Rh3BG320600

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000677
Physical Location & Seq
Reverse (-)
32971170 .. 32977863
6694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG320600.1

Sequence Viewer

Length: 1560 bp
ATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCCGCTCCCTCGTCCTCGACGCCCTCCGTCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACGACGATGCACCCCATCCCAACTCCGACTCATCCCCCTTCTGCGCCACGTATGAGGAAATGGTGGACAGCGGCGATCCGGACTTCCAGTGGGCCCTACCGGAGAACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACTACTTCCTCCCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTGTGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTCGGTGGGACCAACGTTTGCGTCCGCAAATTCGACGCCCATGTCATCTACGACTTGATCAGGCGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACGAATGTACCGAACAATGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCGCCCGCGGCGGTTCTGTCTCGGACTGAGTCGCTGGGATTCGTGGTCAGCCATGGGTACGGGTTGACAGAGACGGCAGGGCCGGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTACCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGATGGTGGCCATGACCCACGTCGACGTGGTGGATCCGGATACTGGTGCACCGGTTAAACGCGACGGTTCATCGCTGGGGGAGGTGGTTTTCAGAGGCGGGTCTGTGATGTTGGGTTACCTGAAAGACCCAAAAGGCACGGCTAGTTGCATGAAAGAAGGTTTGTTTTATACCGGCGACGTGGCAGTGATGCACCCGGACGGGTATCTGGAGATCAAGGATCGATCTAAGGACGTGATCATTAGCGGGGGAGAGAACGTGAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCGGCAGTGAACGAGGCGGCAGTGGTGGCTAGGCCTGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTGGCGCGGAGGCCGACGGAGAAGGAGATAATAGAGTATTGCAGGGGGAAGTTGCCGCATTACATGGTGCCGAAGACGGTGGTGTTTAAGGAGGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTCGTCCTGAGAGAGATGGCGGCCCAGTGTTACTTGATTCGCGGGTCGTTGCGGAACGGGGTTACTCTTTGGGGAGACACTGCACACAGCTTTGAAGCAGAACGCATCAGCCAACTGCCTCCTCTAGCTTTAGACGTCGTGTTCACATGTTTGAGGGTCAAAAACATTTCAAGGCAAGATTATCTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

519

Amino Acids

57.21

Weight (kDa)

6.57

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 326 2.8e-59 AMP-binding enzyme
AMP-binding_C PF13193 376 - 453 3.7e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AatII GACGTC 2 cut(s) 1349, 1506
AccB1I GGYRCC 2 cut(s) 35, 1306
AccB7I CCANNNNNTGG 1 cut(s) 486
AccBSI CCGCTC 1 cut(s) 138
AccI GTMKAC 3 cut(s) 123, 879, 1349
AccII CGCG 7 cut(s) 698, 788, 918, 1154, 1217, 1246, 1410
AccIII TCCGGA 2 cut(s) 286, 892
AclI AACGTT 1 cut(s) 528
AclWI GGATC 5 cut(s) 278, 512, 884, 897, 1083
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 857
AcyI GRCGYC 7 cut(s) 36, 153, 549, 585, 1208, 1346, 1503
AfaI GTAC 5 cut(s) 5, 435, 500, 636, 749
AfiI CCNNNNNNNGG 5 cut(s) 32, 427, 486, 519, 899
AflIII ACRYGT 2 cut(s) 594, 1514
AgeI ACCGGT 1 cut(s) 907
