Rw3G025210

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000677
Physical Location & Seq
Reverse (-)
33293126 .. 33295102
1977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025210.1

Sequence Viewer

Length: 1551 bp
ATGGAAGAGCTGAAGCCAACCCCCGCCAACTCATCCCCGCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGAACACACCATCTACACGTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCATCCGACGCGGCCACGTCCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGACGCCCTTCATCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACGACGATGCACCCGCACCCCATCCCAACTCCTCCGACTCATCCCCCTTCTGCGCCACGTACGAGGAAACGGTGGACAGCGGCGATCCGGACTTCCAGTGGGCCCTGCCGGAGAACGAGTGGGACCCGATGACACTGAACTACACTTCCGGCACGACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTGGGAGGGACCAACGTCTGCGTCCGCAAATTCGACGCGCCTGCCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACCAATGTACCGAACAGCGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCTTCCGCAGCGGTTCTGTCTCGGACTGAGTCGCTGGGATTCGTGGTGAGCCACGGGTACGGGTTAACCGAGACGGCGGGGCTGGTGGTTTCCGTGCCTGCGTGGAAGCCCGGGTGGAATAAGTTTCCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGGGTGAGGACGGTGGCCATGACCCGGGTGGACGTGGTGGATCCGGATACGGGGGTGCCGGTTAAACGCGACGGTTCGTCGCTTGGGGAGGTGGTTTTCAGAGGCGGGTGTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATCAAAGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACTCGGCGGTGAACGAGGCTGCCGTGGTGGCGAGGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCAAAGACGGTGGTGTTTATTGAGGAGCTGCCGAAGACGTCGACGGGGAAAGTTCAGAAGTTCGTGCTGAGAGAGATGGCGGCCCAGTGTAACTTGATTCGCGGCTCGTTGCGGAACGGGTACGCAGTACGTCAGTCTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

516

Amino Acids

56.48

Weight (kDa)

6.24

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 123 - 359 5e-50 AMP-binding enzyme
AMP-binding_C PF13193 409 - 486 2.7e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1448
AccB1I GGYRCC 2 cut(s) 1003, 1405
AccB7I CCANNNNNTGG 1 cut(s) 582
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 3 cut(s) 78, 210, 1448
AccII CGCG 6 cut(s) 153, 189, 647, 1017, 1316, 1509
AccIII TCCGGA 2 cut(s) 382, 991
AclWI GGATC 5 cut(s) 374, 608, 983, 996, 1173
AcoI YGGCCR 3 cut(s) 190, 963, 1095
AcsI RAATTY 2 cut(s) 638, 1396
AcuI CTGAAG 2 cut(s) 32, 956
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 4 cut(s) 240, 681, 1307, 1445
AfaI GTAC 8 cut(s) 356, 531, 596, 732, 845, 1376, 1529, 1536
AfiI CCNNNNNNNGG 5 cut(s) 523, 582, 615, 972, 998
AflIII ACRYGT 2 cut(s) 120, 690
AhdI GACNNNNNGTC 4 cut(s) 138, 542, 680, 1024
AjiI CACGTC 5 cut(s) 196, 982, 1135, 1189, 1339
AloI GAACNNNNNNTCC 2 cut(s) 425, 457
AluBI AGCT 6 cut(s) 10, 225, 588, 782, 1390, 1435
AluI AGCT 6 cut(s) 10, 225, 588, 782, 1390, 1435
Alw21I GWGCWC 1 cut(s) 784
