RLG00000023003

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000677
Physical Location & Seq
Forward (+)
16551025 .. 16552960
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023003

Sequence Viewer

Length: 1662 bp
ATGGACCAGTTGAAACCTAGTCCTGCAAACCTCTGCCCTCAAACTCCCCTGGACTTCTTAGACCGAGCAGCGATCGTCTACGGTGACTGGCCCTCCATCATCTACAACTCCACCACCACCTACACCTGGTCCCAAACCCACTGTCGATGTCTACAGCTGGCTTCCTCCATCTCCACCCTCGTCAAGAAATCCCAGGTCGTCTCCGTGGTGTCCCCCAACATTCCCGCCATGTACGAACTCCACTTTGCTGTCCCCATGTTGGGAGCCGTCCTCAACAACATCAACACCCGCCTCGACGCCCACACCGTCTCCGTGCTCCTCCAACACAGCGATTCCAAGCTCGTCTTCGTCGACTTCCTCTCCCTCAACCTCGTCCTCCAAGCCCTCTCCTTGTTCCCCTCTCACATCTCCACCCCACCTCTCGTCCTCATCACTGATCATCAACCTCCCTCAACCTCCACCTTTGATTTCATAGCGGACACGTACGACAACATGGTGGCCAAAGGCGATCCCGGGTTCCTTTGGGTCCGGCCGGCGAGCGAGTGGGACCCAATAGTGCTGAACTACACGTCAGGGACGACGGCAGCTCCGAAAGGCGCGGTACACTGCCACAGAGCCGTCTTCAACGTGAGTCTCGTTTCAATCATAGACTGGTCAGTTCCTAAAAATTGTGTGTACCTTTGGACCCTACCGATGTTCCATGCAAACGGGTGGTGCTACCCGTGGGCCATAGCCGCTGTGGGTGGGACCAACATCTGCCTGCGCAAGTTCGACGGTCCTATAATCTACGGTCTGATCCAGAAACACAGAGTGACTCACATGTGCGCCGCGCCTGTCGTTCTCAACATGCTGGTCAACCACCCAAGAACCGACCCGCTTCCGAACCCGGTTTGTATCTTAACCGCCGGAGCTCCTCCTCCTTCGAGTTTGCTGCTCCGGGCCGAGTCACTCGGGTTTGTTGTGAGTCACGGTTACGGGTTGACTGAAACAGCCGGGGTGGCGGTGTCGTGTGCCTGGAAGCCGGAGTGGAATAAGTTTCCGGCGACGGAGAAGGCGAGGTTGAAGGCGAGGCAAGGAGTGAGGACGGTTGGAATGACCGACGCGGATGTGGTGGATCCGGATTCGGGTTTGAGCGTGAAACAAAACGGGTCGGATGTTGGAGAAATAGTTATGCGAGGCGGGTGCGTCATGTTGGGTTACCTCAAAGACCCGGAAACGACGAGCAAAACCGTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTCGAGTCAGTTCTCTACACCAACGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTCAGCCTGAAAGCTGACGTCAGGCCGAGGCCGACGGAGAAGGAGATAATAGAGTTCTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACAGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTCTACGCTGAGAGAGATTGCGAAGAATATTGGGTCTACGTCAACGACGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

554

Amino Acids

60.43

Weight (kDa)

7.19

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 401 6.4e-70 AMP-binding enzyme
AMP-binding_C PF13193 452 - 529 6.9e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 1266, 1470, 1577
Acc16I TGCGCA 1 cut(s) 764
AccB1I GGYRCC 1 cut(s) 1534
AccI GTMKAC 6 cut(s) 78, 151, 351, 1577, 1598, 1631
AccII CGCG 5 cut(s) 599, 830, 1103, 1385, 1445
AccIII TCCGGA 1 cut(s) 1117
AclWI GGATC 6 cut(s) 503, 790, 1109, 1122, 1302, 1311
AcoI YGGCCR 3 cut(s) 498, 530, 1398
AcsI RAATTY 1 cut(s) 1525
AcyI GRCGYC 5 cut(s) 297, 1263, 1436, 1467, 1574
AdeI CACNNNGTG 1 cut(s) 811
AfaI GTAC 4 cut(s) 233, 485, 603, 677
AfiI CCNNNNNNNGG 4 cut(s) 126, 259, 260, 1124
