FvH4_1g27180

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000677
Physical Location & Seq
Reverse (-)
18937887 .. 18939920
2034 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27180.t1

Sequence Viewer

Length: 1644 bp
ATGGAGGAGCTGAAGCCAAGCCCTTGCAACTCATCCCCTCTCACCCCACTGGGTTTCCTCGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCCCTCATCTACGACGACATCACCTACACGTGGAGCCAGACCCACCGCCGATGCCTCCGCCTCGCTTCCTCCATCTCCTCCCTCCTCGGCCCCCAACGCGGCCACGTGGTCTCCGTCCTCTCCCCCAACACCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCTTCTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCCTCGACCACTCCCACACCTCCCTCGCCCTCCACGCCATCTCTCTCTTCCCTCCGGACACCCCCGCCCCTCTCCTCGTCCTCATCACCGACGATGCTCCCGCGCCCACCTCCCCCTTCTGCGCCACCTACGAGGATATGATCGAGAGCGGCGACCCGGAGTTCCGGTGGGACCTACCTGACAGCGAGTGGGACCCGATGACGCTCAACTACACTTCCGGGACGACTTCCTCGCCCAAAGGAGTGGTCCACTGCCACCGGGGGATGTTCATCGTCACAGTCAACTCTCTCCTTGACTGGGCCGTGCCCAAGCAGCCCGTCTATCTCTGGACGCTTCCCATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTGGGCGGGACCAACGTCTGCGTCCGCAAGTTCGACGCGCGTGTCATCTACGACCTGATCACACGACACGGCGTCACCCACATGTGCGGCGCGCCGGTGGTCCTCAACATGCTGACCAACATACCCAACGTCGAGCCCTTGAGGAGCCCCGTGCATATCTTGACCGCCGGGGCTCCGCCTCCCGCGGCGGTTCTCGGTCGGACCGAGTCGCTGGGGTTCGTGGTGAGCCATGGGTACGGGCTGACGGAGACGGCGGGGCTGGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAACAAGTTTCCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGACGGTGGCCATGACCCGGGTCGACGTGGTAGATCCGGATACGGGTAAGCCGGTTAAACGCGACGGCGCGTCGCTTGGGGAAGTGGTTTTGAGAGGCGGGTGTGTGATGTTAGGTTACCTGAAAGACCCAAAAGGGACGGCGAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATAAAGGACCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTGTACACCCACGCCGCCGTGAACGAGGCGGCGGTGGTGGCCAGGCCTGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTTGTGAGCTTGAAGGGTGACGTGAGCCCGAGGCCGACGGAGAAGGAGATAATGGAGTATTGTAGAGGGAAGCTGCCGAGTTTCATGGCGCCGAAGACGGTGGTGTTCAAGGAGGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTTGTGCTGAGAGAGATGGCCAAGGCGTTGGGGTCCACAAGGCTGAGTCGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

548

Amino Acids

59.82

Weight (kDa)

6.96

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 399 3.7e-74 AMP-binding enzyme
AMP-binding_C PF13193 449 - 526 9.6e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 773
AatII GACGTC 1 cut(s) 1568
AccB1I GGYRCC 2 cut(s) 266, 1525
AccB7I CCANNNNNTGG 1 cut(s) 705
AccBSI CCGCTC 1 cut(s) 498
AccI GTMKAC 3 cut(s) 78, 1098, 1568
AccIII TCCGGA 2 cut(s) 403, 1111
AclWI GGATC 3 cut(s) 731, 1103, 1293
AcoI YGGCCR 4 cut(s) 193, 1083, 1398, 1605
AcuI CTGAAG 2 cut(s) 32, 1076
AcvI CACGTG 2 cut(s) 123, 199
AcyI GRCGYC 5 cut(s) 267, 804, 1427, 1526, 1565
AfaI GTAC 4 cut(s) 236, 719, 968, 1364
