Acyl-activating enzyme
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 92, 962 |
| Acc16I | TGCGCA | 1 cut(s) | 829 |
| AccB1I | GGYRCC | 1 cut(s) | 337 |
| AccI | GTMKAC | 4 cut(s) | 962, 1016, 1092, 1114 |
| AccII | CGCG | 1 cut(s) | 335 |
| AccIII | TCCGGA | 1 cut(s) | 625 |
| AciI | CCGC | 8 cut(s) | 271, 335, 383, 509, 768, 782, 1102, 1243 |
| AclWI | GGATC | 4 cut(s) | 11, 298, 617, 630 |
| AcoI | YGGCCR | 4 cut(s) | 6, 783, 1122, 1244 |
| AcsI | RAATTY | 1 cut(s) | 910 |
| AcuI | CTGAAG | 1 cut(s) | 944 |
| AcyI | GRCGYC | 4 cut(s) | 89, 338, 821, 959 |
| AdeI | CACNNNGTG | 1 cut(s) | 319 |
| AfaI | GTAC | 3 cut(s) | 111, 185, 482 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 632, 1102 |
| AflIII | ACRYGT | 4 cut(s) | 75, 261, 327, 354 |
| AgsI | TTSAA | 4 cut(s) | 133, 150, 571, 940 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 949 |
| AjiI | CACGTC | 2 cut(s) | 78, 610 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 735, 767 |
| AluBI | AGCT | 9 cut(s) | 95, 125, 419, 848, 949, 1190, 1212, 1299, 1344 |
| AluI | AGCT | 9 cut(s) | 95, 125, 419, 848, 949, 1190, 1212, 1299, 1344 |
| Alw21I | GWGCWC | 2 cut(s) | 127, 421 |
| Alw26I | GTCTC | 6 cut(s) | 146, 459, 812, 1089, 1111, 1144 |
| AlwI | GGATC | 4 cut(s) | 11, 298, 617, 630 |
| Ama87I | CYCGRG | 3 cut(s) | 20, 370, 458 |
| Aor13HI | TCCGGA | 1 cut(s) | 625 |
| AoxI | GGCC | 9 cut(s) | 6, 38, 234, 447, 783, 857, 863, 1122, 1244 |
| ApeKI | GCWGC | 1 cut(s) | 242 |
| ApoI | RAATTY | 1 cut(s) | 910 |
| AspLEI | GCGC | 3 cut(s) | 335, 340, 830 |
| AspS9I | GGNCC | 7 cut(s) | 55, 192, 234, 255, 284, 447, 1032 |
| AsuC2I | CCSGG | 4 cut(s) | 21, 22, 446, 741 |
| AsuHPI | GGTGA | 1 cut(s) | 562 |
| AsuNHI | GCTAGC | 1 cut(s) | 1286 |
| AvaI | CYCGRG | 3 cut(s) | 20, 370, 458 |
| AvaII | GGWCC | 5 cut(s) | 55, 192, 255, 284, 1032 |
| BalI | TGGCCA | 1 cut(s) | 8 |
| BamHI | GGATCC | 1 cut(s) | 622 |
| BanI | GGYRCC | 1 cut(s) | 337 |
| BanII | GRGCYC | 2 cut(s) | 127, 421 |
| BauI | CACGAG | 1 cut(s) | 229 |
| BbsI | GAAGAC | 2 cut(s) | 932, 962 |
| Bbv12I | GWGCWC | 2 cut(s) | 127, 421 |
| BbvI | GCAGC | 1 cut(s) | 229 |
| BceAI | ACGGC | 4 cut(s) | 513, 755, 770, 808 |
| BcnI | CCSGG | 4 cut(s) | 21, 22, 446, 741 |
| BcoDI | GTCTC | 6 cut(s) | 146, 459, 812, 1089, 1111, 1144 |
