Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0000661

Physical Location & Seq

Genomic Coordinates Reverse (-)

74855296 .. 74863726

Gene Structure 8431 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0634700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3348 bp

ATGGAGGTGCTAGGGTTTAAAGCGAAGTCCAAAATAGCGCTGCTCGTTGTTCTCGTGCTGAGCTTTGGCCTTGCTGCGCTCTATGGCTTGTTGAAGCCGGTTTCGAACGGCTGTAACATGACGTATATGTATCCGACGTATATTCCGATACAGATGACTAACTCCGGTGCTGCACCGGCGGCGAAGTATGGGCTGTATTTGTACCACGAAGGCTGGAAGACGATTGATTTTAAGGAGCATGTACAGAAGCTAAGCGGCATTCCGGTGCTTTTTATTCCGGGGAATGGCGGGAGCTATAAGCAGGTGAGGTCATTGGCTGCGGAATCTGATAGGGCGTATAAAGTAGGTCCACTTGAGCGTACGTTTTATGAAGAAGCTCGGTTAACTCCGGAAGAGGGAGGGGAGGAGGATATGGATGCAGCTGGCTTTGAGTTGAAGAACCAATACGATTCGAGGTTGGACTGGTTCGCTGTGGATCTTGAAGGAGAACACTCTGCTATGGATAGTGCTGTACTTGAACAACACACACAATATGTTGTCCACTGCATTCACAGGATTTTGGATCAGTACGAGGAGTCTTTCAAGGCTAGAGAAAAGGAAGGAGCTGCAACCTCTGCGACTTTGCCAAAAAGTGTTATATTGGTTGGTCACTCTATGGGTGGTTTCGTTGCTAGAGCTGCAGTGGTCGACCCTCTTTTGAGAAAGTCAGCAGTTGAAACAATTATTACACTTTCCAGCCCACACCAACAACCTCCTGTGGCACTGCAGCCTTCCTATGGTCACTACTTTAGACATGTTAATCGAGAATGGAGAAAGGGATATGAGGTCCAAACAACTGCAGCAGGACATTACTTGTCTGGTCCTGCTCTCTCTAATGTTGTTGTCCTATCTATTTCTGGTGGTTATAATGATTACCAGGTAAGGACCAAGTATGAATCCCTTGATGGCATTGTGCCGCCTACCCATGGTTTTATGATCAGTAGTACCGGCATGAAAAATGTTTGGTTATCCATGGAACATCAAGTTATCTTATGGTGCAATCAATTAGTTATCCAAGTATCACACACTCTCCTTAGTTTGGCAGATTCCAGAACAGGCCAGCCTTTCTCTGACACACGAATAAGACTTGCAATATTCTCGAGAATGCTTCGTAGTGCAATACCACAAAGTTTCAATTGGAGGAAGCAATCGCAATTGTCTCAACAGTCAATGCATGCTCCAACTAGAGACGTGAAAGACAGAACTGAATCACTATACATCCCTGCTGCTTGTCCTAGCAATTTTCATTGGAGTGATGATGGCCTTGAGAGGGACTTATACATTCAGACAACCACCGTCACTGTTTTAGCCATGGATGGTCGAAGACGGTGGTTGGACATTCAAAAACTGGGATCCAATGGAAGAAGTCACTTCATGTTTGTGACCAACCTTGCTCCATGTTCTGGGGTTAGACTGCATCTATGGCCTGACAAAAGAAACTCAACTTCAGACTTGCCTGCATGTATAAGGATCCATGAAGTGACATCGAAGATGGTGCTCATTCCATCAGGACCAGCACCAAGACAGATTGAACCAGGAAGCCAAACAGAGCAAGCTCCCCCTTCAGCTATACTCTGCTTAGGACCCAAGGATATGCGTGGTTTCAGATTCCTGACCATCTCAGTTGCACCACGTCCGAGTATTTCAGGCAGACCTCCACCAGCAGTTTCCATGGCAGTTGGTCAGTTTTTTAATCCAGCGGAAGGGGAGAGAGAGATCTCTCCTTGGTCTCCTCCACTGTCTGGTTATTCTCACAAGGATTTATCTTGGAAGGAAGATCATCCCCTTGCTTTGAATCTATCATTCACAAGTAGTTTAGGCCTTCTGCCTGTAAAGTTCTCTTTGACGACCGCAGGTTGTGGAATAAAGAGTTCTGGACTTCCAGATGAACAGGCTGGAGATATTGATAATAGCAAGCTCTGTAAGCTTCGCTGTTTCCCACCAGTTGCATTTGCTTGGGATGAAACAGCAGGTCTCCACGTATTCACAGATTTGTATAGTGACAAAATTGTTGTTGATTCATCACCATCGCTTTGGAGTTCACCCATAAGTTCTGAGAAAACCTCTGTTATGTTGGTGGTAGATCCACATTGTTCATATAGCAGTGCGATGACTGTTTCCGTAACTGCAGTTGCCAGTAGGTTTTTGCTTGTGTATACTTCACAGATTATTGGTTTCTCCATTGTTGTTATCTTTTTTGCACTGATGCGGCAAGCATGTGCATGGGATCGTAACCTACCCATACCCTCGATGTTGAAGGCTCTGGAGTCCAATTTGAGATTCCCAATTCCATTTATCTACCTTGCTATTGCACCAATTGGTCTTTCTTTACTCGTTGGTTTTTGGATTTCTCAACCATCTCCTTCATTTGCTAGCTTCACCATTGTATCAGTTGTCTGCTATTTCCTTGCTAATGGCTTCATAGCCATTCTGATTTTGACATCCCAGTTCGCCTTTTATGGGGCTGCTCTGCTACATATTTTCATCAAGAAAAGGTTTCAATTGGGGGGAAAAACTTCTCAATGGTTTGTGAATATATCCTCTGGTTTCTGTTCATGGAAGGTGGTAAGGGTTATAAGAGCTAATCCACTAATGGTTACAGCACTTGCTGCAATTACTTTGGCATGCTTGGTTCATCCGGGTTTGGGTCTACTTGTTCTCTTGCTCTCTCATGCTTTGTGCTGTCATACTGCTCTTTGCAGTTTTTTGACAGCTTCATTTCGCAGCCATGCACTAAGACATGAATTATTTGATTATAAGAAAGACGGAAATGGTGGGCCTGACAAACTTACATTCAAACGTGATGGTATATCAAACCAGAATTTTACTTCTGAAGATACGTGCTCCAATAGCCCAGACTCTCCAAAAAGTTTTGGTGAAACTCAATTCGAGATATTCCATCATCGGCATGGGTTGTTTATTTTGCATCTTGTTGCAACGCTCATGTTTGGCCCATCATTTGTGACTTGGTTGGAGAGAATTGGGACGGATCACAGCTTTCCATGGGTCTTGGATTCAGCTCTTTCCACGGGTGTGATCCTTCATGGTATCTTCACTTCAAACCCTCAGTTCAATTCCTTCCTGGTTTCTTTCCCTCCAATCCGAAACCTAGAAGTGAGGATGCATTTACTGTACCTGTTTGCTGGATACTATTCCTATCTCTCTTCCCTGGCCTTGGCTCCATACAGAGAATTTTATGTTATGGCTGCTGTAGGCTATACTTCAATTGGTCTGACCCTCTTACAGAGATGGAACAAGGAGAAGGGAGATGCCCATTTTGTTAACCGAAAGCACTCTCACAGGCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005730 GO:0006950 GO:0007088 GO:0007346 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0009893 GO:0010286 GO:0010369 GO:0010468 GO:0010564 GO:0010604 GO:0010628 GO:0019222 GO:0031974 GO:0031981 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051726 GO:0051783 GO:0051983 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070013 GO:0080134 GO:0080135 GO:0098687 GO:1900034 GO:1901651

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1115

Amino Acids

123.8

Weight (kDa)

8.63

Isoelectric Point (pI)

