RLG00000022982

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000677
Physical Location & Seq
Forward (+)
16227037 .. 16231823
4787 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022982

Sequence Viewer

Length: 1701 bp
ATGGAAGAGCTGAAGCCAACCCCCGCCAACTCTTCCCCTCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTGTACGGCGACTGCCCTTCTGTCATATACGAAGACACCACCTACACGTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCATCCGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCATCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCATCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCCGTTCCCTCGTCCTCGACGCCCTCTCTCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACCATGATGCACCCCATCCAAACTCGGACTCCCCCTTCTGCGCCATGTACGAGGAAATGGTGGACAGCGGCGATCCGGACTTCCAGTGGACCCTACCGGAAGACGAGTGGGACCCGATGACCCTGAACTACACTTCCGGCACTACTTCCTCCCCCAAGGGAGTGGTCCACGGCCACAGAGGGCTCTTTATCACCACCATCAACTCTCTGCTTGACTGGGCAGTACCCAGACAGCCGGTCTACCTATGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTTGGTGGGACCAACGTCTGCGTCCGCAAATTCGATGCCCATGTCATCTACGACTTGATCAGACAACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACGAATGTACCGAACAACGAGCCTTTAAGGAACCCGGTCCAGATCATGACTGCCGGAGCTCCGCCGCCCGCGGCAGTTCTGTCTCGGACTGAGTCGCTGGGATTTGTGGTGAGCCATGGGTACGGGTTGACAGAGACGGCAGGACCGGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGATGGTGGCCATGACCCACGTCGATGTGGTGGATCCGGATATGGGTGTGCCGGTTAAACGCGACGGTTCATCGCTGGGGGAGGTGGTTTTCAGAGGCGGGTCTGTGATGTTGGGTTACCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTTGTTTTATACCGGCGACGTGGCAGTGATGCACCCGGACGGGTATCTGGAGATCAAGGATCGATCTAAGGACGTGATTATTAGCGGGGGAGAGAACGTGAGCAGCGTTGAGGTCGAGTCGGTTCTCTACACCAACGCGGCGGTGAACGAGGCGGCAGTTGTGGCTAGGCCTGACGAGTTTTGGGGGGAGACGCCGTGCGCATTTGTGAGCCTGAAAGCTGACGTGGTGCAGAGGCCGACAGAGAAGGAGATAATAGAGTATTGCAGGGGGAAGTTGCCGCATTACATGGTGCCGAAGACAGTGGTGTTTAAGGAGGAGCTACCTAAGACGTCGACGGGGAAGATTCAGAAGTTCGTGCTGAGAGAGATGGCGGCCCAGTGTTACTTGGTTCGCAGGTCGTTGCGAAACGGATACGCGGTACCGAGAGAAACTCCAAGTGCAATCTTTGCAGAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

567

Amino Acids

62.23

Weight (kDa)

6.39

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 401 7.3e-77 AMP-binding enzyme
AMP-binding_C PF13193 451 - 528 3.5e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1574
Acc16I TGCGCA 1 cut(s) 1441
Acc36I ACCTGC 1 cut(s) 1626
Acc65I GGTACC 1 cut(s) 1660
AccB1I GGYRCC 3 cut(s) 263, 1531, 1660
AccB7I CCANNNNNTGG 1 cut(s) 711
AccI GTMKAC 3 cut(s) 351, 675, 1574
