RchiOBHm_Chr4g0432931

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000677
Physical Location & Seq
Forward (+)
57029953 .. 57030354
402 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40150

Sequence Viewer

Length: 402 bp
ATGGTGGAGAGTGGCGACCCGGACTTCCAGTGGGCCCTGCCGGAGAACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACGACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCCCATGCTTCCATTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGCTGTTTATTGAGGAGCTGCCGAAGACGTCGAAATTTGTATGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

133

Amino Acids

15.23

Weight (kDa)

6.74

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 14 - 49 4.1e-09 AMP-binding enzyme
AMP-binding_C PF13193 85 - 128 1.1e-07 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 340
AccB7I CCANNNNNTGG 1 cut(s) 220
AciI CCGC 1 cut(s) 280
AclWI GGATC 2 cut(s) 233, 246
AcsI RAATTY 2 cut(s) 331, 386
AcyI GRCGYC 1 cut(s) 380
AfaI GTAC 3 cut(s) 169, 234, 311
AfiI CCNNNNNNNGG 2 cut(s) 161, 220
AhdI GACNNNNNGTC 1 cut(s) 180
AjiI CACGTC 1 cut(s) 274
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AluBI AGCT 3 cut(s) 226, 325, 370
AluI AGCT 3 cut(s) 226, 325, 370
AlwI GGATC 2 cut(s) 233, 246
AoxI GGCC 3 cut(s) 33, 163, 284
ApaI GGGCCC 1 cut(s) 37
ApeKI GCWGC 2 cut(s) 325, 370
ApoI RAATTY 2 cut(s) 331, 386
AspS9I GGNCC 5 cut(s) 33, 34, 55, 109, 163
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 130
AvaII GGWCC 2 cut(s) 55, 109
BaeGI GKGCMC 1 cut(s) 37
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 1 cut(s) 340
BanII GRGCYC 2 cut(s) 37, 129
BbsI GAAGAC 2 cut(s) 353, 383
BbvI GCAGC 2 cut(s) 312, 357
BceAI ACGGC 2 cut(s) 150, 233
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BcnI CCSGG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 313
BisI GCNGC 2 cut(s) 326, 371
BlsI GCNGC 2 cut(s) 327, 372
BmcAI AGTACT 1 cut(s) 311
Bme1390I CCNGG 1 cut(s) 20
Bme18I GGWCC 2 cut(s) 55, 109
BmeRI GACNNNNNGTC 1 cut(s) 180
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 5 cut(s) 33, 34, 55, 109, 163
BmiI GGNNCC 5 cut(s) 35, 56, 57, 240, 342
BmrFI CCNGG 1 cut(s) 20
BmrI ACTGGG 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 170
BpiI GAAGAC 2 cut(s) 353, 383
BpmI CTGGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 20
BsaHI GRCGYC 1 cut(s) 380
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 220
Bse1I ACTGG 2 cut(s) 28, 165
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 2 cut(s) 161, 220
BseNI ACTGG 2 cut(s) 28, 165
BseRI GAGGAG 2 cut(s) 144, 380
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 312, 357
BshFI GGCC 3 cut(s) 35, 165, 286
BshNI GGYRCC 1 cut(s) 340
BsiSI CCGG 3 cut(s) 20, 41, 81
BsiWI CGTACG 1 cut(s) 232
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 161, 220
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 3 cut(s) 35, 165, 286
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 37, 129
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 1 cut(s) 280
BspANI GGCC 3 cut(s) 35, 165, 286
BspLI GGNNCC 5 cut(s) 35, 56, 57, 240, 342
BspMAI CTGCAG 1 cut(s) 317
BspPI GGATC 2 cut(s) 233, 246
BspQI GCTCTTC 1 cut(s) 134
BspT107I GGYRCC 1 cut(s) 340
BsrI ACTGG 2 cut(s) 28, 165
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 1 cut(s) 238
BssNI GRCGYC 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 2 cut(s) 142, 352
Bst6I CTCTTC 1 cut(s) 134
BstACI GRCGYC 1 cut(s) 380
BstKTI GATC 1 cut(s) 241
BstMBI GATC 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 18
BstSFI CTRYAG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 312, 357
BstV2I GAAGAC 2 cut(s) 353, 383