41.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGAP1	PF07819	84 - 359	6.5e-82	PGAP1-like alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10010
fragaria_vesca	FvH4_6g03222 FvH4_6g03560 FvH4_6g03561 FvH4_6g03563 FvH4_6g03590 FvH4_6g03590 FvH4_6g09260
malus_domestica	MD04G1220200.v1.1 MD12G1240700.v1.1 MD15G1083500.v1.1
prunus_persica	Prupe.1G436000_v2.0.a1 Prupe.6G174800_v2.0.a1 Prupe.6G339200_v2.0.a1
pyrus_communis	pycom08g08260 pycom15g07800 pycom15g07810
rosa_chinensis	RchiOBHm_Chr3g0451551 RchiOBHm_Chr3g0451591 RchiOBHm_Chr3g0451621 RchiOBHm_Chr3g0452131 RchiOBHm_Chr3g0459621
rosa_laevigata	RLG00000025074 RLG00000025077 RLG00000025626 RLG00000029505 RLG00000035382 RLG00000035384
rosa_multiflora	Rmu_co8173672.1_g000001 Rmu_sc0000240.1_g000011 Rmu_sc0000240.1_g000014 Rmu_sc0004924.1_g000012 Rmu_sc0004924.1_g000020 Rmu_sc0004924.1_g000023 Rmu_sc0004924.1_g000025 Rmu_sc0004924.1_g000034 Rmu_sc0004924.1_g000035 Rmu_sc0009360.1_g000006 Rmu_sc0009360.1_g000008 Rmu_sc0009360.1_g000014 Rmu_sc0009360.1_g000047 Rmu_sc0011887.1_g000001 Rmu_sc0015119.1_g000001 Rmu_sc0023706.1_g000002
rosa_roxburghii	Rroxscaffold_164G00436050 Rroxscaffold_164G00436390 Rroxscaffold_164G00436450 Rroxscaffold_164G00436460 Rroxscaffold_4G00317080 Rroxscaffold_4G00317090 Rroxscaffold_6G00420140 Rroxscaffold_6G00428920
rosa_rugosa	Rorug02G0634200 Rorug02G0634500 Rorug02G0634700 Rorug02G0635400 Rorug02G0638500 Rorug03G0036600 Rorug03G0036700
rosa_samantha	Rh1BG103700 Rh1BG103800 Rh3AG040400 Rh3BG037900 Rh3BG038200 Rh3BG041900 Rh3BG097900 Rh3CG036600 Rh3CG040300 Rh3DG037300 Rh3DG037500 Rh3DG041200 Rh3DG099300
rosa_wichuraiana	Rw0G013830 Rw0G017580 Rw0G017600 Rw3G003070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	906, 2615, 2796
AarI	CACCTGC	1 cut(s)	292
Acc36I	ACCTGC	3 cut(s)	292, 1883, 2000
AccB7I	CCANNNNNTGG	2 cut(s)	1442, 1781
AccI	GTMKAC	3 cut(s)	687, 2195, 2689
AccIII	TCCGGA	1 cut(s)	388
AciI	CCGC	8 cut(s)	179, 255, 288, 320, 956, 1739, 1890, 2248
AcsI	RAATTY	2 cut(s)	2860, 3230
AcuI	CTGAAG	3 cut(s)	1470, 1587, 2892
AfaI	GTAC	7 cut(s)	203, 243, 361, 513, 569, 985, 3173
AfeI	AGCGCT	1 cut(s)	39
AflIII	ACRYGT	1 cut(s)	793
AjiI	CACGTC	2 cut(s)	1231, 1673
AjnI	CCWGG	4 cut(s)	915, 1573, 3120, 3207