| BfaI | CTAG | 2 cut(s) | 1287, 1354 |
| BfoI | RGCGCY | 1 cut(s) | 341 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 791, 1252 |
| BisI | GCNGC | 5 cut(s) | 243, 336, 768, 783, 1244 |
| BlpI | GCTNAGC | 1 cut(s) | 795 |
| BlsI | GCNGC | 5 cut(s) | 244, 337, 769, 784, 1245 |
| Bme1390I | CCNGG | 4 cut(s) | 21, 22, 446, 741 |
| Bme18I | GGWCC | 5 cut(s) | 55, 192, 255, 284, 1032 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 949 |
| BmeT110I | CYCGRG | 3 cut(s) | 20, 370, 458 |
| BmgBI | CACGTC | 2 cut(s) | 78, 610 |
| BmgT120I | GGNCC | 7 cut(s) | 55, 192, 234, 255, 284, 447, 1032 |
| BmiI | GGNNCC | 7 cut(s) | 56, 57, 168, 194, 256, 339, 624 |
| BmrFI | CCNGG | 4 cut(s) | 21, 22, 446, 741 |
| BmsI | GCATC | 1 cut(s) | 429 |
| BmtI | GCTAGC | 1 cut(s) | 1290 |
| BpiI | GAAGAC | 2 cut(s) | 932, 962 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 426, 458 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 795 |
| BpuMI | CCSGG | 4 cut(s) | 21, 22, 446, 741 |
| BsaBI | GATNNNNATC | 1 cut(s) | 401 |
| BsaHI | GRCGYC | 4 cut(s) | 89, 338, 821, 959 |
| BsaI | GGTCTC | 3 cut(s) | 1089, 1111, 1144 |
| BsaJI | CCNNGG | 4 cut(s) | 20, 237, 786, 860 |
| BsaWI | WCCGGW | 4 cut(s) | 547, 625, 1087, 1109 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 79, 109, 809, 839, 1069, 1099, 1124, 1154 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 632, 1102 |
| Bse118I | RCCGGY | 1 cut(s) | 717 |
| Bse1I | ACTGG | 3 cut(s) | 164, 488, 1035 |
| Bse8I | GATNNNNATC | 1 cut(s) | 401 |
| BseAI | TCCGGA | 1 cut(s) | 625 |
| BseDI | CCNNGG | 4 cut(s) | 20, 237, 786, 860 |
| BseGI | GGATG | 1 cut(s) | 619 |
| BseJI | GATNNNNATC | 1 cut(s) | 401 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 632, 1102 |
| BseMII | CTCAG | 1 cut(s) | 980 |
| BseNI | ACTGG | 3 cut(s) | 164, 488, 1035 |
| BseRI | GAGGAG | 1 cut(s) | 1060 |
| BseX3I | CGGCCG | 1 cut(s) | 783 |
| BseXI | GCAGC | 1 cut(s) | 229 |
| BseYI | CCCAGC | 1 cut(s) | 500 |
| BsgI | GTGCAG | 1 cut(s) | 126 |
| Bsh1236I | CGCG | 1 cut(s) | 335 |
| Bsh1285I | CGRYCG | 5 cut(s) | 749, 786, 1092, 1114, 1147 |
| BshFI | GGCC | 9 cut(s) | 8, 40, 236, 449, 785, 859, 865, 1124, 1246 |
| BshNI | GGYRCC | 1 cut(s) | 337 |
| BsiEI | CGRYCG | 5 cut(s) | 749, 786, 1092, 1114, 1147 |
| BsiHKAI | GWGCWC | 2 cut(s) | 127, 421 |