AgsI TTSAA 2 cut(s) 1463, 1539
AhdI GACNNNNNGTC 2 cut(s) 446, 584
AjiI CACGTC 5 cut(s) 877, 883, 1036, 1090, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 8 cut(s) 10, 112, 492, 686, 1235, 1336, 1458, 1496
AluI AGCT 8 cut(s) 10, 112, 492, 686, 1235, 1336, 1458, 1496
Alw21I GWGCWC 3 cut(s) 12, 688, 907
Alw26I GTCTC 6 cut(s) 85, 167, 714, 755, 1199, 1437
Alw44I GTGCAC 1 cut(s) 903
AlwI GGATC 5 cut(s) 278, 512, 884, 897, 1083
Ama87I CYCGRG 1 cut(s) 797
Aor13HI TCCGGA 2 cut(s) 286, 892
AoxI GGCC 7 cut(s) 300, 429, 770, 864, 1184, 1250, 1389
ApaI GGGCCC 1 cut(s) 304
ApaLI GTGCAC 1 cut(s) 903
ApeKI GCWGC 2 cut(s) 1119, 1336
ApoI RAATTY 1 cut(s) 542
AsiGI ACCGGT 1 cut(s) 907
AspLEI GCGC 6 cut(s) 38, 254, 607, 788, 1217, 1246
AsuC2I CCSGG 4 cut(s) 662, 798, 799, 1052
AsuHPI GGTGA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 797
AvaII GGWCC 5 cut(s) 322, 376, 522, 613, 664
BaeGI GKGCMC 2 cut(s) 304, 907
BaeI ACNNNNGTAYC 2 cut(s) 618, 651
BalI TGGCCA 1 cut(s) 866
BamHI GGATCC 1 cut(s) 889
BanI GGYRCC 2 cut(s) 35, 1306
BanII GRGCYC 4 cut(s) 12, 304, 396, 688
BbsI GAAGAC 2 cut(s) 1319, 1349
Bbv12I GWGCWC 3 cut(s) 12, 688, 907
BbvI GCAGC 2 cut(s) 1131, 1323
BccI CCATC 4 cut(s) 59, 231, 853, 1378
BceAI ACGGC 9 cut(s) 5, 23, 416, 497, 499, 598, 780, 1011, 1195
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 2 cut(s) 570, 1092
BcnI CCSGG 4 cut(s) 662, 798, 799, 1052
BcoDI GTCTC 6 cut(s) 85, 167, 714, 755, 1199, 1437
BfaI CTAG 3 cut(s) 999, 1182, 1493
BfoI RGCGCY 1 cut(s) 39
BfuI GTATCC 1 cut(s) 889
Bme1390I CCNGG 4 cut(s) 662, 798, 799, 1052
Bme18I GGWCC 5 cut(s) 322, 376, 522, 613, 664
BmeRI GACNNNNNGTC 2 cut(s) 446, 584
BmeT110I CYCGRG 1 cut(s) 797
BmgBI CACGTC 5 cut(s) 877, 883, 1036, 1090, 1240
BmiI GGNNCC 8 cut(s) 37, 302, 323, 324, 523, 659, 891, 1308
BmrFI CCNGG 4 cut(s) 662, 798, 799, 1052
BmrI ACTGGG 2 cut(s) 436, 1387
BmsI GCATC 3 cut(s) 205, 1035, 1482
BmuI ACTGGG 2 cut(s) 436, 1387
BoxI GACNNNNGTC 1 cut(s) 875
BpiI GAAGAC 2 cut(s) 1319, 1349
BpmI CTGGAG 2 cut(s) 508, 1085
BpuMI CCSGG 4 cut(s) 662, 798, 799, 1052
Bsa29I ATCGAT 1 cut(s) 1078
BsaAI YACGTR 1 cut(s) 258
BsaHI GRCGYC 7 cut(s) 36, 153, 549, 585, 1208, 1346, 1503
BsaJI CCNNGG 4 cut(s) 366, 696, 742, 797
BsaWI WCCGGW 4 cut(s) 286, 307, 892, 907
BsaXI ACNNNNNCTCC 8 cut(s) 302, 332, 1098, 1118, 1128, 1148, 1196, 1226
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 427, 486, 519, 899
Bse118I RCCGGY 5 cut(s) 607, 772, 814, 907, 1028
Bse1I ACTGG 4 cut(s) 295, 431, 904, 1393
BseAI TCCGGA 2 cut(s) 286, 892
BseCI ATCGAT 1 cut(s) 1078
BseDI CCNNGG 4 cut(s) 366, 696, 742, 797
BseGI GGATG 3 cut(s) 223, 239, 864
