MD09G1283100.v1.1

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000677
Physical Location & Seq
Forward (+)
36079067 .. 36081044
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1283100.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGGAGCAGCTCAATCCCAGCCCTGCAAATCTCTGCCCTCTCACTCCCCTCACCCTGCTAGAAAGGGCAGCCATCGTCTACGGCGACTGCCCCTCCGTCGTCTACACCACCACCACCTACACGTGGTCCCAAACACACCGCCGATGCCTCCAGCTGGCCTCCTCCCTCTCATCCCTCCTCGGCATCAAACGCTCCCAAGTCGTCTCTGTAATCTCGCCCAACACCCTTGCCATGTACGAGCTCCACTTCGCCGTCCCCATGGCAGGAGCCATCCTCAACACCATCAACACCCGCCTCGACGCCCGCACCGTCTCCGTCATCCTCCGCCACAGCGAATCAAAACTCATCTTCGTCGACCACCTCTTTGCCTCTCTCGTACTGGAAGCCCTCTCGCTCTTCCCACCTCAGCTTCCTCGTCCCCAACTCGTCCTCATCACCGATCATCCGAAGGACGGATCAATTCCTACCGTCGATCATTTCATCGACACTTACGAGAGTCTAGTGCGAAAAGGGGATCCTTTGTTTAAGTGGGTCCGGCCGGTTTCCGAGTGGGACCCAATCGTTTTAAACTACACGTCCGGAACGACGTCGGCTCCGAAGGGGGTTGTGCATTGCCACAGAGCCGTTTACAACGTGGCCCTGAGTTCCCTCACGGACTGGTCCGTGCCCAAAAACTGCGTCTACCTATGGACCCTACCGATGTTCCACGCAAACGGGTGGTGCTTCCCGTGGGCCATGGCCGCCGTCGGCGGGACAAACATTTGCGTCCGCAAATTCGATGGCCCAAAAATCTACGAATTAATCGAAGATAACGGCGTCACCCACATGTGCGCCGCGCCCGTCGTGCTCAACATGCTCATCAACACCCGGAGGTCCGACCCGCTCCGAAACCCAGTTAACATTCTTACCGGTGGGGCTCCGCCGCCTTCGAGCGTCTTGCTCCGGGCCGAGTCGATCGGGTTCGTAGTGGGTCACGGGTACGGGATGACCGAGACAGCGGGGGTCGTGCTGTCATGCGCATGGAAGCCGGAGTGGAACAAACTCCCGGCGATGGAGCAGGCGAGGCTGAAAGCGAGGCAGGGAGTGATGACGATCGGCATGACGGAGGCGGATGTGGTGAATCCGGATTCAGGTTTGAGTGTGAATCGGGACGGGTTGGAAATCGGGGAGATAGTTTTGAGAGGCGGGTGCCTGATGCTCGGGTATCTGAAGGATGCAGCGGGAACTGCCAACTGCATGAAAGACGGCTGGTTTTATACAGGTGACGTGGGGGTGATGCATCCCGACGGCTATTTGGAAATCAAGGACAGATCCAAGGACGTTATTATAAGCGGGGGTGAGAACATGAGCAGCGTGGAGGTCGAGTCCGTGCTGTATGGGAACCCAGCTGTCAATGAGGCGGCGGTCGTGGCGAGGCCCGACGAGTTTTGGGGGGAGACGCCGTGCGCGTTCGTGAGCCTGAAGAGTGACGTCAGCCCAAAGCCAACGGAGAAGGAGATCATTGAGTATTGCAGGGGGAAGCTGCCGAGCTTCATGGTGCCAAAGACGGTGGTGATTAAGGACGAGCTTCCCAAGACTTCCACCGGGAAGATTCAGAAGTTTTTGCTGAGGGAGATTGCCAGAGGGATTGCGCTGTCTTCGTTATTTCCGCCGCTGGCGGCAGTGCCGGCGTCGCGGACGACAAATAGGATGTGCATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

568

Amino Acids

61.97

Weight (kDa)

6.86

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 21 - 403 2.8e-75 AMP-binding enzyme
AMP-binding_C PF13193 453 - 530 8.7e-25 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21530 AT1G21530 AT1G21540 AT1G77240
fragaria_vesca FvH4_1g27170 FvH4_1g27180
malus_domestica MD09G1283000.v1.1 MD09G1283100.v1.1 MD17G1272700.v1.1 MD17G1273900.v1.1
prunus_persica Prupe.3G011200_v2.0.a1 Prupe.3G011300_v2.0.a1