Alw26I GTCTC 3 cut(s) 810, 851, 1298
AlwI GGATC 5 cut(s) 374, 608, 983, 996, 1173
Ama87I CYCGRG 2 cut(s) 896, 972
Aor13HI TCCGGA 2 cut(s) 382, 991
AoxI GGCC 8 cut(s) 190, 396, 525, 963, 1095, 1283, 1349, 1488
ApaI GGGCCC 1 cut(s) 400
ApeKI GCWGC 5 cut(s) 794, 1218, 1268, 1390, 1435
ApoI RAATTY 2 cut(s) 638, 1396
AspLEI GCGC 4 cut(s) 350, 649, 703, 1316
AspS9I GGNCC 9 cut(s) 396, 397, 418, 472, 525, 618, 709, 760, 1489
AsuC2I CCSGG 6 cut(s) 758, 897, 898, 973, 974, 1151
AsuHPI GGTGA 6 cut(s) 34, 493, 844, 964, 1153, 1270
AvaI CYCGRG 2 cut(s) 896, 972
AvaII GGWCC 5 cut(s) 418, 472, 618, 709, 760
BaeGI GKGCMC 1 cut(s) 400
BaeI ACNNNNGTAYC 4 cut(s) 714, 747, 987, 1020
BalI TGGCCA 1 cut(s) 965
BamHI GGATCC 1 cut(s) 988
BanI GGYRCC 2 cut(s) 1003, 1405
BanII GRGCYC 3 cut(s) 400, 492, 784
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 1448
Bbv12I GWGCWC 1 cut(s) 784
BbvCI CCTCAGC 1 cut(s) 221
BbvI GCAGC 5 cut(s) 806, 1230, 1255, 1377, 1422
BccI CCATC 5 cut(s) 122, 173, 324, 662, 1477
BceAI ACGGC 9 cut(s) 97, 512, 593, 595, 694, 876, 1110, 1256, 1294
BcgI CGANNNNNNTGC 4 cut(s) 284, 318, 632, 666
BciVI GTATCC 1 cut(s) 988
BclI TGATCA 2 cut(s) 666, 1191
BcnI CCSGG 6 cut(s) 758, 897, 898, 973, 974, 1151
BcoDI GTCTC 3 cut(s) 810, 851, 1298
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 1378
BfuI GTATCC 1 cut(s) 988
BglI GCCNNNNNGGC 1 cut(s) 1277
BmcAI AGTACT 1 cut(s) 1376
Bme1390I CCNGG 6 cut(s) 758, 897, 898, 973, 974, 1151
Bme18I GGWCC 5 cut(s) 418, 472, 618, 709, 760
BmeRI GACNNNNNGTC 4 cut(s) 138, 542, 680, 1024
BmeT110I CYCGRG 2 cut(s) 896, 972
BmgBI CACGTC 5 cut(s) 196, 982, 1135, 1189, 1339
BmgT120I GGNCC 9 cut(s) 396, 397, 418, 472, 525, 618, 709, 760, 1489
BmiI GGNNCC 9 cut(s) 128, 398, 419, 420, 619, 755, 990, 1005, 1407
BmrFI CCNGG 6 cut(s) 758, 897, 898, 973, 974, 1151
BmrI ACTGGG 2 cut(s) 532, 1486
BmsI GCATC 4 cut(s) 134, 189, 292, 1134
BmuI ACTGGG 2 cut(s) 532, 1486
BoxI GACNNNNGTC 1 cut(s) 623
BpiI GAAGAC 1 cut(s) 1448
BpmI CTGGAG 2 cut(s) 604, 1184
Bpu10I CCTNAGC 1 cut(s) 221
BpuEI CTTGAG 1 cut(s) 1234
BpuMI CCSGG 6 cut(s) 758, 897, 898, 973, 974, 1151
BsaAI YACGTR 2 cut(s) 123, 354
BsaHI GRCGYC 4 cut(s) 240, 681, 1307, 1445
BsaJI CCNNGG 8 cut(s) 175, 462, 609, 838, 896, 972, 1182, 1272
BsaWI WCCGGW 2 cut(s) 382, 991
BsaXI ACNNNNNCTCC 6 cut(s) 398, 428, 1217, 1247, 1295, 1325
Bsc4I CCNNNNNNNGG 5 cut(s) 523, 582, 615, 972, 998
Bse118I RCCGGY 3 cut(s) 703, 1006, 1127
Bse1I ACTGG 4 cut(s) 391, 527, 1098, 1492
BseAI TCCGGA 2 cut(s) 382, 991
BseDI CCNNGG 8 cut(s) 175, 462, 609, 838, 896, 972, 1182, 1272
BseGI GGATG 4 cut(s) 32, 180, 316, 335
BseLI CCNNNNNNNGG 5 cut(s) 523, 582, 615, 972, 998