BseLI CCNNNNNNNGG 5 cut(s) 32, 427, 486, 519, 899
BseMII CTCAG 2 cut(s) 708, 1367
BseNI ACTGG 4 cut(s) 295, 431, 904, 1393
BseRI GAGGAG 7 cut(s) 77, 80, 104, 182, 410, 1346, 1479
BseSI GKGCMC 2 cut(s) 304, 907
BseXI GCAGC 2 cut(s) 1131, 1323
BseYI CCCAGC 2 cut(s) 724, 931
BsgI GTGCAG 1 cut(s) 1434
Bsh1236I CGCG 7 cut(s) 698, 788, 918, 1154, 1217, 1246, 1410
BshFI GGCC 7 cut(s) 302, 431, 772, 866, 1186, 1252, 1391
BshNI GGYRCC 2 cut(s) 35, 1306
BshTI ACCGGT 1 cut(s) 907
BshVI ATCGAT 1 cut(s) 1078
BsiHKAI GWGCWC 3 cut(s) 12, 688, 907
BsiHKCI CYCGRG 1 cut(s) 797
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 3 cut(s) 8, 335, 535
BslI CCNNNNNNNGG 5 cut(s) 32, 427, 486, 519, 899
BsmAI GTCTC 6 cut(s) 85, 167, 714, 755, 1199, 1437
BsmBI CGTCTC 4 cut(s) 85, 167, 755, 1199
BsmFI GGGAC 3 cut(s) 8, 335, 535
BsnI GGCC 7 cut(s) 302, 431, 772, 866, 1186, 1252, 1391
BsoBI CYCGRG 1 cut(s) 797
Bsp120I GGGCCC 1 cut(s) 300
Bsp1286I GDGCHC 5 cut(s) 12, 304, 396, 688, 907
Bsp13I TCCGGA 2 cut(s) 286, 892
Bsp143I GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
Bsp19I CCATGG 1 cut(s) 742
BspANI GGCC 7 cut(s) 302, 431, 772, 866, 1186, 1252, 1391
BspCNI CTCAG 2 cut(s) 709, 1368
BspDI ATCGAT 1 cut(s) 1078
BspEI TCCGGA 2 cut(s) 286, 892
BspFNI CGCG 7 cut(s) 698, 788, 918, 1154, 1217, 1246, 1410
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 8 cut(s) 37, 302, 323, 324, 523, 659, 891, 1308
BspPI GGATC 5 cut(s) 278, 512, 884, 897, 1083
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 2 cut(s) 35, 1306
BsrBI CCGCTC 1 cut(s) 138
BsrFI RCCGGY 5 cut(s) 607, 772, 814, 907, 1028
BsrI ACTGG 4 cut(s) 295, 431, 904, 1393
BssAI RCCGGY 5 cut(s) 607, 772, 814, 907, 1028
BssECI CCNNGG 4 cut(s) 366, 696, 742, 797
BssMI GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
BssNI GRCGYC 7 cut(s) 36, 153, 549, 585, 1208, 1346, 1503
BssT1I CCWWGG 2 cut(s) 366, 742
Bst4CI ACNGT 4 cut(s) 79, 409, 923, 1318
Bst6I CTCTTC 3 cut(s) 122, 170, 401
BstACI GRCGYC 7 cut(s) 36, 153, 549, 585, 1208, 1346, 1503
BstBAI YACGTR 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 696
BstDEI CTNAG 3 cut(s) 717, 1083, 1376
BstDSI CCRYGG 2 cut(s) 696, 742
BstEII GGTNACC 1 cut(s) 971
BstF5I GGATG 3 cut(s) 223, 239, 864
BstFNI CGCG 7 cut(s) 698, 788, 918, 1154, 1217, 1246, 1410
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 6 cut(s) 38, 254, 607, 788, 1217, 1246
BstKTI GATC 9 cut(s) 286, 507, 573, 672, 892, 1071, 1078, 1082, 1095
BstMAI GTCTC 6 cut(s) 85, 167, 714, 755, 1199, 1437
BstMBI GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
BstMWI GCNNNNNNNGC 2 cut(s) 1178, 1241
BstNSI RCATGY 3 cut(s) 598, 625, 1518
