Prupe.3G011200_v2.0.a1

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000677
Physical Location & Seq
Reverse (-)
702183 .. 703868
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G011200.1

Sequence Viewer

Length: 1686 bp
ATGGACCAGTTGAAGCCAAGCCCTGCAAATCTCTGCCCTCTCACTCCCCTCACTCTCCTCGAAAGAGCTGCGGTTGTCAACGGCGACTGCCCATCCATCATCTACACCGCCACCACTACTACCACAACCTACACTTGGTCCCAAACTCACCGTCGATGCCTCCAGCTGGCCTCCTCCATCTCCTCCCTCGTCAAGCGCTCCCAGGTGGTCTCCGTGGTGGCCCCCAACACCCCAGCCATGTACGAGCTTCACTTTGCGGTCCCCATGTCCGGGGCCATCCTCAACAACATCAACACCCGCCTCGACGCCCACACCATCTCCATCATCCTGCGCCACAGCGAATCAAAGCTCATCTTCGTCGACCACCTCTCCGTCTCTCTCACCCTCCAAGCACTCTCTCTCTTCCCGCCTCACCTCCCAAAACCGCTTCTCATCCTCATCACCGATGATGAGCACCGCTCAACACCAATCCCAGCCGTTGATAATAAGTACTTCCTTGACACTTACGAGAATCTAGTGAGGAAAGGGAATCCGTCGTTCGAGTGGGTCCGGCCGGCTAGCGAGTGGGCCCCGATGGTTTTAAACTACACCTCCGGAACGACGTCGTCTCCGAAAGGCGTCGTGCACAGCCACAGAGGCTTGTACATCGTCACCTTGGTTTCCATCATAGACTGGTCCGTACCCAAACACTGCGTGTACCTGTGGACTTTACCGATGTTCCACTCAAACGGGTGGGGGTACCCGTGGGCCATGGCCGCAGTGGGTGGAACCAACATTTGCCTCCGCAAGTTCGACGCTCCCAAAATCTACAGCTCAATCCGAGCCCACAACGTCACTCACATGTGCGCCGCGCCGGTCGTGCTCAACATGCTCGTCAACCACCCGATATCCGTCCCGCTCCCTCACCCGGTTCACATCCTCACCGGTGGAGCCCCGCCGCCGTCAAGCGTCCTGCTTCGGGCCGAGTCAATCGGGTTCTTGGTTTGCCACGGTTACGGGTTGACGGAAACGGGTGGGGTGGTCGTGTCATGTGCTTGGAAGCCGGAGTGGAATAAATACCCGATGACGGAAAAAGCGAAGCTGAAAGCGAGACAAGGGGTGAGGACGATTGGGATGACGGAGGTGGACGTGGTGGATCCGGATTCGGGTTCGCCAGTGAAGCGAGACGGGTCGGAGATTGGGGAGATAGTGTTCAAAGGCGGGTGCGTGATGCTGGGGTATTTCAAAGACCCGGAGAGTACAGCAAAATGTTTGAAGAAAAACGGGTGGTTTTATACGGGTGACGTGGGCGTGATGCATCCCGATGGCTATTTGGAAATTAAGGACAGATCCAAGGACGTTATTATAACAGGGGGCGAGAATCTGAGCAGCGTGGAGGTTGAGTCCGTGCTCTATGCGAATCCGGCTGTGAACGAAGCGGCGGTGGTGGCGAAGCCCGACGAGTTCTGGGGGGAGACGCCGTGCGCGTTTGTGAGCTTGAAGAGTGACGTGAGCCCTAAGCCGACGGAGAAGGAGATCATGGAGTATTGCAGGGAAAATCTGCCGCACTACATGGTGCCCAAAACGGTGGTGTTTAAGGACGAGCTCCCGAAGACTTCGACTGGGAAGATTCAGAAGTTTGTGCTCAGAGAGATTGCCAAGGCGATTCGGTCGTCGTCGTCGTTGAGGACGACAAGTCGTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

562

Amino Acids

61.79

Weight (kDa)

8.46

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1346
AatII GACGTC 1 cut(s) 605
Acc65I GGTACC 1 cut(s) 738
AccB1I GGYRCC 2 cut(s) 738, 1555
AccBSI CCGCTC 2 cut(s) 459, 898