BseMII CTCAG 3 cut(s) 235, 804, 1466
BseNI ACTGG 4 cut(s) 391, 527, 1098, 1492
BseRI GAGGAG 6 cut(s) 160, 191, 269, 316, 506, 1445
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 5 cut(s) 806, 1230, 1255, 1377, 1422
BseYI CCCAGC 1 cut(s) 820
Bsh1236I CGCG 6 cut(s) 153, 189, 647, 1017, 1316, 1509
BshFI GGCC 8 cut(s) 192, 398, 527, 965, 1097, 1285, 1351, 1490
BshNI GGYRCC 2 cut(s) 1003, 1405
BsiHKAI GWGCWC 1 cut(s) 784
BsiHKCI CYCGRG 2 cut(s) 896, 972
BsiWI CGTACG 2 cut(s) 354, 594
BslFI GGGAC 2 cut(s) 431, 631
BslI CCNNNNNNNGG 5 cut(s) 523, 582, 615, 972, 998
BsmAI GTCTC 3 cut(s) 810, 851, 1298
BsmBI CGTCTC 2 cut(s) 851, 1298
BsmFI GGGAC 2 cut(s) 431, 631
BsnI GGCC 8 cut(s) 192, 398, 527, 965, 1097, 1285, 1351, 1490
BsoBI CYCGRG 2 cut(s) 896, 972
Bsp120I GGGCCC 1 cut(s) 396
Bsp1286I GDGCHC 3 cut(s) 400, 492, 784
Bsp13I TCCGGA 2 cut(s) 382, 991
Bsp143I GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
BspANI GGCC 8 cut(s) 192, 398, 527, 965, 1097, 1285, 1351, 1490
BspCNI CTCAG 3 cut(s) 234, 805, 1467
BspEI TCCGGA 2 cut(s) 382, 991
BspFNI CGCG 6 cut(s) 153, 189, 647, 1017, 1316, 1509
BspHI TCATGA 1 cut(s) 768
BspLI GGNNCC 9 cut(s) 128, 398, 419, 420, 619, 755, 990, 1005, 1407
BspMAI CTGCAG 1 cut(s) 1382
BspPI GGATC 5 cut(s) 374, 608, 983, 996, 1173
BspQI GCTCTTC 1 cut(s) 497
BspT107I GGYRCC 2 cut(s) 1003, 1405
BsrBI CCGCTC 1 cut(s) 40
BsrFI RCCGGY 3 cut(s) 703, 1006, 1127
BsrI ACTGG 4 cut(s) 391, 527, 1098, 1492
BssAI RCCGGY 3 cut(s) 703, 1006, 1127
BssECI CCNNGG 8 cut(s) 175, 462, 609, 838, 896, 972, 1182, 1272
BssMI GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
BssNI GRCGYC 4 cut(s) 240, 681, 1307, 1445
BssT1I CCWWGG 2 cut(s) 462, 1182
Bst4CI ACNGT 8 cut(s) 76, 140, 367, 505, 961, 1022, 1115, 1417
Bst6I CTCTTC 3 cut(s) 209, 257, 497
BstACI GRCGYC 4 cut(s) 240, 681, 1307, 1445
BstBAI YACGTR 2 cut(s) 123, 354
BstC8I GCNNGC 3 cut(s) 651, 743, 885
BstDEI CTNAG 3 cut(s) 221, 813, 1475
BstDSI CCRYGG 3 cut(s) 609, 838, 1272
BstF5I GGATG 4 cut(s) 32, 180, 316, 335
BstFNI CGCG 6 cut(s) 153, 189, 647, 1017, 1316, 1509
BstHHI GCGC 4 cut(s) 350, 649, 703, 1316
BstKTI GATC 8 cut(s) 382, 603, 669, 768, 991, 1170, 1181, 1194
BstMAI GTCTC 3 cut(s) 810, 851, 1298
BstMBI GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
BstMWI GCNNNNNNNGC 2 cut(s) 186, 1277
BstNSI RCATGY 2 cut(s) 694, 721
BstPAI GACNNNNGTC 1 cut(s) 623
BstSCI CCNGG 6 cut(s) 756, 895, 896, 971, 972, 1149
BstSFI CTRYAG 1 cut(s) 1378
BstSLI GKGCMC 1 cut(s) 400
BstUI CGCG 6 cut(s) 153, 189, 647, 1017, 1316, 1509
BstV1I GCAGC 5 cut(s) 806, 1230, 1255, 1377, 1422
BstV2I GAAGAC 1 cut(s) 1448
BstX2I RGATCY 2 cut(s) 988, 1178
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 2 cut(s) 988, 1178
