RLG00000023000

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000677
Physical Location & Seq
Forward (+)
16468171 .. 16469838
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023000

Sequence Viewer

Length: 1668 bp
ATGGAGGAGCTGAAGCCAACCCCCGCCAACTCATCCCCGCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGACCACACCACCTACACGTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCATCCGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCTCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGACGCCCTCCGTCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACCATGATGCACCCGCAGCCGCACCCCATCCCAACTCCGACTCATCCCCGTTCTGCGCCACATACGAGGAAATGGTGGAGAGCGGCGATCCGGACTTCCAGTGGGCCCTACCGGAGAACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACGACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACGCCAACGGATGGAGCTACCCTTACGGGATCGCCGCCGTGGGAGGGACCAACGTCTGCGTCCGCAAATTCGACGCGCATGTCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACGAATGTACCGAACAACGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCGCCCGCGGCGGTTCTGTCTCGGACTGAGTCGCTGGGATTCGTGGTGAGCCATGGGTACGGGTTGACGGAGACGGCGGGGCTGGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCGGCCACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGACTGAGGACGGTGGCCATGACCCGGGTGGACGTGGTGGATCCTGATACGGGTAAGCCGGTTAAACGCGACGGTTCGTCGCTGGGGGAGGTGGTTTTCAGAGGCGGGTGTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCTGTGATGCACCCGGACGGGTATCTGGAGATCAAGGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACTCCGCCGTGAACGAGGCGGCCGTGGTGGCGAGGCCCGACGAGTTCTGGGGGGAGACACCCTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGAGGAGGAGGCCGACGGAGAAGGAGATAATAGAGTATTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCATAGAGGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTCGTGCTGAGAGAGATGGCCAAGGCCTTGGGGTCCACAAGGCTGAGTCGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

556

Amino Acids

60.95

Weight (kDa)

7.61

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 406 2e-76 AMP-binding enzyme
AMP-binding_C PF13193 456 - 534 1.3e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 794
AatII GACGTC 1 cut(s) 1592
AccB1I GGYRCC 2 cut(s) 263, 1549
AccB7I CCANNNNNTGG 1 cut(s) 726
AccBSI CCGCTC 2 cut(s) 40, 519
AccI GTMKAC 3 cut(s) 78, 351, 1592
AccII CGCG 7 cut(s) 153, 189, 791, 938, 1028, 1158, 1457
AccIII TCCGGA 1 cut(s) 526
AclWI GGATC 5 cut(s) 518, 752, 1124, 1137, 1314
AcoI YGGCCR 6 cut(s) 190, 1056, 1104, 1236, 1410, 1629
AcsI RAATTY 2 cut(s) 782, 1540
AcuI CTGAAG 2 cut(s) 32, 1097
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 4 cut(s) 264, 381, 825, 1589
AfaI GTAC 4 cut(s) 233, 675, 876, 989
AfiI CCNNNNNNNGG 8 cut(s) 123, 260, 667, 726, 759, 1065, 1113, 1139
AflIII ACRYGT 2 cut(s) 120, 834
