Rh3CG316700

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000677
Physical Location & Seq
Reverse (-)
33838931 .. 33845684
6754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG316700.1

Sequence Viewer

Length: 1860 bp
ATGGAGGAGCTGAAGCCAACCCCCGCCAACTCATCCCCGCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGAACACACCACCTACACGTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCCTCCTCCATCTCCTCCCTCGGCGTCCGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCCGCACCGTCTCCCTCCTCCTCCGCCACAGCCAGTCCAAGCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGACGCCCTCCATCTCTTCCCTCCCAACACCCCCACCCCCCTCCTCGTCCTCATCGCCGACGACGATGCACCTGCACCCCATCCCAACTCCTCCGACTCATCCCCCTTCTGCGCCACGTACGAGGAAATGGTGGACAGCGGCGATCCGGACTTCCAGTGGGCCCTGCCGGAGAACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACTACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTGGGAGGGACCAACGTCTGCGTCCGCAAATTCGACGCGCCTGTCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACGAATGTACCGAACAACGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCGCCCGCAGCGGTTCTGGCTCGGACTGAGTCGCTGGGATTCGTGGTGAGCCACGGGTACGGAGGCGGGTGTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCGAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATCAAGGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTTCTCTACACCAACTCGGCGGTGAACGAGGCGGCCGTGGTGGCGAGGCCTGACGAGTTCTGGGGGGAGACGCCTTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTACTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTTATTGAGGAGCTGCCGAAGACGTCGACGGGGAAAATTCAGAAGTTCGTGCTGAGAGAGATGGCCAAGGCCTTGGGGTCCACAAGGCTGAGTCGGATAAACCTGGGCTTGACTGTCTCTGCCCTACATCCTCCAGACCCTCGTATGGAGTTCTCAAATCTCATCATGGAATCGACAGAGGAGCACAACCTGGTGTTGAAATTCATAGGCTGGACTGGATCCAGAGGTGACCCAAGTCCGAGTCAAGTTTCTCGACGACTAGAACTGACGCGGAAGCCGGAGTCGGTGGCGGTGGAGAAGCAAGTATGGTCCTGGCTGAAAAACCAGTCGTGGTGTTATGCTGATGATGGGGATGGTGTTGAGATTAGGAACAATGATGGCTCCTGTGCTAATACGATTGGAAATGGACCTAGAAGATCATTCAAACGCGATCTCGATAGCAGCCCAAAGAATCACCGGAGCCCATGGCAGCAGATTATTCAAACATATGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

619

Amino Acids

68.38

Weight (kDa)

6.15

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 330 2.8e-60 AMP-binding enzyme
AMP-binding_C PF13193 389 - 466 3.6e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 457
AasI GACNNNNNNGTC 1 cut(s) 791
AatII GACGTC 1 cut(s) 1388
Acc36I ACCTGC 1 cut(s) 457
AccB1I GGYRCC 2 cut(s) 263, 1345
AccB7I CCANNNNNTGG 1 cut(s) 723
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 3 cut(s) 78, 351, 1388
