MD09G1283000.v1.1

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000677
Physical Location & Seq
Forward (+)
36042568 .. 36044226
1659 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1283000.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGAAGACCTGAAGCCCACTTCAGTCAACTCATCTCCTCTAACCCCCTTGGGATTTCTGGAAAGAGCAGCCACCGTTTACGGCGACTGCCCCTCCGTTGTCTACGAAGACACCACCTACACGTGGACGCAGACCCACCGTCGCTGTCTCCGCGTCGCTTCCTCCATATCATCCCTCGGCATCGACAGAGGCCACGTGGTGTCTGTCCTTTCCCCAAACGTCCCCGCCATGTACGAGCTACACTTCGCCGTGCCCATGTCCGGCGCCATCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTACTCCTGCGCCACAGCGAATCAAAACTAGTCTTCGTCGACTACGAATTGCAATCCCTAGTCCTTGAAGCCATCTCTCTCTTCCCGCCAAATACCCCCTCCCCTAGACTCGTCCTCATCGCCAACGATTCCGGCGCGCCGTCACGCGCAGCACAACTCGACCACTTCCTCTGCACGTACGAGAACCTCGTGGAGACAGGCGACGAGGGGTTCGAGTGGGTCCCGCCGTCGAACGAGTGGGACCCCATAACGCTGAATTACACGTCGGGTACCACATCGTCTCCCAAGGGAGTAGTTCACTGCCACAGAGGGGCTTTCATCATCACCGTCGATTCCCTGATTGACTGGGGCGTACCCAAACAGCCCGTGTACCTGTGGACCCTACCAATGTTCCACTCTAACGGGTGGAACTTCCCATACGGGATGGCAGCCGTCGGTGGGACGAACATCTGTGTCCGTAAGTTCGACGGCCAAATCATCTACGCCATGATCAAACGACACGGTGTCACTCACATGTGCGGTGCGCCCGTGGTGCTGAACATGCTCACAAACGTCCGGTCCGGAACCGAAAAGCTCGAGTACCCAGTACACATCCTAACCGGCGGAGCACCGCCGCCGGCTCAGGTTCTCATCCGGACCGAGTCCATGGGTTTCGTGGTGAGTCACGGGTACGGGCTGACCGAAACGGCGGGGGTGGTGGTGTCGTGCGCGTGGAAGGGGAAGTGGAATTTGTTGCCGGCGACGGAGAGAGCGAGGCTGAAGTCGAGGCAAGGAGTGAGGACGGTCGGAATGACCCGAGTCGACGTGGTTGACCCGGACTCGGGACTCAGCGTAAAACGCGACGGAATGTCGCTCGGCGAGATCGTTCTGAGAGGCGGGTGCGTGATGCTCGGGTATCTGAAGGACCCATCGGGAACGGCCAAGTGCATGAAAGGCGGGTGGTTTTATACTGGTGACGTGGGGGTAATTCATCCCGATGGCTATTTGGAAATCAAGGACAGATCCAAGGACGTTATTATAAGCGGGGGCGAGAACCTGAGCAGCGTGGAGGTGGAGTCAGTGCTGTATACGAACCCGGCTGTCGACGAGGCGGCGGTTGTGGCGAGGCCTGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTTGTGAGCTTGAAGAGTGATGTGACGGCGAAGCCAACGGAGAATGAGATCATGGAGTATTGCAGGAAGAAGCTGCCGAAATACATGGTGCCAAAAACGGTGGTGTTTAAGGACGAGCTTCCCAAGACTTCGACCGGGAAGATTCAGAAGTTTGTGCTCAGACAGATGGCTAAGGGTTTGGGTTCGTCGAGGCTGAGTCGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

553

Amino Acids

60.48

Weight (kDa)

7.18

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 404 1.9e-73 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 3.5e-26 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1331
Acc65I GGTACC 1 cut(s) 581
AccB1I GGYRCC 3 cut(s) 263, 581, 1540
AccI GTMKAC 5 cut(s) 102, 351, 1113, 1379, 1395
AccII CGCG 6 cut(s) 153, 449, 459, 1022, 1152, 1451