BsuI GTATCC 1 cut(s) 988
BsuRI GGCC 8 cut(s) 192, 398, 527, 965, 1097, 1285, 1351, 1490
BtgI CCRYGG 3 cut(s) 609, 838, 1272
BtgZI GCGATG 1 cut(s) 274
BtrI CACGTC 5 cut(s) 196, 982, 1135, 1189, 1339
BtsCI GGATG 4 cut(s) 32, 180, 316, 335
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 4 cut(s) 398, 428, 475, 1499
Cac8I GCNNGC 3 cut(s) 651, 743, 885
CciI TCATGA 1 cut(s) 768
CfoI GCGC 4 cut(s) 350, 649, 703, 1316
Cfr10I RCCGGY 3 cut(s) 703, 1006, 1127
Cfr13I GGNCC 9 cut(s) 396, 397, 418, 472, 525, 618, 709, 760, 1489
Cfr9I CCCGGG 2 cut(s) 896, 972
CseI GACGC 8 cut(s) 142, 195, 248, 565, 619, 653, 670, 1315
Csp6I GTAC 8 cut(s) 355, 530, 595, 731, 844, 1375, 1528, 1535
CspCI CAANNNNNGTGG 2 cut(s) 1398, 1433
CviAII CATG 6 cut(s) 691, 718, 769, 967, 1105, 1402
CviQI GTAC 8 cut(s) 355, 530, 595, 731, 844, 1375, 1528, 1535
DdeI CTNAG 3 cut(s) 221, 813, 1475
DpnI GATC 8 cut(s) 381, 602, 668, 767, 990, 1169, 1180, 1193
DpnII GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
DriI GACNNNNNGTC 4 cut(s) 138, 542, 680, 1024
EaeI YGGCCR 3 cut(s) 190, 963, 1095
Eam1104I CTCTTC 3 cut(s) 209, 257, 497
Eam1105I GACNNNNNGTC 4 cut(s) 138, 542, 680, 1024
EarI CTCTTC 3 cut(s) 209, 257, 497
EciI GGCGGA 2 cut(s) 774, 1360
Ecl136II GAGCTC 1 cut(s) 782
Eco130I CCWWGG 2 cut(s) 462, 1182
Eco147I AGGCCT 1 cut(s) 1285
Eco24I GRGCYC 3 cut(s) 400, 492, 784
Eco47I GGWCC 5 cut(s) 418, 472, 618, 709, 760
Eco53kI GAGCTC 1 cut(s) 782
Eco57I CTGAAG 2 cut(s) 32, 956
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 2 cut(s) 896, 972
EcoICRI GAGCTC 1 cut(s) 782
EcoO109I RGGNCCY 2 cut(s) 397, 418
EcoT14I CCWWGG 2 cut(s) 462, 1182
EcoT38I GRGCYC 3 cut(s) 400, 492, 784
ErhI CCWWGG 2 cut(s) 462, 1182
Esp3I CGTCTC 2 cut(s) 851, 1298
FaeI CATG 6 cut(s) 694, 721, 772, 970, 1108, 1405
FaiI YATR 8 cut(s) 692, 719, 770, 968, 1106, 1196, 1403, 1549
FaqI GGGAC 2 cut(s) 431, 631
FatI CATG 6 cut(s) 690, 717, 768, 966, 1104, 1401
FauI CCCGC 6 cut(s) 31, 45, 316, 856, 1046, 1193
FbaI TGATCA 2 cut(s) 666, 1191
FblI GTMKAC 3 cut(s) 78, 210, 1448
FokI GGATG 4 cut(s) 19, 167, 303, 322
FriOI GRGCYC 3 cut(s) 400, 492, 784
FspBI CTAG 1 cut(s) 59
GlaI GCGC 4 cut(s) 349, 648, 702, 1315
GsaI CCCAGC 1 cut(s) 824
GsuI CTGGAG 2 cut(s) 604, 1184
HaeIII GGCC 8 cut(s) 192, 398, 527, 965, 1097, 1285, 1351, 1490
HgaI GACGC 8 cut(s) 142, 195, 248, 565, 619, 653, 670, 1315
HhaI GCGC 4 cut(s) 350, 649, 703, 1316
Hin1I GRCGYC 4 cut(s) 240, 681, 1307, 1445
Hin1II CATG 6 cut(s) 694, 721, 772, 970, 1108, 1405
Hin6I GCGC 4 cut(s) 348, 647, 701, 1314
HinP1I GCGC 4 cut(s) 348, 647, 701, 1314
HincII GTYRAC 4 cut(s) 211, 508, 852, 1449
HindII GTYRAC 4 cut(s) 211, 508, 852, 1449
