pycom09g19100

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000677
Physical Location & Seq
Forward (+)
19890373 .. 19891272
900 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g19100.3

Sequence Viewer

Length: 816 bp
ATGGAGCAGCTCAAACCAAACCCTGCAAATCTCTGCCCTCTCACTCCTCTCACCCTGCTAGAAAGGGCAGCCATCGTCTACGGCAATTGCCCCTCCATCGTCTACACCACCACCACCTACACGTGGTCCCAAACACACCGCCGATGCCTCCAGCTGGCCTCCTCCCTCTCATCCCACCTCGGCATCAAAAAATCCCAAGTCGTCTCTGTATTCTCCCCCAACACCCTTGCCATGTACGAGCTCCACTTCGCCGTCCCCATGGCAGGAGCCATCCTCAACACCATCAACACCCGCCTCGACGCCCGCACCGTCTCCGTCATCCTCCGCCACAGCGAATCAAAACTCATCTTCGTCGACCACCTCTTTGCCTCTCTCGTACTGGAAGCTCTCTCGCTCTTCCCACCTCAGGACGGATCAATCCCAACCGTCGATCATTTCATCGACACTTACGAGAGTCTAGTGGGAAAGGGGGATCCGCTGTTTAAGTGGGTCCGGCCGGTTTCCGAGTGGGACCCAATCGTTTTAAACTACACGTCCGGAACGACGTCGGCTCCGAAGGGGGTTGTGCATTGCCACAGAGCCGTTTACAACGTGGCCCTGAGTTCCCTCACGGACTGGTCCGTGCCCAAAAACTGCGTCTACCTGTGGACTCTACCGATGTTCCACGCAAACGGGTGGTGCTTCCCGTGGGCCATGGCCGCCGTCGGCGGGACCAACATTTGCGTCCGCAAATTCGATGGCCCAAAAATCTACGAGTTAATCGAAGCTAACGGCGTCACCCACATGTGCGCCGCGCCCGTCGTGCTCAACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

272

Amino Acids

29.93

Weight (kDa)

