Rw3G025200

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000677
Physical Location & Seq
Reverse (-)
33262365 .. 33264116
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025200.1

Sequence Viewer

Length: 1752 bp
ATGGAAGAGCTGAAGCCAACCCCCGCCAACTCATCCCCGCTCACCCCACTCGGCTTCCTAGACCGAGCCGCCACCGTCTACGGCGACTGCCCTTCTGTCATCTACGAACACACCACCTACACTTGGAGCCAGACCCACCGTCGATGCCTCCGCGTCGCTTCCTCCATCTCCTCCCTCGGCATCAGACGCGGCCACGTCGTCTCCGTCCTCTCCCCCAACATCCCCGCCATGTACGAGCTCCACTTCGCCGTCCCCATGTCCGGCGCCGTCCTCAACACCATCAACACCCGCCTCGACACCTGCACCGTCTCCCTCCTCCTCCGCCACAGCCAATCCAAGCTCCTCTTCGTCGACCACACCCTCAGCTCCCTCGTCCTCGACGCCCTCCATCTCTTCCCTCCCAACACCCCCATCCCCCTCCTCGTCCTCATCGCCGACTTCGATGCACCCGCACCCCATCCCAACTCCGACTCATCCCCGTTCTGCGCCACGTACGAGGAAATGGTGGACAGCGGCGATCCGGACTTCCAGTGGACCCTACCGGAAGACGAGTGGGACCCGATGACGCTGAACTACACTTCCGGCACTACTTCCTCCCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCATCAACTCTCTCCTCGACTGGGCAGTACCCAGACAGCCGGTCTACCTATGGACGCTTCCGATGTTCCACGCCAACGGCTGGAGCTACCCGTACGGGATCGCCGCCGTCGGTGGGACCAACGTTTGCGTCCGCAAATTCGACGCCCATGTCATCTACGACTTGATCAGACGACACGGCGTCACTCACATGTGCGGTGCGCCGGTGGTCCTCAACATGCTGACGAATGTACCGAACAACGAGCCTTTGAGGAACCCGGTCCAGATCATGACCGCCGGAGCTCCGCCACCTGCAGCGGTTCTGTCTCGGACTGAGTCTCTGGGATTCGTGGTGAGCCATGGGTACGGGTTGACAGAGACGGCAGGGCCGGTGGTGTCGTGCGCGTGGAAGCCCGGGTGGAATAAGTTGCCGGCGACGGAGAGGGCGAGGCTGAAGTCGAGGCAGGGAGTGAGGATGGTGGCCATGACCCACGTCGACGTGGTGGATCCGAATACGGGTGCGCCGGTTAAACGCGACGGTTCATCGCTCGGGGAGGTGGTTTTCAGAGGCGGGTCTGTGATGTTGGGTTACCTGAAAGACCCAAAAGGCACGGCTAGTTGCATGAAAGACGGTTTGTTTTATACCGGCGACGTGGCAGTGATGCACCCGGACGGGTATCTGGAGATCAAGGATCGATCTAAGGACGTGATCATTAGCGGGGGAGAGAACGTGAGCAGCGTGGAGGTCGAGTCGGTTCTCTACACCAACGCGGCGGTGAACGAGGCGGCAGTGGTGGCTAGGCCTGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTTGTGAGCCTGAAAGCTGACGTGGCGCAGAGTCCGACGGAGAAGGAGATAATAGAATATTGCAGGGGGAAGTTGCCGCATTACATGGTGCCGAAGACGGTGGTGTTTAAGGAGGAGCTGCCGAAGACTTCGACGGGGAAGATTCAGAAGTTCGTGCTGAGAGAGATGGCGGCCCAGTGTTACTTGGTTCGCATGTCGTTGCGGAATGGGTACACGGTACGAGTATGCAGTAGGCCAGCCTCTATGATACGCAGTACGCGAGTCTCTATGATACACAGTACATCAATCTCTATGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

583

Amino Acids

63.9

Weight (kDa)

7.02

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 404 2.1e-75 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 2.9e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 308, 937
AasI GACNNNNNNGTC 1 cut(s) 788
Acc36I ACCTGC 2 cut(s) 308, 937
AccB1I GGYRCC 2 cut(s) 263, 1540
AccB7I CCANNNNNTGG 1 cut(s) 720
