Rh3AG282800

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000677
Physical Location & Seq
Reverse (-)
33028489 .. 33030506
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG282800.1

Sequence Viewer

Length: 603 bp
ATGACCCGGGTGGACGTGGTGGATCCGGATACGGGGTTGCCGGTTAAACGCGACGGTTCGTCGCTTGGGGAGGTGGTTTTCAGAGGCGGGTGTGTGATGTTGGGTTATCTGAAAGACCCAAAAGGCACGGCCAGTTGCATGAAAGACGGTTGGTTTTACACCGGCGACGTGGCGGTGATGCACCCGGACGGGTATCTGGAGATCAAGGACAGATCCAAGGACGTGATCATAAGCGGGGGAGAGAACTTGAGCAGCGTGGAGGTGGAGTCGGTACTCTACACCAACTCCGCGGTGAACGAGGCGGCCGTGGTGGCGAGGCCTGACGAGTTCTGGGGGGAGACACCGTGCGCGTTTGTGAGCCTGAAAGATGACGTGGGGCGGAGGCCGACGGAGAAGGAGATGATAGAGTATTGCAGAGGAAAGCTGCCGAATTTCATGGTGCCGAAGACGGTGGTGTTTATTGAGGAGCTGCCGAAGACGTCGACGGGGAAAATTCAGAAGTTCGTGCTGAGAGAGATGGCCAAGGCCTTGGGGTCCACAAGGCTGAGTCGGATGTCAATTCCAAATTGGTGGAATCTGATGATCAATACCTCTGTGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

200

Amino Acids

22.14

Weight (kDa)