AhdI GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
AjiI CACGTC 5 cut(s) 196, 1123, 1276, 1330, 1480
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 569, 601
AluBI AGCT 8 cut(s) 10, 238, 340, 366, 732, 926, 1534, 1579
AluI AGCT 8 cut(s) 10, 238, 340, 366, 732, 926, 1534, 1579
Alw21I GWGCWC 2 cut(s) 240, 928
Alw26I GTCTC 6 cut(s) 205, 313, 395, 954, 995, 1439
AlwI GGATC 5 cut(s) 518, 752, 1124, 1137, 1314
AlwNI CAGNNNCTG 1 cut(s) 1093
Ama87I CYCGRG 2 cut(s) 1037, 1113
Aor13HI TCCGGA 1 cut(s) 526
ApaI GGGCCC 1 cut(s) 544
ApeKI GCWGC 4 cut(s) 452, 1359, 1534, 1579
ApoI RAATTY 2 cut(s) 782, 1540
AspLEI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1457
AsuC2I CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
AsuHPI GGTGA 3 cut(s) 34, 637, 988
AvaI CYCGRG 2 cut(s) 1037, 1113
AvaII GGWCC 6 cut(s) 562, 616, 762, 853, 904, 1644
BaeGI GKGCMC 1 cut(s) 544
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BalI TGGCCA 2 cut(s) 1106, 1631
BamHI GGATCC 1 cut(s) 1129
BanI GGYRCC 2 cut(s) 263, 1549
BanII GRGCYC 4 cut(s) 240, 544, 636, 928
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 1562, 1592
Bbv12I GWGCWC 2 cut(s) 240, 928
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 4 cut(s) 464, 1371, 1521, 1566
BccI CCATC 5 cut(s) 173, 287, 471, 720, 1621
BceAI ACGGC 9 cut(s) 97, 251, 656, 737, 838, 1020, 1251, 1382, 1397
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BclI TGATCA 2 cut(s) 810, 1332
BcnI CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
BcoDI GTCTC 6 cut(s) 205, 313, 395, 954, 995, 1439
BfaI CTAG 1 cut(s) 59
BfoI RGCGCY 1 cut(s) 267
BglI GCCNNNNNGGC 1 cut(s) 1418
Bme1390I CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
Bme18I GGWCC 6 cut(s) 562, 616, 762, 853, 904, 1644
BmeRI GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
BmeT110I CYCGRG 2 cut(s) 1037, 1113
BmgBI CACGTC 5 cut(s) 196, 1123, 1276, 1330, 1480
BmrFI CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
BmrI ACTGGG 1 cut(s) 676
BmsI GCATC 4 cut(s) 134, 189, 433, 1275
BmuI ACTGGG 1 cut(s) 676
BoxI GACNNNNGTC 1 cut(s) 767
BpiI GAAGAC 2 cut(s) 1562, 1592
BpmI CTGGAG 1 cut(s) 1325
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 1375
BpuMI CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 4 cut(s) 264, 381, 825, 1589
BsaWI WCCGGW 2 cut(s) 526, 547
BsaXI ACNNNNNCTCC 8 cut(s) 185, 215, 542, 572, 1358, 1376, 1388, 1406
Bsc4I CCNNNNNNNGG 8 cut(s) 123, 260, 667, 726, 759, 1065, 1113, 1139
Bse118I RCCGGY 4 cut(s) 847, 1054, 1147, 1268
Bse1I ACTGG 3 cut(s) 535, 671, 1239
BseAI TCCGGA 1 cut(s) 526
BseGI GGATG 7 cut(s) 32, 180, 219, 463, 479, 731, 1668
BseLI CCNNNNNNNGG 8 cut(s) 123, 260, 667, 726, 759, 1065, 1113, 1139
