Rh3AG282200

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000677
Physical Location & Seq
Reverse (-)
32945472 .. 32960013
14542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG282200.1

Sequence Viewer

Length: 696 bp
ATGAGCGTGAAACGAAACGGGTCGGATGTTGGAGAAATAGTCATGCGAGGCGGGTGCGTCATGTTGGGTTACCTCAAAGACCCGGAAACGACGAGCGAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGCTCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAATGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTCAGGCCGAGGCCGACGGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCAAAGACGTCGACGGGGAAGATTCAGAAATCTATGTTGAGAGAGATTGCGAAAAATATTGGGACTACATCAACGACGAGAGTTCAATTGGCTTGTAAAGGAATAGAGGAAACTATTGAAGACGATGAAGAACTCAAAGTGGGAATGCAAGTACACTCTGATGATGAGCCTTACAATTTATATAACACCTATGCTTTGAAGAAGGGTTTCAGTGTTCATAAAGGACATATTCGAAGAGATGCATCAAATAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

231

Amino Acids

25.65

Weight (kDa)

5.53

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 76 - 153 1.5e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 138, 342, 449
AccB1I GGYRCC 1 cut(s) 406
AccI GTMKAC 1 cut(s) 449
AccII CGCG 2 cut(s) 257, 317
AciI CCGC 5 cut(s) 51, 201, 255, 257, 269
AclWI GGATC 1 cut(s) 183
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 1 cut(s) 397
AcyI GRCGYC 4 cut(s) 135, 308, 339, 446
AfaI GTAC 1 cut(s) 591
AflII CTTAAG 1 cut(s) 214
AgsI TTSAA 4 cut(s) 427, 524, 557, 637
AjiI CACGTC 1 cut(s) 190
AluBI AGCT 3 cut(s) 335, 391, 436
AluI AGCT 3 cut(s) 335, 391, 436
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 1 cut(s) 183
AoxI GGCC 3 cut(s) 270, 344, 350
ApeKI GCWGC 3 cut(s) 219, 391, 436
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 1 cut(s) 317
AsuC2I CCSGG 2 cut(s) 83, 152
AsuHPI GGTGA 1 cut(s) 271
AsuII TTCGAA 1 cut(s) 670
BanI GGYRCC 1 cut(s) 406
BbsI GAAGAC 2 cut(s) 419, 564
BbvI GCAGC 3 cut(s) 231, 378, 423
BceAI ACGGC 2 cut(s) 257, 295
BcgI CGANNNNNNTGC 2 cut(s) 36, 70
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 2 cut(s) 83, 152
BcoDI GTCTC 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 379
BfrI CTTAAG 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 278
BisI GCNGC 5 cut(s) 220, 255, 270, 392, 437
BlpI GCTNAGC 1 cut(s) 282
BlsI GCNGC 5 cut(s) 221, 256, 271, 393, 438
Bme1390I CCNGG 2 cut(s) 83, 152
BmgBI CACGTC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 2 cut(s) 83, 152
BmsI GCATC 2 cut(s) 667, 689
BpiI GAAGAC 2 cut(s) 419, 564
BpmI CTGGAG 1 cut(s) 185
Bpu1102I GCTNAGC 1 cut(s) 282
Bpu14I TTCGAA 1 cut(s) 670
BpuMI CCSGG 2 cut(s) 83, 152
BsaHI GRCGYC 4 cut(s) 135, 308, 339, 446
BsaJI CCNNGG 4 cut(s) 183, 255, 273, 347
BsaXI ACNNNNNCTCC 6 cut(s) 24, 54, 218, 248, 296, 326
Bse118I RCCGGY 1 cut(s) 128
BseDI CCNNGG 4 cut(s) 183, 255, 273, 347
BseGI GGATG 1 cut(s) 31
BseX3I CGGCCG 1 cut(s) 270
BseXI GCAGC 3 cut(s) 231, 378, 423
Bsh1236I CGCG 2 cut(s) 257, 317
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 3 cut(s) 272, 346, 352
BshNI GGYRCC 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 273
BsiSI CCGG 3 cut(s) 83, 129, 152
BslFI GGGAC 1 cut(s) 514
BsmAI GTCTC 1 cut(s) 299
