Rw3G024990

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000677
Physical Location & Seq
Forward (+)
32652115 .. 32654723
2609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024990.1

Sequence Viewer

Length: 1719 bp
ATGGACCAGCTGAAACCTAGTTCTGCAAACCTCTGCCCTCAAACTCCCCTGGACTTCTTAGACCGAGCAGCGATCGTCTACGGCGACTGCCCCTCCATCATCTACAACTCCACCACCACCTACACCTGGTCCCAAACCCACCGTCGATGTCTCCAGCTGGCTTCCTCCATCTCCACCCTCGTCAAGAAATCCCAGGTCGTCTCCGTGGTGTCCCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTCCCCATGTCGGGAGCCGTCCTCAACAACATCAACACCCGCCTCGACGCCCACACAGTCTCCGTGCTCCTCCAACACAGTGAATCCAAGCTCGTCTTCGTCGACTTCCTCTCCCTCAACCTCGTCCTCCAAGCCCTCTCCTTGTTCCCCTCTCACATCCCCACCCCACCTCTCGTCCTCATCAACGATCATCATCAACCCTCCTCAACCTCCAGTACCGTTGACTTCATAGCGGACACGTACGACAACATGGTGGCCAAAGGCGATCCCGGGTTCGTTTGGGTCCGGCCGGCGAGCGAGTGGGACCCAATGGTGCTGAACTACACTTCAGGGACAACGTCAGCTCCGAAAGGTGCGGTCCACTGCCACAGAGCTCTCTTCAACGTGAGTCTCGTTTCAATCATAGACTGGTCGGTTCCTAAAAATTGTGTGTACCTTTGGACCCTACCGATGTTCCATGCAAATGGGTGGTGCTTCCCGTGGGCCATTGCCGCCGTGGGGGGGACCAACATCTGCCTTCGCAAGTTCGACGGTCCTATAATCTACGATCTGATCCAGAAACACAGAGTGACTCATATGTGCGCCGCGCCTGTCGTTCTCAACATGCTGGTCAACCACCCAAGAACCGACCGGCTTCCGAACCCTGTTTGTATCTTAACCGCCGGAGCTCCGCCTCCTTCGAGTTTGCTGCTCCGGGCCGAGTCACTTGGGTTTGTTGTGAGTCACGGGTACGGGTTGACTGAAACGGCCGGGGTGGTGGTGTCGTGTGCCTGGAAGCAGGAGTGGAATAAGTTTCCGGCGACGGAGCAGGCGAGGTTGAAGGCGAGGCAAGGAGTGAGGACGGTTGGAATGACCGACGCGGATGTGGTGGATCCGGATTCGGGTATGAGCGTGAAACGAAACGGGTCGGATGTTGGAGAAATAGTTATGCGAGGCGGGTGCGTCATGTTGGGTTACCTCAAAGACCCAGAAACGACGAGCGAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTAGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACGCCGCCGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTCAGGCCGAGGCCGACCGAGAAAGAGATAATAGAGTTTACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTCTATGCTGAGAGAATTGCAAAAAATATTGGGTCTGCATCAACGACGAGGTCCAGTCGGTTGTAATAGACGCACTGCAATTTGCTTAGCCCTTTCAAAGAACTTTGTTGTCTACTTGATTCACCATTCAACTCAACTAAAGATTTATCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

572

Amino Acids

62.93

Weight (kDa)

7.25

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 403 2.6e-70 AMP-binding enzyme
AMP-binding_C PF13193 454 - 519 1.6e-12 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1476, 1547
AccI GTMKAC 4 cut(s) 78, 351, 1547, 1677
AccII CGCG 3 cut(s) 836, 1109, 1451
AccIII TCCGGA 1 cut(s) 1123
AclWI GGATC 6 cut(s) 509, 796, 1115, 1128, 1308, 1317
AcoI YGGCCR 4 cut(s) 504, 536, 996, 1404
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 4 cut(s) 297, 1442, 1473, 1544
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 5 cut(s) 233, 466, 491, 683, 980
AfiI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 6 cut(s) 631, 648, 1069, 1525, 1662, 1695