AccIII TCCGGA 2 cut(s) 872, 945
AclWI GGATC 1 cut(s) 1308
AcoI YGGCCR 2 cut(s) 781, 1230
AcsI RAATTY 1 cut(s) 1039
AcuI CTGAAG 4 cut(s) 6, 32, 1091, 1232
AcvI CACGTG 2 cut(s) 123, 196
AcyI GRCGYC 2 cut(s) 264, 1442
AdeI CACNNNGTG 1 cut(s) 199
AfaI GTAC 8 cut(s) 233, 491, 583, 666, 683, 893, 900, 983
AfiI CCNNNNNNNGG 6 cut(s) 123, 260, 622, 750, 1132, 1133
AflIII ACRYGT 3 cut(s) 120, 573, 825
AgsI TTSAA 2 cut(s) 380, 1465
AhdI GACNNNNNGTC 3 cut(s) 138, 815, 1159
AhlI ACTAGT 1 cut(s) 340
AjiI CACGTC 3 cut(s) 576, 1117, 1270
AluBI AGCT 5 cut(s) 238, 886, 1461, 1525, 1570
AluI AGCT 5 cut(s) 238, 886, 1461, 1525, 1570
Alw21I GWGCWC 2 cut(s) 922, 1611
Alw26I GTCTC 5 cut(s) 152, 313, 500, 597, 1433
AlwI GGATC 1 cut(s) 1308
Ama87I CYCGRG 4 cut(s) 887, 1107, 1132, 1202
Aor13HI TCCGGA 2 cut(s) 872, 945
AoxI GGCC 4 cut(s) 190, 781, 1230, 1418
ApeKI GCWGC 5 cut(s) 68, 461, 740, 1353, 1525
ApoI RAATTY 1 cut(s) 1039
ArsI GACNNNNNNTTYG 4 cut(s) 506, 538, 854, 886
AscI GGCGCGCC 1 cut(s) 447
Asp718I GGTACC 1 cut(s) 581
AspLEI GCGC 8 cut(s) 266, 324, 449, 451, 461, 838, 1022, 1451
AspS9I GGNCC 6 cut(s) 532, 553, 690, 870, 948, 1216
AsuC2I CCSGG 3 cut(s) 1127, 1388, 1588
AsuHPI GGTGA 3 cut(s) 628, 982, 1277
AvaI CYCGRG 4 cut(s) 887, 1107, 1132, 1202
AvaII GGWCC 6 cut(s) 532, 553, 690, 870, 948, 1216
BaeGI GKGCMC 1 cut(s) 255
BanI GGYRCC 3 cut(s) 263, 581, 1540
BauI CACGAG 1 cut(s) 500
BbrPI CACGTG 2 cut(s) 123, 196
BbsI GAAGAC 3 cut(s) 12, 114, 337
Bbv12I GWGCWC 2 cut(s) 922, 1611
BbvI GCAGC 5 cut(s) 80, 473, 752, 1365, 1512
BccI CCATC 7 cut(s) 275, 287, 392, 730, 1228, 1283, 1612
BclI TGATCA 1 cut(s) 801
BcnI CCSGG 3 cut(s) 1127, 1388, 1588
BcoDI GTCTC 5 cut(s) 152, 313, 500, 597, 1433
BcuI ACTAGT 1 cut(s) 340
BfaI CTAG 3 cut(s) 341, 371, 417
BfoI RGCGCY 1 cut(s) 267
BisI GCNGC 7 cut(s) 69, 462, 741, 926, 1354, 1404, 1526
BlsI GCNGC 7 cut(s) 70, 463, 742, 927, 1355, 1405, 1527
Bme1390I CCNGG 3 cut(s) 1127, 1388, 1588
Bme18I GGWCC 6 cut(s) 532, 553, 690, 870, 948, 1216
BmeRI GACNNNNNGTC 3 cut(s) 138, 815, 1159
BmeT110I CYCGRG 4 cut(s) 887, 1107, 1132, 1202
BmgBI CACGTC 3 cut(s) 576, 1117, 1270
BmgT120I GGNCC 6 cut(s) 532, 553, 690, 870, 948, 1216
BmrFI CCNGG 3 cut(s) 1127, 1388, 1588
BmrI ACTGGG 2 cut(s) 667, 890
BmsI GCATC 2 cut(s) 189, 1188
BmuI ACTGGG 2 cut(s) 667, 890
BoxI GACNNNNGTC 1 cut(s) 1109