AccBSI CCGCTC 1 cut(s) 40
AccI GTMKAC 4 cut(s) 78, 351, 684, 1113
AccII CGCG 7 cut(s) 153, 189, 1022, 1152, 1388, 1451, 1711
AccIII TCCGGA 1 cut(s) 520
AclI AACGTT 1 cut(s) 762
AclWI GGATC 5 cut(s) 512, 746, 1118, 1131, 1317
AcoI YGGCCR 2 cut(s) 190, 1098
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 2 cut(s) 32, 1091
AcyI GRCGYC 5 cut(s) 264, 381, 783, 819, 1442
AfiI CCNNNNNNNGG 6 cut(s) 123, 260, 661, 720, 753, 1133
AflIII ACRYGT 1 cut(s) 828
AhdI GACNNNNNGTC 3 cut(s) 138, 680, 818
AjiI CACGTC 6 cut(s) 196, 1111, 1117, 1270, 1324, 1474
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AloI GAACNNNNNNTCC 2 cut(s) 563, 595
AluBI AGCT 8 cut(s) 10, 238, 340, 366, 726, 920, 1469, 1570
AluI AGCT 8 cut(s) 10, 238, 340, 366, 726, 920, 1469, 1570
Alw21I GWGCWC 2 cut(s) 240, 922
Alw26I GTCTC 7 cut(s) 205, 313, 948, 960, 989, 1433, 1720
AlwI GGATC 5 cut(s) 512, 746, 1118, 1131, 1317
Ama87I CYCGRG 2 cut(s) 1031, 1166
Aor13HI TCCGGA 1 cut(s) 520
AoxI GGCC 6 cut(s) 190, 1004, 1098, 1418, 1623, 1685
ApeKI GCWGC 3 cut(s) 932, 1353, 1570
ApoI RAATTY 1 cut(s) 776
AspLEI GCGC 7 cut(s) 266, 488, 841, 1022, 1141, 1451, 1480
AspS9I GGNCC 8 cut(s) 534, 556, 610, 756, 847, 898, 1004, 1624
AsuC2I CCSGG 4 cut(s) 896, 1032, 1033, 1286
AsuHPI GGTGA 4 cut(s) 34, 631, 982, 1405
AvaI CYCGRG 2 cut(s) 1031, 1166
AvaII GGWCC 6 cut(s) 534, 556, 610, 756, 847, 898
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BalI TGGCCA 1 cut(s) 1100
BamHI GGATCC 1 cut(s) 1123
BanI GGYRCC 2 cut(s) 263, 1540
BanII GRGCYC 3 cut(s) 240, 630, 922
BbsI GAAGAC 3 cut(s) 552, 1553, 1583
Bbv12I GWGCWC 2 cut(s) 240, 922
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 3 cut(s) 944, 1365, 1557
BccI CCATC 8 cut(s) 173, 287, 396, 419, 465, 650, 1087, 1612
BceAI ACGGC 9 cut(s) 97, 233, 251, 731, 733, 832, 1014, 1245, 1429
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BclI TGATCA 2 cut(s) 804, 1326
BcnI CCSGG 4 cut(s) 896, 1032, 1033, 1286
BcoDI GTCTC 7 cut(s) 205, 313, 948, 960, 989, 1433, 1720
BfaI CTAG 3 cut(s) 59, 1233, 1416
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 267
BfuAI ACCTGC 2 cut(s) 308, 937
Bme1390I CCNGG 4 cut(s) 896, 1032, 1033, 1286
Bme18I GGWCC 6 cut(s) 534, 556, 610, 756, 847, 898
BmeRI GACNNNNNGTC 3 cut(s) 138, 680, 818
BmeT110I CYCGRG 2 cut(s) 1031, 1166
BmgBI CACGTC 6 cut(s) 196, 1111, 1117, 1270, 1324, 1474
BmgT120I GGNCC 8 cut(s) 534, 556, 610, 756, 847, 898, 1004, 1624
