Rh3DG317800

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000677
Physical Location & Seq
Reverse (-)
34271803 .. 34272273
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG317800.1

Sequence Viewer

Length: 471 bp
ATGGTGGCCATCACCCACGTCGACGTGGTGGATCCGAATACGGGTGCGCCGGTTAAACGCGACGGTTTATCGCTCGGGGAGGTGGTTTTCAGAGGCGGGTCTGTGATGTTGGGTTACCTGAAAGACCCAAAAGGCACGGCTAGTTGCATGAAAGATGGTTTGTTTTATACCGGCGACGTGGCAGTGATGCACCCGGACGGGTATCTGGAGATCAAGGATCGATCTAAGGACGTGATCATTAGCGGGGGAGAGAACGTGAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCGGCGGTGAACGAGGCGGCAGTGGTGGCTAGGCCTGACGAGTTTTGGGGGGAGATGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTGGCGCAGAGGCCGACGGAGAAGGAGATAATAGAATATTGCAGGGGGGAAGTTGCCGCATTACATGGTGCCGAAGACGGTGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

156

Amino Acids

16.58

Weight (kDa)

4.6

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 90 - 148 3.4e-15 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 21
AccII CGCG 3 cut(s) 60, 296, 359
AciI CCGC 6 cut(s) 96, 243, 296, 299, 311, 438
AclWI GGATC 3 cut(s) 26, 39, 225
AcoI YGGCCR 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 41
AjiI CACGTC 5 cut(s) 19, 25, 178, 232, 382
AluBI AGCT 1 cut(s) 377
AluI AGCT 1 cut(s) 377
AlwI GGATC 3 cut(s) 26, 39, 225
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 3 cut(s) 6, 326, 392
ApeKI GCWGC 1 cut(s) 261
AspLEI GCGC 3 cut(s) 49, 359, 388
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 4, 313
AvaI CYCGRG 1 cut(s) 74
BalI TGGCCA 1 cut(s) 8
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 273
BccI CCATC 2 cut(s) 17, 149
BceAI ACGGC 2 cut(s) 153, 337
BclI TGATCA 1 cut(s) 234
BcnI CCSGG 1 cut(s) 194
BfaI CTAG 2 cut(s) 141, 324
BisI GCNGC 4 cut(s) 262, 297, 312, 438
BlsI GCNGC 4 cut(s) 263, 298, 313, 439
Bme1390I CCNGG 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 74
BmgBI CACGTC 5 cut(s) 19, 25, 178, 232, 382
BmiI GGNNCC 2 cut(s) 33, 451
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 177, 339
BpiI GAAGAC 1 cut(s) 462
BpmI CTGGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 220
BsaXI ACNNNNNCTCC 6 cut(s) 240, 260, 270, 290, 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse118I RCCGGY 2 cut(s) 49, 170
BseCI ATCGAT 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 273
Bsh1236I CGCG 3 cut(s) 60, 296, 359
BshFI GGCC 3 cut(s) 8, 328, 394
BshNI GGYRCC 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 50, 171, 194
BslI CCNNNNNNNGG 1 cut(s) 41
BsnI GGCC 3 cut(s) 8, 328, 394
BsoBI CYCGRG 1 cut(s) 74
Bsp143I GATC 5 cut(s) 31, 210, 217, 221, 234
BspACI CCGC 6 cut(s) 96, 243, 296, 299, 311, 438
BspANI GGCC 3 cut(s) 8, 328, 394
BspDI ATCGAT 1 cut(s) 220
BspFNI CGCG 3 cut(s) 60, 296, 359
BspLI GGNNCC 2 cut(s) 33, 451
BspPI GGATC 3 cut(s) 26, 39, 225
BspT107I GGYRCC 1 cut(s) 449
BsrFI RCCGGY 2 cut(s) 49, 170
BssAI RCCGGY 2 cut(s) 49, 170
BssMI GATC 5 cut(s) 31, 210, 217, 221, 234
Bst4CI ACNGT 2 cut(s) 65, 461
BstDEI CTNAG 1 cut(s) 225
BstEII GGTNACC 1 cut(s) 113
BstFNI CGCG 3 cut(s) 60, 296, 359
BstHHI GCGC 3 cut(s) 49, 359, 388
BstKTI GATC 5 cut(s) 34, 213, 220, 224, 237
BstMBI GATC 5 cut(s) 31, 210, 217, 221, 234
BstMWI GCNNNNNNNGC 2 cut(s) 320, 383
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 192
BstUI CGCG 3 cut(s) 60, 296, 359
BstV1I GCAGC 1 cut(s) 273
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 3 cut(s) 8, 328, 394
BsuTUI ATCGAT 1 cut(s) 220
BtrI CACGTC 5 cut(s) 19, 25, 178, 232, 382
BtsI GCAGTG 2 cut(s) 189, 321
BtsIMutI CAGTG 2 cut(s) 189, 321
CfoI GCGC 3 cut(s) 49, 359, 388
Cfr10I RCCGGY 2 cut(s) 49, 170
ClaI ATCGAT 1 cut(s) 220
CviAII CATG 2 cut(s) 148, 446
CviJI RGCY 7 cut(s) 8, 140, 323, 328, 369, 377, 394