AccII CGCG 6 cut(s) 153, 189, 788, 1256, 1633, 1791
AccIII TCCGGA 1 cut(s) 523
AclWI GGATC 5 cut(s) 515, 749, 1113, 1575, 1588
AcoI YGGCCR 3 cut(s) 190, 1209, 1425
AcsI RAATTY 4 cut(s) 779, 1336, 1398, 1562
AcuI CTGAAG 1 cut(s) 32
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 6 cut(s) 180, 264, 381, 822, 1247, 1385
AfaI GTAC 7 cut(s) 233, 497, 672, 737, 873, 986, 1316
AfiI CCNNNNNNNGG 6 cut(s) 123, 260, 664, 723, 756, 1507
AflIII ACRYGT 2 cut(s) 120, 831
AgsI TTSAA 3 cut(s) 1561, 1786, 1844
AhdI GACNNNNNGTC 3 cut(s) 138, 683, 821
AjiI CACGTC 4 cut(s) 196, 1075, 1129, 1279
AjnI CCWGG 3 cut(s) 1464, 1551, 1673
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 566, 598
AluBI AGCT 8 cut(s) 10, 238, 340, 366, 729, 923, 1330, 1375
AluI AGCT 8 cut(s) 10, 238, 340, 366, 729, 923, 1330, 1375
Alw21I GWGCWC 3 cut(s) 240, 925, 1548
Alw26I GTCTC 4 cut(s) 205, 313, 1238, 1483
AlwI GGATC 5 cut(s) 515, 749, 1113, 1575, 1588
Aor13HI TCCGGA 1 cut(s) 523
AoxI GGCC 8 cut(s) 190, 537, 666, 1209, 1223, 1289, 1425, 1431
ApaI GGGCCC 1 cut(s) 541
ApeKI GCWGC 6 cut(s) 935, 1158, 1330, 1375, 1803, 1831
ApoI RAATTY 4 cut(s) 779, 1336, 1398, 1562
AspLEI GCGC 5 cut(s) 266, 491, 790, 844, 1256
AsuC2I CCSGG 2 cut(s) 899, 1091
AsuHPI GGTGA 7 cut(s) 34, 634, 985, 1093, 1210, 1601, 1808
AvaII GGWCC 8 cut(s) 559, 613, 759, 850, 901, 1440, 1671, 1769
BaeGI GKGCMC 1 cut(s) 541
BaeI ACNNNNGTAYC 2 cut(s) 855, 888
BalI TGGCCA 1 cut(s) 1427
BamHI GGATCC 1 cut(s) 1580
BanI GGYRCC 2 cut(s) 263, 1345
BanII GRGCYC 5 cut(s) 240, 541, 633, 925, 1826
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 1358, 1388
Bbv12I GWGCWC 3 cut(s) 240, 925, 1548
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 6 cut(s) 947, 1170, 1317, 1362, 1815, 1843
BccI CCATC 8 cut(s) 173, 287, 396, 465, 1417, 1703, 1709, 1733
BceAI ACGGC 9 cut(s) 97, 233, 251, 653, 734, 736, 835, 1050, 1196
BcgI CGANNNNNNTGC 4 cut(s) 425, 431, 459, 465
BciT130I CCWGG 3 cut(s) 1466, 1553, 1675
BclI TGATCA 2 cut(s) 807, 1131
BcnI CCSGG 2 cut(s) 899, 1091
BcoDI GTCTC 4 cut(s) 205, 313, 1238, 1483
BfaI CTAG 3 cut(s) 59, 1622, 1773
BfmI CTRYAG 1 cut(s) 1318
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 1 cut(s) 457
BglI GCCNNNNNGGC 1 cut(s) 1217
BmcAI AGTACT 1 cut(s) 1316
Bme1390I CCNGG 5 cut(s) 899, 1091, 1466, 1553, 1675
Bme18I GGWCC 8 cut(s) 559, 613, 759, 850, 901, 1440, 1671, 1769
