Rw0G009700

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00384
OG0000677
Physical Location & Seq
Reverse (-)
23516 .. 25314
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G009700.1

Sequence Viewer

Length: 1617 bp
ATGGACCAGCTGAAACCTAGTTCTGCAAACCTCTGCCCTCAAACTCCCCTGGACTTCTTAGACCGAGCAGCGATCGTCTACGGCGACTGCCCCTCCATCATCTACAACTCCACCACCACCTACACCTGGTCCCAAACCCACCGTCGATGTCTCCAGCTGGCTTCCTCCATCTCCACCCTCGTCAAGAAATCCCAGGTCGTCTCCGTGGTGTCCCCCAACACTCCCGCCATGTACGAACTCCACTTCGCTGTCCCCATGTCGGGAGCCGTCCTCAACAACATCAACACCCGCCTCGACGCCCACACAGTCTCCGTGCTCCTCCAACACAGCGAATCCAAGCTCGTCTTCGTCGACTTCCTCTCCCTCAACCTCGTCCTCCAAGCCCTCTCCTTGTTCCCCTCTCACATCCCCACCCCACCTCTCGTCCTCATCAACGATCATCATCAACCCTCCTCAACCTCCAGTACCGTTGACTTCATAGCGGACACGTACGACAACTTGGTGGCCAAAGGCGATCCCGGGTTCGTTTGGGTCCGGCCGGCAAGCGAGTGGGACCCAATGGTGCTGAACTACACTTCAGGGACGACGTCAGCTCCGAAAGGTGCGGTCCACTGCCACAGAGCTCTCTACAACGTGAGTCTGGTTTCAATCATCGACTGGTCGGTTCCTAAAAGTTGTGTTTATCTGTGGACCCTACCGATGTTCCATGCAAATGGGTGGTGCTTCCCGTGGGCCATGGCCGCCGTGGGGGGGACCAACATCTGCCTTCGCAAGTTCGACGGTCCTATAATCTACGATCTGATCCAGAAACACAGAGTGACTCACATGTGCGCCGCGCCTGTCGTTCTCAACATGCTGGGCGGAGCTCCGCCTCCTTCGAGCGTGCTGCTCCGGGCCGAATCGCTCGGGTTTGTTGTGAGTCACGGGTACGGGTTGACTGAAACGGCCGGGGTGGTGGTGTCGTGTGCCTGGAAGCCGGAGTGGAATGAGTTTCCGGCGACGGAGAAGGCGAGGTTGAAGGCGAGGCAAGGAGTGAGGACGGTTGGAATGACCGACGCGGATGTGGTGGATCCGGATTCGGGTTTGAGAGTGAAACGAAACGGGTCGGATATTGGAGAAATAGTCATACGAGGCGGGTGCGTCATGTTGGGCTACCTCAAAGACCCGGAAACGACGAGCAAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTGGAGATAAAGGATCGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACGCCGCGGTGAACGAGGCGGCCGTGGTGGCTAAGCCTGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAACCTGACGTCAGGCCGAGGCCGACGGAGAAGGAGATAATAGAGTTCTGTAGAGGAAAAATGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGTTTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACATCAACGACGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

538

Amino Acids

58.99

Weight (kDa)

7.2

Isoelectric Point (pI)

36.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 386 1.7e-60 AMP-binding enzyme
AMP-binding_C PF13193 437 - 514 5.4e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 4 cut(s) 590, 1221, 1425, 1532
AccB1I GGYRCC 1 cut(s) 1489
AccI GTMKAC 4 cut(s) 78, 351, 1532, 1586
AccII CGCG 4 cut(s) 836, 1058, 1340, 1400
AccIII TCCGGA 1 cut(s) 1072
AclWI GGATC 6 cut(s) 509, 796, 1064, 1077, 1257, 1266
AcoI YGGCCR 5 cut(s) 504, 536, 738, 945, 1353
AcsI RAATTY 1 cut(s) 1480
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 6 cut(s) 297, 587, 1218, 1391, 1422, 1529
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 4 cut(s) 233, 466, 491, 929
AfiI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1079