| BsiHKCI | CYCGRG | 3 cut(s) | 20, 370, 458 |
| BslFI | GGGAC | 3 cut(s) | 68, 97, 268 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 632, 1102 |
| BsmAI | GTCTC | 6 cut(s) | 146, 459, 812, 1089, 1111, 1144 |
| BsmBI | CGTCTC | 1 cut(s) | 812 |
| BsmFI | GGGAC | 3 cut(s) | 68, 97, 268 |
| BsnI | GGCC | 9 cut(s) | 8, 40, 236, 449, 785, 859, 865, 1124, 1246 |
| Bso31I | GGTCTC | 3 cut(s) | 1089, 1111, 1144 |
| BsoBI | CYCGRG | 3 cut(s) | 20, 370, 458 |
| Bsp1286I | GDGCHC | 2 cut(s) | 127, 421 |
| Bsp13I | TCCGGA | 1 cut(s) | 625 |
| Bsp143I | GATC | 4 cut(s) | 16, 298, 303, 622 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 795 |
| Bsp19I | CCATGG | 1 cut(s) | 237 |
| BspACI | CCGC | 8 cut(s) | 271, 335, 383, 509, 768, 782, 1102, 1243 |
| BspANI | GGCC | 9 cut(s) | 8, 40, 236, 449, 785, 859, 865, 1124, 1246 |
| BspCNI | CTCAG | 1 cut(s) | 981 |
| BspEI | TCCGGA | 1 cut(s) | 625 |
| BspFNI | CGCG | 1 cut(s) | 335 |
| BspLI | GGNNCC | 7 cut(s) | 56, 57, 168, 194, 256, 339, 624 |
| BspOI | GCTAGC | 1 cut(s) | 1290 |
| BspPI | GGATC | 4 cut(s) | 11, 298, 617, 630 |
| BspT107I | GGYRCC | 1 cut(s) | 337 |
| BspTNI | GGTCTC | 3 cut(s) | 1089, 1111, 1144 |
| BsrFI | RCCGGY | 1 cut(s) | 717 |
| BsrI | ACTGG | 3 cut(s) | 164, 488, 1035 |
| BssAI | RCCGGY | 1 cut(s) | 717 |
| BssECI | CCNNGG | 4 cut(s) | 20, 237, 786, 860 |
| BssMI | GATC | 4 cut(s) | 16, 298, 303, 622 |
| BssNI | GRCGYC | 4 cut(s) | 89, 338, 821, 959 |
| BssSI | CACGAG | 1 cut(s) | 229 |
| BssT1I | CCWWGG | 1 cut(s) | 237 |
| Bst2BI | CACGAG | 1 cut(s) | 229 |
| Bst4CI | ACNGT | 5 cut(s) | 284, 595, 747, 931, 1217 |
| Bst6I | CTCTTC | 1 cut(s) | 134 |
| BstACI | GRCGYC | 4 cut(s) | 89, 338, 821, 959 |
| BstC8I | GCNNGC | 4 cut(s) | 42, 46, 1278, 1288 |
| BstDEI | CTNAG | 2 cut(s) | 795, 989 |
| BstDSI | CCRYGG | 2 cut(s) | 237, 786 |
| BstEII | GGTNACC | 1 cut(s) | 657 |
| BstF5I | GGATG | 1 cut(s) | 619 |
| BstFNI | CGCG | 1 cut(s) | 335 |
| BstH2I | RGCGCY | 1 cut(s) | 341 |
| BstHHI | GCGC | 3 cut(s) | 335, 340, 830 |
| BstKTI | GATC | 4 cut(s) | 19, 301, 306, 625 |
| BstMAI | GTCTC | 6 cut(s) | 146, 459, 812, 1089, 1111, 1144 |
| BstMBI | GATC | 4 cut(s) | 16, 298, 303, 622 |
| BstMCI | CGRYCG | 5 cut(s) | 749, 786, 1092, 1114, 1147 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 242, 506, 791, 1209, 1252, 1296 |