AjuI	GAANNNNNNNTTGG	4 cut(s)	2303, 2335, 2347, 2379
AleI	CACNNNNGTG	1 cut(s)	3071
Alw21I	GWGCWC	2 cut(s)	1539, 2885
Alw26I	GTCTC	4 cut(s)	1203, 1221, 1773, 2018
AlwNI	CAGNNNCTG	1 cut(s)	3345
Ama87I	CYCGRG	1 cut(s)	1138
Aor13HI	TCCGGA	1 cut(s)	388
Aor51HI	AGCGCT	1 cut(s)	39
AoxI	GGCC	8 cut(s)	67, 1096, 1300, 1463, 1858, 2816, 2989, 3210
ApoI	RAATTY	2 cut(s)	2860, 3230
Asp700I	GAANNNNTTC	1 cut(s)	2554
AspLEI	GCGC	2 cut(s)	40, 79
AspS9I	GGNCC	8 cut(s)	347, 826, 860, 924, 1550, 1622, 2816, 2990
AsuC2I	CCSGG	2 cut(s)	279, 2679
AsuHPI	GGTGA	5 cut(s)	316, 2055, 2073, 2410, 2927
AsuII	TTCGAA	1 cut(s)	104
AsuNHI	GCTAGC	1 cut(s)	2411
AvaI	CYCGRG	1 cut(s)	1138
AvaII	GGWCC	6 cut(s)	347, 826, 860, 924, 1550, 1622
BaeI	ACNNNNGTAYC	2 cut(s)	2409, 2442
BamHI	GGATCC	2 cut(s)	1391, 1509
BarI	GAAGNNNNNNTAC	4 cut(s)	428, 460, 3079, 3111
BauI	CACGAG	1 cut(s)	53
BbsI	GAAGAC	2 cut(s)	224, 1369
Bbv12I	GWGCWC	2 cut(s)	1539, 2885
BceAI	ACGGC	1 cut(s)	124
BcgI	CGANNNNNNTGC	4 cut(s)	1119, 1153, 1516, 1550
BciT130I	CCWGG	4 cut(s)	917, 1575, 3122, 3209
BciVI	GTATCC	2 cut(s)	141, 3179
BclI	TGATCA	1 cut(s)	975
BcnI	CCSGG	2 cut(s)	279, 2679
BcoDI	GTCTC	4 cut(s)	1203, 1221, 1773, 2018
BfaI	CTAG	7 cut(s)	11, 588, 672, 1224, 1275, 2412, 3149
BfmI	CTRYAG	5 cut(s)	678, 764, 837, 2166, 3249
BfoI	RGCGCY	1 cut(s)	41
BfuAI	ACCTGC	3 cut(s)	292, 1883, 2000
BfuI	GTATCC	2 cut(s)	141, 3179
BglII	AGATCT	1 cut(s)	1755
BlpI	GCTNAGC	2 cut(s)	59, 251
Bme1390I	CCNGG	6 cut(s)	279, 917, 1575, 2679, 3122, 3209
Bme18I	GGWCC	6 cut(s)	347, 826, 860, 924, 1550, 1622
BmeT110I	CYCGRG	1 cut(s)	1138
BmgBI	CACGTC	2 cut(s)	1231, 1673
BmgT120I	GGNCC	8 cut(s)	347, 826, 860, 924, 1550, 1622, 2816, 2990
BmiI	GGNNCC	4 cut(s)	1393, 1511, 1624, 3219
BmrFI	CCNGG	6 cut(s)	279, 917, 1575, 2679, 3122, 3209
BmrI	ACTGGG	2 cut(s)	1397, 2479
BmsI	GCATC	6 cut(s)	406, 1465, 2235, 2974, 3150, 3298
BmtI	GCTAGC	1 cut(s)	2415
BmuI	ACTGGG	2 cut(s)	1397, 2479
BpiI	GAAGAC	2 cut(s)	224, 1369
BplI	GAGNNNNNCTC	2 cut(s)	2087, 2119
BpmI	CTGGAG	2 cut(s)	1956, 2324
Bpu10I	CCTNAGC	1 cut(s)	1618
Bpu1102I	GCTNAGC	2 cut(s)	59, 251
Bpu14I	TTCGAA	1 cut(s)	104
BpuEI	CTTGAG	2 cut(s)	374, 1325