AjiI CACGTC 1 cut(s) 1324
AjnI CCWGG 4 cut(s) 48, 125, 192, 1019
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 8 cut(s) 10, 157, 340, 593, 623, 917, 1469, 1534
AluI AGCT 8 cut(s) 10, 157, 340, 593, 623, 917, 1469, 1534
Alw21I GWGCWC 3 cut(s) 318, 625, 919
Alw26I GTCTC 5 cut(s) 155, 205, 313, 644, 1433
AlwI GGATC 6 cut(s) 509, 796, 1115, 1128, 1308, 1317
Ama87I CYCGRG 1 cut(s) 518
Aor13HI TCCGGA 1 cut(s) 1123
AoxI GGCC 8 cut(s) 504, 536, 732, 945, 996, 1404, 1478, 1484
ApeKI GCWGC 4 cut(s) 68, 937, 1353, 1534
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspLEI GCGC 3 cut(s) 833, 838, 1451
AsuC2I CCSGG 5 cut(s) 519, 520, 944, 1000, 1286
AsuHPI GGTGA 1 cut(s) 1679
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1616
BalI TGGCCA 1 cut(s) 506
BamHI GGATCC 1 cut(s) 1120
BanII GRGCYC 2 cut(s) 625, 919
BbsI GAAGAC 2 cut(s) 337, 1547
Bbv12I GWGCWC 3 cut(s) 318, 625, 919
BbvI GCAGC 4 cut(s) 80, 924, 1365, 1521
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 7 cut(s) 97, 251, 728, 1011, 1376, 1391, 1429
BcgI CGANNNNNNTGC 4 cut(s) 919, 953, 1170, 1204
BciT130I CCWGG 4 cut(s) 50, 127, 194, 1021
BclI TGATCA 1 cut(s) 1326
BcnI CCSGG 5 cut(s) 519, 520, 944, 1000, 1286
BcoDI GTCTC 5 cut(s) 155, 205, 313, 644, 1433
BfaI CTAG 1 cut(s) 18
BglI GCCNNNNNGGC 1 cut(s) 1412
BisI GCNGC 8 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1535
BlpI GCTNAGC 2 cut(s) 1416, 1651
BlsI GCNGC 8 cut(s) 70, 742, 835, 939, 1355, 1390, 1405, 1536
Bme1390I CCNGG 9 cut(s) 50, 127, 194, 519, 520, 944, 1000, 1021, 1286
Bme18I GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1616
BmeT110I CYCGRG 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 1324
BmiI GGNNCC 9 cut(s) 131, 265, 533, 554, 555, 666, 692, 754, 1122
BmrFI CCNGG 9 cut(s) 50, 127, 194, 519, 520, 944, 1000, 1021, 1286
BmsI GCATC 1 cut(s) 1612
BpiI GAAGAC 2 cut(s) 337, 1547
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 3 cut(s) 137, 445, 1319
Bpu1102I GCTNAGC 2 cut(s) 1416, 1651
BpuEI CTTGAG 1 cut(s) 1369
BpuMI CCSGG 5 cut(s) 519, 520, 944, 1000, 1286
Bsa29I ATCGAT 1 cut(s) 1312
BsaAI YACGTR 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 4 cut(s) 297, 1442, 1473, 1544
BsaWI WCCGGW 1 cut(s) 1123
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
Bse118I RCCGGY 3 cut(s) 538, 879, 1262
Bse1I ACTGG 3 cut(s) 462, 662, 1619
Bse3DI GCAATG 1 cut(s) 735
Bse8I GATNNNNATC 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 1123
BseBI CCWGG 4 cut(s) 50, 127, 194, 1021
BseCI ATCGAT 1 cut(s) 1312
BseGI GGATG 3 cut(s) 405, 1117, 1165
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 1 cut(s) 1565
BseNI ACTGG 3 cut(s) 462, 662, 1619
