Rw3G025020

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000677
Physical Location & Seq
Reverse (-)
32745894 .. 32747983
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025020.1

Sequence Viewer

Length: 1656 bp
ATGGAGGAGCTGAAGCCAACCCCCGCCAACTCATCCCCGCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGAACACACCACCTACACGTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCGTCCGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGACGCCCTCCATCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACGACGATGCACCCCATCCCAACTCCTCCGACTCATCCCCCTTCTGCGCCACGTACGAGGAAATGGTGGACAGCGGCGATCCGGACTTCCAGTGGGCCCTGCCGGAGAACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACTACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTATCCGTACGGGATCGCCGCCGTGGGAGGGACCAACGTCTGCGTCCGCAAATTCGACGCGCCTGTCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACCAATGTACCGAACAACGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCGCCAGCCGCGGTTCTGTCTCGGACTGAGTCGCTGGGATTCGTGGTGAGCCACGGGTACGGGTTAACGGAGACGGCGGGGCTGGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGGGTGAGGACGGTGGCCATGACCCGGGTGGACGTGGTGGATCCGGATACGGGGGTGCCGGTTAAACGCGACGGTTCGTCGCTTGGGGAGGTGGTTTTGAGAGGCGGGTGTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATCAAGGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACTCGGCGGTGAACGAGGCGGCCGTGGTGGCTAGGCCTGACGAGTTCTGGGGGGAGACTCCGTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTTATTGAGGAGCTGCCGAAGACGTCGACGGGGAAAATTCAGAAGTTCGTGCTGAGAGAGATGGCCAAGGCCTTGGGGTCCACAAGGCTGAGTCGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

551

Amino Acids

60.4

Weight (kDa)

6.57

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 403 1.4e-76 AMP-binding enzyme
AMP-binding_C PF13193 453 - 530 3e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 785
AatII GACGTC 1 cut(s) 1580
AccB1I GGYRCC 3 cut(s) 263, 1135, 1537
AccB7I CCANNNNNTGG 1 cut(s) 717
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 3 cut(s) 78, 351, 1580
AccII CGCG 7 cut(s) 153, 189, 782, 932, 1019, 1149, 1448
AccIII TCCGGA 2 cut(s) 517, 1123
AclWI GGATC 5 cut(s) 509, 743, 1115, 1128, 1305
AcoI YGGCCR 5 cut(s) 190, 1095, 1227, 1401, 1617
AcsI RAATTY 3 cut(s) 773, 1528, 1590
AcuI CTGAAG 2 cut(s) 32, 1088
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 5 cut(s) 180, 264, 381, 816, 1577
AfaI GTAC 7 cut(s) 233, 491, 666, 731, 867, 980, 1508
AfiI CCNNNNNNNGG 7 cut(s) 123, 260, 658, 717, 750, 1104, 1130
AflIII ACRYGT 2 cut(s) 120, 825
AhdI GACNNNNNGTC 4 cut(s) 138, 677, 815, 1156
AjiI CACGTC 5 cut(s) 196, 1114, 1267, 1321, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 560, 592
AluBI AGCT 8 cut(s) 10, 238, 340, 366, 723, 917, 1522, 1567
AluI AGCT 8 cut(s) 10, 238, 340, 366, 723, 917, 1522, 1567
Alw21I GWGCWC 2 cut(s) 240, 919
Alw26I GTCTC 5 cut(s) 205, 313, 945, 986, 1430
AlwI GGATC 5 cut(s) 509, 743, 1115, 1128, 1305
