AT1G21540

ligase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000677
Physical Location & Seq
Reverse (-)
7548450 .. 7550741
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21540.1

Sequence Viewer

Length: 1653 bp
ATGGAACTCTTACTTCCACACCCTTCGAACTCAACACCTCTCACCGTCCTCGGCTTCTTAGACCGAGCCGCCTCTGTCTACGGCGACTGTCCGTCCATCCTCCACACCACTAACACCGTCCACACTTGGTCCGAAACCCACAACCGCTGTCTTCGAATCGCCTCGGCTCTCACTTCTTCCTCTCTCGGCATAAACCGAGGCCAAGTTGTCTCCGTCGTAGGTCCCAACGTCCCGTCCGTCTACGAGCTTCAGTTTGCTGTCCCAATGTCCGGAGCCATCTTAAACAACATCAACCCTCGTTTGGACGCACATGCACTCTCTGTCCTCCTGCGTCACAGTGAATCTAAGCTCGTTTTCGTCGATCCCAATTCAATCTCCGTAGTCCTCGAAGCAGTCTCGTTCATGAGGCAAAACGAGAAACCTCACCTTGTCCTCCTAGACGACGACCAAGAGGACGGTTCTTTGTCTCCTTCGGCCGCATCAGATTTTCTTGACACATACCAAGGAGTTATGGAGAGAGGAGATTCAAGATTCAAGTGGATCCGTCCTCAAACCGAGTGGCAGCCTATGATTCTCAACTATACTTCTGGAACAACGTCATCTCCCAAAGGAGTAGTGTTGAGCCACAGAGCAATTTTCATGCTAACCGTAAGCTCATTGCTTGACTGGCATTTTCCGAACAGGCCGGTTTACTTGTGGACTCTACCAATGTTCCACGCCAATGGTTGGGGGTATACATGGGGCACTGCGGCGGTTGGAGCCACCAACGTCTGCACCCGTAGAGTCGACGCGCCGACTATCTATGACTTGATCGATAAGCATCACGTAACTCACATGTGTGCCGCACCAATGGTTCTCAACATGCTAACCAACTACCCGTCTCGTAAACCGCTAAAGAACCCGGTTCAGGTTATGACCGCCGGAGCTCCACCTCCAGCAGCCATCATCTCAAGAGCAGAAACCCTAGGTTTCAACGTCGGTCATGGGTACGGTTTAACGGAAACCGGAGGCCCGGTTGTGTCATGTGCTTGGAAGGCTGAGTGGGATCATCTTGATCCATTGGAAAGAGCAAGACTGAAATCAAGACAAGGAGTAAGAACCATCGGATTCGCGGAAGTTGATGTTAGGGACCCGAGAACCGGGAAGAGTGTGGAACACGACGGTGTTTCCGTTGGAGAGATCGTTTTAAAAGGCGGTTCGGTTATGCTTGGTTACTACAAAGACCCTGAAGGAACCGCAGCGTGTATGAGAGAGGACGGATGGTTCTACAGCGGAGACGTAGGAGTTATACATGAAGACGGTTACTTGGAAGTTAAAGACCGGTCAAAGGATGTGATCATATGCGGAGGAGAGAACATAAGTAGTGCCGAGGTTGAGACGGTTCTGTATACAAATCCAGTAGTTAAAGAAGCCGCGGTGGTGGCTAAACCGGATAAGATGTGGGGAGAGACACCGTGTGCTTTTGTGAGCTTGAAGTATGATAGTAATGGTAATGGGTTGGTGACTGAGAGGGAAATAAGGGAGTTTTGTAAGACGAGGTTACCTAAGTATATGGTTCCAAGGAAAGTAATTTTTCAGGAGGAGCTTCCAAAGACTTCTACTGGAAAGATACAGAAGTTTCTTCTAAGACAAATGGCTAAGTCTCTGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

550

Amino Acids

60.63

Weight (kDa)

6.5

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 406 4e-75 AMP-binding enzyme
AMP-binding_C PF13193 457 - 536 1.7e-23 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 726
AccI GTMKAC 5 cut(s) 78, 240, 734, 786, 1388
AccII CGCG 3 cut(s) 791, 1112, 1415
AccIII TCCGGA 1 cut(s) 269
AclWI GGATC 5 cut(s) 356, 535, 548, 1049, 1053
AcoI YGGCCR 1 cut(s) 474
AcuI CTGAAG 2 cut(s) 233, 1248
AdeI CACNNNGTG 1 cut(s) 1457
AfaI GTAC 1 cut(s) 989
AfiI CCNNNNNNNGG 6 cut(s) 269, 301, 726, 907, 1139, 1327
AflIII ACRYGT 1 cut(s) 834
AgeI ACCGGT 1 cut(s) 1320