AfiI CCNNNNNNNGG 9 cut(s) 123, 191, 263, 646, 705, 738, 1092, 1118, 1303
AflIII ACRYGT 2 cut(s) 120, 813
AgsI TTSAA 2 cut(s) 1450, 1546
AhdI GACNNNNNGTC 2 cut(s) 803, 1144
AjiI CACGTC 4 cut(s) 1102, 1255, 1309, 1459
AjnI CCWGG 1 cut(s) 1400
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 7 cut(s) 10, 241, 343, 711, 1446, 1510, 1555
AluI AGCT 7 cut(s) 10, 241, 343, 711, 1446, 1510, 1555
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 4 cut(s) 208, 316, 974, 1418
AlwI GGATC 3 cut(s) 731, 1103, 1293
Ama87I CYCGRG 3 cut(s) 1016, 1092, 1465
Aor13HI TCCGGA 2 cut(s) 403, 1111
AoxI GGCC 8 cut(s) 181, 193, 648, 1083, 1398, 1403, 1469, 1605
ApeKI GCWGC 4 cut(s) 661, 1338, 1510, 1555
ArsI GACNNNNNNTTYG 2 cut(s) 579, 611
AscI GGCGCGCC 1 cut(s) 822
AsuC2I CCSGG 9 cut(s) 506, 568, 608, 901, 1017, 1018, 1093, 1094, 1271
AsuHPI GGTGA 7 cut(s) 34, 106, 427, 799, 967, 1273, 1466
AvaI CYCGRG 3 cut(s) 1016, 1092, 1465
AvaII GGWCC 8 cut(s) 520, 541, 595, 741, 832, 933, 1294, 1620
BaeGI GKGCMC 1 cut(s) 657
BalI TGGCCA 3 cut(s) 1085, 1400, 1607
BanI GGYRCC 2 cut(s) 266, 1525
BanII GRGCYC 5 cut(s) 243, 870, 881, 907, 1466
BbrPI CACGTG 2 cut(s) 123, 199
BbsI GAAGAC 2 cut(s) 1538, 1568
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 673, 1350, 1497, 1542
BccI CCATC 4 cut(s) 173, 290, 395, 1597
BciT130I CCWGG 1 cut(s) 1402
BciVI GTATCC 1 cut(s) 1108
BclI TGATCA 2 cut(s) 789, 1311
BcnI CCSGG 9 cut(s) 506, 568, 608, 901, 1017, 1018, 1093, 1094, 1271
BcoDI GTCTC 4 cut(s) 208, 316, 974, 1418
BfoI RGCGCY 2 cut(s) 270, 1529
BfuI GTATCC 1 cut(s) 1108
Bme18I GGWCC 8 cut(s) 520, 541, 595, 741, 832, 933, 1294, 1620
BmeRI GACNNNNNGTC 2 cut(s) 803, 1144
BmeT110I CYCGRG 3 cut(s) 1016, 1092, 1465
BmgBI CACGTC 4 cut(s) 1102, 1255, 1309, 1459
BmrI ACTGGG 2 cut(s) 59, 655
BmsI GCATC 3 cut(s) 134, 433, 1254
BmuI ACTGGG 2 cut(s) 59, 655
BoxI GACNNNNGTC 2 cut(s) 746, 1094
BpiI GAAGAC 2 cut(s) 1538, 1568
BpmI CTGGAG 2 cut(s) 727, 1304
BpuEI CTTGAG 2 cut(s) 892, 1354
BpuMI CCSGG 9 cut(s) 506, 568, 608, 901, 1017, 1018, 1093, 1094, 1271
BsaAI YACGTR 2 cut(s) 123, 199
BsaBI GATNNNNATC 1 cut(s) 617
BsaHI GRCGYC 5 cut(s) 267, 804, 1427, 1526, 1565
BsaI GGTCTC 1 cut(s) 208
BsaWI WCCGGW 3 cut(s) 403, 513, 1111
BsaXI ACNNNNNCTCC 8 cut(s) 188, 218, 500, 530, 1337, 1367, 1415, 1445
Bsc4I CCNNNNNNNGG 9 cut(s) 123, 191, 263, 646, 705, 738, 1092, 1118, 1303
Bse118I RCCGGY 3 cut(s) 826, 1126, 1247
Bse1I ACTGG 2 cut(s) 54, 650
Bse8I GATNNNNATC 1 cut(s) 617
BseAI TCCGGA 2 cut(s) 403, 1111
BseBI CCWGG 1 cut(s) 1402
BseGI GGATG 3 cut(s) 32, 618, 1644
BseJI GATNNNNATC 1 cut(s) 617
BseLI CCNNNNNNNGG 9 cut(s) 123, 191, 263, 646, 705, 738, 1092, 1118, 1303