Ama87I CYCGRG 2 cut(s) 1028, 1104
Aor13HI TCCGGA 2 cut(s) 517, 1123
ApaI GGGCCC 1 cut(s) 535
ApeKI GCWGC 3 cut(s) 1350, 1522, 1567
ApoI RAATTY 3 cut(s) 773, 1528, 1590
AspLEI GCGC 6 cut(s) 266, 485, 784, 838, 1019, 1448
AspS9I GGNCC 9 cut(s) 531, 532, 553, 607, 660, 753, 844, 895, 1632
AsuC2I CCSGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
AsuHPI GGTGA 6 cut(s) 34, 628, 979, 1096, 1285, 1402
AvaI CYCGRG 2 cut(s) 1028, 1104
AvaII GGWCC 6 cut(s) 553, 607, 753, 844, 895, 1632
BaeGI GKGCMC 1 cut(s) 535
BaeI ACNNNNGTAYC 4 cut(s) 849, 882, 1119, 1152
BalI TGGCCA 2 cut(s) 1097, 1619
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 3 cut(s) 263, 1135, 1537
BanII GRGCYC 4 cut(s) 240, 535, 627, 919
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 1550, 1580
Bbv12I GWGCWC 2 cut(s) 240, 919
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 3 cut(s) 1362, 1509, 1554
BccI CCATC 5 cut(s) 173, 287, 396, 459, 1609
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciVI GTATCC 1 cut(s) 1120
BclI TGATCA 2 cut(s) 801, 1323
BcnI CCSGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
BcoDI GTCTC 5 cut(s) 205, 313, 945, 986, 1430
BfaI CTAG 2 cut(s) 59, 1413
BfmI CTRYAG 1 cut(s) 1510
BfoI RGCGCY 1 cut(s) 267
BfuI GTATCC 1 cut(s) 1120
BglI GCCNNNNNGGC 1 cut(s) 1409
BmcAI AGTACT 1 cut(s) 1508
Bme1390I CCNGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
Bme18I GGWCC 6 cut(s) 553, 607, 753, 844, 895, 1632
BmeRI GACNNNNNGTC 4 cut(s) 138, 677, 815, 1156
BmeT110I CYCGRG 2 cut(s) 1028, 1104
BmgBI CACGTC 5 cut(s) 196, 1114, 1267, 1321, 1471
BmgT120I GGNCC 9 cut(s) 531, 532, 553, 607, 660, 753, 844, 895, 1632
BmrFI CCNGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 3 cut(s) 134, 433, 1266
BmuI ACTGGG 1 cut(s) 667
BoxI GACNNNNGTC 1 cut(s) 758
BpiI GAAGAC 2 cut(s) 1550, 1580
BpmI CTGGAG 2 cut(s) 739, 1316
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 1366
BpuMI CCSGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
BsaAI YACGTR 2 cut(s) 123, 489
BsaHI GRCGYC 5 cut(s) 180, 264, 381, 816, 1577
BsaWI WCCGGW 2 cut(s) 517, 1123
BsaXI ACNNNNNCTCC 8 cut(s) 185, 215, 533, 563, 1349, 1379, 1427, 1457
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 260, 658, 717, 750, 1104, 1130
Bse118I RCCGGY 4 cut(s) 838, 1045, 1138, 1259
Bse1I ACTGG 3 cut(s) 526, 662, 1230
BseAI TCCGGA 2 cut(s) 517, 1123
BseGI GGATG 5 cut(s) 32, 219, 451, 470, 1656
BseLI CCNNNNNNNGG 7 cut(s) 123, 260, 658, 717, 750, 1104, 1130
BseMII CTCAG 4 cut(s) 376, 939, 1598, 1634
BseNI ACTGG 3 cut(s) 526, 662, 1230
BseRI GAGGAG 9 cut(s) 20, 160, 305, 308, 332, 410, 451, 641, 1577
BseSI GKGCMC 1 cut(s) 535
BseX3I CGGCCG 1 cut(s) 1401
BseXI GCAGC 3 cut(s) 1362, 1509, 1554
BseYI CCCAGC 1 cut(s) 955
Bsh1236I CGCG 7 cut(s) 153, 189, 782, 932, 1019, 1149, 1448
Bsh1285I CGRYCG 1 cut(s) 1404
