Rroxscaffold_6G00394740

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000677
Physical Location & Seq
Forward (+)
15566250 .. 15567913
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394740.1

Sequence Viewer

Length: 1491 bp
ATGGACCAGCTGAAACCTAGTTCTGCAAACCTCTGCCCTCAAACTCCCCTGGACTTCTTAGACCGAGCAGCGATCGTCTACGGTGACTGCCCCTCCATCATCTACAACTCCACCACCACCTACACGTGGTCCCAAACCCACCGTCGATGTCTCCAGCTGGCTTGCTCCATCTCCACCATCGTCAAGAAATCCCAGGTCGTCTCCGTGGTGTCCCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTCCCGATGTCGGGAGCCGTCCTCAACAACATCAACACCCGCCTCGACGCCCACACCGTCTCCGTGCTCCTCCAACACAGCGAATCCAAGCTCGTCTTCGTCGACTTCCACTCCCTCAGCCTCGTCCTCCAAGCCCTCTCCTTGTTCCCCTCTCACATCCCCACCCCACTTCTCGTCCTCATCAACGATCATCATCAACCCTCCTCAACCTCCACCGTTGACTTCATAGCGGACACGTACGACAACATGGTGGCCAAAGGCGATCCCGGGTTCGTTTGGGTCCGGCCGGCGAGCGAGTGGGACCCAATGGTGCTGAACTACACTTCAGGGACGACGTCAGCTCCGAAAGGTGCGGTCCACTGCCACAGAGCTCTCTTCAACAAACACAGAGTGACTCACATGTGCGCCGCGCCTGTCGTTCTCAACATGCTGGTCAACCACCCGAGAACCGACCCGCTTCCGAACCCGGTTTGTATCTTAACAGGTGGAGCTCCGCCTCCTTCGAGCGTGCTGCTCCGGGCCGAGTCGCTCGGGTTTGTTGTGAGTCACGGGTACGGTTTGACTGAAACGGCAGGGGTGGTGGTGTCGTGTGCCTGGAAGCCGGAGTGGAATAAGTTTCCGGTGACGGAGAAGGCGGGGTTGAAGGCGAGGCAAGGAGTGAGGACGGTTGGAATGACCGACGCGGATGTGGTGGATCCGGATTCGGGTTTGAGCGTGAAACAAAACGGGTCGGATATTGGAGAAATAGTTCTGCGAGGCGGGTGCGTCATGTTGGGATACCTCAAAGACCCGGAAACGACGAGCAAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACAAACGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTAAGGCCGAGGCCGACGGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACATCAACGACGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

496

Amino Acids

54.06

Weight (kDa)

6.94

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 210 3.8e-30 AMP-binding enzyme
AMP-binding PF00501 208 - 344 4.8e-22 AMP-binding enzyme
AMP-binding_C PF13193 395 - 472 5.9e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 587, 1095, 1406
AccB1I GGYRCC 1 cut(s) 1363
AccI GTMKAC 4 cut(s) 78, 351, 1406, 1460
AccII CGCG 4 cut(s) 659, 932, 1214, 1274
AccIII TCCGGA 1 cut(s) 946
AclWI GGATC 5 cut(s) 506, 938, 951, 1131, 1140
AcoI YGGCCR 3 cut(s) 501, 533, 1227
AcsI RAATTY 1 cut(s) 1354
AcuI CTGAAG 1 cut(s) 558
AcvI CACGTG 1 cut(s) 126
AcyI GRCGYC 5 cut(s) 297, 584, 1092, 1265, 1403
AdeI CACNNNGTG 1 cut(s) 640
AfaI GTAC 3 cut(s) 233, 488, 803
AfiI CCNNNNNNNGG 4 cut(s) 126, 259, 260, 953
AflII CTTAAG 1 cut(s) 1171
