FvH4_1g27170

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000677
Physical Location & Seq
Reverse (-)
18928723 .. 18930393
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27170.t1

Sequence Viewer

Length: 1671 bp
ATGGACCAGTTGAAACCTAGTACTGCAAATCTCTGCCGTCAAACCCCCCTTGACTTCTTGGAACGAGCAGCTATCGTCTACGGCGACTGCCCCTCCATCATCTACAGCTCCACCACCACCTTCACCTGGTCCCAAACCCACCGTCGATGTCTCCAGCTGGCCTCCTCCATCTCCACCGTCGTCGACAAGTCCCAGGTTGTCTCCGTGGTGGCCCCCAACACTCCTGCCATGTACGAACTCCAGTTCGCTGTTCCCATGTCGGGAGCCATCCTCAACAACATCAACACCCGCTTCGACCCCCACGCCGTCTCCGTGATCCTCCAACACAGCCAATCCAAGCTCGTCTTCGTCGACTCCCTCTCCCTCACCCTCGTCCTCCACGCCCTCTCCTTGTTCCCCTCTCACATCCCAACTCCGCCTCTCGTCCTCATCAACGATCTTCACTCCACTTCCAGCTCAGTCTCCGCCGTCGACCTCGTAGTCGACACGTACGACAACATGGTGGCGAAAGGCGACCCCGGATTCGTTTGGGTCCGGCCGGCAAGCGAGTGGGACCCGATGGTGCTCAACTACACGTCAGGGACGACGTCGGCTCCGAAAGGCGTAGTGCACTGCCACAGGGCTCTGTTCCTCGTCAGCCTAGTCTCTATCATCGACTGGTCGGTACCCAAGAACTGTGTGTACCTGTGGACCCTACCGATGTTCCATGCCAACGGGTGGTGCTTCCCGTGGGCCATGGCCGCCGTGGGTGGGACCAACATCTGCCTCCGCAAGTTCGACGGTCCTGTAATCTACGATCTCATTCAGAAACACAGAGTCACTCACATGTGCGCTGCACCTGTCGTTCTCAACATGCTGGTCAACCACCCGAGACCCGACCCGCTTCCGAACCCGGTTTGCATCTTGACCGGCGGAGCTCCGCCTCCTTCGAGCGTGCTGCTCCGGGCGGAGTCGCTAGGGTTTGTTGTCAGTCACGGCTACGGGTTGACTGAAACGGCCGGTGTGGTGGTGTCGTGTGCGTGGAAGCCGGAGTGGGGTAAGTTTCCGGTGACGGAGAAGGCGAGGTTGAAGGCCAGGCAAGGAGTGGGGACGGTTGGGACGACGGAGGTGGATGTGGTGGATCCGGATTCGGGTTTGAGCGTGAAGCGAAACGGGTCTGATATTGGAGAGATCGTTATTCGAGGCGGGTGCGTCATGTTGGGCTATTTGAAAGACCCGGAAACGACGAGCAGAGCCCTGAAACGTAACGGGTGGTTTTACACCGGCGATGTCGGGGTTATGCATCCGGACGGGTACCTGGAGATAAAGGACCGATCCAAGGACGTGATCATAAGCGGTGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTGTACACCCACGCCGCCGTGAACGAGGCGGCTGTGGTGGCCAGGCCTGACGAGTTTTGGGGGGAGACGCCTTGCGCGTTTGTGAGCCTGAAGGGTGACGTAAGCCCGAGGCCGACGGAGAAGGAGATAATAGAGTATTGTAGAGGGAAGCTGCCGAGTTTCATGGTCCCGAAGACGGTGGTGTTCAAGGAGGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTCTGTGCTGAGAGAGATTGCTAAAAGTATTGGGTCATCGTCGGCGTCGACGATGATTAGTCGATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

557

Amino Acids

60.45

Weight (kDa)

6.94

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 403 9.5e-73 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 5.4e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 479
AatII GACGTC 2 cut(s) 590, 1583
Acc65I GGTACC 2 cut(s) 664, 1293
AccB1I GGYRCC 2 cut(s) 664, 1293
AccB7I CCANNNNNTGG 1 cut(s) 717
AccI GTMKAC 7 cut(s) 78, 183, 351, 471, 483, 1583, 1649
AccII CGCG 1 cut(s) 1451
AccIII TCCGGA 2 cut(s) 1123, 1285
AclWI GGATC 4 cut(s) 310, 1115, 1128, 1308
AcoI YGGCCR 4 cut(s) 536, 738, 996, 1413
AcuI CTGAAG 1 cut(s) 1484
AcyI GRCGYC 4 cut(s) 587, 1442, 1580, 1646
AfaI GTAC 7 cut(s) 22, 233, 491, 666, 683, 1295, 1379
