AT1G77240

ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000677
Physical Location & Seq
Reverse (-)
29017958 .. 29019789
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77240.1

Sequence Viewer

Length: 1638 bp
ATGGAACTTTTACTCCCACACGCTTCAAACTCATGTCCTCTCACTGTTCTTGGCTTCTTAGAACGAGCCGCCTCTGTCTTCGGCGACTCTCCTTCACTCCTCCACACAACCACTGTTCATACTTGGTCTGAAACTCATTCTCGATGTCTCCGTATAGCTTCAACTCTCTCTTCCGCTTCCCTCGGAATCAACCGCGGCCAAGTTGTCTCTGTTATTGGTCCAAACGTCCCATCTGTCTACGAGCTTCAGTTCGCAGTTCCGATGTCAGGCGCAGTCCTCAACAACATCAACCCACGTTTAGACGCTCATGCACTCTCCGTCCTTCTACGTCACAGCGAATCCAAGCTCGTGTTTGTTGACCATCACTCAAGCTCTTTAGTCCTCGAAGCTGTCTCGTTTTTGCCCAAGGATGAGAGACCTCGTCTCGTCATACTCAACGACGGAAACGACATGCCATCATCTTCATCAGCTGATATGGATTTTCTTGACACGTACGAAGGGTTTATGGAGAGAGGAGACCTGAGGTTCAAGTGGGTACGTCCTAAAAGCGAGTGGACTCCAATGGTGCTTAACTACACCTCAGGAACTACGTCGTCCCCAAAGGGAGTGGTGCATAGCCACAGATCGGTTTTCATGAGCACTATTAACTCTTTGCTAGACTGGTCTTTGCCTAACCGTCCGGTATACCTGTGGACACTGCCGATGTTCCATGCCAATGGTTGGAGCTACACATGGGCCACAGCAGCTGTGGGAGCCAGAAACATCTGTGTCACAAGAGTTGATGTGCCGACTATTTTCAACTTGATCGACAAGTATCAAGTGACCCACATGTGCGCTGCGCCAATGGTTCTCAACATGTTGACTAATCACCCAGCCCAGAAACCGCTTCAGAGCCCAGTTAAGGTTATGACTGCTGGAGCTCCACCACCAGCTACAGTCATCTCCAAGGCTGAGGCGCTTGGTTTCGACGTGAGTCACGGTTACGGGATGACAGAAACAGGCGGTCTGGTTGTCTCGTGCGCATTGAAGCCTGAGTGGGATCGTCTAGAACCAGACGAGAGAGCAAAACAGAAATCAAGGCAAGGAATTAGAACTGCAGTATTCGCGGAAGTCGATGTTAGAGACCCAATATCCGGGAAGAGCGTGAAGCATGATGGAGCAACTGTGGGAGAGATCGTCTTCAGAGGCGGTTCGGTCATGCTAGGTTATTACAAAGACCCAGAAGGCACCGCAGCAAGTATGAGAGAAGACGGATGGTTCTACACCGGAGACATTGGAGTAATGCACCCAGACGGTTACTTGGAAGTCAAAGACAGATCAAAGGATGTGGTCATTTGCGGAGGAGAGAACATCAGCAGCACGGAACTCGAGGCAGTTCTGTACACAAACCCCGCAATTAAGGAAGCAGCTGTGGTGGCTAAGCCAGACAAGATGTGGGGAGAAACTCCATGTGCATTTGTCAGCCTGAAGTATCATGACGGGTCTGTGACTGAGAGAGAGATACGGGAGTTTTGTAAAACCAAGCTGCCAAAGTATATGGTTCCAAGGAATGTGGTTTTCCTGGAAGAGCTTCCAAAGACTTCTACTGGAAAGATTCAAAAGTTTCTTCTCAGACAAATGGCTAAGTCCTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

545

Amino Acids

60.01

Weight (kDa)

6.79

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 404 1.3e-78 AMP-binding enzyme
AMP-binding_C PF13193 455 - 531 5.3e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1021
AccB1I GGYRCC 1 cut(s) 1226
AccB7I CCANNNNNTGG 1 cut(s) 720
AccI GTMKAC 2 cut(s) 237, 684
AccII CGCG 2 cut(s) 195, 1106
AclWI GGATC 1 cut(s) 1047
AcoI YGGCCR 1 cut(s) 196
AcuI CTGAAG 4 cut(s) 230, 872, 1165, 1487
AfaI GTAC 3 cut(s) 494, 537, 1382
AfiI CCNNNNNNNGG 5 cut(s) 266, 625, 720, 901, 1133