pyrus_communis pycom09g19090 pycom09g19100 pycom17g27150 pycom17g27180 pycom17g27190
rosa_chinensis RchiOBHm_Chr3g0487461 RchiOBHm_Chr3g0487511 RchiOBHm_Chr3g0487561 RchiOBHm_Chr3g0487571 RchiOBHm_Chr3g0487741 RchiOBHm_Chr4g0432931
rosa_laevigata RLG00000013740 RLG00000022982 RLG00000023000 RLG00000023001 RLG00000023003
rosa_multiflora Rmu_co8229999.1_g000001 Rmu_sc0001491.1_g000005 Rmu_sc0005285.1_g000013 Rmu_sc0005285.1_g000017 Rmu_sc0005285.1_g000018 Rmu_sc0007025.1_g000002 Rmu_sc0008132.1_g000007 Rmu_sc0008132.1_g000012 Rmu_sc0009101.1_g000001 Rmu_sc0009101.1_g000002 Rmu_sc0010073.1_g000016 Rmu_sc0011808.1_g000002 Rmu_sc0011808.1_g000006 Rmu_sc0032698.1_g000001 Rmu_ssc0000018.1_g000065
rosa_roxburghii Rroxscaffold_6G00394540 Rroxscaffold_6G00394550 Rroxscaffold_6G00394740 Rroxscaffold_6G00394780
rosa_rugosa Rorug03G0234100 Rorug03G0234100 Rorug03G0236100
rosa_samantha Rh3AG282200 Rh3AG282500 Rh3AG282800 Rh3AG284200 Rh3BG317800 Rh3BG318300 Rh3BG320600 Rh3CG316300 Rh3CG316500 Rh3CG316700 Rh3CG319100 Rh3CG319200 Rh3CG319300 Rh3DG313900 Rh3DG314000 Rh3DG314300 Rh3DG314400 Rh3DG317800
rosa_wichuraiana Rw0G009700 Rw3G024990 Rw3G025000 Rw3G025010 Rw3G025020 Rw3G025200 Rw3G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1328
AatII GACGTC 2 cut(s) 590, 1473
Acc16I TGCGCA 1 cut(s) 1018
AccB1I GGYRCC 2 cut(s) 1188, 1537
AccBSI CCGCTC 1 cut(s) 883
AccI GTMKAC 4 cut(s) 78, 102, 354, 681
AccII CGCG 3 cut(s) 836, 1448, 1675
AccIII TCCGGA 2 cut(s) 578, 1123
AclWI GGATC 4 cut(s) 463, 509, 522, 1305
AcoI YGGCCR 2 cut(s) 536, 738
AcsI RAATTY 1 cut(s) 773
AcuI CTGAAG 2 cut(s) 1229, 1481
AcvI CACGTG 1 cut(s) 123
AcyI GRCGYC 6 cut(s) 300, 587, 816, 1439, 1470, 1670
AfaI GTAC 3 cut(s) 236, 378, 980
AfiI CCNNNNNNNGG 7 cut(s) 123, 154, 263, 452, 750, 1051, 1130
AflIII ACRYGT 3 cut(s) 120, 573, 825
AgeI ACCGGT 1 cut(s) 908
AjiI CACGTC 2 cut(s) 576, 1267
AluBI AGCT 8 cut(s) 10, 154, 241, 409, 1388, 1522, 1530, 1567
AluI AGCT 8 cut(s) 10, 154, 241, 409, 1388, 1522, 1530, 1567
Alw21I GWGCWC 2 cut(s) 243, 849
Alw26I GTCTC 4 cut(s) 208, 316, 986, 1430
AlwI GGATC 4 cut(s) 463, 509, 522, 1305
Ama87I CYCGRG 1 cut(s) 1199
Aor13HI TCCGGA 2 cut(s) 578, 1123
AoxI GGCC 8 cut(s) 156, 536, 636, 732, 738, 781, 945, 1415
ApeKI GCWGC 5 cut(s) 7, 68, 1217, 1350, 1522
ApoI RAATTY 1 cut(s) 773
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 663, 695
AseI ATTAAT 1 cut(s) 800
AsiGI ACCGGT 1 cut(s) 908