AflIII ACRYGT 3 cut(s) 123, 483, 648
AgsI TTSAA 3 cut(s) 628, 892, 1384
AjiI CACGTC 1 cut(s) 1147
AjnI CCWGG 3 cut(s) 48, 192, 842
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 981, 1013
AluBI AGCT 9 cut(s) 10, 157, 340, 590, 620, 740, 1292, 1348, 1393
AluI AGCT 9 cut(s) 10, 157, 340, 590, 620, 740, 1292, 1348, 1393
Alw21I GWGCWC 3 cut(s) 318, 622, 742
Alw26I GTCTC 4 cut(s) 155, 205, 313, 1256
AlwI GGATC 5 cut(s) 506, 938, 951, 1131, 1140
Ama87I CYCGRG 3 cut(s) 515, 691, 779
Aor13HI TCCGGA 1 cut(s) 946
AoxI GGCC 6 cut(s) 501, 533, 768, 1227, 1301, 1307
ApeKI GCWGC 5 cut(s) 68, 760, 1176, 1348, 1393
ApoI RAATTY 1 cut(s) 1354
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
Asp700I GAANNNNTTC 1 cut(s) 996
AspLEI GCGC 3 cut(s) 656, 661, 1274
AspS9I GGNCC 7 cut(s) 4, 129, 529, 550, 604, 768, 1476
AsuC2I CCSGG 6 cut(s) 516, 517, 716, 767, 1040, 1109
AsuHPI GGTGA 3 cut(s) 95, 883, 1228
AvaI CYCGRG 3 cut(s) 515, 691, 779
AvaII GGWCC 6 cut(s) 4, 129, 529, 550, 604, 1476
BalI TGGCCA 1 cut(s) 503
BamHI GGATCC 1 cut(s) 943
BanI GGYRCC 1 cut(s) 1363
BanII GRGCYC 2 cut(s) 622, 742
BbrPI CACGTG 1 cut(s) 126
BbsI GAAGAC 3 cut(s) 337, 1376, 1406
Bbv12I GWGCWC 3 cut(s) 318, 622, 742
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 5 cut(s) 80, 747, 1188, 1335, 1380
BccI CCATC 3 cut(s) 104, 176, 185
BceAI ACGGC 4 cut(s) 251, 834, 1214, 1252
BcgI CGANNNNNNTGC 4 cut(s) 742, 776, 993, 1027
BciT130I CCWGG 3 cut(s) 50, 194, 844
BciVI GTATCC 1 cut(s) 1019
BclI TGATCA 1 cut(s) 1149
BcnI CCSGG 6 cut(s) 516, 517, 716, 767, 1040, 1109
BcoDI GTCTC 4 cut(s) 155, 205, 313, 1256
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 1336
BfrI CTTAAG 1 cut(s) 1171
BfuI GTATCC 1 cut(s) 1019
BglI GCCNNNNNGGC 1 cut(s) 1235
BisI GCNGC 8 cut(s) 69, 657, 761, 1177, 1212, 1227, 1349, 1394
BlpI GCTNAGC 1 cut(s) 1239
BlsI GCNGC 8 cut(s) 70, 658, 762, 1178, 1213, 1228, 1350, 1395
Bme1390I CCNGG 9 cut(s) 50, 194, 516, 517, 716, 767, 844, 1040, 1109
Bme18I GGWCC 6 cut(s) 4, 129, 529, 550, 604, 1476
BmeT110I CYCGRG 3 cut(s) 515, 691, 779
BmgBI CACGTC 1 cut(s) 1147
BmgT120I GGNCC 7 cut(s) 4, 129, 529, 550, 604, 768, 1476
BmiI GGNNCC 7 cut(s) 131, 265, 530, 551, 552, 945, 1365
BmrFI CCNGG 9 cut(s) 50, 194, 516, 517, 716, 767, 844, 1040, 1109
BpiI GAAGAC 3 cut(s) 337, 1376, 1406