BmiI GGNNCC 9 cut(s) 128, 265, 536, 557, 558, 757, 893, 1125, 1542
BmrFI CCNGG 4 cut(s) 896, 1032, 1033, 1286
BmrI ACTGGG 2 cut(s) 670, 1621
BmsI GCATC 4 cut(s) 134, 189, 433, 1269
BmuI ACTGGG 2 cut(s) 670, 1621
BoxI GACNNNNGTC 1 cut(s) 1109
BpiI GAAGAC 3 cut(s) 552, 1553, 1583
BpmI CTGGAG 2 cut(s) 742, 1319
Bpu10I CCTNAGC 1 cut(s) 362
BpuMI CCSGG 4 cut(s) 896, 1032, 1033, 1286
Bsa29I ATCGAT 1 cut(s) 1312
BsaAI YACGTR 1 cut(s) 492
BsaHI GRCGYC 5 cut(s) 264, 381, 783, 819, 1442
BsaJI CCNNGG 4 cut(s) 175, 600, 976, 1031
BsaWI WCCGGW 2 cut(s) 520, 541
BsaXI ACNNNNNCTCC 8 cut(s) 185, 215, 1332, 1352, 1362, 1382, 1430, 1460
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 260, 661, 720, 753, 1133
Bse118I RCCGGY 6 cut(s) 679, 841, 1006, 1048, 1141, 1262
Bse1I ACTGG 3 cut(s) 529, 665, 1627
BseAI TCCGGA 1 cut(s) 520
BseCI ATCGAT 1 cut(s) 1312
BseDI CCNNGG 4 cut(s) 175, 600, 976, 1031
BseGI GGATG 6 cut(s) 32, 219, 411, 457, 473, 1098
BseLI CCNNNNNNNGG 6 cut(s) 123, 260, 661, 720, 753, 1133
BseMII CTCAG 3 cut(s) 376, 942, 1601
BseNI ACTGG 3 cut(s) 529, 665, 1627
BseRI GAGGAG 7 cut(s) 160, 305, 308, 332, 410, 644, 1580
BseXI GCAGC 3 cut(s) 944, 1365, 1557
BsgI GTGCAG 1 cut(s) 286
Bsh1236I CGCG 7 cut(s) 153, 189, 1022, 1152, 1388, 1451, 1711
BshFI GGCC 6 cut(s) 192, 1006, 1100, 1420, 1625, 1687
BshNI GGYRCC 2 cut(s) 263, 1540
BshVI ATCGAT 1 cut(s) 1312
BsiHKAI GWGCWC 2 cut(s) 240, 922
BsiHKCI CYCGRG 2 cut(s) 1031, 1166
BsiWI CGTACG 2 cut(s) 492, 732
BslFI GGGAC 3 cut(s) 236, 569, 769
BslI CCNNNNNNNGG 6 cut(s) 123, 260, 661, 720, 753, 1133
BsmAI GTCTC 7 cut(s) 205, 313, 948, 960, 989, 1433, 1720
BsmBI CGTCTC 4 cut(s) 205, 313, 989, 1433
BsmFI GGGAC 3 cut(s) 236, 569, 769
BsnI GGCC 6 cut(s) 192, 1006, 1100, 1420, 1625, 1687
BsoBI CYCGRG 2 cut(s) 1031, 1166
Bsp1286I GDGCHC 3 cut(s) 240, 630, 922
Bsp13I TCCGGA 1 cut(s) 520
Bsp143I GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
Bsp19I CCATGG 1 cut(s) 976
BspANI GGCC 6 cut(s) 192, 1006, 1100, 1420, 1625, 1687
BspCNI CTCAG 3 cut(s) 375, 943, 1602
BspDI ATCGAT 1 cut(s) 1312
BspEI TCCGGA 1 cut(s) 520
BspFNI CGCG 7 cut(s) 153, 189, 1022, 1152, 1388, 1451, 1711
BspHI TCATGA 1 cut(s) 906
BspLI GGNNCC 9 cut(s) 128, 265, 536, 557, 558, 757, 893, 1125, 1542
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 2 cut(s) 308, 937
BspPI GGATC 5 cut(s) 512, 746, 1118, 1131, 1317
BspQI GCTCTTC 1 cut(s) 635