AccII CGCG 7 cut(s) 153, 189, 923, 1013, 1143, 1379, 1658
AccIII TCCGGA 2 cut(s) 511, 1117
AclWI GGATC 5 cut(s) 503, 737, 1109, 1122, 1308
AcoI YGGCCR 4 cut(s) 190, 607, 1089, 1221
AcsI RAATTY 1 cut(s) 767
AcuI CTGAAG 2 cut(s) 32, 1082
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 5 cut(s) 264, 381, 810, 1433, 1571
AfaI GTAC 8 cut(s) 80, 233, 485, 660, 725, 861, 974, 1662
AfiI CCNNNNNNNGG 5 cut(s) 123, 260, 711, 744, 1124
AflIII ACRYGT 2 cut(s) 120, 819
AgeI ACCGGT 1 cut(s) 997
AhdI GACNNNNNGTC 2 cut(s) 138, 671
AjiI CACGTC 5 cut(s) 196, 1102, 1261, 1315, 1465
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 554, 586
AluBI AGCT 7 cut(s) 10, 238, 340, 717, 911, 1460, 1561
AluI AGCT 7 cut(s) 10, 238, 340, 717, 911, 1460, 1561
Alw21I GWGCWC 2 cut(s) 240, 913
Alw26I GTCTC 4 cut(s) 205, 939, 980, 1424
AlwI GGATC 5 cut(s) 503, 737, 1109, 1122, 1308
Ama87I CYCGRG 1 cut(s) 1022
Aor13HI TCCGGA 2 cut(s) 511, 1117
AoxI GGCC 7 cut(s) 190, 607, 1089, 1221, 1409, 1475, 1614
ApeKI GCWGC 1 cut(s) 1344
ApoI RAATTY 1 cut(s) 767
AsiGI ACCGGT 1 cut(s) 997
Asp718I GGTACC 1 cut(s) 1660
AspLEI GCGC 5 cut(s) 266, 479, 832, 1013, 1442
AspS9I GGNCC 8 cut(s) 525, 547, 601, 747, 838, 889, 995, 1615
AsuC2I CCSGG 4 cut(s) 887, 1023, 1024, 1277
AsuHPI GGTGA 4 cut(s) 34, 619, 973, 1396
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 7 cut(s) 525, 547, 601, 747, 838, 889, 995
BaeI ACNNNNGTAYC 2 cut(s) 843, 876
BalI TGGCCA 1 cut(s) 1091
BamHI GGATCC 1 cut(s) 1114
BanI GGYRCC 3 cut(s) 263, 1531, 1660
BanII GRGCYC 3 cut(s) 240, 621, 913
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 3 cut(s) 114, 543, 1544
Bbv12I GWGCWC 2 cut(s) 240, 913
BbvI GCAGC 1 cut(s) 1356
BccI CCATC 8 cut(s) 173, 257, 287, 314, 459, 641, 1078, 1603
BceAI ACGGC 9 cut(s) 97, 251, 622, 722, 724, 823, 1005, 1236, 1420
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciVI GTATCC 1 cut(s) 1646
BclI TGATCA 1 cut(s) 795
BcnI CCSGG 4 cut(s) 887, 1023, 1024, 1277
BcoDI GTCTC 4 cut(s) 205, 939, 980, 1424
BfaI CTAG 2 cut(s) 59, 1407
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 1626
BfuI GTATCC 1 cut(s) 1646
Bme1390I CCNGG 4 cut(s) 887, 1023, 1024, 1277
Bme18I GGWCC 7 cut(s) 525, 547, 601, 747, 838, 889, 995
BmeRI GACNNNNNGTC 2 cut(s) 138, 671
BmeT110I CYCGRG 1 cut(s) 1022
BmgBI CACGTC 5 cut(s) 196, 1102, 1261, 1315, 1465
BmgT120I GGNCC 8 cut(s) 525, 547, 601, 747, 838, 889, 995, 1615
