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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1245 |
| Acc36I | ACCTGC | 2 cut(s) | 29, 1063 |
| AccBSI | CCGCTC | 1 cut(s) | 392 |
| AccI | GTMKAC | 2 cut(s) | 141, 417 |
| AciI | CCGC | 4 cut(s) | 59, 392, 674, 1067 |
| AclWI | GGATC | 1 cut(s) | 492 |
| AcoI | YGGCCR | 1 cut(s) | 393 |
| AcsI | RAATTY | 7 cut(s) | 506, 805, 813, 989, 1019, 1073, 1208 |
| AcuI | CTGAAG | 1 cut(s) | 998 |
| AcyI | GRCGYC | 1 cut(s) | 1242 |
| AfaI | GTAC | 3 cut(s) | 592, 832, 943 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 617 |
| AflII | CTTAAG | 1 cut(s) | 875 |
| AgsI | TTSAA | 7 cut(s) | 511, 533, 662, 935, 1040, 1078, 1190 |
| AhlI | ACTAGT | 1 cut(s) | 604 |
| AluBI | AGCT | 5 cut(s) | 50, 260, 377, 559, 647 |
| AluI | AGCT | 5 cut(s) | 50, 260, 377, 559, 647 |
| Alw26I | GTCTC | 3 cut(s) | 56, 83, 1249 |
| AlwI | GGATC | 1 cut(s) | 492 |
| AoxI | GGCC | 2 cut(s) | 393, 1128 |
| ApeKI | GCWGC | 2 cut(s) | 50, 1106 |
| ApoI | RAATTY | 7 cut(s) | 506, 805, 813, 989, 1019, 1073, 1208 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 986, 1018, 1071, 1103 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 726 |
| AspLEI | GCGC | 1 cut(s) | 623 |
| AspS9I | GGNCC | 1 cut(s) | 949 |
| AsuC2I | CCSGG | 1 cut(s) | 977 |
| AsuHPI | GGTGA | 4 cut(s) | 91, 160, 657, 1218 |
| AsuII | TTCGAA | 1 cut(s) | 32 |
| AvaII | GGWCC | 1 cut(s) | 949 |
| BbsI | GAAGAC | 1 cut(s) | 104 |
| BbvCI | CCTCAGC | 2 cut(s) | 261, 1218 |
| BbvI | GCAGC | 2 cut(s) | 37, 1118 |
| BciVI | GTATCC | 1 cut(s) | 331 |
| BcnI | CCSGG | 1 cut(s) | 977 |
| BcoDI | GTCTC | 3 cut(s) | 56, 83, 1249 |
| BcuI | ACTAGT | 1 cut(s) | 604 |
| BfaI | CTAG | 1 cut(s) | 605 |
| BfrI | CTTAAG | 1 cut(s) | 875 |
| BfuAI | ACCTGC | 2 cut(s) | 29, 1063 |
| BfuI | GTATCC | 1 cut(s) | 331 |
| BglII | AGATCT | 1 cut(s) | 842 |
| BisI | GCNGC | 3 cut(s) | 51, 393, 1107 |
| BlsI | GCNGC | 3 cut(s) | 52, 394, 1108 |
| BmcAI | AGTACT | 1 cut(s) | 943 |
| Bme1390I | CCNGG | 1 cut(s) | 977 |
| Bme18I | GGWCC | 1 cut(s) | 949 |
| BmgT120I | GGNCC | 1 cut(s) | 949 |
| BmrFI | CCNGG | 1 cut(s) | 977 |
| BmrI | ACTGGG | 1 cut(s) | 1006 |
| BmsI | GCATC | 3 cut(s) | 193, 484, 1126 |
| BmuI | ACTGGG | 1 cut(s) | 1006 |
