Rmu_sc0005285.1_g000017

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005285.1
OG0000677
Physical Location & Seq
Forward (+)
73633 .. 74544
912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005285.1_g000017.1.cds

Sequence Viewer

Length: 912 bp
atggaccagctgaaacctagttctgcaaacctctgccctcaaactcccttggacttcttagaccgagcagcgatcgtctacggcgactgcccctccatcatctacaactccaccaccacctacacctggtcccaaacccaccgtcgatgtctccagctggcttcctccatctccaccctcgtcaagaaatcccaggtcgtctccgtggtgtcccccaacactcccgccatgtacgaactccacttcgctgtccccatgtcgggagccgtcctcaacaacatcaacacccgcctcgacgcccacaccgtctccgtgctcctccaacacagcgaatccaagctcgtcttcgtcgacttcctctccctcaacctcgtcctccaagccctctccttgttcccctctcacatccccaccccacctctcgtcctcatcaacgatcatcatcaaccctcctcaacctccagtaccgttgacttcatagcggacacgtaccacaacatggtggccaaaggcgatcccgggttcgtttgggtccggccggcgagcgagtgggacccaatggtgctgaactacacttcagggacaacgtcagctccgaaaggtgcggtccactgccacagagctctcttcaacgtgagtctcgtttcaatcatcgactggtcggttcctaaaaattgtgtttatctgtggaccctaccgatgttccatgcaaatgggtggtgcttcccgtgggccattgccgccgtgggggggaccaacatctgccttcgcaagttcgacggtcctataatctacgatctgatccagaaacacagagtgactcatatgtgcgccgcgcctgtcgttctcaacatgctggtcaaccacccaagaaccgaccggcttccgaaccctggaggacggttggaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

303

Amino Acids

33.51

Weight (kDa)