AspLEI GCGC 5 cut(s) 833, 838, 1019, 1448, 1633
AsuC2I CCSGG 4 cut(s) 868, 944, 1046, 1585
AsuHPI GGTGA 8 cut(s) 43, 427, 811, 1129, 1274, 1285, 1349, 1564
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 6 cut(s) 126, 532, 553, 660, 690, 873
BaeGI GKGCMC 1 cut(s) 669
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 2 cut(s) 1188, 1537
BanII GRGCYC 2 cut(s) 243, 919
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 1629
Bbv12I GWGCWC 2 cut(s) 243, 849
BbvCI CCTCAGC 2 cut(s) 405, 1607
BbvI GCAGC 5 cut(s) 19, 80, 1229, 1362, 1509
BccI CCATC 5 cut(s) 80, 278, 290, 773, 1045
BceAI ACGGC 8 cut(s) 97, 236, 608, 728, 829, 1261, 1303, 1426
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BcnI CCSGG 4 cut(s) 868, 944, 1046, 1585
BcoDI GTCTC 4 cut(s) 208, 316, 986, 1430
BfaI CTAG 2 cut(s) 59, 500
Bme1390I CCNGG 4 cut(s) 868, 944, 1046, 1585
Bme18I GGWCC 6 cut(s) 126, 532, 553, 660, 690, 873
BmeT110I CYCGRG 1 cut(s) 1199
BmgBI CACGTC 2 cut(s) 576, 1267
BmrFI CCNGG 4 cut(s) 868, 944, 1046, 1585
BmrI ACTGGG 1 cut(s) 887
BmsI GCATC 6 cut(s) 134, 192, 1185, 1204, 1266, 1288
BmuI ACTGGG 1 cut(s) 887
BpiI GAAGAC 1 cut(s) 1629
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 134
Bpu10I CCTNAGC 2 cut(s) 405, 1607
BpuMI CCSGG 4 cut(s) 868, 944, 1046, 1585
BsaAI YACGTR 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 1091
BsaHI GRCGYC 6 cut(s) 300, 587, 816, 1439, 1470, 1670
BsaJI CCNNGG 5 cut(s) 178, 258, 728, 735, 1314
BsaWI WCCGGW 3 cut(s) 578, 908, 1123
BsaXI ACNNNNNCTCC 8 cut(s) 77, 107, 577, 607, 1349, 1379, 1427, 1457
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 154, 263, 452, 750, 1051, 1130
Bse118I RCCGGY 3 cut(s) 538, 908, 1666
Bse1I ACTGG 3 cut(s) 384, 662, 893
Bse3DI GCAATG 2 cut(s) 610, 1695
Bse8I GATNNNNATC 1 cut(s) 1091
BseAI TCCGGA 2 cut(s) 578, 1123
BseDI CCNNGG 5 cut(s) 178, 258, 728, 735, 1314
BseGI GGATG 9 cut(s) 170, 270, 318, 442, 990, 1117, 1219, 1279, 1695
BseJI GATNNNNATC 1 cut(s) 1091
BseLI CCNNNNNNNGG 7 cut(s) 123, 154, 263, 452, 750, 1051, 1130
BseMI GCAATG 2 cut(s) 610, 1695
BseMII CTCAG 3 cut(s) 419, 632, 1598
BseNI ACTGG 3 cut(s) 384, 662, 893
BseRI GAGGAG 2 cut(s) 151, 167
BseSI GKGCMC 1 cut(s) 669
BseX3I CGGCCG 1 cut(s) 536
BseXI GCAGC 5 cut(s) 19, 80, 1229, 1362, 1509
BseYI CCCAGC 2 cut(s) 17, 1384
Bsh1236I CGCG 3 cut(s) 836, 1448, 1675
Bsh1285I CGRYCG 4 cut(s) 539, 957, 1095, 1407
BshFI GGCC 8 cut(s) 158, 538, 638, 734, 740, 783, 947, 1417