BmeRI GACNNNNNGTC 3 cut(s) 138, 683, 821
BmgBI CACGTC 4 cut(s) 196, 1075, 1129, 1279
BmrFI CCNGG 5 cut(s) 899, 1091, 1466, 1553, 1675
BmrI ACTGGG 1 cut(s) 673
BmsI GCATC 3 cut(s) 134, 433, 1074
BmuI ACTGGG 1 cut(s) 673
BoxI GACNNNNGTC 2 cut(s) 764, 1596
BpiI GAAGAC 2 cut(s) 1358, 1388
BpmI CTGGAG 3 cut(s) 745, 1124, 1479
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 1174
BpuMI CCSGG 2 cut(s) 899, 1091
BsaAI YACGTR 2 cut(s) 123, 495
BsaHI GRCGYC 6 cut(s) 180, 264, 381, 822, 1247, 1385
BsaWI WCCGGW 2 cut(s) 523, 1818
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 539, 569, 1157, 1187
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 260, 664, 723, 756, 1507
Bse118I RCCGGY 2 cut(s) 844, 1067
Bse1I ACTGG 5 cut(s) 331, 532, 668, 1582, 1687
BseAI TCCGGA 1 cut(s) 523
BseBI CCWGG 3 cut(s) 1466, 1553, 1675
BseGI GGATG 6 cut(s) 32, 219, 457, 476, 1489, 1720
BseLI CCNNNNNNNGG 6 cut(s) 123, 260, 664, 723, 756, 1507
BseMII CTCAG 4 cut(s) 376, 945, 1406, 1442
BseNI ACTGG 5 cut(s) 331, 532, 668, 1582, 1687
BseSI GKGCMC 1 cut(s) 541
BseX3I CGGCCG 1 cut(s) 1209
BseXI GCAGC 6 cut(s) 947, 1170, 1317, 1362, 1815, 1843
BseYI CCCAGC 1 cut(s) 961
BsgI GTGCAG 1 cut(s) 435
Bsh1236I CGCG 6 cut(s) 153, 189, 788, 1256, 1633, 1791
Bsh1285I CGRYCG 1 cut(s) 1212
BshFI GGCC 8 cut(s) 192, 539, 668, 1211, 1225, 1291, 1427, 1433
BshNI GGYRCC 2 cut(s) 263, 1345
BsiEI CGRYCG 1 cut(s) 1212
BsiHKAI GWGCWC 3 cut(s) 240, 925, 1548
BsiWI CGTACG 2 cut(s) 495, 735
BslFI GGGAC 3 cut(s) 236, 572, 772
BslI CCNNNNNNNGG 6 cut(s) 123, 260, 664, 723, 756, 1507
BsmAI GTCTC 4 cut(s) 205, 313, 1238, 1483
BsmBI CGTCTC 3 cut(s) 205, 313, 1238
BsmFI GGGAC 3 cut(s) 236, 572, 772
BsnI GGCC 8 cut(s) 192, 539, 668, 1211, 1225, 1291, 1427, 1433
Bsp120I GGGCCC 1 cut(s) 537
Bsp1286I GDGCHC 6 cut(s) 240, 541, 633, 925, 1548, 1826
Bsp13I TCCGGA 1 cut(s) 523
Bsp19I CCATGG 1 cut(s) 1826
BspANI GGCC 8 cut(s) 192, 539, 668, 1211, 1225, 1291, 1427, 1433
BspCNI CTCAG 4 cut(s) 375, 946, 1407, 1443
BspEI TCCGGA 1 cut(s) 523
BspFNI CGCG 6 cut(s) 153, 189, 788, 1256, 1633, 1791
BspHI TCATGA 1 cut(s) 909
BspMAI CTGCAG 1 cut(s) 1322
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 5 cut(s) 515, 749, 1113, 1575, 1588
BspQI GCTCTTC 1 cut(s) 638
BspT107I GGYRCC 2 cut(s) 263, 1345
BsrBI CCGCTC 1 cut(s) 40
BsrFI RCCGGY 2 cut(s) 844, 1067
BsrI ACTGG 5 cut(s) 331, 532, 668, 1582, 1687
BssAI RCCGGY 2 cut(s) 844, 1067