BseMII CTCAG 5 cut(s) 376, 948, 1085, 1610, 1646
BseNI ACTGG 3 cut(s) 535, 671, 1239
BseSI GKGCMC 1 cut(s) 544
BseX3I CGGCCG 1 cut(s) 1410
BseXI GCAGC 4 cut(s) 464, 1371, 1521, 1566
BseYI CCCAGC 2 cut(s) 964, 1171
Bsh1236I CGCG 7 cut(s) 153, 189, 791, 938, 1028, 1158, 1457
Bsh1285I CGRYCG 1 cut(s) 1413
BshNI GGYRCC 2 cut(s) 263, 1549
BsiEI CGRYCG 1 cut(s) 1413
BsiHKAI GWGCWC 2 cut(s) 240, 928
BsiHKCI CYCGRG 2 cut(s) 1037, 1113
BslFI GGGAC 4 cut(s) 236, 575, 775, 1104
BslI CCNNNNNNNGG 8 cut(s) 123, 260, 667, 726, 759, 1065, 1113, 1139
BsmAI GTCTC 6 cut(s) 205, 313, 395, 954, 995, 1439
BsmBI CGTCTC 4 cut(s) 205, 313, 395, 995
BsmFI GGGAC 4 cut(s) 236, 575, 775, 1104
BsoBI CYCGRG 2 cut(s) 1037, 1113
Bsp120I GGGCCC 1 cut(s) 540
Bsp1286I GDGCHC 4 cut(s) 240, 544, 636, 928
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
Bsp19I CCATGG 1 cut(s) 982
BspCNI CTCAG 5 cut(s) 375, 949, 1086, 1611, 1647
BspEI TCCGGA 1 cut(s) 526
BspFNI CGCG 7 cut(s) 153, 189, 791, 938, 1028, 1158, 1457
BspHI TCATGA 1 cut(s) 912
BspPI GGATC 5 cut(s) 518, 752, 1124, 1137, 1314
BspQI GCTCTTC 1 cut(s) 641
BspT107I GGYRCC 2 cut(s) 263, 1549
BsrBI CCGCTC 2 cut(s) 40, 519
BsrFI RCCGGY 4 cut(s) 847, 1054, 1147, 1268
BsrI ACTGG 3 cut(s) 535, 671, 1239
BssAI RCCGGY 4 cut(s) 847, 1054, 1147, 1268
BssMI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
BssNI GRCGYC 4 cut(s) 264, 381, 825, 1589
BssT1I CCWWGG 5 cut(s) 606, 982, 1323, 1632, 1638
Bst4CI ACNGT 8 cut(s) 76, 140, 307, 649, 1102, 1163, 1256, 1561
Bst6I CTCTTC 3 cut(s) 350, 398, 641
BstACI GRCGYC 4 cut(s) 264, 381, 825, 1589
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 936, 1056
BstDEI CTNAG 5 cut(s) 362, 957, 1094, 1619, 1655
BstDSI CCRYGG 5 cut(s) 753, 936, 982, 1059, 1413
BstF5I GGATG 7 cut(s) 32, 180, 219, 463, 479, 731, 1668
BstFNI CGCG 7 cut(s) 153, 189, 791, 938, 1028, 1158, 1457
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1457
BstKTI GATC 8 cut(s) 526, 747, 813, 912, 1132, 1311, 1322, 1335
BstMAI GTCTC 6 cut(s) 205, 313, 395, 954, 995, 1439
BstMBI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
BstMCI CGRYCG 1 cut(s) 1413
BstMWI GCNNNNNNNGC 3 cut(s) 186, 452, 1418
BstNSI RCATGY 3 cut(s) 797, 838, 865
BstPAI GACNNNNGTC 1 cut(s) 767
BstSCI CCNGG 6 cut(s) 900, 1036, 1037, 1112, 1113, 1290
BstSLI GKGCMC 1 cut(s) 544
BstUI CGCG 7 cut(s) 153, 189, 791, 938, 1028, 1158, 1457
BstV1I GCAGC 4 cut(s) 464, 1371, 1521, 1566
BstV2I GAAGAC 2 cut(s) 1562, 1592
BstX2I RGATCY 2 cut(s) 1129, 1319
BstXI CCANNNNNNTGG 2 cut(s) 613, 1639
BstYI RGATCY 2 cut(s) 1129, 1319
BstZI CGGCCG 1 cut(s) 1410
BtgI CCRYGG 5 cut(s) 753, 936, 982, 1059, 1413