5.59

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 87 - 164 4.9e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 482
AccII CGCG 3 cut(s) 51, 290, 350
AccIII TCCGGA 1 cut(s) 25
AciI CCGC 7 cut(s) 87, 173, 234, 288, 290, 302, 379
AclWI GGATC 3 cut(s) 17, 30, 207
AcoI YGGCCR 3 cut(s) 129, 303, 519
AcsI RAATTY 2 cut(s) 430, 492
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 1 cut(s) 273
AfiI CCNNNNNNNGG 1 cut(s) 32
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 4 cut(s) 16, 169, 223, 373
AluBI AGCT 2 cut(s) 424, 469
AluI AGCT 2 cut(s) 424, 469
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 3 cut(s) 17, 30, 207
Ama87I CYCGRG 1 cut(s) 6
Aor13HI TCCGGA 1 cut(s) 25
AoxI GGCC 6 cut(s) 129, 303, 317, 383, 519, 525
ApeKI GCWGC 3 cut(s) 252, 424, 469
ApoI RAATTY 2 cut(s) 430, 492
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 534
AsuC2I CCSGG 3 cut(s) 7, 8, 185
AsuHPI GGTGA 2 cut(s) 187, 304
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 534
BalI TGGCCA 1 cut(s) 521
BamHI GGATCC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 452, 482
BbvI GCAGC 3 cut(s) 264, 411, 456
BccI CCATC 1 cut(s) 511
BceAI ACGGC 2 cut(s) 144, 290
BciVI GTATCC 1 cut(s) 22
BclI TGATCA 2 cut(s) 225, 582
BcnI CCSGG 3 cut(s) 7, 8, 185
BcoDI GTCTC 1 cut(s) 332
BfuI GTATCC 1 cut(s) 22
BglI GCCNNNNNGGC 1 cut(s) 311
BisI GCNGC 4 cut(s) 253, 303, 425, 470
BlsI GCNGC 4 cut(s) 254, 304, 426, 471
Bme1390I CCNGG 3 cut(s) 7, 8, 185
Bme18I GGWCC 1 cut(s) 534
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 6
BmgBI CACGTC 4 cut(s) 16, 169, 223, 373
BmgT120I GGNCC 1 cut(s) 534
BmiI GGNNCC 3 cut(s) 24, 441, 535
BmrFI CCNGG 3 cut(s) 7, 8, 185
BmsI GCATC 1 cut(s) 168
BpiI GAAGAC 2 cut(s) 452, 482
BpmI CTGGAG 1 cut(s) 218
BpuEI CTTGAG 1 cut(s) 268
BpuMI CCSGG 3 cut(s) 7, 8, 185
BsaHI GRCGYC 1 cut(s) 479
BsaJI CCNNGG 6 cut(s) 6, 216, 288, 306, 522, 528
BsaWI WCCGGW 1 cut(s) 25
BsaXI ACNNNNNCTCC 6 cut(s) 251, 269, 281, 299, 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 32
Bse118I RCCGGY 2 cut(s) 40, 161
Bse1I ACTGG 1 cut(s) 132
BseAI TCCGGA 1 cut(s) 25
BseDI CCNNGG 6 cut(s) 6, 216, 288, 306, 522, 528
BseGI GGATG 1 cut(s) 558
BseLI CCNNNNNNNGG 1 cut(s) 32
BseMII CTCAG 2 cut(s) 500, 536
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 479
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 3 cut(s) 264, 411, 456
Bsh1236I CGCG 3 cut(s) 51, 290, 350
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 6 cut(s) 131, 305, 319, 385, 521, 527
BshNI GGYRCC 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 5 cut(s) 7, 26, 41, 162, 185
BslI CCNNNNNNNGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 332
BsnI GGCC 6 cut(s) 131, 305, 319, 385, 521, 527
BsoBI CYCGRG 1 cut(s) 6
Bsp13I TCCGGA 1 cut(s) 25
Bsp143I GATC 5 cut(s) 22, 201, 212, 225, 582
BspACI CCGC 7 cut(s) 87, 173, 234, 288, 290, 302, 379
BspANI GGCC 6 cut(s) 131, 305, 319, 385, 521, 527
BspCNI CTCAG 2 cut(s) 501, 537
BspEI TCCGGA 1 cut(s) 25
BspFNI CGCG 3 cut(s) 51, 290, 350
BspLI GGNNCC 3 cut(s) 24, 441, 535
BspPI GGATC 3 cut(s) 17, 30, 207
BspT107I GGYRCC 1 cut(s) 439
BsrFI RCCGGY 2 cut(s) 40, 161
BsrI ACTGG 1 cut(s) 132
BssAI RCCGGY 2 cut(s) 40, 161
BssECI CCNNGG 6 cut(s) 6, 216, 288, 306, 522, 528
BssMI GATC 5 cut(s) 22, 201, 212, 225, 582
BssNI GRCGYC 1 cut(s) 479
BssT1I CCWWGG 3 cut(s) 216, 522, 528
Bst4CI ACNGT 4 cut(s) 56, 149, 345, 451
BstACI GRCGYC 1 cut(s) 479
BstDEI CTNAG 3 cut(s) 509, 545, 600
BstDSI CCRYGG 2 cut(s) 288, 306
BstF5I GGATG 1 cut(s) 558
BstFNI CGCG 3 cut(s) 51, 290, 350
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 5 cut(s) 25, 204, 215, 228, 585
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 5 cut(s) 22, 201, 212, 225, 582
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstSCI CCNGG 3 cut(s) 5, 6, 183
BstUI CGCG 3 cut(s) 51, 290, 350
BstV1I GCAGC 3 cut(s) 264, 411, 456
BstV2I GAAGAC 2 cut(s) 452, 482
BstX2I RGATCY 2 cut(s) 22, 212
BstXI CCANNNNNNTGG 2 cut(s) 529, 570
BstYI RGATCY 2 cut(s) 22, 212
BstZI CGGCCG 1 cut(s) 303
BsuI GTATCC 1 cut(s) 22
BsuRI GGCC 6 cut(s) 131, 305, 319, 385, 521, 527
BtgI CCRYGG 2 cut(s) 288, 306
BtrI CACGTC 4 cut(s) 16, 169, 223, 373
BtsCI GGATG 1 cut(s) 558
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 2 cut(s) 40, 161
Cfr13I GGNCC 1 cut(s) 534
Cfr42I CCGCGG 1 cut(s) 291
Cfr9I CCCGGG 1 cut(s) 6
Csp6I GTAC 1 cut(s) 272
CviAII CATG 2 cut(s) 139, 436
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 3 cut(s) 509, 545, 600
DpnI GATC 5 cut(s) 24, 203, 214, 227, 584