AfiI CCNNNNNNNGG 8 cut(s) 126, 260, 717, 750, 1034, 1130, 1318, 1549
AflIII ACRYGT 3 cut(s) 486, 573, 825
AgsI TTSAA 4 cut(s) 13, 1069, 1210, 1561
AjiI CACGTC 2 cut(s) 576, 1324
AjnI CCWGG 5 cut(s) 125, 192, 1073, 1296, 1415
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 8 cut(s) 71, 108, 157, 340, 456, 917, 1525, 1570
AluI AGCT 8 cut(s) 71, 108, 157, 340, 456, 917, 1525, 1570
Alw21I GWGCWC 3 cut(s) 567, 612, 919
Alw26I GTCTC 7 cut(s) 155, 205, 313, 466, 649, 865, 1433
Alw44I GTGCAC 1 cut(s) 608
AlwI GGATC 4 cut(s) 310, 1115, 1128, 1308
Ama87I CYCGRG 2 cut(s) 868, 1480
Aor13HI TCCGGA 2 cut(s) 1123, 1285
ApaLI GTGCAC 1 cut(s) 608
ApeKI GCWGC 6 cut(s) 68, 833, 937, 1353, 1525, 1570
ArsI GACNNNNNNTTYG 4 cut(s) 127, 159, 506, 538
Asp718I GGTACC 2 cut(s) 664, 1293
AspLEI GCGC 2 cut(s) 833, 1451
AsuC2I CCSGG 4 cut(s) 519, 893, 944, 1217
AsuHPI GGTGA 4 cut(s) 115, 358, 1060, 1481
AvaI CYCGRG 2 cut(s) 868, 1480
AvaII GGWCC 9 cut(s) 4, 129, 532, 553, 690, 753, 782, 1309, 1540
BaeGI GKGCMC 1 cut(s) 612
BalI TGGCCA 1 cut(s) 1415
BamHI GGATCC 1 cut(s) 1120
BanI GGYRCC 2 cut(s) 664, 1293
BanII GRGCYC 3 cut(s) 625, 919, 1237
BbsI GAAGAC 3 cut(s) 337, 1553, 1583
Bbv12I GWGCWC 3 cut(s) 567, 612, 919
BbvI GCAGC 6 cut(s) 80, 820, 924, 1365, 1512, 1557
BccI CCATC 4 cut(s) 104, 176, 275, 553
BceAI ACGGC 8 cut(s) 21, 97, 290, 452, 728, 991, 1011, 1376
BcgI CGANNNNNNTGC 4 cut(s) 919, 953, 1170, 1204
BciT130I CCWGG 5 cut(s) 127, 194, 1075, 1298, 1417
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 4 cut(s) 519, 893, 944, 1217
BcoDI GTCTC 7 cut(s) 155, 205, 313, 466, 649, 865, 1433
BfaI CTAG 3 cut(s) 18, 641, 956
BfmI CTRYAG 1 cut(s) 103
BisI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
BlsI GCNGC 9 cut(s) 70, 742, 835, 939, 1355, 1390, 1405, 1527, 1572
BmcAI AGTACT 1 cut(s) 22
Bme1390I CCNGG 9 cut(s) 127, 194, 519, 893, 944, 1075, 1217, 1298, 1417
Bme18I GGWCC 9 cut(s) 4, 129, 532, 553, 690, 753, 782, 1309, 1540
BmeT110I CYCGRG 2 cut(s) 868, 1480
BmgBI CACGTC 2 cut(s) 576, 1324
BmrFI CCNGG 9 cut(s) 127, 194, 519, 893, 944, 1075, 1217, 1298, 1417
BmsI GCATC 2 cut(s) 909, 1291
BpiI GAAGAC 3 cut(s) 337, 1553, 1583
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 3 cut(s) 137, 224, 1319
BpuEI CTTGAG 1 cut(s) 1369
BpuMI CCSGG 4 cut(s) 519, 893, 944, 1217
BsaAI YACGTR 1 cut(s) 489
BsaHI GRCGYC 4 cut(s) 587, 1442, 1580, 1646
BsaI GGTCTC 1 cut(s) 865
BsaJI CCNNGG 8 cut(s) 192, 204, 517, 728, 735, 744, 1317, 1481
BsaWI WCCGGW 3 cut(s) 1045, 1123, 1285
Bsc4I CCNNNNNNNGG 8 cut(s) 126, 260, 717, 750, 1034, 1130, 1318, 1549
Bse118I RCCGGY 4 cut(s) 538, 908, 998, 1262
Bse1I ACTGG 3 cut(s) 7, 241, 662
