RchiOBHm_Chr3g0487741

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000677
Physical Location & Seq
Reverse (-)
35028814 .. 35030816
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45216

Sequence Viewer

Length: 1605 bp
ATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCATCGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGATGCCCTCCATCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACCATGATGCACCCCATCCCAACTCCTCCGACTCATCCCCCTTCTGCGCCACGTACGAGGAAATGGTGGAGAGCGGCGATCCGGACTTCCAGTGGACCCTACCGGAAGACGAGTGGGACCCGATGACACTGAACTACACTTCCGGCACTACTTCCTCCCCCAAGGGAGTGGTCCACGGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTTGACTGGGCAGTACCCAGACAGCCGGTCTACCTATGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCAGCAGTCGGTGGTACCAACGTTTGCGTCCGCAAATTCGACGCCCATGTCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACGAATGTACCTAACAACGAGCCTTTGAGGAACCCGGTCCAGATCATGACCTCCGGAGCTCCGCCGCCCGCGGCGGTTCTGTCTCGGACTGAGTCGCTGGGATTCGTGGTGAGCCATGGGTACGGGTTGACAGAGACGGCAGGGCCGGTAGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGATGGTGGCCATGACCCACGTCGACGTGGTGGATCCGGATACGGGTGCGCCGGTTAAACGCGACGGTTCGTCGCTGGGGGAGGTGGTTTTCAGAGGCGGGTCTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACTGCCAGTTGCATGAAAGACGGTTTGTTTTATACCGGCGACGTGGCAGTGATGCACGCGGATGGGTATCTGGAGATCAAGGATCGATCCAAGGACATGATCATTAGCGGGGGAGAGAACGTGAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCCGCGGTGAACGAGGCGGCAGTGGTGGCTAGGCCTGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTGGCGCGGAGGCCGACGGAGAAGGAGATAATAGAGTATTGCAGGGGGAAGTTGCCGCATTACATGGTGCCGAAGACGGTGGTGTTTAAGGAGGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTCGTGCTGAGAGAGATGGCGGCCCAGTGTTACTTGATTCGCGGGTCGTTGCGGAACGGATACACGGTACGGGTACGCAGTATGCCTGTCTCTATGATACGCAGGACGACAGTCTCTATGATACGCAGTACGACAGTCTCTATGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

534

Amino Acids

58.9

Weight (kDa)

8.66

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 13 - 355 5e-65 AMP-binding enzyme
AMP-binding_C PF13193 405 - 482 3.9e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 641
AatII GACGTC 1 cut(s) 1436
Acc65I GGTACC 1 cut(s) 608
AccB1I GGYRCC 3 cut(s) 119, 608, 1393
AccB7I CCANNNNNTGG 1 cut(s) 573
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 4 cut(s) 207, 537, 966, 1436
AccIII TCCGGA 3 cut(s) 373, 767, 979
AclI AACGTT 1 cut(s) 615
AclWI GGATC 6 cut(s) 365, 599, 971, 984, 1161, 1170
AcoI YGGCCR 3 cut(s) 46, 469, 951
AcsI RAATTY 1 cut(s) 629
AcuI CTGAAG 1 cut(s) 944
AcyI GRCGYC 5 cut(s) 120, 636, 672, 1295, 1433
AfiI CCNNNNNNNGG 4 cut(s) 116, 514, 573, 986
AflIII ACRYGT 1 cut(s) 681
AhdI GACNNNNNGTC 3 cut(s) 533, 671, 1012
AjiI CACGTC 5 cut(s) 52, 964, 970, 1123, 1327
