MD17G1272700.v1.1

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000677
Physical Location & Seq
Forward (+)
33494718 .. 33496420
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1272700.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGAAGATCTGAAGCCCACGTCAGCAAACTCATCCCCTCTAACACCCTTGGGCTTTCTGGAAAGAGCAGCCACCGTCTACGGCGACTGCCCGTCCGTTATATACGGAGACACCACCTACACTTGGACACAGACCCACCGTCGATGTCTCCGCGTGGCTTCCTCTATATCATCCCTTGGTATCAAGACAGGCAACGTGGTGTCCGTTCTCTCCCCAAACATCCCAGCCATGTACGAGCTCCACTTCGCCGTGCCCATGTCCGGCGCCATCCTCAACAACATCAACACCCGCCTGGACAGCCGCACCGTCTCCGTACTCCTGCGCCACAGCGAGTCAAAACTCGTCTTCGTCGACTACGAATTGCAATCCCTAATCCTCGAAGCCATCTCTCTCTTCCCACCAAACACCCCCTCCCCTCGACTCGTCCTCATCGCCGGAGATTCCGGCGCGCCGTCACGCGCAGCACCACTCGATGACTTCCTTTGCACGTACGAGGACCTCGTGGACAGGGGCGACGAGGGGTTCGAGTGGGTCTCGCCGTCGAACGAGTGGGTTCCGATGACGCTGAATTACACGTCGGGGACCACATCGTCTCCCAAGGGAGTCGTTCACTCCCACAGAGGGGTCTTCATCATCGCCGTCGATTCCCTGATCGACTGGGGTGTACCCAAACAGCCGGTGTACCTGTGGACCCTACCGATGTTCCACGCTAACGGGTGGAGCTACCCCTACGGAATGGCAGCCGTCGGTGGGACCAACATCTGCGTCCGTAAGTTCGACGCCCAAATAATCTATTCCATGATCAAACGATACCGAGTTACCCACATGTGCGGTGCGCCGGTGGTGCTGAACATGCTCACAAACGTCCCGTCCGGAACCGAAAAGCTCGAGTACCCGGTTCACATCCTGACAGCCGGAGCTCCGCCGCCGGCTCCGGTTCTGTTCCGGACCGAGTCCCTGGGTTTCGTGGTGAGTCACGGGTATGGGCTGACCGAAACGGCGGGGCTGGTGGTGTCGTGCGCGTGGAAGAGGAAGTGGAATTTGTTGCCGGCGACTGAGAGAGCAAGGCTGAAGTCGAGGCAGGGAGTGAGGACGGTCGGGCTGACCCGAATGGACGTGGTAAACCCGGACTCGGGACTCAGCGTAAAACGCGACGGCACGTCGCTCGGCGAAGTCGTTTTGAGAGGCGGGTGCGTGATGCTAGGGTATTTGAAAGACCAATCGGGAACAGCCAAGTGCATGAAAGACGGGTGGTTTTACACGGGTGACGTGGGCGTGATGCATCCCGACGGCTATCTGGAAATTAAGGACAGATCCAAGGACGTTATTATAAGCGGGGGCGAGAATCTGAGCAGTGTGGAGGTGGAGTCGGTGCTGTATACGAACCCGGCTGTCAACGAGGCGGCGGTGGTGGCGAGGCCGGACGAGTTTTGGGGGGAGACGCCGTGTGCATTCGTGAGCTTGAAGAGTGAGGTGAGGCCGAAGCCGACGGAGAAGGAGATCATTGAGTATTGCAGGAAGAAGCTGCCGAAATACATGGTGCCAAAAACGGTGGTGTTTACGGACGAGCTTCCCAAGACATCGACCGGGAAGATTCAGAAGTTTGTGCTGAGACAGATGGCGAAGGGTTTGGGTTCGTCGAGGCCTAGTCGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

553

Amino Acids

60.55

Weight (kDa)

8.52

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 404 4.2e-71 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 9.1e-27 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1331
AasI GACNNNNNNGTC 1 cut(s) 589
AccB1I GGYRCC 2 cut(s) 263, 1540
AccI GTMKAC 3 cut(s) 78, 351, 1379
AccII CGCG 5 cut(s) 153, 449, 459, 1022, 1152