7.63

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 548
AccI GTMKAC 4 cut(s) 78, 102, 354, 639
AccII CGCG 1 cut(s) 794
AccIII TCCGGA 1 cut(s) 536
AciI CCGC 9 cut(s) 139, 292, 304, 325, 476, 699, 708, 727, 792
AclWI GGATC 3 cut(s) 421, 467, 480
AcoI YGGCCR 2 cut(s) 494, 696
AcsI RAATTY 1 cut(s) 731
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 3 cut(s) 300, 545, 774
AfaI GTAC 2 cut(s) 236, 378
AfiI CCNNNNNNNGG 6 cut(s) 123, 154, 263, 406, 410, 708
AflIII ACRYGT 3 cut(s) 120, 531, 783
AjiI CACGTC 1 cut(s) 534
AluBI AGCT 5 cut(s) 10, 154, 241, 386, 767
AluI AGCT 5 cut(s) 10, 154, 241, 386, 767
Alw21I GWGCWC 2 cut(s) 243, 807
Alw26I GTCTC 2 cut(s) 208, 316
AlwI GGATC 3 cut(s) 421, 467, 480
Aor13HI TCCGGA 1 cut(s) 536
AoxI GGCC 6 cut(s) 156, 494, 594, 690, 696, 739
ApeKI GCWGC 2 cut(s) 7, 68
ApoI RAATTY 1 cut(s) 731
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 621, 653
AspLEI GCGC 2 cut(s) 791, 796
AspS9I GGNCC 8 cut(s) 126, 490, 511, 595, 618, 690, 711, 740
AsuHPI GGTGA 2 cut(s) 43, 769
AvaII GGWCC 5 cut(s) 126, 490, 511, 618, 711
AxyI CCTNAGG 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 627
BamHI GGATCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 243
BbrPI CACGTG 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 243, 807
BbvI GCAGC 2 cut(s) 19, 80
BccI CCATC 5 cut(s) 80, 104, 278, 290, 731
BceAI ACGGC 5 cut(s) 97, 236, 566, 686, 787
BcoDI GTCTC 2 cut(s) 208, 316
BfaI CTAG 2 cut(s) 59, 458
BisI GCNGC 4 cut(s) 8, 69, 699, 792
BlsI GCNGC 4 cut(s) 9, 70, 700, 793
Bme18I GGWCC 5 cut(s) 126, 490, 511, 618, 711
BmgBI CACGTC 1 cut(s) 534
BmgT120I GGNCC 8 cut(s) 126, 490, 511, 595, 618, 690, 711, 740
BmiI GGNNCC 8 cut(s) 128, 268, 474, 491, 512, 513, 552, 712
BmsI GCATC 2 cut(s) 134, 192
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 3 cut(s) 300, 545, 774
BsaJI CCNNGG 4 cut(s) 178, 258, 686, 693
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 154, 263, 406, 410, 708
Bse118I RCCGGY 1 cut(s) 496
Bse1I ACTGG 2 cut(s) 384, 620
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 568
BseAI TCCGGA 1 cut(s) 536
BseDI CCNNGG 4 cut(s) 178, 258, 686, 693
BseGI GGATG 3 cut(s) 170, 270, 318
BseLI CCNNNNNNNGG 6 cut(s) 123, 154, 263, 406, 410, 708
BseMI GCAATG 1 cut(s) 568
BseMII CTCAG 2 cut(s) 419, 590
BseNI ACTGG 2 cut(s) 384, 620
BseRI GAGGAG 2 cut(s) 36, 151
BseSI GKGCMC 1 cut(s) 627
BseX3I CGGCCG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 19, 80
Bsh1236I CGCG 1 cut(s) 794
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 6 cut(s) 158, 496, 596, 692, 698, 741
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 2 cut(s) 243, 807
BsiSI CCGG 3 cut(s) 493, 497, 537
BslFI GGGAC 4 cut(s) 112, 239, 524, 724
BslI CCNNNNNNNGG 6 cut(s) 123, 154, 263, 406, 410, 708
BsmAI GTCTC 2 cut(s) 208, 316
BsmBI CGTCTC 2 cut(s) 208, 316
BsmFI GGGAC 4 cut(s) 112, 239, 524, 724
BsnI GGCC 6 cut(s) 158, 496, 596, 692, 698, 741
Bsp1286I GDGCHC 3 cut(s) 243, 627, 807
Bsp13I TCCGGA 1 cut(s) 536
Bsp143I GATC 3 cut(s) 413, 430, 472
Bsp19I CCATGG 2 cut(s) 258, 693
BspACI CCGC 9 cut(s) 139, 292, 304, 325, 476, 699, 708, 727, 792
BspANI GGCC 6 cut(s) 158, 496, 596, 692, 698, 741
BspCNI CTCAG 2 cut(s) 418, 591
BspEI TCCGGA 1 cut(s) 536
BspFNI CGCG 1 cut(s) 794
BspLI GGNNCC 8 cut(s) 128, 268, 474, 491, 512, 513, 552, 712
BspPI GGATC 3 cut(s) 421, 467, 480
BspQI GCTCTTC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 568