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 7 cut(s) 94, 196, 222, 579, 773, 1322, 1423
AluI AGCT 7 cut(s) 94, 196, 222, 579, 773, 1322, 1423
Alw21I GWGCWC 2 cut(s) 96, 775
Alw26I GTCTC 8 cut(s) 61, 169, 801, 842, 1286, 1549, 1573, 1597
AlwI GGATC 6 cut(s) 365, 599, 971, 984, 1161, 1170
Ama87I CYCGRG 1 cut(s) 884
Aor13HI TCCGGA 3 cut(s) 373, 767, 979
AoxI GGCC 7 cut(s) 46, 469, 857, 951, 1271, 1337, 1476
ApeKI GCWGC 3 cut(s) 596, 1206, 1423
ApoI RAATTY 1 cut(s) 629
Asp718I GGTACC 1 cut(s) 608
AspLEI GCGC 7 cut(s) 122, 341, 694, 875, 994, 1304, 1333
AspS9I GGNCC 7 cut(s) 387, 409, 463, 700, 751, 857, 1477
AsuC2I CCSGG 3 cut(s) 749, 885, 886
AsuHPI GGTGA 3 cut(s) 484, 835, 1258
AvaI CYCGRG 1 cut(s) 884
AvaII GGWCC 5 cut(s) 387, 409, 463, 700, 751
BaeI ACNNNNGTAYC 4 cut(s) 600, 633, 705, 738
BalI TGGCCA 1 cut(s) 953
BamHI GGATCC 1 cut(s) 976
BanI GGYRCC 3 cut(s) 119, 608, 1393
BanII GRGCYC 3 cut(s) 96, 483, 775
BbsI GAAGAC 3 cut(s) 405, 1406, 1436
Bbv12I GWGCWC 2 cut(s) 96, 775
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 3 cut(s) 608, 1218, 1410
BccI CCATC 7 cut(s) 30, 143, 252, 315, 940, 1136, 1465
BceAI ACGGC 7 cut(s) 89, 107, 484, 586, 685, 867, 1282
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciVI GTATCC 2 cut(s) 976, 1508
BclI TGATCA 2 cut(s) 657, 1179
BcnI CCSGG 3 cut(s) 749, 885, 886
BcoDI GTCTC 8 cut(s) 61, 169, 801, 842, 1286, 1549, 1573, 1597
BfaI CTAG 1 cut(s) 1269
BfoI RGCGCY 1 cut(s) 123
BfuI GTATCC 2 cut(s) 976, 1508
Bme1390I CCNGG 3 cut(s) 749, 885, 886
Bme18I GGWCC 5 cut(s) 387, 409, 463, 700, 751
BmeRI GACNNNNNGTC 3 cut(s) 533, 671, 1012
BmeT110I CYCGRG 1 cut(s) 884
BmgBI CACGTC 5 cut(s) 52, 964, 970, 1123, 1327
BmgT120I GGNCC 7 cut(s) 387, 409, 463, 700, 751, 857, 1477
BmiI GGNNCC 8 cut(s) 121, 389, 410, 411, 610, 746, 978, 1395
BmrFI CCNGG 3 cut(s) 749, 885, 886
BmrI ACTGGG 2 cut(s) 523, 1474
BmsI GCATC 4 cut(s) 46, 226, 289, 1122
BmuI ACTGGG 2 cut(s) 523, 1474
BoxI GACNNNNGTC 2 cut(s) 962, 1565
BpiI GAAGAC 3 cut(s) 405, 1406, 1436
BpmI CTGGAG 2 cut(s) 595, 1172
Bpu10I CCTNAGC 1 cut(s) 218
BpuMI CCSGG 3 cut(s) 749, 885, 886
Bsa29I ATCGAT 1 cut(s) 1165
BsaAI YACGTR 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 1146
BsaHI GRCGYC 5 cut(s) 120, 636, 672, 1295, 1433
BsaJI CCNNGG 8 cut(s) 32, 453, 466, 783, 829, 884, 1170, 1242
BsaWI WCCGGW 4 cut(s) 373, 394, 767, 979
BsaXI ACNNNNNCTCC 8 cut(s) 41, 71, 1185, 1205, 1215, 1235, 1283, 1313
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 514, 573, 986
Bse118I RCCGGY 6 cut(s) 532, 694, 859, 901, 994, 1115
Bse1I ACTGG 4 cut(s) 382, 518, 1086, 1480
Bse8I GATNNNNATC 1 cut(s) 1146
BseAI TCCGGA 3 cut(s) 373, 767, 979
BseCI ATCGAT 1 cut(s) 1165