BspT107I GGYRCC 2 cut(s) 263, 1540
BsrBI CCGCTC 1 cut(s) 40
BsrFI RCCGGY 6 cut(s) 679, 841, 1006, 1048, 1141, 1262
BsrI ACTGG 3 cut(s) 529, 665, 1627
BssAI RCCGGY 6 cut(s) 679, 841, 1006, 1048, 1141, 1262
BssECI CCNNGG 4 cut(s) 175, 600, 976, 1031
BssMI GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
BssNI GRCGYC 5 cut(s) 264, 381, 783, 819, 1442
BssT1I CCWWGG 2 cut(s) 600, 976
Bst4CI ACNGT 8 cut(s) 76, 140, 307, 1157, 1250, 1552, 1669, 1730
Bst6I CTCTTC 3 cut(s) 350, 398, 635
BstACI GRCGYC 5 cut(s) 264, 381, 783, 819, 1442
BstBAI YACGTR 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 1050, 1689
BstDEI CTNAG 4 cut(s) 362, 951, 1317, 1610
BstDSI CCRYGG 1 cut(s) 976
BstEII GGTNACC 1 cut(s) 1205
BstF5I GGATG 6 cut(s) 32, 219, 411, 457, 473, 1098
BstFNI CGCG 7 cut(s) 153, 189, 1022, 1152, 1388, 1451, 1711
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 7 cut(s) 266, 488, 841, 1022, 1141, 1451, 1480
BstKTI GATC 9 cut(s) 520, 741, 807, 906, 1126, 1305, 1312, 1316, 1329
BstMAI GTCTC 7 cut(s) 205, 313, 948, 960, 989, 1433, 1720
BstMBI GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
BstMWI GCNNNNNNNGC 3 cut(s) 186, 1412, 1475
BstNSI RCATGY 3 cut(s) 832, 859, 1648
BstPAI GACNNNNGTC 1 cut(s) 1109
BstPI GGTNACC 1 cut(s) 1205
BstSCI CCNGG 4 cut(s) 894, 1030, 1031, 1284
BstSFI CTRYAG 1 cut(s) 930
BstUI CGCG 7 cut(s) 153, 189, 1022, 1152, 1388, 1451, 1711
BstV1I GCAGC 3 cut(s) 944, 1365, 1557
BstV2I GAAGAC 3 cut(s) 552, 1553, 1583
BstX2I RGATCY 1 cut(s) 1123
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 1 cut(s) 1123
Bsu15I ATCGAT 1 cut(s) 1312
BsuRI GGCC 6 cut(s) 192, 1006, 1100, 1420, 1625, 1687
BsuTUI ATCGAT 1 cut(s) 1312
BtgI CCRYGG 1 cut(s) 976
BtgZI GCGATG 2 cut(s) 415, 1146
BtrI CACGTC 6 cut(s) 196, 1111, 1117, 1270, 1324, 1474
BtsCI GGATG 6 cut(s) 32, 219, 411, 457, 473, 1098
BtsI GCAGTG 3 cut(s) 613, 1281, 1413
BtsIMutI CAGTG 5 cut(s) 536, 613, 1281, 1413, 1634
BveI ACCTGC 2 cut(s) 308, 937
Cac8I GCNNGC 2 cut(s) 1050, 1689
CciI TCATGA 1 cut(s) 906
CfoI GCGC 7 cut(s) 266, 488, 841, 1022, 1141, 1451, 1480
Cfr10I RCCGGY 6 cut(s) 679, 841, 1006, 1048, 1141, 1262
Cfr13I GGNCC 8 cut(s) 534, 556, 610, 756, 847, 898, 1004, 1624
Cfr9I CCCGGG 1 cut(s) 1031
ClaI ATCGAT 1 cut(s) 1312
CseI GACGC 9 cut(s) 142, 195, 389, 574, 703, 757, 791, 808, 1450
DdeI CTNAG 4 cut(s) 362, 951, 1317, 1610
DinI GGCGCC 1 cut(s) 265
DpnI GATC 9 cut(s) 519, 740, 806, 905, 1125, 1304, 1311, 1315, 1328