BseMII CTCAG 2 cut(s) 1586, 1622
BseNI ACTGG 2 cut(s) 54, 650
BsePI GCGCGC 1 cut(s) 822
BseRI GAGGAG 9 cut(s) 20, 160, 167, 308, 311, 335, 413, 889, 1565
BseSI GKGCMC 1 cut(s) 657
BseXI GCAGC 4 cut(s) 673, 1350, 1497, 1542
BseYI CCCAGC 1 cut(s) 943
Bsh1285I CGRYCG 2 cut(s) 64, 931
BshFI GGCC 8 cut(s) 183, 195, 650, 1085, 1400, 1405, 1471, 1607
BshNI GGYRCC 2 cut(s) 266, 1525
BsiEI CGRYCG 2 cut(s) 64, 931
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 3 cut(s) 1016, 1092, 1465
BsiWI CGTACG 1 cut(s) 717
BslFI GGGAC 6 cut(s) 239, 533, 554, 583, 754, 1225
BslI CCNNNNNNNGG 9 cut(s) 123, 191, 263, 646, 705, 738, 1092, 1118, 1303
BsmAI GTCTC 4 cut(s) 208, 316, 974, 1418
BsmBI CGTCTC 3 cut(s) 316, 974, 1418
BsmFI GGGAC 6 cut(s) 239, 533, 554, 583, 754, 1225
BsnI GGCC 8 cut(s) 183, 195, 650, 1085, 1400, 1405, 1471, 1607
Bso31I GGTCTC 1 cut(s) 208
BsoBI CYCGRG 3 cut(s) 1016, 1092, 1465
Bsp1286I GDGCHC 6 cut(s) 243, 657, 870, 881, 907, 1466
Bsp13I TCCGGA 2 cut(s) 403, 1111
Bsp1407I TGTACA 1 cut(s) 1362
Bsp143I GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
Bsp19I CCATGG 1 cut(s) 961
BspANI GGCC 8 cut(s) 183, 195, 650, 1085, 1400, 1405, 1471, 1607
BspCNI CTCAG 2 cut(s) 1587, 1623
BspEI TCCGGA 2 cut(s) 403, 1111
BspPI GGATC 3 cut(s) 731, 1103, 1293
BspT107I GGYRCC 2 cut(s) 266, 1525
BspTNI GGTCTC 1 cut(s) 208
BsrBI CCGCTC 1 cut(s) 498
BsrFI RCCGGY 3 cut(s) 826, 1126, 1247
BsrGI TGTACA 1 cut(s) 1362
BsrI ACTGG 2 cut(s) 54, 650
BssAI RCCGGY 3 cut(s) 826, 1126, 1247
BssHII GCGCGC 1 cut(s) 822
BssMI GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
BssNI GRCGYC 5 cut(s) 267, 804, 1427, 1526, 1565
BssT1I CCWWGG 3 cut(s) 961, 1302, 1608
Bst2UI CCWGG 1 cut(s) 1402
Bst4CI ACNGT 6 cut(s) 76, 310, 628, 1081, 1235, 1537
Bst6I CTCTTC 2 cut(s) 353, 401
BstACI GRCGYC 5 cut(s) 267, 804, 1427, 1526, 1565
BstAUI TGTACA 1 cut(s) 1362
BstBAI YACGTR 2 cut(s) 123, 199
BstC8I GCNNGC 1 cut(s) 824
BstDEI CTNAG 2 cut(s) 1595, 1631
BstDSI CCRYGG 3 cut(s) 732, 915, 961
BstEII GGTNACC 1 cut(s) 1190
BstF5I GGATG 3 cut(s) 32, 618, 1644
BstH2I RGCGCY 2 cut(s) 270, 1529
BstKTI GATC 6 cut(s) 492, 726, 792, 1111, 1301, 1314
BstMAI GTCTC 4 cut(s) 208, 316, 974, 1418
BstMBI GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
BstMCI CGRYCG 2 cut(s) 64, 931
BstMWI GCNNNNNNNGC 4 cut(s) 189, 383, 661, 1397
BstNI CCWGG 1 cut(s) 1402
BstNSI RCATGY 2 cut(s) 817, 844
BstPAI GACNNNNGTC 2 cut(s) 746, 1094
BstPI GGTNACC 1 cut(s) 1190
BstSLI GKGCMC 1 cut(s) 657
BstV1I GCAGC 4 cut(s) 673, 1350, 1497, 1542
BstV2I GAAGAC 2 cut(s) 1538, 1568
BstX2I RGATCY 1 cut(s) 1108
BstXI CCANNNNNNTGG 2 cut(s) 592, 1615
BstYI RGATCY 1 cut(s) 1108
