RchiOBHm_Chr3g0487571

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000677
Physical Location & Seq
Reverse (-)
34766167 .. 34767783
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45200

Sequence Viewer

Length: 1464 bp
ATGGAGGAGCTGAAGCCAACCCCCGCCAACTCATCCCCGCTCAGCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGAACACACCACCTACACGTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCGTCCGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGACGCCCTCCATCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACGATGATGCACCTGCACCTGCACCCCATCCCAACTCCGACTCATCCCCGTTCTGCGCCACGTACGAGGAAATGGTGGAGAGCGGCGATCCGGACTTCCAGTGGACCCTACCGGAAGACGAGTGGGACCCGATGACACTGAATTACACTTCCGGCACGACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTGGGAGGGACCAACGTCTGCGTCCGCAAATTCGACGCGCCTGTCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACCAATGTACCGAACAGCGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCGCCCGCCGCGGTTCTGTCTCGGACCGAGTCGCTGGGATTCGTGGTGAGCCACGGGTACGGGTTGACCGAGACGGCGGGGCTAGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCCGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGGGTGAGGACGGTGGCCATGACCCGGGTGGACGTGGTGGATCCGGATACGGGGTTGCCGGTTAAACGCGACGGTTCGTCGCTTGGGGAGGTGGTTTTCAGAGGCGGGTGTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATCAAGGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTACTCTACACCAACTCCGCGGTGAACGAGGCGGCCGTGGTGGCGAGGCCTGACGAGTTCTGGGGGAGACACCGTGCGCGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

487

Amino Acids

53.17

Weight (kDa)

6.24

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 406 4.4e-77 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 457, 463
AasI GACNNNNNNGTC 1 cut(s) 794
Acc36I ACCTGC 2 cut(s) 457, 463
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 726
AccBSI CCGCTC 2 cut(s) 40, 519
AccI GTMKAC 2 cut(s) 78, 351
AccII CGCG 9 cut(s) 153, 189, 791, 941, 1028, 1058, 1158, 1397, 1456
AccIII TCCGGA 2 cut(s) 526, 1132
AclWI GGATC 5 cut(s) 518, 752, 1124, 1137, 1314
AcoI YGGCCR 4 cut(s) 190, 1104, 1236, 1410
AcsI RAATTY 1 cut(s) 782
AcuI CTGAAG 2 cut(s) 32, 1097
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 4 cut(s) 180, 264, 381, 825
AfaI GTAC 7 cut(s) 233, 500, 675, 740, 876, 989, 1380
AfiI CCNNNNNNNGG 7 cut(s) 123, 260, 667, 726, 759, 1113, 1139
AflIII ACRYGT 2 cut(s) 120, 834
AhdI GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
AjiI CACGTC 4 cut(s) 196, 1123, 1276, 1330
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 6 cut(s) 10, 238, 340, 366, 732, 926
AluI AGCT 6 cut(s) 10, 238, 340, 366, 732, 926
Alw21I GWGCWC 2 cut(s) 240, 928
Alw26I GTCTC 5 cut(s) 205, 313, 954, 995, 1438
AlwI GGATC 5 cut(s) 518, 752, 1124, 1137, 1314
Ama87I CYCGRG 2 cut(s) 1037, 1113
Aor13HI TCCGGA 2 cut(s) 526, 1132
AoxI GGCC 6 cut(s) 190, 669, 1104, 1236, 1410, 1424
ApeKI GCWGC 1 cut(s) 1359
ApoI RAATTY 1 cut(s) 782