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 2 cut(s) 137, 1142
Bpu10I CCTNAGC 1 cut(s) 365
Bpu1102I GCTNAGC 1 cut(s) 1239
BpuMI CCSGG 6 cut(s) 516, 517, 716, 767, 1040, 1109
Bsa29I ATCGAT 1 cut(s) 1135
BsaAI YACGTR 2 cut(s) 126, 486
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 5 cut(s) 297, 584, 1092, 1265, 1403
BsaJI CCNNGG 8 cut(s) 48, 192, 204, 515, 1140, 1212, 1230, 1304
BsaWI WCCGGW 2 cut(s) 868, 946
Bsc4I CCNNNNNNNGG 4 cut(s) 126, 259, 260, 953
Bse118I RCCGGY 2 cut(s) 535, 1085
Bse1I ACTGG 1 cut(s) 1479
Bse8I GATNNNNATC 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 946
BseBI CCWGG 3 cut(s) 50, 194, 844
BseCI ATCGAT 1 cut(s) 1135
BseDI CCNNGG 8 cut(s) 48, 192, 204, 515, 1140, 1212, 1230, 1304
BseGI GGATG 2 cut(s) 405, 940
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 4 cut(s) 126, 259, 260, 953
BseMII CTCAG 2 cut(s) 379, 1424
BseNI ACTGG 1 cut(s) 1479
BseRI GAGGAG 2 cut(s) 308, 442
BseX3I CGGCCG 2 cut(s) 533, 1227
BseXI GCAGC 5 cut(s) 80, 747, 1188, 1335, 1380
Bsh1236I CGCG 4 cut(s) 659, 932, 1214, 1274
Bsh1285I CGRYCG 3 cut(s) 75, 536, 1230
BshFI GGCC 6 cut(s) 503, 535, 770, 1229, 1303, 1309
BshNI GGYRCC 1 cut(s) 1363
BshVI ATCGAT 1 cut(s) 1135
BsiEI CGRYCG 3 cut(s) 75, 536, 1230
BsiHKAI GWGCWC 3 cut(s) 318, 622, 742
BsiHKCI CYCGRG 3 cut(s) 515, 691, 779
BsiWI CGTACG 1 cut(s) 486
BslFI GGGAC 5 cut(s) 115, 196, 236, 563, 592
BslI CCNNNNNNNGG 4 cut(s) 126, 259, 260, 953
BsmAI GTCTC 4 cut(s) 155, 205, 313, 1256
BsmBI CGTCTC 3 cut(s) 205, 313, 1256
BsmFI GGGAC 5 cut(s) 115, 196, 236, 563, 592
BsnI GGCC 6 cut(s) 503, 535, 770, 1229, 1303, 1309
BsoBI CYCGRG 3 cut(s) 515, 691, 779
Bsp1286I GDGCHC 3 cut(s) 318, 622, 742
Bsp13I TCCGGA 1 cut(s) 946
Bsp143I GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
Bsp1720I GCTNAGC 1 cut(s) 1239
BspANI GGCC 6 cut(s) 503, 535, 770, 1229, 1303, 1309
BspCNI CTCAG 2 cut(s) 378, 1425
BspDI ATCGAT 1 cut(s) 1135
BspEI TCCGGA 1 cut(s) 946
BspFNI CGCG 4 cut(s) 659, 932, 1214, 1274
BspLI GGNNCC 7 cut(s) 131, 265, 530, 551, 552, 945, 1365
BspPI GGATC 5 cut(s) 506, 938, 951, 1131, 1140
BspT107I GGYRCC 1 cut(s) 1363
BspTI CTTAAG 1 cut(s) 1171
BsrFI RCCGGY 2 cut(s) 535, 1085
BsrI ACTGG 1 cut(s) 1479
BssAI RCCGGY 2 cut(s) 535, 1085
BssECI CCNNGG 8 cut(s) 48, 192, 204, 515, 1140, 1212, 1230, 1304
BssMI GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