BshNI GGYRCC 3 cut(s) 263, 1135, 1537
BsiEI CGRYCG 1 cut(s) 1404
BsiHKAI GWGCWC 2 cut(s) 240, 919
BsiHKCI CYCGRG 2 cut(s) 1028, 1104
BsiWI CGTACG 2 cut(s) 489, 729
BslFI GGGAC 3 cut(s) 236, 566, 766
BslI CCNNNNNNNGG 7 cut(s) 123, 260, 658, 717, 750, 1104, 1130
BsmAI GTCTC 5 cut(s) 205, 313, 945, 986, 1430
BsmBI CGTCTC 3 cut(s) 205, 313, 986
BsmFI GGGAC 3 cut(s) 236, 566, 766
BsoBI CYCGRG 2 cut(s) 1028, 1104
Bsp120I GGGCCC 1 cut(s) 531
Bsp1286I GDGCHC 4 cut(s) 240, 535, 627, 919
Bsp13I TCCGGA 2 cut(s) 517, 1123
Bsp143I GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
BspCNI CTCAG 4 cut(s) 375, 940, 1599, 1635
BspEI TCCGGA 2 cut(s) 517, 1123
BspFNI CGCG 7 cut(s) 153, 189, 782, 932, 1019, 1149, 1448
BspHI TCATGA 1 cut(s) 903
BspMAI CTGCAG 1 cut(s) 1514
BspPI GGATC 5 cut(s) 509, 743, 1115, 1128, 1305
BspQI GCTCTTC 1 cut(s) 632
BspT107I GGYRCC 3 cut(s) 263, 1135, 1537
BsrBI CCGCTC 1 cut(s) 40
BsrFI RCCGGY 4 cut(s) 838, 1045, 1138, 1259
BsrI ACTGG 3 cut(s) 526, 662, 1230
BssAI RCCGGY 4 cut(s) 838, 1045, 1138, 1259
BssMI GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
BssNI GRCGYC 5 cut(s) 180, 264, 381, 816, 1577
BssT1I CCWWGG 4 cut(s) 597, 1314, 1620, 1626
Bst4CI ACNGT 8 cut(s) 76, 140, 307, 640, 1093, 1154, 1247, 1549
Bst6I CTCTTC 3 cut(s) 350, 398, 632
BstACI GRCGYC 5 cut(s) 180, 264, 381, 816, 1577
BstBAI YACGTR 2 cut(s) 123, 489
BstC8I GCNNGC 2 cut(s) 927, 1047
BstDEI CTNAG 4 cut(s) 362, 948, 1607, 1643
BstDSI CCRYGG 4 cut(s) 744, 930, 973, 1404
BstF5I GGATG 5 cut(s) 32, 219, 451, 470, 1656
BstFNI CGCG 7 cut(s) 153, 189, 782, 932, 1019, 1149, 1448
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 6 cut(s) 266, 485, 784, 838, 1019, 1448
BstKTI GATC 8 cut(s) 517, 738, 804, 903, 1123, 1302, 1313, 1326
BstMAI GTCTC 5 cut(s) 205, 313, 945, 986, 1430
BstMBI GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
BstMCI CGRYCG 1 cut(s) 1404
BstMWI GCNNNNNNNGC 2 cut(s) 186, 1409
BstNSI RCATGY 2 cut(s) 829, 856
BstPAI GACNNNNGTC 1 cut(s) 758
BstSCI CCNGG 6 cut(s) 891, 1027, 1028, 1103, 1104, 1281
BstSFI CTRYAG 1 cut(s) 1510
BstSLI GKGCMC 1 cut(s) 535
BstUI CGCG 7 cut(s) 153, 189, 782, 932, 1019, 1149, 1448
BstV1I GCAGC 3 cut(s) 1362, 1509, 1554
BstV2I GAAGAC 2 cut(s) 1550, 1580
BstX2I RGATCY 2 cut(s) 1120, 1310
BstXI CCANNNNNNTGG 2 cut(s) 604, 1627
BstYI RGATCY 2 cut(s) 1120, 1310
BstZI CGGCCG 1 cut(s) 1401
BsuI GTATCC 1 cut(s) 1120
BtgI CCRYGG 4 cut(s) 744, 930, 973, 1404
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 5 cut(s) 196, 1114, 1267, 1321, 1471
BtsCI GGATG 5 cut(s) 32, 219, 451, 470, 1656
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 533, 610
Cac8I GCNNGC 2 cut(s) 927, 1047
CciI TCATGA 1 cut(s) 903
CfoI GCGC 6 cut(s) 266, 485, 784, 838, 1019, 1448
Cfr10I RCCGGY 4 cut(s) 838, 1045, 1138, 1259
Cfr13I GGNCC 9 cut(s) 531, 532, 553, 607, 660, 753, 844, 895, 1632