BseRI GAGGAG 2 cut(s) 308, 442
BseX3I CGGCCG 3 cut(s) 536, 996, 1404
BseXI GCAGC 4 cut(s) 80, 924, 1365, 1521
Bsh1236I CGCG 3 cut(s) 836, 1109, 1451
Bsh1285I CGRYCG 6 cut(s) 75, 539, 880, 999, 1407, 1491
BshFI GGCC 8 cut(s) 506, 538, 734, 947, 998, 1406, 1480, 1486
BshVI ATCGAT 1 cut(s) 1312
BsiEI CGRYCG 6 cut(s) 75, 539, 880, 999, 1407, 1491
BsiHKAI GWGCWC 3 cut(s) 318, 625, 919
BsiHKCI CYCGRG 1 cut(s) 518
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BslI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1130
BsmAI GTCTC 5 cut(s) 155, 205, 313, 644, 1433
BsmBI CGTCTC 2 cut(s) 205, 1433
BsmFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BsnI GGCC 8 cut(s) 506, 538, 734, 947, 998, 1406, 1480, 1486
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 3 cut(s) 318, 625, 919
Bsp13I TCCGGA 1 cut(s) 1123
Bsp143I GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
Bsp1720I GCTNAGC 2 cut(s) 1416, 1651
BspANI GGCC 8 cut(s) 506, 538, 734, 947, 998, 1406, 1480, 1486
BspCNI CTCAG 1 cut(s) 1566
BspDI ATCGAT 1 cut(s) 1312
BspEI TCCGGA 1 cut(s) 1123
BspFNI CGCG 3 cut(s) 836, 1109, 1451
BspLI GGNNCC 9 cut(s) 131, 265, 533, 554, 555, 666, 692, 754, 1122
BspPI GGATC 6 cut(s) 509, 796, 1115, 1128, 1308, 1317
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 3 cut(s) 538, 879, 1262
BsrI ACTGG 3 cut(s) 462, 662, 1619
BssAI RCCGGY 3 cut(s) 538, 879, 1262
BssMI GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
BssNI GRCGYC 4 cut(s) 297, 1442, 1473, 1544
BssT1I CCWWGG 1 cut(s) 1317
Bst2UI CCWGG 4 cut(s) 50, 127, 194, 1021
Bst4CI ACNGT 7 cut(s) 143, 307, 329, 469, 782, 1093, 1516
Bst6I CTCTTC 1 cut(s) 632
BstACI GRCGYC 4 cut(s) 297, 1442, 1473, 1544
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 159, 540, 544, 1059
BstDEI CTNAG 4 cut(s) 58, 1416, 1574, 1651
BstDSI CCRYGG 4 cut(s) 204, 728, 744, 1407
BstEII GGTNACC 1 cut(s) 1202
BstF5I GGATG 3 cut(s) 405, 1117, 1165
BstFNI CGCG 3 cut(s) 836, 1109, 1451
BstHHI GCGC 3 cut(s) 833, 838, 1451
BstKTI GATC 9 cut(s) 75, 439, 517, 799, 804, 1123, 1312, 1316, 1329
BstMAI GTCTC 5 cut(s) 155, 205, 313, 644, 1433
BstMBI GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
BstMCI CGRYCG 6 cut(s) 75, 539, 880, 999, 1407, 1491
BstMWI GCNNNNNNNGC 2 cut(s) 740, 1412
BstNI CCWGG 4 cut(s) 50, 127, 194, 1021
BstNSI RCATGY 1 cut(s) 856
BstPI GGTNACC 1 cut(s) 1202
BstSCI CCNGG 9 cut(s) 48, 125, 192, 517, 518, 942, 998, 1019, 1284
BstUI CGCG 3 cut(s) 836, 1109, 1451
BstV1I GCAGC 4 cut(s) 80, 924, 1365, 1521
BstV2I GAAGAC 2 cut(s) 337, 1547
BstX2I RGATCY 1 cut(s) 1120
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 1 cut(s) 1120
BstZI CGGCCG 3 cut(s) 536, 996, 1404