AflII CTTAAG 1 cut(s) 1297
AflIII ACRYGT 2 cut(s) 486, 825
AgsI TTSAA 3 cut(s) 648, 1018, 1510
AjiI CACGTC 1 cut(s) 1273
AjnI CCWGG 4 cut(s) 48, 125, 192, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 7 cut(s) 10, 157, 340, 593, 623, 866, 1519
AluI AGCT 7 cut(s) 10, 157, 340, 593, 623, 866, 1519
Alw21I GWGCWC 3 cut(s) 318, 625, 868
Alw26I GTCTC 4 cut(s) 155, 205, 313, 1382
AlwI GGATC 6 cut(s) 509, 796, 1064, 1077, 1257, 1266
Ama87I CYCGRG 2 cut(s) 518, 905
Aor13HI TCCGGA 1 cut(s) 1072
AoxI GGCC 9 cut(s) 504, 536, 732, 738, 894, 945, 1353, 1427, 1433
ApeKI GCWGC 4 cut(s) 68, 886, 1302, 1519
ApoI RAATTY 1 cut(s) 1480
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspLEI GCGC 3 cut(s) 833, 838, 1400
AsuC2I CCSGG 6 cut(s) 519, 520, 893, 949, 1166, 1235
AsuHPI GGTGA 1 cut(s) 1354
AvaI CYCGRG 2 cut(s) 518, 905
AvaII GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1602
BalI TGGCCA 1 cut(s) 506
BamHI GGATCC 1 cut(s) 1069
BanI GGYRCC 1 cut(s) 1489
BanII GRGCYC 2 cut(s) 625, 868
BbsI GAAGAC 3 cut(s) 337, 1502, 1532
Bbv12I GWGCWC 3 cut(s) 318, 625, 868
BbvI GCAGC 4 cut(s) 80, 873, 1314, 1506
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 6 cut(s) 97, 251, 728, 960, 1340, 1378
BcgI CGANNNNNNTGC 4 cut(s) 868, 902, 1119, 1153
BciT130I CCWGG 4 cut(s) 50, 127, 194, 970
BclI TGATCA 1 cut(s) 1275
BcnI CCSGG 6 cut(s) 519, 520, 893, 949, 1166, 1235
BcoDI GTCTC 4 cut(s) 155, 205, 313, 1382
BfaI CTAG 1 cut(s) 18
BfmI CTRYAG 1 cut(s) 1462
BfrI CTTAAG 1 cut(s) 1297
BglI GCCNNNNNGGC 1 cut(s) 1361
BisI GCNGC 8 cut(s) 69, 741, 834, 887, 1303, 1338, 1353, 1520
BlpI GCTNAGC 1 cut(s) 1365
BlsI GCNGC 8 cut(s) 70, 742, 835, 888, 1304, 1339, 1354, 1521
Bme18I GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1602
BmeT110I CYCGRG 2 cut(s) 518, 905
BmgBI CACGTC 1 cut(s) 1273
BpiI GAAGAC 3 cut(s) 337, 1502, 1532
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 3 cut(s) 137, 445, 1268
Bpu1102I GCTNAGC 1 cut(s) 1365
BpuMI CCSGG 6 cut(s) 519, 520, 893, 949, 1166, 1235
Bsa29I ATCGAT 1 cut(s) 1261
BsaAI YACGTR 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 6 cut(s) 297, 587, 1218, 1391, 1422, 1529
BsaWI WCCGGW 1 cut(s) 1072
Bsc4I CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1079
Bse118I RCCGGY 2 cut(s) 538, 1211
Bse1I ACTGG 3 cut(s) 462, 662, 1605
Bse8I GATNNNNATC 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 1072
BseBI CCWGG 4 cut(s) 50, 127, 194, 970
BseCI ATCGAT 1 cut(s) 1261
BseGI GGATG 2 cut(s) 405, 1066
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1079
BseMII CTCAG 1 cut(s) 1550
BseNI ACTGG 3 cut(s) 462, 662, 1605