AspLEI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1456
AspS9I GGNCC 8 cut(s) 540, 562, 616, 669, 762, 853, 904, 954
AsuC2I CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
AsuHPI GGTGA 5 cut(s) 637, 988, 1105, 1294, 1411
AvaI CYCGRG 2 cut(s) 1037, 1113
AvaII GGWCC 7 cut(s) 540, 562, 616, 762, 853, 904, 954
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BalI TGGCCA 1 cut(s) 1106
BamHI GGATCC 1 cut(s) 1129
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 3 cut(s) 240, 636, 928
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 2 cut(s) 240, 928
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 1371
BccI CCATC 4 cut(s) 173, 287, 396, 471
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciVI GTATCC 1 cut(s) 1129
BclI TGATCA 2 cut(s) 810, 1332
BcnI CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
BcoDI GTCTC 5 cut(s) 205, 313, 954, 995, 1438
BfaI CTAG 2 cut(s) 59, 1013
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 2 cut(s) 457, 463
BfuI GTATCC 1 cut(s) 1129
BglI GCCNNNNNGGC 2 cut(s) 51, 1418
BisI GCNGC 8 cut(s) 69, 190, 520, 750, 932, 939, 1360, 1410
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 8 cut(s) 70, 191, 521, 751, 933, 940, 1361, 1411
Bme1390I CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
Bme18I GGWCC 7 cut(s) 540, 562, 616, 762, 853, 904, 954
BmeRI GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
BmeT110I CYCGRG 2 cut(s) 1037, 1113
BmgBI CACGTC 4 cut(s) 196, 1123, 1276, 1330
BmgT120I GGNCC 8 cut(s) 540, 562, 616, 669, 762, 853, 904, 954
BmiI GGNNCC 8 cut(s) 128, 265, 542, 563, 564, 763, 899, 1131
BmrFI CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
BmrI ACTGGG 1 cut(s) 676
BmsI GCATC 3 cut(s) 134, 433, 1275
BmuI ACTGGG 1 cut(s) 676
BoxI GACNNNNGTC 1 cut(s) 767
BpiI GAAGAC 1 cut(s) 558
BpmI CTGGAG 2 cut(s) 748, 1325
Bpu10I CCTNAGC 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 1375
BpuMI CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
BsaAI YACGTR 2 cut(s) 123, 498
BsaHI GRCGYC 4 cut(s) 180, 264, 381, 825
BsaWI WCCGGW 3 cut(s) 526, 547, 1132
BsaXI ACNNNNNCTCC 7 cut(s) 185, 215, 1358, 1376, 1388, 1406, 1435
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 260, 667, 726, 759, 1113, 1139
Bse118I RCCGGY 3 cut(s) 847, 1147, 1268
Bse1I ACTGG 3 cut(s) 535, 671, 1239
BseAI TCCGGA 2 cut(s) 526, 1132
BseGI GGATG 4 cut(s) 32, 219, 463, 479
BseLI CCNNNNNNNGG 7 cut(s) 123, 260, 667, 726, 759, 1113, 1139
BseMII CTCAG 2 cut(s) 55, 376
BseNI ACTGG 3 cut(s) 535, 671, 1239
BseRI GAGGAG 7 cut(s) 20, 160, 305, 308, 332, 410, 650
BseX3I CGGCCG 1 cut(s) 1410
BseXI GCAGC 1 cut(s) 1371
BseYI CCCAGC 1 cut(s) 964
BsgI GTGCAG 2 cut(s) 435, 441
Bsh1236I CGCG 9 cut(s) 153, 189, 791, 941, 1028, 1058, 1158, 1397, 1456
Bsh1285I CGRYCG 1 cut(s) 1413
BshFI GGCC 6 cut(s) 192, 671, 1106, 1238, 1412, 1426