BssNI GRCGYC 6 cut(s) 180, 264, 381, 822, 1247, 1385
BssT1I CCWWGG 5 cut(s) 603, 1122, 1428, 1434, 1826
Bst2UI CCWGG 3 cut(s) 1466, 1553, 1675
Bst4CI ACNGT 7 cut(s) 76, 140, 307, 646, 1055, 1357, 1477
Bst6I CTCTTC 3 cut(s) 350, 398, 638
BstACI GRCGYC 6 cut(s) 180, 264, 381, 822, 1247, 1385
BstBAI YACGTR 2 cut(s) 123, 495
BstC8I GCNNGC 1 cut(s) 933
BstDEI CTNAG 4 cut(s) 362, 954, 1415, 1451
BstDSI CCRYGG 4 cut(s) 750, 979, 1212, 1826
BstEII GGTNACC 1 cut(s) 1589
BstF5I GGATG 6 cut(s) 32, 219, 457, 476, 1489, 1720
BstFNI CGCG 6 cut(s) 153, 189, 788, 1256, 1633, 1791
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 5 cut(s) 266, 491, 790, 844, 1256
BstMAI GTCTC 4 cut(s) 205, 313, 1238, 1483
BstMCI CGRYCG 1 cut(s) 1212
BstMWI GCNNNNNNNGC 3 cut(s) 186, 944, 1217
BstNI CCWGG 3 cut(s) 1466, 1553, 1675
BstNSI RCATGY 2 cut(s) 835, 862
BstPAI GACNNNNGTC 2 cut(s) 764, 1596
BstPI GGTNACC 1 cut(s) 1589
BstSCI CCNGG 5 cut(s) 897, 1089, 1464, 1551, 1673
BstSFI CTRYAG 1 cut(s) 1318
BstSLI GKGCMC 1 cut(s) 541
BstUI CGCG 6 cut(s) 153, 189, 788, 1256, 1633, 1791
BstV1I GCAGC 6 cut(s) 947, 1170, 1317, 1362, 1815, 1843
BstV2I GAAGAC 2 cut(s) 1358, 1388
BstX2I RGATCY 2 cut(s) 1118, 1580
BstXI CCANNNNNNTGG 2 cut(s) 610, 1435
BstYI RGATCY 2 cut(s) 1118, 1580
BstZI CGGCCG 1 cut(s) 1209
BsuRI GGCC 8 cut(s) 192, 539, 668, 1211, 1225, 1291, 1427, 1433
BtgI CCRYGG 4 cut(s) 750, 979, 1212, 1826
BtgZI GCGATG 1 cut(s) 415
BtrI CACGTC 4 cut(s) 196, 1075, 1129, 1279
BtsCI GGATG 6 cut(s) 32, 219, 457, 476, 1489, 1720
BtsI GCAGTG 1 cut(s) 616
BtsIMutI CAGTG 2 cut(s) 539, 616
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 1 cut(s) 933
CciI TCATGA 1 cut(s) 909
CfoI GCGC 5 cut(s) 266, 491, 790, 844, 1256
Cfr10I RCCGGY 2 cut(s) 844, 1067
CsiI ACCWGGT 1 cut(s) 1551
Csp6I GTAC 7 cut(s) 232, 496, 671, 736, 872, 985, 1315
CviAII CATG 9 cut(s) 229, 256, 832, 859, 910, 1045, 1342, 1528, 1827
CviQI GTAC 7 cut(s) 232, 496, 671, 736, 872, 985, 1315
DdeI CTNAG 4 cut(s) 362, 954, 1415, 1451
DinI GGCGCC 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 791
DriI GACNNNNNGTC 3 cut(s) 138, 683, 821
DseDI GACNNNNNNGTC 1 cut(s) 791
EaeI YGGCCR 3 cut(s) 190, 1209, 1425
EagI CGGCCG 1 cut(s) 1209
Eam1104I CTCTTC 3 cut(s) 350, 398, 638
Eam1105I GACNNNNNGTC 3 cut(s) 138, 683, 821
EarI CTCTTC 3 cut(s) 350, 398, 638
EciI GGCGGA 3 cut(s) 311, 915, 1300
Ecl136II GAGCTC 2 cut(s) 238, 923
EclXI CGGCCG 1 cut(s) 1209