BmrFI CCNGG 4 cut(s) 887, 1023, 1024, 1277
BmrI ACTGGG 2 cut(s) 661, 1612
BmsI GCATC 5 cut(s) 134, 189, 433, 763, 1260
BmuI ACTGGG 2 cut(s) 661, 1612
BoxI GACNNNNGTC 2 cut(s) 752, 1100
BpiI GAAGAC 3 cut(s) 114, 543, 1544
BplI GAGNNNNNCTC 2 cut(s) 1657, 1689
BpmI CTGGAG 2 cut(s) 733, 1310
BpuMI CCSGG 4 cut(s) 887, 1023, 1024, 1277
Bsa29I ATCGAT 1 cut(s) 1303
BsaAI YACGTR 1 cut(s) 123
BsaHI GRCGYC 5 cut(s) 264, 381, 810, 1433, 1571
BsaJI CCNNGG 6 cut(s) 175, 591, 604, 921, 967, 1022
BsaWI WCCGGW 4 cut(s) 511, 532, 997, 1117
BsaXI ACNNNNNCTCC 8 cut(s) 185, 215, 449, 479, 1323, 1353, 1421, 1451
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 260, 711, 744, 1124
Bse118I RCCGGY 6 cut(s) 670, 832, 997, 1039, 1132, 1253
Bse1I ACTGG 4 cut(s) 520, 656, 1224, 1618
BseAI TCCGGA 2 cut(s) 511, 1117
BseCI ATCGAT 1 cut(s) 1303
BseDI CCNNGG 6 cut(s) 175, 591, 604, 921, 967, 1022
BseGI GGATG 5 cut(s) 180, 219, 249, 451, 1089
BseLI CCNNNNNNNGG 5 cut(s) 123, 260, 711, 744, 1124
BseMII CTCAG 2 cut(s) 933, 1592
BseNI ACTGG 4 cut(s) 520, 656, 1224, 1618
BseRI GAGGAG 6 cut(s) 160, 305, 308, 332, 410, 1571
BseXI GCAGC 1 cut(s) 1356
BseYI CCCAGC 2 cut(s) 949, 1156
BsgI GTGCAG 1 cut(s) 1490
Bsh1236I CGCG 7 cut(s) 153, 189, 923, 1013, 1143, 1379, 1658
BshFI GGCC 7 cut(s) 192, 609, 1091, 1223, 1411, 1477, 1616
BshNI GGYRCC 3 cut(s) 263, 1531, 1660
BshTI ACCGGT 1 cut(s) 997
BshVI ATCGAT 1 cut(s) 1303
BsiHKAI GWGCWC 2 cut(s) 240, 913
BsiHKCI CYCGRG 1 cut(s) 1022
BsiWI CGTACG 1 cut(s) 723
BslFI GGGAC 2 cut(s) 560, 760
BslI CCNNNNNNNGG 5 cut(s) 123, 260, 711, 744, 1124
BsmAI GTCTC 4 cut(s) 205, 939, 980, 1424
BsmBI CGTCTC 3 cut(s) 205, 980, 1424
BsmFI GGGAC 2 cut(s) 560, 760
BsnI GGCC 7 cut(s) 192, 609, 1091, 1223, 1411, 1477, 1616
BsoBI CYCGRG 1 cut(s) 1022
Bsp1286I GDGCHC 3 cut(s) 240, 621, 913
Bsp13I TCCGGA 2 cut(s) 511, 1117
Bsp143I GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
Bsp19I CCATGG 1 cut(s) 967
BspANI GGCC 7 cut(s) 192, 609, 1091, 1223, 1411, 1477, 1616
BspCNI CTCAG 2 cut(s) 934, 1593
BspDI ATCGAT 1 cut(s) 1303
BspEI TCCGGA 2 cut(s) 511, 1117
BspFNI CGCG 7 cut(s) 153, 189, 923, 1013, 1143, 1379, 1658
BspHI TCATGA 1 cut(s) 897
BspMI ACCTGC 1 cut(s) 1626
BspPI GGATC 5 cut(s) 503, 737, 1109, 1122, 1308
BspT107I GGYRCC 3 cut(s) 263, 1531, 1660
BsrFI RCCGGY 6 cut(s) 670, 832, 997, 1039, 1132, 1253
BsrI ACTGG 4 cut(s) 520, 656, 1224, 1618