Bsu15I ATCGAT 1 cut(s) 1312
BsuRI GGCC 8 cut(s) 506, 538, 734, 947, 998, 1406, 1480, 1486
BsuTUI ATCGAT 1 cut(s) 1312
BtgI CCRYGG 4 cut(s) 204, 728, 744, 1407
BtrI CACGTC 1 cut(s) 1324
BtsCI GGATG 3 cut(s) 405, 1117, 1165
BtsI GCAGTG 2 cut(s) 610, 1638
BtsIMutI CAGTG 3 cut(s) 334, 610, 1638
Cac8I GCNNGC 4 cut(s) 159, 540, 544, 1059
CfoI GCGC 3 cut(s) 833, 838, 1451
Cfr10I RCCGGY 3 cut(s) 538, 879, 1262
Cfr9I CCCGGG 1 cut(s) 518
ClaI ATCGAT 1 cut(s) 1312
CseI GACGC 5 cut(s) 305, 1115, 1180, 1450, 1644
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 5 cut(s) 232, 465, 490, 682, 979
CviAII CATG 6 cut(s) 229, 256, 499, 707, 853, 1195
CviQI GTAC 5 cut(s) 232, 465, 490, 682, 979
DdeI CTNAG 4 cut(s) 58, 1416, 1574, 1651
DpnI GATC 9 cut(s) 74, 438, 516, 798, 803, 1122, 1311, 1315, 1328
DpnII GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
DraIII CACNNNGTG 1 cut(s) 817
EaeI YGGCCR 4 cut(s) 504, 536, 996, 1404
EagI CGGCCG 3 cut(s) 536, 996, 1404
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
EciI GGCGGA 1 cut(s) 909
Ecl136II GAGCTC 2 cut(s) 623, 917
EclXI CGGCCG 3 cut(s) 536, 996, 1404
Eco130I CCWWGG 1 cut(s) 1317
Eco24I GRGCYC 2 cut(s) 625, 919
Eco47I GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1616
Eco52I CGGCCG 3 cut(s) 536, 996, 1404
Eco53kI GAGCTC 2 cut(s) 623, 917
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 518
Eco91I GGTNACC 1 cut(s) 1202
EcoICRI GAGCTC 2 cut(s) 623, 917
EcoO109I RGGNCCY 1 cut(s) 553
EcoO65I GGTNACC 1 cut(s) 1202
EcoRII CCWGG 4 cut(s) 48, 125, 192, 1019
EcoT14I CCWWGG 1 cut(s) 1317
EcoT38I GRGCYC 2 cut(s) 625, 919
ErhI CCWWGG 1 cut(s) 1317
Esp3I CGTCTC 2 cut(s) 205, 1433
FaeI CATG 6 cut(s) 232, 259, 502, 710, 856, 1198
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
FatI CATG 6 cut(s) 228, 255, 498, 706, 852, 1194
FauI CCCGC 4 cut(s) 232, 296, 1178, 1328
FauNDI CATATG 1 cut(s) 825
FbaI TGATCA 1 cut(s) 1326
FblI GTMKAC 4 cut(s) 78, 351, 1547, 1677
Fnu4HI GCNGC 8 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1535
FokI GGATG 3 cut(s) 392, 1124, 1172
FriOI GRGCYC 2 cut(s) 625, 919
Fsp4HI GCNGC 8 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1535
FspBI CTAG 1 cut(s) 18
GlaI GCGC 3 cut(s) 832, 837, 1450
GluI GCNGC 8 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1535
GsuI CTGGAG 3 cut(s) 137, 445, 1319
HaeIII GGCC 8 cut(s) 506, 538, 734, 947, 998, 1406, 1480, 1486
HgaI GACGC 5 cut(s) 305, 1115, 1180, 1450, 1644
HhaI GCGC 3 cut(s) 833, 838, 1451
Hin1I GRCGYC 4 cut(s) 297, 1442, 1473, 1544
Hin1II CATG 6 cut(s) 232, 259, 502, 710, 856, 1198
Hin6I GCGC 3 cut(s) 831, 836, 1449
HinP1I GCGC 3 cut(s) 831, 836, 1449