Cfr42I CCGCGG 1 cut(s) 933
Cfr9I CCCGGG 2 cut(s) 1028, 1104
CseI GACGC 9 cut(s) 142, 169, 195, 389, 571, 700, 754, 788, 805
Csp6I GTAC 7 cut(s) 232, 490, 665, 730, 866, 979, 1507
CviAII CATG 8 cut(s) 229, 256, 826, 853, 904, 1099, 1237, 1534
CviQI GTAC 7 cut(s) 232, 490, 665, 730, 866, 979, 1507
DdeI CTNAG 4 cut(s) 362, 948, 1607, 1643
DinI GGCGCC 1 cut(s) 265
DpnI GATC 8 cut(s) 516, 737, 803, 902, 1122, 1301, 1312, 1325
DpnII GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
DrdI GACNNNNNNGTC 1 cut(s) 785
DriI GACNNNNNGTC 4 cut(s) 138, 677, 815, 1156
DseDI GACNNNNNNGTC 1 cut(s) 785
EaeI YGGCCR 5 cut(s) 190, 1095, 1227, 1401, 1617
EagI CGGCCG 1 cut(s) 1401
Eam1104I CTCTTC 3 cut(s) 350, 398, 632
Eam1105I GACNNNNNGTC 4 cut(s) 138, 677, 815, 1156
EarI CTCTTC 3 cut(s) 350, 398, 632
EciI GGCGGA 3 cut(s) 311, 909, 1492
Ecl136II GAGCTC 2 cut(s) 238, 917
EclXI CGGCCG 1 cut(s) 1401
Eco130I CCWWGG 4 cut(s) 597, 1314, 1620, 1626
Eco147I AGGCCT 2 cut(s) 1417, 1625
Eco24I GRGCYC 4 cut(s) 240, 535, 627, 919
Eco47I GGWCC 6 cut(s) 553, 607, 753, 844, 895, 1632
Eco52I CGGCCG 1 cut(s) 1401
Eco53kI GAGCTC 2 cut(s) 238, 917
Eco57I CTGAAG 2 cut(s) 32, 1088
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 2 cut(s) 1028, 1104
EcoICRI GAGCTC 2 cut(s) 238, 917
EcoO109I RGGNCCY 2 cut(s) 532, 553
EcoT14I CCWWGG 4 cut(s) 597, 1314, 1620, 1626
EcoT38I GRGCYC 4 cut(s) 240, 535, 627, 919
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 4 cut(s) 597, 1314, 1620, 1626
Esp3I CGTCTC 3 cut(s) 205, 313, 986
FaeI CATG 8 cut(s) 232, 259, 829, 856, 907, 1102, 1240, 1537
FaiI YATR 9 cut(s) 230, 257, 827, 854, 905, 1100, 1238, 1328, 1535
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 236, 566, 766
FatI CATG 8 cut(s) 228, 255, 825, 852, 903, 1098, 1236, 1533
FauI CCCGC 8 cut(s) 31, 45, 232, 296, 308, 991, 1178, 1325
FbaI TGATCA 2 cut(s) 801, 1323
FblI GTMKAC 3 cut(s) 78, 351, 1580
FokI GGATG 4 cut(s) 19, 206, 438, 457
FriOI GRGCYC 4 cut(s) 240, 535, 627, 919
FspBI CTAG 2 cut(s) 59, 1413
GlaI GCGC 6 cut(s) 265, 484, 783, 837, 1018, 1447
GsaI CCCAGC 1 cut(s) 959
GsuI CTGGAG 2 cut(s) 739, 1316
HaeII RGCGCY 1 cut(s) 267
HgaI GACGC 9 cut(s) 142, 169, 195, 389, 571, 700, 754, 788, 805
HhaI GCGC 6 cut(s) 266, 485, 784, 838, 1019, 1448
Hin1I GRCGYC 5 cut(s) 180, 264, 381, 816, 1577
Hin1II CATG 8 cut(s) 232, 259, 829, 856, 907, 1102, 1240, 1537
Hin6I GCGC 6 cut(s) 264, 483, 782, 836, 1017, 1446
HinP1I GCGC 6 cut(s) 264, 483, 782, 836, 1017, 1446
HincII GTYRAC 4 cut(s) 352, 643, 987, 1581
HindII GTYRAC 4 cut(s) 352, 643, 987, 1581
HinfI GANTC 6 cut(s) 467, 950, 960, 1364, 1438, 1645
HpaI GTTAAC 1 cut(s) 987
HphI GGTGA 6 cut(s) 34, 628, 979, 1096, 1285, 1402
Hpy188I TCNGA 8 cut(s) 185, 466, 699, 806, 945, 1209, 1596, 1650
Hpy188III TCNNGA 6 cut(s) 518, 688, 898, 904, 1124, 1295