BshNI GGYRCC 2 cut(s) 1188, 1537
BshTI ACCGGT 1 cut(s) 908
BsiEI CGRYCG 4 cut(s) 539, 957, 1095, 1407
BsiHKAI GWGCWC 2 cut(s) 243, 849
BsiHKCI CYCGRG 1 cut(s) 1199
BslFI GGGAC 6 cut(s) 112, 239, 402, 566, 766, 1163
BslI CCNNNNNNNGG 7 cut(s) 123, 154, 263, 452, 750, 1051, 1130
BsmAI GTCTC 4 cut(s) 208, 316, 986, 1430
BsmBI CGTCTC 3 cut(s) 208, 316, 1430
BsmFI GGGAC 6 cut(s) 112, 239, 402, 566, 766, 1163
BsnI GGCC 8 cut(s) 158, 538, 638, 734, 740, 783, 947, 1417
BsoBI CYCGRG 1 cut(s) 1199
Bsp1286I GDGCHC 4 cut(s) 243, 669, 849, 919
Bsp13I TCCGGA 2 cut(s) 578, 1123
Bsp143I GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
Bsp19I CCATGG 2 cut(s) 258, 735
BspANI GGCC 8 cut(s) 158, 538, 638, 734, 740, 783, 947, 1417
BspCNI CTCAG 3 cut(s) 418, 633, 1599
BspEI TCCGGA 2 cut(s) 578, 1123
BspFNI CGCG 3 cut(s) 836, 1448, 1675
BspPI GGATC 4 cut(s) 463, 509, 522, 1305
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 2 cut(s) 1188, 1537
BsrBI CCGCTC 1 cut(s) 883
BsrDI GCAATG 2 cut(s) 610, 1695
BsrFI RCCGGY 3 cut(s) 538, 908, 1666
BsrI ACTGG 3 cut(s) 384, 662, 893
BssAI RCCGGY 3 cut(s) 538, 908, 1666
BssECI CCNNGG 5 cut(s) 178, 258, 728, 735, 1314
BssMI GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
BssNI GRCGYC 6 cut(s) 300, 587, 816, 1439, 1470, 1670
BssT1I CCWWGG 3 cut(s) 258, 735, 1314
Bst4CI ACNGT 3 cut(s) 310, 469, 1549
Bst6I CTCTTC 2 cut(s) 401, 1457
BstACI GRCGYC 6 cut(s) 300, 587, 816, 1439, 1470, 1670
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 5 cut(s) 156, 304, 1059, 1656, 1668
BstDEI CTNAG 3 cut(s) 405, 641, 1607
BstDSI CCRYGG 3 cut(s) 258, 728, 735
BstF5I GGATG 9 cut(s) 170, 270, 318, 442, 990, 1117, 1219, 1279, 1695
BstFNI CGCG 3 cut(s) 836, 1448, 1675
BstHHI GCGC 5 cut(s) 833, 838, 1019, 1448, 1633
BstKTI GATC 8 cut(s) 442, 458, 475, 517, 957, 1095, 1313, 1500
BstMAI GTCTC 4 cut(s) 208, 316, 986, 1430
BstMBI GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
BstMCI CGRYCG 4 cut(s) 539, 957, 1095, 1407
BstMWI GCNNNNNNNGC 9 cut(s) 189, 740, 844, 853, 1063, 1226, 1409, 1667, 1672
BstNSI RCATGY 2 cut(s) 829, 856
BstSCI CCNGG 4 cut(s) 866, 942, 1044, 1583
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 3 cut(s) 836, 1448, 1675
BstV1I GCAGC 5 cut(s) 19, 80, 1229, 1362, 1509
BstV2I GAAGAC 1 cut(s) 1629
BstX2I RGATCY 2 cut(s) 514, 1310
BstYI RGATCY 2 cut(s) 514, 1310
BstZI CGGCCG 1 cut(s) 536
BsuRI GGCC 8 cut(s) 158, 538, 638, 734, 740, 783, 947, 1417