| BstNSI | RCATGY | 3 cut(s) | 265, 331, 358 |
| BstPI | GGTNACC | 1 cut(s) | 657 |
| BstSCI | CCNGG | 4 cut(s) | 19, 20, 444, 739 |
| BstUI | CGCG | 1 cut(s) | 335 |
| BstV1I | GCAGC | 1 cut(s) | 229 |
| BstV2I | GAAGAC | 2 cut(s) | 932, 962 |
| BstX2I | RGATCY | 1 cut(s) | 622 |
| BstYI | RGATCY | 1 cut(s) | 622 |
| BstZI | CGGCCG | 1 cut(s) | 783 |
| BsuRI | GGCC | 9 cut(s) | 8, 40, 236, 449, 785, 859, 865, 1124, 1246 |
| BtgI | CCRYGG | 2 cut(s) | 237, 786 |
| BtgZI | GCGATG | 1 cut(s) | 736 |
| BtrI | CACGTC | 2 cut(s) | 78, 610 |
| BtsCI | GGATG | 1 cut(s) | 619 |
| BtsI | GCAGTG | 1 cut(s) | 112 |
| BtsIMutI | CAGTG | 1 cut(s) | 112 |
| Cac8I | GCNNGC | 4 cut(s) | 42, 46, 1278, 1288 |
| CfoI | GCGC | 3 cut(s) | 335, 340, 830 |
| Cfr10I | RCCGGY | 1 cut(s) | 717 |
| Cfr13I | GGNCC | 7 cut(s) | 55, 192, 234, 255, 284, 447, 1032 |
| Cfr9I | CCCGGG | 1 cut(s) | 20 |
| CseI | GACGC | 1 cut(s) | 829 |
| Csp6I | GTAC | 3 cut(s) | 110, 184, 481 |
| CviAII | CATG | 6 cut(s) | 209, 238, 262, 328, 355, 916 |
| CviQI | GTAC | 3 cut(s) | 110, 184, 481 |
| DdeI | CTNAG | 2 cut(s) | 795, 989 |
| DinI | GGCGCC | 1 cut(s) | 339 |
| DpnI | GATC | 4 cut(s) | 18, 300, 305, 624 |
| DpnII | GATC | 4 cut(s) | 16, 298, 303, 622 |
| DraIII | CACNNNGTG | 1 cut(s) | 319 |
| DriI | GACNNNNNGTC | 1 cut(s) | 949 |
| EaeI | YGGCCR | 4 cut(s) | 6, 783, 1122, 1244 |
| EagI | CGGCCG | 1 cut(s) | 783 |
| Eam1104I | CTCTTC | 1 cut(s) | 134 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 949 |
| EarI | CTCTTC | 1 cut(s) | 134 |
| Ecl136II | GAGCTC | 2 cut(s) | 125, 419 |
| EclXI | CGGCCG | 1 cut(s) | 783 |
| Eco130I | CCWWGG | 1 cut(s) | 237 |
| Eco24I | GRGCYC | 2 cut(s) | 127, 421 |
| Eco31I | GGTCTC | 3 cut(s) | 1089, 1111, 1144 |
| Eco47I | GGWCC | 5 cut(s) | 55, 192, 255, 284, 1032 |
| Eco52I | CGGCCG | 1 cut(s) | 783 |
| Eco53kI | GAGCTC | 2 cut(s) | 125, 419 |
| Eco57I | CTGAAG | 1 cut(s) | 944 |
| Eco88I | CYCGRG | 3 cut(s) | 20, 370, 458 |
| Eco91I | GGTNACC | 1 cut(s) | 657 |
| EcoICRI | GAGCTC | 2 cut(s) | 125, 419 |
| EcoO109I | RGGNCCY | 1 cut(s) | 55 |
| EcoO65I | GGTNACC | 1 cut(s) | 657 |
| EcoT14I | CCWWGG | 1 cut(s) | 237 |
| EcoT38I | GRGCYC | 2 cut(s) | 127, 421 |
| EgeI | GGCGCC | 1 cut(s) | 339 |
| EheI | GGCGCC | 1 cut(s) | 339 |
| ErhI | CCWWGG | 1 cut(s) | 237 |
| Esp3I | CGTCTC | 1 cut(s) | 812 |
