Rmu_ssc0000018.1_g000065

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000018.1
OG0000677
Physical Location & Seq
Reverse (-)
325283 .. 325702
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000018.1_g000065.1.cds

Sequence Viewer

Length: 420 bp
ctgcgccacgtacgaggaaatggtggagagcggcgacccggacttccagtgggccctgccggagaacgagggggacccgatgacgctgaactacacttccggcacgacttcctcgcccaagggagtggtccactgccacagagggctcttcatcatcaccgtcaactctctcctcgactgggccgtacccagacagcccgtctatctctggacgcttccgatgttccacgccaacgactggagctacccgtacgggatcccacgcttccgtttgtgagcctgaaagatgatgtggggcagaggccgacggagaaggagatgatagagtactgcagaggaaagctgccgaatttcatggtgccgaagacggtggtgtttattggggagctgccgaagacgtcgaaatttgtatgggattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

139

Amino Acids

15.61

Weight (kDa)

9.88

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 31
AciI CCGC 1 cut(s) 31
AclWI GGATC 2 cut(s) 251, 264
AcsI RAATTY 2 cut(s) 349, 404
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 4 cut(s) 12, 187, 252, 329
AfiI CCNNNNNNNGG 2 cut(s) 179, 238
AhdI GACNNNNNGTC 1 cut(s) 198
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
AluBI AGCT 3 cut(s) 244, 343, 388
AluI AGCT 3 cut(s) 244, 343, 388
AlwI GGATC 2 cut(s) 251, 264
AoxI GGCC 3 cut(s) 52, 181, 302
ApaI GGGCCC 1 cut(s) 56
ApeKI GCWGC 2 cut(s) 343, 388
ApoI RAATTY 2 cut(s) 349, 404
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 5 cut(s) 52, 53, 74, 128, 181
AsuC2I CCSGG 1 cut(s) 39
AsuHPI GGTGA 1 cut(s) 149
AvaII GGWCC 2 cut(s) 74, 128
BaeGI GKGCMC 1 cut(s) 56
BamHI GGATCC 1 cut(s) 256
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 2 cut(s) 56, 148
BbsI GAAGAC 2 cut(s) 371, 401
BbvI GCAGC 2 cut(s) 330, 375
BceAI ACGGC 1 cut(s) 168
BcnI CCSGG 1 cut(s) 39
BfmI CTRYAG 1 cut(s) 331
BisI GCNGC 3 cut(s) 32, 344, 389
BlsI GCNGC 3 cut(s) 33, 345, 390
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 2 cut(s) 74, 128
BmeRI GACNNNNNGTC 1 cut(s) 198
BmgT120I GGNCC 5 cut(s) 52, 53, 74, 128, 181
BmiI GGNNCC 5 cut(s) 54, 75, 76, 258, 360
BmrFI CCNGG 1 cut(s) 39
BmrI ACTGGG 1 cut(s) 188
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 2 cut(s) 371, 401
BpmI CTGGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 238
Bse1I ACTGG 3 cut(s) 47, 183, 243
BseDI CCNNGG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 179, 238
BseNI ACTGG 3 cut(s) 47, 183, 243
BseRI GAGGAG 1 cut(s) 162
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 2 cut(s) 330, 375
BshFI GGCC 3 cut(s) 54, 183, 304
BshNI GGYRCC 1 cut(s) 358
BsiSI CCGG 3 cut(s) 39, 60, 100
BsiWI CGTACG 2 cut(s) 10, 250
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 2 cut(s) 179, 238
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 3 cut(s) 54, 183, 304
Bsp120I GGGCCC 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 56, 148
Bsp143I GATC 1 cut(s) 256
BspACI CCGC 1 cut(s) 31
BspANI GGCC 3 cut(s) 54, 183, 304
BspLI GGNNCC 5 cut(s) 54, 75, 76, 258, 360
BspMAI CTGCAG 1 cut(s) 335
BspPI GGATC 2 cut(s) 251, 264
BspQI GCTCTTC 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 358
BsrBI CCGCTC 1 cut(s) 31
BsrI ACTGG 3 cut(s) 47, 183, 243
BssECI CCNNGG 1 cut(s) 118
BssMI GATC 1 cut(s) 256
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 161, 370
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 398
BstBAI YACGTR 1 cut(s) 10
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 259
BstMBI GATC 1 cut(s) 256
BstSCI CCNGG 1 cut(s) 37
BstSFI CTRYAG 1 cut(s) 331
BstSLI GKGCMC 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 330, 375
BstV2I GAAGAC 2 cut(s) 371, 401
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 125
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 3 cut(s) 54, 183, 304
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 2 cut(s) 54, 131
