Rmu_sc0001491.1_g000005

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001491.1
OG0000677
Physical Location & Seq
Forward (+)
26284 .. 28041
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001491.1_g000005.1.cds

Sequence Viewer

Length: 828 bp
atgacccacgtcgacgtggtggatccgaatacgggtgcgccggttaaaagcgacggttcatcgctcggggaggtggttttcaaaggcgggtctgtgatgttgggttacctgaaagacccaaaaggcacggctagttgcatgaaagacggtttgttttataccggcgacgtggcagtgatgcacccggacgggtatctggagatcaaggatcgatctaaggatgtgatcattagcgggggagagaacgtgagcagcatggaggtcgagtcggttctctacaccaacgcggcggtgaacgaggctgcagtggtggctaggcctgacgagttttggggggagacgccgtgcgcgtttgtgagcctgaaagctgacgtggcgcagaggccgacggagaaggagataatagaatattgcagggggaagttgccgcattacatggtgccgaagacggtggtgtttaaggaggagctgccgaagacttcgacggggaagattcagaagttcgtgctgagagagatggcggcccagtgttacttggttcgcatgtcgttgcggaatgggtatgaacgaagaaacacattaggtttggatcattctgatgttaccatgcaaggcatggctgaggaggagtcatgtgagcctgctgcaatgaagcagccagcagagactatgtatgatgtggaggcctcgggcttggcctccatgaatggtgttttgaatttcattcctcctactaaggttgtgcagattgaacctaaggtttacaagaaaagaagcaggaatgaagaagccataatttcagaatgccactattaccagaaccagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

275

Amino Acids

30.4

Weight (kDa)