AccIII TCCGGA 2 cut(s) 872, 945
AclWI GGATC 1 cut(s) 1308
AcsI RAATTY 1 cut(s) 1039
AcuI CTGAAG 2 cut(s) 32, 1091
AcyI GRCGYC 3 cut(s) 264, 780, 1442
AfaI GTAC 6 cut(s) 233, 315, 491, 666, 683, 893
AfiI CCNNNNNNNGG 7 cut(s) 123, 260, 621, 622, 750, 1132, 1133
AflIII ACRYGT 2 cut(s) 573, 825
AgsI TTSAA 2 cut(s) 1213, 1465
AhdI GACNNNNNGTC 3 cut(s) 91, 138, 1159
AjiI CACGTC 5 cut(s) 21, 576, 1117, 1161, 1270
AjnI CCWGG 2 cut(s) 291, 957
AluBI AGCT 7 cut(s) 238, 723, 886, 920, 1461, 1525, 1570
AluI AGCT 7 cut(s) 238, 723, 886, 920, 1461, 1525, 1570
Alw21I GWGCWC 2 cut(s) 240, 922
Alw26I GTCTC 7 cut(s) 102, 152, 313, 538, 597, 1433, 1606
AlwI GGATC 1 cut(s) 1308
Ama87I CYCGRG 2 cut(s) 887, 1132
Aor13HI TCCGGA 2 cut(s) 872, 945
AoxI GGCC 3 cut(s) 1418, 1478, 1643
ApeKI GCWGC 4 cut(s) 68, 461, 740, 1525
ApoI RAATTY 1 cut(s) 1039
ArsI GACNNNNNNTTYG 4 cut(s) 506, 538, 854, 886
AscI GGCGCGCC 1 cut(s) 447
AspLEI GCGC 7 cut(s) 266, 324, 449, 451, 461, 838, 1022
AspS9I GGNCC 5 cut(s) 496, 582, 690, 753, 948
AsuC2I CCSGG 4 cut(s) 896, 1127, 1388, 1588
AsuHPI GGTGA 3 cut(s) 982, 1277, 1486
AvaI CYCGRG 2 cut(s) 887, 1132
AvaII GGWCC 5 cut(s) 496, 582, 690, 753, 948
BaeGI GKGCMC 1 cut(s) 255
BanI GGYRCC 2 cut(s) 263, 1540
BanII GRGCYC 2 cut(s) 240, 922
BauI CACGAG 1 cut(s) 500
BbsI GAAGAC 2 cut(s) 337, 619
Bbv12I GWGCWC 2 cut(s) 240, 922
BbvI GCAGC 4 cut(s) 80, 473, 752, 1512
BccI CCATC 3 cut(s) 275, 392, 1612
BcgI CGANNNNNNTGC 2 cut(s) 1147, 1181
BciT130I CCWGG 2 cut(s) 293, 959
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 4 cut(s) 896, 1127, 1388, 1588
BcoDI GTCTC 7 cut(s) 102, 152, 313, 538, 597, 1433, 1606
BfaI CTAG 2 cut(s) 1202, 1647
BfoI RGCGCY 1 cut(s) 267
BglII AGATCT 1 cut(s) 7
BisI GCNGC 7 cut(s) 69, 301, 462, 741, 926, 1404, 1526
BlsI GCNGC 7 cut(s) 70, 302, 463, 742, 927, 1405, 1527
Bme1390I CCNGG 6 cut(s) 293, 896, 959, 1127, 1388, 1588
Bme18I GGWCC 5 cut(s) 496, 582, 690, 753, 948
BmeRI GACNNNNNGTC 3 cut(s) 91, 138, 1159
BmeT110I CYCGRG 2 cut(s) 887, 1132
BmgBI CACGTC 5 cut(s) 21, 576, 1117, 1161, 1270
BmgT120I GGNCC 5 cut(s) 496, 582, 690, 753, 948
BmiI GGNNCC 8 cut(s) 265, 555, 583, 692, 754, 877, 933, 1542
BmrFI CCNGG 6 cut(s) 293, 896, 959, 1127, 1388, 1588
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 3 cut(s) 1188, 1269, 1291
BmuI ACTGGG 1 cut(s) 667
BpiI GAAGAC 2 cut(s) 337, 619