AflII CTTAAG 1 cut(s) 1342
AflIII ACRYGT 3 cut(s) 480, 567, 819
AgsI TTSAA 5 cut(s) 13, 625, 642, 1063, 1555
AjiI CACGTC 2 cut(s) 570, 1318
AjnI CCWGG 4 cut(s) 48, 125, 192, 1013
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 7 cut(s) 157, 340, 587, 911, 1463, 1519, 1564
AluI AGCT 7 cut(s) 157, 340, 587, 911, 1463, 1519, 1564
Alw21I GWGCWC 2 cut(s) 318, 913
Alw26I GTCTC 4 cut(s) 205, 313, 638, 1427
AlwI GGATC 6 cut(s) 503, 790, 1109, 1122, 1302, 1311
Ama87I CYCGRG 2 cut(s) 512, 950
Aor13HI TCCGGA 1 cut(s) 1117
AoxI GGCC 8 cut(s) 89, 498, 530, 726, 939, 1398, 1472, 1478
ApeKI GCWGC 6 cut(s) 68, 584, 931, 1347, 1519, 1564
ApoI RAATTY 1 cut(s) 1525
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspLEI GCGC 5 cut(s) 599, 765, 827, 832, 1445
AsuC2I CCSGG 7 cut(s) 513, 514, 887, 938, 994, 1211, 1280
AsuHPI GGTGA 2 cut(s) 95, 1399
AvaI CYCGRG 2 cut(s) 512, 950
AvaII GGWCC 8 cut(s) 4, 129, 526, 547, 684, 747, 776, 1647
BalI TGGCCA 1 cut(s) 500
BamHI GGATCC 1 cut(s) 1114
BanI GGYRCC 1 cut(s) 1534
BanII GRGCYC 1 cut(s) 913
BbsI GAAGAC 4 cut(s) 337, 613, 1547, 1577
Bbv12I GWGCWC 2 cut(s) 318, 913
BbvI GCAGC 6 cut(s) 80, 596, 918, 1359, 1506, 1551
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 5 cut(s) 251, 597, 602, 1385, 1423
BcgI CGANNNNNNTGC 4 cut(s) 913, 947, 1164, 1198
BciT130I CCWGG 4 cut(s) 50, 127, 194, 1015
BclI TGATCA 2 cut(s) 436, 1320
BcnI CCSGG 7 cut(s) 513, 514, 887, 938, 994, 1211, 1280
BcoDI GTCTC 4 cut(s) 205, 313, 638, 1427
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 2 cut(s) 152, 1507
BfrI CTTAAG 1 cut(s) 1342
BglI GCCNNNNNGGC 2 cut(s) 998, 1406
BlpI GCTNAGC 1 cut(s) 1410
Bme18I GGWCC 8 cut(s) 4, 129, 526, 547, 684, 747, 776, 1647
BmeT110I CYCGRG 2 cut(s) 512, 950
BmgBI CACGTC 2 cut(s) 570, 1318
BpiI GAAGAC 4 cut(s) 337, 613, 1547, 1577
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 1 cut(s) 1313
Bpu1102I GCTNAGC 1 cut(s) 1410
BpuMI CCSGG 7 cut(s) 513, 514, 887, 938, 994, 1211, 1280
Bsa29I ATCGAT 1 cut(s) 1306
BsaAI YACGTR 1 cut(s) 483
BsaHI GRCGYC 5 cut(s) 297, 1263, 1436, 1467, 1574
BsaWI WCCGGW 1 cut(s) 1117
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 259, 260, 1124
Bse118I RCCGGY 2 cut(s) 532, 1256
Bse1I ACTGG 4 cut(s) 7, 92, 656, 1650
BseAI TCCGGA 1 cut(s) 1117
BseBI CCWGG 4 cut(s) 50, 127, 194, 1015
BseCI ATCGAT 1 cut(s) 1306
BseGI GGATG 2 cut(s) 1111, 1159
BseLI CCNNNNNNNGG 4 cut(s) 126, 259, 260, 1124
BseMII CTCAG 1 cut(s) 1595
BseNI ACTGG 4 cut(s) 7, 92, 656, 1650
BseRI GAGGAG 3 cut(s) 308, 903, 906