5.3

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 12
AccII CGCG 2 cut(s) 287, 350
AciI CCGC 7 cut(s) 87, 234, 287, 290, 428, 521, 553
AclWI GGATC 4 cut(s) 17, 30, 216, 597
AcsI RAATTY 1 cut(s) 718
AcyI GRCGYC 1 cut(s) 341
AfiI CCNNNNNNNGG 1 cut(s) 32
AgsI TTSAA 3 cut(s) 82, 718, 752
AjiI CACGTC 4 cut(s) 10, 16, 169, 373
AluBI AGCT 2 cut(s) 368, 469
AluI AGCT 2 cut(s) 368, 469
Alw26I GTCTC 2 cut(s) 332, 659
AlwI GGATC 4 cut(s) 17, 30, 216, 597
Ama87I CYCGRG 2 cut(s) 65, 688
AoxI GGCC 5 cut(s) 317, 383, 522, 684, 696
ApeKI GCWGC 5 cut(s) 252, 302, 469, 644, 655
ApoI RAATTY 1 cut(s) 718
AspLEI GCGC 3 cut(s) 40, 350, 379
AspS9I GGNCC 1 cut(s) 523
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 2 cut(s) 65, 688
AxyI CCTNAGG 1 cut(s) 756
BamHI GGATCC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 452, 482
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 5 cut(s) 264, 289, 456, 631, 667
BccI CCATC 1 cut(s) 511
BceAI ACGGC 2 cut(s) 144, 328
BcgI CGANNNNNNTGC 2 cut(s) 244, 278
BclI TGATCA 1 cut(s) 225
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 2 cut(s) 332, 659
BfaI CTAG 2 cut(s) 132, 315
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 8 cut(s) 253, 288, 303, 428, 470, 522, 645, 656
BlsI GCNGC 8 cut(s) 254, 289, 304, 429, 471, 523, 646, 657
Bme1390I CCNGG 1 cut(s) 185
BmeT110I CYCGRG 2 cut(s) 65, 688
BmgBI CACGTC 4 cut(s) 10, 16, 169, 373
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 2 cut(s) 24, 441
BmrFI CCNGG 1 cut(s) 185
BmrI ACTGGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 520
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 452, 482
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 185
Bsa29I ATCGAT 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 687
BsaXI ACNNNNNCTCC 6 cut(s) 231, 251, 261, 281, 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 2 cut(s) 40, 161
Bse1I ACTGG 2 cut(s) 526, 823
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 654
BseCI ATCGAT 1 cut(s) 211
BseDI CCNNGG 1 cut(s) 687
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 2 cut(s) 500, 612
BseNI ACTGG 2 cut(s) 526, 823
BseRI GAGGAG 3 cut(s) 479, 638, 641
BseXI GCAGC 5 cut(s) 264, 289, 456, 631, 667
BsgI GTGCAG 1 cut(s) 764
Bsh1236I CGCG 2 cut(s) 287, 350
BshFI GGCC 5 cut(s) 319, 385, 524, 686, 698
BshNI GGYRCC 1 cut(s) 439
BshVI ATCGAT 1 cut(s) 211
BsiHKCI CYCGRG 2 cut(s) 65, 688
BsiSI CCGG 3 cut(s) 41, 162, 185
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 2 cut(s) 332, 659
BsmBI CGTCTC 1 cut(s) 332
BsmI GAATGC 1 cut(s) 809
BsnI GGCC 5 cut(s) 319, 385, 524, 686, 698
BsoBI CYCGRG 2 cut(s) 65, 688
Bsp143I GATC 6 cut(s) 22, 201, 208, 212, 225, 589
BspACI CCGC 7 cut(s) 87, 234, 287, 290, 428, 521, 553
BspANI GGCC 5 cut(s) 319, 385, 524, 686, 698
BspCNI CTCAG 2 cut(s) 501, 613
BspDI ATCGAT 1 cut(s) 211
BspFNI CGCG 2 cut(s) 287, 350
BspLI GGNNCC 2 cut(s) 24, 441
BspMAI CTGCAG 1 cut(s) 307
BspPI GGATC 4 cut(s) 17, 30, 216, 597
BspT107I GGYRCC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 654
BsrFI RCCGGY 2 cut(s) 40, 161
BsrI ACTGG 2 cut(s) 526, 823
BssAI RCCGGY 2 cut(s) 40, 161
BssECI CCNNGG 1 cut(s) 687
BssMI GATC 6 cut(s) 22, 201, 208, 212, 225, 589
BssNI GRCGYC 1 cut(s) 341
Bst4CI ACNGT 3 cut(s) 56, 149, 451
BstACI GRCGYC 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 642, 660
BstDEI CTNAG 5 cut(s) 216, 509, 621, 735, 756
BstEII GGTNACC 1 cut(s) 104
BstF5I GGATG 1 cut(s) 226
BstFNI CGCG 2 cut(s) 287, 350
BstHHI GCGC 3 cut(s) 40, 350, 379
BstKTI GATC 6 cut(s) 25, 204, 211, 215, 228, 592
BstMAI GTCTC 2 cut(s) 332, 659
BstMBI GATC 6 cut(s) 22, 201, 208, 212, 225, 589
BstMWI GCNNNNNNNGC 2 cut(s) 311, 374
BstNSI RCATGY 1 cut(s) 547
BstPAI GACNNNNGTC 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 303
BstUI CGCG 2 cut(s) 287, 350
BstV1I GCAGC 5 cut(s) 264, 289, 456, 631, 667
BstV2I GAAGAC 2 cut(s) 452, 482
BstX2I RGATCY 1 cut(s) 22
BstYI RGATCY 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 211
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 5 cut(s) 319, 385, 524, 686, 698
BsuTUI ATCGAT 1 cut(s) 211
BtgZI GCGATG 1 cut(s) 45
BtrI CACGTC 4 cut(s) 10, 16, 169, 373
BtsCI GGATG 1 cut(s) 226
BtsI GCAGTG 2 cut(s) 180, 312
BtsIMutI CAGTG 3 cut(s) 180, 312, 533
Cac8I GCNNGC 2 cut(s) 642, 660
CfoI GCGC 3 cut(s) 40, 350, 379
Cfr10I RCCGGY 2 cut(s) 40, 161
Cfr13I GGNCC 1 cut(s) 523
ClaI ATCGAT 1 cut(s) 211
CseI GACGC 1 cut(s) 349
CviAII CATG 8 cut(s) 139, 256, 436, 544, 607, 616, 633, 703
DdeI CTNAG 5 cut(s) 216, 509, 621, 735, 756
DpnI GATC 6 cut(s) 24, 203, 210, 214, 227, 591