BseDI CCNNGG 8 cut(s) 32, 453, 466, 783, 829, 884, 1170, 1242
BseGI GGATG 5 cut(s) 75, 307, 326, 951, 1147
BseJI GATNNNNATC 1 cut(s) 1146
BseLI CCNNNNNNNGG 4 cut(s) 116, 514, 573, 986
BseMII CTCAG 3 cut(s) 232, 795, 1454
BseNI ACTGG 4 cut(s) 382, 518, 1086, 1480
BseRI GAGGAG 7 cut(s) 17, 161, 164, 188, 266, 307, 1433
BseXI GCAGC 3 cut(s) 608, 1218, 1410
BseYI CCCAGC 2 cut(s) 811, 1018
BshFI GGCC 7 cut(s) 48, 471, 859, 953, 1273, 1339, 1478
BshNI GGYRCC 3 cut(s) 119, 608, 1393
BshVI ATCGAT 1 cut(s) 1165
BsiHKAI GWGCWC 2 cut(s) 96, 775
BsiHKCI CYCGRG 1 cut(s) 884
BsiWI CGTACG 2 cut(s) 345, 585
BslFI GGGAC 2 cut(s) 92, 422
BslI CCNNNNNNNGG 4 cut(s) 116, 514, 573, 986
BsmAI GTCTC 8 cut(s) 61, 169, 801, 842, 1286, 1549, 1573, 1597
BsmBI CGTCTC 4 cut(s) 61, 169, 842, 1286
BsmFI GGGAC 2 cut(s) 92, 422
BsnI GGCC 7 cut(s) 48, 471, 859, 953, 1273, 1339, 1478
BsoBI CYCGRG 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 96, 483, 775
Bsp13I TCCGGA 3 cut(s) 373, 767, 979
Bsp143I GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
Bsp19I CCATGG 1 cut(s) 829
BspANI GGCC 7 cut(s) 48, 471, 859, 953, 1273, 1339, 1478
BspCNI CTCAG 3 cut(s) 231, 796, 1455
BspDI ATCGAT 1 cut(s) 1165
BspEI TCCGGA 3 cut(s) 373, 767, 979
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 8 cut(s) 121, 389, 410, 411, 610, 746, 978, 1395
BspPI GGATC 6 cut(s) 365, 599, 971, 984, 1161, 1170
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 3 cut(s) 119, 608, 1393
BsrBI CCGCTC 1 cut(s) 366
BsrFI RCCGGY 6 cut(s) 532, 694, 859, 901, 994, 1115
BsrI ACTGG 4 cut(s) 382, 518, 1086, 1480
BssAI RCCGGY 6 cut(s) 532, 694, 859, 901, 994, 1115
BssECI CCNNGG 8 cut(s) 32, 453, 466, 783, 829, 884, 1170, 1242
BssMI GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
BssNI GRCGYC 5 cut(s) 120, 636, 672, 1295, 1433
BssT1I CCWWGG 3 cut(s) 453, 829, 1170
Bst4CI ACNGT 8 cut(s) 163, 496, 1010, 1103, 1405, 1522, 1567, 1591
Bst6I CTCTTC 3 cut(s) 206, 254, 488
BstACI GRCGYC 5 cut(s) 120, 636, 672, 1295, 1433
BstBAI YACGTR 1 cut(s) 345
BstC8I GCNNGC 3 cut(s) 783, 903, 1137
BstDEI CTNAG 3 cut(s) 218, 804, 1463
BstDSI CCRYGG 4 cut(s) 466, 783, 829, 1242
BstF5I GGATG 5 cut(s) 75, 307, 326, 951, 1147
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 7 cut(s) 122, 341, 694, 875, 994, 1304, 1333
BstKTI GATC 9 cut(s) 373, 594, 660, 759, 979, 1158, 1165, 1169, 1182
BstMAI GTCTC 8 cut(s) 61, 169, 801, 842, 1286, 1549, 1573, 1597
BstMBI GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
BstMWI GCNNNNNNNGC 2 cut(s) 1265, 1328
BstNSI RCATGY 2 cut(s) 685, 712
BstPAI GACNNNNGTC 2 cut(s) 962, 1565
BstSCI CCNGG 3 cut(s) 747, 883, 884
BstV1I GCAGC 3 cut(s) 608, 1218, 1410