BsuI GTATCC 1 cut(s) 1108
BsuRI GGCC 8 cut(s) 183, 195, 650, 1085, 1400, 1405, 1471, 1607
BtgI CCRYGG 3 cut(s) 732, 915, 961
BtrI CACGTC 4 cut(s) 1102, 1255, 1309, 1459
BtsCI GGATG 3 cut(s) 32, 618, 1644
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 2 cut(s) 47, 598
Cac8I GCNNGC 1 cut(s) 824
Cfr10I RCCGGY 3 cut(s) 826, 1126, 1247
Cfr42I CCGCGG 1 cut(s) 918
Cfr9I CCCGGG 2 cut(s) 1016, 1092
CpoI CGGWCCG 1 cut(s) 933
CseI GACGC 7 cut(s) 559, 688, 742, 776, 793, 1134, 1435
Csp6I GTAC 4 cut(s) 235, 718, 967, 1363
CspI CGGWCCG 1 cut(s) 933
CviAII CATG 8 cut(s) 232, 688, 814, 841, 962, 1087, 1225, 1522
CviQI GTAC 4 cut(s) 235, 718, 967, 1363
DdeI CTNAG 2 cut(s) 1595, 1631
DinI GGCGCC 2 cut(s) 268, 1527
DpnI GATC 6 cut(s) 491, 725, 791, 1110, 1300, 1313
DpnII GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
DrdI GACNNNNNNGTC 1 cut(s) 773
DriI GACNNNNNGTC 2 cut(s) 803, 1144
DseDI GACNNNNNNGTC 1 cut(s) 773
EaeI YGGCCR 4 cut(s) 193, 1083, 1398, 1605
Eam1104I CTCTTC 2 cut(s) 353, 401
Eam1105I GACNNNNNGTC 2 cut(s) 803, 1144
EarI CTCTTC 2 cut(s) 353, 401
EciI GGCGGA 3 cut(s) 140, 314, 897
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 3 cut(s) 961, 1302, 1608
Eco147I AGGCCT 1 cut(s) 1405
Eco24I GRGCYC 5 cut(s) 243, 870, 881, 907, 1466
Eco31I GGTCTC 1 cut(s) 208
Eco47I GGWCC 8 cut(s) 520, 541, 595, 741, 832, 933, 1294, 1620
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 32, 1076
Eco72I CACGTG 2 cut(s) 123, 199
Eco88I CYCGRG 3 cut(s) 1016, 1092, 1465
Eco91I GGTNACC 1 cut(s) 1190
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 2 cut(s) 520, 541
EcoO65I GGTNACC 1 cut(s) 1190
EcoRII CCWGG 1 cut(s) 1400
EcoT14I CCWWGG 3 cut(s) 961, 1302, 1608
EcoT38I GRGCYC 5 cut(s) 243, 870, 881, 907, 1466
EgeI GGCGCC 2 cut(s) 268, 1527
EheI GGCGCC 2 cut(s) 268, 1527
ErhI CCWWGG 3 cut(s) 961, 1302, 1608
Esp3I CGTCTC 3 cut(s) 316, 974, 1418
FaeI CATG 8 cut(s) 235, 691, 817, 844, 965, 1090, 1228, 1525
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FaqI GGGAC 6 cut(s) 239, 533, 554, 583, 754, 1225
FatI CATG 8 cut(s) 231, 687, 813, 840, 961, 1086, 1224, 1521
FbaI TGATCA 2 cut(s) 789, 1311
FblI GTMKAC 3 cut(s) 78, 1098, 1568
FokI GGATG 2 cut(s) 19, 625
FriOI GRGCYC 5 cut(s) 243, 870, 881, 907, 1466
GsaI CCCAGC 1 cut(s) 947
GsuI CTGGAG 2 cut(s) 727, 1304
HaeII RGCGCY 2 cut(s) 270, 1529
HaeIII GGCC 8 cut(s) 183, 195, 650, 1085, 1400, 1405, 1471, 1607
HgaI GACGC 7 cut(s) 559, 688, 742, 776, 793, 1134, 1435
Hin1I GRCGYC 5 cut(s) 267, 804, 1427, 1526, 1565
Hin1II CATG 8 cut(s) 235, 691, 817, 844, 965, 1090, 1228, 1525
HincII GTYRAC 3 cut(s) 631, 1099, 1569
HindII GTYRAC 3 cut(s) 631, 1099, 1569