BplI GAGNNNNNCTC 2 cut(s) 518, 550
BpuMI CCSGG 4 cut(s) 896, 1127, 1388, 1588
BsaAI YACGTR 1 cut(s) 489
BsaHI GRCGYC 3 cut(s) 264, 780, 1442
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 6 cut(s) 48, 175, 597, 957, 958, 1317
BsaWI WCCGGW 3 cut(s) 872, 934, 945
BsaXI ACNNNNNCTCC 8 cut(s) 395, 425, 577, 607, 1352, 1382, 1430, 1460
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 260, 621, 622, 750, 1132, 1133
Bse118I RCCGGY 4 cut(s) 676, 838, 928, 1048
Bse1I ACTGG 1 cut(s) 662
BseAI TCCGGA 2 cut(s) 872, 945
BseBI CCWGG 2 cut(s) 293, 959
BseDI CCNNGG 6 cut(s) 48, 175, 597, 957, 958, 1317
BseGI GGATG 6 cut(s) 32, 170, 219, 267, 903, 1282
BseLI CCNNNNNNNGG 7 cut(s) 123, 260, 621, 622, 750, 1132, 1133
BseMII CTCAG 4 cut(s) 1047, 1153, 1340, 1601
BseNI ACTGG 1 cut(s) 662
BsePI GCGCGC 1 cut(s) 447
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 4 cut(s) 80, 473, 752, 1512
BseYI CCCAGC 1 cut(s) 223
Bsh1236I CGCG 5 cut(s) 153, 449, 459, 1022, 1152
Bsh1285I CGRYCG 2 cut(s) 1098, 1587
BshFI GGCC 3 cut(s) 1420, 1480, 1645
BshNI GGYRCC 2 cut(s) 263, 1540
BsiEI CGRYCG 2 cut(s) 1098, 1587
BsiHKAI GWGCWC 2 cut(s) 240, 922
BsiHKCI CYCGRG 2 cut(s) 887, 1132
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 5 cut(s) 595, 766, 851, 940, 1149
BslI CCNNNNNNNGG 7 cut(s) 123, 260, 621, 622, 750, 1132, 1133
BsmAI GTCTC 7 cut(s) 102, 152, 313, 538, 597, 1433, 1606
BsmBI CGTCTC 3 cut(s) 313, 597, 1433
BsmFI GGGAC 5 cut(s) 595, 766, 851, 940, 1149
BsmI GAATGC 1 cut(s) 1451
BsnI GGCC 3 cut(s) 1420, 1480, 1645
Bso31I GGTCTC 1 cut(s) 538
BsoBI CYCGRG 2 cut(s) 887, 1132
Bsp1286I GDGCHC 3 cut(s) 240, 255, 922
Bsp13I TCCGGA 2 cut(s) 872, 945
Bsp143I GATC 5 cut(s) 7, 651, 801, 1313, 1500
BspANI GGCC 3 cut(s) 1420, 1480, 1645
BspCNI CTCAG 4 cut(s) 1048, 1152, 1341, 1602
BspEI TCCGGA 2 cut(s) 872, 945
BspFNI CGCG 5 cut(s) 153, 449, 459, 1022, 1152
BspLI GGNNCC 8 cut(s) 265, 555, 583, 692, 754, 877, 933, 1542
BspPI GGATC 1 cut(s) 1308
BspT107I GGYRCC 2 cut(s) 263, 1540
BspTNI GGTCTC 1 cut(s) 538
BsrFI RCCGGY 4 cut(s) 676, 838, 928, 1048
BsrI ACTGG 1 cut(s) 662
BssAI RCCGGY 4 cut(s) 676, 838, 928, 1048
BssECI CCNNGG 6 cut(s) 48, 175, 597, 957, 958, 1317
BssHII GCGCGC 1 cut(s) 447
BssMI GATC 5 cut(s) 7, 651, 801, 1313, 1500
BssNAI GTATAC 1 cut(s) 1380
BssNI GRCGYC 3 cut(s) 264, 780, 1442