BstPAI GACNNNNGTC 1 cut(s) 875
BstPI GGTNACC 1 cut(s) 971
BstSCI CCNGG 4 cut(s) 660, 796, 797, 1050
BstSLI GKGCMC 2 cut(s) 304, 907
BstUI CGCG 7 cut(s) 698, 788, 918, 1154, 1217, 1246, 1410
BstV1I GCAGC 2 cut(s) 1131, 1323
BstV2I GAAGAC 2 cut(s) 1319, 1349
BstX2I RGATCY 1 cut(s) 889
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 1 cut(s) 889
Bsu15I ATCGAT 1 cut(s) 1078
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 7 cut(s) 302, 431, 772, 866, 1186, 1252, 1391
BsuTUI ATCGAT 1 cut(s) 1078
BtgI CCRYGG 2 cut(s) 696, 742
BtgZI GCGATG 2 cut(s) 187, 912
BtrI CACGTC 5 cut(s) 877, 883, 1036, 1090, 1240
BtsCI GGATG 3 cut(s) 223, 239, 864
BtsI GCAGTG 5 cut(s) 379, 1047, 1164, 1179, 1446
BtsIMutI CAGTG 7 cut(s) 302, 379, 1047, 1164, 1179, 1400, 1446
Cac8I GCNNGC 1 cut(s) 696
CciI TCATGA 1 cut(s) 672
CfoI GCGC 6 cut(s) 38, 254, 607, 788, 1217, 1246
Cfr10I RCCGGY 5 cut(s) 607, 772, 814, 907, 1028
Cfr42I CCGCGG 1 cut(s) 699
Cfr9I CCCGGG 1 cut(s) 797
ClaI ATCGAT 1 cut(s) 1078
CseI GACGC 7 cut(s) 161, 340, 469, 523, 557, 574, 1216
Csp6I GTAC 5 cut(s) 4, 434, 499, 635, 748
CspAI ACCGGT 1 cut(s) 907
CviQI GTAC 5 cut(s) 4, 434, 499, 635, 748
DdeI CTNAG 3 cut(s) 717, 1083, 1376
DinI GGCGCC 1 cut(s) 37
DpnI GATC 9 cut(s) 285, 506, 572, 671, 891, 1070, 1077, 1081, 1094
DpnII GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
DrdI GACNNNNNNGTC 1 cut(s) 554
DriI GACNNNNNGTC 2 cut(s) 446, 584
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 3 cut(s) 122, 170, 401
Eam1105I GACNNNNNGTC 2 cut(s) 446, 584
EarI CTCTTC 3 cut(s) 122, 170, 401
EciI GGCGGA 2 cut(s) 83, 678
Ecl136II GAGCTC 2 cut(s) 10, 686
Eco130I CCWWGG 2 cut(s) 366, 742
Eco147I AGGCCT 1 cut(s) 1186
Eco24I GRGCYC 4 cut(s) 12, 304, 396, 688
Eco47I GGWCC 5 cut(s) 322, 376, 522, 613, 664
Eco53kI GAGCTC 2 cut(s) 10, 686
Eco57I CTGAAG 1 cut(s) 857
Eco88I CYCGRG 1 cut(s) 797
Eco91I GGTNACC 1 cut(s) 971
EcoICRI GAGCTC 2 cut(s) 10, 686
EcoO109I RGGNCCY 2 cut(s) 301, 322
EcoO65I GGTNACC 1 cut(s) 971
EcoT14I CCWWGG 2 cut(s) 366, 742
EcoT38I GRGCYC 4 cut(s) 12, 304, 396, 688
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 366, 742
Esp3I CGTCTC 4 cut(s) 85, 167, 755, 1199
FalI AAGNNNNNCTT 3 cut(s) 101, 133, 1536
FaqI GGGAC 3 cut(s) 8, 335, 535
FauI CCCGC 6 cut(s) 68, 80, 703, 947, 1094, 1403
FbaI TGATCA 2 cut(s) 570, 1092
FblI GTMKAC 3 cut(s) 123, 879, 1349
FokI GGATG 3 cut(s) 210, 226, 871
FriOI GRGCYC 4 cut(s) 12, 304, 396, 688
FspBI CTAG 3 cut(s) 999, 1182, 1493
GlaI GCGC 6 cut(s) 37, 253, 606, 787, 1216, 1245
GsaI CCCAGC 2 cut(s) 728, 935
GsuI CTGGAG 2 cut(s) 508, 1085
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 7 cut(s) 302, 431, 772, 866, 1186, 1252, 1391