AccI GTMKAC 1 cut(s) 360
AccII CGCG 2 cut(s) 851, 1466
AccIII TCCGGA 2 cut(s) 593, 1138
AclWI GGATC 3 cut(s) 1130, 1143, 1323
AcoI YGGCCR 2 cut(s) 551, 753
AcyI GRCGYC 4 cut(s) 306, 602, 618, 1457
AdeI CACNNNGTG 1 cut(s) 694
AfaI GTAC 7 cut(s) 242, 491, 644, 681, 698, 740, 1240
AfeI AGCGCT 1 cut(s) 197
AfiI CCNNNNNNNGG 8 cut(s) 135, 166, 269, 270, 907, 958, 1066, 1145
AflIII ACRYGT 1 cut(s) 840
AgeI ACCGGT 1 cut(s) 923
AgsI TTSAA 5 cut(s) 13, 1195, 1225, 1255, 1480
AhdI GACNNNNNGTC 1 cut(s) 1674
AjiI CACGTC 3 cut(s) 1129, 1285, 1489
AjnI CCWGG 1 cut(s) 201
AloI GAACNNNNNNTCC 2 cut(s) 1175, 1207
AluBI AGCT 8 cut(s) 68, 166, 247, 349, 813, 1081, 1476, 1585
AluI AGCT 8 cut(s) 68, 166, 247, 349, 813, 1081, 1476, 1585
Alw21I GWGCWC 6 cut(s) 456, 627, 864, 1392, 1587, 1626
Alw26I GTCTC 6 cut(s) 214, 379, 612, 1084, 1158, 1448
Alw44I GTGCAC 1 cut(s) 623
AlwI GGATC 3 cut(s) 1130, 1143, 1323
Aor13HI TCCGGA 2 cut(s) 593, 1138
Aor51HI AGCGCT 1 cut(s) 197
AoxI GGCC 8 cut(s) 168, 219, 273, 551, 567, 747, 753, 960
ApaI GGGCCC 1 cut(s) 571
ApaLI GTGCAC 1 cut(s) 623
ApeKI GCWGC 2 cut(s) 68, 1368
ArsI GACNNNNNNTTYG 2 cut(s) 136, 168
AsiGI ACCGGT 1 cut(s) 923
Asp718I GGTACC 1 cut(s) 738
AspLEI GCGC 5 cut(s) 198, 333, 848, 853, 1466
AsuC2I CCSGG 3 cut(s) 271, 908, 1232
AsuHPI GGTGA 9 cut(s) 140, 373, 404, 433, 643, 896, 913, 1111, 1292
AsuNHI GCTAGC 1 cut(s) 557
AvaII GGWCC 5 cut(s) 4, 138, 259, 547, 675
BaeGI GKGCMC 3 cut(s) 571, 627, 1560
BamHI GGATCC 1 cut(s) 1135
BanI GGYRCC 2 cut(s) 738, 1555
BanII GRGCYC 5 cut(s) 571, 826, 934, 1496, 1587
BbsI GAAGAC 1 cut(s) 1598
Bbv12I GWGCWC 6 cut(s) 456, 627, 864, 1392, 1587, 1626
BbvI GCAGC 2 cut(s) 55, 1380
BccI CCATC 9 cut(s) 100, 104, 185, 284, 323, 329, 568, 671, 1298
BceAI ACGGC 4 cut(s) 97, 461, 925, 1444
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 1 cut(s) 203
BcnI CCSGG 3 cut(s) 271, 908, 1232
BcoDI GTCTC 6 cut(s) 214, 379, 612, 1084, 1158, 1448
BfaI CTAG 2 cut(s) 515, 558
BfmI CTRYAG 1 cut(s) 808
BfoI RGCGCY 1 cut(s) 199
BglI GCCNNNNNGGC 1 cut(s) 636
BisI GCNGC 7 cut(s) 69, 756, 849, 938, 1369, 1419, 1544
BlsI GCNGC 7 cut(s) 70, 757, 850, 939, 1370, 1420, 1545
BmcAI AGTACT 1 cut(s) 491
Bme1390I CCNGG 4 cut(s) 203, 271, 908, 1232