7.68

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 499
AccI GTMKAC 2 cut(s) 78, 351
AccII CGCG 1 cut(s) 836
AciI CCGC 6 cut(s) 225, 289, 482, 605, 741, 834
AclWI GGATC 2 cut(s) 509, 796
AcoI YGGCCR 2 cut(s) 504, 536
AcuI CTGAAG 1 cut(s) 561
AcyI GRCGYC 1 cut(s) 297
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 3 cut(s) 233, 466, 491
AfiI CCNNNNNNNGG 7 cut(s) 126, 259, 260, 499, 747, 750, 893
AflIII ACRYGT 1 cut(s) 486
AgsI TTSAA 2 cut(s) 631, 648
AjnI CCWGG 3 cut(s) 125, 192, 892
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 5 cut(s) 10, 157, 340, 593, 623
AluI AGCT 5 cut(s) 10, 157, 340, 593, 623
Alw21I GWGCWC 2 cut(s) 318, 625
Alw26I GTCTC 4 cut(s) 155, 205, 313, 644
AlwI GGATC 2 cut(s) 509, 796
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 3 cut(s) 504, 536, 732
ApeKI GCWGC 1 cut(s) 68
ArsI GACNNNNNNTTYG 2 cut(s) 127, 159
AspLEI GCGC 2 cut(s) 833, 838
AspS9I GGNCC 9 cut(s) 4, 129, 532, 553, 607, 690, 732, 753, 782
AsuC2I CCSGG 2 cut(s) 519, 520
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 8 cut(s) 4, 129, 532, 553, 607, 690, 753, 782
BalI TGGCCA 1 cut(s) 506
BanII GRGCYC 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 2 cut(s) 318, 625
BbvI GCAGC 1 cut(s) 80
BccI CCATC 2 cut(s) 104, 176
BceAI ACGGC 3 cut(s) 97, 251, 728
BciT130I CCWGG 3 cut(s) 127, 194, 894
BcnI CCSGG 2 cut(s) 519, 520
BcoDI GTCTC 4 cut(s) 155, 205, 313, 644
BfaI CTAG 1 cut(s) 18
BisI GCNGC 3 cut(s) 69, 741, 834
BlsI GCNGC 3 cut(s) 70, 742, 835
Bme1390I CCNGG 5 cut(s) 127, 194, 519, 520, 894
Bme18I GGWCC 8 cut(s) 4, 129, 532, 553, 607, 690, 753, 782
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 9 cut(s) 4, 129, 532, 553, 607, 690, 732, 753, 782
BmiI GGNNCC 8 cut(s) 131, 265, 533, 554, 555, 666, 692, 754
BmrFI CCNGG 5 cut(s) 127, 194, 519, 520, 894
BpiI GAAGAC 1 cut(s) 337
BplI GAGNNNNNCTC 2 cut(s) 255, 287
BpmI CTGGAG 2 cut(s) 137, 445
BpuMI CCSGG 2 cut(s) 519, 520
BsaAI YACGTR 1 cut(s) 489
BsaBI GATNNNNATC 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 7 cut(s) 48, 192, 204, 518, 728, 744, 892
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 293, 323, 344, 374, 577, 607
Bsc4I CCNNNNNNNGG 7 cut(s) 126, 259, 260, 499, 747, 750, 893
Bse118I RCCGGY 2 cut(s) 538, 879
Bse1I ACTGG 2 cut(s) 462, 662
Bse3DI GCAATG 1 cut(s) 735
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 3 cut(s) 127, 194, 894
BseDI CCNNGG 7 cut(s) 48, 192, 204, 518, 728, 744, 892
BseGI GGATG 1 cut(s) 405
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 7 cut(s) 126, 259, 260, 499, 747, 750, 893
BseMI GCAATG 1 cut(s) 735
BseNI ACTGG 2 cut(s) 462, 662
BseRI GAGGAG 2 cut(s) 308, 442
BseX3I CGGCCG 1 cut(s) 536
BseXI GCAGC 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 836
Bsh1285I CGRYCG 3 cut(s) 75, 539, 880
BshFI GGCC 3 cut(s) 506, 538, 734
BsiEI CGRYCG 3 cut(s) 75, 539, 880
BsiHKAI GWGCWC 2 cut(s) 318, 625
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 4 cut(s) 519, 535, 539, 880
BslFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BslI CCNNNNNNNGG 7 cut(s) 126, 259, 260, 499, 747, 750, 893
BsmAI GTCTC 4 cut(s) 155, 205, 313, 644
BsmBI CGTCTC 2 cut(s) 205, 313
BsmFI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
BsnI GGCC 3 cut(s) 506, 538, 734
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 318, 625
Bsp143I GATC 5 cut(s) 72, 436, 514, 796, 801
BspACI CCGC 6 cut(s) 225, 289, 482, 605, 741, 834
BspANI GGCC 3 cut(s) 506, 538, 734
BspFNI CGCG 1 cut(s) 836
BspLI GGNNCC 8 cut(s) 131, 265, 533, 554, 555, 666, 692, 754
BspPI GGATC 2 cut(s) 509, 796
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 2 cut(s) 538, 879