AgsI TTSAA 5 cut(s) 372, 528, 535, 973, 1474
AhdI GACNNNNNGTC 1 cut(s) 91
AleI CACNNNNGTG 2 cut(s) 837, 1161
AloI GAACNNNNNNTCC 2 cut(s) 1247, 1279
AluBI AGCT 6 cut(s) 247, 349, 654, 926, 1470, 1585
AluI AGCT 6 cut(s) 247, 349, 654, 926, 1470, 1585
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 8 cut(s) 214, 400, 471, 885, 1269, 1370, 1442, 1647
AlwI GGATC 5 cut(s) 356, 535, 548, 1049, 1053
Ama87I CYCGRG 1 cut(s) 1132
Aor13HI TCCGGA 1 cut(s) 269
AoxI GGCC 4 cut(s) 199, 474, 683, 1009
ApeKI GCWGC 3 cut(s) 562, 938, 1238
AsiGI ACCGGT 1 cut(s) 1320
AspA2I CCTAGG 1 cut(s) 964
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 4 cut(s) 129, 221, 1010, 1129
AsuC2I CCSGG 3 cut(s) 902, 1013, 1141
AsuHPI GGTGA 3 cut(s) 34, 416, 1513
AsuII TTCGAA 2 cut(s) 26, 154
AvaI CYCGRG 1 cut(s) 1132
AvaII GGWCC 3 cut(s) 129, 221, 1129
AvrII CCTAGG 1 cut(s) 964
BaeGI GKGCMC 1 cut(s) 746
BaeI ACNNNNGTAYC 2 cut(s) 1601, 1634
BamHI GGATCC 1 cut(s) 540
BanII GRGCYC 1 cut(s) 928
BbsI GAAGAC 2 cut(s) 143, 1302
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 3 cut(s) 574, 950, 1250
BccI CCATC 5 cut(s) 104, 284, 950, 1109, 1254
BceAI ACGGC 1 cut(s) 97
BclI TGATCA 1 cut(s) 1335
BcnI CCSGG 3 cut(s) 902, 1013, 1141
BcoDI GTCTC 8 cut(s) 214, 400, 471, 885, 1269, 1370, 1442, 1647
BfaI CTAG 2 cut(s) 437, 965
BfmI CTRYAG 1 cut(s) 1267
BisI GCNGC 8 cut(s) 69, 477, 563, 750, 843, 939, 1239, 1413
BlnI CCTAGG 1 cut(s) 964
BlsI GCNGC 8 cut(s) 70, 478, 564, 751, 844, 940, 1240, 1414
Bme1390I CCNGG 3 cut(s) 902, 1013, 1141
Bme18I GGWCC 3 cut(s) 129, 221, 1129
BmeRI GACNNNNNGTC 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 1132
BmgT120I GGNCC 4 cut(s) 129, 221, 1010, 1129
BmiI GGNNCC 8 cut(s) 223, 274, 542, 760, 1130, 1131, 1234, 1557
BmrFI CCNGG 3 cut(s) 902, 1013, 1141
BmsI GCATC 2 cut(s) 488, 829
BpiI GAAGAC 2 cut(s) 143, 1302
BpmI CTGGAG 1 cut(s) 918
Bpu14I TTCGAA 2 cut(s) 26, 154
BpuEI CTTGAG 1 cut(s) 934
BpuMI CCSGG 3 cut(s) 902, 1013, 1141
Bsa29I ATCGAT 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 826
BsaBI GATNNNNATC 1 cut(s) 819
BsaJI CCNNGG 8 cut(s) 49, 162, 196, 502, 964, 1368, 1413, 1559
BsaWI WCCGGW 4 cut(s) 269, 1004, 1320, 1429
BsaXI ACNNNNNCTCC 6 cut(s) 586, 616, 999, 1029, 1167, 1197
Bsc4I CCNNNNNNNGG 6 cut(s) 269, 301, 726, 907, 1139, 1327
Bse118I RCCGGY 2 cut(s) 685, 1320
Bse1I ACTGG 3 cut(s) 671, 1397, 1606
Bse3DI GCAATG 1 cut(s) 656
Bse8I GATNNNNATC 1 cut(s) 819
BseAI TCCGGA 1 cut(s) 269
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 8 cut(s) 49, 162, 196, 502, 964, 1368, 1413, 1559