| BpiI | GAAGAC | 1 cut(s) | 104 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 543, 575 |
| BpmI | CTGGAG | 1 cut(s) | 461 |
| Bpu10I | CCTNAGC | 3 cut(s) | 177, 261, 1218 |
| Bpu14I | TTCGAA | 1 cut(s) | 32 |
| BpuMI | CCSGG | 1 cut(s) | 977 |
| BsaBI | GATNNNNATC | 1 cut(s) | 692 |
| BsaHI | GRCGYC | 1 cut(s) | 1242 |
| BsaJI | CCNNGG | 1 cut(s) | 975 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 434, 464 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 617 |
| Bse1I | ACTGG | 5 cut(s) | 467, 758, 1001, 1038, 1255 |
| Bse8I | GATNNNNATC | 1 cut(s) | 692 |
| BseDI | CCNNGG | 1 cut(s) | 975 |
| BseGI | GGATG | 3 cut(s) | 208, 235, 1075 |
| BseJI | GATNNNNATC | 1 cut(s) | 692 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 617 |
| BseMII | CTCAG | 7 cut(s) | 80, 252, 333, 468, 865, 936, 1232 |
| BseNI | ACTGG | 5 cut(s) | 467, 758, 1001, 1038, 1255 |
| BseRI | GAGGAG | 6 cut(s) | 101, 125, 185, 239, 248, 440 |
| BseXI | GCAGC | 2 cut(s) | 37, 1118 |
| BseYI | CCCAGC | 1 cut(s) | 643 |
| BshFI | GGCC | 2 cut(s) | 395, 1130 |
| BsiSI | CCGG | 1 cut(s) | 977 |
| BslFI | GGGAC | 3 cut(s) | 593, 626, 1243 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 617 |
| BsmAI | GTCTC | 3 cut(s) | 56, 83, 1249 |
| BsmBI | CGTCTC | 2 cut(s) | 83, 1249 |
| BsmFI | GGGAC | 3 cut(s) | 593, 626, 1243 |
| BsnI | GGCC | 2 cut(s) | 395, 1130 |
| Bsp119I | TTCGAA | 1 cut(s) | 32 |
| Bsp1407I | TGTACA | 1 cut(s) | 590 |
| Bsp143I | GATC | 4 cut(s) | 184, 484, 842, 868 |
| BspACI | CCGC | 4 cut(s) | 59, 392, 674, 1067 |
| BspANI | GGCC | 2 cut(s) | 395, 1130 |
| BspCNI | CTCAG | 7 cut(s) | 79, 253, 334, 469, 864, 937, 1231 |
| BspHI | TCATGA | 1 cut(s) | 1291 |
| BspMI | ACCTGC | 2 cut(s) | 29, 1063 |
| BspPI | GGATC | 1 cut(s) | 492 |
| BspQI | GCTCTTC | 1 cut(s) | 402 |
| BspT104I | TTCGAA | 1 cut(s) | 32 |
| BspTI | CTTAAG | 1 cut(s) | 875 |
| BsrBI | CCGCTC | 1 cut(s) | 392 |
| BsrGI | TGTACA | 1 cut(s) | 590 |
| BsrI | ACTGG | 5 cut(s) | 467, 758, 1001, 1038, 1255 |
| BssECI | CCNNGG | 1 cut(s) | 975 |
| BssMI | GATC | 4 cut(s) | 184, 484, 842, 868 |
| BssNI | GRCGYC | 1 cut(s) | 1242 |
| Bst6I | CTCTTC | 5 cut(s) | 11, 162, 192, 402, 1035 |
| BstACI | GRCGYC | 1 cut(s) | 1242 |
| BstAFI | CTTAAG | 1 cut(s) | 875 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1055 |