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 4 cut(s) 48, 175, 597, 1317
Bst1107I GTATAC 1 cut(s) 1380
Bst2BI CACGAG 1 cut(s) 500
Bst2UI CCWGG 2 cut(s) 293, 959
Bst4CI ACNGT 5 cut(s) 76, 140, 307, 1096, 1552
Bst6I CTCTTC 3 cut(s) 398, 1022, 1460
BstACI GRCGYC 3 cut(s) 264, 780, 1442
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 449, 930, 1050
BstDEI CTNAG 4 cut(s) 1056, 1139, 1349, 1610
BstF5I GGATG 6 cut(s) 32, 170, 219, 267, 903, 1282
BstFNI CGCG 5 cut(s) 153, 449, 459, 1022, 1152
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 7 cut(s) 266, 324, 449, 451, 461, 838, 1022
BstKTI GATC 5 cut(s) 10, 654, 804, 1316, 1503
BstMAI GTCTC 7 cut(s) 102, 152, 313, 538, 597, 1433, 1606
BstMBI GATC 5 cut(s) 7, 651, 801, 1313, 1500
BstMCI CGRYCG 2 cut(s) 1098, 1587
BstMWI GCNNNNNNNGC 5 cut(s) 297, 844, 853, 1149, 1412
BstNI CCWGG 2 cut(s) 293, 959
BstNSI RCATGY 2 cut(s) 829, 856
BstSCI CCNGG 6 cut(s) 291, 894, 957, 1125, 1386, 1586
BstSLI GKGCMC 1 cut(s) 255
BstUI CGCG 5 cut(s) 153, 449, 459, 1022, 1152
BstV1I GCAGC 4 cut(s) 80, 473, 752, 1512
BstV2I GAAGAC 2 cut(s) 337, 619
BstX2I RGATCY 2 cut(s) 7, 1313
BstYI RGATCY 2 cut(s) 7, 1313
BstZ17I GTATAC 1 cut(s) 1380
BsuRI GGCC 3 cut(s) 1420, 1480, 1645
BtgZI GCGATG 2 cut(s) 415, 619
BtrI CACGTC 5 cut(s) 21, 576, 1117, 1161, 1270
BtsCI GGATG 6 cut(s) 32, 170, 219, 267, 903, 1282
BtsI GCAGTG 1 cut(s) 1360
BtsIMutI CAGTG 1 cut(s) 1360
Cac8I GCNNGC 3 cut(s) 449, 930, 1050
CfoI GCGC 7 cut(s) 266, 324, 449, 451, 461, 838, 1022
Cfr10I RCCGGY 4 cut(s) 676, 838, 928, 1048
Cfr13I GGNCC 5 cut(s) 496, 582, 690, 753, 948
CpoI CGGWCCG 1 cut(s) 948
CseI GACGC 4 cut(s) 571, 754, 788, 1450
Csp6I GTAC 6 cut(s) 232, 314, 490, 665, 682, 892
CspCI CAANNNNNGTGG 2 cut(s) 1533, 1568
CspI CGGWCCG 1 cut(s) 948
CviAII CATG 7 cut(s) 229, 256, 799, 826, 853, 1240, 1537
CviQI GTAC 6 cut(s) 232, 314, 490, 665, 682, 892
DdeI CTNAG 4 cut(s) 1056, 1139, 1349, 1610
DinI GGCGCC 1 cut(s) 265
DpnI GATC 5 cut(s) 9, 653, 803, 1315, 1502
DpnII GATC 5 cut(s) 7, 651, 801, 1313, 1500
DrdI GACNNNNNNGTC 1 cut(s) 589
DriI GACNNNNNGTC 3 cut(s) 91, 138, 1159
DseDI GACNNNNNNGTC 1 cut(s) 589
Eam1104I CTCTTC 3 cut(s) 398, 1022, 1460
Eam1105I GACNNNNNGTC 3 cut(s) 91, 138, 1159
EarI CTCTTC 3 cut(s) 398, 1022, 1460
EciI GGCGGA 1 cut(s) 912
Ecl136II GAGCTC 2 cut(s) 238, 920
Eco130I CCWWGG 4 cut(s) 48, 175, 597, 1317