BssAI RCCGGY 6 cut(s) 670, 832, 997, 1039, 1132, 1253
BssECI CCNNGG 6 cut(s) 175, 591, 604, 921, 967, 1022
BssMI GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
BssNI GRCGYC 5 cut(s) 264, 381, 810, 1433, 1571
BssT1I CCWWGG 2 cut(s) 591, 967
Bst4CI ACNGT 5 cut(s) 76, 140, 1148, 1241, 1543
Bst6I CTCTTC 3 cut(s) 37, 350, 398
BstACI GRCGYC 5 cut(s) 264, 381, 810, 1433, 1571
BstAPI GCANNNNNTGC 1 cut(s) 1688
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 2 cut(s) 921, 1041
BstDEI CTNAG 4 cut(s) 942, 1308, 1566, 1601
BstDSI CCRYGG 3 cut(s) 604, 921, 967
BstEII GGTNACC 1 cut(s) 1196
BstF5I GGATG 5 cut(s) 180, 219, 249, 451, 1089
BstFNI CGCG 7 cut(s) 153, 189, 923, 1013, 1143, 1379, 1658
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 5 cut(s) 266, 479, 832, 1013, 1442
BstKTI GATC 8 cut(s) 511, 732, 798, 897, 1117, 1296, 1303, 1307
BstMAI GTCTC 4 cut(s) 205, 939, 980, 1424
BstMBI GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
BstMWI GCNNNNNNNGC 3 cut(s) 186, 1403, 1688
BstNSI RCATGY 2 cut(s) 823, 850
BstPAI GACNNNNGTC 2 cut(s) 752, 1100
BstPI GGTNACC 1 cut(s) 1196
BstSCI CCNGG 4 cut(s) 885, 1021, 1022, 1275
BstUI CGCG 7 cut(s) 153, 189, 923, 1013, 1143, 1379, 1658
BstV1I GCAGC 1 cut(s) 1356
BstV2I GAAGAC 3 cut(s) 114, 543, 1544
BstX2I RGATCY 1 cut(s) 1114
BstXI CCANNNNNNTGG 1 cut(s) 598
BstYI RGATCY 1 cut(s) 1114
Bsu15I ATCGAT 1 cut(s) 1303
BsuI GTATCC 1 cut(s) 1646
BsuRI GGCC 7 cut(s) 192, 609, 1091, 1223, 1411, 1477, 1616
BsuTUI ATCGAT 1 cut(s) 1303
BtgI CCRYGG 3 cut(s) 604, 921, 967
BtgZI GCGATG 2 cut(s) 415, 1137
BtrI CACGTC 5 cut(s) 196, 1102, 1261, 1315, 1465
BtsCI GGATG 5 cut(s) 180, 219, 249, 451, 1089
BtsI GCAGTG 1 cut(s) 1272
BtsIMutI CAGTG 4 cut(s) 527, 1272, 1548, 1625
BveI ACCTGC 1 cut(s) 1626
Cac8I GCNNGC 2 cut(s) 921, 1041
CciI TCATGA 1 cut(s) 897
CfoI GCGC 5 cut(s) 266, 479, 832, 1013, 1442
Cfr10I RCCGGY 6 cut(s) 670, 832, 997, 1039, 1132, 1253
Cfr13I GGNCC 8 cut(s) 525, 547, 601, 747, 838, 889, 995, 1615
Cfr42I CCGCGG 1 cut(s) 924
Cfr9I CCCGGG 1 cut(s) 1022
ClaI ATCGAT 1 cut(s) 1303
CseI GACGC 7 cut(s) 142, 195, 389, 694, 748, 799, 1441
Csp6I GTAC 8 cut(s) 79, 232, 484, 659, 724, 860, 973, 1661
CspAI ACCGGT 1 cut(s) 997
CviQI GTAC 8 cut(s) 79, 232, 484, 659, 724, 860, 973, 1661
DdeI CTNAG 4 cut(s) 942, 1308, 1566, 1601
DinI GGCGCC 1 cut(s) 265
DpnI GATC 8 cut(s) 510, 731, 797, 896, 1116, 1295, 1302, 1306