DpnII GATC 6 cut(s) 22, 201, 208, 212, 225, 589
Eco147I AGGCCT 2 cut(s) 319, 686
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 2 cut(s) 65, 688
Eco91I GGTNACC 1 cut(s) 104
EcoO65I GGTNACC 1 cut(s) 104
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 8 cut(s) 142, 259, 439, 547, 610, 619, 636, 706
FatI CATG 8 cut(s) 138, 255, 435, 543, 606, 615, 632, 702
FauI CCCGC 2 cut(s) 80, 227
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 8 cut(s) 253, 288, 303, 428, 470, 522, 645, 656
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 8 cut(s) 253, 288, 303, 428, 470, 522, 645, 656
FspBI CTAG 2 cut(s) 132, 315
GlaI GCGC 3 cut(s) 39, 349, 378
GluI GCNGC 8 cut(s) 253, 288, 303, 428, 470, 522, 645, 656
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 5 cut(s) 319, 385, 524, 686, 698
HapII CCGG 3 cut(s) 41, 162, 185
HgaI GACGC 1 cut(s) 349
HhaI GCGC 3 cut(s) 40, 350, 379
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 8 cut(s) 142, 259, 439, 547, 610, 619, 636, 706
Hin6I GCGC 3 cut(s) 38, 348, 377
HinP1I GCGC 3 cut(s) 38, 348, 377
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 3 cut(s) 266, 493, 629
HpaII CCGG 3 cut(s) 41, 162, 185
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 3 cut(s) 13, 295, 763
Hpy188I TCNGA 4 cut(s) 27, 498, 598, 802
Hpy188III TCNNGA 1 cut(s) 197
Hpy8I GTNNAC 3 cut(s) 13, 295, 763
Hpy99I CGWCG 6 cut(s) 14, 17, 56, 170, 391, 487
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 3 cut(s) 56, 149, 451
HpyCH4IV ACGT 5 cut(s) 9, 15, 168, 246, 372
HpyCH4V TGCA 7 cut(s) 138, 181, 305, 414, 610, 647, 745
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 374
HpyF3I CTNAG 5 cut(s) 216, 509, 621, 735, 756
HpySE526I ACGT 5 cut(s) 9, 15, 168, 246, 372
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 8 cut(s) 142, 259, 439, 547, 610, 619, 636, 706
HspAI GCGC 3 cut(s) 38, 348, 377
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 6 cut(s) 22, 201, 208, 212, 225, 589
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 5 cut(s) 264, 289, 456, 631, 667
LweI GCATC 1 cut(s) 168
MaeI CTAG 2 cut(s) 132, 315
MaeII ACGT 5 cut(s) 9, 15, 168, 246, 372
MaeIII GTNAC 3 cut(s) 104, 530, 601
MalI GATC 6 cut(s) 24, 203, 210, 214, 227, 591
MboI GATC 6 cut(s) 22, 201, 208, 212, 225, 589
MboII GAAGA 5 cut(s) 457, 487, 502, 582, 797
MflI RGATCY 1 cut(s) 22
MluCI AATT 2 cut(s) 718, 795
MlyI GAGTC 2 cut(s) 275, 638
MseI TTAA 2 cut(s) 45, 459
MslI CAYNNNNRTG 1 cut(s) 597
MspI CCGG 3 cut(s) 41, 162, 185
MspR9I CCNGG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 809
MvnI CGCG 2 cut(s) 287, 350
MwoI GCNNNNNNNGC 2 cut(s) 311, 374
NciI CCSGG 1 cut(s) 185
NdeII GATC 6 cut(s) 22, 201, 208, 212, 225, 589
NlaIII CATG 8 cut(s) 142, 259, 439, 547, 610, 619, 636, 706
NlaIV GGNNCC 2 cut(s) 24, 441
NspI RCATGY 1 cut(s) 547
PceI AGGCCT 2 cut(s) 319, 686
PcsI WCGNNNNNNNCGW 1 cut(s) 347
PctI GAATGC 1 cut(s) 809
PfeI GAWTC 1 cut(s) 493
PkrI GCNGC 8 cut(s) 254, 289, 304, 429, 471, 523, 646, 657
PleI GAGTC 2 cut(s) 274, 637
PpsI GAGTC 2 cut(s) 274, 637
PshAI GACNNNNGTC 1 cut(s) 8
PspEI GGTNACC 1 cut(s) 104
PspN4I GGNNCC 2 cut(s) 24, 441
PspPI GGNCC 1 cut(s) 523
PstI CTGCAG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 597
SalI GTCGAC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 45, 459
SatI GCNGC 8 cut(s) 253, 288, 303, 428, 470, 522, 645, 656
Sau3AI GATC 6 cut(s) 22, 201, 208, 212, 225, 589
Sau96I GGNCC 1 cut(s) 523
SchI GAGTC 2 cut(s) 275, 638
ScrFI CCNGG 1 cut(s) 185
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 1 cut(s) 303
SgrDI CGTCGACG 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 597
Sse9I AATT 2 cut(s) 718, 795
SseBI AGGCCT 2 cut(s) 319, 686
SsiI CCGC 7 cut(s) 87, 234, 287, 290, 428, 521, 553
SspI AATATT 1 cut(s) 410
SspMI CTAG 2 cut(s) 132, 315
StuI AGGCCT 2 cut(s) 319, 686
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 3 cut(s) 56, 149, 451
TaiI ACGT 5 cut(s) 12, 18, 171, 249, 375
TaqI TCGA 4 cut(s) 12, 211, 264, 482
TasI AATT 2 cut(s) 718, 795
TauI GCSGC 3 cut(s) 290, 430, 524
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 2 cut(s) 45, 459
Tru9I TTAA 2 cut(s) 45, 459
TscAI CASTG 3 cut(s) 180, 312, 533
TseI GCWGC 5 cut(s) 252, 302, 469, 644, 655
TspDTI ATGAA 7 cut(s) 48, 155, 579, 665, 712, 719, 798
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 3 cut(s) 180, 312, 533
XapI RAATTY 1 cut(s) 718
XceI RCATGY 1 cut(s) 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 2 cut(s) 132, 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.