BstX2I RGATCY 1 cut(s) 238
BstXI CCANNNNNNTGG 1 cut(s) 106
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 3 cut(s) 35, 165, 286
BtrI CACGTC 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 2 cut(s) 35, 112
Cfr13I GGNCC 5 cut(s) 33, 34, 55, 109, 163
CseI GACGC 2 cut(s) 73, 203
Csp6I GTAC 3 cut(s) 168, 233, 310
CviAII CATG 2 cut(s) 244, 337
CviQI GTAC 3 cut(s) 168, 233, 310
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
DriI GACNNNNNGTC 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 134
Eam1105I GACNNNNNGTC 1 cut(s) 180
EarI CTCTTC 1 cut(s) 134
EciI GGCGGA 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 37, 129
Eco47I GGWCC 2 cut(s) 55, 109
EcoO109I RGGNCCY 2 cut(s) 34, 55
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 37, 129
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 2 cut(s) 247, 340
FaiI YATR 3 cut(s) 245, 338, 394
FaqI GGGAC 1 cut(s) 68
FatI CATG 2 cut(s) 243, 336
Fnu4HI GCNGC 2 cut(s) 326, 371
FriOI GRGCYC 2 cut(s) 37, 129
Fsp4HI GCNGC 2 cut(s) 326, 371
GluI GCNGC 2 cut(s) 326, 371
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 3 cut(s) 35, 165, 286
HapII CCGG 3 cut(s) 20, 41, 81
HgaI GACGC 2 cut(s) 73, 203
Hin1I GRCGYC 1 cut(s) 380
Hin1II CATG 2 cut(s) 247, 340
HpaII CCGG 3 cut(s) 20, 41, 81
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 2 cut(s) 292, 385
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 142, 352
HpyCH4IV ACGT 2 cut(s) 273, 380
HpyCH4V TGCA 1 cut(s) 315
HpySE526I ACGT 2 cut(s) 273, 380
Hsp92I GRCGYC 1 cut(s) 380
Hsp92II CATG 2 cut(s) 247, 340
KflI GGGWCCC 1 cut(s) 55
Kzo9I GATC 1 cut(s) 238
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 2 cut(s) 223, 367
Lsp1109I GCAGC 2 cut(s) 312, 357
MaeII ACGT 2 cut(s) 273, 380
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 3 cut(s) 121, 358, 388
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 37, 129
MluCI AATT 2 cut(s) 331, 386
MnlI CCTC 6 cut(s) 103, 116, 165, 276, 311, 358
MspI CCGG 3 cut(s) 20, 41, 81
MspR9I CCNGG 1 cut(s) 20
NciI CCSGG 1 cut(s) 20
NdeII GATC 1 cut(s) 238
NlaIII CATG 2 cut(s) 247, 340
NlaIV GGNNCC 5 cut(s) 35, 56, 57, 240, 342
PciSI GCTCTTC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 163
Pfl23II CGTACG 1 cut(s) 232
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 2 cut(s) 327, 372
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
PspLI CGTACG 1 cut(s) 232
PspN4I GGNNCC 5 cut(s) 35, 56, 57, 240, 342
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 5 cut(s) 33, 34, 55, 109, 163
PspPPI RGGWCCY 1 cut(s) 55
PstI CTGCAG 1 cut(s) 317
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 3 cut(s) 169, 234, 311
RsaNI GTAC 3 cut(s) 168, 233, 310
SapI GCTCTTC 1 cut(s) 134
SatI GCNGC 2 cut(s) 326, 371
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 5 cut(s) 33, 34, 55, 109, 163
ScaI AGTACT 1 cut(s) 311
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 2 cut(s) 37, 129
SetI ASST 5 cut(s) 228, 276, 327, 372, 383
SfcI CTRYAG 1 cut(s) 313
SinI GGWCC 2 cut(s) 55, 109
Sse9I AATT 2 cut(s) 331, 386
SsiI CCGC 1 cut(s) 280
StyD4I CCNGG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 142, 352
TaiI ACGT 2 cut(s) 276, 383
TaqI TCGA 2 cut(s) 157, 383
TasI AATT 2 cut(s) 331, 386
TatI WGTACW 1 cut(s) 309
TscAI CASTG 2 cut(s) 35, 119
TseI GCWGC 2 cut(s) 325, 370
TspDTI ATGAA 2 cut(s) 121, 325
TspGWI ACGGA 1 cut(s) 305
TspRI CASTG 2 cut(s) 35, 119
Van91I CCANNNNNTGG 1 cut(s) 220
VpaK11BI GGWCC 2 cut(s) 55, 109
XapI RAATTY 2 cut(s) 331, 386
ZraI GACGTC 1 cut(s) 381
ZrmI AGTACT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.