BsrI ACTGG 2 cut(s) 462, 662
BssAI RCCGGY 2 cut(s) 538, 879
BssECI CCNNGG 7 cut(s) 48, 192, 204, 518, 728, 744, 892
BssMI GATC 5 cut(s) 72, 436, 514, 796, 801
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 3 cut(s) 127, 194, 894
Bst4CI ACNGT 5 cut(s) 143, 307, 469, 782, 903
Bst6I CTCTTC 1 cut(s) 632
BstACI GRCGYC 1 cut(s) 297
BstBAI YACGTR 1 cut(s) 489
BstC8I GCNNGC 3 cut(s) 159, 540, 544
BstDEI CTNAG 1 cut(s) 58
BstDSI CCRYGG 3 cut(s) 204, 728, 744
BstF5I GGATG 1 cut(s) 405
BstFNI CGCG 1 cut(s) 836
BstHHI GCGC 2 cut(s) 833, 838
BstKTI GATC 5 cut(s) 75, 439, 517, 799, 804
BstMAI GTCTC 4 cut(s) 155, 205, 313, 644
BstMBI GATC 5 cut(s) 72, 436, 514, 796, 801
BstMCI CGRYCG 3 cut(s) 75, 539, 880
BstMWI GCNNNNNNNGC 1 cut(s) 740
BstNI CCWGG 3 cut(s) 127, 194, 894
BstNSI RCATGY 1 cut(s) 856
BstSCI CCNGG 5 cut(s) 125, 192, 517, 518, 892
BstUI CGCG 1 cut(s) 836
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 713
BstZI CGGCCG 1 cut(s) 536
BsuRI GGCC 3 cut(s) 506, 538, 734
BtgI CCRYGG 3 cut(s) 204, 728, 744
BtsCI GGATG 1 cut(s) 405
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 610
Cac8I GCNNGC 3 cut(s) 159, 540, 544
CfoI GCGC 2 cut(s) 833, 838
Cfr10I RCCGGY 2 cut(s) 538, 879
Cfr13I GGNCC 9 cut(s) 4, 129, 532, 553, 607, 690, 732, 753, 782
Cfr9I CCCGGG 1 cut(s) 518
CseI GACGC 1 cut(s) 305
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 3 cut(s) 232, 465, 490
CviAII CATG 5 cut(s) 229, 256, 499, 707, 853
CviQI GTAC 3 cut(s) 232, 465, 490
DdeI CTNAG 1 cut(s) 58
DpnI GATC 5 cut(s) 74, 438, 516, 798, 803
DpnII GATC 5 cut(s) 72, 436, 514, 796, 801
DraIII CACNNNGTG 1 cut(s) 817
EaeI YGGCCR 2 cut(s) 504, 536
EagI CGGCCG 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 632
EarI CTCTTC 1 cut(s) 632
Ecl136II GAGCTC 1 cut(s) 623
EclXI CGGCCG 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 625
Eco47I GGWCC 8 cut(s) 4, 129, 532, 553, 607, 690, 753, 782
Eco52I CGGCCG 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 623
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 518
EcoICRI GAGCTC 1 cut(s) 623
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 3 cut(s) 125, 192, 892
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 625
ErhI CCWWGG 1 cut(s) 48
Esp3I CGTCTC 2 cut(s) 205, 313
FaeI CATG 5 cut(s) 232, 259, 502, 710, 856
FaiI YATR 9 cut(s) 230, 257, 479, 500, 708, 788, 825, 827, 854
FalI AAGNNNNNCTT 2 cut(s) 329, 361
FaqI GGGAC 6 cut(s) 115, 196, 236, 566, 595, 766
FatI CATG 5 cut(s) 228, 255, 498, 706, 852
FauI CCCGC 2 cut(s) 232, 296
FauNDI CATATG 1 cut(s) 825
FblI GTMKAC 2 cut(s) 78, 351
Fnu4HI GCNGC 3 cut(s) 69, 741, 834
FokI GGATG 1 cut(s) 392
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 3 cut(s) 69, 741, 834
FspBI CTAG 1 cut(s) 18
GlaI GCGC 2 cut(s) 832, 837
GluI GCNGC 3 cut(s) 69, 741, 834
GsuI CTGGAG 2 cut(s) 137, 445
HaeIII GGCC 3 cut(s) 506, 538, 734
HapII CCGG 4 cut(s) 519, 535, 539, 880
HgaI GACGC 1 cut(s) 305
HhaI GCGC 2 cut(s) 833, 838
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 232, 259, 502, 710, 856
Hin6I GCGC 2 cut(s) 831, 836
HinP1I GCGC 2 cut(s) 831, 836
HincII GTYRAC 3 cut(s) 352, 472, 862
HindII GTYRAC 3 cut(s) 352, 472, 862
HinfI GANTC 3 cut(s) 332, 637, 820
HpaII CCGG 4 cut(s) 519, 535, 539, 880
Hpy166II GTNNAC 6 cut(s) 79, 352, 472, 610, 690, 862
Hpy188I TCNGA 3 cut(s) 597, 801, 888
Hpy188III TCNNGA 3 cut(s) 184, 261, 805
Hpy8I GTNNAC 6 cut(s) 79, 352, 472, 610, 690, 862
Hpy99I CGWCG 4 cut(s) 147, 299, 353, 782
HpyAV CCTTC 1 cut(s) 776
HpyCH4III ACNGT 5 cut(s) 143, 307, 469, 782, 903
HpyCH4IV ACGT 3 cut(s) 488, 587, 633
HpyCH4V TGCA 2 cut(s) 26, 710
HpyF10VI GCNNNNNNNGC 1 cut(s) 740