BtgI CCRYGG 3 cut(s) 258, 728, 735
BtgZI GCGATG 1 cut(s) 1064
BtrI CACGTC 2 cut(s) 576, 1267
BtsCI GGATG 9 cut(s) 170, 270, 318, 442, 990, 1117, 1219, 1279, 1695
BtsI GCAGTG 1 cut(s) 1668
BtsIMutI CAGTG 1 cut(s) 1668
Cac8I GCNNGC 5 cut(s) 156, 304, 1059, 1656, 1668
CfoI GCGC 5 cut(s) 833, 838, 1019, 1448, 1633
Cfr10I RCCGGY 3 cut(s) 538, 908, 1666
CseI GACGC 7 cut(s) 308, 667, 754, 805, 922, 1447, 1659
Csp6I GTAC 3 cut(s) 235, 377, 979
CspAI ACCGGT 1 cut(s) 908
CspCI CAANNNNNGTGG 2 cut(s) 1530, 1565
CviQI GTAC 3 cut(s) 235, 377, 979
DdeI CTNAG 3 cut(s) 405, 641, 1607
DpnI GATC 8 cut(s) 441, 457, 474, 516, 956, 1094, 1312, 1499
DpnII GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
DraI TTTAAA 1 cut(s) 567
EaeI YGGCCR 2 cut(s) 536, 738
EagI CGGCCG 1 cut(s) 536
Eam1104I CTCTTC 2 cut(s) 401, 1457
EarI CTCTTC 2 cut(s) 401, 1457
EciI GGCGGA 4 cut(s) 314, 909, 1124, 1638
Ecl136II GAGCTC 1 cut(s) 241
EclXI CGGCCG 1 cut(s) 536
Eco130I CCWWGG 3 cut(s) 258, 735, 1314
Eco24I GRGCYC 2 cut(s) 243, 919
Eco47I GGWCC 6 cut(s) 126, 532, 553, 660, 690, 873
Eco52I CGGCCG 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 241
Eco57I CTGAAG 2 cut(s) 1229, 1481
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 1199
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 553
EcoT14I CCWWGG 3 cut(s) 258, 735, 1314
EcoT22I ATGCAT 1 cut(s) 1281
EcoT38I GRGCYC 2 cut(s) 243, 919
ErhI CCWWGG 3 cut(s) 258, 735, 1314
Esp3I CGTCTC 3 cut(s) 208, 316, 1430
FaqI GGGAC 6 cut(s) 112, 239, 402, 566, 766, 1163
FauI CCCGC 8 cut(s) 299, 311, 743, 888, 991, 1178, 1213, 1325
FblI GTMKAC 4 cut(s) 78, 102, 354, 681
FokI GGATG 8 cut(s) 157, 257, 305, 429, 997, 1124, 1226, 1266
FriOI GRGCYC 2 cut(s) 243, 919
FspAI RTGCGCAY 1 cut(s) 1018
FspBI CTAG 2 cut(s) 59, 500
FspI TGCGCA 1 cut(s) 1018
GlaI GCGC 5 cut(s) 832, 837, 1018, 1447, 1632
GsaI CCCAGC 2 cut(s) 21, 1388
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 8 cut(s) 158, 538, 638, 734, 740, 783, 947, 1417
HgaI GACGC 7 cut(s) 308, 667, 754, 805, 922, 1447, 1659
HhaI GCGC 5 cut(s) 833, 838, 1019, 1448, 1633
Hin1I GRCGYC 6 cut(s) 300, 587, 816, 1439, 1470, 1670
Hin6I GCGC 5 cut(s) 831, 836, 1017, 1446, 1631
HinP1I GCGC 5 cut(s) 831, 836, 1017, 1446, 1631
HincII GTYRAC 2 cut(s) 355, 898
HindII GTYRAC 2 cut(s) 355, 898
HinfI GANTC 8 cut(s) 335, 496, 950, 1120, 1127, 1144, 1364, 1591
HpaI GTTAAC 1 cut(s) 898
HphI GGTGA 8 cut(s) 43, 427, 811, 1129, 1274, 1285, 1349, 1564