Bme18I GGWCC 5 cut(s) 4, 138, 259, 547, 675
BmeRI GACNNNNNGTC 1 cut(s) 1674
BmgBI CACGTC 3 cut(s) 1129, 1285, 1489
BmrFI CCNGG 4 cut(s) 203, 271, 908, 1232
BmrI ACTGGG 1 cut(s) 1611
BmsI GCATC 4 cut(s) 146, 1200, 1284, 1306
BmtI GCTAGC 1 cut(s) 561
BmuI ACTGGG 1 cut(s) 1611
BpiI GAAGAC 1 cut(s) 1598
BplI GAGNNNNNCTC 2 cut(s) 443, 475
BpmI CTGGAG 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 1497
BpuMI CCSGG 3 cut(s) 271, 908, 1232
BsaHI GRCGYC 4 cut(s) 306, 602, 618, 1457
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 9 cut(s) 201, 213, 270, 654, 743, 750, 988, 1332, 1638
BsaWI WCCGGW 3 cut(s) 593, 923, 1138
Bsc4I CCNNNNNNNGG 8 cut(s) 135, 166, 269, 270, 907, 958, 1066, 1145
Bse118I RCCGGY 3 cut(s) 553, 853, 923
Bse1I ACTGG 4 cut(s) 7, 677, 1154, 1606
BseAI TCCGGA 2 cut(s) 593, 1138
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 9 cut(s) 201, 213, 270, 654, 743, 750, 988, 1332, 1638
BseGI GGATG 7 cut(s) 92, 276, 324, 432, 915, 1119, 1297
BseLI CCNNNNNNNGG 8 cut(s) 135, 166, 269, 270, 907, 958, 1066, 1145
BseMII CTCAG 2 cut(s) 1355, 1639
BseNI ACTGG 4 cut(s) 7, 677, 1154, 1606
BseRI GAGGAG 3 cut(s) 47, 163, 172
BseSI GKGCMC 3 cut(s) 571, 627, 1560
BseX3I CGGCCG 1 cut(s) 551
BseXI GCAGC 2 cut(s) 55, 1380
BseYI CCCAGC 3 cut(s) 232, 472, 1213
Bsh1236I CGCG 2 cut(s) 851, 1466
Bsh1285I CGRYCG 3 cut(s) 554, 858, 1652
BshFI GGCC 8 cut(s) 170, 221, 275, 553, 569, 749, 755, 962
BshNI GGYRCC 2 cut(s) 738, 1555
BshTI ACCGGT 1 cut(s) 923
BsiEI CGRYCG 3 cut(s) 554, 858, 1652
BsiHKAI GWGCWC 6 cut(s) 456, 627, 864, 1392, 1587, 1626
BslFI GGGAC 3 cut(s) 124, 245, 878
BslI CCNNNNNNNGG 8 cut(s) 135, 166, 269, 270, 907, 958, 1066, 1145
BsmAI GTCTC 6 cut(s) 214, 379, 612, 1084, 1158, 1448
BsmBI CGTCTC 4 cut(s) 379, 612, 1158, 1448
BsmFI GGGAC 3 cut(s) 124, 245, 878
BsnI GGCC 8 cut(s) 170, 221, 275, 553, 569, 749, 755, 962
Bso31I GGTCTC 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 567
Bsp13I TCCGGA 2 cut(s) 593, 1138
Bsp1407I TGTACA 1 cut(s) 642
Bsp143I GATC 3 cut(s) 1135, 1328, 1515
Bsp19I CCATGG 1 cut(s) 750
BspANI GGCC 8 cut(s) 170, 221, 275, 553, 569, 749, 755, 962
BspCNI CTCAG 2 cut(s) 1356, 1638
BspEI TCCGGA 2 cut(s) 593, 1138