BseRI GAGGAG 2 cut(s) 308, 442
BseX3I CGGCCG 3 cut(s) 536, 945, 1353
BseXI GCAGC 4 cut(s) 80, 873, 1314, 1506
BseYI CCCAGC 1 cut(s) 856
Bsh1236I CGCG 4 cut(s) 836, 1058, 1340, 1400
Bsh1285I CGRYCG 4 cut(s) 75, 539, 948, 1356
BshFI GGCC 9 cut(s) 506, 538, 734, 740, 896, 947, 1355, 1429, 1435
BshNI GGYRCC 1 cut(s) 1489
BshVI ATCGAT 1 cut(s) 1261
BsiEI CGRYCG 4 cut(s) 75, 539, 948, 1356
BsiHKAI GWGCWC 3 cut(s) 318, 625, 868
BsiHKCI CYCGRG 2 cut(s) 518, 905
BsiWI CGTACG 1 cut(s) 489
BslFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BslI CCNNNNNNNGG 6 cut(s) 126, 259, 260, 747, 750, 1079
BsmAI GTCTC 4 cut(s) 155, 205, 313, 1382
BsmBI CGTCTC 2 cut(s) 205, 1382
BsmFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BsnI GGCC 9 cut(s) 506, 538, 734, 740, 896, 947, 1355, 1429, 1435
BsoBI CYCGRG 2 cut(s) 518, 905
Bsp1286I GDGCHC 3 cut(s) 318, 625, 868
Bsp13I TCCGGA 1 cut(s) 1072
Bsp143I GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
Bsp1720I GCTNAGC 1 cut(s) 1365
Bsp19I CCATGG 1 cut(s) 735
BspANI GGCC 9 cut(s) 506, 538, 734, 740, 896, 947, 1355, 1429, 1435
BspCNI CTCAG 1 cut(s) 1551
BspDI ATCGAT 1 cut(s) 1261
BspEI TCCGGA 1 cut(s) 1072
BspFNI CGCG 4 cut(s) 836, 1058, 1340, 1400
BspPI GGATC 6 cut(s) 509, 796, 1064, 1077, 1257, 1266
BspT107I GGYRCC 1 cut(s) 1489
BspTI CTTAAG 1 cut(s) 1297
BsrFI RCCGGY 2 cut(s) 538, 1211
BsrI ACTGG 3 cut(s) 462, 662, 1605
BssAI RCCGGY 2 cut(s) 538, 1211
BssMI GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
BssNI GRCGYC 6 cut(s) 297, 587, 1218, 1391, 1422, 1529
BssT1I CCWWGG 2 cut(s) 735, 1266
Bst2UI CCWGG 4 cut(s) 50, 127, 194, 970
Bst4CI ACNGT 6 cut(s) 143, 307, 469, 782, 1042, 1501
BstACI GRCGYC 6 cut(s) 297, 587, 1218, 1391, 1422, 1529
BstAFI CTTAAG 1 cut(s) 1297
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 4 cut(s) 159, 540, 544, 884
BstDEI CTNAG 3 cut(s) 58, 1365, 1559
BstDSI CCRYGG 6 cut(s) 204, 728, 735, 744, 1338, 1356
BstF5I GGATG 2 cut(s) 405, 1066
BstFNI CGCG 4 cut(s) 836, 1058, 1340, 1400
BstHHI GCGC 3 cut(s) 833, 838, 1400
BstKTI GATC 9 cut(s) 75, 439, 517, 799, 804, 1072, 1261, 1265, 1278
BstMAI GTCTC 4 cut(s) 155, 205, 313, 1382
BstMBI GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
BstMCI CGRYCG 4 cut(s) 75, 539, 948, 1356
BstMWI GCNNNNNNNGC 2 cut(s) 740, 1361
BstNI CCWGG 4 cut(s) 50, 127, 194, 970
BstNSI RCATGY 2 cut(s) 829, 856
BstSFI CTRYAG 1 cut(s) 1462
BstUI CGCG 4 cut(s) 836, 1058, 1340, 1400
BstV1I GCAGC 4 cut(s) 80, 873, 1314, 1506
BstV2I GAAGAC 3 cut(s) 337, 1502, 1532
BstX2I RGATCY 1 cut(s) 1069
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 1 cut(s) 1069
BstZI CGGCCG 3 cut(s) 536, 945, 1353