BseAI TCCGGA 2 cut(s) 1123, 1285
BseBI CCWGG 5 cut(s) 127, 194, 1075, 1298, 1417
BseDI CCNNGG 8 cut(s) 192, 204, 517, 728, 735, 744, 1317, 1481
BseGI GGATG 4 cut(s) 267, 405, 1117, 1282
BseLI CCNNNNNNNGG 8 cut(s) 126, 260, 717, 750, 1034, 1130, 1318, 1549
BseMII CTCAG 2 cut(s) 471, 1601
BseNI ACTGG 3 cut(s) 7, 241, 662
BseRI GAGGAG 2 cut(s) 154, 1580
BseSI GKGCMC 1 cut(s) 612
BseX3I CGGCCG 2 cut(s) 536, 996
BseXI GCAGC 6 cut(s) 80, 820, 924, 1365, 1512, 1557
BsgI GTGCAG 1 cut(s) 819
Bsh1236I CGCG 1 cut(s) 1451
Bsh1285I CGRYCG 2 cut(s) 539, 999
BshNI GGYRCC 2 cut(s) 664, 1293
BsiEI CGRYCG 2 cut(s) 539, 999
BsiHKAI GWGCWC 3 cut(s) 567, 612, 919
BsiHKCI CYCGRG 2 cut(s) 868, 1480
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 8 cut(s) 115, 175, 566, 595, 766, 1102, 1111, 1526
BslI CCNNNNNNNGG 8 cut(s) 126, 260, 717, 750, 1034, 1130, 1318, 1549
BsmAI GTCTC 7 cut(s) 155, 205, 313, 466, 649, 865, 1433
BsmBI CGTCTC 2 cut(s) 313, 1433
BsmFI GGGAC 8 cut(s) 115, 175, 566, 595, 766, 1102, 1111, 1526
Bso31I GGTCTC 1 cut(s) 865
BsoBI CYCGRG 2 cut(s) 868, 1480
Bsp1286I GDGCHC 5 cut(s) 567, 612, 625, 919, 1237
Bsp13I TCCGGA 2 cut(s) 1123, 1285
Bsp1407I TGTACA 1 cut(s) 1377
Bsp143I GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
Bsp19I CCATGG 1 cut(s) 735
BspCNI CTCAG 2 cut(s) 470, 1602
BspEI TCCGGA 2 cut(s) 1123, 1285
BspFNI CGCG 1 cut(s) 1451
BspPI GGATC 4 cut(s) 310, 1115, 1128, 1308
BspT107I GGYRCC 2 cut(s) 664, 1293
BspTNI GGTCTC 1 cut(s) 865
BsrFI RCCGGY 4 cut(s) 538, 908, 998, 1262
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 3 cut(s) 7, 241, 662
BssAI RCCGGY 4 cut(s) 538, 908, 998, 1262
BssECI CCNNGG 8 cut(s) 192, 204, 517, 728, 735, 744, 1317, 1481
BssMI GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
BssNI GRCGYC 4 cut(s) 587, 1442, 1580, 1646
BssT1I CCWWGG 2 cut(s) 735, 1317
Bst2UI CCWGG 5 cut(s) 127, 194, 1075, 1298, 1417
Bst4CI ACNGT 6 cut(s) 143, 178, 677, 782, 1093, 1552
BstACI GRCGYC 4 cut(s) 587, 1442, 1580, 1646
BstAUI TGTACA 1 cut(s) 1377
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 159, 540, 544, 935
BstDEI CTNAG 2 cut(s) 457, 1610
BstDSI CCRYGG 4 cut(s) 204, 728, 735, 744
BstF5I GGATG 4 cut(s) 267, 405, 1117, 1282
BstFNI CGCG 1 cut(s) 1451
BstHHI GCGC 2 cut(s) 833, 1451
BstKTI GATC 7 cut(s) 318, 439, 799, 1123, 1173, 1316, 1329
BstMAI GTCTC 7 cut(s) 155, 205, 313, 466, 649, 865, 1433
BstMBI GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
BstMCI CGRYCG 2 cut(s) 539, 999
BstMWI GCNNNNNNNGC 2 cut(s) 740, 1412
BstNI CCWGG 5 cut(s) 127, 194, 1075, 1298, 1417
BstNSI RCATGY 2 cut(s) 829, 856
BstSCI CCNGG 9 cut(s) 125, 192, 517, 891, 942, 1073, 1215, 1296, 1415