| BstAUI | TGTACA | 1 cut(s) | 590 |
| BstBI | TTCGAA | 1 cut(s) | 32 |
| BstC8I | GCNNGC | 1 cut(s) | 1182 |
| BstF5I | GGATG | 3 cut(s) | 208, 235, 1075 |
| BstHHI | GCGC | 1 cut(s) | 623 |
| BstKTI | GATC | 4 cut(s) | 187, 487, 845, 871 |
| BstMAI | GTCTC | 3 cut(s) | 56, 83, 1249 |
| BstMBI | GATC | 4 cut(s) | 184, 484, 842, 868 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 56, 1055, 1064, 1115, 1136 |
| BstSCI | CCNGG | 1 cut(s) | 975 |
| BstV1I | GCAGC | 2 cut(s) | 37, 1118 |
| BstV2I | GAAGAC | 1 cut(s) | 104 |
| BstX2I | RGATCY | 1 cut(s) | 842 |
| BstYI | RGATCY | 1 cut(s) | 842 |
| BsuI | GTATCC | 1 cut(s) | 331 |
| BsuRI | GGCC | 2 cut(s) | 395, 1130 |
| BtsCI | GGATG | 3 cut(s) | 208, 235, 1075 |
| BtsIMutI | CAGTG | 1 cut(s) | 474 |
| BveI | ACCTGC | 2 cut(s) | 29, 1063 |
| Cac8I | GCNNGC | 1 cut(s) | 1182 |
| CciI | TCATGA | 1 cut(s) | 1291 |
| CfoI | GCGC | 1 cut(s) | 623 |
| Cfr13I | GGNCC | 1 cut(s) | 949 |
| CseI | GACGC | 1 cut(s) | 112 |
| Csp6I | GTAC | 3 cut(s) | 591, 831, 942 |
| CviAII | CATG | 3 cut(s) | 125, 638, 1292 |
| CviQI | GTAC | 3 cut(s) | 591, 831, 942 |
| DpnI | GATC | 4 cut(s) | 186, 486, 844, 870 |
| DpnII | GATC | 4 cut(s) | 184, 484, 842, 868 |
| EaeI | YGGCCR | 1 cut(s) | 393 |
| Eam1104I | CTCTTC | 5 cut(s) | 11, 162, 192, 402, 1035 |
| EarI | CTCTTC | 5 cut(s) | 11, 162, 192, 402, 1035 |
| EciI | GGCGGA | 1 cut(s) | 663 |
| Eco47I | GGWCC | 1 cut(s) | 949 |
| Eco57I | CTGAAG | 1 cut(s) | 998 |
| EcoRI | GAATTC | 3 cut(s) | 506, 805, 1073 |
| Esp3I | CGTCTC | 2 cut(s) | 83, 1249 |
| FaeI | CATG | 3 cut(s) | 128, 641, 1295 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 858, 890, 915, 947, 1169, 1201 |
| FaqI | GGGAC | 3 cut(s) | 593, 626, 1243 |
| FatI | CATG | 3 cut(s) | 124, 637, 1291 |
| FauNDI | CATATG | 1 cut(s) | 858 |
| FblI | GTMKAC | 2 cut(s) | 141, 417 |
| Fnu4HI | GCNGC | 3 cut(s) | 51, 393, 1107 |
| FokI | GGATG | 3 cut(s) | 215, 242, 1082 |
| Fsp4HI | GCNGC | 3 cut(s) | 51, 393, 1107 |
| FspBI | CTAG | 1 cut(s) | 605 |
| GlaI | GCGC | 1 cut(s) | 622 |
| GluI | GCNGC | 3 cut(s) | 51, 393, 1107 |
| GsaI | CCCAGC | 1 cut(s) | 647 |
| GsuI | CTGGAG | 1 cut(s) | 461 |
| HaeIII | GGCC | 2 cut(s) | 395, 1130 |
| HapII | CCGG | 1 cut(s) | 977 |
| HgaI | GACGC | 1 cut(s) | 112 |
| HhaI | GCGC | 1 cut(s) | 623 |