BpuMI	CCSGG	2 cut(s)	279, 2679
BsaAI	YACGTR	2 cut(s)	2020, 2880
BsaI	GGTCTC	2 cut(s)	1773, 2018
BsaWI	WCCGGW	3 cut(s)	164, 262, 388
BsaXI	ACNNNNNCTCC	2 cut(s)	1053, 1083
Bse118I	RCCGGY	3 cut(s)	97, 175, 986
Bse1I	ACTGG	5 cut(s)	467, 1392, 1982, 2175, 2485
BseAI	TCCGGA	1 cut(s)	388
BseBI	CCWGG	4 cut(s)	917, 1575, 3122, 3209
BseGI	GGATG	8 cut(s)	421, 1257, 1360, 1819, 2005, 2480, 2674, 3165
BseMII	CTCAG	4 cut(s)	50, 1674, 2085, 3119
BseNI	ACTGG	5 cut(s)	467, 1392, 1982, 2175, 2485
BseRI	GAGGAG	3 cut(s)	419, 587, 1761
BsgI	GTGCAG	1 cut(s)	156
Bsh1285I	CGRYCG	1 cut(s)	1890
BshFI	GGCC	8 cut(s)	69, 1098, 1302, 1465, 1860, 2818, 2991, 3212
BsiEI	CGRYCG	1 cut(s)	1890
BsiHKAI	GWGCWC	2 cut(s)	1539, 2885
BsiHKCI	CYCGRG	1 cut(s)	1138
BsiSI	CCGG	8 cut(s)	98, 165, 176, 263, 278, 389, 987, 2678
BsiWI	CGTACG	1 cut(s)	359
BslFI	GGGAC	2 cut(s)	1325, 3037
BsmAI	GTCTC	4 cut(s)	1203, 1221, 1773, 2018
BsmBI	CGTCTC	1 cut(s)	1221
BsmFI	GGGAC	2 cut(s)	1325, 3037
BsmI	GAATGC	3 cut(s)	258, 546, 1149
BsnI	GGCC	8 cut(s)	69, 1098, 1302, 1465, 1860, 2818, 2991, 3212
Bso31I	GGTCTC	2 cut(s)	1773, 2018
BsoBI	CYCGRG	1 cut(s)	1138
Bsp119I	TTCGAA	1 cut(s)	104
Bsp1286I	GDGCHC	2 cut(s)	1539, 2885
Bsp13I	TCCGGA	1 cut(s)	388
Bsp1407I	TGTACA	1 cut(s)	241
Bsp1720I	GCTNAGC	2 cut(s)	59, 251
Bsp19I	CCATGG	5 cut(s)	964, 1011, 1350, 1710, 3041
BspACI	CCGC	8 cut(s)	179, 255, 288, 320, 956, 1739, 1890, 2248
BspANI	GGCC	8 cut(s)	69, 1098, 1302, 1465, 1860, 2818, 2991, 3212
BspCNI	CTCAG	4 cut(s)	51, 1673, 2086, 3118
BspEI	TCCGGA	1 cut(s)	388
BspLI	GGNNCC	4 cut(s)	1393, 1511, 1624, 3219
BspMAI	CTGCAG	4 cut(s)	682, 768, 841, 2170
BspMI	ACCTGC	3 cut(s)	292, 1883, 2000
BspOI	GCTAGC	1 cut(s)	2415
BspT104I	TTCGAA	1 cut(s)	104
BspTNI	GGTCTC	2 cut(s)	1773, 2018
BsrFI	RCCGGY	3 cut(s)	97, 175, 986
BsrGI	TGTACA	1 cut(s)	241
BsrI	ACTGG	5 cut(s)	467, 1392, 1982, 2175, 2485
BssAI	RCCGGY	3 cut(s)	97, 175, 986
BssNAI	GTATAC	1 cut(s)	2196
BssSI	CACGAG	1 cut(s)	53
BssT1I	CCWWGG	8 cut(s)	964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Bst1107I	GTATAC	1 cut(s)	2196
Bst2BI	CACGAG	1 cut(s)	53
Bst2UI	CCWGG	4 cut(s)	917, 1575, 3122, 3209