BseX3I CGGCCG 2 cut(s) 530, 1398
BseXI GCAGC 6 cut(s) 80, 596, 918, 1359, 1506, 1551
Bsh1236I CGCG 5 cut(s) 599, 830, 1103, 1385, 1445
Bsh1285I CGRYCG 3 cut(s) 75, 533, 1401
BshFI GGCC 8 cut(s) 91, 500, 532, 728, 941, 1400, 1474, 1480
BshNI GGYRCC 1 cut(s) 1534
BshVI ATCGAT 1 cut(s) 1306
BsiEI CGRYCG 3 cut(s) 75, 533, 1401
BsiHKAI GWGCWC 2 cut(s) 318, 913
BsiHKCI CYCGRG 2 cut(s) 512, 950
BsiWI CGTACG 1 cut(s) 483
BslFI GGGAC 6 cut(s) 115, 196, 236, 560, 589, 760
BslI CCNNNNNNNGG 4 cut(s) 126, 259, 260, 1124
BsmAI GTCTC 4 cut(s) 205, 313, 638, 1427
BsmBI CGTCTC 3 cut(s) 205, 313, 1427
BsmFI GGGAC 6 cut(s) 115, 196, 236, 560, 589, 760
BsnI GGCC 8 cut(s) 91, 500, 532, 728, 941, 1400, 1474, 1480
BsoBI CYCGRG 2 cut(s) 512, 950
Bsp1286I GDGCHC 2 cut(s) 318, 913
Bsp13I TCCGGA 1 cut(s) 1117
Bsp143I GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
Bsp1720I GCTNAGC 1 cut(s) 1410
BspANI GGCC 8 cut(s) 91, 500, 532, 728, 941, 1400, 1474, 1480
BspCNI CTCAG 1 cut(s) 1596
BspDI ATCGAT 1 cut(s) 1306
BspEI TCCGGA 1 cut(s) 1117
BspFNI CGCG 5 cut(s) 599, 830, 1103, 1385, 1445
BspMAI CTGCAG 1 cut(s) 1511
BspPI GGATC 6 cut(s) 503, 790, 1109, 1122, 1302, 1311
BspT107I GGYRCC 1 cut(s) 1534
BspTI CTTAAG 1 cut(s) 1342
BsrFI RCCGGY 2 cut(s) 532, 1256
BsrI ACTGG 4 cut(s) 7, 92, 656, 1650
BssAI RCCGGY 2 cut(s) 532, 1256
BssMI GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
BssNI GRCGYC 5 cut(s) 297, 1263, 1436, 1467, 1574
BssT1I CCWWGG 1 cut(s) 1311
Bst2UI CCWGG 4 cut(s) 50, 127, 194, 1015
Bst4CI ACNGT 9 cut(s) 83, 143, 307, 776, 791, 971, 1087, 1231, 1546
BstACI GRCGYC 5 cut(s) 297, 1263, 1436, 1467, 1574
BstAFI CTTAAG 1 cut(s) 1342
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 4 cut(s) 159, 534, 538, 761
BstDEI CTNAG 3 cut(s) 58, 1410, 1604
BstDSI CCRYGG 4 cut(s) 204, 722, 1383, 1401
BstEII GGTNACC 1 cut(s) 1196
BstF5I GGATG 2 cut(s) 1111, 1159
BstFNI CGCG 5 cut(s) 599, 830, 1103, 1385, 1445
BstHHI GCGC 5 cut(s) 599, 765, 827, 832, 1445
BstKTI GATC 8 cut(s) 75, 439, 511, 798, 1117, 1306, 1310, 1323
BstMAI GTCTC 4 cut(s) 205, 313, 638, 1427
BstMBI GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
BstMCI CGRYCG 3 cut(s) 75, 533, 1401
BstMWI GCNNNNNNNGC 3 cut(s) 734, 998, 1406
BstNI CCWGG 4 cut(s) 50, 127, 194, 1015
BstNSI RCATGY 2 cut(s) 823, 850
BstPI GGTNACC 1 cut(s) 1196
BstSFI CTRYAG 2 cut(s) 152, 1507
BstUI CGCG 5 cut(s) 599, 830, 1103, 1385, 1445
BstV1I GCAGC 6 cut(s) 80, 596, 918, 1359, 1506, 1551
BstV2I GAAGAC 4 cut(s) 337, 613, 1547, 1577
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