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 5 cut(s) 196, 1123, 1276, 1330, 1480
BtsCI GGATG 7 cut(s) 32, 180, 219, 463, 479, 731, 1668
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 542, 619
Cac8I GCNNGC 2 cut(s) 936, 1056
CaiI CAGNNNCTG 1 cut(s) 1093
CciI TCATGA 1 cut(s) 912
CfoI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1457
Cfr10I RCCGGY 4 cut(s) 847, 1054, 1147, 1268
Cfr42I CCGCGG 1 cut(s) 939
Cfr9I CCCGGG 2 cut(s) 1037, 1113
CseI GACGC 8 cut(s) 142, 195, 389, 580, 709, 763, 797, 814
Csp6I GTAC 4 cut(s) 232, 674, 875, 988
CviQI GTAC 4 cut(s) 232, 674, 875, 988
DdeI CTNAG 5 cut(s) 362, 957, 1094, 1619, 1655
DinI GGCGCC 1 cut(s) 265
DpnI GATC 8 cut(s) 525, 746, 812, 911, 1131, 1310, 1321, 1334
DpnII GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
DrdI GACNNNNNNGTC 1 cut(s) 794
DriI GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
DseDI GACNNNNNNGTC 1 cut(s) 794
EaeI YGGCCR 6 cut(s) 190, 1056, 1104, 1236, 1410, 1629
EagI CGGCCG 1 cut(s) 1410
Eam1104I CTCTTC 3 cut(s) 350, 398, 641
Eam1105I GACNNNNNGTC 4 cut(s) 138, 686, 824, 1165
EarI CTCTTC 3 cut(s) 350, 398, 641
EciI GGCGGA 3 cut(s) 311, 918, 1384
Ecl136II GAGCTC 2 cut(s) 238, 926
EclXI CGGCCG 1 cut(s) 1410
Eco130I CCWWGG 5 cut(s) 606, 982, 1323, 1632, 1638
Eco147I AGGCCT 1 cut(s) 1637
Eco24I GRGCYC 4 cut(s) 240, 544, 636, 928
Eco47I GGWCC 6 cut(s) 562, 616, 762, 853, 904, 1644
Eco52I CGGCCG 1 cut(s) 1410
Eco53kI GAGCTC 2 cut(s) 238, 926
Eco57I CTGAAG 2 cut(s) 32, 1097
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 2 cut(s) 1037, 1113
EcoICRI GAGCTC 2 cut(s) 238, 926
EcoO109I RGGNCCY 2 cut(s) 541, 562
EcoT14I CCWWGG 5 cut(s) 606, 982, 1323, 1632, 1638
EcoT38I GRGCYC 4 cut(s) 240, 544, 636, 928
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 5 cut(s) 606, 982, 1323, 1632, 1638
Esp3I CGTCTC 4 cut(s) 205, 313, 395, 995
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 4 cut(s) 236, 575, 775, 1104
FbaI TGATCA 2 cut(s) 810, 1332
FblI GTMKAC 3 cut(s) 78, 351, 1592
FokI GGATG 6 cut(s) 19, 167, 206, 450, 466, 738
FriOI GRGCYC 4 cut(s) 240, 544, 636, 928
FspBI CTAG 1 cut(s) 59
GlaI GCGC 6 cut(s) 265, 493, 792, 846, 1027, 1456
GsaI CCCAGC 2 cut(s) 968, 1175
GsuI CTGGAG 1 cut(s) 1325
HaeII RGCGCY 1 cut(s) 267
HgaI GACGC 8 cut(s) 142, 195, 389, 580, 709, 763, 797, 814
HhaI GCGC 6 cut(s) 266, 494, 793, 847, 1028, 1457
Hin1I GRCGYC 4 cut(s) 264, 381, 825, 1589
Hin6I GCGC 6 cut(s) 264, 492, 791, 845, 1026, 1455
HinP1I GCGC 6 cut(s) 264, 492, 791, 845, 1026, 1455
HincII GTYRAC 4 cut(s) 352, 652, 996, 1593
HindII GTYRAC 4 cut(s) 352, 652, 996, 1593
HinfI GANTC 6 cut(s) 476, 959, 969, 1373, 1603, 1657
HphI GGTGA 3 cut(s) 34, 637, 988