HinfI GANTC 5 cut(s) 332, 815, 825, 1232, 1504
HpaI GTTAAC 1 cut(s) 852
HphI GGTGA 6 cut(s) 34, 493, 844, 964, 1153, 1270
Hpy166II GTNNAC 9 cut(s) 79, 211, 370, 475, 508, 852, 979, 1261, 1449
Hpy188I TCNGA 8 cut(s) 185, 331, 564, 671, 810, 1049, 1077, 1464
Hpy188III TCNNGA 6 cut(s) 383, 553, 763, 769, 992, 1163
Hpy8I GTNNAC 9 cut(s) 79, 211, 370, 475, 508, 852, 979, 1261, 1449
HpyAV CCTTC 5 cut(s) 102, 254, 352, 798, 1354
HpyCH4III ACNGT 8 cut(s) 76, 140, 367, 505, 961, 1022, 1115, 1417
HpyCH4V TGCA 4 cut(s) 305, 1104, 1147, 1380
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 1277
HpyF3I CTNAG 3 cut(s) 221, 813, 1475
Hsp92I GRCGYC 4 cut(s) 240, 681, 1307, 1445
Hsp92II CATG 6 cut(s) 694, 721, 772, 970, 1108, 1405
HspAI GCGC 4 cut(s) 348, 647, 701, 1314
KflI GGGWCCC 1 cut(s) 418
Kpn2I TCCGGA 2 cut(s) 382, 991
Ksp22I TGATCA 2 cut(s) 666, 1191
KspAI GTTAAC 1 cut(s) 852
Kzo9I GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
LguI GCTCTTC 1 cut(s) 497
LmnI GCTCC 6 cut(s) 126, 230, 585, 779, 787, 1432
Lsp1109I GCAGC 5 cut(s) 806, 1230, 1255, 1377, 1422
LweI GCATC 4 cut(s) 134, 189, 292, 1134
MaeI CTAG 1 cut(s) 59
MaeIII GTNAC 2 cut(s) 682, 1496
MalI GATC 8 cut(s) 381, 602, 668, 767, 990, 1169, 1180, 1193
MbiI CCGCTC 1 cut(s) 40
MboI GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
MboII GAAGA 5 cut(s) 17, 196, 244, 484, 1453
MflI RGATCY 2 cut(s) 988, 1178
MhlI GDGCHC 3 cut(s) 400, 492, 784
MlsI TGGCCA 1 cut(s) 965
MluCI AATT 2 cut(s) 638, 1396
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 3 cut(s) 326, 824, 1241
MmeI TCCRAC 2 cut(s) 208, 354
Mox20I TGGCCA 1 cut(s) 965
MroI TCCGGA 2 cut(s) 382, 991
MscI TGGCCA 1 cut(s) 965
MseI TTAA 2 cut(s) 851, 1011
MslI CAYNNNNRTG 1 cut(s) 689
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 2 cut(s) 375, 797
MspR9I CCNGG 6 cut(s) 758, 897, 898, 973, 974, 1151
MvnI CGCG 6 cut(s) 153, 189, 647, 1017, 1316, 1509
MwoI GCNNNNNNNGC 2 cut(s) 186, 1277
NciI CCSGG 6 cut(s) 758, 897, 898, 973, 974, 1151
NdeII GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
NlaIII CATG 6 cut(s) 694, 721, 772, 970, 1108, 1405
NlaIV GGNNCC 9 cut(s) 128, 398, 419, 420, 619, 755, 990, 1005, 1407
NmeAIII GCCGAG 3 cut(s) 30, 156, 1232
NmuCI GTSAC 1 cut(s) 682
NspI RCATGY 2 cut(s) 694, 721
PagI TCATGA 1 cut(s) 768
PceI AGGCCT 1 cut(s) 1285
PciI ACATGT 1 cut(s) 690
PciSI GCTCTTC 1 cut(s) 497
PcsI WCGNNNNNNNCGW 6 cut(s) 297, 525, 852, 926, 1269, 1313
PfeI GAWTC 2 cut(s) 825, 1504
Pfl23II CGTACG 2 cut(s) 354, 594
PflFI GACNNNGTC 1 cut(s) 814
PflMI CCANNNNNTGG 1 cut(s) 582
PleI GAGTC 3 cut(s) 326, 823, 1240
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 3 cut(s) 326, 823, 1240
