Rmu_co8229999.1_g000001

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8229999.1
OG0000677
Physical Location & Seq
Forward (+)
1 .. 561
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8229999.1_g000001.1.cds

Sequence Viewer

Length: 561 bp
gcggatgtggtggatccggattcgggtatgagcgtgaaacgaaacgggtcggatgttggagaaatagttatgcgaggcgggtgcgtcatgttgggttacctcaaagacccggaaacgacgagcgaaaccctgaaacagaacgggtggttttacaccggcgacgtaggggttatgcacccggacgggtatctggagataaaggatcgatccaaggacgtgatcataagcgggggagagaacttgagcagcgtggaggtcgagtcagttctctacaccaacgccgccgtgaacgaggcggccgtggtggctaagcctgacgagttctggggggagacgccgtgcgcgtttgtgagcctgaaagctgacgtcaggccgaggccgaccgagaaagagataatagagttctgtagaggaaagatgccgaatttcatggtgccgaagacggtggtgttcaaggacgagctgccgaagacgtcgacggggaagattcagaagtctatgctgagagaaattgcgaaaaatattgggtctgcatcaacgacgaggtccagtcggttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

186

Amino Acids

20.33

Weight (kDa)

5.36

Isoelectric Point (pI)

34.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 369, 476
AccB1I GGYRCC 1 cut(s) 433
AccI GTMKAC 1 cut(s) 476
AccII CGCG 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 16
AciI CCGC 5 cut(s) 2, 78, 228, 282, 296
AclWI GGATC 4 cut(s) 8, 21, 201, 210
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 1 cut(s) 424
AcyI GRCGYC 3 cut(s) 335, 366, 473
AfiI CCNNNNNNNGG 1 cut(s) 23
AgsI TTSAA 1 cut(s) 454
AjiI CACGTC 1 cut(s) 217
AluBI AGCT 2 cut(s) 362, 463
AluI AGCT 2 cut(s) 362, 463
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 4 cut(s) 8, 21, 201, 210
Aor13HI TCCGGA 1 cut(s) 16
AoxI GGCC 3 cut(s) 297, 371, 377
ApeKI GCWGC 2 cut(s) 246, 463
ApoI RAATTY 1 cut(s) 424
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 1 cut(s) 546
AsuC2I CCSGG 2 cut(s) 110, 179
AvaII GGWCC 1 cut(s) 546
BamHI GGATCC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 433
BbsI GAAGAC 2 cut(s) 446, 476
BbvI GCAGC 2 cut(s) 258, 450
BceAI ACGGC 3 cut(s) 269, 284, 322
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BclI TGATCA 1 cut(s) 219
BcnI CCSGG 2 cut(s) 110, 179
BcoDI GTCTC 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 406
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 4 cut(s) 247, 282, 297, 464
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 4 cut(s) 248, 283, 298, 465
Bme1390I CCNGG 2 cut(s) 110, 179
Bme18I GGWCC 1 cut(s) 546
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 546
BmiI GGNNCC 2 cut(s) 15, 435
BmrFI CCNGG 2 cut(s) 110, 179
BmsI GCATC 2 cut(s) 408, 542
BpiI GAAGAC 2 cut(s) 446, 476
BpmI CTGGAG 1 cut(s) 212
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 262
BpuMI CCSGG 2 cut(s) 110, 179
Bsa29I ATCGAT 1 cut(s) 205
BsaHI GRCGYC 3 cut(s) 335, 366, 473
BsaJI CCNNGG 3 cut(s) 210, 300, 374
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 6 cut(s) 51, 81, 245, 275, 323, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 549
BseAI TCCGGA 1 cut(s) 16
BseCI ATCGAT 1 cut(s) 205
BseDI CCNNGG 3 cut(s) 210, 300, 374
BseGI GGATG 2 cut(s) 10, 58
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 1 cut(s) 549