BstZI CGGCCG 2 cut(s) 530, 1398
Bsu15I ATCGAT 1 cut(s) 1306
BsuRI GGCC 8 cut(s) 91, 500, 532, 728, 941, 1400, 1474, 1480
BsuTUI ATCGAT 1 cut(s) 1306
BtgI CCRYGG 4 cut(s) 204, 722, 1383, 1401
BtrI CACGTC 2 cut(s) 570, 1318
BtsCI GGATG 2 cut(s) 1111, 1159
BtsI GCAGTG 1 cut(s) 604
BtsIMutI CAGTG 4 cut(s) 139, 432, 604, 1551
Cac8I GCNNGC 4 cut(s) 159, 534, 538, 761
CfoI GCGC 5 cut(s) 599, 765, 827, 832, 1445
Cfr10I RCCGGY 2 cut(s) 532, 1256
Cfr42I CCGCGG 1 cut(s) 1386
Cfr9I CCCGGG 1 cut(s) 512
ClaI ATCGAT 1 cut(s) 1306
CseI GACGC 4 cut(s) 305, 1109, 1174, 1444
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 4 cut(s) 232, 484, 602, 676
CviAII CATG 8 cut(s) 229, 256, 493, 701, 820, 847, 1189, 1531
CviQI GTAC 4 cut(s) 232, 484, 602, 676
DdeI CTNAG 3 cut(s) 58, 1410, 1604
DpnI GATC 8 cut(s) 74, 438, 510, 797, 1116, 1305, 1309, 1322
DpnII GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
DraIII CACNNNGTG 1 cut(s) 811
EaeI YGGCCR 3 cut(s) 498, 530, 1398
EagI CGGCCG 2 cut(s) 530, 1398
Ecl136II GAGCTC 1 cut(s) 911
EclXI CGGCCG 2 cut(s) 530, 1398
Eco130I CCWWGG 1 cut(s) 1311
Eco24I GRGCYC 1 cut(s) 913
Eco47I GGWCC 8 cut(s) 4, 129, 526, 547, 684, 747, 776, 1647
Eco52I CGGCCG 2 cut(s) 530, 1398
Eco53kI GAGCTC 1 cut(s) 911
Eco88I CYCGRG 2 cut(s) 512, 950
Eco91I GGTNACC 1 cut(s) 1196
EcoICRI GAGCTC 1 cut(s) 911
EcoO109I RGGNCCY 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 1196
EcoRII CCWGG 4 cut(s) 48, 125, 192, 1013
EcoT14I CCWWGG 1 cut(s) 1311
EcoT38I GRGCYC 1 cut(s) 913
ErhI CCWWGG 1 cut(s) 1311
Esp3I CGTCTC 3 cut(s) 205, 313, 1427
FaeI CATG 8 cut(s) 232, 259, 496, 704, 823, 850, 1192, 1534
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 6 cut(s) 115, 196, 236, 560, 589, 760
FatI CATG 8 cut(s) 228, 255, 492, 700, 819, 846, 1188, 1530
FauI CCCGC 5 cut(s) 232, 296, 882, 1172, 1322
FbaI TGATCA 2 cut(s) 436, 1320
FblI GTMKAC 6 cut(s) 78, 151, 351, 1577, 1598, 1631
FokI GGATG 2 cut(s) 1118, 1166
FriOI GRGCYC 1 cut(s) 913
FspBI CTAG 1 cut(s) 18
FspI TGCGCA 1 cut(s) 764
GlaI GCGC 5 cut(s) 598, 764, 826, 831, 1444
GsuI CTGGAG 1 cut(s) 1313
HaeIII GGCC 8 cut(s) 91, 500, 532, 728, 941, 1400, 1474, 1480
HgaI GACGC 4 cut(s) 305, 1109, 1174, 1444
HhaI GCGC 5 cut(s) 599, 765, 827, 832, 1445
Hin1I GRCGYC 5 cut(s) 297, 1263, 1436, 1467, 1574
Hin1II CATG 8 cut(s) 232, 259, 496, 704, 823, 850, 1192, 1534
Hin6I GCGC 5 cut(s) 597, 763, 825, 830, 1443
HinP1I GCGC 5 cut(s) 597, 763, 825, 830, 1443
HincII GTYRAC 5 cut(s) 352, 856, 981, 1578, 1638
HindII GTYRAC 5 cut(s) 352, 856, 981, 1578, 1638