DpnII GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
DrdI GACNNNNNNGTC 1 cut(s) 788
DriI GACNNNNNGTC 3 cut(s) 138, 680, 818
DseDI GACNNNNNNGTC 1 cut(s) 788
EaeI YGGCCR 2 cut(s) 190, 1098
Eam1104I CTCTTC 3 cut(s) 350, 398, 635
Eam1105I GACNNNNNGTC 3 cut(s) 138, 680, 818
EarI CTCTTC 3 cut(s) 350, 398, 635
EciI GGCGGA 2 cut(s) 311, 912
Ecl136II GAGCTC 2 cut(s) 238, 920
Eco130I CCWWGG 2 cut(s) 600, 976
Eco147I AGGCCT 1 cut(s) 1420
Eco24I GRGCYC 3 cut(s) 240, 630, 922
Eco47I GGWCC 6 cut(s) 534, 556, 610, 756, 847, 898
Eco53kI GAGCTC 2 cut(s) 238, 920
Eco57I CTGAAG 2 cut(s) 32, 1091
Eco88I CYCGRG 2 cut(s) 1031, 1166
Eco91I GGTNACC 1 cut(s) 1205
EcoICRI GAGCTC 2 cut(s) 238, 920
EcoO109I RGGNCCY 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 1205
EcoT14I CCWWGG 2 cut(s) 600, 976
EcoT38I GRGCYC 3 cut(s) 240, 630, 922
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 600, 976
Esp3I CGTCTC 4 cut(s) 205, 313, 989, 1433
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 3 cut(s) 236, 569, 769
FauI CCCGC 7 cut(s) 31, 45, 232, 296, 457, 1181, 1328
FbaI TGATCA 2 cut(s) 804, 1326
FblI GTMKAC 4 cut(s) 78, 351, 684, 1113
FokI GGATG 6 cut(s) 19, 206, 398, 444, 460, 1105
FriOI GRGCYC 3 cut(s) 240, 630, 922
FspBI CTAG 3 cut(s) 59, 1233, 1416
GlaI GCGC 7 cut(s) 265, 487, 840, 1021, 1140, 1450, 1479
GsuI CTGGAG 2 cut(s) 742, 1319
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 6 cut(s) 192, 1006, 1100, 1420, 1625, 1687
HgaI GACGC 9 cut(s) 142, 195, 389, 574, 703, 757, 791, 808, 1450
HhaI GCGC 7 cut(s) 266, 488, 841, 1022, 1141, 1451, 1480
Hin1I GRCGYC 5 cut(s) 264, 381, 783, 819, 1442
Hin6I GCGC 7 cut(s) 264, 486, 839, 1020, 1139, 1449, 1478
HinP1I GCGC 7 cut(s) 264, 486, 839, 1020, 1139, 1449, 1478
HincII GTYRAC 3 cut(s) 352, 990, 1114
HindII GTYRAC 3 cut(s) 352, 990, 1114
HinfI GANTC 7 cut(s) 470, 953, 963, 1367, 1483, 1594, 1713
HphI GGTGA 4 cut(s) 34, 631, 982, 1405
Hpy188I TCNGA 9 cut(s) 185, 469, 702, 809, 948, 1128, 1184, 1488, 1599
Hpy188III TCNNGA 4 cut(s) 521, 901, 907, 1298
HpyAV CCTTC 2 cut(s) 102, 1489
HpyCH4III ACNGT 8 cut(s) 76, 140, 307, 1157, 1250, 1552, 1669, 1730
HpyCH4IV ACGT 9 cut(s) 195, 491, 762, 1110, 1116, 1269, 1323, 1347, 1473
HpyCH4V TGCA 7 cut(s) 303, 446, 932, 1239, 1282, 1515, 1680
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 1412, 1475
HpyF3I CTNAG 4 cut(s) 362, 951, 1317, 1610