BpiI GAAGAC 3 cut(s) 12, 114, 337
Bpu10I CCTNAGC 3 cut(s) 933, 1349, 1623
BpuMI CCSGG 3 cut(s) 1127, 1388, 1588
BsaAI YACGTR 3 cut(s) 123, 196, 489
BsaHI GRCGYC 2 cut(s) 264, 1442
BsaJI CCNNGG 6 cut(s) 48, 175, 597, 840, 957, 1317
BsaWI WCCGGW 3 cut(s) 867, 872, 945
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 577, 607, 1352, 1382, 1430, 1460
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 260, 622, 750, 1132, 1133
Bse118I RCCGGY 3 cut(s) 911, 928, 1048
Bse1I ACTGG 3 cut(s) 662, 896, 1267
BseAI TCCGGA 2 cut(s) 872, 945
BseDI CCNNGG 6 cut(s) 48, 175, 597, 840, 957, 1317
BseGI GGATG 7 cut(s) 170, 267, 741, 903, 942, 1282, 1659
BseLI CCNNNNNNNGG 6 cut(s) 123, 260, 622, 750, 1132, 1133
BseMII CTCAG 6 cut(s) 947, 1153, 1172, 1340, 1624, 1637
BseNI ACTGG 3 cut(s) 662, 896, 1267
BsePI GCGCGC 1 cut(s) 447
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 255
BseXI GCAGC 5 cut(s) 80, 473, 752, 1365, 1512
BsgI GTGCAG 1 cut(s) 469
Bsh1236I CGCG 6 cut(s) 153, 449, 459, 1022, 1152, 1451
Bsh1285I CGRYCG 2 cut(s) 1098, 1587
BshFI GGCC 4 cut(s) 192, 783, 1232, 1420
BshNI GGYRCC 3 cut(s) 263, 581, 1540
BsiEI CGRYCG 2 cut(s) 1098, 1587
BsiHKAI GWGCWC 2 cut(s) 922, 1611
BsiHKCI CYCGRG 4 cut(s) 887, 1107, 1132, 1202
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 5 cut(s) 206, 518, 566, 766, 1149
BslI CCNNNNNNNGG 6 cut(s) 123, 260, 622, 750, 1132, 1133
BsmAI GTCTC 5 cut(s) 152, 313, 500, 597, 1433
BsmBI CGTCTC 3 cut(s) 313, 597, 1433
BsmFI GGGAC 5 cut(s) 206, 518, 566, 766, 1149
BsnI GGCC 4 cut(s) 192, 783, 1232, 1420
BsoBI CYCGRG 4 cut(s) 887, 1107, 1132, 1202
Bsp1286I GDGCHC 3 cut(s) 255, 922, 1611
Bsp13I TCCGGA 2 cut(s) 872, 945
Bsp143I GATC 4 cut(s) 801, 1173, 1313, 1500
Bsp19I CCATGG 1 cut(s) 957
BspANI GGCC 4 cut(s) 192, 783, 1232, 1420
BspCNI CTCAG 6 cut(s) 946, 1152, 1173, 1341, 1623, 1638
BspEI TCCGGA 2 cut(s) 872, 945
BspFNI CGCG 6 cut(s) 153, 449, 459, 1022, 1152, 1451
BspPI GGATC 1 cut(s) 1308
BspT107I GGYRCC 3 cut(s) 263, 581, 1540
BsrFI RCCGGY 3 cut(s) 911, 928, 1048
BsrI ACTGG 3 cut(s) 662, 896, 1267
BssAI RCCGGY 3 cut(s) 911, 928, 1048
BssECI CCNNGG 6 cut(s) 48, 175, 597, 840, 957, 1317
BssHII GCGCGC 1 cut(s) 447
BssMI GATC 4 cut(s) 801, 1173, 1313, 1500
BssNAI GTATAC 1 cut(s) 1380
BssNI GRCGYC 2 cut(s) 264, 1442
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 4 cut(s) 48, 597, 957, 1317
Bst1107I GTATAC 1 cut(s) 1380