DpnII GATC 5 cut(s) 22, 201, 212, 225, 582
DriI GACNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 3 cut(s) 129, 303, 519
EagI CGGCCG 1 cut(s) 303
Eam1105I GACNNNNNGTC 1 cut(s) 58
EciI GGCGGA 1 cut(s) 394
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 3 cut(s) 216, 522, 528
Eco147I AGGCCT 2 cut(s) 319, 527
Eco47I GGWCC 1 cut(s) 534
Eco52I CGGCCG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 216, 522, 528
ErhI CCWWGG 3 cut(s) 216, 522, 528
FaeI CATG 2 cut(s) 142, 439
FaiI YATR 3 cut(s) 140, 230, 437
FatI CATG 2 cut(s) 138, 435
FauI CCCGC 2 cut(s) 80, 227
FbaI TGATCA 2 cut(s) 225, 582
FblI GTMKAC 1 cut(s) 482
Fnu4HI GCNGC 4 cut(s) 253, 303, 425, 470
FokI GGATG 1 cut(s) 565
Fsp4HI GCNGC 4 cut(s) 253, 303, 425, 470
GlaI GCGC 1 cut(s) 349
GluI GCNGC 4 cut(s) 253, 303, 425, 470
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 6 cut(s) 131, 305, 319, 385, 521, 527
HapII CCGG 5 cut(s) 7, 26, 41, 162, 185
HhaI GCGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 2 cut(s) 142, 439
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 1 cut(s) 483
HindII GTYRAC 1 cut(s) 483
HinfI GANTC 3 cut(s) 266, 547, 574
HpaII CCGG 5 cut(s) 7, 26, 41, 162, 185
HphI GGTGA 2 cut(s) 187, 304
Hpy166II GTNNAC 4 cut(s) 13, 295, 483, 537
Hpy188I TCNGA 5 cut(s) 83, 111, 498, 552, 579
Hpy188III TCNNGA 2 cut(s) 26, 197
Hpy8I GTNNAC 4 cut(s) 13, 295, 483, 537
Hpy99I CGWCG 6 cut(s) 56, 64, 170, 391, 484, 487
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 4 cut(s) 56, 149, 345, 451
HpyCH4IV ACGT 5 cut(s) 15, 168, 222, 372, 479
HpyCH4V TGCA 3 cut(s) 138, 181, 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 3 cut(s) 509, 545, 600
HpySE526I ACGT 5 cut(s) 15, 168, 222, 372, 479
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 2 cut(s) 142, 439
HspAI GCGC 1 cut(s) 348
Kpn2I TCCGGA 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 225, 582
KspI CCGCGG 1 cut(s) 291
Kzo9I GATC 5 cut(s) 22, 201, 212, 225, 582
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 3 cut(s) 264, 411, 456
LweI GCATC 1 cut(s) 168
MaeII ACGT 5 cut(s) 15, 168, 222, 372, 479
MalI GATC 5 cut(s) 24, 203, 214, 227, 584
MboI GATC 5 cut(s) 22, 201, 212, 225, 582
MboII GAAGA 2 cut(s) 457, 487
MflI RGATCY 2 cut(s) 22, 212
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 4 cut(s) 430, 492, 558, 565
MluNI TGGCCA 1 cut(s) 521
MlyI GAGTC 2 cut(s) 275, 556
MmeI TCCRAC 1 cut(s) 530
MnlI CCTC 9 cut(s) 64, 77, 253, 292, 309, 375, 410, 457, 601
Mox20I TGGCCA 1 cut(s) 521
MroI TCCGGA 1 cut(s) 25
MscI TGGCCA 1 cut(s) 521
MseI TTAA 1 cut(s) 45
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 5 cut(s) 7, 26, 41, 162, 185
MspR9I CCNGG 3 cut(s) 7, 8, 185
MvnI CGCG 3 cut(s) 51, 290, 350
MwoI GCNNNNNNNGC 1 cut(s) 311
NciI CCSGG 3 cut(s) 7, 8, 185
NdeII GATC 5 cut(s) 22, 201, 212, 225, 582
NlaIII CATG 2 cut(s) 142, 439
NlaIV GGNNCC 3 cut(s) 24, 441, 535
PceI AGGCCT 2 cut(s) 319, 527
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 1 cut(s) 574
PkrI GCNGC 4 cut(s) 254, 304, 426, 471
PleI GAGTC 2 cut(s) 274, 555
PpsI GAGTC 2 cut(s) 274, 555
PspN4I GGNNCC 3 cut(s) 24, 441, 535
PspPI GGNCC 1 cut(s) 534
PsuI RGATCY 2 cut(s) 22, 212
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
SacII CCGCGG 1 cut(s) 291
SalI GTCGAC 1 cut(s) 481
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 4 cut(s) 253, 303, 425, 470
Sau3AI GATC 5 cut(s) 22, 201, 212, 225, 582
Sau96I GGNCC 1 cut(s) 534
SchI GAGTC 2 cut(s) 275, 556
ScrFI CCNGG 3 cut(s) 7, 8, 185
SfaNI GCATC 1 cut(s) 168
Sfr303I CCGCGG 1 cut(s) 291
SgrAI CRCCGGYG 1 cut(s) 161
SgrBI CCGCGG 1 cut(s) 291
SgrDI CGTCGACG 1 cut(s) 481
SinI GGWCC 1 cut(s) 534
SmaI CCCGGG 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
Sse9I AATT 4 cut(s) 430, 492, 558, 565
SseBI AGGCCT 2 cut(s) 319, 527
SsiI CCGC 7 cut(s) 87, 173, 234, 288, 290, 302, 379
StuI AGGCCT 2 cut(s) 319, 527
StyD4I CCNGG 3 cut(s) 5, 6, 183
StyI CCWWGG 3 cut(s) 216, 522, 528
TaaI ACNGT 4 cut(s) 56, 149, 345, 451
TaiI ACGT 5 cut(s) 18, 171, 225, 375, 482
TaqI TCGA 1 cut(s) 482
TasI AATT 4 cut(s) 430, 492, 558, 565
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 1 cut(s) 574
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseI GCWGC 3 cut(s) 252, 424, 469
TspDTI ATGAA 2 cut(s) 155, 424
TspGWI ACGGA 1 cut(s) 404
TspMI CCCGGG 1 cut(s) 6
VpaK11BI GGWCC 1 cut(s) 534
XapI RAATTY 2 cut(s) 430, 492
XmaI CCCGGG 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 482
ZraI GACGTC 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.