BseGI GGATG 3 cut(s) 96, 1265, 1336
BseJI GATNNNNATC 1 cut(s) 819
BseLI CCNNNNNNNGG 6 cut(s) 269, 301, 726, 907, 1139, 1327
BseMI GCAATG 1 cut(s) 656
BseMII CTCAG 2 cut(s) 1029, 1497
BseNI ACTGG 3 cut(s) 671, 1397, 1606
BseRI GAGGAG 3 cut(s) 534, 1362, 1595
BseSI GKGCMC 1 cut(s) 746
BseX3I CGGCCG 1 cut(s) 474
BseXI GCAGC 3 cut(s) 574, 950, 1250
BsgI GTGCAG 1 cut(s) 757
Bsh1236I CGCG 3 cut(s) 791, 1112, 1415
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 4 cut(s) 201, 476, 685, 1011
BshTI ACCGGT 1 cut(s) 1320
BshVI ATCGAT 1 cut(s) 813
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 928
BsiHKCI CYCGRG 1 cut(s) 1132
BsiSI CCGG 9 cut(s) 270, 686, 902, 921, 1005, 1013, 1140, 1321, 1430
BslFI GGGAC 4 cut(s) 207, 215, 245, 1142
BslI CCNNNNNNNGG 6 cut(s) 269, 301, 726, 907, 1139, 1327
BsmAI GTCTC 8 cut(s) 214, 400, 471, 885, 1269, 1370, 1442, 1647
BsmBI CGTCTC 3 cut(s) 885, 1269, 1370
BsmFI GGGAC 4 cut(s) 207, 215, 245, 1142
BsnI GGCC 4 cut(s) 201, 476, 685, 1011
BsoBI CYCGRG 1 cut(s) 1132
Bsp119I TTCGAA 2 cut(s) 26, 154
Bsp1286I GDGCHC 2 cut(s) 746, 928
Bsp13I TCCGGA 1 cut(s) 269
Bsp143I GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
BspANI GGCC 4 cut(s) 201, 476, 685, 1011
BspCNI CTCAG 2 cut(s) 1030, 1498
BspDI ATCGAT 1 cut(s) 813
BspEI TCCGGA 1 cut(s) 269
BspFNI CGCG 3 cut(s) 791, 1112, 1415
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 8 cut(s) 223, 274, 542, 760, 1130, 1131, 1234, 1557
BspPI GGATC 5 cut(s) 356, 535, 548, 1049, 1053
BspT104I TTCGAA 2 cut(s) 26, 154
BsrDI GCAATG 1 cut(s) 656
BsrFI RCCGGY 2 cut(s) 685, 1320
BsrI ACTGG 3 cut(s) 671, 1397, 1606
BssAI RCCGGY 2 cut(s) 685, 1320
BssECI CCNNGG 8 cut(s) 49, 162, 196, 502, 964, 1368, 1413, 1559
BssMI GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
BssNAI GTATAC 2 cut(s) 735, 1389
BssT1I CCWWGG 3 cut(s) 502, 964, 1559
Bst1107I GTATAC 2 cut(s) 735, 1389
Bst6I CTCTTC 1 cut(s) 1139
BstBAI YACGTR 1 cut(s) 826
BstBI TTCGAA 2 cut(s) 26, 154
BstDEI CTNAG 7 cut(s) 58, 345, 1038, 1506, 1545, 1625, 1638
BstDSI CCRYGG 1 cut(s) 1413
BstEII GGTNACC 1 cut(s) 1539
BstF5I GGATG 3 cut(s) 96, 1265, 1336
BstFNI CGCG 3 cut(s) 791, 1112, 1415
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 7 cut(s) 364, 543, 813, 1048, 1057, 1182, 1338
BstMAI GTCTC 8 cut(s) 214, 400, 471, 885, 1269, 1370, 1442, 1647
BstMBI GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 4 cut(s) 667, 758, 1034, 1421
BstNSI RCATGY 3 cut(s) 314, 838, 865
BstPI GGTNACC 1 cut(s) 1539
BstSCI CCNGG 3 cut(s) 900, 1011, 1139
BstSFI CTRYAG 1 cut(s) 1267
