Rh3BG318300

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000677
Physical Location & Seq
Reverse (-)
32639285 .. 32639704
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG318300.1

Sequence Viewer

Length: 420 bp
ATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTTGAGATCAAGGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACTCCGCCGTGAACGAGGCGGCCGTGGTGGCGAGGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCCGCATTTCATGGTGCCGAAGACGGTGGTGTTTATAGAGGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTCGTGCTGAGAGAGATGGCCAAGGCCTTGGGGTCCACAAGGCTGAGTCGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

139

Amino Acids

15.61

Weight (kDa)

5.75

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 41 - 118 1.4e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 301
AccI GTMKAC 1 cut(s) 344
AccII CGCG 1 cut(s) 212
AciI CCGC 6 cut(s) 35, 96, 150, 164, 241, 290
AclWI GGATC 1 cut(s) 69
AcoI YGGCCR 2 cut(s) 165, 381
AcyI GRCGYC 2 cut(s) 203, 341
AfaI GTAC 1 cut(s) 272
AjiI CACGTC 3 cut(s) 31, 85, 235
AluBI AGCT 2 cut(s) 286, 331
AluI AGCT 2 cut(s) 286, 331
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 1 cut(s) 69
AoxI GGCC 5 cut(s) 165, 179, 245, 381, 387
ApeKI GCWGC 3 cut(s) 114, 286, 331
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 396
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 396
BalI TGGCCA 1 cut(s) 383
BanI GGYRCC 1 cut(s) 301
BbsI GAAGAC 2 cut(s) 314, 344
BbvI GCAGC 3 cut(s) 126, 273, 318
BccI CCATC 1 cut(s) 373
BceAI ACGGC 3 cut(s) 137, 152, 190
BclI TGATCA 1 cut(s) 87
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 274
BglI GCCNNNNNGGC 1 cut(s) 173
BisI GCNGC 5 cut(s) 115, 165, 287, 290, 332
BlsI GCNGC 5 cut(s) 116, 166, 288, 291, 333
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 396
BmgBI CACGTC 3 cut(s) 31, 85, 235
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 2 cut(s) 303, 397
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 30
BpiI GAAGAC 2 cut(s) 314, 344
BpuEI CTTGAG 2 cut(s) 80, 130
BpuMI CCSGG 1 cut(s) 47
BsaHI GRCGYC 2 cut(s) 203, 341
BsaJI CCNNGG 4 cut(s) 78, 168, 384, 390
BsaXI ACNNNNNCTCC 6 cut(s) 113, 131, 143, 161, 191, 221
Bse118I RCCGGY 1 cut(s) 23
BseDI CCNNGG 4 cut(s) 78, 168, 384, 390
BseGI GGATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 362, 398
BseRI GAGGAG 1 cut(s) 341
BseX3I CGGCCG 1 cut(s) 165
BseXI GCAGC 3 cut(s) 126, 273, 318
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 168
BshFI GGCC 5 cut(s) 167, 181, 247, 383, 389
BshNI GGYRCC 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 168
BsiSI CCGG 2 cut(s) 24, 47
BsmAI GTCTC 1 cut(s) 194
BsmBI CGTCTC 1 cut(s) 194
BsnI GGCC 5 cut(s) 167, 181, 247, 383, 389
Bsp143I GATC 3 cut(s) 63, 74, 87
BspACI CCGC 6 cut(s) 35, 96, 150, 164, 241, 290
BspANI GGCC 5 cut(s) 167, 181, 247, 383, 389
BspCNI CTCAG 2 cut(s) 363, 399
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 303, 397
BspMAI CTGCAG 1 cut(s) 278
BspPI GGATC 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 4 cut(s) 78, 168, 384, 390
BssMI GATC 3 cut(s) 63, 74, 87
BssNI GRCGYC 2 cut(s) 203, 341
BssT1I CCWWGG 3 cut(s) 78, 384, 390
Bst4CI ACNGT 2 cut(s) 11, 313
BstACI GRCGYC 2 cut(s) 203, 341
BstDEI CTNAG 2 cut(s) 371, 407
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 420
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 66, 77, 90
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 63, 74, 87
BstMCI CGRYCG 1 cut(s) 168
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 274
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 3 cut(s) 126, 273, 318
BstV2I GAAGAC 2 cut(s) 314, 344
BstX2I RGATCY 1 cut(s) 74
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 1 cut(s) 74
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 5 cut(s) 167, 181, 247, 383, 389
BtgI CCRYGG 1 cut(s) 168
BtrI CACGTC 3 cut(s) 31, 85, 235
BtsCI GGATG 1 cut(s) 420
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 396
CseI GACGC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 271
CviAII CATG 1 cut(s) 298