HinfI GANTC 8 cut(s) 332, 631, 814, 944, 964, 1121, 1361, 1588
HphI GGTGA 2 cut(s) 95, 1399
Hpy188I TCNGA 5 cut(s) 591, 795, 882, 1153, 1593
Hpy188III TCNNGA 4 cut(s) 184, 799, 1118, 1292
HpyAV CCTTC 4 cut(s) 930, 1045, 1057, 1483
HpyCH4III ACNGT 9 cut(s) 83, 143, 307, 776, 791, 971, 1087, 1231, 1546
HpyCH4IV ACGT 8 cut(s) 482, 569, 627, 1263, 1317, 1467, 1574, 1634
HpyCH4V TGCA 4 cut(s) 26, 704, 1276, 1509
HpyF10VI GCNNNNNNNGC 3 cut(s) 734, 998, 1406
HpyF3I CTNAG 3 cut(s) 58, 1410, 1604
HpySE526I ACGT 8 cut(s) 482, 569, 627, 1263, 1317, 1467, 1574, 1634
Hsp92I GRCGYC 5 cut(s) 297, 1263, 1436, 1467, 1574
Hsp92II CATG 8 cut(s) 232, 259, 496, 704, 823, 850, 1192, 1534
HspAI GCGC 5 cut(s) 597, 763, 825, 830, 1443
KflI GGGWCCC 1 cut(s) 547
Kpn2I TCCGGA 1 cut(s) 1117
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Ksp22I TGATCA 2 cut(s) 436, 1320
KspI CCGCGG 1 cut(s) 1386
Kzo9I GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
LmnI GCTCC 6 cut(s) 263, 321, 592, 908, 916, 939
Lsp1109I GCAGC 6 cut(s) 80, 596, 918, 1359, 1506, 1551
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 8 cut(s) 482, 569, 627, 1263, 1317, 1467, 1574, 1634
MaeIII GTNAC 6 cut(s) 83, 811, 945, 965, 971, 1196
MalI GATC 8 cut(s) 74, 438, 510, 797, 1116, 1305, 1309, 1322
MboI GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
MboII GAAGA 6 cut(s) 337, 613, 1552, 1582, 1597, 1630
MflI RGATCY 1 cut(s) 1114
MhlI GDGCHC 2 cut(s) 318, 913
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 2 cut(s) 667, 1525
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 5 cut(s) 640, 808, 953, 973, 1370
MmeI TCCRAC 4 cut(s) 346, 1069, 1131, 1138
Mox20I TGGCCA 1 cut(s) 500
MroI TCCGGA 1 cut(s) 1117
MroNI GCCGGC 1 cut(s) 532
MscI TGGCCA 1 cut(s) 500
MseI TTAA 2 cut(s) 899, 1343
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 3 cut(s) 157, 737, 1385
MspCI CTTAAG 1 cut(s) 1342
MteI GCGCNGCGC 1 cut(s) 828
MvaI CCWGG 4 cut(s) 50, 127, 194, 1015
MvnI CGCG 5 cut(s) 599, 830, 1103, 1385, 1445
MwoI GCNNNNNNNGC 3 cut(s) 734, 998, 1406
NaeI GCCGGC 1 cut(s) 534
NciI CCSGG 7 cut(s) 513, 514, 887, 938, 994, 1211, 1280
NdeII GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
NgoMIV GCCGGC 1 cut(s) 532
NlaIII CATG 8 cut(s) 232, 259, 496, 704, 823, 850, 1192, 1534
NmeAIII GCCGAG 2 cut(s) 967, 1500
NmuCI GTSAC 4 cut(s) 83, 811, 945, 965
NsbI TGCGCA 1 cut(s) 764
NspI RCATGY 2 cut(s) 823, 850
PciI ACATGT 1 cut(s) 819
PcsI WCGNNNNNNNCGW 9 cut(s) 303, 348, 575, 587, 633, 1052, 1398, 1442, 1640
PdiI GCCGGC 1 cut(s) 534
PfeI GAWTC 3 cut(s) 332, 1121, 1588