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 612
BstUI CGCG 1 cut(s) 1451
BstV1I GCAGC 6 cut(s) 80, 820, 924, 1365, 1512, 1557
BstV2I GAAGAC 3 cut(s) 337, 1553, 1583
BstX2I RGATCY 1 cut(s) 1120
BstYI RGATCY 1 cut(s) 1120
BstZI CGGCCG 2 cut(s) 536, 996
BtgI CCRYGG 4 cut(s) 204, 728, 735, 744
BtgZI GCGATG 1 cut(s) 1281
BtrI CACGTC 2 cut(s) 576, 1324
BtsCI GGATG 4 cut(s) 267, 405, 1117, 1282
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 610
Cac8I GCNNGC 4 cut(s) 159, 540, 544, 935
CfoI GCGC 2 cut(s) 833, 1451
Cfr10I RCCGGY 4 cut(s) 538, 908, 998, 1262
CseI GACGC 3 cut(s) 1180, 1450, 1635
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 7 cut(s) 21, 232, 490, 665, 682, 1294, 1378
CviAII CATG 9 cut(s) 229, 256, 499, 707, 736, 826, 853, 1195, 1537
CviQI GTAC 7 cut(s) 21, 232, 490, 665, 682, 1294, 1378
DdeI CTNAG 2 cut(s) 457, 1610
DpnI GATC 7 cut(s) 317, 438, 798, 1122, 1172, 1315, 1328
DpnII GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
DrdI GACNNNNNNGTC 1 cut(s) 479
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 4 cut(s) 536, 738, 996, 1413
EagI CGGCCG 2 cut(s) 536, 996
EciI GGCGGA 5 cut(s) 405, 454, 909, 927, 962
Ecl136II GAGCTC 1 cut(s) 917
EclXI CGGCCG 2 cut(s) 536, 996
Eco130I CCWWGG 2 cut(s) 735, 1317
Eco147I AGGCCT 1 cut(s) 1420
Eco24I GRGCYC 3 cut(s) 625, 919, 1237
Eco31I GGTCTC 1 cut(s) 865
Eco47I GGWCC 9 cut(s) 4, 129, 532, 553, 690, 753, 782, 1309, 1540
Eco52I CGGCCG 2 cut(s) 536, 996
Eco53kI GAGCTC 1 cut(s) 917
Eco57I CTGAAG 1 cut(s) 1484
Eco88I CYCGRG 2 cut(s) 868, 1480
EcoICRI GAGCTC 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 5 cut(s) 125, 192, 1073, 1296, 1415
EcoT14I CCWWGG 2 cut(s) 735, 1317
EcoT22I ATGCAT 1 cut(s) 1284
EcoT38I GRGCYC 3 cut(s) 625, 919, 1237
ErhI CCWWGG 2 cut(s) 735, 1317
Esp3I CGTCTC 2 cut(s) 313, 1433
FaeI CATG 9 cut(s) 232, 259, 502, 710, 739, 829, 856, 1198, 1540
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 8 cut(s) 115, 175, 566, 595, 766, 1102, 1111, 1526
FatI CATG 9 cut(s) 228, 255, 498, 706, 735, 825, 852, 1194, 1536
FauI CCCGC 3 cut(s) 296, 888, 1178
FbaI TGATCA 1 cut(s) 1326
FblI GTMKAC 7 cut(s) 78, 183, 351, 471, 483, 1583, 1649
Fnu4HI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
FokI GGATG 4 cut(s) 254, 392, 1124, 1269
FriOI GRGCYC 3 cut(s) 625, 919, 1237
Fsp4HI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
FspBI CTAG 3 cut(s) 18, 641, 956
GlaI GCGC 2 cut(s) 832, 1450
GluI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
GsuI CTGGAG 3 cut(s) 137, 224, 1319
HgaI GACGC 3 cut(s) 1180, 1450, 1635
HhaI GCGC 2 cut(s) 833, 1451
Hin1I GRCGYC 4 cut(s) 587, 1442, 1580, 1646
Hin1II CATG 9 cut(s) 232, 259, 502, 710, 739, 829, 856, 1198, 1540