HincII GTYRAC 5 cut(s) 352, 472, 862, 987, 1548
HindII GTYRAC 5 cut(s) 352, 472, 862, 987, 1548
HinfI GANTC 9 cut(s) 332, 637, 820, 950, 970, 1127, 1367, 1558, 1684
HphI GGTGA 1 cut(s) 1679
Hpy188I TCNGA 5 cut(s) 597, 801, 888, 1159, 1563
Hpy188III TCNNGA 5 cut(s) 184, 261, 805, 1124, 1298
HpyAV CCTTC 3 cut(s) 776, 936, 1063
HpyCH4III ACNGT 7 cut(s) 143, 307, 329, 469, 782, 1093, 1516
HpyCH4IV ACGT 7 cut(s) 488, 587, 633, 1269, 1323, 1473, 1544
HpyCH4V TGCA 6 cut(s) 26, 710, 1282, 1585, 1603, 1643
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 1412
HpyF3I CTNAG 4 cut(s) 58, 1416, 1574, 1651
HpySE526I ACGT 7 cut(s) 488, 587, 633, 1269, 1323, 1473, 1544
Hsp92I GRCGYC 4 cut(s) 297, 1442, 1473, 1544
Hsp92II CATG 6 cut(s) 232, 259, 502, 710, 856, 1198
HspAI GCGC 3 cut(s) 831, 836, 1449
KflI GGGWCCC 1 cut(s) 553
Kpn2I TCCGGA 1 cut(s) 1123
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 1326
Kzo9I GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
LmnI GCTCC 7 cut(s) 263, 321, 598, 914, 922, 945, 1054
Lsp1109I GCAGC 4 cut(s) 80, 924, 1365, 1521
LweI GCATC 1 cut(s) 1612
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 7 cut(s) 488, 587, 633, 1269, 1323, 1473, 1544
MaeIII GTNAC 4 cut(s) 817, 951, 971, 1202
MalI GATC 9 cut(s) 74, 438, 516, 798, 803, 1122, 1311, 1315, 1328
MboI GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
MboII GAAGA 4 cut(s) 337, 619, 1552, 1567
MflI RGATCY 1 cut(s) 1120
MhlI GDGCHC 3 cut(s) 318, 625, 919
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 3 cut(s) 673, 1580, 1644
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 5 cut(s) 646, 814, 959, 979, 1376
MmeI TCCRAC 4 cut(s) 346, 1075, 1137, 1144
Mox20I TGGCCA 1 cut(s) 506
MroI TCCGGA 1 cut(s) 1123
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 506
MseI TTAA 1 cut(s) 905
MslI CAYNNNNRTG 1 cut(s) 711
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 2 cut(s) 10, 157
MspR9I CCNGG 9 cut(s) 50, 127, 194, 519, 520, 944, 1000, 1021, 1286
MteI GCGCNGCGC 1 cut(s) 834
MvaI CCWGG 4 cut(s) 50, 127, 194, 1021
MvnI CGCG 3 cut(s) 836, 1109, 1451
MwoI GCNNNNNNNGC 2 cut(s) 740, 1412
NaeI GCCGGC 1 cut(s) 540
NciI CCSGG 5 cut(s) 519, 520, 944, 1000, 1286
NdeI CATATG 1 cut(s) 825
NdeII GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 6 cut(s) 232, 259, 502, 710, 856, 1198
NlaIV GGNNCC 9 cut(s) 131, 265, 533, 554, 555, 666, 692, 754, 1122
NmeAIII GCCGAG 2 cut(s) 973, 1506
NmuCI GTSAC 3 cut(s) 817, 951, 971
NspI RCATGY 1 cut(s) 856
PcsI WCGNNNNNNNCGW 7 cut(s) 81, 348, 593, 639, 1058, 1404, 1448
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 4 cut(s) 332, 1127, 1558, 1684