BssNI GRCGYC 5 cut(s) 297, 584, 1092, 1265, 1403
BssT1I CCWWGG 1 cut(s) 1140
Bst2UI CCWGG 3 cut(s) 50, 194, 844
Bst4CI ACNGT 7 cut(s) 83, 143, 307, 466, 806, 916, 1375
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 5 cut(s) 297, 584, 1092, 1265, 1403
BstAFI CTTAAG 1 cut(s) 1171
BstBAI YACGTR 2 cut(s) 126, 486
BstC8I GCNNGC 5 cut(s) 159, 163, 537, 541, 758
BstDEI CTNAG 4 cut(s) 58, 365, 1239, 1433
BstDSI CCRYGG 3 cut(s) 204, 1212, 1230
BstF5I GGATG 2 cut(s) 405, 940
BstFNI CGCG 4 cut(s) 659, 932, 1214, 1274
BstHHI GCGC 3 cut(s) 656, 661, 1274
BstKTI GATC 7 cut(s) 75, 439, 514, 946, 1135, 1139, 1152
BstMAI GTCTC 4 cut(s) 155, 205, 313, 1256
BstMBI GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
BstMCI CGRYCG 3 cut(s) 75, 536, 1230
BstMWI GCNNNNNNNGC 1 cut(s) 1235
BstNI CCWGG 3 cut(s) 50, 194, 844
BstNSI RCATGY 2 cut(s) 652, 679
BstSCI CCNGG 9 cut(s) 48, 192, 514, 515, 714, 765, 842, 1038, 1107
BstSFI CTRYAG 1 cut(s) 1336
BstUI CGCG 4 cut(s) 659, 932, 1214, 1274
BstV1I GCAGC 5 cut(s) 80, 747, 1188, 1335, 1380
BstV2I GAAGAC 3 cut(s) 337, 1376, 1406
BstX2I RGATCY 1 cut(s) 943
BstYI RGATCY 1 cut(s) 943
BstZI CGGCCG 2 cut(s) 533, 1227
Bsu15I ATCGAT 1 cut(s) 1135
BsuI GTATCC 1 cut(s) 1019
BsuRI GGCC 6 cut(s) 503, 535, 770, 1229, 1303, 1309
BsuTUI ATCGAT 1 cut(s) 1135
BtgI CCRYGG 3 cut(s) 204, 1212, 1230
BtrI CACGTC 1 cut(s) 1147
BtsCI GGATG 2 cut(s) 405, 940
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 1 cut(s) 607
Cac8I GCNNGC 5 cut(s) 159, 163, 537, 541, 758
CfoI GCGC 3 cut(s) 656, 661, 1274
Cfr10I RCCGGY 2 cut(s) 535, 1085
Cfr13I GGNCC 7 cut(s) 4, 129, 529, 550, 604, 768, 1476
Cfr42I CCGCGG 1 cut(s) 1215
Cfr9I CCCGGG 1 cut(s) 515
ClaI ATCGAT 1 cut(s) 1135
CseI GACGC 4 cut(s) 305, 938, 1003, 1273
Csp6I GTAC 3 cut(s) 232, 487, 802
CviAII CATG 6 cut(s) 229, 496, 649, 676, 1018, 1360
CviQI GTAC 3 cut(s) 232, 487, 802
DdeI CTNAG 4 cut(s) 58, 365, 1239, 1433
DpnI GATC 7 cut(s) 74, 438, 513, 945, 1134, 1138, 1151
DpnII GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
DraIII CACNNNGTG 1 cut(s) 640
EaeI YGGCCR 3 cut(s) 501, 533, 1227
EagI CGGCCG 2 cut(s) 533, 1227
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
EciI GGCGGA 1 cut(s) 732
Ecl136II GAGCTC 2 cut(s) 620, 740
EclXI CGGCCG 2 cut(s) 533, 1227
Eco130I CCWWGG 1 cut(s) 1140