BstV2I GAAGAC 3 cut(s) 405, 1406, 1436
BstX2I RGATCY 1 cut(s) 976
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 1 cut(s) 976
Bsu15I ATCGAT 1 cut(s) 1165
BsuI GTATCC 2 cut(s) 976, 1508
BsuRI GGCC 7 cut(s) 48, 471, 859, 953, 1273, 1339, 1478
BsuTUI ATCGAT 1 cut(s) 1165
BtgI CCRYGG 4 cut(s) 466, 783, 829, 1242
BtgZI GCGATG 1 cut(s) 271
BtrI CACGTC 5 cut(s) 52, 964, 970, 1123, 1327
BtsCI GGATG 5 cut(s) 75, 307, 326, 951, 1147
BtsI GCAGTG 3 cut(s) 1080, 1134, 1266
BtsIMutI CAGTG 6 cut(s) 389, 419, 1080, 1134, 1266, 1487
Cac8I GCNNGC 3 cut(s) 783, 903, 1137
CciI TCATGA 1 cut(s) 759
CfoI GCGC 7 cut(s) 122, 341, 694, 875, 994, 1304, 1333
Cfr10I RCCGGY 6 cut(s) 532, 694, 859, 901, 994, 1115
Cfr13I GGNCC 7 cut(s) 387, 409, 463, 700, 751, 857, 1477
Cfr42I CCGCGG 2 cut(s) 786, 1245
Cfr9I CCCGGG 1 cut(s) 884
ClaI ATCGAT 1 cut(s) 1165
CseI GACGC 6 cut(s) 51, 556, 610, 644, 661, 1303
DdeI CTNAG 3 cut(s) 218, 804, 1463
DinI GGCGCC 1 cut(s) 121
DpnI GATC 9 cut(s) 372, 593, 659, 758, 978, 1157, 1164, 1168, 1181
DpnII GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
DrdI GACNNNNNNGTC 1 cut(s) 641
DriI GACNNNNNGTC 3 cut(s) 533, 671, 1012
DseDI GACNNNNNNGTC 1 cut(s) 641
EaeI YGGCCR 3 cut(s) 46, 469, 951
Eam1104I CTCTTC 3 cut(s) 206, 254, 488
Eam1105I GACNNNNNGTC 3 cut(s) 533, 671, 1012
EarI CTCTTC 3 cut(s) 206, 254, 488
EciI GGCGGA 2 cut(s) 167, 765
Ecl136II GAGCTC 2 cut(s) 94, 773
Eco130I CCWWGG 3 cut(s) 453, 829, 1170
Eco147I AGGCCT 1 cut(s) 1273
Eco24I GRGCYC 3 cut(s) 96, 483, 775
Eco47I GGWCC 5 cut(s) 387, 409, 463, 700, 751
Eco53kI GAGCTC 2 cut(s) 94, 773
Eco57I CTGAAG 1 cut(s) 944
Eco88I CYCGRG 1 cut(s) 884
EcoICRI GAGCTC 2 cut(s) 94, 773
EcoO109I RGGNCCY 1 cut(s) 409
EcoT14I CCWWGG 3 cut(s) 453, 829, 1170
EcoT38I GRGCYC 3 cut(s) 96, 483, 775
EgeI GGCGCC 1 cut(s) 121
EheI GGCGCC 1 cut(s) 121
ErhI CCWWGG 3 cut(s) 453, 829, 1170
Esp3I CGTCTC 4 cut(s) 61, 169, 842, 1286
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FaqI GGGAC 2 cut(s) 92, 422
FauI CCCGC 7 cut(s) 88, 152, 164, 790, 1034, 1181, 1490
FbaI TGATCA 2 cut(s) 657, 1179
FblI GTMKAC 4 cut(s) 207, 537, 966, 1436
FokI GGATG 5 cut(s) 62, 294, 313, 958, 1154
FriOI GRGCYC 3 cut(s) 96, 483, 775
FspBI CTAG 1 cut(s) 1269
GlaI GCGC 7 cut(s) 121, 340, 693, 874, 993, 1303, 1332
GsaI CCCAGC 2 cut(s) 815, 1022
GsuI CTGGAG 2 cut(s) 595, 1172
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 7 cut(s) 48, 471, 859, 953, 1273, 1339, 1478
HgaI GACGC 6 cut(s) 51, 556, 610, 644, 661, 1303
HhaI GCGC 7 cut(s) 122, 341, 694, 875, 994, 1304, 1333
Hin1I GRCGYC 5 cut(s) 120, 636, 672, 1295, 1433
Hin6I GCGC 7 cut(s) 120, 339, 692, 873, 992, 1302, 1331
HinP1I GCGC 7 cut(s) 120, 339, 692, 873, 992, 1302, 1331