BsmBI CGTCTC 1 cut(s) 299
BsmFI GGGAC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 3 cut(s) 272, 346, 352
Bsp119I TTCGAA 1 cut(s) 670
Bsp143I GATC 2 cut(s) 175, 192
Bsp1720I GCTNAGC 1 cut(s) 282
BspACI CCGC 5 cut(s) 51, 201, 255, 257, 269
BspANI GGCC 3 cut(s) 272, 346, 352
BspFNI CGCG 2 cut(s) 257, 317
BspLI GGNNCC 1 cut(s) 408
BspPI GGATC 1 cut(s) 183
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 406
BspTI CTTAAG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 4 cut(s) 183, 255, 273, 347
BssMI GATC 2 cut(s) 175, 192
BssNI GRCGYC 4 cut(s) 135, 308, 339, 446
BssT1I CCWWGG 1 cut(s) 183
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 1 cut(s) 667
BstACI GRCGYC 4 cut(s) 135, 308, 339, 446
BstAFI CTTAAG 1 cut(s) 214
BstBI TTCGAA 1 cut(s) 670
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 2 cut(s) 255, 273
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 1 cut(s) 31
BstFNI CGCG 2 cut(s) 257, 317
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 2 cut(s) 178, 195
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 2 cut(s) 175, 192
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 81, 150
BstSFI CTRYAG 1 cut(s) 379
BstUI CGCG 2 cut(s) 257, 317
BstV1I GCAGC 3 cut(s) 231, 378, 423
BstV2I GAAGAC 2 cut(s) 419, 564
BstZI CGGCCG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 272, 346, 352
BtgI CCRYGG 2 cut(s) 255, 273
BtrI CACGTC 1 cut(s) 190
BtsCI GGATG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 655
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 1 cut(s) 128
Cfr42I CCGCGG 1 cut(s) 258
CseI GACGC 2 cut(s) 46, 316
Csp6I GTAC 1 cut(s) 590
CviAII CATG 3 cut(s) 43, 61, 403
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 1 cut(s) 282
DpnI GATC 2 cut(s) 177, 194
DpnII GATC 2 cut(s) 175, 192
EaeI YGGCCR 1 cut(s) 270
EagI CGGCCG 1 cut(s) 270
Eam1104I CTCTTC 1 cut(s) 667
EarI CTCTTC 1 cut(s) 667
EclXI CGGCCG 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 183
Eco52I CGGCCG 1 cut(s) 270
Eco91I GGTNACC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 682
ErhI CCWWGG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 299
FaeI CATG 3 cut(s) 46, 64, 406
FaqI GGGAC 1 cut(s) 514
FatI CATG 3 cut(s) 42, 60, 402
FauI CCCGC 2 cut(s) 44, 194
FbaI TGATCA 1 cut(s) 192
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 5 cut(s) 220, 255, 270, 392, 437
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 5 cut(s) 220, 255, 270, 392, 437
GlaI GCGC 1 cut(s) 316
GluI GCNGC 5 cut(s) 220, 255, 270, 392, 437
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 3 cut(s) 272, 346, 352
HapII CCGG 3 cut(s) 83, 129, 152
HgaI GACGC 2 cut(s) 46, 316
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 4 cut(s) 135, 308, 339, 446
Hin1II CATG 3 cut(s) 46, 64, 406
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 2 cut(s) 233, 460
HpaII CCGG 3 cut(s) 83, 129, 152
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 3 cut(s) 262, 450, 592
Hpy188I TCNGA 4 cut(s) 25, 465, 598, 695
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 3 cut(s) 262, 450, 592