| FaeI | CATG | 6 cut(s) | 212, 241, 265, 331, 358, 919 |
| FaqI | GGGAC | 3 cut(s) | 68, 97, 268 |
| FatI | CATG | 6 cut(s) | 208, 237, 261, 327, 354, 915 |
| FauI | CCCGC | 2 cut(s) | 390, 1095 |
| FblI | GTMKAC | 4 cut(s) | 962, 1016, 1092, 1114 |
| Fnu4HI | GCNGC | 5 cut(s) | 243, 336, 768, 783, 1244 |
| FokI | GGATG | 1 cut(s) | 626 |
| FriOI | GRGCYC | 2 cut(s) | 127, 421 |
| Fsp4HI | GCNGC | 5 cut(s) | 243, 336, 768, 783, 1244 |
| FspAI | RTGCGCAY | 1 cut(s) | 829 |
| FspBI | CTAG | 2 cut(s) | 1287, 1354 |
| FspI | TGCGCA | 1 cut(s) | 829 |
| GlaI | GCGC | 3 cut(s) | 334, 339, 829 |
| GluI | GCNGC | 5 cut(s) | 243, 336, 768, 783, 1244 |
| GsaI | CCCAGC | 1 cut(s) | 504 |
| HaeII | RGCGCY | 1 cut(s) | 341 |
| HaeIII | GGCC | 9 cut(s) | 8, 40, 236, 449, 785, 859, 865, 1124, 1246 |
| HgaI | GACGC | 1 cut(s) | 829 |
| HhaI | GCGC | 3 cut(s) | 335, 340, 830 |
| Hin1I | GRCGYC | 4 cut(s) | 89, 338, 821, 959 |
| Hin1II | CATG | 6 cut(s) | 212, 241, 265, 331, 358, 919 |
| Hin6I | GCGC | 3 cut(s) | 333, 338, 828 |
| HinP1I | GCGC | 3 cut(s) | 333, 338, 828 |
| HincII | GTYRAC | 7 cut(s) | 364, 489, 963, 1023, 1052, 1093, 1115 |
| HindII | GTYRAC | 7 cut(s) | 364, 489, 963, 1023, 1052, 1093, 1115 |
| HinfI | GANTC | 6 cut(s) | 139, 322, 452, 472, 629, 973 |
| HphI | GGTGA | 1 cut(s) | 562 |
| Hpy188I | TCNGA | 9 cut(s) | 99, 303, 390, 978, 1132, 1144, 1155, 1224, 1306 |
| Hpy188III | TCNNGA | 2 cut(s) | 307, 626 |
| Hpy99I | CGWCG | 7 cut(s) | 91, 284, 611, 683, 964, 967, 1136 |
| HpyAV | CCTTC | 2 cut(s) | 565, 868 |
| HpyCH4III | ACNGT | 5 cut(s) | 284, 595, 747, 931, 1217 |
| HpyCH4IV | ACGT | 6 cut(s) | 77, 89, 135, 609, 959, 1019 |
| HpyCH4V | TGCA | 3 cut(s) | 107, 431, 737 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 242, 506, 791, 1209, 1252, 1296 |
| HpyF3I | CTNAG | 2 cut(s) | 795, 989 |
| HpySE526I | ACGT | 6 cut(s) | 77, 89, 135, 609, 959, 1019 |
| Hsp92I | GRCGYC | 4 cut(s) | 89, 338, 821, 959 |
| Hsp92II | CATG | 6 cut(s) | 212, 241, 265, 331, 358, 919 |
| HspAI | GCGC | 3 cut(s) | 333, 338, 828 |
| KasI | GGCGCC | 1 cut(s) | 337 |
| KflI | GGGWCCC | 1 cut(s) | 55 |
| Kpn2I | TCCGGA | 1 cut(s) | 625 |
| Kzo9I | GATC | 4 cut(s) | 16, 298, 303, 622 |
| LmnI | GCTCC | 4 cut(s) | 100, 416, 424, 447 |
| Lsp1109I | GCAGC | 1 cut(s) | 229 |