DpnII GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
DriI GACNNNNNGTC 2 cut(s) 138, 671
EaeI YGGCCR 4 cut(s) 190, 607, 1089, 1221
Eam1104I CTCTTC 3 cut(s) 37, 350, 398
Eam1105I GACNNNNNGTC 2 cut(s) 138, 671
EarI CTCTTC 3 cut(s) 37, 350, 398
EciI GGCGGA 2 cut(s) 311, 903
Ecl136II GAGCTC 2 cut(s) 238, 911
Eco130I CCWWGG 2 cut(s) 591, 967
Eco147I AGGCCT 1 cut(s) 1411
Eco24I GRGCYC 3 cut(s) 240, 621, 913
Eco47I GGWCC 7 cut(s) 525, 547, 601, 747, 838, 889, 995
Eco53kI GAGCTC 2 cut(s) 238, 911
Eco57I CTGAAG 2 cut(s) 32, 1082
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 1022
Eco91I GGTNACC 1 cut(s) 1196
EcoICRI GAGCTC 2 cut(s) 238, 911
EcoO109I RGGNCCY 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 1196
EcoT14I CCWWGG 2 cut(s) 591, 967
EcoT38I GRGCYC 3 cut(s) 240, 621, 913
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 591, 967
Esp3I CGTCTC 3 cut(s) 205, 980, 1424
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 2 cut(s) 560, 760
FauI CCCGC 7 cut(s) 31, 232, 296, 308, 928, 1172, 1319
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 3 cut(s) 351, 675, 1574
FokI GGATG 5 cut(s) 167, 206, 236, 438, 1096
FriOI GRGCYC 3 cut(s) 240, 621, 913
FspAI RTGCGCAY 1 cut(s) 1441
FspBI CTAG 2 cut(s) 59, 1407
FspI TGCGCA 1 cut(s) 1441
GlaI GCGC 5 cut(s) 265, 478, 831, 1012, 1441
GsaI CCCAGC 2 cut(s) 953, 1160
GsuI CTGGAG 2 cut(s) 733, 1310
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 7 cut(s) 192, 609, 1091, 1223, 1411, 1477, 1616
HgaI GACGC 7 cut(s) 142, 195, 389, 694, 748, 799, 1441
HhaI GCGC 5 cut(s) 266, 479, 832, 1013, 1442
Hin1I GRCGYC 5 cut(s) 264, 381, 810, 1433, 1571
Hin6I GCGC 5 cut(s) 264, 477, 830, 1011, 1440
HinP1I GCGC 5 cut(s) 264, 477, 830, 1011, 1440
HincII GTYRAC 3 cut(s) 352, 981, 1575
HindII GTYRAC 3 cut(s) 352, 981, 1575
HinfI GANTC 4 cut(s) 464, 944, 1358, 1585
HphI GGTGA 4 cut(s) 34, 619, 973, 1396
Hpy166II GTNNAC 9 cut(s) 79, 352, 499, 525, 604, 676, 981, 1387, 1575
Hpy188I TCNGA 7 cut(s) 185, 463, 693, 800, 939, 1175, 1590
Hpy188III TCNNGA 5 cut(s) 512, 892, 898, 1118, 1289
Hpy8I GTNNAC 9 cut(s) 79, 352, 499, 525, 604, 676, 981, 1387, 1575
HpyAV CCTTC 3 cut(s) 102, 481, 1480
HpyCH4III ACNGT 5 cut(s) 76, 140, 1148, 1241, 1543
HpyCH4IV ACGT 9 cut(s) 122, 195, 753, 1101, 1260, 1314, 1338, 1464, 1571
HpyCH4V TGCA 7 cut(s) 446, 1230, 1273, 1471, 1506, 1682, 1691
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 1403, 1688