Eco130I CCWWGG 5 cut(s) 603, 1122, 1428, 1434, 1826
Eco147I AGGCCT 2 cut(s) 1225, 1433
Eco24I GRGCYC 5 cut(s) 240, 541, 633, 925, 1826
Eco47I GGWCC 8 cut(s) 559, 613, 759, 850, 901, 1440, 1671, 1769
Eco52I CGGCCG 1 cut(s) 1209
Eco53kI GAGCTC 2 cut(s) 238, 923
Eco57I CTGAAG 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 1589
EcoICRI GAGCTC 2 cut(s) 238, 923
EcoO109I RGGNCCY 2 cut(s) 538, 559
EcoO65I GGTNACC 1 cut(s) 1589
EcoRII CCWGG 3 cut(s) 1464, 1551, 1673
EcoT14I CCWWGG 5 cut(s) 603, 1122, 1428, 1434, 1826
EcoT38I GRGCYC 5 cut(s) 240, 541, 633, 925, 1826
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 5 cut(s) 603, 1122, 1428, 1434, 1826
Esp3I CGTCTC 3 cut(s) 205, 313, 1238
FaeI CATG 9 cut(s) 232, 259, 835, 862, 913, 1048, 1345, 1531, 1830
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 236, 572, 772
FatI CATG 9 cut(s) 228, 255, 831, 858, 909, 1044, 1341, 1527, 1826
FauI CCCGC 8 cut(s) 31, 45, 232, 296, 308, 940, 986, 1133
FauNDI CATATG 1 cut(s) 1849
FbaI TGATCA 2 cut(s) 807, 1131
FblI GTMKAC 3 cut(s) 78, 351, 1388
FokI GGATG 6 cut(s) 19, 206, 444, 463, 1476, 1727
FriOI GRGCYC 5 cut(s) 240, 541, 633, 925, 1826
FspBI CTAG 3 cut(s) 59, 1622, 1773
GlaI GCGC 5 cut(s) 265, 490, 789, 843, 1255
GsaI CCCAGC 1 cut(s) 965
GsuI CTGGAG 3 cut(s) 745, 1124, 1479
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 8 cut(s) 192, 539, 668, 1211, 1225, 1291, 1427, 1433
HhaI GCGC 5 cut(s) 266, 491, 790, 844, 1256
Hin1I GRCGYC 6 cut(s) 180, 264, 381, 822, 1247, 1385
Hin1II CATG 9 cut(s) 232, 259, 835, 862, 913, 1048, 1345, 1531, 1830
Hin6I GCGC 5 cut(s) 264, 489, 788, 842, 1254
HinP1I GCGC 5 cut(s) 264, 489, 788, 842, 1254
HincII GTYRAC 3 cut(s) 352, 649, 1389
HindII GTYRAC 3 cut(s) 352, 649, 1389
HinfI GANTC 9 cut(s) 473, 956, 966, 1172, 1453, 1532, 1603, 1643, 1813
HphI GGTGA 7 cut(s) 34, 634, 985, 1093, 1210, 1601, 1808
Hpy166II GTNNAC 8 cut(s) 79, 352, 511, 616, 649, 1201, 1389, 1443
Hpy188I TCNGA 9 cut(s) 185, 472, 705, 812, 951, 1017, 1404, 1458, 1602
Hpy188III TCNNGA 9 cut(s) 524, 694, 904, 910, 1103, 1496, 1584, 1614, 1796
Hpy8I GTNNAC 8 cut(s) 79, 352, 511, 616, 649, 1201, 1389, 1443
HpyAV CCTTC 3 cut(s) 102, 493, 1294
HpyCH4III ACNGT 7 cut(s) 76, 140, 307, 646, 1055, 1357, 1477
HpyCH4IV ACGT 8 cut(s) 122, 195, 494, 765, 1074, 1128, 1278, 1385
HpyCH4V TGCA 5 cut(s) 446, 452, 1044, 1087, 1320
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 944, 1217