BshNI GGYRCC 1 cut(s) 263
BsiEI CGRYCG 1 cut(s) 1413
BsiHKAI GWGCWC 2 cut(s) 240, 928
BsiHKCI CYCGRG 2 cut(s) 1037, 1113
BsiWI CGTACG 2 cut(s) 498, 738
BslFI GGGAC 3 cut(s) 236, 575, 775
BslI CCNNNNNNNGG 7 cut(s) 123, 260, 667, 726, 759, 1113, 1139
BsmAI GTCTC 5 cut(s) 205, 313, 954, 995, 1438
BsmBI CGTCTC 3 cut(s) 205, 313, 995
BsmFI GGGAC 3 cut(s) 236, 575, 775
BsnI GGCC 6 cut(s) 192, 671, 1106, 1238, 1412, 1426
BsoBI CYCGRG 2 cut(s) 1037, 1113
Bsp1286I GDGCHC 3 cut(s) 240, 636, 928
Bsp13I TCCGGA 2 cut(s) 526, 1132
Bsp143I GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
Bsp1720I GCTNAGC 1 cut(s) 41
BspANI GGCC 6 cut(s) 192, 671, 1106, 1238, 1412, 1426
BspCNI CTCAG 2 cut(s) 54, 375
BspEI TCCGGA 2 cut(s) 526, 1132
BspFNI CGCG 9 cut(s) 153, 189, 791, 941, 1028, 1058, 1158, 1397, 1456
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 8 cut(s) 128, 265, 542, 563, 564, 763, 899, 1131
BspMI ACCTGC 2 cut(s) 457, 463
BspPI GGATC 5 cut(s) 518, 752, 1124, 1137, 1314
BspQI GCTCTTC 1 cut(s) 641
BspT107I GGYRCC 1 cut(s) 263
BsrBI CCGCTC 2 cut(s) 40, 519
BsrFI RCCGGY 3 cut(s) 847, 1147, 1268
BsrI ACTGG 3 cut(s) 535, 671, 1239
BssAI RCCGGY 3 cut(s) 847, 1147, 1268
BssMI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
BssNI GRCGYC 4 cut(s) 180, 264, 381, 825
BssT1I CCWWGG 2 cut(s) 606, 1323
Bst4CI ACNGT 8 cut(s) 76, 140, 307, 649, 1102, 1163, 1256, 1451
Bst6I CTCTTC 3 cut(s) 350, 398, 641
BstACI GRCGYC 4 cut(s) 180, 264, 381, 825
BstBAI YACGTR 2 cut(s) 123, 498
BstC8I GCNNGC 3 cut(s) 887, 936, 1056
BstDEI CTNAG 2 cut(s) 41, 362
BstDSI CCRYGG 5 cut(s) 753, 939, 982, 1395, 1413
BstF5I GGATG 4 cut(s) 32, 219, 463, 479
BstFNI CGCG 9 cut(s) 153, 189, 791, 941, 1028, 1058, 1158, 1397, 1456
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1456
BstKTI GATC 8 cut(s) 526, 747, 813, 912, 1132, 1311, 1322, 1335
BstMAI GTCTC 5 cut(s) 205, 313, 954, 995, 1438
BstMBI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
BstMCI CGRYCG 1 cut(s) 1413
BstMWI GCNNNNNNNGC 3 cut(s) 51, 186, 1418
BstNSI RCATGY 2 cut(s) 838, 865
BstPAI GACNNNNGTC 1 cut(s) 767
BstSCI CCNGG 6 cut(s) 900, 1036, 1037, 1112, 1113, 1290
BstUI CGCG 9 cut(s) 153, 189, 791, 941, 1028, 1058, 1158, 1397, 1456
BstV1I GCAGC 1 cut(s) 1371
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 2 cut(s) 1129, 1319
BstXI CCANNNNNNTGG 1 cut(s) 613
BstYI RGATCY 2 cut(s) 1129, 1319
BstZI CGGCCG 1 cut(s) 1410
BsuI GTATCC 1 cut(s) 1129
BsuRI GGCC 6 cut(s) 192, 671, 1106, 1238, 1412, 1426
BtgI CCRYGG 5 cut(s) 753, 939, 982, 1395, 1413
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 4 cut(s) 196, 1123, 1276, 1330
BtsCI GGATG 4 cut(s) 32, 219, 463, 479
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 3 cut(s) 542, 572, 619
BveI ACCTGC 2 cut(s) 457, 463
Cac8I GCNNGC 3 cut(s) 887, 936, 1056
CciI TCATGA 1 cut(s) 912