Eco147I AGGCCT 1 cut(s) 1645
Eco24I GRGCYC 2 cut(s) 240, 922
Eco31I GGTCTC 1 cut(s) 538
Eco47I GGWCC 5 cut(s) 496, 582, 690, 753, 948
Eco53kI GAGCTC 2 cut(s) 238, 920
Eco57I CTGAAG 2 cut(s) 32, 1091
Eco88I CYCGRG 2 cut(s) 887, 1132
EcoICRI GAGCTC 2 cut(s) 238, 920
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 2 cut(s) 291, 957
EcoT14I CCWWGG 4 cut(s) 48, 175, 597, 1317
EcoT22I ATGCAT 1 cut(s) 1284
EcoT38I GRGCYC 2 cut(s) 240, 922
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 4 cut(s) 48, 175, 597, 1317
Esp3I CGTCTC 3 cut(s) 313, 597, 1433
FaeI CATG 7 cut(s) 232, 259, 802, 829, 856, 1243, 1540
FaqI GGGAC 5 cut(s) 595, 766, 851, 940, 1149
FatI CATG 7 cut(s) 228, 255, 798, 825, 852, 1239, 1536
FauI CCCGC 4 cut(s) 296, 994, 1181, 1328
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 3 cut(s) 78, 351, 1379
Fnu4HI GCNGC 7 cut(s) 69, 301, 462, 741, 926, 1404, 1526
FokI GGATG 6 cut(s) 19, 157, 206, 254, 890, 1269
FriOI GRGCYC 2 cut(s) 240, 922
Fsp4HI GCNGC 7 cut(s) 69, 301, 462, 741, 926, 1404, 1526
FspBI CTAG 2 cut(s) 1202, 1647
GlaI GCGC 7 cut(s) 265, 323, 448, 450, 460, 837, 1021
GluI GCNGC 7 cut(s) 69, 301, 462, 741, 926, 1404, 1526
GsaI CCCAGC 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 3 cut(s) 1420, 1480, 1645
HgaI GACGC 4 cut(s) 571, 754, 788, 1450
HhaI GCGC 7 cut(s) 266, 324, 449, 451, 461, 838, 1022
Hin1I GRCGYC 3 cut(s) 264, 780, 1442
Hin1II CATG 7 cut(s) 232, 259, 802, 829, 856, 1243, 1540
Hin6I GCGC 7 cut(s) 264, 322, 447, 449, 459, 836, 1020
HinP1I GCGC 7 cut(s) 264, 322, 447, 449, 459, 836, 1020
HincII GTYRAC 2 cut(s) 352, 1396
HindII GTYRAC 2 cut(s) 352, 1396
HphI GGTGA 3 cut(s) 982, 1277, 1486
Hpy188I TCNGA 4 cut(s) 12, 558, 1350, 1599
HpyAV CCTTC 2 cut(s) 1489, 1618
HpyCH4III ACNGT 5 cut(s) 76, 140, 307, 1096, 1552
HpyCH4IV ACGT 9 cut(s) 20, 195, 488, 575, 864, 1116, 1160, 1269, 1323
HpyCH4V TGCA 6 cut(s) 364, 486, 1239, 1282, 1451, 1515
HpyF10VI GCNNNNNNNGC 5 cut(s) 297, 844, 853, 1149, 1412
HpyF3I CTNAG 4 cut(s) 1056, 1139, 1349, 1610
HpySE526I ACGT 9 cut(s) 20, 195, 488, 575, 864, 1116, 1160, 1269, 1323
Hsp92I GRCGYC 3 cut(s) 264, 780, 1442
Hsp92II CATG 7 cut(s) 232, 259, 802, 829, 856, 1243, 1540
HspAI GCGC 7 cut(s) 264, 322, 447, 449, 459, 836, 1020
KasI GGCGCC 1 cut(s) 263
Kpn2I TCCGGA 2 cut(s) 872, 945
KroI GCCGGC 2 cut(s) 928, 1048