BsrFI RCCGGY 1 cut(s) 496
BsrI ACTGG 2 cut(s) 384, 620
BssAI RCCGGY 1 cut(s) 496
BssECI CCNNGG 4 cut(s) 178, 258, 686, 693
BssMI GATC 3 cut(s) 413, 430, 472
BssNI GRCGYC 3 cut(s) 300, 545, 774
BssT1I CCWWGG 2 cut(s) 258, 693
Bst4CI ACNGT 2 cut(s) 310, 427
Bst6I CTCTTC 1 cut(s) 401
BstACI GRCGYC 3 cut(s) 300, 545, 774
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 156, 304
BstDEI CTNAG 2 cut(s) 405, 599
BstDSI CCRYGG 3 cut(s) 258, 686, 693
BstF5I GGATG 3 cut(s) 170, 270, 318
BstFNI CGCG 1 cut(s) 794
BstHHI GCGC 2 cut(s) 791, 796
BstKTI GATC 3 cut(s) 416, 433, 475
BstMAI GTCTC 2 cut(s) 208, 316
BstMBI GATC 3 cut(s) 413, 430, 472
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 2 cut(s) 698, 802
BstNSI RCATGY 1 cut(s) 787
BstSLI GKGCMC 1 cut(s) 627
BstUI CGCG 1 cut(s) 794
BstV1I GCAGC 2 cut(s) 19, 80
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BstZI CGGCCG 1 cut(s) 494
Bsu36I CCTNAGG 1 cut(s) 405
BsuRI GGCC 6 cut(s) 158, 496, 596, 692, 698, 741
BtgI CCRYGG 3 cut(s) 258, 686, 693
BtrI CACGTC 1 cut(s) 534
BtsCI GGATG 3 cut(s) 170, 270, 318
Cac8I GCNNGC 2 cut(s) 156, 304
CfoI GCGC 2 cut(s) 791, 796
Cfr10I RCCGGY 1 cut(s) 496
Cfr13I GGNCC 8 cut(s) 126, 490, 511, 595, 618, 690, 711, 740
CseI GACGC 4 cut(s) 308, 625, 712, 763
Csp6I GTAC 2 cut(s) 235, 377
CviAII CATG 4 cut(s) 232, 259, 694, 784
CviQI GTAC 2 cut(s) 235, 377
DdeI CTNAG 2 cut(s) 405, 599
DpnI GATC 3 cut(s) 415, 432, 474
DpnII GATC 3 cut(s) 413, 430, 472
DraI TTTAAA 1 cut(s) 525
EaeI YGGCCR 2 cut(s) 494, 696
EagI CGGCCG 1 cut(s) 494
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
EciI GGCGGA 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 494
Eco130I CCWWGG 2 cut(s) 258, 693
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 5 cut(s) 126, 490, 511, 618, 711
Eco52I CGGCCG 1 cut(s) 494
Eco53kI GAGCTC 1 cut(s) 241
Eco72I CACGTG 1 cut(s) 123
Eco81I CCTNAGG 1 cut(s) 405
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 511
EcoT14I CCWWGG 2 cut(s) 258, 693
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 2 cut(s) 258, 693
Esp3I CGTCTC 2 cut(s) 208, 316
FaeI CATG 4 cut(s) 235, 262, 697, 787
FaiI YATR 4 cut(s) 233, 260, 695, 785
FaqI GGGAC 4 cut(s) 112, 239, 524, 724
FatI CATG 4 cut(s) 231, 258, 693, 783
FauI CCCGC 3 cut(s) 299, 311, 701
FblI GTMKAC 4 cut(s) 78, 102, 354, 639
Fnu4HI GCNGC 4 cut(s) 8, 69, 699, 792
FokI GGATG 3 cut(s) 157, 257, 305
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 4 cut(s) 8, 69, 699, 792
FspBI CTAG 2 cut(s) 59, 458
GlaI GCGC 2 cut(s) 790, 795
GluI GCNGC 4 cut(s) 8, 69, 699, 792
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 6 cut(s) 158, 496, 596, 692, 698, 741
HapII CCGG 3 cut(s) 493, 497, 537
HgaI GACGC 4 cut(s) 308, 625, 712, 763
HhaI GCGC 2 cut(s) 791, 796
Hin1I GRCGYC 3 cut(s) 300, 545, 774
Hin1II CATG 4 cut(s) 235, 262, 697, 787
Hin6I GCGC 2 cut(s) 789, 794
HinP1I GCGC 2 cut(s) 789, 794
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 3 cut(s) 335, 454, 649
HpaII CCGG 3 cut(s) 493, 497, 537
HphI GGTGA 2 cut(s) 43, 769
Hpy166II GTNNAC 6 cut(s) 79, 103, 355, 586, 640, 648
Hpy188I TCNGA 2 cut(s) 505, 555
Hpy188III TCNNGA 2 cut(s) 407, 537
Hpy8I GTNNAC 6 cut(s) 79, 103, 355, 586, 640, 648
Hpy99I CGWCG 7 cut(s) 302, 356, 431, 547, 550, 707, 803
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 2 cut(s) 310, 427
HpyCH4IV ACGT 4 cut(s) 122, 533, 545, 591
