 cut(s) 651
XceI RCATGY 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 2 cut(s) 132, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.