Bst4CI	ACNGT	7 cut(s)	1206, 1336, 1342, 1368, 1779, 2155, 3171
Bst6I	CTCTTC	2 cut(s)	387, 3208
BstAPI	GCANNNNNTGC	2 cut(s)	614, 2648
BstAUI	TGTACA	1 cut(s)	241
BstBAI	YACGTR	2 cut(s)	2020, 2880
BstBI	TTCGAA	1 cut(s)	104
BstC8I	GCNNGC	9 cut(s)	424, 1100, 1215, 1497, 1593, 1955, 2253, 2413, 2665
BstDEI	CTNAG	8 cut(s)	59, 251, 1073, 1618, 1660, 2094, 2774, 3105
BstDSI	CCRYGG	6 cut(s)	964, 1011, 1350, 1710, 3041, 3066
BstF5I	GGATG	8 cut(s)	421, 1257, 1360, 1819, 2005, 2480, 2674, 3165
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	2 cut(s)	40, 79
BstMAI	GTCTC	4 cut(s)	1203, 1221, 1773, 2018
BstMCI	CGRYCG	1 cut(s)	1890
BstMWI	GCNNNNNNNGC	8 cut(s)	176, 179, 614, 677, 1462, 1963, 2648, 2889
BstNI	CCWGG	4 cut(s)	917, 1575, 3122, 3209
BstNSI	RCATGY	6 cut(s)	242, 797, 1217, 1503, 2259, 2667
BstSCI	CCNGG	6 cut(s)	277, 915, 1573, 2677, 3120, 3207
BstSFI	CTRYAG	5 cut(s)	678, 764, 837, 2166, 3249
BstV2I	GAAGAC	2 cut(s)	224, 1369
BstX2I	RGATCY	5 cut(s)	475, 1391, 1509, 1755, 2122
BstXI	CCANNNNNNTGG	1 cut(s)	2073
BstYI	RGATCY	5 cut(s)	475, 1391, 1509, 1755, 2122
BstZ17I	GTATAC	1 cut(s)	2196
BsuI	GTATCC	2 cut(s)	141, 3179
BsuRI	GGCC	8 cut(s)	69, 1098, 1302, 1465, 1860, 2818, 2991, 3212
BtgI	CCRYGG	6 cut(s)	964, 1011, 1350, 1710, 3041, 3066
BtgZI	GCGATG	2 cut(s)	2052, 2162
BtrI	CACGTC	2 cut(s)	1231, 1673
BtsCI	GGATG	8 cut(s)	421, 1257, 1360, 1819, 2005, 2480, 2674, 3165
BtsI	GCAGTG	4 cut(s)	541, 687, 761, 2149
BtsIMutI	CAGTG	8 cut(s)	541, 687, 761, 1338, 1775, 2149, 2240, 3343
BveI	ACCTGC	3 cut(s)	292, 1883, 2000
Cac8I	GCNNGC	9 cut(s)	424, 1100, 1215, 1497, 1593, 1955, 2253, 2413, 2665
CaiI	CAGNNNCTG	1 cut(s)	3345
CfoI	GCGC	2 cut(s)	40, 79
Cfr10I	RCCGGY	3 cut(s)	97, 175, 986
Cfr13I	GGNCC	8 cut(s)	347, 826, 860, 924, 1550, 1622, 2816, 2990
CsiI	ACCWGGT	1 cut(s)	915
Csp6I	GTAC	7 cut(s)	202, 242, 360, 512, 568, 984, 3172
CviQI	GTAC	7 cut(s)	202, 242, 360, 512, 568, 984, 3172
DdeI	CTNAG	8 cut(s)	59, 251, 1073, 1618, 1660, 2094, 2774, 3105
DraI	TTTAAA	1 cut(s)	19
Eam1104I	CTCTTC	2 cut(s)	387, 3208
EarI	CTCTTC	2 cut(s)	387, 3208
Eco130I	CCWWGG	8 cut(s)	964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Eco147I	AGGCCT	1 cut(s)	1860