BseX3I CGGCCG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 258, 450
Bsh1236I CGCG 1 cut(s) 344
Bsh1285I CGRYCG 2 cut(s) 300, 384
BshFI GGCC 3 cut(s) 299, 373, 379
BshNI GGYRCC 1 cut(s) 433
BshVI ATCGAT 1 cut(s) 205
BsiEI CGRYCG 2 cut(s) 300, 384
BsiSI CCGG 4 cut(s) 17, 110, 156, 179
BslI CCNNNNNNNGG 1 cut(s) 23
BsmAI GTCTC 1 cut(s) 326
BsmBI CGTCTC 1 cut(s) 326
BsnI GGCC 3 cut(s) 299, 373, 379
Bsp13I TCCGGA 1 cut(s) 16
Bsp143I GATC 4 cut(s) 13, 202, 206, 219
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 5 cut(s) 2, 78, 228, 282, 296
BspANI GGCC 3 cut(s) 299, 373, 379
BspCNI CTCAG 1 cut(s) 495
BspDI ATCGAT 1 cut(s) 205
BspEI TCCGGA 1 cut(s) 16
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 2 cut(s) 15, 435
BspPI GGATC 4 cut(s) 8, 21, 201, 210
BspT107I GGYRCC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 155
BsrI ACTGG 1 cut(s) 549
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 3 cut(s) 210, 300, 374
BssMI GATC 4 cut(s) 13, 202, 206, 219
BssNI GRCGYC 3 cut(s) 335, 366, 473
BssT1I CCWWGG 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 445
BstACI GRCGYC 3 cut(s) 335, 366, 473
BstDEI CTNAG 2 cut(s) 309, 503
BstDSI CCRYGG 1 cut(s) 300
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 2 cut(s) 10, 58
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 4 cut(s) 16, 205, 209, 222
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 4 cut(s) 13, 202, 206, 219
BstMCI CGRYCG 2 cut(s) 300, 384
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 2 cut(s) 108, 177
BstSFI CTRYAG 1 cut(s) 406
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 2 cut(s) 258, 450
BstV2I GAAGAC 2 cut(s) 446, 476
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BstZI CGGCCG 1 cut(s) 297
Bsu15I ATCGAT 1 cut(s) 205
BsuRI GGCC 3 cut(s) 299, 373, 379
BsuTUI ATCGAT 1 cut(s) 205
BtgI CCRYGG 1 cut(s) 300
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 2 cut(s) 10, 58
CfoI GCGC 1 cut(s) 344
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 205
CseI GACGC 2 cut(s) 73, 343
CviAII CATG 2 cut(s) 88, 430
CviJI RGCY 8 cut(s) 299, 308, 313, 354, 362, 373, 379, 463
CviKI_1 RGCY 8 cut(s) 299, 308, 313, 354, 362, 373, 379, 463
DdeI CTNAG 2 cut(s) 309, 503
DpnI GATC 4 cut(s) 15, 204, 208, 221
DpnII GATC 4 cut(s) 13, 202, 206, 219
EaeI YGGCCR 1 cut(s) 297
EagI CGGCCG 1 cut(s) 297
EclXI CGGCCG 1 cut(s) 297
Eco130I CCWWGG 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 546
Eco52I CGGCCG 1 cut(s) 297
Eco91I GGTNACC 1 cut(s) 95
EcoO65I GGTNACC 1 cut(s) 95
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 91, 433
FaiI YATR 7 cut(s) 29, 71, 89, 173, 224, 431, 500
FatI CATG 2 cut(s) 87, 429
FauI CCCGC 2 cut(s) 71, 221
FbaI TGATCA 1 cut(s) 219
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 4 cut(s) 247, 282, 297, 464
FokI GGATG 2 cut(s) 17, 65
Fsp4HI GCNGC 4 cut(s) 247, 282, 297, 464
GlaI GCGC 1 cut(s) 343
GluI GCNGC 4 cut(s) 247, 282, 297, 464
GsuI CTGGAG 1 cut(s) 212
HaeIII GGCC 3 cut(s) 299, 373, 379