BspFNI CGCG 2 cut(s) 851, 1466
BspOI GCTAGC 1 cut(s) 561
BspPI GGATC 3 cut(s) 1130, 1143, 1323
BspT107I GGYRCC 2 cut(s) 738, 1555
BspTNI GGTCTC 1 cut(s) 214
BsrBI CCGCTC 2 cut(s) 459, 898
BsrFI RCCGGY 3 cut(s) 553, 853, 923
BsrGI TGTACA 1 cut(s) 642
BsrI ACTGG 4 cut(s) 7, 677, 1154, 1606
BssAI RCCGGY 3 cut(s) 553, 853, 923
BssECI CCNNGG 9 cut(s) 201, 213, 270, 654, 743, 750, 988, 1332, 1638
BssMI GATC 3 cut(s) 1135, 1328, 1515
BssNI GRCGYC 4 cut(s) 306, 602, 618, 1457
BssT1I CCWWGG 4 cut(s) 654, 750, 1332, 1638
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 152, 992, 1567
Bst6I CTCTTC 2 cut(s) 407, 1475
BstACI GRCGYC 4 cut(s) 306, 602, 618, 1457
BstAUI TGTACA 1 cut(s) 642
BstC8I GCNNGC 3 cut(s) 168, 555, 559
BstDEI CTNAG 3 cut(s) 1364, 1497, 1625
BstDSI CCRYGG 4 cut(s) 213, 743, 750, 988
BstF5I GGATG 7 cut(s) 92, 276, 324, 432, 915, 1119, 1297
BstFNI CGCG 2 cut(s) 851, 1466
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 5 cut(s) 198, 333, 848, 853, 1466
BstKTI GATC 3 cut(s) 1138, 1331, 1518
BstMAI GTCTC 6 cut(s) 214, 379, 612, 1084, 1158, 1448
BstMBI GATC 3 cut(s) 1135, 1328, 1515
BstMCI CGRYCG 3 cut(s) 554, 858, 1652
BstMWI GCNNNNNNNGC 7 cut(s) 636, 755, 859, 868, 1159, 1403, 1427
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 844, 871
BstSCI CCNGG 4 cut(s) 201, 269, 906, 1230
BstSFI CTRYAG 1 cut(s) 808
BstSLI GKGCMC 3 cut(s) 571, 627, 1560
BstUI CGCG 2 cut(s) 851, 1466
BstV1I GCAGC 2 cut(s) 55, 1380
BstV2I GAAGAC 1 cut(s) 1598
BstX2I RGATCY 2 cut(s) 1135, 1328
BstXI CCANNNNNNTGG 1 cut(s) 1567
BstYI RGATCY 2 cut(s) 1135, 1328
BstZI CGGCCG 1 cut(s) 551
BsuRI GGCC 8 cut(s) 170, 221, 275, 553, 569, 749, 755, 962
BtgI CCRYGG 4 cut(s) 213, 743, 750, 988
BtrI CACGTC 3 cut(s) 1129, 1285, 1489
BtsCI GGATG 7 cut(s) 92, 276, 324, 432, 915, 1119, 1297
BtsI GCAGTG 2 cut(s) 688, 765
BtsIMutI CAGTG 3 cut(s) 688, 765, 1161
Cac8I GCNNGC 3 cut(s) 168, 555, 559
CfoI GCGC 5 cut(s) 198, 333, 848, 853, 1466
Cfr10I RCCGGY 3 cut(s) 553, 853, 923
CseI GACGC 5 cut(s) 314, 607, 803, 937, 1465
Csp6I GTAC 7 cut(s) 241, 490, 643, 680, 697, 739, 1239
CspAI ACCGGT 1 cut(s) 923
CviAII CATG 8 cut(s) 238, 265, 751, 841, 868, 1029, 1519, 1552