Hpy99I CGWCG 7 cut(s) 94, 137, 140, 358, 451, 454, 517
HpyAV CCTTC 2 cut(s) 355, 634
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 4 cut(s) 135, 189, 339, 446
HpyCH4V TGCA 3 cut(s) 148, 586, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 4 cut(s) 135, 189, 339, 446
Hsp92I GRCGYC 4 cut(s) 135, 308, 339, 446
Hsp92II CATG 3 cut(s) 46, 64, 406
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 192
KspI CCGCGG 1 cut(s) 258
Kzo9I GATC 2 cut(s) 175, 192
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 9 cut(s) 96, 116, 142, 149, 165, 283, 300, 328, 341
Lsp1109I GCAGC 3 cut(s) 231, 378, 423
LweI GCATC 2 cut(s) 667, 689
MaeII ACGT 4 cut(s) 135, 189, 339, 446
MaeIII GTNAC 1 cut(s) 68
MalI GATC 2 cut(s) 177, 194
MboI GATC 2 cut(s) 175, 192
MboII GAAGA 6 cut(s) 424, 469, 569, 578, 649, 684
MfeI CAATTG 1 cut(s) 524
MluCI AATT 3 cut(s) 397, 524, 613
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 7 cut(s) 41, 83, 220, 259, 342, 377, 538
Mph1103I ATGCAT 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 644
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 257
MspCI CTTAAG 1 cut(s) 214
MspI CCGG 3 cut(s) 83, 129, 152
MspR9I CCNGG 2 cut(s) 83, 152
MunI CAATTG 1 cut(s) 524
Mva1269I GAATGC 1 cut(s) 588
MvnI CGCG 2 cut(s) 257, 317
MwoI GCNNNNNNNGC 1 cut(s) 278
NciI CCSGG 2 cut(s) 83, 152
NdeII GATC 2 cut(s) 175, 192
NlaIII CATG 3 cut(s) 46, 64, 406
NlaIV GGNNCC 1 cut(s) 408
NmeAIII GCCGAG 1 cut(s) 372
NsiI ATGCAT 1 cut(s) 682
NspV TTCGAA 1 cut(s) 670
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 314
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 460
PkrI GCNGC 5 cut(s) 221, 256, 271, 393, 438
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PspEI GGTNACC 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 408
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
RseI CAYNNNNRTG 1 cut(s) 597
SacII CCGCGG 1 cut(s) 258
SalI GTCGAC 1 cut(s) 448
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 5 cut(s) 220, 255, 270, 392, 437
Sau3AI GATC 2 cut(s) 175, 192
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 83, 152
SfaNI GCATC 2 cut(s) 667, 689
SfcI CTRYAG 1 cut(s) 379
Sfr303I CCGCGG 1 cut(s) 258
SfuI TTCGAA 1 cut(s) 670
SgrAI CRCCGGYG 1 cut(s) 128
SgrBI CCGCGG 1 cut(s) 258
SgrDI CGTCGACG 1 cut(s) 448
SmiMI CAYNNNNRTG 1 cut(s) 597
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 3 cut(s) 397, 524, 613
SsiI CCGC 5 cut(s) 51, 201, 255, 257, 269
SspI AATATT 1 cut(s) 496
StyD4I CCNGG 2 cut(s) 81, 150
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 4 cut(s) 138, 192, 342, 449
TaqI TCGA 3 cut(s) 231, 449, 670
TasI AATT 3 cut(s) 397, 524, 613
TatI WGTACW 1 cut(s) 589
TauI GCSGC 2 cut(s) 257, 272
TfiI GAWTC 1 cut(s) 460
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 655
TseI GCWGC 3 cut(s) 219, 391, 436
TspDTI ATGAA 3 cut(s) 391, 579, 644
TspGWI ACGGA 1 cut(s) 371
TspRI CASTG 1 cut(s) 655
Vha464I CTTAAG 1 cut(s) 214
XapI RAATTY 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 449
XmnI GAANNNNTTC 1 cut(s) 644
ZraI GACGTC 3 cut(s) 136, 340, 447
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.