Bst2BI CACGAG 1 cut(s) 500
Bst4CI ACNGT 7 cut(s) 76, 140, 307, 640, 815, 1096, 1552
Bst6I CTCTTC 2 cut(s) 398, 1460
BstACI GRCGYC 2 cut(s) 264, 1442
BstBAI YACGTR 3 cut(s) 123, 196, 489
BstC8I GCNNGC 3 cut(s) 449, 930, 1050
BstDEI CTNAG 7 cut(s) 933, 1139, 1181, 1349, 1610, 1623, 1646
BstDSI CCRYGG 2 cut(s) 840, 957
BstF5I GGATG 7 cut(s) 170, 267, 741, 903, 942, 1282, 1659
BstFNI CGCG 6 cut(s) 153, 449, 459, 1022, 1152, 1451
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 8 cut(s) 266, 324, 449, 451, 461, 838, 1022, 1451
BstKTI GATC 4 cut(s) 804, 1176, 1316, 1503
BstMAI GTCTC 5 cut(s) 152, 313, 500, 597, 1433
BstMBI GATC 4 cut(s) 801, 1173, 1313, 1500
BstMCI CGRYCG 2 cut(s) 1098, 1587
BstMWI GCNNNNNNNGC 6 cut(s) 150, 844, 853, 1149, 1245, 1412
BstNSI RCATGY 2 cut(s) 829, 856
BstPAI GACNNNNGTC 1 cut(s) 1109
BstSCI CCNGG 3 cut(s) 1125, 1386, 1586
BstSLI GKGCMC 1 cut(s) 255
BstUI CGCG 6 cut(s) 153, 449, 459, 1022, 1152, 1451
BstV1I GCAGC 5 cut(s) 80, 473, 752, 1365, 1512
BstV2I GAAGAC 3 cut(s) 12, 114, 337
BstX2I RGATCY 1 cut(s) 1313
BstYI RGATCY 1 cut(s) 1313
BstZ17I GTATAC 1 cut(s) 1380
BsuRI GGCC 4 cut(s) 192, 783, 1232, 1420
BtgI CCRYGG 2 cut(s) 840, 957
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 3 cut(s) 576, 1117, 1270
BtsCI GGATG 7 cut(s) 170, 267, 741, 903, 942, 1282, 1659
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 2 cut(s) 610, 1377
Cac8I GCNNGC 3 cut(s) 449, 930, 1050
CfoI GCGC 8 cut(s) 266, 324, 449, 451, 461, 838, 1022, 1451
Cfr10I RCCGGY 3 cut(s) 911, 928, 1048
Cfr13I GGNCC 6 cut(s) 532, 553, 690, 870, 948, 1216
CpoI CGGWCCG 2 cut(s) 870, 948
CseI GACGC 3 cut(s) 136, 142, 1450
Csp6I GTAC 8 cut(s) 232, 490, 582, 665, 682, 892, 899, 982
CspCI CAANNNNNGTGG 2 cut(s) 1533, 1568
CspI CGGWCCG 2 cut(s) 870, 948
CviAII CATG 9 cut(s) 229, 256, 799, 826, 853, 958, 1240, 1504, 1537
CviQI GTAC 8 cut(s) 232, 490, 582, 665, 682, 892, 899, 982
DdeI CTNAG 7 cut(s) 933, 1139, 1181, 1349, 1610, 1623, 1646
DinI GGCGCC 1 cut(s) 265
DpnI GATC 4 cut(s) 803, 1175, 1315, 1502
DpnII GATC 4 cut(s) 801, 1173, 1313, 1500
DraIII CACNNNGTG 1 cut(s) 199
DriI GACNNNNNGTC 3 cut(s) 138, 815, 1159
EaeI YGGCCR 2 cut(s) 781, 1230
Eam1104I CTCTTC 2 cut(s) 398, 1460
Eam1105I GACNNNNNGTC 3 cut(s) 138, 815, 1159
EarI CTCTTC 2 cut(s) 398, 1460
EciI GGCGGA 1 cut(s) 930
Eco130I CCWWGG 4 cut(s) 48, 597, 957, 1317