Eco31I	GGTCTC	2 cut(s)	1773, 2018
Eco47I	GGWCC	6 cut(s)	347, 826, 860, 924, 1550, 1622
Eco47III	AGCGCT	1 cut(s)	39
Eco57I	CTGAAG	3 cut(s)	1470, 1587, 2892
Eco88I	CYCGRG	1 cut(s)	1138
EcoO109I	RGGNCCY	1 cut(s)	1622
EcoRII	CCWGG	4 cut(s)	915, 1573, 3120, 3207
EcoT14I	CCWWGG	8 cut(s)	964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
EcoT22I	ATGCAT	2 cut(s)	1215, 3165
ErhI	CCWWGG	8 cut(s)	964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
Esp3I	CGTCTC	1 cut(s)	1221
FaqI	GGGAC	2 cut(s)	1325, 3037
FauI	CCCGC	1 cut(s)	281
FbaI	TGATCA	1 cut(s)	975
FblI	GTMKAC	3 cut(s)	687, 2195, 2689
FokI	GGATG	8 cut(s)	428, 1244, 1367, 1806, 2012, 2467, 2661, 3172
FspBI	CTAG	7 cut(s)	11, 588, 672, 1224, 1275, 2412, 3149
GlaI	GCGC	2 cut(s)	39, 78
GsuI	CTGGAG	2 cut(s)	1956, 2324
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	8 cut(s)	69, 1098, 1302, 1465, 1860, 2818, 2991, 3212
HapII	CCGG	8 cut(s)	98, 165, 176, 263, 278, 389, 987, 2678
HhaI	GCGC	2 cut(s)	40, 79
Hin6I	GCGC	2 cut(s)	38, 77
HinP1I	GCGC	2 cut(s)	38, 77
HincII	GTYRAC	3 cut(s)	384, 688, 3322
HindII	GTYRAC	3 cut(s)	384, 688, 3322
HindIII	AAGCTT	1 cut(s)	1964
HpaI	GTTAAC	2 cut(s)	384, 3322
HpaII	CCGG	8 cut(s)	98, 165, 176, 263, 278, 389, 987, 2678
HphI	GGTGA	5 cut(s)	316, 2055, 2073, 2410, 2927
Hpy166II	GTNNAC	8 cut(s)	350, 384, 541, 688, 2081, 2196, 2690, 3322
Hpy8I	GTNNAC	8 cut(s)	350, 384, 541, 688, 2081, 2196, 2690, 3322
Hpy99I	CGWCG	1 cut(s)	139
HpyCH4III	ACNGT	7 cut(s)	1206, 1336, 1342, 1368, 1779, 2155, 3171
HpyCH4IV	ACGT	8 cut(s)	122, 137, 362, 1230, 1672, 2019, 2839, 2879
HpyF10VI	GCNNNNNNNGC	8 cut(s)	176, 179, 614, 677, 1462, 1963, 2648, 2889
HpyF3I	CTNAG	8 cut(s)	59, 251, 1073, 1618, 1660, 2094, 2774, 3105
HpySE526I	ACGT	8 cut(s)	122, 137, 362, 1230, 1672, 2019, 2839, 2879
HspAI	GCGC	2 cut(s)	38, 77
Kpn2I	TCCGGA	1 cut(s)	388
Ksp22I	TGATCA	1 cut(s)	975
KspAI	GTTAAC	2 cut(s)	384, 3322
LmnI	GCTCC	8 cut(s)	235, 291, 602, 1222, 1438, 1600, 2888, 3223
LweI	GCATC	6 cut(s)	406, 1465, 2235, 2974, 3150, 3298
MabI	ACCWGGT	1 cut(s)	915
MaeI	CTAG	7 cut(s)	11, 588, 672, 1224, 1275, 2412, 3149
MaeII	ACGT	8 cut(s)	122, 137, 362, 1230, 1672, 2019, 2839, 2879