BstSLI GKGCMC 1 cut(s) 746
BstUI CGCG 3 cut(s) 791, 1112, 1415
BstV1I GCAGC 3 cut(s) 574, 950, 1250
BstV2I GAAGAC 2 cut(s) 143, 1302
BstX2I RGATCY 1 cut(s) 540
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 1 cut(s) 540
BstZ17I GTATAC 2 cut(s) 735, 1389
BstZI CGGCCG 1 cut(s) 474
Bsu15I ATCGAT 1 cut(s) 813
BsuRI GGCC 4 cut(s) 201, 476, 685, 1011
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 1413
BtsCI GGATG 3 cut(s) 96, 1265, 1336
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 2 cut(s) 343, 744
CciI TCATGA 1 cut(s) 402
CfoI GCGC 1 cut(s) 793
Cfr10I RCCGGY 2 cut(s) 685, 1320
Cfr13I GGNCC 4 cut(s) 129, 221, 1010, 1129
Cfr42I CCGCGG 1 cut(s) 1416
ClaI ATCGAT 1 cut(s) 813
CseI GACGC 3 cut(s) 314, 320, 797
Csp6I GTAC 1 cut(s) 988
CspAI ACCGGT 1 cut(s) 1320
CspCI CAANNNNNGTGG 2 cut(s) 539, 574
CviAII CATG 9 cut(s) 311, 403, 640, 738, 835, 862, 983, 1023, 1292
CviQI GTAC 1 cut(s) 988
DdeI CTNAG 7 cut(s) 58, 345, 1038, 1506, 1545, 1625, 1638
DpnI GATC 7 cut(s) 363, 542, 812, 1047, 1056, 1181, 1337
DpnII GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
DraI TTTAAA 1 cut(s) 1188
DraIII CACNNNGTG 1 cut(s) 1457
DriI GACNNNNNGTC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 474
EagI CGGCCG 1 cut(s) 474
Eam1104I CTCTTC 1 cut(s) 1139
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 1139
Ecl136II GAGCTC 1 cut(s) 926
EclXI CGGCCG 1 cut(s) 474
Eco130I CCWWGG 3 cut(s) 502, 964, 1559
Eco24I GRGCYC 1 cut(s) 928
Eco47I GGWCC 3 cut(s) 129, 221, 1129
Eco52I CGGCCG 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 926
Eco57I CTGAAG 2 cut(s) 233, 1248
Eco88I CYCGRG 1 cut(s) 1132
Eco91I GGTNACC 1 cut(s) 1539
EcoICRI GAGCTC 1 cut(s) 926
EcoO109I RGGNCCY 2 cut(s) 221, 1129
EcoO65I GGTNACC 1 cut(s) 1539
EcoT14I CCWWGG 3 cut(s) 502, 964, 1559
EcoT38I GRGCYC 1 cut(s) 928
ErhI CCWWGG 3 cut(s) 502, 964, 1559
Esp3I CGTCTC 3 cut(s) 885, 1269, 1370
FaeI CATG 9 cut(s) 314, 406, 643, 741, 838, 865, 986, 1026, 1295
FaqI GGGAC 4 cut(s) 207, 215, 245, 1142
FatI CATG 9 cut(s) 310, 402, 639, 737, 834, 861, 982, 1022, 1291
FauNDI CATATG 1 cut(s) 1340
FbaI TGATCA 1 cut(s) 1335
FblI GTMKAC 5 cut(s) 78, 240, 734, 786, 1388
Fnu4HI GCNGC 8 cut(s) 69, 477, 563, 750, 843, 939, 1239, 1413
FokI GGATG 3 cut(s) 83, 1272, 1343
FriOI GRGCYC 1 cut(s) 928
Fsp4HI GCNGC 8 cut(s) 69, 477, 563, 750, 843, 939, 1239, 1413
FspBI CTAG 2 cut(s) 437, 965
GlaI GCGC 1 cut(s) 792
GluI GCNGC 8 cut(s) 69, 477, 563, 750, 843, 939, 1239, 1413
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 4 cut(s) 201, 476, 685, 1011