Ppu21I YACGTR 2 cut(s) 123, 354
PpuMI RGGWCCY 1 cut(s) 418
PscI ACATGT 1 cut(s) 690
PshAI GACNNNNGTC 1 cut(s) 623
Psp124BI GAGCTC 1 cut(s) 784
Psp5II RGGWCCY 1 cut(s) 418
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 820
PspLI CGTACG 2 cut(s) 354, 594
PspN4I GGNNCC 9 cut(s) 128, 398, 419, 420, 619, 755, 990, 1005, 1407
PspOMI GGGCCC 1 cut(s) 396
PspPI GGNCC 9 cut(s) 396, 397, 418, 472, 525, 618, 709, 760, 1489
PspPPI RGGWCCY 1 cut(s) 418
PstI CTGCAG 1 cut(s) 1382
PsuI RGATCY 2 cut(s) 988, 1178
PsyI GACNNNGTC 1 cut(s) 814
RsaI GTAC 8 cut(s) 356, 531, 596, 732, 845, 1376, 1529, 1536
RsaNI GTAC 8 cut(s) 355, 530, 595, 731, 844, 1375, 1528, 1535
RseI CAYNNNNRTG 1 cut(s) 689
SacI GAGCTC 1 cut(s) 784
SalI GTCGAC 2 cut(s) 209, 1447
SapI GCTCTTC 1 cut(s) 497
SaqAI TTAA 2 cut(s) 851, 1011
Sau3AI GATC 8 cut(s) 379, 600, 666, 765, 988, 1167, 1178, 1191
Sau96I GGNCC 9 cut(s) 396, 397, 418, 472, 525, 618, 709, 760, 1489
ScaI AGTACT 1 cut(s) 1376
SchI GAGTC 3 cut(s) 326, 824, 1241
ScrFI CCNGG 6 cut(s) 758, 897, 898, 973, 974, 1151
SduI GDGCHC 3 cut(s) 400, 492, 784
SfaNI GCATC 4 cut(s) 134, 189, 292, 1134
SfcI CTRYAG 1 cut(s) 1378
SgrAI CRCCGGYG 2 cut(s) 703, 1127
SgrDI CGTCGACG 1 cut(s) 1447
SinI GGWCC 5 cut(s) 418, 472, 618, 709, 760
SmaI CCCGGG 2 cut(s) 898, 974
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 1213
SmoI CTYRAG 1 cut(s) 1213
Sse9I AATT 2 cut(s) 638, 1396
SseBI AGGCCT 1 cut(s) 1285
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 784
StuI AGGCCT 1 cut(s) 1285
StyD4I CCNGG 6 cut(s) 756, 895, 896, 971, 972, 1149
StyI CCWWGG 2 cut(s) 462, 1182
TaaI ACNGT 8 cut(s) 76, 140, 367, 505, 961, 1022, 1115, 1417
TaqI TCGA 7 cut(s) 142, 210, 237, 519, 642, 941, 1448
TaqII GACCGA 1 cut(s) 78
TasI AATT 2 cut(s) 638, 1396
TatI WGTACW 1 cut(s) 1374
TauI GCSGC 6 cut(s) 71, 192, 378, 608, 1490, 1512
TfiI GAWTC 2 cut(s) 825, 1504
Tru1I TTAA 2 cut(s) 851, 1011
Tru9I TTAA 2 cut(s) 851, 1011
TscAI CASTG 4 cut(s) 398, 435, 482, 1499
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 5 cut(s) 794, 1218, 1268, 1390, 1435
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 4 cut(s) 236, 484, 1121, 1390
TspGWI ACGGA 3 cut(s) 868, 935, 1370
TspMI CCCGGG 2 cut(s) 896, 972
TspRI CASTG 4 cut(s) 398, 435, 482, 1499
Tth111I GACNNNGTC 1 cut(s) 814
Van91I CCANNNNNTGG 1 cut(s) 582
VpaK11BI GGWCC 5 cut(s) 418, 472, 618, 709, 760
XapI RAATTY 2 cut(s) 638, 1396
XceI RCATGY 2 cut(s) 694, 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmaI CCCGGG 2 cut(s) 896, 972
XmiI GTMKAC 3 cut(s) 78, 210, 1448
XspI CTAG 1 cut(s) 59
ZraI GACGTC 1 cut(s) 1446
ZrmI AGTACT 1 cut(s) 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.