HinfI GANTC 4 cut(s) 938, 1352, 1579, 1633
HphI GGTGA 7 cut(s) 34, 106, 427, 799, 967, 1273, 1466
Hpy166II GTNNAC 8 cut(s) 79, 598, 631, 1099, 1365, 1381, 1569, 1623
Hpy188I TCNGA 3 cut(s) 933, 1584, 1638
Hpy188III TCNNGA 6 cut(s) 404, 493, 676, 892, 1112, 1283
Hpy8I GTNNAC 8 cut(s) 79, 598, 631, 1099, 1365, 1381, 1569, 1623
HpyAV CCTTC 5 cut(s) 102, 268, 475, 1444, 1474
HpyCH4III ACNGT 6 cut(s) 76, 310, 628, 1081, 1235, 1537
HpyCH4IV ACGT 9 cut(s) 122, 198, 747, 861, 1101, 1254, 1308, 1458, 1565
HpyCH4V TGCA 4 cut(s) 27, 886, 1224, 1267
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 383, 661, 1397
HpyF3I CTNAG 2 cut(s) 1595, 1631
HpySE526I ACGT 9 cut(s) 122, 198, 747, 861, 1101, 1254, 1308, 1458, 1565
Hsp92I GRCGYC 5 cut(s) 267, 804, 1427, 1526, 1565
Hsp92II CATG 8 cut(s) 235, 691, 817, 844, 965, 1090, 1228, 1525
KasI GGCGCC 2 cut(s) 266, 1525
KflI GGGWCCC 1 cut(s) 541
Kpn2I TCCGGA 2 cut(s) 403, 1111
Ksp22I TGATCA 2 cut(s) 789, 1311
KspI CCGCGG 1 cut(s) 918
Kzo9I GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
LmnI GCTCC 9 cut(s) 7, 126, 246, 348, 451, 708, 876, 910, 1552
Lsp1109I GCAGC 4 cut(s) 673, 1350, 1497, 1542
LweI GCATC 3 cut(s) 134, 433, 1254
MaeII ACGT 9 cut(s) 122, 198, 747, 861, 1101, 1254, 1308, 1458, 1565
MaeIII GTNAC 4 cut(s) 622, 805, 1190, 1454
MalI GATC 6 cut(s) 491, 725, 791, 1110, 1300, 1313
MbiI CCGCTC 1 cut(s) 498
MboI GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
MboII GAAGA 5 cut(s) 340, 388, 1543, 1573, 1588
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 6 cut(s) 243, 657, 870, 881, 907, 1466
MlsI TGGCCA 3 cut(s) 1085, 1400, 1607
MluNI TGGCCA 3 cut(s) 1085, 1400, 1607
Mly113I GGCGCC 2 cut(s) 267, 1526
MlyI GAGTC 3 cut(s) 947, 1361, 1642
MmeI TCCRAC 2 cut(s) 911, 1616
Mox20I TGGCCA 3 cut(s) 1085, 1400, 1607
MroI TCCGGA 2 cut(s) 403, 1111
MscI TGGCCA 3 cut(s) 1085, 1400, 1607
MseI TTAA 1 cut(s) 1131
MslI CAYNNNNRTG 1 cut(s) 812
Msp20I TGGCCA 3 cut(s) 1085, 1400, 1607
MspA1I CMGCKG 1 cut(s) 917
MvaI CCWGG 1 cut(s) 1402
MwoI GCNNNNNNNGC 4 cut(s) 189, 383, 661, 1397
NarI GGCGCC 2 cut(s) 267, 1526
NciI CCSGG 9 cut(s) 506, 568, 608, 901, 1017, 1018, 1093, 1094, 1271
NcoI CCATGG 1 cut(s) 961
NdeII GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
NlaIII CATG 8 cut(s) 235, 691, 817, 844, 965, 1090, 1228, 1525
NmeAIII GCCGAG 2 cut(s) 159, 1539
NmuCI GTSAC 3 cut(s) 622, 805, 1454
NspI RCATGY 2 cut(s) 817, 844
PalAI GGCGCGCC 1 cut(s) 822
PauI GCGCGC 1 cut(s) 822
PceI AGGCCT 1 cut(s) 1405
PciI ACATGT 1 cut(s) 813
PcsI WCGNNNNNNNCGW 5 cut(s) 204, 498, 933, 1046, 1433
PfeI GAWTC 1 cut(s) 1579
Pfl23II CGTACG 1 cut(s) 717
PflFI GACNNNGTC 1 cut(s) 937
