RLG00000013740

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000677
Physical Location & Seq
Reverse (-)
37867201 .. 37867663
463 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013740

Sequence Viewer

Length: 351 bp
ATGCACCCGCACCCCATCCCAACTCCTCCGACTCATCCCCGTTCTGCGCTACGGAGTGGTCCACTGCCACAGAGGGCTCCTCATCATCACCGTCAACTCTCTCCTCGACTGGGCTGTACCCAGACAGCTCGTCTATCTCTGGACGCTTCCGATATTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCCCACGCTTCCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCTGAATTTCATGGTGCCGAAGACGGTGGTGTTTATTGAGGAGCTGCCGAAGACGTCGAAATTTGTATGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

117

Amino Acids

13.31

Weight (kDa)

9.91

Isoelectric Point (pI)

60.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 68 - 111 3.5e-07 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 169
AciI CCGC 2 cut(s) 8, 229
AclWI GGATC 2 cut(s) 182, 195
AcsI RAATTY 2 cut(s) 280, 335
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 3 cut(s) 118, 183, 260
AfiI CCNNNNNNNGG 2 cut(s) 110, 169
AhdI GACNNNNNGTC 1 cut(s) 129
AjiI CACGTC 1 cut(s) 223
AluBI AGCT 4 cut(s) 128, 175, 274, 319
AluI AGCT 4 cut(s) 128, 175, 274, 319
AlwI GGATC 2 cut(s) 182, 195
AoxI GGCC 1 cut(s) 233
ApeKI GCWGC 2 cut(s) 274, 319
ApoI RAATTY 2 cut(s) 280, 335
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 80
AvaII GGWCC 1 cut(s) 59
BamHI GGATCC 1 cut(s) 187
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 79
BbsI GAAGAC 2 cut(s) 302, 332
BbvI GCAGC 2 cut(s) 261, 306
BccI CCATC 1 cut(s) 23
BceAI ACGGC 1 cut(s) 182
BfmI CTRYAG 1 cut(s) 262
BisI GCNGC 2 cut(s) 275, 320
BlsI GCNGC 2 cut(s) 276, 321
BmcAI AGTACT 1 cut(s) 260
Bme18I GGWCC 1 cut(s) 59
BmeRI GACNNNNNGTC 1 cut(s) 129
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 3 cut(s) 78, 189, 291
BmrI ACTGGG 1 cut(s) 119
BmuI ACTGGG 1 cut(s) 119
BpiI GAAGAC 2 cut(s) 302, 332
BplI GAGNNNNNCTC 2 cut(s) 64, 96
BpmI CTGGAG 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 329
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 169
Bse1I ACTGG 1 cut(s) 114
BseGI GGATG 2 cut(s) 15, 34
BseLI CCNNNNNNNGG 2 cut(s) 110, 169
BseNI ACTGG 1 cut(s) 114
BseRI GAGGAG 4 cut(s) 15, 69, 93, 329
BseXI GCAGC 2 cut(s) 261, 306
BshFI GGCC 1 cut(s) 235
BshNI GGYRCC 1 cut(s) 289
BsiWI CGTACG 1 cut(s) 181
BslI CCNNNNNNNGG 2 cut(s) 110, 169
BsnI GGCC 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 1 cut(s) 187
BspACI CCGC 2 cut(s) 8, 229
BspANI GGCC 1 cut(s) 235
BspLI GGNNCC 3 cut(s) 78, 189, 291
BspMAI CTGCAG 1 cut(s) 266
BspPI GGATC 2 cut(s) 182, 195
BspT107I GGYRCC 1 cut(s) 289
BsrI ACTGG 1 cut(s) 114
BssMI GATC 1 cut(s) 187
BssNI GRCGYC 1 cut(s) 329
Bst4CI ACNGT 2 cut(s) 92, 301
BstACI GRCGYC 1 cut(s) 329
BstF5I GGATG 2 cut(s) 15, 34
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 1 cut(s) 190
BstMBI GATC 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 262
BstV1I GCAGC 2 cut(s) 261, 306
BstV2I GAAGAC 2 cut(s) 302, 332
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 1 cut(s) 235
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 2 cut(s) 15, 34