AflIII ACRYGT 3 cut(s) 489, 828, 855
AgsI TTSAA 6 cut(s) 27, 162, 529, 799, 1027, 1598
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 1560
AloI GAACNNNNNNTCC 1 cut(s) 29
Alw21I GWGCWC 2 cut(s) 641, 922
Alw26I GTCTC 9 cut(s) 152, 211, 397, 409, 428, 510, 1018, 1116, 1263
AlwI GGATC 1 cut(s) 1047
AlwNI CAGNNNCTG 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 1367
AoxI GGCC 2 cut(s) 196, 735
ApeKI GCWGC 6 cut(s) 743, 836, 1232, 1356, 1406, 1525
Asp700I GAANNNNTTC 1 cut(s) 1569
AspLEI GCGC 5 cut(s) 272, 836, 841, 958, 1022
AspS9I GGNCC 2 cut(s) 218, 735
AsuC2I CCSGG 1 cut(s) 1135
AsuHPI GGTGA 1 cut(s) 860
AvaI CYCGRG 1 cut(s) 1367
AvaII GGWCC 1 cut(s) 218
AxyI CCTNAGG 2 cut(s) 521, 580
BaeI ACNNNNGTAYC 2 cut(s) 1493, 1526
BanI GGYRCC 1 cut(s) 1226
BanII GRGCYC 2 cut(s) 896, 922
BauI CACGAG 2 cut(s) 347, 1015
BbsI GAAGAC 3 cut(s) 70, 1171, 1254
Bbv12I GWGCWC 2 cut(s) 641, 922
BbvCI CCTCAGC 1 cut(s) 951
BbvI GCAGC 6 cut(s) 755, 823, 1244, 1368, 1418, 1512
BccI CCATC 5 cut(s) 238, 369, 463, 1148, 1248
BcgI CGANNNNNNTGC 2 cut(s) 1348, 1382
BciT130I CCWGG 1 cut(s) 1562
BcnI CCSGG 1 cut(s) 1135
BcoDI GTCTC 9 cut(s) 152, 211, 397, 409, 428, 510, 1018, 1116, 1263
BfaI CTAG 3 cut(s) 656, 1046, 1202
BfmI CTRYAG 2 cut(s) 933, 1095
BfoI RGCGCY 1 cut(s) 959
BisI GCNGC 8 cut(s) 69, 196, 744, 837, 1233, 1357, 1407, 1526
BlpI GCTNAGC 1 cut(s) 1419
BlsI GCNGC 8 cut(s) 70, 197, 745, 838, 1234, 1358, 1408, 1527
Bme1390I CCNGG 2 cut(s) 1135, 1562
Bme18I GGWCC 1 cut(s) 218
BmeT110I CYCGRG 1 cut(s) 1367
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 2 cut(s) 218, 735
BmiI GGNNCC 3 cut(s) 754, 1228, 1542
BmrFI CCNGG 2 cut(s) 1135, 1562
BmrI ACTGGG 1 cut(s) 890
BmuI ACTGGG 1 cut(s) 890
BoxI GACNNNNGTC 1 cut(s) 972
BpiI GAAGAC 3 cut(s) 70, 1171, 1254
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 1 cut(s) 951
Bpu1102I GCTNAGC 1 cut(s) 1419
BpuEI CTTGAG 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 1135
BsaAI YACGTR 1 cut(s) 492
BsaI GGTCTC 3 cut(s) 409, 510, 1116
BsaJI CCNNGG 5 cut(s) 181, 193, 405, 945, 1544
BsaWI WCCGGW 2 cut(s) 679, 1265
BsaXI ACNNNNNCTCC 5 cut(s) 27, 1149, 1161, 1179, 1191
Bsc4I CCNNNNNNNGG 5 cut(s) 266, 625, 720, 901, 1133
Bse1I ACTGG 3 cut(s) 665, 896, 1591
Bse21I CCTNAGG 2 cut(s) 521, 580
BseBI CCWGG 1 cut(s) 1562
BseDI CCNNGG 5 cut(s) 181, 193, 405, 945, 1544
BseGI GGATG 4 cut(s) 415, 993, 1259, 1330
