Rmu_sc0032698.1_g000001

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032698.1
OG0000677
Physical Location & Seq
Reverse (-)
2 .. 762
761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032698.1_g000001.1.cds

Sequence Viewer

Length: 761 bp
atggaccagctgaaacctagttctgcaaacctctgccctcaaactcccctgggcttcttagaccgagcagcgatcgtctacggcgactgcccctccatcatctacaactccaccaccaccttcacctggtcccaaacccaccgtcgatgtctccagctggcttcctccatctccaccctcgtcaagaaatcccaggtcgtctccgtggtgtcccccaacactcccgccatgtacgaactccccttcgccgtccccatgtcgggagccgtcctcaacaacatcaacccccgcctcgacgcccacaccgtctccgtgctcctccaacacagcgaatccaagctcgtcttcgtcgacttcctctccctcaacctcgtcctccaagctctctccttgttcccctctcacatccccgccccacctctcgtcctcatcactgatcatcaacctccctcaacctccaccgttgatttcatagcggacacgtaccacaacatggtggccaaaggcgatcccgggttcgtttgggtccggccggcgagcgagtgggacccaatggtgctgaactacacttcagggacgacgtcagctccgaaaggtgcagtccactgccacagagctctcttcaacgtgagtctcgtttcaatcatagactggactgttcctaaaaattgtgtgtacctttggaccctaccgatgttccatgcaaatgggtggtgcttcccgtgggccatggccgccgtgggggggaccaacatctgcct
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

254

Amino Acids

27.79

Weight (kDa)