Eco147I AGGCCT 1 cut(s) 1420
Eco47I GGWCC 6 cut(s) 532, 553, 690, 870, 948, 1216
Eco57I CTGAAG 4 cut(s) 6, 32, 1091, 1232
Eco72I CACGTG 2 cut(s) 123, 196
Eco88I CYCGRG 4 cut(s) 887, 1107, 1132, 1202
EcoO109I RGGNCCY 3 cut(s) 532, 553, 1216
EcoT14I CCWWGG 4 cut(s) 48, 597, 957, 1317
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 4 cut(s) 48, 597, 957, 1317
Esp3I CGTCTC 3 cut(s) 313, 597, 1433
FaeI CATG 9 cut(s) 232, 259, 802, 829, 856, 961, 1243, 1507, 1540
FaqI GGGAC 5 cut(s) 206, 518, 566, 766, 1149
FatI CATG 9 cut(s) 228, 255, 798, 825, 852, 957, 1239, 1503, 1536
FauI CCCGC 9 cut(s) 232, 296, 308, 405, 543, 994, 1181, 1241, 1328
FbaI TGATCA 1 cut(s) 801
FblI GTMKAC 5 cut(s) 102, 351, 1113, 1379, 1395
Fnu4HI GCNGC 7 cut(s) 69, 462, 741, 926, 1354, 1404, 1526
FokI GGATG 6 cut(s) 157, 254, 748, 890, 929, 1269
Fsp4HI GCNGC 7 cut(s) 69, 462, 741, 926, 1354, 1404, 1526
FspBI CTAG 3 cut(s) 341, 371, 417
GlaI GCGC 8 cut(s) 265, 323, 448, 450, 460, 837, 1021, 1450
GluI GCNGC 7 cut(s) 69, 462, 741, 926, 1354, 1404, 1526
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 4 cut(s) 192, 783, 1232, 1420
HgaI GACGC 3 cut(s) 136, 142, 1450
HhaI GCGC 8 cut(s) 266, 324, 449, 451, 461, 838, 1022, 1451
Hin1I GRCGYC 2 cut(s) 264, 1442
Hin1II CATG 9 cut(s) 232, 259, 802, 829, 856, 961, 1243, 1507, 1540
Hin6I GCGC 8 cut(s) 264, 322, 447, 449, 459, 836, 1020, 1449
HinP1I GCGC 8 cut(s) 264, 322, 447, 449, 459, 836, 1020, 1449
HincII GTYRAC 5 cut(s) 28, 352, 1114, 1123, 1396
HindII GTYRAC 5 cut(s) 28, 352, 1114, 1123, 1396
HphI GGTGA 3 cut(s) 628, 982, 1277
Hpy188I TCNGA 6 cut(s) 1100, 1182, 1212, 1599, 1613, 1653
Hpy188III TCNNGA 6 cut(s) 59, 873, 946, 1134, 1224, 1286
HpyAV CCTTC 2 cut(s) 1021, 1207
HpyCH4III ACNGT 7 cut(s) 76, 140, 307, 640, 815, 1096, 1552
HpyCH4IV ACGT 9 cut(s) 122, 195, 219, 488, 575, 864, 1116, 1269, 1323
HpyCH4V TGCA 4 cut(s) 364, 486, 1239, 1515
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 844, 853, 1149, 1245, 1412
HpyF3I CTNAG 7 cut(s) 933, 1139, 1181, 1349, 1610, 1623, 1646
HpySE526I ACGT 9 cut(s) 122, 195, 219, 488, 575, 864, 1116, 1269, 1323
Hsp92I GRCGYC 2 cut(s) 264, 1442
Hsp92II CATG 9 cut(s) 232, 259, 802, 829, 856, 961, 1243, 1507, 1540
HspAI GCGC 8 cut(s) 264, 322, 447, 449, 459, 836, 1020, 1449
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 2 cut(s) 532, 553
Kpn2I TCCGGA 2 cut(s) 872, 945