HpyF3I CTNAG 4 cut(s) 942, 1308, 1566, 1601
HpySE526I ACGT 9 cut(s) 122, 195, 753, 1101, 1260, 1314, 1338, 1464, 1571
Hsp92I GRCGYC 5 cut(s) 264, 381, 810, 1433, 1571
HspAI GCGC 5 cut(s) 264, 477, 830, 1011, 1440
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 547
Kpn2I TCCGGA 2 cut(s) 511, 1117
KpnI GGTACC 1 cut(s) 1664
KroI GCCGGC 1 cut(s) 1039
KroNI GCCGGC 1 cut(s) 1041
Ksp22I TGATCA 1 cut(s) 795
KspI CCGCGG 1 cut(s) 924
Kzo9I GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
LmnI GCTCC 7 cut(s) 126, 243, 345, 714, 908, 916, 1558
Lsp1109I GCAGC 1 cut(s) 1356
LweI GCATC 5 cut(s) 134, 189, 433, 763, 1260
MaeI CTAG 2 cut(s) 59, 1407
MaeII ACGT 9 cut(s) 122, 195, 753, 1101, 1260, 1314, 1338, 1464, 1571
MaeIII GTNAC 3 cut(s) 811, 1196, 1622
MalI GATC 8 cut(s) 510, 731, 797, 896, 1116, 1295, 1302, 1306
MboI GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
MboII GAAGA 8 cut(s) 17, 24, 119, 337, 385, 548, 1549, 1594
MflI RGATCY 1 cut(s) 1114
MhlI GDGCHC 3 cut(s) 240, 621, 913
MlsI TGGCCA 1 cut(s) 1091
MluCI AATT 1 cut(s) 767
MluNI TGGCCA 1 cut(s) 1091
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 3 cut(s) 458, 953, 1367
MmeI TCCRAC 1 cut(s) 208
Mox20I TGGCCA 1 cut(s) 1091
MroI TCCGGA 2 cut(s) 511, 1117
MroNI GCCGGC 1 cut(s) 1039
MscI TGGCCA 1 cut(s) 1091
MseI TTAA 3 cut(s) 878, 1137, 1551
MslI CAYNNNNRTG 3 cut(s) 254, 818, 1104
Msp20I TGGCCA 1 cut(s) 1091
MspA1I CMGCKG 2 cut(s) 504, 923
MspR9I CCNGG 4 cut(s) 887, 1023, 1024, 1277
MvnI CGCG 7 cut(s) 153, 189, 923, 1013, 1143, 1379, 1658
MwoI GCNNNNNNNGC 3 cut(s) 186, 1403, 1688
NaeI GCCGGC 1 cut(s) 1041
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 4 cut(s) 887, 1023, 1024, 1277
NcoI CCATGG 1 cut(s) 967
NdeII GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
NgoMIV GCCGGC 1 cut(s) 1039
NmeAIII GCCGAG 2 cut(s) 30, 156
NmuCI GTSAC 1 cut(s) 811
NsbI TGCGCA 1 cut(s) 1441
NspI RCATGY 2 cut(s) 823, 850
PagI TCATGA 1 cut(s) 897
PceI AGGCCT 1 cut(s) 1411
PciI ACATGT 1 cut(s) 819
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 860, 1052, 1344
PdiI GCCGGC 1 cut(s) 1041
PfeI GAWTC 1 cut(s) 1585
Pfl23II CGTACG 1 cut(s) 723
PflFI GACNNNGTC 1 cut(s) 943
PflMI CCANNNNNTGG 1 cut(s) 711
PinAI ACCGGT 1 cut(s) 997
PleI GAGTC 3 cut(s) 458, 952, 1366
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 3 cut(s) 458, 952, 1366
Ppu21I YACGTR 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 547