CviQI GTAC 7 cut(s) 241, 490, 643, 680, 697, 739, 1239
DdeI CTNAG 3 cut(s) 1364, 1497, 1625
DpnI GATC 3 cut(s) 1137, 1330, 1517
DpnII GATC 3 cut(s) 1135, 1328, 1515
DraI TTTAAA 1 cut(s) 582
DraIII CACNNNGTG 1 cut(s) 694
DriI GACNNNNNGTC 1 cut(s) 1674
EaeI YGGCCR 2 cut(s) 551, 753
EagI CGGCCG 1 cut(s) 551
Eam1104I CTCTTC 2 cut(s) 407, 1475
Eam1105I GACNNNNNGTC 1 cut(s) 1674
EarI CTCTTC 2 cut(s) 407, 1475
Ecl136II GAGCTC 1 cut(s) 1585
EclXI CGGCCG 1 cut(s) 551
Eco130I CCWWGG 4 cut(s) 654, 750, 1332, 1638
Eco24I GRGCYC 5 cut(s) 571, 826, 934, 1496, 1587
Eco31I GGTCTC 1 cut(s) 214
Eco32I GATATC 1 cut(s) 888
Eco47I GGWCC 5 cut(s) 4, 138, 259, 547, 675
Eco47III AGCGCT 1 cut(s) 197
Eco52I CGGCCG 1 cut(s) 551
Eco53kI GAGCTC 1 cut(s) 1585
EcoICRI GAGCTC 1 cut(s) 1585
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 888
EcoT14I CCWWGG 4 cut(s) 654, 750, 1332, 1638
EcoT22I ATGCAT 1 cut(s) 1299
EcoT38I GRGCYC 5 cut(s) 571, 826, 934, 1496, 1587
ErhI CCWWGG 4 cut(s) 654, 750, 1332, 1638
Esp3I CGTCTC 4 cut(s) 379, 612, 1158, 1448
FaeI CATG 8 cut(s) 241, 268, 754, 844, 871, 1032, 1522, 1555
FalI AAGNNNNNCTT 2 cut(s) 338, 370
FaqI GGGAC 3 cut(s) 124, 245, 878
FatI CATG 8 cut(s) 237, 264, 750, 840, 867, 1028, 1518, 1551
FauI CCCGC 5 cut(s) 305, 414, 903, 942, 1193
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 7 cut(s) 69, 756, 849, 938, 1369, 1419, 1544
FokI GGATG 7 cut(s) 79, 263, 311, 419, 902, 1126, 1284
FriOI GRGCYC 5 cut(s) 571, 826, 934, 1496, 1587
Fsp4HI GCNGC 7 cut(s) 69, 756, 849, 938, 1369, 1419, 1544
FspBI CTAG 2 cut(s) 515, 558
GlaI GCGC 5 cut(s) 197, 332, 847, 852, 1465
GluI GCNGC 7 cut(s) 69, 756, 849, 938, 1369, 1419, 1544
GsaI CCCAGC 3 cut(s) 236, 476, 1217
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 8 cut(s) 170, 221, 275, 553, 569, 749, 755, 962
HgaI GACGC 5 cut(s) 314, 607, 803, 937, 1465
HhaI GCGC 5 cut(s) 198, 333, 848, 853, 1466
Hin1I GRCGYC 4 cut(s) 306, 602, 618, 1457
Hin1II CATG 8 cut(s) 241, 268, 754, 844, 871, 1032, 1522, 1555
Hin6I GCGC 5 cut(s) 196, 331, 846, 851, 1464
HinP1I GCGC 5 cut(s) 196, 331, 846, 851, 1464
HincII GTYRAC 4 cut(s) 79, 361, 877, 1002
HindII GTYRAC 4 cut(s) 79, 361, 877, 1002
HphI GGTGA 9 cut(s) 140, 373, 404, 433, 643, 896, 913, 1111, 1292