HpySE526I ACGT 9 cut(s) 195, 491, 762, 1110, 1116, 1269, 1323, 1347, 1473
Hsp92I GRCGYC 5 cut(s) 264, 381, 783, 819, 1442
HspAI GCGC 7 cut(s) 264, 486, 839, 1020, 1139, 1449, 1478
KasI GGCGCC 1 cut(s) 263
KflI GGGWCCC 1 cut(s) 556
Kpn2I TCCGGA 1 cut(s) 520
KroI GCCGGC 1 cut(s) 1048
KroNI GCCGGC 1 cut(s) 1050
Ksp22I TGATCA 2 cut(s) 804, 1326
Kzo9I GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
LguI GCTCTTC 1 cut(s) 635
LmnI GCTCC 8 cut(s) 126, 243, 345, 371, 723, 917, 925, 1567
Lsp1109I GCAGC 3 cut(s) 944, 1365, 1557
LweI GCATC 4 cut(s) 134, 189, 433, 1269
MaeI CTAG 3 cut(s) 59, 1233, 1416
MaeII ACGT 9 cut(s) 195, 491, 762, 1110, 1116, 1269, 1323, 1347, 1473
MaeIII GTNAC 3 cut(s) 820, 1205, 1631
MalI GATC 9 cut(s) 519, 740, 806, 905, 1125, 1304, 1311, 1315, 1328
MbiI CCGCTC 1 cut(s) 40
MboI GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
MboII GAAGA 8 cut(s) 17, 337, 385, 557, 622, 1558, 1588, 1603
MflI RGATCY 1 cut(s) 1123
MhlI GDGCHC 3 cut(s) 240, 630, 922
MlsI TGGCCA 1 cut(s) 1100
MluCI AATT 1 cut(s) 776
MluNI TGGCCA 1 cut(s) 1100
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 5 cut(s) 464, 962, 1376, 1492, 1722
MmeI TCCRAC 2 cut(s) 492, 1511
Mox20I TGGCCA 1 cut(s) 1100
MroI TCCGGA 1 cut(s) 520
MroNI GCCGGC 1 cut(s) 1048
MscI TGGCCA 1 cut(s) 1100
MseI TTAA 2 cut(s) 1146, 1560
MslI CAYNNNNRTG 1 cut(s) 827
Msp20I TGGCCA 1 cut(s) 1100
MspA1I CMGCKG 2 cut(s) 513, 935
MspR9I CCNGG 4 cut(s) 896, 1032, 1033, 1286
MvnI CGCG 7 cut(s) 153, 189, 1022, 1152, 1388, 1451, 1711
MwoI GCNNNNNNNGC 3 cut(s) 186, 1412, 1475
NaeI GCCGGC 1 cut(s) 1050
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 4 cut(s) 896, 1032, 1033, 1286
NcoI CCATGG 1 cut(s) 976
NdeII GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
NgoMIV GCCGGC 1 cut(s) 1048
NlaIV GGNNCC 9 cut(s) 128, 265, 536, 557, 558, 757, 893, 1125, 1542
NmeAIII GCCGAG 2 cut(s) 30, 156
NmuCI GTSAC 1 cut(s) 820
NspI RCATGY 3 cut(s) 832, 859, 1648
PagI TCATGA 1 cut(s) 906
PaqCI CACCTGC 2 cut(s) 308, 937
PceI AGGCCT 1 cut(s) 1420
PciI ACATGT 1 cut(s) 828
PciSI GCTCTTC 1 cut(s) 635
PcsI WCGNNNNNNNCGW 7 cut(s) 201, 438, 869, 1061, 1353, 1448, 1708
PdiI GCCGGC 1 cut(s) 1050
PfeI GAWTC 2 cut(s) 963, 1594
Pfl23II CGTACG 2 cut(s) 492, 732
PflFI GACNNNGTC 1 cut(s) 952
PflMI CCANNNNNTGG 1 cut(s) 720
PleI GAGTC 5 cut(s) 464, 961, 1375, 1491, 1721
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 5 cut(s) 464, 961, 1375, 1491, 1721