HpyF3I CTNAG 4 cut(s) 362, 954, 1415, 1451
HpySE526I ACGT 8 cut(s) 122, 195, 494, 765, 1074, 1128, 1278, 1385
Hsp92I GRCGYC 6 cut(s) 180, 264, 381, 822, 1247, 1385
Hsp92II CATG 9 cut(s) 232, 259, 835, 862, 913, 1048, 1345, 1531, 1830
HspAI GCGC 5 cut(s) 264, 489, 788, 842, 1254
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 559
Kpn2I TCCGGA 1 cut(s) 523
Ksp22I TGATCA 2 cut(s) 807, 1131
LguI GCTCTTC 1 cut(s) 638
Lsp1109I GCAGC 6 cut(s) 947, 1170, 1317, 1362, 1815, 1843
LweI GCATC 3 cut(s) 134, 433, 1074
MabI ACCWGGT 1 cut(s) 1551
MaeI CTAG 3 cut(s) 59, 1622, 1773
MaeII ACGT 8 cut(s) 122, 195, 494, 765, 1074, 1128, 1278, 1385
MaeIII GTNAC 2 cut(s) 823, 1589
MbiI CCGCTC 1 cut(s) 40
MboII GAAGA 6 cut(s) 337, 385, 625, 1363, 1393, 1788
MflI RGATCY 2 cut(s) 1118, 1580
MhlI GDGCHC 6 cut(s) 240, 541, 633, 925, 1548, 1826
MlsI TGGCCA 1 cut(s) 1427
MluCI AATT 4 cut(s) 779, 1336, 1398, 1562
MluNI TGGCCA 1 cut(s) 1427
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 6 cut(s) 467, 965, 1181, 1462, 1612, 1652
MmeI TCCRAC 3 cut(s) 208, 495, 1436
Mox20I TGGCCA 1 cut(s) 1427
MroI TCCGGA 1 cut(s) 523
MscI TGGCCA 1 cut(s) 1427
MseI TTAA 1 cut(s) 1858
MslI CAYNNNNRTG 1 cut(s) 830
Msp20I TGGCCA 1 cut(s) 1427
MspA1I CMGCKG 2 cut(s) 516, 938
MspR9I CCNGG 5 cut(s) 899, 1091, 1466, 1553, 1675
MvaI CCWGG 3 cut(s) 1466, 1553, 1675
MvnI CGCG 6 cut(s) 153, 189, 788, 1256, 1633, 1791
MwoI GCNNNNNNNGC 3 cut(s) 186, 944, 1217
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 899, 1091
NcoI CCATGG 1 cut(s) 1826
NdeI CATATG 1 cut(s) 1849
NlaIII CATG 9 cut(s) 232, 259, 835, 862, 913, 1048, 1345, 1531, 1830
NmeAIII GCCGAG 3 cut(s) 30, 156, 1172
NmuCI GTSAC 2 cut(s) 823, 1589
NspI RCATGY 2 cut(s) 835, 862
PagI TCATGA 1 cut(s) 909
PaqCI CACCTGC 1 cut(s) 457
PceI AGGCCT 2 cut(s) 1225, 1433
PciI ACATGT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 638
PcsI WCGNNNNNNNCGW 6 cut(s) 201, 438, 666, 872, 1209, 1253
PfeI GAWTC 3 cut(s) 966, 1532, 1813
Pfl23II CGTACG 2 cut(s) 495, 735
PflFI GACNNNGTC 1 cut(s) 955
PflMI CCANNNNNTGG 1 cut(s) 723
PleI GAGTC 6 cut(s) 467, 964, 1180, 1461, 1611, 1651
PluTI GGCGCC 1 cut(s) 267
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 6 cut(s) 467, 964, 1180, 1461, 1611, 1651
Ppu21I YACGTR 2 cut(s) 123, 495
PpuMI RGGWCCY 1 cut(s) 559
PscI ACATGT 1 cut(s) 831
PshAI GACNNNNGTC 2 cut(s) 764, 1596
Psp124BI GAGCTC 2 cut(s) 240, 925
Psp5II RGGWCCY 1 cut(s) 559