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 5 cut(s) 52, 53, 74, 128, 181
CseI GACGC 2 cut(s) 92, 221
Csp6I GTAC 4 cut(s) 11, 186, 251, 328
CviAII CATG 1 cut(s) 355
CviJI RGCY 9 cut(s) 54, 146, 183, 197, 244, 279, 304, 343, 388
CviKI_1 RGCY 9 cut(s) 54, 146, 183, 197, 244, 279, 304, 343, 388
CviQI GTAC 4 cut(s) 11, 186, 251, 328
DpnI GATC 1 cut(s) 258
DpnII GATC 1 cut(s) 256
DriI GACNNNNNGTC 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 153
Eam1105I GACNNNNNGTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 56, 148
Eco47I GGWCC 2 cut(s) 74, 128
EcoO109I RGGNCCY 2 cut(s) 53, 74
EcoT14I CCWWGG 1 cut(s) 118
EcoT38I GRGCYC 2 cut(s) 56, 148
ErhI CCWWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 358
FaiI YATR 2 cut(s) 356, 412
FaqI GGGAC 1 cut(s) 87
FatI CATG 1 cut(s) 354
Fnu4HI GCNGC 3 cut(s) 32, 344, 389
FriOI GRGCYC 2 cut(s) 56, 148
Fsp4HI GCNGC 3 cut(s) 32, 344, 389
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 32, 344, 389
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 3 cut(s) 54, 183, 304
HapII CCGG 3 cut(s) 39, 60, 100
HgaI GACGC 2 cut(s) 92, 221
HhaI GCGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 164
HindII GTYRAC 1 cut(s) 164
HpaII CCGG 3 cut(s) 39, 60, 100
HphI GGTGA 1 cut(s) 149
Hpy166II GTNNAC 2 cut(s) 131, 164
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 1 cut(s) 209
Hpy8I GTNNAC 2 cut(s) 131, 164
Hpy99I CGWCG 2 cut(s) 310, 403
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 2 cut(s) 161, 370
HpyCH4IV ACGT 2 cut(s) 9, 398
HpyCH4V TGCA 1 cut(s) 333
HpySE526I ACGT 2 cut(s) 9, 398
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 256
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 2 cut(s) 241, 385
Lsp1109I GCAGC 2 cut(s) 330, 375
MaeII ACGT 2 cut(s) 9, 398
MalI GATC 1 cut(s) 258
MbiI CCGCTC 1 cut(s) 31
MboI GATC 1 cut(s) 256
MboII GAAGA 3 cut(s) 140, 376, 406
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 56, 148
MluCI AATT 2 cut(s) 349, 404
MnlI CCTC 7 cut(s) 8, 62, 122, 135, 183, 294, 329
MspI CCGG 3 cut(s) 39, 60, 100
MspR9I CCNGG 1 cut(s) 39
NciI CCSGG 1 cut(s) 39
NdeII GATC 1 cut(s) 256
NlaIII CATG 1 cut(s) 358
NlaIV GGNNCC 5 cut(s) 54, 75, 76, 258, 360
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 181
Pfl23II CGTACG 2 cut(s) 10, 250
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 3 cut(s) 33, 345, 390
Ppu21I YACGTR 1 cut(s) 10
PpuMI RGGWCCY 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 74
PspLI CGTACG 2 cut(s) 10, 250
PspN4I GGNNCC 5 cut(s) 54, 75, 76, 258, 360
PspOMI GGGCCC 1 cut(s) 52
PspPI GGNCC 5 cut(s) 52, 53, 74, 128, 181
PspPPI RGGWCCY 1 cut(s) 74
PstI CTGCAG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 4 cut(s) 12, 187, 252, 329
RsaNI GTAC 4 cut(s) 11, 186, 251, 328
SapI GCTCTTC 1 cut(s) 153
SatI GCNGC 3 cut(s) 32, 344, 389
Sau3AI GATC 1 cut(s) 256
Sau96I GGNCC 5 cut(s) 52, 53, 74, 128, 181
ScaI AGTACT 1 cut(s) 329
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 2 cut(s) 56, 148
SetI ASST 5 cut(s) 12, 246, 345, 390, 401
SfcI CTRYAG 1 cut(s) 331
SinI GGWCC 2 cut(s) 74, 128
Sse9I AATT 2 cut(s) 349, 404
SsiI CCGC 1 cut(s) 31
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 118
TaaI ACNGT 2 cut(s) 161, 370
TaiI ACGT 2 cut(s) 12, 401
TaqI TCGA 2 cut(s) 175, 401
TasI AATT 2 cut(s) 349, 404
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 34
TscAI CASTG 2 cut(s) 54, 138
TseI GCWGC 2 cut(s) 343, 388
TspDTI ATGAA 2 cut(s) 140, 343
TspGWI ACGGA 2 cut(s) 258, 323
TspRI CASTG 2 cut(s) 54, 138
Van91I CCANNNNNTGG 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 74, 128
XapI RAATTY 2 cut(s) 349, 404
ZraI GACGTC 1 cut(s) 399
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.