Eco24I GRGCYC 2 cut(s) 622, 742
Eco47I GGWCC 6 cut(s) 4, 129, 529, 550, 604, 1476
Eco52I CGGCCG 2 cut(s) 533, 1227
Eco53kI GAGCTC 2 cut(s) 620, 740
Eco57I CTGAAG 1 cut(s) 558
Eco72I CACGTG 1 cut(s) 126
Eco88I CYCGRG 3 cut(s) 515, 691, 779
EcoICRI GAGCTC 2 cut(s) 620, 740
EcoO109I RGGNCCY 1 cut(s) 550
EcoRII CCWGG 3 cut(s) 48, 192, 842
EcoT14I CCWWGG 1 cut(s) 1140
EcoT38I GRGCYC 2 cut(s) 622, 742
ErhI CCWWGG 1 cut(s) 1140
Esp3I CGTCTC 3 cut(s) 205, 313, 1256
FaeI CATG 6 cut(s) 232, 499, 652, 679, 1021, 1363
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 5 cut(s) 115, 196, 236, 563, 592
FatI CATG 6 cut(s) 228, 495, 648, 675, 1017, 1359
FauI CCCGC 6 cut(s) 232, 296, 711, 877, 1001, 1151
FbaI TGATCA 1 cut(s) 1149
FblI GTMKAC 4 cut(s) 78, 351, 1406, 1460
Fnu4HI GCNGC 8 cut(s) 69, 657, 761, 1177, 1212, 1227, 1349, 1394
FokI GGATG 2 cut(s) 392, 947
FriOI GRGCYC 2 cut(s) 622, 742
Fsp4HI GCNGC 8 cut(s) 69, 657, 761, 1177, 1212, 1227, 1349, 1394
FspBI CTAG 1 cut(s) 18
GlaI GCGC 3 cut(s) 655, 660, 1273
GluI GCNGC 8 cut(s) 69, 657, 761, 1177, 1212, 1227, 1349, 1394
GsuI CTGGAG 2 cut(s) 137, 1142
HaeIII GGCC 6 cut(s) 503, 535, 770, 1229, 1303, 1309
HgaI GACGC 4 cut(s) 305, 938, 1003, 1273
HhaI GCGC 3 cut(s) 656, 661, 1274
Hin1I GRCGYC 5 cut(s) 297, 584, 1092, 1265, 1403
Hin1II CATG 6 cut(s) 232, 499, 652, 679, 1021, 1363
Hin6I GCGC 3 cut(s) 654, 659, 1272
HinP1I GCGC 3 cut(s) 654, 659, 1272
HincII GTYRAC 4 cut(s) 352, 469, 685, 1407
HindII GTYRAC 4 cut(s) 352, 469, 685, 1407
HinfI GANTC 7 cut(s) 332, 643, 773, 793, 950, 1190, 1417
HphI GGTGA 3 cut(s) 95, 883, 1228
Hpy166II GTNNAC 8 cut(s) 79, 352, 469, 607, 685, 1219, 1407, 1461
Hpy188I TCNGA 4 cut(s) 594, 711, 982, 1422
Hpy188III TCNNGA 5 cut(s) 184, 253, 261, 947, 1121
Hpy8I GTNNAC 8 cut(s) 79, 352, 469, 607, 685, 1219, 1407, 1461
HpyAV CCTTC 4 cut(s) 759, 874, 886, 1312
HpyCH4III ACNGT 7 cut(s) 83, 143, 307, 466, 806, 916, 1375
HpyCH4IV ACGT 7 cut(s) 125, 485, 584, 1092, 1146, 1296, 1403
HpyCH4V TGCA 2 cut(s) 26, 1105
HpyF10VI GCNNNNNNNGC 1 cut(s) 1235
HpyF3I CTNAG 4 cut(s) 58, 365, 1239, 1433
HpySE526I ACGT 7 cut(s) 125, 485, 584, 1092, 1146, 1296, 1403
Hsp92I GRCGYC 5 cut(s) 297, 584, 1092, 1265, 1403
Hsp92II CATG 6 cut(s) 232, 499, 652, 679, 1021, 1363
HspAI GCGC 3 cut(s) 654, 659, 1272
KflI GGGWCCC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 946