Hin6I GCGC 2 cut(s) 831, 1449
HinP1I GCGC 2 cut(s) 831, 1449
HincII GTYRAC 8 cut(s) 184, 352, 472, 484, 862, 987, 1584, 1650
HindII GTYRAC 8 cut(s) 184, 352, 472, 484, 862, 987, 1584, 1650
HinfI GANTC 7 cut(s) 353, 522, 816, 950, 1127, 1367, 1594
HphI GGTGA 4 cut(s) 115, 358, 1060, 1481
Hpy188I TCNGA 5 cut(s) 597, 807, 888, 1159, 1599
Hpy188III TCNNGA 5 cut(s) 261, 904, 1124, 1286, 1543
HpyAV CCTTC 6 cut(s) 130, 936, 1051, 1063, 1459, 1489
HpyCH4III ACNGT 6 cut(s) 143, 178, 677, 782, 1093, 1552
HpyCH4IV ACGT 7 cut(s) 488, 575, 587, 1243, 1323, 1473, 1580
HpyCH4V TGCA 5 cut(s) 26, 610, 836, 900, 1282
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 1412
HpyF3I CTNAG 2 cut(s) 457, 1610
HpySE526I ACGT 7 cut(s) 488, 575, 587, 1243, 1323, 1473, 1580
Hsp92I GRCGYC 4 cut(s) 587, 1442, 1580, 1646
Hsp92II CATG 9 cut(s) 232, 259, 502, 710, 739, 829, 856, 1198, 1540
HspAI GCGC 2 cut(s) 831, 1449
KflI GGGWCCC 1 cut(s) 553
Kpn2I TCCGGA 2 cut(s) 1123, 1285
KpnI GGTACC 2 cut(s) 668, 1297
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
LmnI GCTCC 7 cut(s) 113, 263, 598, 914, 922, 945, 1567
Lsp1109I GCAGC 6 cut(s) 80, 820, 924, 1365, 1512, 1557
LweI GCATC 2 cut(s) 909, 1291
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 3 cut(s) 18, 641, 956
MaeII ACGT 7 cut(s) 488, 575, 587, 1243, 1323, 1473, 1580
MaeIII GTNAC 5 cut(s) 817, 971, 1048, 1244, 1469
MalI GATC 7 cut(s) 317, 438, 798, 1122, 1172, 1315, 1328
MboI GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
MboII GAAGA 5 cut(s) 337, 431, 1558, 1588, 1603
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 5 cut(s) 567, 612, 625, 919, 1237
MlsI TGGCCA 1 cut(s) 1415
MluNI TGGCCA 1 cut(s) 1415
MlyI GAGTC 4 cut(s) 347, 825, 959, 1376
MmeI TCCRAC 1 cut(s) 346
Mox20I TGGCCA 1 cut(s) 1415
Mph1103I ATGCAT 1 cut(s) 1284
MroI TCCGGA 2 cut(s) 1123, 1285
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 1415
MslI CAYNNNNRTG 1 cut(s) 824
Msp20I TGGCCA 1 cut(s) 1415
MspA1I CMGCKG 1 cut(s) 157
MspR9I CCNGG 9 cut(s) 127, 194, 519, 893, 944, 1075, 1217, 1298, 1417
MvaI CCWGG 5 cut(s) 127, 194, 1075, 1298, 1417
MvnI CGCG 1 cut(s) 1451
MwoI GCNNNNNNNGC 2 cut(s) 740, 1412
NaeI GCCGGC 1 cut(s) 540
NciI CCSGG 4 cut(s) 519, 893, 944, 1217
NcoI CCATGG 1 cut(s) 735
NdeII GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 9 cut(s) 232, 259, 502, 710, 739, 829, 856, 1198, 1540
NmeAIII GCCGAG 1 cut(s) 1554
NmuCI GTSAC 4 cut(s) 817, 971, 1048, 1469
NsiI ATGCAT 1 cut(s) 1284
NspI RCATGY 2 cut(s) 829, 856
PceI AGGCCT 1 cut(s) 1420
PciI ACATGT 1 cut(s) 825
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 522, 1127, 1594
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 1 cut(s) 586