6.73

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 584
AccB7I CCANNNNNTGG 1 cut(s) 493
AccI GTMKAC 2 cut(s) 78, 351
AciI CCGC 5 cut(s) 225, 289, 411, 476, 735
AclWI GGATC 1 cut(s) 503
AcoI YGGCCR 3 cut(s) 498, 530, 732
AcuI CTGAAG 1 cut(s) 555
AcyI GRCGYC 2 cut(s) 297, 581
AfaI GTAC 3 cut(s) 233, 485, 677
AfiI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 493, 741, 744
AflIII ACRYGT 1 cut(s) 480
AgsI TTSAA 2 cut(s) 625, 642
AjnI CCWGG 3 cut(s) 48, 125, 192
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 6 cut(s) 10, 157, 340, 383, 587, 617
AluI AGCT 6 cut(s) 10, 157, 340, 383, 587, 617
Alw21I GWGCWC 2 cut(s) 318, 619
Alw26I GTCTC 4 cut(s) 155, 205, 313, 638
AlwI GGATC 1 cut(s) 503
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 4 cut(s) 498, 530, 726, 732
ApeKI GCWGC 1 cut(s) 68
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspS9I GGNCC 7 cut(s) 4, 129, 526, 547, 684, 726, 747
AsuC2I CCSGG 2 cut(s) 513, 514
AsuHPI GGTGA 1 cut(s) 115
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 6 cut(s) 4, 129, 526, 547, 684, 747
BalI TGGCCA 1 cut(s) 500
BanII GRGCYC 1 cut(s) 619
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 2 cut(s) 318, 619
BbvI GCAGC 1 cut(s) 80
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 4 cut(s) 97, 233, 251, 722
BciT130I CCWGG 3 cut(s) 50, 127, 194
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 2 cut(s) 513, 514
BcoDI GTCTC 4 cut(s) 155, 205, 313, 638
BfaI CTAG 1 cut(s) 18
BisI GCNGC 2 cut(s) 69, 735
BlsI GCNGC 2 cut(s) 70, 736
Bme1390I CCNGG 5 cut(s) 50, 127, 194, 513, 514
Bme18I GGWCC 6 cut(s) 4, 129, 526, 547, 684, 747
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 7 cut(s) 4, 129, 526, 547, 684, 726, 747
BmiI GGNNCC 7 cut(s) 131, 265, 527, 548, 549, 686, 748
BmrFI CCNGG 5 cut(s) 50, 127, 194, 513, 514
BpiI GAAGAC 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 1 cut(s) 137
BpuMI CCSGG 2 cut(s) 513, 514
BsaAI YACGTR 1 cut(s) 483
BsaHI GRCGYC 2 cut(s) 297, 581
BsaJI CCNNGG 8 cut(s) 48, 49, 192, 204, 512, 722, 729, 738
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 293, 323, 344, 374, 571, 601
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 259, 260, 493, 741, 744
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 656
BseBI CCWGG 3 cut(s) 50, 127, 194
BseDI CCNNGG 8 cut(s) 48, 49, 192, 204, 512, 722, 729, 738
BseGI GGATG 1 cut(s) 405
BseLI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 493, 741, 744
BseNI ACTGG 1 cut(s) 656
BseRI GAGGAG 1 cut(s) 308
BseX3I CGGCCG 1 cut(s) 530
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 618
Bsh1285I CGRYCG 2 cut(s) 75, 533
BshFI GGCC 4 cut(s) 500, 532, 728, 734
BsiEI CGRYCG 2 cut(s) 75, 533
BsiHKAI GWGCWC 2 cut(s) 318, 619
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 3 cut(s) 513, 529, 533
BslFI GGGAC 5 cut(s) 115, 196, 236, 560, 589
BslI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 493, 741, 744
BsmAI GTCTC 4 cut(s) 155, 205, 313, 638
BsmBI CGTCTC 2 cut(s) 205, 313
BsmFI GGGAC 5 cut(s) 115, 196, 236, 560, 589
BsnI GGCC 4 cut(s) 500, 532, 728, 734
BsoBI CYCGRG 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 318, 619
Bsp143I GATC 3 cut(s) 72, 436, 508
Bsp19I CCATGG 1 cut(s) 729
BspACI CCGC 5 cut(s) 225, 289, 411, 476, 735
BspANI GGCC 4 cut(s) 500, 532, 728, 734
BspLI GGNNCC 7 cut(s) 131, 265, 527, 548, 549, 686, 748