KroNI GCCGGC 2 cut(s) 930, 1050
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 5 cut(s) 7, 651, 801, 1313, 1500
LmnI GCTCC 5 cut(s) 243, 720, 917, 925, 937
Lsp1109I GCAGC 4 cut(s) 80, 473, 752, 1512
LweI GCATC 3 cut(s) 1188, 1269, 1291
MaeI CTAG 2 cut(s) 1202, 1647
MaeII ACGT 9 cut(s) 20, 195, 488, 575, 864, 1116, 1160, 1269, 1323
MaeIII GTNAC 4 cut(s) 453, 817, 974, 1265
MalI GATC 5 cut(s) 9, 653, 803, 1315, 1502
MboI GATC 5 cut(s) 7, 651, 801, 1313, 1500
MboII GAAGA 8 cut(s) 17, 337, 385, 619, 1039, 1477, 1531, 1603
MflI RGATCY 2 cut(s) 7, 1313
MhlI GDGCHC 3 cut(s) 240, 255, 922
MluCI AATT 4 cut(s) 359, 568, 1039, 1302
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 8 cut(s) 341, 414, 612, 962, 982, 1124, 1131, 1376
Mph1103I ATGCAT 1 cut(s) 1284
MroI TCCGGA 2 cut(s) 872, 945
MroNI GCCGGC 2 cut(s) 928, 1048
MseI TTAA 1 cut(s) 1305
MslI CAYNNNNRTG 1 cut(s) 981
MspR9I CCNGG 6 cut(s) 293, 896, 959, 1127, 1388, 1588
Mva1269I GAATGC 1 cut(s) 1451
MvaI CCWGG 2 cut(s) 293, 959
MvnI CGCG 5 cut(s) 153, 449, 459, 1022, 1152
MwoI GCNNNNNNNGC 5 cut(s) 297, 844, 853, 1149, 1412
NaeI GCCGGC 2 cut(s) 930, 1050
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 4 cut(s) 896, 1127, 1388, 1588
NdeII GATC 5 cut(s) 7, 651, 801, 1313, 1500
NgoMIV GCCGGC 2 cut(s) 928, 1048
NlaIII CATG 7 cut(s) 232, 259, 802, 829, 856, 1243, 1540
NlaIV GGNNCC 8 cut(s) 265, 555, 583, 692, 754, 877, 933, 1542
NmeAIII GCCGAG 1 cut(s) 1146
NmuCI GTSAC 3 cut(s) 453, 974, 1265
NsiI ATGCAT 1 cut(s) 1284
NspI RCATGY 2 cut(s) 829, 856
PaeR7I CTCGAG 1 cut(s) 887
PalAI GGCGCGCC 1 cut(s) 447
PasI CCCWGGG 1 cut(s) 958
PauI GCGCGC 1 cut(s) 447
PceI AGGCCT 1 cut(s) 1645
PciI ACATGT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 6 cut(s) 201, 348, 354, 498, 522, 1173
PctI GAATGC 1 cut(s) 1451
PdiI GCCGGC 2 cut(s) 930, 1050
PfeI GAWTC 4 cut(s) 440, 644, 1345, 1594
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 1 cut(s) 952
PkrI GCNGC 7 cut(s) 70, 302, 463, 742, 927, 1405, 1527
PleI GAGTC 8 cut(s) 340, 414, 611, 961, 981, 1124, 1131, 1375
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 8 cut(s) 340, 414, 611, 961, 981, 1124, 1131, 1375
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 496
PscI ACATGT 1 cut(s) 825
PsiI TTATAA 1 cut(s) 1331
Psp124BI GAGCTC 2 cut(s) 240, 922
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 2 cut(s) 291, 957
