Rh3CG316500

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000677
Physical Location & Seq
Reverse (-)
33790823 .. 33791296
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG316500.1

Sequence Viewer

Length: 474 bp
ATGTTGGGTTACCTCAAAGACCCGGAAACAACGAGCAAAACCCTGAAACAGAACGGGTGGTTTTACACCGGCGACGTCGGGGTTATGCACCCGGACGGGTATCTAGAGATAAAGGATCGATCTAACGACGTGATCATAAGCGGGGGAGAGAACTTAAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCCGCCGTGAACGAGGCGGCCGTGGTGGCTAAGGCTGACGAGTTCTGGGGGGAGACACCGTGCGCATTTGTCAGCCTGAAAGCTGATGTCAAGCAGAGGCCGACAGAGAAGGAGATAATAGAGTTTTGTAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTCAAGGACGAGCTGCCGAAGACGTCGACGGGGAAGATTCAGAAGCCTATGCTGAGAGAGATTGCGAAAAATATTGGGTCTACGTCAACAGAGAGGTCCAGTCGGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

157

Amino Acids

17.41

Weight (kDa)

5.93

Isoelectric Point (pI)

31.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 56 - 133 2.7e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 78, 389
Acc16I TGCGCA 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 346
AccI GTMKAC 2 cut(s) 389, 443
AciI CCGC 3 cut(s) 141, 195, 209
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 337
AcyI GRCGYC 2 cut(s) 75, 386
AflII CTTAAG 1 cut(s) 154
AgsI TTSAA 1 cut(s) 367
AjiI CACGTC 1 cut(s) 130
AluBI AGCT 3 cut(s) 275, 331, 376
AluI AGCT 3 cut(s) 275, 331, 376
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 123
AoxI GGCC 2 cut(s) 210, 290
ApeKI GCWGC 3 cut(s) 159, 331, 376
ApoI RAATTY 1 cut(s) 337
AspLEI GCGC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 459
AsuC2I CCSGG 2 cut(s) 23, 92
AvaII GGWCC 1 cut(s) 459
BanI GGYRCC 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 359, 389
BbvI GCAGC 3 cut(s) 171, 318, 363
BceAI ACGGC 2 cut(s) 182, 197
BclI TGATCA 1 cut(s) 132
BcnI CCSGG 2 cut(s) 23, 92
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 1 cut(s) 104
BfrI CTTAAG 1 cut(s) 154
BglI GCCNNNNNGGC 1 cut(s) 218
BisI GCNGC 5 cut(s) 160, 195, 210, 332, 377
BlsI GCNGC 5 cut(s) 161, 196, 211, 333, 378
Bme1390I CCNGG 2 cut(s) 23, 92
Bme18I GGWCC 1 cut(s) 459
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 459
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 2 cut(s) 23, 92
BpiI GAAGAC 2 cut(s) 359, 389
Bpu10I CCTNAGC 1 cut(s) 222
BpuMI CCSGG 2 cut(s) 23, 92
Bsa29I ATCGAT 1 cut(s) 118
BsaHI GRCGYC 2 cut(s) 75, 386
BsaJI CCNNGG 1 cut(s) 213
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 236, 266
Bse118I RCCGGY 1 cut(s) 68
Bse1I ACTGG 1 cut(s) 462
BseCI ATCGAT 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 462
BseX3I CGGCCG 1 cut(s) 210
BseXI GCAGC 3 cut(s) 171, 318, 363
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 212, 292
BshNI GGYRCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 118
BsiEI CGRYCG 1 cut(s) 213
BsiSI CCGG 3 cut(s) 23, 69, 92
BsmAI GTCTC 1 cut(s) 239
BsnI GGCC 2 cut(s) 212, 292
Bsp143I GATC 3 cut(s) 115, 119, 132
BspACI CCGC 3 cut(s) 141, 195, 209
BspANI GGCC 2 cut(s) 212, 292
BspCNI CTCAG 1 cut(s) 408
BspDI ATCGAT 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 346
BspTI CTTAAG 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 68
BsrI ACTGG 1 cut(s) 462
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 3 cut(s) 115, 119, 132
BssNI GRCGYC 2 cut(s) 75, 386
Bst4CI ACNGT 2 cut(s) 252, 358
BstACI GRCGYC 2 cut(s) 75, 386
BstAFI CTTAAG 1 cut(s) 154
BstDEI CTNAG 2 cut(s) 222, 416
BstDSI CCRYGG 1 cut(s) 213
BstEII GGTNACC 1 cut(s) 8
BstHHI GCGC 1 cut(s) 257
BstKTI GATC 3 cut(s) 118, 122, 135
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 3 cut(s) 115, 119, 132
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 1 cut(s) 218
BstPI GGTNACC 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 21, 90
BstV1I GCAGC 3 cut(s) 171, 318, 363
BstV2I GAAGAC 2 cut(s) 359, 389
BstZI CGGCCG 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 118
BsuRI GGCC 2 cut(s) 212, 292