PflMI CCANNNNNTGG 1 cut(s) 717
PkrI GCNGC 9 cut(s) 70, 742, 835, 939, 1355, 1390, 1405, 1527, 1572
PleI GAGTC 4 cut(s) 347, 824, 958, 1375
PpsI GAGTC 4 cut(s) 347, 824, 958, 1375
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 553
PscI ACATGT 1 cut(s) 825
Psp124BI GAGCTC 1 cut(s) 919
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 5 cut(s) 125, 192, 1073, 1296, 1415
PspGI CCWGG 5 cut(s) 125, 192, 1073, 1296, 1415
PspLI CGTACG 1 cut(s) 489
PspPPI RGGWCCY 1 cut(s) 553
PsrI GAACNNNNNNTAC 2 cut(s) 665, 697
PsuI RGATCY 1 cut(s) 1120
PsyI GACNNNGTC 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 7 cut(s) 22, 233, 491, 666, 683, 1295, 1379
RsaNI GTAC 7 cut(s) 21, 232, 490, 665, 682, 1294, 1378
RseI CAYNNNNRTG 1 cut(s) 824
SacI GAGCTC 1 cut(s) 919
SalI GTCGAC 6 cut(s) 182, 350, 470, 482, 1582, 1648
SatI GCNGC 9 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1526, 1571
Sau3AI GATC 7 cut(s) 315, 436, 796, 1120, 1170, 1313, 1326
ScaI AGTACT 1 cut(s) 22
SchI GAGTC 4 cut(s) 347, 825, 959, 1376
ScrFI CCNGG 9 cut(s) 127, 194, 519, 893, 944, 1075, 1217, 1298, 1417
SduI GDGCHC 5 cut(s) 567, 612, 625, 919, 1237
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 2 cut(s) 909, 1291
SfcI CTRYAG 1 cut(s) 103
SgrAI CRCCGGYG 1 cut(s) 1262
SgrDI CGTCGACG 2 cut(s) 1582, 1648
SinI GGWCC 9 cut(s) 4, 129, 532, 553, 690, 753, 782, 1309, 1540
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 1 cut(s) 1348
SmoI CTYRAG 1 cut(s) 1348
SseBI AGGCCT 1 cut(s) 1420
SspMI CTAG 3 cut(s) 18, 641, 956
SstI GAGCTC 1 cut(s) 919
StuI AGGCCT 1 cut(s) 1420
StyD4I CCNGG 9 cut(s) 125, 192, 517, 891, 942, 1073, 1215, 1296, 1415
StyI CCWWGG 2 cut(s) 735, 1317
TaaI ACNGT 6 cut(s) 143, 178, 677, 782, 1093, 1552
TaiI ACGT 7 cut(s) 491, 578, 590, 1246, 1326, 1476, 1583
TaqII GACCGA 1 cut(s) 1326
TatI WGTACW 2 cut(s) 20, 1377
TauI GCSGC 3 cut(s) 743, 1391, 1406
TfiI GAWTC 3 cut(s) 522, 1127, 1594
TscAI CASTG 1 cut(s) 617
TseFI GTSAC 4 cut(s) 817, 971, 1048, 1469
TseI GCWGC 6 cut(s) 68, 833, 937, 1353, 1525, 1570
Tsp45I GTSAC 4 cut(s) 817, 971, 1048, 1469
TspDTI ATGAA 1 cut(s) 1525
TspGWI ACGGA 5 cut(s) 193, 301, 1067, 1118, 1505
TspRI CASTG 1 cut(s) 617
Tth111I GACNNNGTC 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 717
VneI GTGCAC 1 cut(s) 608
VpaK11BI GGWCC 9 cut(s) 4, 129, 532, 553, 690, 753, 782, 1309, 1540
XceI RCATGY 2 cut(s) 829, 856
XcmI CCANNNNNNNNNTGG 2 cut(s) 742, 1081
XmiI GTMKAC 7 cut(s) 78, 183, 351, 471, 483, 1583, 1649
XspI CTAG 3 cut(s) 18, 641, 956
ZraI GACGTC 2 cut(s) 588, 1581
ZrmI AGTACT 1 cut(s) 22
Zsp2I ATGCAT 1 cut(s) 1284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.