BseLI CCNNNNNNNGG 5 cut(s) 266, 625, 720, 901, 1133
BseMII CTCAG 6 cut(s) 512, 594, 942, 1023, 1482, 1624
BseNI ACTGG 3 cut(s) 665, 896, 1591
BseRI GAGGAG 3 cut(s) 89, 528, 1356
BseXI GCAGC 6 cut(s) 755, 823, 1244, 1368, 1418, 1512
BseYI CCCAGC 1 cut(s) 871
Bsh1236I CGCG 2 cut(s) 195, 1106
BshFI GGCC 2 cut(s) 198, 737
BshNI GGYRCC 1 cut(s) 1226
BsiHKAI GWGCWC 2 cut(s) 641, 922
BsiHKCI CYCGRG 1 cut(s) 1367
BsiSI CCGG 3 cut(s) 680, 1134, 1266
BsiWI CGTACG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 212, 580
BslI CCNNNNNNNGG 5 cut(s) 266, 625, 720, 901, 1133
BsmAI GTCTC 9 cut(s) 152, 211, 397, 409, 428, 510, 1018, 1116, 1263
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 2 cut(s) 212, 580
BsnI GGCC 2 cut(s) 198, 737
Bso31I GGTCTC 3 cut(s) 409, 510, 1116
BsoBI CYCGRG 1 cut(s) 1367
Bsp1286I GDGCHC 3 cut(s) 641, 896, 922
Bsp1407I TGTACA 1 cut(s) 1380
Bsp143I GATC 5 cut(s) 623, 804, 1039, 1173, 1316
Bsp1720I GCTNAGC 1 cut(s) 1419
BspANI GGCC 2 cut(s) 198, 737
BspCNI CTCAG 6 cut(s) 513, 593, 943, 1024, 1483, 1623
BspFNI CGCG 2 cut(s) 195, 1106
BspHI TCATGA 2 cut(s) 633, 1474
BspLI GGNNCC 3 cut(s) 754, 1228, 1542
BspMAI CTGCAG 1 cut(s) 1099
BspPI GGATC 1 cut(s) 1047
BspQI GCTCTTC 2 cut(s) 1133, 1560
BspT107I GGYRCC 1 cut(s) 1226
BspTNI GGTCTC 3 cut(s) 409, 510, 1116
BsrGI TGTACA 1 cut(s) 1380
BsrI ACTGG 3 cut(s) 665, 896, 1591
BssECI CCNNGG 5 cut(s) 181, 193, 405, 945, 1544
BssMI GATC 5 cut(s) 623, 804, 1039, 1173, 1316
BssNAI GTATAC 1 cut(s) 685
BssSI CACGAG 2 cut(s) 347, 1015
BssT1I CCWWGG 3 cut(s) 405, 945, 1544
Bst1107I GTATAC 1 cut(s) 685
Bst2BI CACGAG 2 cut(s) 347, 1015
Bst2UI CCWGG 1 cut(s) 1562
Bst4CI ACNGT 7 cut(s) 46, 115, 677, 937, 980, 1165, 1295
Bst6I CTCTTC 3 cut(s) 175, 1133, 1560
BstAUI TGTACA 1 cut(s) 1380
BstBAI YACGTR 1 cut(s) 492
BstDEI CTNAG 9 cut(s) 58, 521, 580, 951, 1032, 1419, 1491, 1610, 1623
BstDSI CCRYGG 1 cut(s) 193
BstF5I GGATG 4 cut(s) 415, 993, 1259, 1330
BstFNI CGCG 2 cut(s) 195, 1106
BstH2I RGCGCY 1 cut(s) 959
BstHHI GCGC 5 cut(s) 272, 836, 841, 958, 1022
BstKTI GATC 5 cut(s) 626, 807, 1042, 1176, 1319
BstMAI GTCTC 9 cut(s) 152, 211, 397, 409, 428, 510, 1018, 1116, 1263
BstMBI GATC 5 cut(s) 623, 804, 1039, 1173, 1316
BstMWI GCNNNNNNNGC 4 cut(s) 743, 752, 1103, 1415
BstNI CCWGG 1 cut(s) 1562
BstNSI RCATGY 3 cut(s) 454, 832, 859
BstPAI GACNNNNGTC 1 cut(s) 972
BstSCI CCNGG 2 cut(s) 1133, 1560
BstSFI CTRYAG 2 cut(s) 933, 1095