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 2 cut(s) 291, 957
PspLI CGTACG 1 cut(s) 489
PspN4I GGNNCC 8 cut(s) 265, 555, 583, 692, 754, 877, 933, 1542
PspPI GGNCC 5 cut(s) 496, 582, 690, 753, 948
PspPPI RGGWCCY 1 cut(s) 496
PspXI VCTCGAGB 1 cut(s) 887
PsuI RGATCY 2 cut(s) 7, 1313
PsyI GACNNNGTC 1 cut(s) 952
PteI GCGCGC 1 cut(s) 447
RsaI GTAC 6 cut(s) 233, 315, 491, 666, 683, 893
RsaNI GTAC 6 cut(s) 232, 314, 490, 665, 682, 892
RseI CAYNNNNRTG 1 cut(s) 981
Rsr2I CGGWCCG 1 cut(s) 948
RsrII CGGWCCG 1 cut(s) 948
SacI GAGCTC 2 cut(s) 240, 922
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 1305
SatI GCNGC 7 cut(s) 69, 301, 462, 741, 926, 1404, 1526
Sau3AI GATC 5 cut(s) 7, 651, 801, 1313, 1500
Sau96I GGNCC 5 cut(s) 496, 582, 690, 753, 948
SchI GAGTC 8 cut(s) 341, 414, 612, 962, 982, 1124, 1131, 1376
ScrFI CCNGG 6 cut(s) 293, 896, 959, 1127, 1388, 1588
SduI GDGCHC 3 cut(s) 240, 255, 922
SfaNI GCATC 3 cut(s) 1188, 1269, 1291
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 887
SgrAI CRCCGGYG 1 cut(s) 838
SgsI GGCGCGCC 1 cut(s) 447
SinI GGWCC 5 cut(s) 496, 582, 690, 753, 948
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse9I AATT 4 cut(s) 359, 568, 1039, 1302
SseBI AGGCCT 1 cut(s) 1645
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 1202, 1647
SstI GAGCTC 2 cut(s) 240, 922
StuI AGGCCT 1 cut(s) 1645
StyD4I CCNGG 6 cut(s) 291, 894, 957, 1125, 1386, 1586
StyI CCWWGG 4 cut(s) 48, 175, 597, 1317
TaaI ACNGT 5 cut(s) 76, 140, 307, 1096, 1552
TaiI ACGT 9 cut(s) 23, 198, 491, 578, 867, 1119, 1163, 1272, 1326
TaqII GACCGA 2 cut(s) 965, 1007
TasI AATT 4 cut(s) 359, 568, 1039, 1302
TauI GCSGC 3 cut(s) 303, 928, 1406
TfiI GAWTC 4 cut(s) 440, 644, 1345, 1594
Tru1I TTAA 1 cut(s) 1305
Tru9I TTAA 1 cut(s) 1305
TscAI CASTG 1 cut(s) 1360
TseFI GTSAC 3 cut(s) 453, 974, 1265
TseI GCWGC 4 cut(s) 68, 461, 740, 1525
Tsp45I GTSAC 3 cut(s) 453, 974, 1265
TspDTI ATGAA 2 cut(s) 619, 1256
TspGWI ACGGA 8 cut(s) 85, 120, 193, 301, 747, 758, 1505, 1577
TspRI CASTG 1 cut(s) 1360
Tth111I GACNNNGTC 1 cut(s) 952
VpaK11BI GGWCC 5 cut(s) 496, 582, 690, 753, 948
XapI RAATTY 1 cut(s) 1039
XceI RCATGY 2 cut(s) 829, 856
XhoI CTCGAG 1 cut(s) 887
XmiI GTMKAC 3 cut(s) 78, 351, 1379
XspI CTAG 2 cut(s) 1202, 1647
Zsp2I ATGCAT 1 cut(s) 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.