HapII CCGG 4 cut(s) 17, 110, 156, 179
HgaI GACGC 2 cut(s) 73, 343
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 3 cut(s) 335, 366, 473
Hin1II CATG 2 cut(s) 91, 433
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HinfI GANTC 3 cut(s) 20, 260, 487
HpaII CCGG 4 cut(s) 17, 110, 156, 179
Hpy166II GTNNAC 2 cut(s) 289, 477
Hpy188I TCNGA 2 cut(s) 52, 492
Hpy188III TCNNGA 2 cut(s) 17, 191
Hpy8I GTNNAC 2 cut(s) 289, 477
Hpy99I CGWCG 5 cut(s) 121, 164, 478, 481, 544
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4IV ACGT 4 cut(s) 162, 216, 366, 473
HpyCH4V TGCA 2 cut(s) 175, 533
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 2 cut(s) 309, 503
HpySE526I ACGT 4 cut(s) 162, 216, 366, 473
Hsp92I GRCGYC 3 cut(s) 335, 366, 473
Hsp92II CATG 2 cut(s) 91, 433
HspAI GCGC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 16
Ksp22I TGATCA 1 cut(s) 219
Kzo9I GATC 4 cut(s) 13, 202, 206, 219
Lsp1109I GCAGC 2 cut(s) 258, 450
LweI GCATC 2 cut(s) 408, 542
MaeII ACGT 4 cut(s) 162, 216, 366, 473
MaeIII GTNAC 1 cut(s) 95
MalI GATC 4 cut(s) 15, 204, 208, 221
MboI GATC 4 cut(s) 13, 202, 206, 219
MboII GAAGA 3 cut(s) 451, 481, 496
MflI RGATCY 1 cut(s) 13
MluCI AATT 2 cut(s) 424, 510
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 30, 37
MnlI CCTC 7 cut(s) 68, 110, 247, 286, 369, 404, 537
MroI TCCGGA 1 cut(s) 16
MspI CCGG 4 cut(s) 17, 110, 156, 179
MspR9I CCNGG 2 cut(s) 110, 179
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 2 cut(s) 110, 179
NdeII GATC 4 cut(s) 13, 202, 206, 219
NlaIII CATG 2 cut(s) 91, 433
NlaIV GGNNCC 2 cut(s) 15, 435
NmeAIII GCCGAG 1 cut(s) 399
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 341
PfeI GAWTC 2 cut(s) 20, 487
PflFI GACNNNGTC 1 cut(s) 544
PkrI GCNGC 4 cut(s) 248, 283, 298, 465
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PspEI GGTNACC 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 15, 435
PspPI GGNCC 1 cut(s) 546
PsuI RGATCY 1 cut(s) 13
PsyI GACNNNGTC 1 cut(s) 544
SalI GTCGAC 1 cut(s) 475
SatI GCNGC 4 cut(s) 247, 282, 297, 464
Sau3AI GATC 4 cut(s) 13, 202, 206, 219
Sau96I GGNCC 1 cut(s) 546
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 2 cut(s) 110, 179
SetI ASST 9 cut(s) 102, 165, 219, 258, 364, 369, 465, 476, 548
SfaNI GCATC 2 cut(s) 408, 542
SfcI CTRYAG 1 cut(s) 406
SgrAI CRCCGGYG 1 cut(s) 155
SgrDI CGTCGACG 1 cut(s) 475
SinI GGWCC 1 cut(s) 546
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 2 cut(s) 424, 510
SsiI CCGC 5 cut(s) 2, 78, 228, 282, 296
SspI AATATT 1 cut(s) 523
StyD4I CCNGG 2 cut(s) 108, 177
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 445
TaiI ACGT 4 cut(s) 165, 219, 369, 476
TaqI TCGA 3 cut(s) 205, 258, 476
TaqII GACCGA 1 cut(s) 398
TasI AATT 2 cut(s) 424, 510
TauI GCSGC 2 cut(s) 284, 299
TfiI GAWTC 2 cut(s) 20, 487
TseI GCWGC 2 cut(s) 246, 463
TspDTI ATGAA 1 cut(s) 418
Tth111I GACNNNGTC 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 546
XapI RAATTY 1 cut(s) 424
XmiI GTMKAC 1 cut(s) 476
ZraI GACGTC 2 cut(s) 367, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.