HgaI GACGC 7 cut(s) 161, 340, 469, 523, 557, 574, 1216
HhaI GCGC 6 cut(s) 38, 254, 607, 788, 1217, 1246
Hin1I GRCGYC 7 cut(s) 36, 153, 549, 585, 1208, 1346, 1503
Hin6I GCGC 6 cut(s) 36, 252, 605, 786, 1215, 1244
HinP1I GCGC 6 cut(s) 36, 252, 605, 786, 1215, 1244
HincII GTYRAC 5 cut(s) 124, 412, 756, 880, 1350
HindII GTYRAC 5 cut(s) 124, 412, 756, 880, 1350
HinfI GANTC 6 cut(s) 236, 719, 729, 1133, 1360, 1405
HphI GGTGA 1 cut(s) 397
Hpy188I TCNGA 5 cut(s) 235, 468, 714, 950, 1365
Hpy188III TCNNGA 6 cut(s) 287, 667, 673, 893, 1064, 1375
HpyAV CCTTC 3 cut(s) 256, 1007, 1255
HpyCH4III ACNGT 4 cut(s) 79, 409, 923, 1318
HpyCH4V TGCA 6 cut(s) 218, 905, 1005, 1048, 1281, 1451
HpyF10VI GCNNNNNNNGC 2 cut(s) 1178, 1241
HpyF3I CTNAG 3 cut(s) 717, 1083, 1376
Hsp92I GRCGYC 7 cut(s) 36, 153, 549, 585, 1208, 1346, 1503
HspAI GCGC 6 cut(s) 36, 252, 605, 786, 1215, 1244
KasI GGCGCC 1 cut(s) 35
KflI GGGWCCC 1 cut(s) 322
Kpn2I TCCGGA 2 cut(s) 286, 892
Ksp22I TGATCA 2 cut(s) 570, 1092
KspI CCGCGG 1 cut(s) 699
Kzo9I GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 7 cut(s) 15, 117, 143, 489, 683, 691, 1333
Lsp1109I GCAGC 2 cut(s) 1131, 1323
LweI GCATC 3 cut(s) 205, 1035, 1482
MaeI CTAG 3 cut(s) 999, 1182, 1493
MaeIII GTNAC 5 cut(s) 586, 810, 971, 1397, 1429
MalI GATC 9 cut(s) 285, 506, 572, 671, 891, 1070, 1077, 1081, 1094
MbiI CCGCTC 1 cut(s) 138
MboI GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
MboII GAAGA 6 cut(s) 109, 157, 388, 1324, 1354, 1369
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 5 cut(s) 12, 304, 396, 688, 907
MlsI TGGCCA 1 cut(s) 866
MluCI AATT 1 cut(s) 542
MluNI TGGCCA 1 cut(s) 866
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 3 cut(s) 230, 728, 1142
MmeI TCCRAC 1 cut(s) 258
Mox20I TGGCCA 1 cut(s) 866
MroI TCCGGA 2 cut(s) 286, 892
MscI TGGCCA 1 cut(s) 866
MseI TTAA 3 cut(s) 912, 1326, 1554
MslI CAYNNNNRTG 1 cut(s) 593
Msp20I TGGCCA 1 cut(s) 866
MspA1I CMGCKG 2 cut(s) 279, 698
MspR9I CCNGG 4 cut(s) 662, 798, 799, 1052
MvnI CGCG 7 cut(s) 698, 788, 918, 1154, 1217, 1246, 1410
MwoI GCNNNNNNNGC 2 cut(s) 1178, 1241
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 4 cut(s) 662, 798, 799, 1052
NcoI CCATGG 1 cut(s) 742
NdeII GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
NlaIV GGNNCC 8 cut(s) 37, 302, 323, 324, 523, 659, 891, 1308
NmuCI GTSAC 1 cut(s) 586
NspI RCATGY 3 cut(s) 598, 625, 1518
PagI TCATGA 1 cut(s) 672
PceI AGGCCT 1 cut(s) 1186
PciI ACATGT 2 cut(s) 594, 1514
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 6 cut(s) 210, 429, 635, 827, 1119, 1214
PfeI GAWTC 3 cut(s) 729, 1360, 1405
Pfl23II CGTACG 1 cut(s) 498