| LweI | GCATC | 1 cut(s) | 429 |
| MaeI | CTAG | 2 cut(s) | 1287, 1354 |
| MaeII | ACGT | 6 cut(s) | 77, 89, 135, 609, 959, 1019 |
| MaeIII | GTNAC | 5 cut(s) | 319, 473, 550, 657, 1334 |
| MalI | GATC | 4 cut(s) | 18, 300, 305, 624 |
| MboI | GATC | 4 cut(s) | 16, 298, 303, 622 |
| MboII | GAAGA | 4 cut(s) | 121, 937, 967, 982 |
| MflI | RGATCY | 1 cut(s) | 622 |
| MhlI | GDGCHC | 2 cut(s) | 127, 421 |
| MlsI | TGGCCA | 1 cut(s) | 8 |
| MluCI | AATT | 3 cut(s) | 175, 910, 1194 |
| MluNI | TGGCCA | 1 cut(s) | 8 |
| Mly113I | GGCGCC | 1 cut(s) | 338 |
| MlyI | GAGTC | 4 cut(s) | 148, 316, 461, 481 |
| MmeI | TCCRAC | 5 cut(s) | 577, 1100, 1155, 1167, 1178 |
| Mox20I | TGGCCA | 1 cut(s) | 8 |
| MroI | TCCGGA | 1 cut(s) | 625 |
| MscI | TGGCCA | 1 cut(s) | 8 |
| MseI | TTAA | 1 cut(s) | 407 |
| Msp20I | TGGCCA | 1 cut(s) | 8 |
| MspR9I | CCNGG | 4 cut(s) | 21, 22, 446, 741 |
| MteI | GCGCNGCGC | 1 cut(s) | 336 |
| MvnI | CGCG | 1 cut(s) | 335 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 242, 506, 791, 1209, 1252, 1296 |
| NarI | GGCGCC | 1 cut(s) | 338 |
| NciI | CCSGG | 4 cut(s) | 21, 22, 446, 741 |
| NcoI | CCATGG | 1 cut(s) | 237 |
| NdeII | GATC | 4 cut(s) | 16, 298, 303, 622 |
| NheI | GCTAGC | 1 cut(s) | 1286 |
| NlaIII | CATG | 6 cut(s) | 212, 241, 265, 331, 358, 919 |
| NlaIV | GGNNCC | 7 cut(s) | 56, 57, 168, 194, 256, 339, 624 |
| NmeAIII | GCCGAG | 2 cut(s) | 475, 885 |
| NmuCI | GTSAC | 4 cut(s) | 319, 473, 550, 1334 |
| NsbI | TGCGCA | 1 cut(s) | 829 |
| NspI | RCATGY | 3 cut(s) | 265, 331, 358 |
| PciI | ACATGT | 3 cut(s) | 261, 327, 354 |
| PcsI | WCGNNNNNNNCGW | 5 cut(s) | 83, 95, 783, 959, 1140 |
| PfeI | GAWTC | 2 cut(s) | 629, 973 |
| PflFI | GACNNNGTC | 2 cut(s) | 88, 1136 |
| PkrI | GCNGC | 5 cut(s) | 244, 337, 769, 784, 1245 |
| PleI | GAGTC | 4 cut(s) | 147, 316, 460, 480 |
| PluTI | GGCGCC | 1 cut(s) | 341 |
| PpsI | GAGTC | 4 cut(s) | 147, 316, 460, 480 |
| PpuMI | RGGWCCY | 1 cut(s) | 55 |
| PscI | ACATGT | 3 cut(s) | 261, 327, 354 |
| Psp124BI | GAGCTC | 2 cut(s) | 127, 421 |
| Psp5II | RGGWCCY | 1 cut(s) | 55 |
| PspEI | GGTNACC | 1 cut(s) | 657 |
| PspFI | CCCAGC | 1 cut(s) | 500 |
| PspN4I | GGNNCC | 7 cut(s) | 56, 57, 168, 194, 256, 339, 624 |
| PspPI | GGNCC | 7 cut(s) | 55, 192, 234, 255, 284, 447, 1032 |
| PspPPI | RGGWCCY | 1 cut(s) | 55 |