Hpy166II GTNNAC 6 cut(s) 79, 103, 355, 628, 682, 898
Hpy188I TCNGA 7 cut(s) 447, 547, 597, 877, 887, 1209, 1596
Hpy188III TCNNGA 5 cut(s) 579, 1124, 1148, 1283, 1453
Hpy8I GTNNAC 6 cut(s) 79, 103, 355, 628, 682, 898
HpyAV CCTTC 5 cut(s) 442, 592, 936, 1204, 1486
HpyCH4III ACNGT 3 cut(s) 310, 469, 1549
HpyCH4IV ACGT 7 cut(s) 122, 575, 587, 633, 1266, 1320, 1470
HpyCH4V TGCA 8 cut(s) 26, 610, 1217, 1236, 1279, 1512, 1695, 1700
HpyF10VI GCNNNNNNNGC 9 cut(s) 189, 740, 844, 853, 1063, 1226, 1409, 1667, 1672
HpyF3I CTNAG 3 cut(s) 405, 641, 1607
HpySE526I ACGT 7 cut(s) 122, 575, 587, 633, 1266, 1320, 1470
Hsp92I GRCGYC 6 cut(s) 300, 587, 816, 1439, 1470, 1670
HspAI GCGC 5 cut(s) 831, 836, 1017, 1446, 1631
KflI GGGWCCC 1 cut(s) 553
Kpn2I TCCGGA 2 cut(s) 578, 1123
KroI GCCGGC 1 cut(s) 1666
KroNI GCCGGC 1 cut(s) 1668
KspAI GTTAAC 1 cut(s) 898
Kzo9I GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 9 cut(s) 4, 197, 246, 266, 598, 888, 922, 945, 1054
Lsp1109I GCAGC 5 cut(s) 19, 80, 1229, 1362, 1509
LweI GCATC 6 cut(s) 134, 192, 1185, 1204, 1266, 1288
MaeI CTAG 2 cut(s) 59, 500
MaeII ACGT 7 cut(s) 122, 575, 587, 633, 1266, 1320, 1470
MaeIII GTNAC 4 cut(s) 817, 971, 1262, 1466
MalI GATC 8 cut(s) 441, 457, 474, 516, 956, 1094, 1312, 1499
MbiI CCGCTC 1 cut(s) 883
MboI GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
MboII GAAGA 6 cut(s) 340, 388, 818, 1474, 1600, 1629
MflI RGATCY 2 cut(s) 514, 1310
MhlI GDGCHC 4 cut(s) 243, 669, 849, 919
MluCI AATT 3 cut(s) 459, 773, 797
MlyI GAGTC 3 cut(s) 505, 959, 1373
MmeI TCCRAC 2 cut(s) 900, 1137
Mph1103I ATGCAT 1 cut(s) 1281
MroI TCCGGA 2 cut(s) 578, 1123
MroNI GCCGGC 1 cut(s) 1666
MseI TTAA 5 cut(s) 525, 566, 800, 897, 1557
MslI CAYNNNNRTG 2 cut(s) 824, 1018
MspA1I CMGCKG 5 cut(s) 154, 998, 1220, 1388, 1654
MspR9I CCNGG 4 cut(s) 868, 944, 1046, 1585
MteI GCGCNGCGC 1 cut(s) 834
MvnI CGCG 3 cut(s) 836, 1448, 1675
MwoI GCNNNNNNNGC 9 cut(s) 189, 740, 844, 853, 1063, 1226, 1409, 1667, 1672
NaeI GCCGGC 1 cut(s) 1668
NciI CCSGG 4 cut(s) 868, 944, 1046, 1585
NcoI CCATGG 2 cut(s) 258, 735
NdeII GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
NgoMIV GCCGGC 1 cut(s) 1666
NmeAIII GCCGAG 3 cut(s) 159, 973, 1551
NmuCI GTSAC 4 cut(s) 817, 971, 1262, 1466
NsbI TGCGCA 1 cut(s) 1018
NsiI ATGCAT 1 cut(s) 1281
NspI RCATGY 2 cut(s) 829, 856
PciI ACATGT 1 cut(s) 825
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 8 cut(s) 81, 306, 489, 581, 593, 801, 987, 1445