BspPI GGATC 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 1 cut(s) 656
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 8 cut(s) 48, 49, 192, 204, 512, 722, 729, 738
BssMI GATC 3 cut(s) 72, 436, 508
BssNI GRCGYC 2 cut(s) 297, 581
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 3 cut(s) 50, 127, 194
Bst4CI ACNGT 4 cut(s) 143, 307, 463, 658
Bst6I CTCTTC 1 cut(s) 626
BstACI GRCGYC 2 cut(s) 297, 581
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 3 cut(s) 159, 534, 538
BstDEI CTNAG 1 cut(s) 58
BstDSI CCRYGG 4 cut(s) 204, 722, 729, 738
BstF5I GGATG 1 cut(s) 405
BstKTI GATC 3 cut(s) 75, 439, 511
BstMAI GTCTC 4 cut(s) 155, 205, 313, 638
BstMBI GATC 3 cut(s) 72, 436, 508
BstMCI CGRYCG 2 cut(s) 75, 533
BstMWI GCNNNNNNNGC 1 cut(s) 734
BstNI CCWGG 3 cut(s) 50, 127, 194
BstSCI CCNGG 5 cut(s) 48, 125, 192, 511, 512
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 707
BstZI CGGCCG 1 cut(s) 530
BsuRI GGCC 4 cut(s) 500, 532, 728, 734
BtgI CCRYGG 4 cut(s) 204, 722, 729, 738
BtsCI GGATG 1 cut(s) 405
BtsI GCAGTG 1 cut(s) 604
BtsIMutI CAGTG 2 cut(s) 432, 604
Cac8I GCNNGC 3 cut(s) 159, 534, 538
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 7 cut(s) 4, 129, 526, 547, 684, 726, 747
Cfr9I CCCGGG 1 cut(s) 512
CseI GACGC 1 cut(s) 305
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 3 cut(s) 232, 484, 676
CviAII CATG 5 cut(s) 229, 256, 493, 701, 730
CviQI GTAC 3 cut(s) 232, 484, 676
DdeI CTNAG 1 cut(s) 58
DpnI GATC 3 cut(s) 74, 438, 510
DpnII GATC 3 cut(s) 72, 436, 508
EaeI YGGCCR 3 cut(s) 498, 530, 732
EagI CGGCCG 1 cut(s) 530
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
Ecl136II GAGCTC 1 cut(s) 617
EclXI CGGCCG 1 cut(s) 530
Eco130I CCWWGG 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 619
Eco47I GGWCC 6 cut(s) 4, 129, 526, 547, 684, 747
Eco52I CGGCCG 1 cut(s) 530
Eco53kI GAGCTC 1 cut(s) 617
Eco57I CTGAAG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 617
EcoO109I RGGNCCY 1 cut(s) 547
EcoRII CCWGG 3 cut(s) 48, 125, 192
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 619
ErhI CCWWGG 1 cut(s) 729
Esp3I CGTCTC 2 cut(s) 205, 313
FaeI CATG 5 cut(s) 232, 259, 496, 704, 733
FaiI YATR 7 cut(s) 230, 257, 473, 494, 647, 702, 731
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 5 cut(s) 115, 196, 236, 560, 589
FatI CATG 5 cut(s) 228, 255, 492, 700, 729
FauI CCCGC 3 cut(s) 232, 296, 418
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 2 cut(s) 78, 351
Fnu4HI GCNGC 2 cut(s) 69, 735
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 1 cut(s) 619
Fsp4HI GCNGC 2 cut(s) 69, 735
FspBI CTAG 1 cut(s) 18
GluI GCNGC 2 cut(s) 69, 735
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 4 cut(s) 500, 532, 728, 734
HapII CCGG 3 cut(s) 513, 529, 533
HgaI GACGC 1 cut(s) 305
Hin1I GRCGYC 2 cut(s) 297, 581
Hin1II CATG 5 cut(s) 232, 259, 496, 704, 733
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 2 cut(s) 332, 631
HpaII CCGG 3 cut(s) 513, 529, 533
HphI GGTGA 1 cut(s) 115
Hpy166II GTNNAC 4 cut(s) 79, 352, 604, 676
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 2 cut(s) 184, 261
Hpy8I GTNNAC 4 cut(s) 79, 352, 604, 676
Hpy99I CGWCG 4 cut(s) 147, 299, 353, 583
HpyAV CCTTC 2 cut(s) 130, 253
HpyCH4III ACNGT 4 cut(s) 143, 307, 463, 658
HpyCH4IV ACGT 3 cut(s) 482, 581, 627
HpyCH4V TGCA 3 cut(s) 26, 599, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 734