HpyAV CCTTC 3 cut(s) 102, 487, 1486
HpyCH4III ACNGT 8 cut(s) 76, 140, 307, 640, 1093, 1154, 1247, 1549
HpyCH4IV ACGT 9 cut(s) 122, 195, 488, 759, 1113, 1266, 1320, 1470, 1577
HpyCH4V TGCA 4 cut(s) 446, 1236, 1279, 1512
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 1409
HpyF3I CTNAG 4 cut(s) 362, 948, 1607, 1643
HpySE526I ACGT 9 cut(s) 122, 195, 488, 759, 1113, 1266, 1320, 1470, 1577
Hsp92I GRCGYC 5 cut(s) 180, 264, 381, 816, 1577
Hsp92II CATG 8 cut(s) 232, 259, 829, 856, 907, 1102, 1240, 1537
HspAI GCGC 6 cut(s) 264, 483, 782, 836, 1017, 1446
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 553
Kpn2I TCCGGA 2 cut(s) 517, 1123
KroI GCCGGC 1 cut(s) 1045
KroNI GCCGGC 1 cut(s) 1047
Ksp22I TGATCA 2 cut(s) 801, 1323
KspAI GTTAAC 1 cut(s) 987
KspI CCGCGG 1 cut(s) 933
Kzo9I GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 9 cut(s) 7, 126, 243, 345, 371, 720, 914, 922, 1564
Lsp1109I GCAGC 3 cut(s) 1362, 1509, 1554
LweI GCATC 3 cut(s) 134, 433, 1266
MaeI CTAG 2 cut(s) 59, 1413
MaeII ACGT 9 cut(s) 122, 195, 488, 759, 1113, 1266, 1320, 1470, 1577
MaeIII GTNAC 1 cut(s) 817
MalI GATC 8 cut(s) 516, 737, 803, 902, 1122, 1301, 1312, 1325
MbiI CCGCTC 1 cut(s) 40
MboI GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
MboII GAAGA 5 cut(s) 337, 385, 619, 1555, 1585
MflI RGATCY 2 cut(s) 1120, 1310
MhlI GDGCHC 4 cut(s) 240, 535, 627, 919
MlsI TGGCCA 2 cut(s) 1097, 1619
MluCI AATT 3 cut(s) 773, 1528, 1590
MluNI TGGCCA 2 cut(s) 1097, 1619
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 5 cut(s) 461, 959, 1373, 1432, 1654
MmeI TCCRAC 3 cut(s) 208, 489, 1628
Mox20I TGGCCA 2 cut(s) 1097, 1619
MroI TCCGGA 2 cut(s) 517, 1123
MroNI GCCGGC 1 cut(s) 1045
MscI TGGCCA 2 cut(s) 1097, 1619
MseI TTAA 2 cut(s) 986, 1143
MslI CAYNNNNRTG 1 cut(s) 824
Msp20I TGGCCA 2 cut(s) 1097, 1619
MspA1I CMGCKG 2 cut(s) 510, 932
MspR9I CCNGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
MvnI CGCG 7 cut(s) 153, 189, 782, 932, 1019, 1149, 1448
MwoI GCNNNNNNNGC 2 cut(s) 186, 1409
NaeI GCCGGC 1 cut(s) 1047
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
NdeII GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
NgoMIV GCCGGC 1 cut(s) 1045
NlaIII CATG 8 cut(s) 232, 259, 829, 856, 907, 1102, 1240, 1537
NmeAIII GCCGAG 3 cut(s) 30, 156, 1364
NmuCI GTSAC 1 cut(s) 817
NspI RCATGY 2 cut(s) 829, 856
PagI TCATGA 1 cut(s) 903
PceI AGGCCT 2 cut(s) 1417, 1625
PciI ACATGT 1 cut(s) 825
PciSI GCTCTTC 1 cut(s) 632
PcsI WCGNNNNNNNCGW 5 cut(s) 201, 438, 660, 1058, 1401
PdiI GCCGGC 1 cut(s) 1047
PfeI GAWTC 1 cut(s) 960
Pfl23II CGTACG 2 cut(s) 489, 729
PflFI GACNNNGTC 1 cut(s) 949
PflMI CCANNNNNTGG 1 cut(s) 717
PleI GAGTC 5 cut(s) 461, 958, 1372, 1432, 1653
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 5 cut(s) 461, 958, 1372, 1432, 1653
Ppu21I YACGTR 2 cut(s) 123, 489
PpuMI RGGWCCY 1 cut(s) 553
PscI ACATGT 1 cut(s) 825