Hpy166II GTNNAC 9 cut(s) 79, 352, 619, 652, 996, 1120, 1402, 1593, 1647
Hpy188I TCNGA 9 cut(s) 185, 475, 708, 815, 954, 1190, 1218, 1608, 1662
Hpy188III TCNNGA 6 cut(s) 527, 697, 907, 913, 1133, 1304
Hpy8I GTNNAC 9 cut(s) 79, 352, 619, 652, 996, 1120, 1402, 1593, 1647
HpyAV CCTTC 2 cut(s) 102, 1498
HpyCH4III ACNGT 8 cut(s) 76, 140, 307, 649, 1102, 1163, 1256, 1561
HpyCH4IV ACGT 8 cut(s) 122, 195, 768, 1122, 1275, 1329, 1479, 1589
HpyCH4V TGCA 4 cut(s) 446, 1245, 1288, 1524
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 452, 1418
HpyF3I CTNAG 5 cut(s) 362, 957, 1094, 1619, 1655
HpySE526I ACGT 8 cut(s) 122, 195, 768, 1122, 1275, 1329, 1479, 1589
Hsp92I GRCGYC 4 cut(s) 264, 381, 825, 1589
HspAI GCGC 6 cut(s) 264, 492, 791, 845, 1026, 1455
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 562
Kpn2I TCCGGA 1 cut(s) 526
KroI GCCGGC 1 cut(s) 1054
KroNI GCCGGC 1 cut(s) 1056
Ksp22I TGATCA 2 cut(s) 810, 1332
KspI CCGCGG 1 cut(s) 939
Kzo9I GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 9 cut(s) 7, 126, 243, 345, 371, 729, 923, 931, 1576
Lsp1109I GCAGC 4 cut(s) 464, 1371, 1521, 1566
LweI GCATC 4 cut(s) 134, 189, 433, 1275
MaeI CTAG 1 cut(s) 59
MaeII ACGT 8 cut(s) 122, 195, 768, 1122, 1275, 1329, 1479, 1589
MaeIII GTNAC 1 cut(s) 826
MalI GATC 8 cut(s) 525, 746, 812, 911, 1131, 1310, 1321, 1334
MbiI CCGCTC 2 cut(s) 40, 519
MboI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
MboII GAAGA 6 cut(s) 337, 385, 628, 1567, 1597, 1612
MflI RGATCY 2 cut(s) 1129, 1319
MhlI GDGCHC 4 cut(s) 240, 544, 636, 928
MlsI TGGCCA 2 cut(s) 1106, 1631
MluCI AATT 2 cut(s) 782, 1540
MluNI TGGCCA 2 cut(s) 1106, 1631
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 4 cut(s) 470, 968, 1382, 1666
MmeI TCCRAC 3 cut(s) 208, 498, 1640
Mox20I TGGCCA 2 cut(s) 1106, 1631
MroI TCCGGA 1 cut(s) 526
MroNI GCCGGC 1 cut(s) 1054
MscI TGGCCA 2 cut(s) 1106, 1631
MseI TTAA 1 cut(s) 1152
MslI CAYNNNNRTG 1 cut(s) 833
Msp20I TGGCCA 2 cut(s) 1106, 1631
MspA1I CMGCKG 1 cut(s) 938
MspR9I CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
MvnI CGCG 7 cut(s) 153, 189, 791, 938, 1028, 1158, 1457
MwoI GCNNNNNNNGC 3 cut(s) 186, 452, 1418
NaeI GCCGGC 1 cut(s) 1056
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
NcoI CCATGG 1 cut(s) 982
NdeII GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
NgoMIV GCCGGC 1 cut(s) 1054
NmeAIII GCCGAG 2 cut(s) 30, 156
NmuCI GTSAC 1 cut(s) 826
NspI RCATGY 3 cut(s) 797, 838, 865
PagI TCATGA 1 cut(s) 912
PceI AGGCCT 1 cut(s) 1637
PciI ACATGT 1 cut(s) 834
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 5 cut(s) 201, 669, 875, 1067, 1410
PdiI GCCGGC 1 cut(s) 1056
PfeI GAWTC 2 cut(s) 969, 1603
PflFI GACNNNGTC 1 cut(s) 958
PflMI CCANNNNNTGG 1 cut(s) 726