BstUI CGCG 2 cut(s) 195, 1106
BstV1I GCAGC 6 cut(s) 755, 823, 1244, 1368, 1418, 1512
BstV2I GAAGAC 3 cut(s) 70, 1171, 1254
BstXI CCANNNNNNTGG 1 cut(s) 716
BstZ17I GTATAC 1 cut(s) 685
Bsu36I CCTNAGG 2 cut(s) 521, 580
BsuRI GGCC 2 cut(s) 198, 737
BtgI CCRYGG 1 cut(s) 193
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 4 cut(s) 415, 993, 1259, 1330
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 3 cut(s) 42, 111, 695
CaiI CAGNNNCTG 1 cut(s) 746
CciI TCATGA 2 cut(s) 633, 1474
CfoI GCGC 5 cut(s) 272, 836, 841, 958, 1022
Cfr13I GGNCC 2 cut(s) 218, 735
Cfr42I CCGCGG 1 cut(s) 196
CseI GACGC 1 cut(s) 311
Csp6I GTAC 3 cut(s) 493, 536, 1381
CspCI CAANNNNNGTGG 6 cut(s) 588, 623, 1308, 1343, 1533, 1568
CviQI GTAC 3 cut(s) 493, 536, 1381
DdeI CTNAG 9 cut(s) 58, 521, 580, 951, 1032, 1419, 1491, 1610, 1623
DpnI GATC 5 cut(s) 625, 806, 1041, 1175, 1318
DpnII GATC 5 cut(s) 623, 804, 1039, 1173, 1316
EaeI YGGCCR 1 cut(s) 196
Eam1104I CTCTTC 3 cut(s) 175, 1133, 1560
EarI CTCTTC 3 cut(s) 175, 1133, 1560
Ecl136II GAGCTC 1 cut(s) 920
Eco130I CCWWGG 3 cut(s) 405, 945, 1544
Eco24I GRGCYC 2 cut(s) 896, 922
Eco31I GGTCTC 3 cut(s) 409, 510, 1116
Eco47I GGWCC 1 cut(s) 218
Eco53kI GAGCTC 1 cut(s) 920
Eco57I CTGAAG 4 cut(s) 230, 872, 1165, 1487
Eco81I CCTNAGG 2 cut(s) 521, 580
Eco88I CYCGRG 1 cut(s) 1367
EcoICRI GAGCTC 1 cut(s) 920
EcoRII CCWGG 1 cut(s) 1560
EcoT14I CCWWGG 3 cut(s) 405, 945, 1544
EcoT38I GRGCYC 2 cut(s) 896, 922
ErhI CCWWGG 3 cut(s) 405, 945, 1544
Esp3I CGTCTC 1 cut(s) 428
FaqI GGGAC 2 cut(s) 212, 580
FauI CCCGC 1 cut(s) 1399
FblI GTMKAC 2 cut(s) 237, 684
Fnu4HI GCNGC 8 cut(s) 69, 196, 744, 837, 1233, 1357, 1407, 1526
FokI GGATG 4 cut(s) 422, 1000, 1266, 1337
FriOI GRGCYC 2 cut(s) 896, 922
Fsp4HI GCNGC 8 cut(s) 69, 196, 744, 837, 1233, 1357, 1407, 1526
FspAI RTGCGCAY 1 cut(s) 1021
FspBI CTAG 3 cut(s) 656, 1046, 1202
FspI TGCGCA 1 cut(s) 1021
GlaI GCGC 5 cut(s) 271, 835, 840, 957, 1021
GluI GCNGC 8 cut(s) 69, 196, 744, 837, 1233, 1357, 1407, 1526
GsaI CCCAGC 1 cut(s) 875
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 959
HaeIII GGCC 2 cut(s) 198, 737
HapII CCGG 3 cut(s) 680, 1134, 1266
HgaI GACGC 1 cut(s) 311
HhaI GCGC 5 cut(s) 272, 836, 841, 958, 1022
Hin6I GCGC 5 cut(s) 270, 834, 839, 956, 1020
HinP1I GCGC 5 cut(s) 270, 834, 839, 956, 1020
HincII GTYRAC 2 cut(s) 358, 861
HindII GTYRAC 2 cut(s) 358, 861
HinfI GANTC 6 cut(s) 86, 186, 338, 556, 973, 1594
HpaII CCGG 3 cut(s) 680, 1134, 1266