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 1 cut(s) 1149
KspI CCGCGG 1 cut(s) 1215
Kzo9I GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
LmnI GCTCC 7 cut(s) 170, 263, 321, 595, 737, 745, 768
Lsp1109I GCAGC 5 cut(s) 80, 747, 1188, 1335, 1380
MaeI CTAG 1 cut(s) 18
MaeII ACGT 7 cut(s) 125, 485, 584, 1092, 1146, 1296, 1403
MaeIII GTNAC 4 cut(s) 83, 640, 794, 871
MalI GATC 7 cut(s) 74, 438, 513, 945, 1134, 1138, 1151
MboI GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
MboII GAAGA 5 cut(s) 337, 616, 1381, 1411, 1426
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 3 cut(s) 318, 622, 742
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 1 cut(s) 1354
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 4 cut(s) 637, 782, 802, 1199
MmeI TCCRAC 3 cut(s) 346, 898, 960
Mox20I TGGCCA 1 cut(s) 503
MroI TCCGGA 1 cut(s) 946
MroNI GCCGGC 1 cut(s) 535
MroXI GAANNNNTTC 1 cut(s) 996
MscI TGGCCA 1 cut(s) 503
MseI TTAA 2 cut(s) 728, 1172
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 3 cut(s) 10, 157, 1214
MspCI CTTAAG 1 cut(s) 1171
MspR9I CCNGG 9 cut(s) 50, 194, 516, 517, 716, 767, 844, 1040, 1109
MteI GCGCNGCGC 1 cut(s) 657
MvaI CCWGG 3 cut(s) 50, 194, 844
MvnI CGCG 4 cut(s) 659, 932, 1214, 1274
MwoI GCNNNNNNNGC 1 cut(s) 1235
NaeI GCCGGC 1 cut(s) 537
NciI CCSGG 6 cut(s) 516, 517, 716, 767, 1040, 1109
NdeII GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
NgoMIV GCCGGC 1 cut(s) 535
NlaIII CATG 6 cut(s) 232, 499, 652, 679, 1021, 1363
NlaIV GGNNCC 7 cut(s) 131, 265, 530, 551, 552, 945, 1365
NmeAIII GCCGAG 2 cut(s) 796, 1329
NmuCI GTSAC 4 cut(s) 83, 640, 794, 871
NspI RCATGY 2 cut(s) 652, 679
PciI ACATGT 1 cut(s) 648
PcsI WCGNNNNNNNCGW 5 cut(s) 303, 348, 590, 1227, 1271
PdiI GCCGGC 1 cut(s) 537
PdmI GAANNNNTTC 1 cut(s) 996
PfeI GAWTC 3 cut(s) 332, 950, 1417
Pfl23II CGTACG 1 cut(s) 486
PflFI GACNNNGTC 2 cut(s) 583, 1474
PkrI GCNGC 8 cut(s) 70, 658, 762, 1178, 1213, 1228, 1350, 1395
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 4 cut(s) 637, 781, 801, 1198
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
PpsI GAGTC 4 cut(s) 637, 781, 801, 1198
Ppu21I YACGTR 2 cut(s) 126, 486
PpuMI RGGWCCY 1 cut(s) 550
PscI ACATGT 1 cut(s) 648
Psp124BI GAGCTC 2 cut(s) 622, 742
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 3 cut(s) 48, 192, 842