Bsu15I ATCGAT 1 cut(s) 1261
BsuRI GGCC 9 cut(s) 506, 538, 734, 740, 896, 947, 1355, 1429, 1435
BsuTUI ATCGAT 1 cut(s) 1261
BtgI CCRYGG 6 cut(s) 204, 728, 735, 744, 1338, 1356
BtrI CACGTC 1 cut(s) 1273
BtsCI GGATG 2 cut(s) 405, 1066
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 610
Cac8I GCNNGC 4 cut(s) 159, 540, 544, 884
CfoI GCGC 3 cut(s) 833, 838, 1400
Cfr10I RCCGGY 2 cut(s) 538, 1211
Cfr42I CCGCGG 1 cut(s) 1341
Cfr9I CCCGGG 1 cut(s) 518
ClaI ATCGAT 1 cut(s) 1261
CseI GACGC 4 cut(s) 305, 1064, 1129, 1399
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 4 cut(s) 232, 465, 490, 928
CviAII CATG 8 cut(s) 229, 256, 707, 736, 826, 853, 1144, 1486
CviQI GTAC 4 cut(s) 232, 465, 490, 928
DdeI CTNAG 3 cut(s) 58, 1365, 1559
DpnI GATC 9 cut(s) 74, 438, 516, 798, 803, 1071, 1260, 1264, 1277
DpnII GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
DraIII CACNNNGTG 1 cut(s) 817
EaeI YGGCCR 5 cut(s) 504, 536, 738, 945, 1353
EagI CGGCCG 3 cut(s) 536, 945, 1353
EciI GGCGGA 2 cut(s) 858, 876
Ecl136II GAGCTC 2 cut(s) 623, 866
EclXI CGGCCG 3 cut(s) 536, 945, 1353
Eco130I CCWWGG 2 cut(s) 735, 1266
Eco24I GRGCYC 2 cut(s) 625, 868
Eco47I GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1602
Eco52I CGGCCG 3 cut(s) 536, 945, 1353
Eco53kI GAGCTC 2 cut(s) 623, 866
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 2 cut(s) 518, 905
EcoICRI GAGCTC 2 cut(s) 623, 866
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 4 cut(s) 48, 125, 192, 968
EcoT14I CCWWGG 2 cut(s) 735, 1266
EcoT38I GRGCYC 2 cut(s) 625, 868
ErhI CCWWGG 2 cut(s) 735, 1266
Esp3I CGTCTC 2 cut(s) 205, 1382
FaeI CATG 8 cut(s) 232, 259, 710, 739, 829, 856, 1147, 1489
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
FatI CATG 8 cut(s) 228, 255, 706, 735, 825, 852, 1143, 1485
FauI CCCGC 4 cut(s) 232, 296, 1127, 1277
FbaI TGATCA 1 cut(s) 1275
FblI GTMKAC 4 cut(s) 78, 351, 1532, 1586
Fnu4HI GCNGC 8 cut(s) 69, 741, 834, 887, 1303, 1338, 1353, 1520
FokI GGATG 2 cut(s) 392, 1073
FriOI GRGCYC 2 cut(s) 625, 868
Fsp4HI GCNGC 8 cut(s) 69, 741, 834, 887, 1303, 1338, 1353, 1520
FspBI CTAG 1 cut(s) 18
GlaI GCGC 3 cut(s) 832, 837, 1399
GluI GCNGC 8 cut(s) 69, 741, 834, 887, 1303, 1338, 1353, 1520
GsaI CCCAGC 1 cut(s) 860
GsuI CTGGAG 3 cut(s) 137, 445, 1268
HaeIII GGCC 9 cut(s) 506, 538, 734, 740, 896, 947, 1355, 1429, 1435
HgaI GACGC 4 cut(s) 305, 1064, 1129, 1399
HhaI GCGC 3 cut(s) 833, 838, 1400
Hin1I GRCGYC 6 cut(s) 297, 587, 1218, 1391, 1422, 1529
Hin1II CATG 8 cut(s) 232, 259, 710, 739, 829, 856, 1147, 1489
Hin6I GCGC 3 cut(s) 831, 836, 1398
HinP1I GCGC 3 cut(s) 831, 836, 1398
HincII GTYRAC 4 cut(s) 352, 472, 936, 1533