HphI GGTGA 1 cut(s) 860
Hpy166II GTNNAC 7 cut(s) 238, 358, 555, 685, 693, 861, 1383
Hpy188I TCNGA 6 cut(s) 130, 185, 261, 891, 1184, 1613
Hpy188III TCNNGA 6 cut(s) 141, 485, 582, 634, 1046, 1475
Hpy8I GTNNAC 7 cut(s) 238, 358, 555, 685, 693, 861, 1383
Hpy99I CGWCG 3 cut(s) 443, 595, 971
HpyAV CCTTC 4 cut(s) 102, 332, 491, 1217
HpyCH4III ACNGT 7 cut(s) 46, 115, 677, 937, 980, 1165, 1295
HpyCH4IV ACGT 7 cut(s) 225, 295, 328, 491, 538, 590, 969
HpyCH4V TGCA 5 cut(s) 311, 613, 1097, 1285, 1454
HpyF10VI GCNNNNNNNGC 4 cut(s) 743, 752, 1103, 1415
HpyF3I CTNAG 9 cut(s) 58, 521, 580, 951, 1032, 1419, 1491, 1610, 1623
HpySE526I ACGT 7 cut(s) 225, 295, 328, 491, 538, 590, 969
HspAI GCGC 5 cut(s) 270, 834, 839, 956, 1020
KspI CCGCGG 1 cut(s) 196
Kzo9I GATC 5 cut(s) 623, 804, 1039, 1173, 1316
LguI GCTCTTC 2 cut(s) 1133, 1560
LmnI GCTCC 5 cut(s) 723, 752, 917, 925, 1157
Lsp1109I GCAGC 6 cut(s) 755, 823, 1244, 1368, 1418, 1512
MaeI CTAG 3 cut(s) 656, 1046, 1202
MaeII ACGT 7 cut(s) 225, 295, 328, 491, 538, 590, 969
MaeIII GTNAC 7 cut(s) 329, 769, 820, 974, 980, 1295, 1486
MalI GATC 5 cut(s) 625, 806, 1041, 1175, 1318
MboI GATC 5 cut(s) 623, 804, 1039, 1173, 1316
MboII GAAGA 8 cut(s) 70, 162, 453, 1150, 1171, 1259, 1577, 1598
MhlI GDGCHC 3 cut(s) 641, 896, 922
MluCI AATT 2 cut(s) 1086, 1395
MlyI GAGTC 3 cut(s) 80, 550, 982
MmeI TCCRAC 1 cut(s) 701
MroXI GAANNNNTTC 1 cut(s) 1569
MseI TTAA 4 cut(s) 570, 645, 900, 1398
MslI CAYNNNNRTG 1 cut(s) 714
MspA1I CMGCKG 4 cut(s) 195, 470, 746, 1409
MspI CCGG 3 cut(s) 680, 1134, 1266
MspR9I CCNGG 2 cut(s) 1135, 1562
MteI GCGCNGCGC 1 cut(s) 837
MvaI CCWGG 1 cut(s) 1562
MvnI CGCG 2 cut(s) 195, 1106
MwoI GCNNNNNNNGC 4 cut(s) 743, 752, 1103, 1415
NciI CCSGG 1 cut(s) 1135
NdeII GATC 5 cut(s) 623, 804, 1039, 1173, 1316
NlaIV GGNNCC 3 cut(s) 754, 1228, 1542
NmuCI GTSAC 5 cut(s) 329, 769, 820, 974, 1486
NsbI TGCGCA 1 cut(s) 1021
NspI RCATGY 3 cut(s) 454, 832, 859
PaeR7I CTCGAG 1 cut(s) 1367
PagI TCATGA 2 cut(s) 633, 1474
PciI ACATGT 2 cut(s) 828, 855
PciSI GCTCTTC 2 cut(s) 1133, 1560
PcsI WCGNNNNNNNCGW 3 cut(s) 148, 444, 1110
PdmI GAANNNNTTC 1 cut(s) 1569
PfeI GAWTC 3 cut(s) 186, 338, 1594
Pfl23II CGTACG 1 cut(s) 492
PflFI GACNNNGTC 1 cut(s) 420
PflMI CCANNNNNTGG 1 cut(s) 720
PfoI TCCNGGA 2 cut(s) 1133, 1560
PkrI GCNGC 8 cut(s) 70, 197, 745, 838, 1234, 1358, 1408, 1527
PleI GAGTC 3 cut(s) 80, 550, 981
PpsI GAGTC 3 cut(s) 80, 550, 981