HpyF3I CTNAG 1 cut(s) 58
HpySE526I ACGT 3 cut(s) 482, 581, 627
Hsp92I GRCGYC 2 cut(s) 297, 581
Hsp92II CATG 5 cut(s) 232, 259, 496, 704, 733
KflI GGGWCCC 1 cut(s) 547
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 3 cut(s) 72, 436, 508
LmnI GCTCC 3 cut(s) 263, 321, 592
Lsp1109I GCAGC 1 cut(s) 80
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 3 cut(s) 482, 581, 627
MalI GATC 3 cut(s) 74, 438, 510
MboI GATC 3 cut(s) 72, 436, 508
MboII GAAGA 2 cut(s) 337, 613
MhlI GDGCHC 2 cut(s) 318, 619
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 1 cut(s) 667
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 346
Mox20I TGGCCA 1 cut(s) 500
MroNI GCCGGC 1 cut(s) 532
MscI TGGCCA 1 cut(s) 500
MslI CAYNNNNRTG 1 cut(s) 705
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 2 cut(s) 10, 157
MspI CCGG 3 cut(s) 513, 529, 533
MspR9I CCNGG 5 cut(s) 50, 127, 194, 513, 514
MvaI CCWGG 3 cut(s) 50, 127, 194
MwoI GCNNNNNNNGC 1 cut(s) 734
NaeI GCCGGC 1 cut(s) 534
NciI CCSGG 2 cut(s) 513, 514
NcoI CCATGG 1 cut(s) 729
NdeII GATC 3 cut(s) 72, 436, 508
NgoMIV GCCGGC 1 cut(s) 532
NlaIII CATG 5 cut(s) 232, 259, 496, 704, 733
NlaIV GGNNCC 7 cut(s) 131, 265, 527, 548, 549, 686, 748
PasI CCCWGGG 1 cut(s) 49
PcsI WCGNNNNNNNCGW 5 cut(s) 81, 303, 348, 587, 633
PdiI GCCGGC 1 cut(s) 534
PfeI GAWTC 1 cut(s) 332
PflFI GACNNNGTC 1 cut(s) 580
PflMI CCANNNNNTGG 1 cut(s) 493
PkrI GCNGC 2 cut(s) 70, 736
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 639
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 1 cut(s) 547
Psp124BI GAGCTC 1 cut(s) 619
Psp5II RGGWCCY 1 cut(s) 547
Psp6I CCWGG 3 cut(s) 48, 125, 192
PspGI CCWGG 3 cut(s) 48, 125, 192
PspN4I GGNNCC 7 cut(s) 131, 265, 527, 548, 549, 686, 748
PspPI GGNCC 7 cut(s) 4, 129, 526, 547, 684, 726, 747
PspPPI RGGWCCY 1 cut(s) 547
PsyI GACNNNGTC 1 cut(s) 580
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 3 cut(s) 233, 485, 677
RsaNI GTAC 3 cut(s) 232, 484, 676
RseI CAYNNNNRTG 1 cut(s) 705
SacI GAGCTC 1 cut(s) 619
SalI GTCGAC 1 cut(s) 350
SatI GCNGC 2 cut(s) 69, 735
Sau3AI GATC 3 cut(s) 72, 436, 508
Sau96I GGNCC 7 cut(s) 4, 129, 526, 547, 684, 726, 747
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 5 cut(s) 50, 127, 194, 513, 514
SduI GDGCHC 2 cut(s) 318, 619
SexAI ACCWGGT 1 cut(s) 125
SinI GGWCC 6 cut(s) 4, 129, 526, 547, 684, 747
SmaI CCCGGG 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 705
Sse9I AATT 1 cut(s) 667
SsiI CCGC 5 cut(s) 225, 289, 411, 476, 735
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 1 cut(s) 619
StyD4I CCNGG 5 cut(s) 48, 125, 192, 511, 512
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 4 cut(s) 143, 307, 463, 658
TaiI ACGT 3 cut(s) 485, 584, 630
TaqI TCGA 3 cut(s) 145, 294, 351
TaqII GACCGA 1 cut(s) 78
TasI AATT 1 cut(s) 667
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 1 cut(s) 332
TscAI CASTG 2 cut(s) 439, 611
TseI GCWGC 1 cut(s) 68
TspDTI ATGAA 1 cut(s) 460
TspGWI ACGGA 2 cut(s) 193, 301
TspMI CCCGGG 1 cut(s) 512
TspRI CASTG 2 cut(s) 439, 611
Tth111I GACNNNGTC 1 cut(s) 580
Van91I CCANNNNNTGG 1 cut(s) 493
VpaK11BI GGWCC 6 cut(s) 4, 129, 526, 547, 684, 747
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmaI CCCGGG 1 cut(s) 512
XmiI GTMKAC 2 cut(s) 78, 351
XspI CTAG 1 cut(s) 18
ZraI GACGTC 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.