CfoI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1456
Cfr10I RCCGGY 3 cut(s) 847, 1147, 1268
Cfr13I GGNCC 8 cut(s) 540, 562, 616, 669, 762, 853, 904, 954
Cfr42I CCGCGG 2 cut(s) 942, 1398
Cfr9I CCCGGG 2 cut(s) 1037, 1113
CpoI CGGWCCG 1 cut(s) 954
CseI GACGC 8 cut(s) 142, 169, 195, 389, 709, 763, 797, 814
Csp6I GTAC 7 cut(s) 232, 499, 674, 739, 875, 988, 1379
CspI CGGWCCG 1 cut(s) 954
CviAII CATG 7 cut(s) 229, 256, 835, 862, 913, 1108, 1246
CviQI GTAC 7 cut(s) 232, 499, 674, 739, 875, 988, 1379
DdeI CTNAG 2 cut(s) 41, 362
DinI GGCGCC 1 cut(s) 265
DpnI GATC 8 cut(s) 525, 746, 812, 911, 1131, 1310, 1321, 1334
DpnII GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
DrdI GACNNNNNNGTC 1 cut(s) 794
DriI GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
DseDI GACNNNNNNGTC 1 cut(s) 794
EaeI YGGCCR 4 cut(s) 190, 1104, 1236, 1410
EagI CGGCCG 1 cut(s) 1410
Eam1104I CTCTTC 3 cut(s) 350, 398, 641
Eam1105I GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
EarI CTCTTC 3 cut(s) 350, 398, 641
EciI GGCGGA 2 cut(s) 311, 918
Ecl136II GAGCTC 2 cut(s) 238, 926
EclXI CGGCCG 1 cut(s) 1410
Eco130I CCWWGG 2 cut(s) 606, 1323
Eco147I AGGCCT 1 cut(s) 1426
Eco24I GRGCYC 3 cut(s) 240, 636, 928
Eco47I GGWCC 7 cut(s) 540, 562, 616, 762, 853, 904, 954
Eco52I CGGCCG 1 cut(s) 1410
Eco53kI GAGCTC 2 cut(s) 238, 926
Eco57I CTGAAG 2 cut(s) 32, 1097
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 2 cut(s) 1037, 1113
EcoICRI GAGCTC 2 cut(s) 238, 926
EcoO109I RGGNCCY 1 cut(s) 562
EcoT14I CCWWGG 2 cut(s) 606, 1323
EcoT38I GRGCYC 3 cut(s) 240, 636, 928
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 606, 1323
Esp3I CGTCTC 3 cut(s) 205, 313, 995
FaeI CATG 7 cut(s) 232, 259, 838, 865, 916, 1111, 1249
FaiI YATR 8 cut(s) 230, 257, 836, 863, 914, 1109, 1247, 1337
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 236, 575, 775
FatI CATG 7 cut(s) 228, 255, 834, 861, 912, 1107, 1245
FbaI TGATCA 2 cut(s) 810, 1332
FblI GTMKAC 2 cut(s) 78, 351
Fnu4HI GCNGC 8 cut(s) 69, 190, 520, 750, 932, 939, 1360, 1410
FokI GGATG 4 cut(s) 19, 206, 450, 466
FriOI GRGCYC 3 cut(s) 240, 636, 928
Fsp4HI GCNGC 8 cut(s) 69, 190, 520, 750, 932, 939, 1360, 1410
FspBI CTAG 2 cut(s) 59, 1013
GlaI GCGC 6 cut(s) 265, 493, 792, 846, 1027, 1455
GluI GCNGC 8 cut(s) 69, 190, 520, 750, 932, 939, 1360, 1410
GsaI CCCAGC 1 cut(s) 968
GsuI CTGGAG 2 cut(s) 748, 1325
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 6 cut(s) 192, 671, 1106, 1238, 1412, 1426
HgaI GACGC 8 cut(s) 142, 169, 195, 389, 709, 763, 797, 814
HhaI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1456
Hin1I GRCGYC 4 cut(s) 180, 264, 381, 825
Hin1II CATG 7 cut(s) 232, 259, 838, 865, 916, 1111, 1249
Hin6I GCGC 6 cut(s) 264, 492, 791, 845, 1026, 1454
HinP1I GCGC 6 cut(s) 264, 492, 791, 845, 1026, 1454
HincII GTYRAC 3 cut(s) 352, 652, 996