HapII CCGG 9 cut(s) 270, 686, 902, 921, 1005, 1013, 1140, 1321, 1430
HgaI GACGC 3 cut(s) 314, 320, 797
HhaI GCGC 1 cut(s) 793
Hin1II CATG 9 cut(s) 314, 406, 643, 741, 838, 865, 986, 1026, 1295
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 8 cut(s) 156, 341, 524, 531, 571, 700, 783, 1107
HpaII CCGG 9 cut(s) 270, 686, 902, 921, 1005, 1013, 1140, 1321, 1430
HphI GGTGA 3 cut(s) 34, 416, 1513
Hpy166II GTNNAC 9 cut(s) 79, 121, 241, 691, 699, 735, 787, 887, 1389
Hpy188I TCNGA 4 cut(s) 133, 484, 678, 1106
Hpy188III TCNNGA 9 cut(s) 270, 403, 491, 528, 588, 951, 1052, 1083, 1577
Hpy8I GTNNAC 9 cut(s) 79, 121, 241, 691, 699, 735, 787, 887, 1389
Hpy99I CGWCG 6 cut(s) 218, 362, 446, 791, 980, 1163
HpyAV CCTTC 4 cut(s) 33, 480, 1027, 1223
HpyCH4IV ACGT 6 cut(s) 228, 596, 768, 825, 975, 1278
HpyCH4V TGCA 2 cut(s) 314, 774
HpyF10VI GCNNNNNNNGC 4 cut(s) 667, 758, 1034, 1421
HpyF3I CTNAG 7 cut(s) 58, 345, 1038, 1506, 1545, 1625, 1638
HpySE526I ACGT 6 cut(s) 228, 596, 768, 825, 975, 1278
Hsp92II CATG 9 cut(s) 314, 406, 643, 741, 838, 865, 986, 1026, 1295
HspAI GCGC 1 cut(s) 791
KflI GGGWCCC 1 cut(s) 1129
Kpn2I TCCGGA 1 cut(s) 269
Ksp22I TGATCA 1 cut(s) 1335
KspI CCGCGG 1 cut(s) 1416
Kzo9I GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
LmnI GCTCC 5 cut(s) 272, 758, 923, 931, 1582
Lsp1109I GCAGC 3 cut(s) 574, 950, 1250
LweI GCATC 2 cut(s) 488, 829
MaeI CTAG 2 cut(s) 437, 965
MaeII ACGT 6 cut(s) 228, 596, 768, 825, 975, 1278
MaeIII GTNAC 6 cut(s) 332, 826, 1211, 1301, 1501, 1539
MalI GATC 7 cut(s) 363, 542, 812, 1047, 1056, 1181, 1337
MboI GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
MboII GAAGA 5 cut(s) 143, 168, 1156, 1307, 1613
MflI RGATCY 1 cut(s) 540
MhlI GDGCHC 2 cut(s) 746, 928
MluCI AATT 3 cut(s) 367, 633, 1569
MlyI GAGTC 2 cut(s) 694, 792
MmeI TCCRAC 2 cut(s) 736, 1153
MroI TCCGGA 1 cut(s) 269
MseI TTAA 6 cut(s) 281, 995, 1187, 1314, 1404, 1651
MslI CAYNNNNRTG 3 cut(s) 720, 837, 1161
MspA1I CMGCKG 3 cut(s) 147, 1272, 1415
MspI CCGG 9 cut(s) 270, 686, 902, 921, 1005, 1013, 1140, 1321, 1430
MspR9I CCNGG 3 cut(s) 902, 1013, 1141
MvnI CGCG 3 cut(s) 791, 1112, 1415
MwoI GCNNNNNNNGC 4 cut(s) 667, 758, 1034, 1421
NciI CCSGG 3 cut(s) 902, 1013, 1141
NdeI CATATG 1 cut(s) 1340
NdeII GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
NlaIII CATG 9 cut(s) 314, 406, 643, 741, 838, 865, 986, 1026, 1295
NlaIV GGNNCC 8 cut(s) 223, 274, 542, 760, 1130, 1131, 1234, 1557
NmeAIII GCCGAG 4 cut(s) 30, 143, 165, 1393
NmuCI GTSAC 2 cut(s) 332, 1501
NspI RCATGY 3 cut(s) 314, 838, 865