KpnI GGTACC 1 cut(s) 585
KroI GCCGGC 2 cut(s) 928, 1048
KroNI GCCGGC 2 cut(s) 930, 1050
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 4 cut(s) 801, 1173, 1313, 1500
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 5 cut(s) 80, 473, 752, 1365, 1512
LweI GCATC 2 cut(s) 189, 1188
MaeI CTAG 3 cut(s) 341, 371, 417
MaeII ACGT 9 cut(s) 122, 195, 219, 488, 575, 864, 1116, 1269, 1323
MaeIII GTNAC 5 cut(s) 453, 817, 974, 1265, 1474
MalI GATC 4 cut(s) 803, 1175, 1315, 1502
MboI GATC 4 cut(s) 801, 1173, 1313, 1500
MboII GAAGA 7 cut(s) 17, 119, 337, 385, 1477, 1531, 1603
MflI RGATCY 1 cut(s) 1313
MhlI GDGCHC 3 cut(s) 255, 922, 1611
MluCI AATT 4 cut(s) 359, 568, 1039, 1278
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 8 cut(s) 414, 962, 982, 1119, 1124, 1131, 1376, 1657
MmeI TCCRAC 2 cut(s) 1078, 1631
MroI TCCGGA 2 cut(s) 872, 945
MroNI GCCGGC 2 cut(s) 928, 1048
MseI TTAA 1 cut(s) 1560
MslI CAYNNNNRTG 2 cut(s) 824, 1287
MspR9I CCNGG 3 cut(s) 1127, 1388, 1588
MvnI CGCG 6 cut(s) 153, 449, 459, 1022, 1152, 1451
MwoI GCNNNNNNNGC 6 cut(s) 150, 844, 853, 1149, 1245, 1412
NaeI GCCGGC 2 cut(s) 930, 1050
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 3 cut(s) 1127, 1388, 1588
NcoI CCATGG 1 cut(s) 957
NdeII GATC 4 cut(s) 801, 1173, 1313, 1500
NgoMIV GCCGGC 2 cut(s) 928, 1048
NlaIII CATG 9 cut(s) 232, 259, 802, 829, 856, 961, 1243, 1507, 1540
NmeAIII GCCGAG 2 cut(s) 156, 1146
NmuCI GTSAC 5 cut(s) 453, 817, 974, 1265, 1474
NspI RCATGY 2 cut(s) 829, 856
PaeR7I CTCGAG 1 cut(s) 887
PalAI GGCGCGCC 1 cut(s) 447
PauI GCGCGC 1 cut(s) 447
PceI AGGCCT 1 cut(s) 1420
PciI ACATGT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 7 cut(s) 354, 498, 522, 990, 1061, 1173, 1448
PdiI GCCGGC 2 cut(s) 930, 1050
PfeI GAWTC 4 cut(s) 332, 440, 644, 1594
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 1 cut(s) 952
PkrI GCNGC 7 cut(s) 70, 463, 742, 927, 1355, 1405, 1527
PleI GAGTC 8 cut(s) 414, 961, 981, 1118, 1124, 1131, 1375, 1656
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 2 cut(s) 123, 196
PmlI CACGTG 2 cut(s) 123, 196
PpsI GAGTC 8 cut(s) 414, 961, 981, 1118, 1124, 1131, 1375, 1656
Ppu21I YACGTR 3 cut(s) 123, 196, 489
PpuMI RGGWCCY 3 cut(s) 532, 553, 1216
PscI ACATGT 1 cut(s) 825
PshAI GACNNNNGTC 1 cut(s) 1109
PsiI TTATAA 1 cut(s) 1331
Psp5II RGGWCCY 3 cut(s) 532, 553, 1216
PspCI CACGTG 2 cut(s) 123, 196
PspLI CGTACG 1 cut(s) 489