| Hin1I | GRCGYC | 1 cut(s) | 1242 |
| Hin1II | CATG | 3 cut(s) | 128, 641, 1295 |
| Hin6I | GCGC | 1 cut(s) | 621 |
| HinP1I | GCGC | 1 cut(s) | 621 |
| HincII | GTYRAC | 2 cut(s) | 119, 142 |
| HindII | GTYRAC | 2 cut(s) | 119, 142 |
| HinfI | GANTC | 6 cut(s) | 64, 115, 215, 238, 662, 693 |
| HpaII | CCGG | 1 cut(s) | 977 |
| HphI | GGTGA | 4 cut(s) | 91, 160, 657, 1218 |
| Hpy166II | GTNNAC | 4 cut(s) | 119, 142, 314, 418 |
| Hpy188I | TCNGA | 7 cut(s) | 69, 372, 682, 708, 771, 854, 970 |
| Hpy188III | TCNNGA | 7 cut(s) | 188, 242, 440, 530, 1016, 1124, 1292 |
| Hpy8I | GTNNAC | 4 cut(s) | 119, 142, 314, 418 |
| Hpy99I | CGWCG | 5 cut(s) | 97, 106, 143, 146, 306 |
| HpyAV | CCTTC | 9 cut(s) | 145, 328, 448, 542, 565, 687, 856, 905, 1233 |
| HpyCH4IV | ACGT | 1 cut(s) | 1242 |
| HpyCH4V | TGCA | 2 cut(s) | 461, 497 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 56, 1055, 1064, 1115, 1136 |
| HpySE526I | ACGT | 1 cut(s) | 1242 |
| Hsp92I | GRCGYC | 1 cut(s) | 1242 |
| Hsp92II | CATG | 3 cut(s) | 128, 641, 1295 |
| HspAI | GCGC | 1 cut(s) | 621 |
| Kzo9I | GATC | 4 cut(s) | 184, 484, 842, 868 |
| LguI | GCTCTTC | 1 cut(s) | 402 |
| LmnI | GCTCC | 1 cut(s) | 11 |
| Lsp1109I | GCAGC | 2 cut(s) | 37, 1118 |
| LweI | GCATC | 3 cut(s) | 193, 484, 1126 |
| MaeI | CTAG | 1 cut(s) | 605 |
| MaeII | ACGT | 1 cut(s) | 1242 |
| MalI | GATC | 4 cut(s) | 186, 486, 844, 870 |
| MbiI | CCGCTC | 1 cut(s) | 392 |
| MboI | GATC | 4 cut(s) | 184, 484, 842, 868 |
| MfeI | CAATTG | 1 cut(s) | 511 |
| MflI | RGATCY | 1 cut(s) | 842 |
| MluCI | AATT | 9 cut(s) | 488, 506, 511, 805, 813, 989, 1019, 1073, 1208 |
| MlyI | GAGTC | 2 cut(s) | 58, 124 |
| MroXI | GAANNNNTTC | 1 cut(s) | 726 |
| MseI | TTAA | 5 cut(s) | 348, 402, 876, 918, 1269 |
| MslI | CAYNNNNRTG | 1 cut(s) | 731 |
| MspA1I | CMGCKG | 1 cut(s) | 647 |
| MspCI | CTTAAG | 1 cut(s) | 875 |
| MspI | CCGG | 1 cut(s) | 977 |
| MspR9I | CCNGG | 1 cut(s) | 977 |
| MunI | CAATTG | 1 cut(s) | 511 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 56, 1055, 1064, 1115, 1136 |
| NciI | CCSGG | 1 cut(s) | 977 |
| NdeI | CATATG | 1 cut(s) | 858 |
| NdeII | GATC | 4 cut(s) | 184, 484, 842, 868 |
| NlaIII | CATG | 3 cut(s) | 128, 641, 1295 |
| NspV | TTCGAA | 1 cut(s) | 32 |
| PagI | TCATGA | 1 cut(s) | 1291 |