Psp6I CCWGG 3 cut(s) 1464, 1551, 1673
PspCI CACGTG 1 cut(s) 123
PspEI GGTNACC 1 cut(s) 1589
PspFI CCCAGC 1 cut(s) 961
PspGI CCWGG 3 cut(s) 1464, 1551, 1673
PspLI CGTACG 2 cut(s) 495, 735
PspOMI GGGCCC 1 cut(s) 537
PspPPI RGGWCCY 1 cut(s) 559
PstI CTGCAG 1 cut(s) 1322
PsuI RGATCY 2 cut(s) 1118, 1580
PsyI GACNNNGTC 1 cut(s) 955
RsaI GTAC 7 cut(s) 233, 497, 672, 737, 873, 986, 1316
RsaNI GTAC 7 cut(s) 232, 496, 671, 736, 872, 985, 1315
RseI CAYNNNNRTG 1 cut(s) 830
SacI GAGCTC 2 cut(s) 240, 925
SalI GTCGAC 2 cut(s) 350, 1387
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 1 cut(s) 1858
ScaI AGTACT 1 cut(s) 1316
SchI GAGTC 6 cut(s) 467, 965, 1181, 1462, 1612, 1652
ScrFI CCNGG 5 cut(s) 899, 1091, 1466, 1553, 1675
SduI GDGCHC 6 cut(s) 240, 541, 633, 925, 1548, 1826
SexAI ACCWGGT 1 cut(s) 1551
SfaNI GCATC 3 cut(s) 134, 433, 1074
SfcI CTRYAG 1 cut(s) 1318
SfoI GGCGCC 1 cut(s) 265
SgrAI CRCCGGYG 2 cut(s) 844, 1067
SgrDI CGTCGACG 1 cut(s) 1387
SinI GGWCC 8 cut(s) 559, 613, 759, 850, 901, 1440, 1671, 1769
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 1 cut(s) 1153
SmoI CTYRAG 1 cut(s) 1153
Sse9I AATT 4 cut(s) 779, 1336, 1398, 1562
SseBI AGGCCT 2 cut(s) 1225, 1433
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 3 cut(s) 59, 1622, 1773
SstI GAGCTC 2 cut(s) 240, 925
StuI AGGCCT 2 cut(s) 1225, 1433
StyD4I CCNGG 5 cut(s) 897, 1089, 1464, 1551, 1673
StyI CCWWGG 5 cut(s) 603, 1122, 1428, 1434, 1826
TaaI ACNGT 7 cut(s) 76, 140, 307, 646, 1055, 1357, 1477
TaiI ACGT 8 cut(s) 125, 198, 497, 768, 1077, 1131, 1281, 1388
TaqII GACCGA 1 cut(s) 78
TasI AATT 4 cut(s) 779, 1336, 1398, 1562
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 6 cut(s) 71, 192, 519, 749, 931, 1211
TfiI GAWTC 3 cut(s) 966, 1532, 1813
Tru1I TTAA 1 cut(s) 1858
Tru9I TTAA 1 cut(s) 1858
TscAI CASTG 2 cut(s) 539, 623
TseFI GTSAC 2 cut(s) 823, 1589
TseI GCWGC 6 cut(s) 935, 1158, 1330, 1375, 1803, 1831
Tsp45I GTSAC 2 cut(s) 823, 1589
TspDTI ATGAA 4 cut(s) 625, 1061, 1330, 1555
TspGWI ACGGA 3 cut(s) 193, 1002, 1310
TspRI CASTG 2 cut(s) 539, 623
Tth111I GACNNNGTC 1 cut(s) 955
Van91I CCANNNNNTGG 1 cut(s) 723
VpaK11BI GGWCC 8 cut(s) 559, 613, 759, 850, 901, 1440, 1671, 1769
XapI RAATTY 4 cut(s) 779, 1336, 1398, 1562
XceI RCATGY 2 cut(s) 835, 862
XmiI GTMKAC 3 cut(s) 78, 351, 1388
XspI CTAG 3 cut(s) 59, 1622, 1773
ZraI GACGTC 1 cut(s) 1386
ZrmI AGTACT 1 cut(s) 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.