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 1 cut(s) 62
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 59
CseI GACGC 1 cut(s) 152
Csp6I GTAC 3 cut(s) 117, 182, 259
CviAII CATG 1 cut(s) 286
CviJI RGCY 9 cut(s) 77, 114, 128, 169, 175, 210, 235, 274, 319
CviKI_1 RGCY 9 cut(s) 77, 114, 128, 169, 175, 210, 235, 274, 319
CviQI GTAC 3 cut(s) 117, 182, 259
DpnI GATC 1 cut(s) 189
DpnII GATC 1 cut(s) 187
DriI GACNNNNNGTC 1 cut(s) 129
Eam1105I GACNNNNNGTC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 79
FaeI CATG 1 cut(s) 289
FaiI YATR 2 cut(s) 287, 343
FatI CATG 1 cut(s) 285
FauI CCCGC 1 cut(s) 15
Fnu4HI GCNGC 2 cut(s) 275, 320
FokI GGATG 2 cut(s) 2, 21
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 275, 320
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 275, 320
GsuI CTGGAG 1 cut(s) 191
HaeIII GGCC 1 cut(s) 235
HgaI GACGC 1 cut(s) 152
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 1 cut(s) 95
HindII GTYRAC 1 cut(s) 95
HinfI GANTC 1 cut(s) 31
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 2 cut(s) 62, 95
Hpy188I TCNGA 2 cut(s) 30, 151
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 2 cut(s) 62, 95
Hpy99I CGWCG 2 cut(s) 241, 334
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 2 cut(s) 92, 301
HpyCH4IV ACGT 2 cut(s) 222, 329
HpyCH4V TGCA 2 cut(s) 4, 264
HpySE526I ACGT 2 cut(s) 222, 329
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 1 cut(s) 47
Kzo9I GATC 1 cut(s) 187
LmnI GCTCC 3 cut(s) 82, 172, 316
LpnPI CCDG 5 cut(s) 95, 125, 134, 155, 224
Lsp1109I GCAGC 2 cut(s) 261, 306
MaeII ACGT 2 cut(s) 222, 329
MalI GATC 1 cut(s) 189
MboI GATC 1 cut(s) 187
MboII GAAGA 2 cut(s) 307, 337
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 2 cut(s) 280, 335
MlyI GAGTC 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 7 cut(s) 36, 66, 90, 114, 225, 260, 307
NdeII GATC 1 cut(s) 187
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 3 cut(s) 78, 189, 291
Pfl23II CGTACG 1 cut(s) 181
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 2 cut(s) 276, 321
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PspLI CGTACG 1 cut(s) 181
PspN4I GGNNCC 3 cut(s) 78, 189, 291
PspPI GGNCC 1 cut(s) 59
PstI CTGCAG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 187
RsaI GTAC 3 cut(s) 118, 183, 260
RsaNI GTAC 3 cut(s) 117, 182, 259
SatI GCNGC 2 cut(s) 275, 320
Sau3AI GATC 1 cut(s) 187
Sau96I GGNCC 1 cut(s) 59
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 1 cut(s) 25
SduI GDGCHC 1 cut(s) 79
SetI ASST 6 cut(s) 130, 177, 225, 276, 321, 332
SfcI CTRYAG 1 cut(s) 262
SinI GGWCC 1 cut(s) 59
Sse9I AATT 2 cut(s) 280, 335
SsiI CCGC 2 cut(s) 8, 229
TaaI ACNGT 2 cut(s) 92, 301
TaiI ACGT 2 cut(s) 225, 332
TaqI TCGA 2 cut(s) 106, 332
TasI AATT 2 cut(s) 280, 335
TatI WGTACW 1 cut(s) 258
TscAI CASTG 1 cut(s) 69
TseI GCWGC 2 cut(s) 274, 319
TspDTI ATGAA 1 cut(s) 274
TspGWI ACGGA 3 cut(s) 67, 189, 254
TspRI CASTG 1 cut(s) 69
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 2 cut(s) 280, 335
ZraI GACGTC 1 cut(s) 330
ZrmI AGTACT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.