PscI ACATGT 1 cut(s) 819
PshAI GACNNNNGTC 2 cut(s) 752, 1100
Psp124BI GAGCTC 2 cut(s) 240, 913
Psp5II RGGWCCY 1 cut(s) 547
PspCI CACGTG 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 1196
PspFI CCCAGC 2 cut(s) 949, 1156
PspLI CGTACG 1 cut(s) 723
PspPI GGNCC 8 cut(s) 525, 547, 601, 747, 838, 889, 995, 1615
PspPPI RGGWCCY 1 cut(s) 547
PsuI RGATCY 1 cut(s) 1114
PsyI GACNNNGTC 1 cut(s) 943
RsaI GTAC 8 cut(s) 80, 233, 485, 660, 725, 861, 974, 1662
RsaNI GTAC 8 cut(s) 79, 232, 484, 659, 724, 860, 973, 1661
RseI CAYNNNNRTG 3 cut(s) 254, 818, 1104
SacI GAGCTC 2 cut(s) 240, 913
SacII CCGCGG 1 cut(s) 924
SalI GTCGAC 2 cut(s) 350, 1573
SaqAI TTAA 3 cut(s) 878, 1137, 1551
Sau3AI GATC 8 cut(s) 508, 729, 795, 894, 1114, 1293, 1300, 1304
Sau96I GGNCC 8 cut(s) 525, 547, 601, 747, 838, 889, 995, 1615
SchI GAGTC 3 cut(s) 458, 953, 1367
ScrFI CCNGG 4 cut(s) 887, 1023, 1024, 1277
SduI GDGCHC 3 cut(s) 240, 621, 913
SfaNI GCATC 5 cut(s) 134, 189, 433, 763, 1260
SfoI GGCGCC 1 cut(s) 265
Sfr303I CCGCGG 1 cut(s) 924
SgrAI CRCCGGYG 1 cut(s) 832
SgrBI CCGCGG 1 cut(s) 924
SgrDI CGTCGACG 1 cut(s) 1573
SinI GGWCC 7 cut(s) 525, 547, 601, 747, 838, 889, 995
SmaI CCCGGG 1 cut(s) 1024
SmiMI CAYNNNNRTG 3 cut(s) 254, 818, 1104
Sse9I AATT 1 cut(s) 767
SseBI AGGCCT 1 cut(s) 1411
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 59, 1407
SstI GAGCTC 2 cut(s) 240, 913
StuI AGGCCT 1 cut(s) 1411
StyD4I CCNGG 4 cut(s) 885, 1021, 1022, 1275
StyI CCWWGG 2 cut(s) 591, 967
TaaI ACNGT 5 cut(s) 76, 140, 1148, 1241, 1543
TaiI ACGT 9 cut(s) 125, 198, 756, 1104, 1263, 1317, 1341, 1467, 1574
TaqII GACCGA 1 cut(s) 78
TasI AATT 1 cut(s) 767
TfiI GAWTC 1 cut(s) 1585
Tru1I TTAA 3 cut(s) 878, 1137, 1551
Tru9I TTAA 3 cut(s) 878, 1137, 1551
TscAI CASTG 4 cut(s) 527, 1272, 1548, 1625
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 1 cut(s) 1344
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 2 cut(s) 1140, 1247
TspGWI ACGGA 4 cut(s) 193, 353, 1061, 1665
TspMI CCCGGG 1 cut(s) 1022
TspRI CASTG 4 cut(s) 527, 1272, 1548, 1625
Tth111I GACNNNGTC 1 cut(s) 943
Van91I CCANNNNNTGG 1 cut(s) 711
VpaK11BI GGWCC 7 cut(s) 525, 547, 601, 747, 838, 889, 995
XapI RAATTY 1 cut(s) 767
XceI RCATGY 2 cut(s) 823, 850
XmaI CCCGGG 1 cut(s) 1022
XmiI GTMKAC 3 cut(s) 351, 675, 1574
XspI CTAG 2 cut(s) 59, 1407
ZraI GACGTC 1 cut(s) 1572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.