| PsuI | RGATCY | 1 cut(s) | 622 |
| PsyI | GACNNNGTC | 2 cut(s) | 88, 1136 |
| RsaI | GTAC | 3 cut(s) | 111, 185, 482 |
| RsaNI | GTAC | 3 cut(s) | 110, 184, 481 |
| SacI | GAGCTC | 2 cut(s) | 127, 421 |
| SalI | GTCGAC | 3 cut(s) | 961, 1091, 1113 |
| SaqAI | TTAA | 1 cut(s) | 407 |
| SatI | GCNGC | 5 cut(s) | 243, 336, 768, 783, 1244 |
| Sau3AI | GATC | 4 cut(s) | 16, 298, 303, 622 |
| Sau96I | GGNCC | 7 cut(s) | 55, 192, 234, 255, 284, 447, 1032 |
| SchI | GAGTC | 4 cut(s) | 148, 316, 461, 481 |
| ScrFI | CCNGG | 4 cut(s) | 21, 22, 446, 741 |
| SduI | GDGCHC | 2 cut(s) | 127, 421 |
| SfaNI | GCATC | 1 cut(s) | 429 |
| SfoI | GGCGCC | 1 cut(s) | 339 |
| SgrAI | CRCCGGYG | 1 cut(s) | 717 |
| SgrDI | CGTCGACG | 1 cut(s) | 961 |
| SinI | GGWCC | 5 cut(s) | 55, 192, 255, 284, 1032 |
| SmaI | CCCGGG | 1 cut(s) | 22 |
| Sse9I | AATT | 3 cut(s) | 175, 910, 1194 |
| SsiI | CCGC | 8 cut(s) | 271, 335, 383, 509, 768, 782, 1102, 1243 |
| SspDI | GGCGCC | 1 cut(s) | 337 |
| SspI | AATATT | 1 cut(s) | 1009 |
| SspMI | CTAG | 2 cut(s) | 1287, 1354 |
| SstI | GAGCTC | 2 cut(s) | 127, 421 |
| StyD4I | CCNGG | 4 cut(s) | 19, 20, 444, 739 |
| StyI | CCWWGG | 1 cut(s) | 237 |
| TaaI | ACNGT | 5 cut(s) | 284, 595, 747, 931, 1217 |
| TaiI | ACGT | 6 cut(s) | 80, 92, 138, 612, 962, 1022 |
| TaqI | TCGA | 6 cut(s) | 279, 953, 962, 1039, 1092, 1114 |
| TaqII | GACCGA | 2 cut(s) | 620, 1161 |
| TasI | AATT | 3 cut(s) | 175, 910, 1194 |
| TatI | WGTACW | 1 cut(s) | 109 |
| TauI | GCSGC | 4 cut(s) | 338, 770, 785, 1246 |
| TfiI | GAWTC | 2 cut(s) | 629, 973 |
| Tru1I | TTAA | 1 cut(s) | 407 |
| Tru9I | TTAA | 1 cut(s) | 407 |
| TscAI | CASTG | 1 cut(s) | 119 |
| TseFI | GTSAC | 4 cut(s) | 319, 473, 550, 1334 |
| TseI | GCWGC | 1 cut(s) | 242 |
| Tsp45I | GTSAC | 4 cut(s) | 319, 473, 550, 1334 |
| TspDTI | ATGAA | 2 cut(s) | 225, 904 |
| TspMI | CCCGGG | 1 cut(s) | 20 |
| TspRI | CASTG | 1 cut(s) | 119 |
| Tth111I | GACNNNGTC | 2 cut(s) | 88, 1136 |
| VpaK11BI | GGWCC | 5 cut(s) | 55, 192, 255, 284, 1032 |
| XapI | RAATTY | 1 cut(s) | 910 |
| XceI | RCATGY | 3 cut(s) | 265, 331, 358 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 48, 244 |
| XmaI | CCCGGG | 1 cut(s) | 20 |
| XmiI | GTMKAC | 4 cut(s) | 962, 1016, 1092, 1114 |
| XspI | CTAG | 2 cut(s) | 1287, 1354 |
| ZraI | GACGTC | 2 cut(s) | 90, 960 |