| PciSI | GCTCTTC | 1 cut(s) | 402 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 98 |
| PdmI | GAANNNNTTC | 1 cut(s) | 726 |
| PfeI | GAWTC | 4 cut(s) | 215, 238, 662, 693 |
| PkrI | GCNGC | 3 cut(s) | 52, 394, 1108 |
| PleI | GAGTC | 2 cut(s) | 58, 123 |
| PpsI | GAGTC | 2 cut(s) | 58, 123 |
| PspFI | CCCAGC | 1 cut(s) | 643 |
| PspPI | GGNCC | 1 cut(s) | 949 |
| PsuI | RGATCY | 1 cut(s) | 842 |
| PvuII | CAGCTG | 1 cut(s) | 647 |
| RsaI | GTAC | 3 cut(s) | 592, 832, 943 |
| RsaNI | GTAC | 3 cut(s) | 591, 831, 942 |
| RseI | CAYNNNNRTG | 1 cut(s) | 731 |
| SalI | GTCGAC | 1 cut(s) | 140 |
| SapI | GCTCTTC | 1 cut(s) | 402 |
| SaqAI | TTAA | 5 cut(s) | 348, 402, 876, 918, 1269 |
| SatI | GCNGC | 3 cut(s) | 51, 393, 1107 |
| Sau3AI | GATC | 4 cut(s) | 184, 484, 842, 868 |
| Sau96I | GGNCC | 1 cut(s) | 949 |
| ScaI | AGTACT | 1 cut(s) | 943 |
| SchI | GAGTC | 2 cut(s) | 58, 124 |
| ScrFI | CCNGG | 1 cut(s) | 977 |
| SfaNI | GCATC | 3 cut(s) | 193, 484, 1126 |
| SfuI | TTCGAA | 1 cut(s) | 32 |
| SgrDI | CGTCGACG | 1 cut(s) | 140 |
| SinI | GGWCC | 1 cut(s) | 949 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 731 |
| SmlI | CTYRAG | 1 cut(s) | 875 |
| SmoI | CTYRAG | 1 cut(s) | 875 |
| SpeI | ACTAGT | 1 cut(s) | 604 |
| Sse9I | AATT | 9 cut(s) | 488, 506, 511, 805, 813, 989, 1019, 1073, 1208 |
| SsiI | CCGC | 4 cut(s) | 59, 392, 674, 1067 |
| SspMI | CTAG | 1 cut(s) | 605 |
| StyD4I | CCNGG | 1 cut(s) | 975 |
| TaiI | ACGT | 1 cut(s) | 1245 |
| TaqI | TCGA | 7 cut(s) | 32, 141, 423, 1026, 1102, 1123, 1141 |
| TasI | AATT | 9 cut(s) | 488, 506, 511, 805, 813, 989, 1019, 1073, 1208 |
| TatI | WGTACW | 2 cut(s) | 590, 941 |
| TauI | GCSGC | 1 cut(s) | 395 |
| TfiI | GAWTC | 4 cut(s) | 215, 238, 662, 693 |
| Tru1I | TTAA | 5 cut(s) | 348, 402, 876, 918, 1269 |
| Tru9I | TTAA | 5 cut(s) | 348, 402, 876, 918, 1269 |
| TscAI | CASTG | 1 cut(s) | 474 |
| TseI | GCWGC | 2 cut(s) | 50, 1106 |
| TspRI | CASTG | 1 cut(s) | 474 |
| Vha464I | CTTAAG | 1 cut(s) | 875 |
| VpaK11BI | GGWCC | 1 cut(s) | 949 |
| XapI | RAATTY | 7 cut(s) | 506, 805, 813, 989, 1019, 1073, 1208 |
| XmiI | GTMKAC | 2 cut(s) | 141, 417 |
| XmnI | GAANNNNTTC | 1 cut(s) | 726 |
| XspI | CTAG | 1 cut(s) | 605 |
| ZraI | GACGTC | 1 cut(s) | 1243 |
| ZrmI | AGTACT | 1 cut(s) | 943 |