Ppu21I YACGTR 1 cut(s) 492
PpuMI RGGWCCY 1 cut(s) 556
PscI ACATGT 1 cut(s) 828
PshAI GACNNNNGTC 1 cut(s) 1109
Psp124BI GAGCTC 2 cut(s) 240, 922
Psp1406I AACGTT 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 556
PspEI GGTNACC 1 cut(s) 1205
PspLI CGTACG 2 cut(s) 492, 732
PspN4I GGNNCC 9 cut(s) 128, 265, 536, 557, 558, 757, 893, 1125, 1542
PspPI GGNCC 8 cut(s) 534, 556, 610, 756, 847, 898, 1004, 1624
PspPPI RGGWCCY 1 cut(s) 556
PstI CTGCAG 1 cut(s) 934
PsuI RGATCY 1 cut(s) 1123
PsyI GACNNNGTC 1 cut(s) 952
RseI CAYNNNNRTG 1 cut(s) 827
SacI GAGCTC 2 cut(s) 240, 922
SalI GTCGAC 2 cut(s) 350, 1112
SapI GCTCTTC 1 cut(s) 635
SaqAI TTAA 2 cut(s) 1146, 1560
Sau3AI GATC 9 cut(s) 517, 738, 804, 903, 1123, 1302, 1309, 1313, 1326
Sau96I GGNCC 8 cut(s) 534, 556, 610, 756, 847, 898, 1004, 1624
SchI GAGTC 5 cut(s) 464, 962, 1376, 1492, 1722
ScrFI CCNGG 4 cut(s) 896, 1032, 1033, 1286
SduI GDGCHC 3 cut(s) 240, 630, 922
SfaNI GCATC 4 cut(s) 134, 189, 433, 1269
SfcI CTRYAG 1 cut(s) 930
SfoI GGCGCC 1 cut(s) 265
SgrAI CRCCGGYG 1 cut(s) 841
SgrDI CGTCGACG 1 cut(s) 1112
SinI GGWCC 6 cut(s) 534, 556, 610, 756, 847, 898
SmaI CCCGGG 1 cut(s) 1033
SmiMI CAYNNNNRTG 1 cut(s) 827
Sse9I AATT 1 cut(s) 776
SseBI AGGCCT 1 cut(s) 1420
SspDI GGCGCC 1 cut(s) 263
SspI AATATT 1 cut(s) 1511
SspMI CTAG 3 cut(s) 59, 1233, 1416
SstI GAGCTC 2 cut(s) 240, 922
StuI AGGCCT 1 cut(s) 1420
StyD4I CCNGG 4 cut(s) 894, 1030, 1031, 1284
StyI CCWWGG 2 cut(s) 600, 976
TaaI ACNGT 8 cut(s) 76, 140, 307, 1157, 1250, 1552, 1669, 1730
TaiI ACGT 9 cut(s) 198, 494, 765, 1113, 1119, 1272, 1326, 1350, 1476
TaqII GACCGA 1 cut(s) 78
TasI AATT 1 cut(s) 776
TatI WGTACW 1 cut(s) 1730
TauI GCSGC 8 cut(s) 71, 192, 516, 746, 1391, 1406, 1531, 1625
TfiI GAWTC 2 cut(s) 963, 1594
Tru1I TTAA 2 cut(s) 1146, 1560
Tru9I TTAA 2 cut(s) 1146, 1560
TscAI CASTG 5 cut(s) 536, 620, 1281, 1413, 1634
TseFI GTSAC 1 cut(s) 820
TseI GCWGC 3 cut(s) 932, 1353, 1570
Tsp45I GTSAC 1 cut(s) 820
TspDTI ATGAA 3 cut(s) 622, 1149, 1256
TspGWI ACGGA 3 cut(s) 193, 1070, 1505
TspMI CCCGGG 1 cut(s) 1031
TspRI CASTG 5 cut(s) 536, 620, 1281, 1413, 1634
Tth111I GACNNNGTC 1 cut(s) 952
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 6 cut(s) 534, 556, 610, 756, 847, 898
XapI RAATTY 1 cut(s) 776
XceI RCATGY 3 cut(s) 832, 859, 1648
XmaI CCCGGG 1 cut(s) 1031
XmiI GTMKAC 4 cut(s) 78, 351, 684, 1113
XspI CTAG 3 cut(s) 59, 1233, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.