BsuTUI ATCGAT 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 213
BtrI CACGTC 1 cut(s) 130
CfoI GCGC 1 cut(s) 257
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 459
ClaI ATCGAT 1 cut(s) 118
CviAII CATG 1 cut(s) 343
CviJI RGCY 9 cut(s) 212, 221, 227, 267, 275, 292, 331, 376, 409
CviKI_1 RGCY 9 cut(s) 212, 221, 227, 267, 275, 292, 331, 376, 409
DdeI CTNAG 2 cut(s) 222, 416
DpnI GATC 3 cut(s) 117, 121, 134
DpnII GATC 3 cut(s) 115, 119, 132
EaeI YGGCCR 1 cut(s) 210
EagI CGGCCG 1 cut(s) 210
EclXI CGGCCG 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 459
Eco52I CGGCCG 1 cut(s) 210
Eco91I GGTNACC 1 cut(s) 8
EcoO65I GGTNACC 1 cut(s) 8
FaeI CATG 1 cut(s) 346
FaiI YATR 4 cut(s) 86, 137, 344, 413
FatI CATG 1 cut(s) 342
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 132
FblI GTMKAC 2 cut(s) 389, 443
Fnu4HI GCNGC 5 cut(s) 160, 195, 210, 332, 377
Fsp4HI GCNGC 5 cut(s) 160, 195, 210, 332, 377
FspAI RTGCGCAY 1 cut(s) 256
FspBI CTAG 1 cut(s) 104
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 1 cut(s) 256
GluI GCNGC 5 cut(s) 160, 195, 210, 332, 377
HaeIII GGCC 2 cut(s) 212, 292
HapII CCGG 3 cut(s) 23, 69, 92
HhaI GCGC 1 cut(s) 257
Hin1I GRCGYC 2 cut(s) 75, 386
Hin1II CATG 1 cut(s) 346
Hin6I GCGC 1 cut(s) 255
HinP1I GCGC 1 cut(s) 255
HincII GTYRAC 2 cut(s) 390, 450
HindII GTYRAC 2 cut(s) 390, 450
HinfI GANTC 2 cut(s) 173, 400
HpaII CCGG 3 cut(s) 23, 69, 92
Hpy166II GTNNAC 4 cut(s) 202, 390, 444, 450
Hpy188I TCNGA 1 cut(s) 405
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 4 cut(s) 202, 390, 444, 450
Hpy99I CGWCG 5 cut(s) 77, 80, 131, 391, 394
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 2 cut(s) 252, 358
HpyCH4IV ACGT 4 cut(s) 75, 129, 386, 446
HpyCH4V TGCA 1 cut(s) 88
HpyF10VI GCNNNNNNNGC 1 cut(s) 218
HpyF3I CTNAG 2 cut(s) 222, 416
HpySE526I ACGT 4 cut(s) 75, 129, 386, 446
Hsp92I GRCGYC 2 cut(s) 75, 386
Hsp92II CATG 1 cut(s) 346
HspAI GCGC 1 cut(s) 255
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 3 cut(s) 115, 119, 132
LpnPI CCDG 6 cut(s) 36, 56, 82, 105, 223, 281
Lsp1109I GCAGC 3 cut(s) 171, 318, 363
MaeI CTAG 1 cut(s) 104
MaeII ACGT 4 cut(s) 75, 129, 386, 446
MaeIII GTNAC 1 cut(s) 8
MalI GATC 3 cut(s) 117, 121, 134
MboI GATC 3 cut(s) 115, 119, 132
MboII GAAGA 3 cut(s) 364, 394, 409
MluCI AATT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 6 cut(s) 23, 160, 199, 282, 317, 450
MseI TTAA 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 154
MspI CCGG 3 cut(s) 23, 69, 92
MspR9I CCNGG 2 cut(s) 23, 92
MwoI GCNNNNNNNGC 1 cut(s) 218
NciI CCSGG 2 cut(s) 23, 92
NdeII GATC 3 cut(s) 115, 119, 132
NlaIII CATG 1 cut(s) 346
NlaIV GGNNCC 1 cut(s) 348
NsbI TGCGCA 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 5 cut(s) 161, 196, 211, 333, 378
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
PspEI GGTNACC 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 348
PspPI GGNCC 1 cut(s) 459
SalI GTCGAC 1 cut(s) 388
SaqAI TTAA 1 cut(s) 155
SatI GCNGC 5 cut(s) 160, 195, 210, 332, 377
Sau3AI GATC 3 cut(s) 115, 119, 132
Sau96I GGNCC 1 cut(s) 459
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 23, 92
SgrAI CRCCGGYG 1 cut(s) 68
SgrDI CGTCGACG 1 cut(s) 388
SinI GGWCC 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 1 cut(s) 337
SsiI CCGC 3 cut(s) 141, 195, 209
SspI AATATT 1 cut(s) 436
SspMI CTAG 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 21, 90
TaaI ACNGT 2 cut(s) 252, 358
TaiI ACGT 4 cut(s) 78, 132, 389, 449
TaqI TCGA 3 cut(s) 118, 171, 389
TasI AATT 1 cut(s) 337
TauI GCSGC 2 cut(s) 197, 212
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 155
Tru9I TTAA 1 cut(s) 155
TseI GCWGC 3 cut(s) 159, 331, 376
TspDTI ATGAA 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 154
VpaK11BI GGWCC 1 cut(s) 459
XapI RAATTY 1 cut(s) 337
XbaI TCTAGA 1 cut(s) 103
XmiI GTMKAC 2 cut(s) 389, 443
XspI CTAG 1 cut(s) 104
ZraI GACGTC 2 cut(s) 76, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.