PdiI GCCGGC 1 cut(s) 1668
PfeI GAWTC 5 cut(s) 335, 1120, 1127, 1144, 1591
PinAI ACCGGT 1 cut(s) 908
Ple19I CGATCG 2 cut(s) 957, 1095
PleI GAGTC 3 cut(s) 504, 958, 1372
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
PpsI GAGTC 3 cut(s) 504, 958, 1372
Ppu21I YACGTR 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 553
PscI ACATGT 1 cut(s) 825
PshBI ATTAAT 1 cut(s) 800
PsiI TTATAA 1 cut(s) 1328
Psp124BI GAGCTC 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 553
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 2 cut(s) 17, 1384
PspPPI RGGWCCY 1 cut(s) 553
PsuI RGATCY 2 cut(s) 514, 1310
PvuI CGATCG 2 cut(s) 957, 1095
PvuII CAGCTG 2 cut(s) 154, 1388
RsaI GTAC 3 cut(s) 236, 378, 980
RsaNI GTAC 3 cut(s) 235, 377, 979
RseI CAYNNNNRTG 2 cut(s) 824, 1018
SacI GAGCTC 1 cut(s) 243
SalI GTCGAC 1 cut(s) 353
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 5 cut(s) 525, 566, 800, 897, 1557
Sau3AI GATC 8 cut(s) 439, 455, 472, 514, 954, 1092, 1310, 1497
SchI GAGTC 3 cut(s) 505, 959, 1373
ScrFI CCNGG 4 cut(s) 868, 944, 1046, 1585
SduI GDGCHC 4 cut(s) 243, 669, 849, 919
SfaNI GCATC 6 cut(s) 134, 192, 1185, 1204, 1266, 1288
SinI GGWCC 6 cut(s) 126, 532, 553, 660, 690, 873
SmiMI CAYNNNNRTG 2 cut(s) 824, 1018
Sse9I AATT 3 cut(s) 459, 773, 797
SspMI CTAG 2 cut(s) 59, 500
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 4 cut(s) 866, 942, 1044, 1583
StyI CCWWGG 3 cut(s) 258, 735, 1314
TaaI ACNGT 3 cut(s) 310, 469, 1549
TaiI ACGT 7 cut(s) 125, 578, 590, 636, 1269, 1323, 1473
TaqI TCGA 9 cut(s) 297, 354, 471, 483, 777, 804, 929, 953, 1362
TaqII GACCGA 1 cut(s) 1004
TasI AATT 3 cut(s) 459, 773, 797
TauI GCSGC 6 cut(s) 743, 836, 925, 1403, 1654, 1661
TfiI GAWTC 5 cut(s) 335, 1120, 1127, 1144, 1591
Tru1I TTAA 5 cut(s) 525, 566, 800, 897, 1557
Tru9I TTAA 5 cut(s) 525, 566, 800, 897, 1557
TscAI CASTG 1 cut(s) 1668
TseFI GTSAC 4 cut(s) 817, 971, 1262, 1466
TseI GCWGC 5 cut(s) 7, 68, 1217, 1350, 1522
Tsp45I GTSAC 4 cut(s) 817, 971, 1262, 1466
TspDTI ATGAA 3 cut(s) 469, 1253, 1522
TspGWI ACGGA 8 cut(s) 85, 304, 468, 652, 668, 1118, 1357, 1502
TspRI CASTG 1 cut(s) 1668
VpaK11BI GGWCC 6 cut(s) 126, 532, 553, 660, 690, 873
VspI ATTAAT 1 cut(s) 800
XapI RAATTY 1 cut(s) 773
XceI RCATGY 2 cut(s) 829, 856
XmiI GTMKAC 4 cut(s) 78, 102, 354, 681
XspI CTAG 2 cut(s) 59, 500
ZraI GACGTC 2 cut(s) 588, 1471
Zsp2I ATGCAT 1 cut(s) 1281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.