CviJI RGCY 9 cut(s) 167, 181, 222, 247, 286, 331, 383, 389, 406
CviKI_1 RGCY 9 cut(s) 167, 181, 222, 247, 286, 331, 383, 389, 406
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 2 cut(s) 371, 407
DpnI GATC 3 cut(s) 65, 76, 89
DpnII GATC 3 cut(s) 63, 74, 87
EaeI YGGCCR 2 cut(s) 165, 381
EagI CGGCCG 1 cut(s) 165
EciI GGCGGA 2 cut(s) 139, 256
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 3 cut(s) 78, 384, 390
Eco147I AGGCCT 2 cut(s) 181, 389
Eco47I GGWCC 1 cut(s) 396
Eco52I CGGCCG 1 cut(s) 165
EcoT14I CCWWGG 3 cut(s) 78, 384, 390
ErhI CCWWGG 3 cut(s) 78, 384, 390
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 1 cut(s) 301
FaiI YATR 3 cut(s) 92, 299, 323
FatI CATG 1 cut(s) 297
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 87
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 5 cut(s) 115, 165, 287, 290, 332
Fsp4HI GCNGC 5 cut(s) 115, 165, 287, 290, 332
GlaI GCGC 1 cut(s) 211
GluI GCNGC 5 cut(s) 115, 165, 287, 290, 332
HaeIII GGCC 5 cut(s) 167, 181, 247, 383, 389
HapII CCGG 2 cut(s) 24, 47
HgaI GACGC 1 cut(s) 211
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 2 cut(s) 203, 341
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HinfI GANTC 3 cut(s) 128, 355, 409
HpaII CCGG 2 cut(s) 24, 47
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 3 cut(s) 157, 345, 399
Hpy188I TCNGA 2 cut(s) 360, 414
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 3 cut(s) 157, 345, 399
Hpy99I CGWCG 4 cut(s) 32, 253, 346, 349
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 2 cut(s) 11, 313
HpyCH4IV ACGT 4 cut(s) 30, 84, 234, 341
HpyCH4V TGCA 2 cut(s) 43, 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 371, 407
HpySE526I ACGT 4 cut(s) 30, 84, 234, 341
Hsp92I GRCGYC 2 cut(s) 203, 341
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 87
Kzo9I GATC 3 cut(s) 63, 74, 87
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 5 cut(s) 37, 60, 178, 195, 236
Lsp1109I GCAGC 3 cut(s) 126, 273, 318
LweI GCATC 1 cut(s) 30
MaeII ACGT 4 cut(s) 30, 84, 234, 341
MalI GATC 3 cut(s) 65, 76, 89
MboI GATC 3 cut(s) 63, 74, 87
MboII GAAGA 3 cut(s) 319, 349, 364
MflI RGATCY 1 cut(s) 74
MlsI TGGCCA 1 cut(s) 383
MluNI TGGCCA 1 cut(s) 383
MlyI GAGTC 2 cut(s) 137, 418
MmeI TCCRAC 1 cut(s) 392
MnlI CCTC 6 cut(s) 115, 154, 171, 237, 272, 319
Mox20I TGGCCA 1 cut(s) 383
MscI TGGCCA 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 383
MspI CCGG 2 cut(s) 24, 47
MspR9I CCNGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 173
NciI CCSGG 1 cut(s) 47
NdeII GATC 3 cut(s) 63, 74, 87
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 2 cut(s) 303, 397
PceI AGGCCT 2 cut(s) 181, 389
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 209
PfeI GAWTC 1 cut(s) 355
PkrI GCNGC 5 cut(s) 116, 166, 288, 291, 333
PleI GAGTC 2 cut(s) 136, 417
PpsI GAGTC 2 cut(s) 136, 417
PspN4I GGNNCC 2 cut(s) 303, 397
PspPI GGNCC 1 cut(s) 396
PstI CTGCAG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
SalI GTCGAC 1 cut(s) 343
SatI GCNGC 5 cut(s) 115, 165, 287, 290, 332
Sau3AI GATC 3 cut(s) 63, 74, 87
Sau96I GGNCC 1 cut(s) 396
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 2 cut(s) 137, 418
ScrFI CCNGG 1 cut(s) 47
SetI ASST 7 cut(s) 33, 87, 126, 237, 288, 333, 344
SfaNI GCATC 1 cut(s) 30
SfcI CTRYAG 1 cut(s) 274
SgrAI CRCCGGYG 1 cut(s) 23
SgrDI CGTCGACG 1 cut(s) 343
SinI GGWCC 1 cut(s) 396
SmlI CTYRAG 2 cut(s) 59, 109
SmoI CTYRAG 2 cut(s) 59, 109
SseBI AGGCCT 2 cut(s) 181, 389
SsiI CCGC 6 cut(s) 35, 96, 150, 164, 241, 290
StuI AGGCCT 2 cut(s) 181, 389
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 3 cut(s) 78, 384, 390
TaaI ACNGT 2 cut(s) 11, 313
TaiI ACGT 4 cut(s) 33, 87, 237, 344
TaqI TCGA 1 cut(s) 344
TatI WGTACW 1 cut(s) 270
TauI GCSGC 2 cut(s) 167, 292
TfiI GAWTC 1 cut(s) 355
TseI GCWGC 3 cut(s) 114, 286, 331
TspDTI ATGAA 2 cut(s) 17, 286
TspGWI ACGGA 1 cut(s) 266
VpaK11BI GGWCC 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 344
ZraI GACGTC 1 cut(s) 342
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.