HindII GTYRAC 4 cut(s) 352, 472, 936, 1533
HinfI GANTC 8 cut(s) 332, 637, 820, 899, 919, 1076, 1316, 1543
HphI GGTGA 1 cut(s) 1354
Hpy166II GTNNAC 9 cut(s) 79, 352, 472, 610, 690, 936, 1345, 1533, 1587
Hpy188I TCNGA 4 cut(s) 597, 801, 1108, 1548
Hpy188III TCNNGA 5 cut(s) 184, 261, 805, 1073, 1247
Hpy8I GTNNAC 9 cut(s) 79, 352, 472, 610, 690, 936, 1345, 1533, 1587
HpyAV CCTTC 5 cut(s) 776, 885, 1000, 1012, 1438
HpyCH4III ACNGT 6 cut(s) 143, 307, 469, 782, 1042, 1501
HpyCH4IV ACGT 7 cut(s) 488, 587, 633, 1218, 1272, 1422, 1529
HpyCH4V TGCA 3 cut(s) 26, 710, 1231
HpyF10VI GCNNNNNNNGC 2 cut(s) 740, 1361
HpyF3I CTNAG 3 cut(s) 58, 1365, 1559
HpySE526I ACGT 7 cut(s) 488, 587, 633, 1218, 1272, 1422, 1529
Hsp92I GRCGYC 6 cut(s) 297, 587, 1218, 1391, 1422, 1529
Hsp92II CATG 8 cut(s) 232, 259, 710, 739, 829, 856, 1147, 1489
HspAI GCGC 3 cut(s) 831, 836, 1398
KflI GGGWCCC 1 cut(s) 553
Kpn2I TCCGGA 1 cut(s) 1072
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Ksp22I TGATCA 1 cut(s) 1275
KspI CCGCGG 1 cut(s) 1341
Kzo9I GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
LmnI GCTCC 6 cut(s) 263, 321, 598, 863, 871, 894
Lsp1109I GCAGC 4 cut(s) 80, 873, 1314, 1506
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 7 cut(s) 488, 587, 633, 1218, 1272, 1422, 1529
MaeIII GTNAC 2 cut(s) 817, 920
MalI GATC 9 cut(s) 74, 438, 516, 798, 803, 1071, 1260, 1264, 1277
MboI GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
MboII GAAGA 4 cut(s) 337, 1507, 1537, 1552
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 3 cut(s) 318, 625, 868
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 1 cut(s) 1480
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 4 cut(s) 646, 814, 928, 1325
MmeI TCCRAC 3 cut(s) 346, 1024, 1086
Mox20I TGGCCA 1 cut(s) 506
MroI TCCGGA 1 cut(s) 1072
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 506
MseI TTAA 1 cut(s) 1298
MslI CAYNNNNRTG 1 cut(s) 711
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 3 cut(s) 10, 157, 1340
MspCI CTTAAG 1 cut(s) 1297
MteI GCGCNGCGC 1 cut(s) 834
MvaI CCWGG 4 cut(s) 50, 127, 194, 970
MvnI CGCG 4 cut(s) 836, 1058, 1340, 1400
MwoI GCNNNNNNNGC 2 cut(s) 740, 1361
NaeI GCCGGC 1 cut(s) 540
NciI CCSGG 6 cut(s) 519, 520, 893, 949, 1166, 1235
NcoI CCATGG 1 cut(s) 735
NdeII GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 8 cut(s) 232, 259, 710, 739, 829, 856, 1147, 1489
NmeAIII GCCGAG 1 cut(s) 1455
NmuCI GTSAC 2 cut(s) 817, 920
NspI RCATGY 2 cut(s) 829, 856
PciI ACATGT 1 cut(s) 825
PcsI WCGNNNNNNNCGW 6 cut(s) 81, 348, 593, 1007, 1353, 1397
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 4 cut(s) 332, 899, 1076, 1543