PshAI GACNNNNGTC 1 cut(s) 758
Psp124BI GAGCTC 2 cut(s) 240, 919
Psp5II RGGWCCY 1 cut(s) 553
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 955
PspLI CGTACG 2 cut(s) 489, 729
PspOMI GGGCCC 1 cut(s) 531
PspPI GGNCC 9 cut(s) 531, 532, 553, 607, 660, 753, 844, 895, 1632
PspPPI RGGWCCY 1 cut(s) 553
PstI CTGCAG 1 cut(s) 1514
PsuI RGATCY 2 cut(s) 1120, 1310
PsyI GACNNNGTC 1 cut(s) 949
RsaI GTAC 7 cut(s) 233, 491, 666, 731, 867, 980, 1508
RsaNI GTAC 7 cut(s) 232, 490, 665, 730, 866, 979, 1507
RseI CAYNNNNRTG 1 cut(s) 824
SacI GAGCTC 2 cut(s) 240, 919
SacII CCGCGG 1 cut(s) 933
SalI GTCGAC 2 cut(s) 350, 1579
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 2 cut(s) 986, 1143
Sau3AI GATC 8 cut(s) 514, 735, 801, 900, 1120, 1299, 1310, 1323
Sau96I GGNCC 9 cut(s) 531, 532, 553, 607, 660, 753, 844, 895, 1632
ScaI AGTACT 1 cut(s) 1508
SchI GAGTC 5 cut(s) 461, 959, 1373, 1432, 1654
ScrFI CCNGG 6 cut(s) 893, 1029, 1030, 1105, 1106, 1283
SduI GDGCHC 4 cut(s) 240, 535, 627, 919
SfaNI GCATC 3 cut(s) 134, 433, 1266
SfcI CTRYAG 1 cut(s) 1510
SfoI GGCGCC 1 cut(s) 265
Sfr303I CCGCGG 1 cut(s) 933
SgrAI CRCCGGYG 2 cut(s) 838, 1259
SgrBI CCGCGG 1 cut(s) 933
SgrDI CGTCGACG 1 cut(s) 1579
SinI GGWCC 6 cut(s) 553, 607, 753, 844, 895, 1632
SmaI CCCGGG 2 cut(s) 1030, 1106
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 1 cut(s) 1345
SmoI CTYRAG 1 cut(s) 1345
Sse9I AATT 3 cut(s) 773, 1528, 1590
SseBI AGGCCT 2 cut(s) 1417, 1625
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 59, 1413
SstI GAGCTC 2 cut(s) 240, 919
StuI AGGCCT 2 cut(s) 1417, 1625
StyD4I CCNGG 6 cut(s) 891, 1027, 1028, 1103, 1104, 1281
StyI CCWWGG 4 cut(s) 597, 1314, 1620, 1626
TaaI ACNGT 8 cut(s) 76, 140, 307, 640, 1093, 1154, 1247, 1549
TaiI ACGT 9 cut(s) 125, 198, 491, 762, 1116, 1269, 1323, 1473, 1580
TaqI TCGA 8 cut(s) 142, 294, 351, 378, 654, 777, 1073, 1580
TaqII GACCGA 1 cut(s) 78
TasI AATT 3 cut(s) 773, 1528, 1590
TatI WGTACW 1 cut(s) 1506
TauI GCSGC 7 cut(s) 71, 192, 513, 743, 925, 932, 1403
TfiI GAWTC 1 cut(s) 960
Tru1I TTAA 2 cut(s) 986, 1143
Tru9I TTAA 2 cut(s) 986, 1143
TscAI CASTG 2 cut(s) 533, 617
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 3 cut(s) 1350, 1522, 1567
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 3 cut(s) 619, 1253, 1522
TspGWI ACGGA 6 cut(s) 193, 717, 1004, 1067, 1431, 1502
TspMI CCCGGG 2 cut(s) 1028, 1104
TspRI CASTG 2 cut(s) 533, 617
Tth111I GACNNNGTC 1 cut(s) 949
Van91I CCANNNNNTGG 1 cut(s) 717
VpaK11BI GGWCC 6 cut(s) 553, 607, 753, 844, 895, 1632
XapI RAATTY 3 cut(s) 773, 1528, 1590
XceI RCATGY 2 cut(s) 829, 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 1105
XmaI CCCGGG 2 cut(s) 1028, 1104
XmiI GTMKAC 3 cut(s) 78, 351, 1580
XspI CTAG 2 cut(s) 59, 1413
ZraI GACGTC 1 cut(s) 1578
ZrmI AGTACT 1 cut(s) 1508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.