CviKI_1 RGCY 7 cut(s) 8, 140, 323, 328, 369, 377, 394
DdeI CTNAG 1 cut(s) 225
DpnI GATC 5 cut(s) 33, 212, 219, 223, 236
DpnII GATC 5 cut(s) 31, 210, 217, 221, 234
EaeI YGGCCR 1 cut(s) 6
Eco147I AGGCCT 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 74
Eco91I GGTNACC 1 cut(s) 113
EcoO65I GGTNACC 1 cut(s) 113
FaeI CATG 2 cut(s) 151, 449
FaiI YATR 3 cut(s) 149, 168, 447
FatI CATG 2 cut(s) 147, 445
FauI CCCGC 2 cut(s) 89, 236
FbaI TGATCA 1 cut(s) 234
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 4 cut(s) 262, 297, 312, 438
Fsp4HI GCNGC 4 cut(s) 262, 297, 312, 438
FspBI CTAG 2 cut(s) 141, 324
GlaI GCGC 3 cut(s) 48, 358, 387
GluI GCNGC 4 cut(s) 262, 297, 312, 438
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 3 cut(s) 8, 328, 394
HapII CCGG 3 cut(s) 50, 171, 194
HhaI GCGC 3 cut(s) 49, 359, 388
Hin1II CATG 2 cut(s) 151, 449
Hin6I GCGC 3 cut(s) 47, 357, 386
HinP1I GCGC 3 cut(s) 47, 357, 386
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 1 cut(s) 275
HpaII CCGG 3 cut(s) 50, 171, 194
HphI GGTGA 2 cut(s) 4, 313
Hpy166II GTNNAC 2 cut(s) 22, 304
Hpy188I TCNGA 2 cut(s) 36, 92
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 2 cut(s) 22, 304
Hpy99I CGWCG 5 cut(s) 23, 26, 65, 179, 400
HpyAV CCTTC 1 cut(s) 397
HpyCH4III ACNGT 2 cut(s) 65, 461
HpyCH4IV ACGT 6 cut(s) 18, 24, 177, 231, 255, 381
HpyCH4V TGCA 3 cut(s) 147, 190, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 383
HpyF3I CTNAG 1 cut(s) 225
HpySE526I ACGT 6 cut(s) 18, 24, 177, 231, 255, 381
Hsp92II CATG 2 cut(s) 151, 449
HspAI GCGC 3 cut(s) 47, 357, 386
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 5 cut(s) 31, 210, 217, 221, 234
LpnPI CCDG 8 cut(s) 63, 131, 184, 191, 207, 342, 383, 409
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 2 cut(s) 177, 339
MaeI CTAG 2 cut(s) 141, 324
MaeII ACGT 6 cut(s) 18, 24, 177, 231, 255, 381
MaeIII GTNAC 1 cut(s) 113
MalI GATC 5 cut(s) 33, 212, 219, 223, 236
MboI GATC 5 cut(s) 31, 210, 217, 221, 234
MboII GAAGA 1 cut(s) 467
MflI RGATCY 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 8
MlyI GAGTC 1 cut(s) 284
MnlI CCTC 5 cut(s) 73, 86, 262, 301, 384
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 2 cut(s) 54, 469
Msp20I TGGCCA 1 cut(s) 8
MspI CCGG 3 cut(s) 50, 171, 194
MspR9I CCNGG 1 cut(s) 194
MvnI CGCG 3 cut(s) 60, 296, 359
MwoI GCNNNNNNNGC 2 cut(s) 320, 383
NciI CCSGG 1 cut(s) 194
NdeII GATC 5 cut(s) 31, 210, 217, 221, 234
NlaIII CATG 2 cut(s) 151, 449
NlaIV GGNNCC 2 cut(s) 33, 451
PceI AGGCCT 1 cut(s) 328
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PkrI GCNGC 4 cut(s) 263, 298, 313, 439
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PspEI GGTNACC 1 cut(s) 113
PspN4I GGNNCC 2 cut(s) 33, 451
PsuI RGATCY 1 cut(s) 31
SalI GTCGAC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 54, 469
SatI GCNGC 4 cut(s) 262, 297, 312, 438
Sau3AI GATC 5 cut(s) 31, 210, 217, 221, 234
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 194
SfaNI GCATC 2 cut(s) 177, 339
SgrDI CGTCGACG 1 cut(s) 20
SseBI AGGCCT 1 cut(s) 328
SsiI CCGC 6 cut(s) 96, 243, 296, 299, 311, 438
SspI AATATT 1 cut(s) 419
SspMI CTAG 2 cut(s) 141, 324
StuI AGGCCT 1 cut(s) 328
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 65, 461
TaiI ACGT 6 cut(s) 21, 27, 180, 234, 258, 384
TaqI TCGA 3 cut(s) 21, 220, 273
TauI GCSGC 3 cut(s) 299, 314, 440
Tru1I TTAA 2 cut(s) 54, 469
Tru9I TTAA 2 cut(s) 54, 469
TscAI CASTG 2 cut(s) 189, 321
TseI GCWGC 1 cut(s) 261
TspDTI ATGAA 1 cut(s) 164
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 2 cut(s) 189, 321
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 2 cut(s) 141, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.