NspV TTCGAA 2 cut(s) 26, 154
OliI CACNNNNGTG 2 cut(s) 837, 1161
PagI TCATGA 1 cut(s) 402
PciI ACATGT 1 cut(s) 834
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 357, 1167
PfeI GAWTC 6 cut(s) 156, 341, 524, 531, 571, 1107
PflMI CCANNNNNTGG 1 cut(s) 726
PinAI ACCGGT 1 cut(s) 1320
PkrI GCNGC 8 cut(s) 70, 478, 564, 751, 844, 940, 1240, 1414
PleI GAGTC 2 cut(s) 694, 791
PpsI GAGTC 2 cut(s) 694, 791
Ppu21I YACGTR 1 cut(s) 826
PpuMI RGGWCCY 2 cut(s) 221, 1129
PscI ACATGT 1 cut(s) 834
Psp124BI GAGCTC 1 cut(s) 928
Psp5II RGGWCCY 2 cut(s) 221, 1129
PspEI GGTNACC 1 cut(s) 1539
PspN4I GGNNCC 8 cut(s) 223, 274, 542, 760, 1130, 1131, 1234, 1557
PspPI GGNCC 4 cut(s) 129, 221, 1010, 1129
PspPPI RGGWCCY 2 cut(s) 221, 1129
PsuI RGATCY 1 cut(s) 540
RsaI GTAC 1 cut(s) 989
RsaNI GTAC 1 cut(s) 988
RseI CAYNNNNRTG 3 cut(s) 720, 837, 1161
SacI GAGCTC 1 cut(s) 928
SacII CCGCGG 1 cut(s) 1416
SalI GTCGAC 1 cut(s) 785
SaqAI TTAA 6 cut(s) 281, 995, 1187, 1314, 1404, 1651
SatI GCNGC 8 cut(s) 69, 477, 563, 750, 843, 939, 1239, 1413
Sau3AI GATC 7 cut(s) 361, 540, 810, 1045, 1054, 1179, 1335
Sau96I GGNCC 4 cut(s) 129, 221, 1010, 1129
SchI GAGTC 2 cut(s) 694, 792
ScrFI CCNGG 3 cut(s) 902, 1013, 1141
SduI GDGCHC 2 cut(s) 746, 928
SfaNI GCATC 2 cut(s) 488, 829
SfcI CTRYAG 1 cut(s) 1267
Sfr303I CCGCGG 1 cut(s) 1416
SfuI TTCGAA 2 cut(s) 26, 154
SgrBI CCGCGG 1 cut(s) 1416
SinI GGWCC 3 cut(s) 129, 221, 1129
SmiMI CAYNNNNRTG 3 cut(s) 720, 837, 1161
SmlI CTYRAG 1 cut(s) 949
SmoI CTYRAG 1 cut(s) 949
Sse9I AATT 3 cut(s) 367, 633, 1569
SspMI CTAG 2 cut(s) 437, 965
SstI GAGCTC 1 cut(s) 928
StyD4I CCNGG 3 cut(s) 900, 1011, 1139
StyI CCWWGG 3 cut(s) 502, 964, 1559
TaiI ACGT 6 cut(s) 231, 599, 771, 828, 978, 1281
TaqI TCGA 6 cut(s) 26, 154, 360, 387, 786, 813
TaqII GACCGA 2 cut(s) 78, 968
TasI AATT 3 cut(s) 367, 633, 1569
TauI GCSGC 5 cut(s) 71, 479, 752, 845, 1415
TfiI GAWTC 6 cut(s) 156, 341, 524, 531, 571, 1107
Tru1I TTAA 6 cut(s) 281, 995, 1187, 1314, 1404, 1651
Tru9I TTAA 6 cut(s) 281, 995, 1187, 1314, 1404, 1651
TscAI CASTG 2 cut(s) 343, 751
TseFI GTSAC 2 cut(s) 332, 1501
TseI GCWGC 3 cut(s) 562, 938, 1238
Tsp45I GTSAC 2 cut(s) 332, 1501
TspDTI ATGAA 3 cut(s) 391, 628, 1308
TspGWI ACGGA 8 cut(s) 81, 202, 226, 367, 533, 1013, 1159, 1272
TspRI CASTG 2 cut(s) 343, 751
Van91I CCANNNNNTGG 1 cut(s) 726
VpaK11BI GGWCC 3 cut(s) 129, 221, 1129
XceI RCATGY 3 cut(s) 314, 838, 865
XmaJI CCTAGG 1 cut(s) 964
XmiI GTMKAC 5 cut(s) 78, 240, 734, 786, 1388
XspI CTAG 2 cut(s) 437, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.