Ppu21I YACGTR 1 cut(s) 492
PscI ACATGT 2 cut(s) 828, 855
PshAI GACNNNNGTC 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 922
Psp6I CCWGG 1 cut(s) 1560
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 1 cut(s) 1560
PspLI CGTACG 1 cut(s) 492
PspN4I GGNNCC 3 cut(s) 754, 1228, 1542
PspPI GGNCC 2 cut(s) 218, 735
PspXI VCTCGAGB 1 cut(s) 1367
PstI CTGCAG 1 cut(s) 1099
PstNI CAGNNNCTG 1 cut(s) 746
PsyI GACNNNGTC 1 cut(s) 420
PvuII CAGCTG 3 cut(s) 470, 746, 1409
RsaI GTAC 3 cut(s) 494, 537, 1382
RsaNI GTAC 3 cut(s) 493, 536, 1381
RseI CAYNNNNRTG 1 cut(s) 714
SacI GAGCTC 1 cut(s) 922
SacII CCGCGG 1 cut(s) 196
SapI GCTCTTC 2 cut(s) 1133, 1560
SaqAI TTAA 4 cut(s) 570, 645, 900, 1398
SatI GCNGC 8 cut(s) 69, 196, 744, 837, 1233, 1357, 1407, 1526
Sau3AI GATC 5 cut(s) 623, 804, 1039, 1173, 1316
Sau96I GGNCC 2 cut(s) 218, 735
SchI GAGTC 3 cut(s) 80, 550, 982
ScrFI CCNGG 2 cut(s) 1135, 1562
SduI GDGCHC 3 cut(s) 641, 896, 922
SfcI CTRYAG 2 cut(s) 933, 1095
Sfr274I CTCGAG 1 cut(s) 1367
Sfr303I CCGCGG 1 cut(s) 196
SgrBI CCGCGG 1 cut(s) 196
SinI GGWCC 1 cut(s) 218
SlaI CTCGAG 1 cut(s) 1367
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 2 cut(s) 367, 1367
SmoI CTYRAG 2 cut(s) 367, 1367
Sse9I AATT 2 cut(s) 1086, 1395
SspMI CTAG 3 cut(s) 656, 1046, 1202
SstI GAGCTC 1 cut(s) 922
StyD4I CCNGG 2 cut(s) 1133, 1560
StyI CCWWGG 3 cut(s) 405, 945, 1544
TaaI ACNGT 7 cut(s) 46, 115, 677, 937, 980, 1165, 1295
TaiI ACGT 7 cut(s) 228, 298, 331, 494, 541, 593, 972
TaqI TCGA 6 cut(s) 142, 384, 807, 966, 1113, 1368
TaqII GACCGA 1 cut(s) 1183
TasI AATT 2 cut(s) 1086, 1395
TatI WGTACW 1 cut(s) 1380
TauI GCSGC 2 cut(s) 71, 198
TfiI GAWTC 3 cut(s) 186, 338, 1594
Tru1I TTAA 4 cut(s) 570, 645, 900, 1398
Tru9I TTAA 4 cut(s) 570, 645, 900, 1398
TscAI CASTG 3 cut(s) 49, 118, 702
TseFI GTSAC 5 cut(s) 329, 769, 820, 974, 1486
TseI GCWGC 6 cut(s) 743, 836, 1232, 1356, 1406, 1525
Tsp45I GTSAC 5 cut(s) 329, 769, 820, 974, 1486
TspDTI ATGAA 3 cut(s) 107, 453, 622
TspGWI ACGGA 5 cut(s) 140, 307, 456, 1266, 1376
TspRI CASTG 3 cut(s) 49, 118, 702
Tth111I GACNNNGTC 1 cut(s) 420
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 1 cut(s) 218
XbaI TCTAGA 1 cut(s) 1045
XceI RCATGY 3 cut(s) 454, 832, 859
XcmI CCANNNNNNNNNTGG 2 cut(s) 745, 1431
XhoI CTCGAG 1 cut(s) 1367
XmiI GTMKAC 2 cut(s) 237, 684
XmnI GAANNNNTTC 1 cut(s) 1569
XspI CTAG 3 cut(s) 656, 1046, 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.