Pfl23II CGTACG 1 cut(s) 489
PflFI GACNNNGTC 2 cut(s) 586, 1600
PkrI GCNGC 8 cut(s) 70, 742, 835, 888, 1304, 1339, 1354, 1521
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 4 cut(s) 645, 814, 927, 1324
PpsI GAGTC 4 cut(s) 645, 814, 927, 1324
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 553
PscI ACATGT 1 cut(s) 825
Psp124BI GAGCTC 2 cut(s) 625, 868
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 4 cut(s) 48, 125, 192, 968
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 4 cut(s) 48, 125, 192, 968
PspLI CGTACG 1 cut(s) 489
PspPPI RGGWCCY 1 cut(s) 553
PsuI RGATCY 1 cut(s) 1069
PsyI GACNNNGTC 2 cut(s) 586, 1600
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 4 cut(s) 233, 466, 491, 929
RsaNI GTAC 4 cut(s) 232, 465, 490, 928
RseI CAYNNNNRTG 1 cut(s) 711
SacI GAGCTC 2 cut(s) 625, 868
SacII CCGCGG 1 cut(s) 1341
SalI GTCGAC 2 cut(s) 350, 1531
SaqAI TTAA 1 cut(s) 1298
SatI GCNGC 8 cut(s) 69, 741, 834, 887, 1303, 1338, 1353, 1520
Sau3AI GATC 9 cut(s) 72, 436, 514, 796, 801, 1069, 1258, 1262, 1275
SchI GAGTC 4 cut(s) 646, 814, 928, 1325
SduI GDGCHC 3 cut(s) 318, 625, 868
SexAI ACCWGGT 1 cut(s) 125
SfcI CTRYAG 1 cut(s) 1462
Sfr303I CCGCGG 1 cut(s) 1341
SgrAI CRCCGGYG 1 cut(s) 1211
SgrBI CCGCGG 1 cut(s) 1341
SgrDI CGTCGACG 1 cut(s) 1531
SinI GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1602
SmaI CCCGGG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 1 cut(s) 1297
SmoI CTYRAG 1 cut(s) 1297
Sse9I AATT 1 cut(s) 1480
SspI AATATT 1 cut(s) 1579
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 2 cut(s) 625, 868
StyI CCWWGG 2 cut(s) 735, 1266
TaaI ACNGT 6 cut(s) 143, 307, 469, 782, 1042, 1501
TaiI ACGT 7 cut(s) 491, 590, 636, 1221, 1275, 1425, 1532
TaqI TCGA 8 cut(s) 145, 294, 351, 654, 777, 878, 1261, 1532
TaqII GACCGA 2 cut(s) 78, 1067
TasI AATT 1 cut(s) 1480
TauI GCSGC 4 cut(s) 743, 836, 1340, 1355
TfiI GAWTC 4 cut(s) 332, 899, 1076, 1543
Tru1I TTAA 1 cut(s) 1298
Tru9I TTAA 1 cut(s) 1298
TscAI CASTG 1 cut(s) 617
TseFI GTSAC 2 cut(s) 817, 920
TseI GCWGC 4 cut(s) 68, 886, 1302, 1519
Tsp45I GTSAC 2 cut(s) 817, 920
TspDTI ATGAA 2 cut(s) 466, 1474
TspGWI ACGGA 4 cut(s) 193, 301, 1016, 1454
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 1 cut(s) 617
Tth111I GACNNNGTC 2 cut(s) 586, 1600
Vha464I CTTAAG 1 cut(s) 1297
VpaK11BI GGWCC 9 cut(s) 4, 129, 532, 553, 607, 690, 753, 782, 1602
XapI RAATTY 1 cut(s) 1480
XceI RCATGY 2 cut(s) 829, 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 4 cut(s) 78, 351, 1532, 1586
XspI CTAG 1 cut(s) 18
ZraI GACGTC 4 cut(s) 588, 1219, 1423, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.