PleI GAGTC 4 cut(s) 470, 967, 1381, 1665
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 4 cut(s) 470, 967, 1381, 1665
Ppu21I YACGTR 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 562
PscI ACATGT 1 cut(s) 834
PshAI GACNNNNGTC 1 cut(s) 767
Psp124BI GAGCTC 2 cut(s) 240, 928
Psp5II RGGWCCY 1 cut(s) 562
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 2 cut(s) 964, 1171
PspOMI GGGCCC 1 cut(s) 540
PspPPI RGGWCCY 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 1093
PsuI RGATCY 2 cut(s) 1129, 1319
PsyI GACNNNGTC 1 cut(s) 958
RsaI GTAC 4 cut(s) 233, 675, 876, 989
RsaNI GTAC 4 cut(s) 232, 674, 875, 988
RseI CAYNNNNRTG 1 cut(s) 833
SacI GAGCTC 2 cut(s) 240, 928
SacII CCGCGG 1 cut(s) 939
SalI GTCGAC 2 cut(s) 350, 1591
SapI GCTCTTC 1 cut(s) 641
SaqAI TTAA 1 cut(s) 1152
Sau3AI GATC 8 cut(s) 523, 744, 810, 909, 1129, 1308, 1319, 1332
SchI GAGTC 4 cut(s) 470, 968, 1382, 1666
ScrFI CCNGG 6 cut(s) 902, 1038, 1039, 1114, 1115, 1292
SduI GDGCHC 4 cut(s) 240, 544, 636, 928
SfaNI GCATC 4 cut(s) 134, 189, 433, 1275
SfoI GGCGCC 1 cut(s) 265
Sfr303I CCGCGG 1 cut(s) 939
SgrAI CRCCGGYG 2 cut(s) 847, 1268
SgrBI CCGCGG 1 cut(s) 939
SgrDI CGTCGACG 1 cut(s) 1591
SinI GGWCC 6 cut(s) 562, 616, 762, 853, 904, 1644
SmaI CCCGGG 2 cut(s) 1039, 1115
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 1354
SmoI CTYRAG 1 cut(s) 1354
Sse9I AATT 2 cut(s) 782, 1540
SseBI AGGCCT 1 cut(s) 1637
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 2 cut(s) 240, 928
StuI AGGCCT 1 cut(s) 1637
StyD4I CCNGG 6 cut(s) 900, 1036, 1037, 1112, 1113, 1290
StyI CCWWGG 5 cut(s) 606, 982, 1323, 1632, 1638
TaaI ACNGT 8 cut(s) 76, 140, 307, 649, 1102, 1163, 1256, 1561
TaiI ACGT 8 cut(s) 125, 198, 771, 1125, 1278, 1332, 1482, 1592
TaqI TCGA 8 cut(s) 142, 294, 351, 378, 663, 786, 1082, 1592
TaqII GACCGA 1 cut(s) 78
TasI AATT 2 cut(s) 782, 1540
TauI GCSGC 8 cut(s) 71, 192, 458, 522, 752, 934, 941, 1412
TfiI GAWTC 2 cut(s) 969, 1603
Tru1I TTAA 1 cut(s) 1152
Tru9I TTAA 1 cut(s) 1152
TscAI CASTG 2 cut(s) 542, 626
TseFI GTSAC 1 cut(s) 826
TseI GCWGC 4 cut(s) 452, 1359, 1534, 1579
Tsp45I GTSAC 1 cut(s) 826
TspDTI ATGAA 4 cut(s) 628, 1262, 1534, 1558
TspGWI ACGGA 7 cut(s) 193, 238, 377, 738, 1013, 1076, 1514
TspMI CCCGGG 2 cut(s) 1037, 1113
TspRI CASTG 2 cut(s) 542, 626
Tth111I GACNNNGTC 1 cut(s) 958
Van91I CCANNNNNTGG 1 cut(s) 726
VpaK11BI GGWCC 6 cut(s) 562, 616, 762, 853, 904, 1644
XapI RAATTY 2 cut(s) 782, 1540
XceI RCATGY 3 cut(s) 797, 838, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 1114
XmaI CCCGGG 2 cut(s) 1037, 1113
XmiI GTMKAC 3 cut(s) 78, 351, 1592
XspI CTAG 1 cut(s) 59
ZraI GACGTC 1 cut(s) 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.