HindII GTYRAC 3 cut(s) 352, 652, 996
HinfI GANTC 4 cut(s) 476, 959, 969, 1373
HphI GGTGA 5 cut(s) 637, 988, 1105, 1294, 1411
Hpy166II GTNNAC 8 cut(s) 79, 352, 540, 619, 652, 996, 1120, 1402
Hpy188I TCNGA 7 cut(s) 185, 475, 708, 815, 954, 1190, 1218
Hpy188III TCNNGA 6 cut(s) 527, 697, 907, 913, 1133, 1304
Hpy8I GTNNAC 8 cut(s) 79, 352, 540, 619, 652, 996, 1120, 1402
HpyAV CCTTC 1 cut(s) 102
HpyCH4III ACNGT 8 cut(s) 76, 140, 307, 649, 1102, 1163, 1256, 1451
HpyCH4IV ACGT 7 cut(s) 122, 195, 497, 768, 1122, 1275, 1329
HpyCH4V TGCA 5 cut(s) 446, 452, 458, 1245, 1288
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 186, 1418
HpyF3I CTNAG 2 cut(s) 41, 362
HpySE526I ACGT 7 cut(s) 122, 195, 497, 768, 1122, 1275, 1329
Hsp92I GRCGYC 4 cut(s) 180, 264, 381, 825
Hsp92II CATG 7 cut(s) 232, 259, 838, 865, 916, 1111, 1249
HspAI GCGC 6 cut(s) 264, 492, 791, 845, 1026, 1454
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 562
Kpn2I TCCGGA 2 cut(s) 526, 1132
Ksp22I TGATCA 2 cut(s) 810, 1332
KspI CCGCGG 2 cut(s) 942, 1398
Kzo9I GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 8 cut(s) 7, 126, 243, 345, 371, 729, 923, 931
Lsp1109I GCAGC 1 cut(s) 1371
LweI GCATC 3 cut(s) 134, 433, 1275
MaeI CTAG 2 cut(s) 59, 1013
MaeII ACGT 7 cut(s) 122, 195, 497, 768, 1122, 1275, 1329
MaeIII GTNAC 1 cut(s) 826
MalI GATC 8 cut(s) 525, 746, 812, 911, 1131, 1310, 1321, 1334
MbiI CCGCTC 2 cut(s) 40, 519
MboI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
MboII GAAGA 4 cut(s) 337, 385, 563, 628
MflI RGATCY 2 cut(s) 1129, 1319
MhlI GDGCHC 3 cut(s) 240, 636, 928
MlsI TGGCCA 1 cut(s) 1106
MluCI AATT 2 cut(s) 577, 782
MluNI TGGCCA 1 cut(s) 1106
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 3 cut(s) 470, 968, 1382
MmeI TCCRAC 2 cut(s) 208, 498
Mox20I TGGCCA 1 cut(s) 1106
MroI TCCGGA 2 cut(s) 526, 1132
MscI TGGCCA 1 cut(s) 1106
MseI TTAA 1 cut(s) 1152
MslI CAYNNNNRTG 1 cut(s) 833
Msp20I TGGCCA 1 cut(s) 1106
MspA1I CMGCKG 2 cut(s) 941, 1397
MspR9I CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
MvnI CGCG 9 cut(s) 153, 189, 791, 941, 1028, 1058, 1158, 1397, 1456
MwoI GCNNNNNNNGC 3 cut(s) 51, 186, 1418
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
NdeII GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
NlaIII CATG 7 cut(s) 232, 259, 838, 865, 916, 1111, 1249
NlaIV GGNNCC 8 cut(s) 128, 265, 542, 563, 564, 763, 899, 1131
NmeAIII GCCGAG 2 cut(s) 30, 156
NmuCI GTSAC 1 cut(s) 826
NspI RCATGY 2 cut(s) 838, 865
PagI TCATGA 1 cut(s) 912
PaqCI CACCTGC 2 cut(s) 457, 463
PceI AGGCCT 1 cut(s) 1426
PciI ACATGT 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 5 cut(s) 201, 669, 996, 1067, 1410
PfeI GAWTC 1 cut(s) 969
Pfl23II CGTACG 2 cut(s) 498, 738
PflFI GACNNNGTC 1 cut(s) 958
PflMI CCANNNNNTGG 1 cut(s) 726
PkrI GCNGC 8 cut(s) 70, 191, 521, 751, 933, 940, 1361, 1411
PleI GAGTC 3 cut(s) 470, 967, 1381