PspPI GGNCC 6 cut(s) 532, 553, 690, 870, 948, 1216
PspPPI RGGWCCY 3 cut(s) 532, 553, 1216
PspXI VCTCGAGB 1 cut(s) 887
PsrI GAACNNNNNNTAC 2 cut(s) 713, 745
PsuI RGATCY 1 cut(s) 1313
PsyI GACNNNGTC 1 cut(s) 952
PteI GCGCGC 1 cut(s) 447
RsaI GTAC 8 cut(s) 233, 491, 583, 666, 683, 893, 900, 983
RsaNI GTAC 8 cut(s) 232, 490, 582, 665, 682, 892, 899, 982
RseI CAYNNNNRTG 2 cut(s) 824, 1287
Rsr2I CGGWCCG 2 cut(s) 870, 948
RsrII CGGWCCG 2 cut(s) 870, 948
SalI GTCGAC 3 cut(s) 350, 1112, 1394
SaqAI TTAA 1 cut(s) 1560
SatI GCNGC 7 cut(s) 69, 462, 741, 926, 1354, 1404, 1526
Sau3AI GATC 4 cut(s) 801, 1173, 1313, 1500
Sau96I GGNCC 6 cut(s) 532, 553, 690, 870, 948, 1216
SchI GAGTC 8 cut(s) 414, 962, 982, 1119, 1124, 1131, 1376, 1657
ScrFI CCNGG 3 cut(s) 1127, 1388, 1588
SduI GDGCHC 3 cut(s) 255, 922, 1611
SfaNI GCATC 2 cut(s) 189, 1188
SfoI GGCGCC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 887
SgsI GGCGCGCC 1 cut(s) 447
SinI GGWCC 6 cut(s) 532, 553, 690, 870, 948, 1216
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 2 cut(s) 824, 1287
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
SpeI ACTAGT 1 cut(s) 340
Sse9I AATT 4 cut(s) 359, 568, 1039, 1278
SseBI AGGCCT 1 cut(s) 1420
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 3 cut(s) 341, 371, 417
StuI AGGCCT 1 cut(s) 1420
StyD4I CCNGG 3 cut(s) 1125, 1386, 1586
StyI CCWWGG 4 cut(s) 48, 597, 957, 1317
TaaI ACNGT 7 cut(s) 76, 140, 307, 640, 815, 1096, 1552
TaiI ACGT 9 cut(s) 125, 198, 222, 491, 578, 867, 1119, 1272, 1326
TaqII GACCGA 2 cut(s) 965, 1007
TasI AATT 4 cut(s) 359, 568, 1039, 1278
TatI WGTACW 1 cut(s) 898
TauI GCSGC 2 cut(s) 928, 1406
TfiI GAWTC 4 cut(s) 332, 440, 644, 1594
Tru1I TTAA 1 cut(s) 1560
Tru9I TTAA 1 cut(s) 1560
TscAI CASTG 2 cut(s) 617, 1377
TseFI GTSAC 5 cut(s) 453, 817, 974, 1265, 1474
TseI GCWGC 5 cut(s) 68, 461, 740, 1353, 1525
Tsp45I GTSAC 5 cut(s) 453, 817, 974, 1265, 1474
TspDTI ATGAA 3 cut(s) 619, 1256, 1271
TspGWI ACGGA 5 cut(s) 85, 758, 1070, 1170, 1505
TspRI CASTG 2 cut(s) 617, 1377
Tth111I GACNNNGTC 1 cut(s) 952
VpaK11BI GGWCC 6 cut(s) 532, 553, 690, 870, 948, 1216
XapI RAATTY 1 cut(s) 1039
XceI RCATGY 2 cut(s) 829, 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 964
XhoI CTCGAG 1 cut(s) 887
XmiI GTMKAC 5 cut(s) 102, 351, 1113, 1379, 1395
XspI CTAG 3 cut(s) 341, 371, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.