PspCI CACGTG 1 cut(s) 126
PspGI CCWGG 3 cut(s) 48, 192, 842
PspLI CGTACG 1 cut(s) 486
PspN4I GGNNCC 7 cut(s) 131, 265, 530, 551, 552, 945, 1365
PspPI GGNCC 7 cut(s) 4, 129, 529, 550, 604, 768, 1476
PspPPI RGGWCCY 1 cut(s) 550
PsuI RGATCY 1 cut(s) 943
PsyI GACNNNGTC 2 cut(s) 583, 1474
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 3 cut(s) 233, 488, 803
RsaNI GTAC 3 cut(s) 232, 487, 802
SacI GAGCTC 2 cut(s) 622, 742
SacII CCGCGG 1 cut(s) 1215
SalI GTCGAC 2 cut(s) 350, 1405
SaqAI TTAA 2 cut(s) 728, 1172
SatI GCNGC 8 cut(s) 69, 657, 761, 1177, 1212, 1227, 1349, 1394
Sau3AI GATC 7 cut(s) 72, 436, 511, 943, 1132, 1136, 1149
Sau96I GGNCC 7 cut(s) 4, 129, 529, 550, 604, 768, 1476
SchI GAGTC 4 cut(s) 637, 782, 802, 1199
ScrFI CCNGG 9 cut(s) 50, 194, 516, 517, 716, 767, 844, 1040, 1109
SduI GDGCHC 3 cut(s) 318, 622, 742
SfcI CTRYAG 1 cut(s) 1336
Sfr303I CCGCGG 1 cut(s) 1215
SgrAI CRCCGGYG 1 cut(s) 1085
SgrBI CCGCGG 1 cut(s) 1215
SgrDI CGTCGACG 1 cut(s) 1405
SinI GGWCC 6 cut(s) 4, 129, 529, 550, 604, 1476
SmaI CCCGGG 1 cut(s) 517
SmlI CTYRAG 1 cut(s) 1171
SmoI CTYRAG 1 cut(s) 1171
Sse9I AATT 1 cut(s) 1354
SspI AATATT 1 cut(s) 1453
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 2 cut(s) 622, 742
StyD4I CCNGG 9 cut(s) 48, 192, 514, 515, 714, 765, 842, 1038, 1107
StyI CCWWGG 1 cut(s) 1140
TaaI ACNGT 7 cut(s) 83, 143, 307, 466, 806, 916, 1375
TaiI ACGT 7 cut(s) 128, 488, 587, 1095, 1149, 1299, 1406
TaqI TCGA 7 cut(s) 145, 294, 351, 752, 1135, 1188, 1406
TaqII GACCGA 2 cut(s) 78, 941
TasI AATT 1 cut(s) 1354
TauI GCSGC 3 cut(s) 659, 1214, 1229
TfiI GAWTC 3 cut(s) 332, 950, 1417
Tru1I TTAA 2 cut(s) 728, 1172
Tru9I TTAA 2 cut(s) 728, 1172
TscAI CASTG 1 cut(s) 614
TseFI GTSAC 4 cut(s) 83, 640, 794, 871
TseI GCWGC 5 cut(s) 68, 760, 1176, 1348, 1393
Tsp45I GTSAC 4 cut(s) 83, 640, 794, 871
TspDTI ATGAA 2 cut(s) 463, 1348
TspGWI ACGGA 4 cut(s) 193, 301, 890, 1328
TspMI CCCGGG 1 cut(s) 515
TspRI CASTG 1 cut(s) 614
Tth111I GACNNNGTC 2 cut(s) 583, 1474
Vha464I CTTAAG 1 cut(s) 1171
VpaK11BI GGWCC 6 cut(s) 4, 129, 529, 550, 604, 1476
XapI RAATTY 1 cut(s) 1354
XceI RCATGY 2 cut(s) 652, 679
XmaI CCCGGG 1 cut(s) 515
XmiI GTMKAC 4 cut(s) 78, 351, 1406, 1460
XmnI GAANNNNTTC 1 cut(s) 996
XspI CTAG 1 cut(s) 18
ZraI GACGTC 3 cut(s) 585, 1093, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.