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 3 cut(s) 470, 967, 1381
Ppu21I YACGTR 2 cut(s) 123, 498
PpuMI RGGWCCY 1 cut(s) 562
PscI ACATGT 1 cut(s) 834
PshAI GACNNNNGTC 1 cut(s) 767
Psp124BI GAGCTC 2 cut(s) 240, 928
Psp5II RGGWCCY 1 cut(s) 562
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 964
PspLI CGTACG 2 cut(s) 498, 738
PspN4I GGNNCC 8 cut(s) 128, 265, 542, 563, 564, 763, 899, 1131
PspPI GGNCC 8 cut(s) 540, 562, 616, 669, 762, 853, 904, 954
PspPPI RGGWCCY 1 cut(s) 562
PsuI RGATCY 2 cut(s) 1129, 1319
PsyI GACNNNGTC 1 cut(s) 958
RsaI GTAC 7 cut(s) 233, 500, 675, 740, 876, 989, 1380
RsaNI GTAC 7 cut(s) 232, 499, 674, 739, 875, 988, 1379
RseI CAYNNNNRTG 1 cut(s) 833
Rsr2I CGGWCCG 1 cut(s) 954
RsrII CGGWCCG 1 cut(s) 954
SacI GAGCTC 2 cut(s) 240, 928
SacII CCGCGG 2 cut(s) 942, 1398
SalI GTCGAC 1 cut(s) 350
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 1 cut(s) 1152
SatI GCNGC 8 cut(s) 69, 190, 520, 750, 932, 939, 1360, 1410
Sau3AI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
Sau96I GGNCC 8 cut(s) 540, 562, 616, 669, 762, 853, 904, 954
SchI GAGTC 3 cut(s) 470, 968, 1382
ScrFI CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
SduI GDGCHC 3 cut(s) 240, 636, 928
SfaNI GCATC 3 cut(s) 134, 433, 1275
SfoI GGCGCC 1 cut(s) 265
Sfr303I CCGCGG 2 cut(s) 942, 1398
SgrAI CRCCGGYG 2 cut(s) 847, 1268
SgrBI CCGCGG 2 cut(s) 942, 1398
SinI GGWCC 7 cut(s) 540, 562, 616, 762, 853, 904, 954
SmaI CCCGGG 2 cut(s) 1039, 1115
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 1354
SmoI CTYRAG 1 cut(s) 1354
Sse9I AATT 2 cut(s) 577, 782
SseBI AGGCCT 1 cut(s) 1426
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 59, 1013
SstI GAGCTC 2 cut(s) 240, 928
StuI AGGCCT 1 cut(s) 1426
StyD4I CCNGG 6 cut(s) 900, 1036, 1037, 1112, 1113, 1290
StyI CCWWGG 2 cut(s) 606, 1323
TaaI ACNGT 8 cut(s) 76, 140, 307, 649, 1102, 1163, 1256, 1451
TaiI ACGT 7 cut(s) 125, 198, 500, 771, 1125, 1278, 1332
TaqI TCGA 7 cut(s) 142, 294, 351, 378, 663, 786, 1082
TaqII GACCGA 3 cut(s) 78, 971, 1013
TasI AATT 2 cut(s) 577, 782
TauI GCSGC 7 cut(s) 71, 192, 522, 752, 934, 941, 1412
TfiI GAWTC 1 cut(s) 969
Tru1I TTAA 1 cut(s) 1152
Tru9I TTAA 1 cut(s) 1152
TscAI CASTG 3 cut(s) 542, 579, 626
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 1 cut(s) 1359
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 2 cut(s) 628, 1262
TspGWI ACGGA 2 cut(s) 193, 1076
TspMI CCCGGG 2 cut(s) 1037, 1113
TspRI CASTG 3 cut(s) 542, 579, 626
Tth111I GACNNNGTC 1 cut(s) 958
Van91I CCANNNNNTGG 1 cut(s) 726
VpaK11BI GGWCC 7 cut(s) 540, 562, 616, 762, 853, 904, 954
XapI RAATTY 1 cut(s) 782
XceI RCATGY 2 cut(s) 838, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XmaI CCCGGG 2 cut(s) 1037, 1113
XmiI GTMKAC 2 cut(s) 78, 351
XspI CTAG 2 cut(s) 59, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.