Pfl23II CGTACG 1 cut(s) 483
PflFI GACNNNGTC 1 cut(s) 1645
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 639, 808, 952, 972, 1369
PpsI GAGTC 5 cut(s) 639, 808, 952, 972, 1369
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 547
PscI ACATGT 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 913
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 4 cut(s) 48, 125, 192, 1013
PspEI GGTNACC 1 cut(s) 1196
PspGI CCWGG 4 cut(s) 48, 125, 192, 1013
PspLI CGTACG 1 cut(s) 483
PspPPI RGGWCCY 1 cut(s) 547
PstI CTGCAG 1 cut(s) 1511
PsuI RGATCY 1 cut(s) 1114
PsyI GACNNNGTC 1 cut(s) 1645
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 4 cut(s) 233, 485, 603, 677
RsaNI GTAC 4 cut(s) 232, 484, 602, 676
SacI GAGCTC 1 cut(s) 913
SacII CCGCGG 1 cut(s) 1386
SalI GTCGAC 2 cut(s) 350, 1576
SaqAI TTAA 2 cut(s) 899, 1343
Sau3AI GATC 8 cut(s) 72, 436, 508, 795, 1114, 1303, 1307, 1320
SchI GAGTC 5 cut(s) 640, 808, 953, 973, 1370
SduI GDGCHC 2 cut(s) 318, 913
SexAI ACCWGGT 1 cut(s) 125
SfcI CTRYAG 2 cut(s) 152, 1507
Sfr303I CCGCGG 1 cut(s) 1386
SgrAI CRCCGGYG 1 cut(s) 1256
SgrBI CCGCGG 1 cut(s) 1386
SgrDI CGTCGACG 1 cut(s) 1576
SinI GGWCC 8 cut(s) 4, 129, 526, 547, 684, 747, 776, 1647
SmaI CCCGGG 1 cut(s) 514
SmlI CTYRAG 1 cut(s) 1342
SmoI CTYRAG 1 cut(s) 1342
Sse9I AATT 2 cut(s) 667, 1525
SspI AATATT 1 cut(s) 1624
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 1 cut(s) 913
StyI CCWWGG 1 cut(s) 1311
TaaI ACNGT 9 cut(s) 83, 143, 307, 776, 791, 971, 1087, 1231, 1546
TaiI ACGT 8 cut(s) 485, 572, 630, 1266, 1320, 1470, 1577, 1637
TaqI TCGA 8 cut(s) 145, 294, 351, 771, 923, 1306, 1359, 1577
TaqII GACCGA 2 cut(s) 78, 1112
TasI AATT 2 cut(s) 667, 1525
TauI GCSGC 4 cut(s) 737, 830, 1385, 1400
TfiI GAWTC 3 cut(s) 332, 1121, 1588
Tru1I TTAA 2 cut(s) 899, 1343
Tru9I TTAA 2 cut(s) 899, 1343
TscAI CASTG 4 cut(s) 146, 439, 611, 1551
TseFI GTSAC 4 cut(s) 83, 811, 945, 965
TseI GCWGC 6 cut(s) 68, 584, 931, 1347, 1519, 1564
Tsp45I GTSAC 4 cut(s) 83, 811, 945, 965
TspDTI ATGAA 2 cut(s) 460, 1519
TspGWI ACGGA 4 cut(s) 193, 301, 1061, 1499
TspMI CCCGGG 1 cut(s) 512
TspRI CASTG 4 cut(s) 146, 439, 611, 1551
Tth111I GACNNNGTC 1 cut(s) 1645
Vha464I CTTAAG 1 cut(s) 1342
VpaK11BI GGWCC 8 cut(s) 4, 129, 526, 547, 684, 747, 776, 1647
XapI RAATTY 1 cut(s) 1525
XceI RCATGY 2 cut(s) 823, 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmaI CCCGGG 1 cut(s) 512
XmiI GTMKAC 6 cut(s) 78, 151, 351, 1577, 1598, 1631
XspI CTAG 1 cut(s) 18
ZraI GACGTC 3 cut(s) 1264, 1468, 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.