HpyCH4V TGCA 2 cut(s) 26, 568
HpyF10VI GCNNNNNNNGC 2 cut(s) 698, 802
HpyF3I CTNAG 2 cut(s) 405, 599
HpySE526I ACGT 4 cut(s) 122, 533, 545, 591
Hsp92I GRCGYC 3 cut(s) 300, 545, 774
Hsp92II CATG 4 cut(s) 235, 262, 697, 787
HspAI GCGC 2 cut(s) 789, 794
KflI GGGWCCC 1 cut(s) 511
Kpn2I TCCGGA 1 cut(s) 536
Kzo9I GATC 3 cut(s) 413, 430, 472
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 4 cut(s) 4, 246, 266, 556
Lsp1109I GCAGC 2 cut(s) 19, 80
LweI GCATC 2 cut(s) 134, 192
MaeI CTAG 2 cut(s) 59, 458
MaeII ACGT 4 cut(s) 122, 533, 545, 591
MaeIII GTNAC 1 cut(s) 775
MalI GATC 3 cut(s) 415, 432, 474
MboI GATC 3 cut(s) 413, 430, 472
MboII GAAGA 2 cut(s) 340, 388
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 3 cut(s) 243, 627, 807
MluCI AATT 2 cut(s) 85, 731
MlyI GAGTC 2 cut(s) 463, 643
MroI TCCGGA 1 cut(s) 536
MseI TTAA 4 cut(s) 483, 524, 758, 814
MslI CAYNNNNRTG 1 cut(s) 782
MspA1I CMGCKG 2 cut(s) 154, 478
MspI CCGG 3 cut(s) 493, 497, 537
MteI GCGCNGCGC 1 cut(s) 792
MunI CAATTG 1 cut(s) 85
MvnI CGCG 1 cut(s) 794
MwoI GCNNNNNNNGC 2 cut(s) 698, 802
NcoI CCATGG 2 cut(s) 258, 693
NdeII GATC 3 cut(s) 413, 430, 472
NlaIII CATG 4 cut(s) 235, 262, 697, 787
NlaIV GGNNCC 8 cut(s) 128, 268, 474, 491, 512, 513, 552, 712
NmeAIII GCCGAG 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 775
NspI RCATGY 1 cut(s) 787
PciI ACATGT 1 cut(s) 783
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 5 cut(s) 306, 447, 539, 551, 759
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 4 cut(s) 9, 70, 700, 793
PleI GAGTC 2 cut(s) 462, 643
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 2 cut(s) 462, 643
Ppu21I YACGTR 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 511
PscI ACATGT 1 cut(s) 783
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 511
PspCI CACGTG 1 cut(s) 123
PspN4I GGNNCC 8 cut(s) 128, 268, 474, 491, 512, 513, 552, 712
PspPI GGNCC 8 cut(s) 126, 490, 511, 595, 618, 690, 711, 740
PspPPI RGGWCCY 1 cut(s) 511
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 154
RsaI GTAC 2 cut(s) 236, 378
RsaNI GTAC 2 cut(s) 235, 377
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 243
SalI GTCGAC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 4 cut(s) 483, 524, 758, 814
SatI GCNGC 4 cut(s) 8, 69, 699, 792
Sau3AI GATC 3 cut(s) 413, 430, 472
Sau96I GGNCC 8 cut(s) 126, 490, 511, 595, 618, 690, 711, 740
SchI GAGTC 2 cut(s) 463, 643
SduI GDGCHC 3 cut(s) 243, 627, 807
SfaNI GCATC 2 cut(s) 134, 192
SinI GGWCC 5 cut(s) 126, 490, 511, 618, 711
SmiMI CAYNNNNRTG 1 cut(s) 782
Sse9I AATT 2 cut(s) 85, 731
SsiI CCGC 9 cut(s) 139, 292, 304, 325, 476, 699, 708, 727, 792
SspMI CTAG 2 cut(s) 59, 458
SstI GAGCTC 1 cut(s) 243
StyI CCWWGG 2 cut(s) 258, 693
TaaI ACNGT 2 cut(s) 310, 427
TaiI ACGT 4 cut(s) 125, 536, 548, 594
TaqI TCGA 6 cut(s) 297, 354, 429, 441, 735, 762
TasI AATT 2 cut(s) 85, 731
TauI GCSGC 2 cut(s) 701, 794
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 4 cut(s) 483, 524, 758, 814
Tru9I TTAA 4 cut(s) 483, 524, 758, 814
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 2 cut(s) 7, 68
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 1 cut(s) 427
TspGWI ACGGA 4 cut(s) 304, 426, 610, 626
VpaK11BI GGWCC 5 cut(s) 126, 490, 511, 618, 711
XapI RAATTY 1 cut(s) 731
XceI RCATGY 1 cut(s) 787
XmiI GTMKAC 4 cut(s) 78, 102, 354, 639
XspI CTAG 2 cut(s) 59, 458
ZraI GACGTC 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.