HincII GTYRAC 5 cut(s) 208, 499, 843, 967, 1437
HindII GTYRAC 5 cut(s) 208, 499, 843, 967, 1437
HinfI GANTC 6 cut(s) 323, 806, 816, 1220, 1447, 1492
HphI GGTGA 3 cut(s) 484, 835, 1258
Hpy166II GTNNAC 9 cut(s) 208, 387, 466, 499, 538, 843, 967, 1249, 1437
Hpy188I TCNGA 7 cut(s) 322, 555, 662, 801, 1037, 1065, 1452
Hpy188III TCNNGA 6 cut(s) 374, 754, 760, 768, 980, 1151
Hpy8I GTNNAC 9 cut(s) 208, 387, 466, 499, 538, 843, 967, 1249, 1437
HpyAV CCTTC 2 cut(s) 343, 1342
HpyCH4III ACNGT 8 cut(s) 163, 496, 1010, 1103, 1405, 1522, 1567, 1591
HpyCH4IV ACGT 9 cut(s) 51, 344, 615, 963, 969, 1122, 1200, 1326, 1433
HpyCH4V TGCA 4 cut(s) 302, 1092, 1135, 1368
HpyF10VI GCNNNNNNNGC 2 cut(s) 1265, 1328
HpyF3I CTNAG 3 cut(s) 218, 804, 1463
HpySE526I ACGT 9 cut(s) 51, 344, 615, 963, 969, 1122, 1200, 1326, 1433
Hsp92I GRCGYC 5 cut(s) 120, 636, 672, 1295, 1433
HspAI GCGC 7 cut(s) 120, 339, 692, 873, 992, 1302, 1331
KasI GGCGCC 1 cut(s) 119
KflI GGGWCCC 1 cut(s) 409
Kpn2I TCCGGA 3 cut(s) 373, 767, 979
KpnI GGTACC 1 cut(s) 612
KroI GCCGGC 1 cut(s) 901
KroNI GCCGGC 1 cut(s) 903
Ksp22I TGATCA 2 cut(s) 657, 1179
KspI CCGCGG 2 cut(s) 786, 1245
Kzo9I GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 7 cut(s) 99, 201, 227, 576, 770, 778, 1420
Lsp1109I GCAGC 3 cut(s) 608, 1218, 1410
LweI GCATC 4 cut(s) 46, 226, 289, 1122
MaeI CTAG 1 cut(s) 1269
MaeII ACGT 9 cut(s) 51, 344, 615, 963, 969, 1122, 1200, 1326, 1433
MaeIII GTNAC 2 cut(s) 673, 1484
MalI GATC 9 cut(s) 372, 593, 659, 758, 978, 1157, 1164, 1168, 1181
MbiI CCGCTC 1 cut(s) 366
MboI GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
MboII GAAGA 7 cut(s) 193, 241, 410, 475, 1411, 1441, 1456
MflI RGATCY 1 cut(s) 976
MhlI GDGCHC 3 cut(s) 96, 483, 775
MlsI TGGCCA 1 cut(s) 953
MluCI AATT 1 cut(s) 629
MluNI TGGCCA 1 cut(s) 953
Mly113I GGCGCC 1 cut(s) 120
MlyI GAGTC 3 cut(s) 317, 815, 1229
MmeI TCCRAC 1 cut(s) 345
Mox20I TGGCCA 1 cut(s) 953
MroI TCCGGA 3 cut(s) 373, 767, 979
MroNI GCCGGC 1 cut(s) 901
MscI TGGCCA 1 cut(s) 953
MseI TTAA 2 cut(s) 999, 1413
MslI CAYNNNNRTG 2 cut(s) 680, 1140
Msp20I TGGCCA 1 cut(s) 953
MspA1I CMGCKG 2 cut(s) 785, 1244
MspR9I CCNGG 3 cut(s) 749, 885, 886
MwoI GCNNNNNNNGC 2 cut(s) 1265, 1328
NaeI GCCGGC 1 cut(s) 903
NarI GGCGCC 1 cut(s) 120
NciI CCSGG 3 cut(s) 749, 885, 886
NcoI CCATGG 1 cut(s) 829
NdeII GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
NgoMIV GCCGGC 1 cut(s) 901
NlaIV GGNNCC 8 cut(s) 121, 389, 410, 411, 610, 746, 978, 1395
NmeAIII GCCGAG 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 673
NspI RCATGY 2 cut(s) 685, 712
PagI TCATGA 1 cut(s) 759
PceI AGGCCT 1 cut(s) 1273
PciI ACATGT 1 cut(s) 681
PciSI GCTCTTC 1 cut(s) 488