Hpy188I TCNGA 6 cut(s) 612, 821, 1174, 1365, 1614, 1628
Hpy188III TCNNGA 4 cut(s) 594, 1139, 1301, 1588
HpyAV CCTTC 1 cut(s) 1504
HpyCH4III ACNGT 3 cut(s) 152, 992, 1567
HpyCH4IV ACGT 6 cut(s) 602, 831, 1128, 1284, 1338, 1488
HpyCH4V TGCA 4 cut(s) 26, 625, 1297, 1530
HpyF10VI GCNNNNNNNGC 7 cut(s) 636, 755, 859, 868, 1159, 1403, 1427
HpyF3I CTNAG 3 cut(s) 1364, 1497, 1625
HpySE526I ACGT 6 cut(s) 602, 831, 1128, 1284, 1338, 1488
Hsp92I GRCGYC 4 cut(s) 306, 602, 618, 1457
Hsp92II CATG 8 cut(s) 241, 268, 754, 844, 871, 1032, 1522, 1555
HspAI GCGC 5 cut(s) 196, 331, 846, 851, 1464
Kpn2I TCCGGA 2 cut(s) 593, 1138
KpnI GGTACC 1 cut(s) 742
KroI GCCGGC 1 cut(s) 553
KroNI GCCGGC 1 cut(s) 555
Kzo9I GATC 3 cut(s) 1135, 1328, 1515
LmnI GCTCC 5 cut(s) 203, 802, 903, 929, 1590
Lsp1109I GCAGC 2 cut(s) 55, 1380
LweI GCATC 4 cut(s) 146, 1200, 1284, 1306
MaeI CTAG 2 cut(s) 515, 558
MaeII ACGT 6 cut(s) 602, 831, 1128, 1284, 1338, 1488
MaeIII GTNAC 5 cut(s) 649, 832, 992, 1280, 1484
MalI GATC 3 cut(s) 1137, 1330, 1517
MbiI CCGCTC 2 cut(s) 459, 898
MboI GATC 3 cut(s) 1135, 1328, 1515
MboII GAAGA 6 cut(s) 346, 394, 1267, 1492, 1603, 1618
MflI RGATCY 2 cut(s) 1135, 1328
MluCI AATT 1 cut(s) 1317
MlyI GAGTC 2 cut(s) 974, 1391
MmeI TCCRAC 1 cut(s) 1152
Mph1103I ATGCAT 1 cut(s) 1299
MroI TCCGGA 2 cut(s) 593, 1138
MroNI GCCGGC 1 cut(s) 553
MseI TTAA 3 cut(s) 581, 1320, 1575
MslI CAYNNNNRTG 2 cut(s) 839, 1302
MspA1I CMGCKG 1 cut(s) 166
MspR9I CCNGG 4 cut(s) 203, 271, 908, 1232
MteI GCGCNGCGC 1 cut(s) 849
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 2 cut(s) 851, 1466
MwoI GCNNNNNNNGC 7 cut(s) 636, 755, 859, 868, 1159, 1403, 1427
NaeI GCCGGC 1 cut(s) 555
NciI CCSGG 3 cut(s) 271, 908, 1232
NcoI CCATGG 1 cut(s) 750
NdeII GATC 3 cut(s) 1135, 1328, 1515
NgoMIV GCCGGC 1 cut(s) 553
NheI GCTAGC 1 cut(s) 557
NlaIII CATG 8 cut(s) 241, 268, 754, 844, 871, 1032, 1522, 1555
NmeAIII GCCGAG 1 cut(s) 988
NmuCI GTSAC 4 cut(s) 649, 832, 1280, 1484
NsiI ATGCAT 1 cut(s) 1299
NspI RCATGY 2 cut(s) 844, 871
PciI ACATGT 1 cut(s) 840
PcsI WCGNNNNNNNCGW 5 cut(s) 608, 1073, 1463, 1658, 1667
PdiI GCCGGC 1 cut(s) 555
PfeI GAWTC 8 cut(s) 341, 511, 529, 1142, 1360, 1399, 1609, 1645