PflFI GACNNNGTC 1 cut(s) 718
PflMI CCANNNNNTGG 1 cut(s) 486
PinAI ACCGGT 1 cut(s) 907
PleI GAGTC 3 cut(s) 230, 727, 1141
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 3 cut(s) 230, 727, 1141
Ppu21I YACGTR 1 cut(s) 258
PpuMI RGGWCCY 1 cut(s) 322
PscI ACATGT 2 cut(s) 594, 1514
PshAI GACNNNNGTC 1 cut(s) 875
Psp124BI GAGCTC 2 cut(s) 12, 688
Psp1406I AACGTT 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 322
PspEI GGTNACC 1 cut(s) 971
PspFI CCCAGC 2 cut(s) 724, 931
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 8 cut(s) 37, 302, 323, 324, 523, 659, 891, 1308
PspOMI GGGCCC 1 cut(s) 300
PspPPI RGGWCCY 1 cut(s) 322
PsuI RGATCY 1 cut(s) 889
PsyI GACNNNGTC 1 cut(s) 718
RsaI GTAC 5 cut(s) 5, 435, 500, 636, 749
RsaNI GTAC 5 cut(s) 4, 434, 499, 635, 748
RseI CAYNNNNRTG 1 cut(s) 593
SacI GAGCTC 2 cut(s) 12, 688
SacII CCGCGG 1 cut(s) 699
SalI GTCGAC 3 cut(s) 122, 878, 1348
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 3 cut(s) 912, 1326, 1554
Sau3AI GATC 9 cut(s) 283, 504, 570, 669, 889, 1068, 1075, 1079, 1092
SchI GAGTC 3 cut(s) 230, 728, 1142
ScrFI CCNGG 4 cut(s) 662, 798, 799, 1052
SduI GDGCHC 5 cut(s) 12, 304, 396, 688, 907
SfaNI GCATC 3 cut(s) 205, 1035, 1482
SfoI GGCGCC 1 cut(s) 37
Sfr303I CCGCGG 1 cut(s) 699
SgrAI CRCCGGYG 1 cut(s) 607
SgrBI CCGCGG 1 cut(s) 699
SgrDI CGTCGACG 2 cut(s) 878, 1348
SinI GGWCC 5 cut(s) 322, 376, 522, 613, 664
SmaI CCCGGG 1 cut(s) 799
SmiMI CAYNNNNRTG 1 cut(s) 593
Sse9I AATT 1 cut(s) 542
SseBI AGGCCT 1 cut(s) 1186
SspDI GGCGCC 1 cut(s) 35
SspMI CTAG 3 cut(s) 999, 1182, 1493
SstI GAGCTC 2 cut(s) 12, 688
StuI AGGCCT 1 cut(s) 1186
StyD4I CCNGG 4 cut(s) 660, 796, 797, 1050
StyI CCWWGG 2 cut(s) 366, 742
TaaI ACNGT 4 cut(s) 79, 409, 923, 1318
TasI AATT 1 cut(s) 542
TauI GCSGC 8 cut(s) 282, 512, 694, 701, 1157, 1172, 1297, 1391
TfiI GAWTC 3 cut(s) 729, 1360, 1405
Tru1I TTAA 3 cut(s) 912, 1326, 1554
Tru9I TTAA 3 cut(s) 912, 1326, 1554
TscAI CASTG 7 cut(s) 302, 386, 1047, 1164, 1179, 1400, 1453
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 2 cut(s) 1119, 1336
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 3 cut(s) 388, 915, 1022
TspGWI ACGGA 3 cut(s) 149, 836, 1271
TspMI CCCGGG 1 cut(s) 797
TspRI CASTG 7 cut(s) 302, 386, 1047, 1164, 1179, 1400, 1453
Tth111I GACNNNGTC 1 cut(s) 718
Van91I CCANNNNNTGG 1 cut(s) 486
VneI GTGCAC 1 cut(s) 903
VpaK11BI GGWCC 5 cut(s) 322, 376, 522, 613, 664
XapI RAATTY 1 cut(s) 542
XceI RCATGY 3 cut(s) 598, 625, 1518
XmaI CCCGGG 1 cut(s) 797
XmiI GTMKAC 3 cut(s) 123, 879, 1349
XspI CTAG 3 cut(s) 999, 1182, 1493
ZraI GACGTC 2 cut(s) 1347, 1504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.