PflMI CCANNNNNTGG 1 cut(s) 705
PfoI TCCNGGA 1 cut(s) 566
PleI GAGTC 3 cut(s) 946, 1360, 1641
PluTI GGCGCC 2 cut(s) 270, 1529
PmaCI CACGTG 2 cut(s) 123, 199
PmlI CACGTG 2 cut(s) 123, 199
PpsI GAGTC 3 cut(s) 946, 1360, 1641
Ppu21I YACGTR 2 cut(s) 123, 199
PpuMI RGGWCCY 2 cut(s) 520, 541
PscI ACATGT 1 cut(s) 813
PshAI GACNNNNGTC 2 cut(s) 746, 1094
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 2 cut(s) 520, 541
Psp6I CCWGG 1 cut(s) 1400
PspCI CACGTG 2 cut(s) 123, 199
PspEI GGTNACC 1 cut(s) 1190
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 1 cut(s) 1400
PspLI CGTACG 1 cut(s) 717
PspPPI RGGWCCY 2 cut(s) 520, 541
PsuI RGATCY 1 cut(s) 1108
PsyI GACNNNGTC 1 cut(s) 937
PteI GCGCGC 1 cut(s) 822
RsaI GTAC 4 cut(s) 236, 719, 968, 1364
RsaNI GTAC 4 cut(s) 235, 718, 967, 1363
RseI CAYNNNNRTG 1 cut(s) 812
Rsr2I CGGWCCG 1 cut(s) 933
RsrII CGGWCCG 1 cut(s) 933
SacI GAGCTC 1 cut(s) 243
SacII CCGCGG 1 cut(s) 918
SalI GTCGAC 2 cut(s) 1097, 1567
SaqAI TTAA 1 cut(s) 1131
Sau3AI GATC 6 cut(s) 489, 723, 789, 1108, 1298, 1311
SchI GAGTC 3 cut(s) 947, 1361, 1642
SduI GDGCHC 6 cut(s) 243, 657, 870, 881, 907, 1466
SfaNI GCATC 3 cut(s) 134, 433, 1254
SfoI GGCGCC 2 cut(s) 268, 1527
Sfr303I CCGCGG 1 cut(s) 918
SgrAI CRCCGGYG 2 cut(s) 826, 1247
SgrBI CCGCGG 1 cut(s) 918
SgrDI CGTCGACG 1 cut(s) 1567
SgsI GGCGCGCC 1 cut(s) 822
SinI GGWCC 8 cut(s) 520, 541, 595, 741, 832, 933, 1294, 1620
SmaI CCCGGG 2 cut(s) 1018, 1094
SmiMI CAYNNNNRTG 1 cut(s) 812
SmlI CTYRAG 2 cut(s) 871, 1333
SmoI CTYRAG 2 cut(s) 871, 1333
SseBI AGGCCT 1 cut(s) 1405
SspDI GGCGCC 2 cut(s) 266, 1525
SstI GAGCTC 1 cut(s) 243
StuI AGGCCT 1 cut(s) 1405
StyI CCWWGG 3 cut(s) 961, 1302, 1608
TaaI ACNGT 6 cut(s) 76, 310, 628, 1081, 1235, 1537
TaiI ACGT 9 cut(s) 125, 201, 750, 864, 1104, 1257, 1311, 1461, 1568
TaqI TCGA 9 cut(s) 60, 297, 354, 492, 765, 864, 1061, 1098, 1568
TaqII GACCGA 4 cut(s) 78, 917, 950, 1311
TatI WGTACW 1 cut(s) 1362
TauI GCSGC 8 cut(s) 71, 195, 501, 731, 822, 920, 1376, 1391
TfiI GAWTC 1 cut(s) 1579
Tru1I TTAA 1 cut(s) 1131
Tru9I TTAA 1 cut(s) 1131
TscAI CASTG 2 cut(s) 54, 605
TseFI GTSAC 3 cut(s) 622, 805, 1454
TseI GCWGC 4 cut(s) 661, 1338, 1510, 1555
Tsp45I GTSAC 3 cut(s) 622, 805, 1454
TspDTI ATGAA 3 cut(s) 607, 1241, 1510
TspGWI ACGGA 4 cut(s) 196, 992, 1055, 1490
TspMI CCCGGG 2 cut(s) 1016, 1092
TspRI CASTG 2 cut(s) 54, 605
Tth111I GACNNNGTC 1 cut(s) 937
Van91I CCANNNNNTGG 1 cut(s) 705
VpaK11BI GGWCC 8 cut(s) 520, 541, 595, 741, 832, 933, 1294, 1620
XceI RCATGY 2 cut(s) 817, 844
XmaI CCCGGG 2 cut(s) 1016, 1092
XmiI GTMKAC 3 cut(s) 78, 1098, 1568
ZraI GACGTC 1 cut(s) 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.