PflFI GACNNNGTC 1 cut(s) 604
PinAI ACCGGT 1 cut(s) 923
PkrI GCNGC 7 cut(s) 70, 757, 850, 939, 1370, 1420, 1545
PleI GAGTC 2 cut(s) 973, 1390
PpsI GAGTC 2 cut(s) 973, 1390
PscI ACATGT 1 cut(s) 840
PsiI TTATAA 1 cut(s) 1346
Psp124BI GAGCTC 1 cut(s) 1587
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 3 cut(s) 232, 472, 1213
PspGI CCWGG 1 cut(s) 201
PspOMI GGGCCC 1 cut(s) 567
PsuI RGATCY 2 cut(s) 1135, 1328
PsyI GACNNNGTC 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 166
RsaI GTAC 7 cut(s) 242, 491, 644, 681, 698, 740, 1240
RsaNI GTAC 7 cut(s) 241, 490, 643, 680, 697, 739, 1239
RseI CAYNNNNRTG 2 cut(s) 839, 1302
SacI GAGCTC 1 cut(s) 1587
SalI GTCGAC 1 cut(s) 359
SaqAI TTAA 3 cut(s) 581, 1320, 1575
SatI GCNGC 7 cut(s) 69, 756, 849, 938, 1369, 1419, 1544
Sau3AI GATC 3 cut(s) 1135, 1328, 1515
ScaI AGTACT 1 cut(s) 491
SchI GAGTC 2 cut(s) 974, 1391
ScrFI CCNGG 4 cut(s) 203, 271, 908, 1232
SfaNI GCATC 4 cut(s) 146, 1200, 1284, 1306
SfcI CTRYAG 1 cut(s) 808
SgrAI CRCCGGYG 1 cut(s) 923
SinI GGWCC 5 cut(s) 4, 138, 259, 547, 675
SmiMI CAYNNNNRTG 2 cut(s) 839, 1302
Sse9I AATT 1 cut(s) 1317
SspMI CTAG 2 cut(s) 515, 558
SstI GAGCTC 1 cut(s) 1587
StyD4I CCNGG 4 cut(s) 201, 269, 906, 1230
StyI CCWWGG 4 cut(s) 654, 750, 1332, 1638
TaaI ACNGT 3 cut(s) 152, 992, 1567
TaiI ACGT 6 cut(s) 605, 834, 1131, 1287, 1341, 1491
TaqI TCGA 7 cut(s) 60, 154, 303, 360, 540, 792, 1598
TaqII GACCGA 1 cut(s) 1638
TasI AATT 1 cut(s) 1317
TatI WGTACW 3 cut(s) 489, 642, 1238
TauI GCSGC 5 cut(s) 758, 851, 940, 1421, 1546
TfiI GAWTC 8 cut(s) 341, 511, 529, 1142, 1360, 1399, 1609, 1645
Tru1I TTAA 3 cut(s) 581, 1320, 1575
Tru9I TTAA 3 cut(s) 581, 1320, 1575
TscAI CASTG 3 cut(s) 695, 765, 1161
TseFI GTSAC 4 cut(s) 649, 832, 1280, 1484
TseI GCWGC 2 cut(s) 68, 1368
Tsp45I GTSAC 4 cut(s) 649, 832, 1280, 1484
TspRI CASTG 3 cut(s) 695, 765, 1161
Tth111I GACNNNGTC 1 cut(s) 604
VneI GTGCAC 1 cut(s) 623
VpaK11BI GGWCC 5 cut(s) 4, 138, 259, 547, 675
XceI RCATGY 2 cut(s) 844, 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 757
XmiI GTMKAC 1 cut(s) 360
XspI CTAG 2 cut(s) 515, 558
ZraI GACGTC 1 cut(s) 603
ZrmI AGTACT 1 cut(s) 491
Zsp2I ATGCAT 1 cut(s) 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.