PcsI WCGNNNNNNNCGW 4 cut(s) 57, 914, 1206, 1301
PdiI GCCGGC 1 cut(s) 903
PfeI GAWTC 3 cut(s) 816, 1447, 1492
Pfl23II CGTACG 2 cut(s) 345, 585
PflFI GACNNNGTC 1 cut(s) 805
PflMI CCANNNNNTGG 1 cut(s) 573
PleI GAGTC 3 cut(s) 317, 814, 1228
PluTI GGCGCC 1 cut(s) 123
PpsI GAGTC 3 cut(s) 317, 814, 1228
Ppu21I YACGTR 1 cut(s) 345
PpuMI RGGWCCY 1 cut(s) 409
PscI ACATGT 1 cut(s) 681
PshAI GACNNNNGTC 2 cut(s) 962, 1565
Psp124BI GAGCTC 2 cut(s) 96, 775
Psp1406I AACGTT 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 409
PspFI CCCAGC 2 cut(s) 811, 1018
PspLI CGTACG 2 cut(s) 345, 585
PspN4I GGNNCC 8 cut(s) 121, 389, 410, 411, 610, 746, 978, 1395
PspPI GGNCC 7 cut(s) 387, 409, 463, 700, 751, 857, 1477
PspPPI RGGWCCY 1 cut(s) 409
PsuI RGATCY 1 cut(s) 976
PsyI GACNNNGTC 1 cut(s) 805
RseI CAYNNNNRTG 2 cut(s) 680, 1140
SacI GAGCTC 2 cut(s) 96, 775
SacII CCGCGG 2 cut(s) 786, 1245
SalI GTCGAC 3 cut(s) 206, 965, 1435
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 2 cut(s) 999, 1413
Sau3AI GATC 9 cut(s) 370, 591, 657, 756, 976, 1155, 1162, 1166, 1179
Sau96I GGNCC 7 cut(s) 387, 409, 463, 700, 751, 857, 1477
SchI GAGTC 3 cut(s) 317, 815, 1229
ScrFI CCNGG 3 cut(s) 749, 885, 886
SduI GDGCHC 3 cut(s) 96, 483, 775
SfaNI GCATC 4 cut(s) 46, 226, 289, 1122
SfoI GGCGCC 1 cut(s) 121
Sfr303I CCGCGG 2 cut(s) 786, 1245
SgrAI CRCCGGYG 1 cut(s) 694
SgrBI CCGCGG 2 cut(s) 786, 1245
SgrDI CGTCGACG 2 cut(s) 965, 1435
SinI GGWCC 5 cut(s) 387, 409, 463, 700, 751
SmaI CCCGGG 1 cut(s) 886
SmiMI CAYNNNNRTG 2 cut(s) 680, 1140
Sse9I AATT 1 cut(s) 629
SseBI AGGCCT 1 cut(s) 1273
SspDI GGCGCC 1 cut(s) 119
SspMI CTAG 1 cut(s) 1269
SstI GAGCTC 2 cut(s) 96, 775
StuI AGGCCT 1 cut(s) 1273
StyD4I CCNGG 3 cut(s) 747, 883, 884
StyI CCWWGG 3 cut(s) 453, 829, 1170
TaaI ACNGT 8 cut(s) 163, 496, 1010, 1103, 1405, 1522, 1567, 1591
TaiI ACGT 9 cut(s) 54, 347, 618, 966, 972, 1125, 1203, 1329, 1436
TasI AATT 1 cut(s) 629
TauI GCSGC 8 cut(s) 48, 369, 781, 788, 1244, 1259, 1384, 1478
TfiI GAWTC 3 cut(s) 816, 1447, 1492
Tru1I TTAA 2 cut(s) 999, 1413
Tru9I TTAA 2 cut(s) 999, 1413
TscAI CASTG 6 cut(s) 389, 426, 1087, 1134, 1266, 1487
TseFI GTSAC 1 cut(s) 673
TseI GCWGC 3 cut(s) 596, 1206, 1423
Tsp45I GTSAC 1 cut(s) 673
TspDTI ATGAA 2 cut(s) 475, 1109
TspGWI ACGGA 4 cut(s) 49, 923, 1358, 1527
TspMI CCCGGG 1 cut(s) 884
TspRI CASTG 6 cut(s) 389, 426, 1087, 1134, 1266, 1487
Tth111I GACNNNGTC 1 cut(s) 805
Van91I CCANNNNNTGG 1 cut(s) 573
VpaK11BI GGWCC 5 cut(s) 387, 409, 463, 700, 751
XapI RAATTY 1 cut(s) 629
XceI RCATGY 2 cut(s) 685, 712
XmaI CCCGGG 1 cut(s) 884
XmiI GTMKAC 4 cut(s) 207, 537, 966, 1436
XspI CTAG 1 cut(s) 1269
ZraI GACGTC 1 cut(s) 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.