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 2 cut(s) 586, 1614
PkrI GCNGC 8 cut(s) 70, 742, 835, 939, 1355, 1390, 1405, 1536
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 645, 814, 958, 978, 1375
PpsI GAGTC 5 cut(s) 645, 814, 958, 978, 1375
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 553
Psp124BI GAGCTC 2 cut(s) 625, 919
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 4 cut(s) 48, 125, 192, 1019
PspEI GGTNACC 1 cut(s) 1202
PspGI CCWGG 4 cut(s) 48, 125, 192, 1019
PspLI CGTACG 1 cut(s) 489
PspN4I GGNNCC 9 cut(s) 131, 265, 533, 554, 555, 666, 692, 754, 1122
PspPPI RGGWCCY 1 cut(s) 553
PsuI RGATCY 1 cut(s) 1120
PsyI GACNNNGTC 2 cut(s) 586, 1614
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 5 cut(s) 233, 466, 491, 683, 980
RsaNI GTAC 5 cut(s) 232, 465, 490, 682, 979
RseI CAYNNNNRTG 1 cut(s) 711
SacI GAGCTC 2 cut(s) 625, 919
SalI GTCGAC 2 cut(s) 350, 1546
SaqAI TTAA 1 cut(s) 905
SatI GCNGC 8 cut(s) 69, 741, 834, 938, 1354, 1389, 1404, 1535
Sau3AI GATC 9 cut(s) 72, 436, 514, 796, 801, 1120, 1309, 1313, 1326
SchI GAGTC 5 cut(s) 646, 814, 959, 979, 1376
ScrFI CCNGG 9 cut(s) 50, 127, 194, 519, 520, 944, 1000, 1021, 1286
SduI GDGCHC 3 cut(s) 318, 625, 919
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 1612
SgrAI CRCCGGYG 1 cut(s) 1262
SgrDI CGTCGACG 1 cut(s) 1546
SinI GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1616
SmaI CCCGGG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 1348
SmoI CTYRAG 1 cut(s) 1348
Sse9I AATT 3 cut(s) 673, 1580, 1644
SspI AATATT 1 cut(s) 1593
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 2 cut(s) 625, 919
StyD4I CCNGG 9 cut(s) 48, 125, 192, 517, 518, 942, 998, 1019, 1284
StyI CCWWGG 1 cut(s) 1317
TaaI ACNGT 7 cut(s) 143, 307, 329, 469, 782, 1093, 1516
TaiI ACGT 7 cut(s) 491, 590, 636, 1272, 1326, 1476, 1547
TaqI TCGA 7 cut(s) 145, 294, 351, 777, 929, 1312, 1547
TaqII GACCGA 3 cut(s) 78, 1118, 1505
TasI AATT 3 cut(s) 673, 1580, 1644
TauI GCSGC 4 cut(s) 743, 836, 1391, 1406
TfiI GAWTC 4 cut(s) 332, 1127, 1558, 1684
Tru1I TTAA 1 cut(s) 905
Tru9I TTAA 1 cut(s) 905
TscAI CASTG 3 cut(s) 334, 617, 1645
TseFI GTSAC 3 cut(s) 817, 951, 971
TseI GCWGC 4 cut(s) 68, 937, 1353, 1534
Tsp45I GTSAC 3 cut(s) 817, 951, 971
TspDTI ATGAA 1 cut(s) 466
TspGWI ACGGA 4 cut(s) 193, 301, 1067, 1704
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 3 cut(s) 334, 617, 1645
Tth111I GACNNNGTC 2 cut(s) 586, 1614
VpaK11BI GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1616
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 4 cut(s) 78, 351, 1547, 1677
XspI CTAG 1 cut(s) 18
ZraI GACGTC 2 cut(s) 1474, 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.