HpyF3I CTNAG 1 cut(s) 58
HpySE526I ACGT 3 cut(s) 488, 587, 633
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 232, 259, 502, 710, 856
HspAI GCGC 2 cut(s) 831, 836
KflI GGGWCCC 1 cut(s) 553
KroI GCCGGC 1 cut(s) 538
KroNI GCCGGC 1 cut(s) 540
Kzo9I GATC 5 cut(s) 72, 436, 514, 796, 801
LmnI GCTCC 3 cut(s) 263, 321, 598
Lsp1109I GCAGC 1 cut(s) 80
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 18
MaeII ACGT 3 cut(s) 488, 587, 633
MaeIII GTNAC 1 cut(s) 817
MalI GATC 5 cut(s) 74, 438, 516, 798, 803
MboI GATC 5 cut(s) 72, 436, 514, 796, 801
MboII GAAGA 2 cut(s) 337, 619
MhlI GDGCHC 2 cut(s) 318, 625
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 1 cut(s) 673
MluNI TGGCCA 1 cut(s) 506
MlyI GAGTC 2 cut(s) 646, 814
MmeI TCCRAC 2 cut(s) 346, 885
Mox20I TGGCCA 1 cut(s) 506
MroNI GCCGGC 1 cut(s) 538
MscI TGGCCA 1 cut(s) 506
MslI CAYNNNNRTG 1 cut(s) 711
Msp20I TGGCCA 1 cut(s) 506
MspA1I CMGCKG 2 cut(s) 10, 157
MspI CCGG 4 cut(s) 519, 535, 539, 880
MspR9I CCNGG 5 cut(s) 127, 194, 519, 520, 894
MteI GCGCNGCGC 1 cut(s) 834
MvaI CCWGG 3 cut(s) 127, 194, 894
MvnI CGCG 1 cut(s) 836
MwoI GCNNNNNNNGC 1 cut(s) 740
NaeI GCCGGC 1 cut(s) 540
NciI CCSGG 2 cut(s) 519, 520
NdeI CATATG 1 cut(s) 825
NdeII GATC 5 cut(s) 72, 436, 514, 796, 801
NgoMIV GCCGGC 1 cut(s) 538
NlaIII CATG 5 cut(s) 232, 259, 502, 710, 856
NlaIV GGNNCC 8 cut(s) 131, 265, 533, 554, 555, 666, 692, 754
NmuCI GTSAC 1 cut(s) 817
NspI RCATGY 1 cut(s) 856
PcsI WCGNNNNNNNCGW 5 cut(s) 81, 303, 348, 593, 639
PdiI GCCGGC 1 cut(s) 540
PfeI GAWTC 1 cut(s) 332
PflFI GACNNNGTC 1 cut(s) 586
PflMI CCANNNNNTGG 1 cut(s) 499
PkrI GCNGC 3 cut(s) 70, 742, 835
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 2 cut(s) 645, 814
PpsI GAGTC 2 cut(s) 645, 814
Ppu21I YACGTR 1 cut(s) 489
PpuMI RGGWCCY 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 553
Psp6I CCWGG 3 cut(s) 125, 192, 892
PspGI CCWGG 3 cut(s) 125, 192, 892
PspN4I GGNNCC 8 cut(s) 131, 265, 533, 554, 555, 666, 692, 754
PspPI GGNCC 9 cut(s) 4, 129, 532, 553, 607, 690, 732, 753, 782
PspPPI RGGWCCY 1 cut(s) 553
PsyI GACNNNGTC 1 cut(s) 586
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 10, 157
RsaI GTAC 3 cut(s) 233, 466, 491
RsaNI GTAC 3 cut(s) 232, 465, 490
RseI CAYNNNNRTG 1 cut(s) 711
SacI GAGCTC 1 cut(s) 625
SalI GTCGAC 1 cut(s) 350
SatI GCNGC 3 cut(s) 69, 741, 834
Sau3AI GATC 5 cut(s) 72, 436, 514, 796, 801
Sau96I GGNCC 9 cut(s) 4, 129, 532, 553, 607, 690, 732, 753, 782
SchI GAGTC 2 cut(s) 646, 814
ScrFI CCNGG 5 cut(s) 127, 194, 519, 520, 894
SduI GDGCHC 2 cut(s) 318, 625
SexAI ACCWGGT 1 cut(s) 125
SinI GGWCC 8 cut(s) 4, 129, 532, 553, 607, 690, 753, 782
SmaI CCCGGG 1 cut(s) 520
SmiMI CAYNNNNRTG 1 cut(s) 711
Sse9I AATT 1 cut(s) 673
SsiI CCGC 6 cut(s) 225, 289, 482, 605, 741, 834
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 1 cut(s) 625
StyD4I CCNGG 5 cut(s) 125, 192, 517, 518, 892
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 5 cut(s) 143, 307, 469, 782, 903
TaiI ACGT 3 cut(s) 491, 590, 636
TaqI TCGA 5 cut(s) 145, 294, 351, 654, 777
TaqII GACCGA 1 cut(s) 78
TasI AATT 1 cut(s) 673
TauI GCSGC 2 cut(s) 743, 836
TfiI GAWTC 1 cut(s) 332
TscAI CASTG 1 cut(s) 617
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 1 cut(s) 466
TspGWI ACGGA 2 cut(s) 193, 301
TspMI CCCGGG 1 cut(s) 518
TspRI CASTG 1 cut(s) 617
Tth111I GACNNNGTC 1 cut(s) 586
Van91I CCANNNNNTGG 1 cut(s) 499
VpaK11BI GGWCC 8 cut(s) 4, 129, 532, 553, 607, 690, 753, 782
XceI RCATGY 1 cut(s) 856
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XmaI CCCGGG 1 cut(s) 518
XmiI GTMKAC 2 cut(s) 78, 351
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.