MfeI	CAATTG	5 cut(s)	1174, 1193, 2355, 2540, 3264
MflI	RGATCY	5 cut(s)	475, 1391, 1509, 1755, 2122
MhlI	GDGCHC	2 cut(s)	1539, 2885
MlyI	GAGTC	3 cut(s)	584, 2315, 2891
MmeI	TCCRAC	5 cut(s)	158, 438, 1244, 1353, 2991
Mph1103I	ATGCAT	2 cut(s)	1215, 3165
MroI	TCCGGA	1 cut(s)	388
MroXI	GAANNNNTTC	1 cut(s)	2554
MseI	TTAA	6 cut(s)	18, 231, 383, 798, 1731, 3321
MslI	CAYNNNNRTG	6 cut(s)	263, 1290, 1418, 2260, 2946, 3071
MspA1I	CMGCKG	2 cut(s)	422, 1739
MspI	CCGG	8 cut(s)	98, 165, 176, 263, 278, 389, 987, 2678
MspR9I	CCNGG	6 cut(s)	279, 917, 1575, 2679, 3122, 3209
MunI	CAATTG	5 cut(s)	1174, 1193, 2355, 2540, 3264
Mva1269I	GAATGC	3 cut(s)	258, 546, 1149
MvaI	CCWGG	4 cut(s)	917, 1575, 3122, 3209
MwoI	GCNNNNNNNGC	8 cut(s)	176, 179, 614, 677, 1462, 1963, 2648, 2889
NciI	CCSGG	2 cut(s)	279, 2679
NcoI	CCATGG	5 cut(s)	964, 1011, 1350, 1710, 3041
NheI	GCTAGC	1 cut(s)	2411
NlaIV	GGNNCC	4 cut(s)	1393, 1511, 1624, 3219
NmuCI	GTSAC	8 cut(s)	647, 779, 1336, 1406, 1420, 1519, 2039, 3001
NsiI	ATGCAT	2 cut(s)	1215, 3165
NspI	RCATGY	6 cut(s)	242, 797, 1217, 1503, 2259, 2667
NspV	TTCGAA	1 cut(s)	104
OliI	CACNNNNGTG	1 cut(s)	3071
PaeI	GCATGC	2 cut(s)	1217, 2667
PaeR7I	CTCGAG	1 cut(s)	1138
PaqCI	CACCTGC	1 cut(s)	292
PceI	AGGCCT	1 cut(s)	1860
PciI	ACATGT	1 cut(s)	793
PcsI	WCGNNNNNNNCGW	2 cut(s)	51, 143
PctI	GAATGC	3 cut(s)	258, 546, 1149
PdmI	GAANNNNTTC	1 cut(s)	2554
Pfl23II	CGTACG	1 cut(s)	359
PflMI	CCANNNNNTGG	2 cut(s)	1442, 1781
PleI	GAGTC	3 cut(s)	583, 2314, 2891
PpsI	GAGTC	3 cut(s)	583, 2314, 2891
Ppu21I	YACGTR	2 cut(s)	2020, 2880
PpuMI	RGGWCCY	1 cut(s)	1622
PscI	ACATGT	1 cut(s)	793
PsiI	TTATAA	3 cut(s)	906, 2615, 2796
Psp5II	RGGWCCY	1 cut(s)	1622
Psp6I	CCWGG	4 cut(s)	915, 1573, 3120, 3207
PspGI	CCWGG	4 cut(s)	915, 1573, 3120, 3207
PspLI	CGTACG	1 cut(s)	359
PspN4I	GGNNCC	4 cut(s)	1393, 1511, 1624, 3219
PspPI	GGNCC	8 cut(s)	347, 826, 860, 924, 1550, 1622, 2816, 2990
PspPPI	RGGWCCY	1 cut(s)	1622
PstI	CTGCAG	4 cut(s)	682, 768, 841, 2170
PstNI	CAGNNNCTG	1 cut(s)	3345
PsuI	RGATCY	5 cut(s)	475, 1391, 1509, 1755, 2122
PvuII	CAGCTG	1 cut(s)	422
RsaI	GTAC	7 cut(s)	203, 243, 361, 513, 569, 985, 3173
RsaNI	GTAC	7 cut(s)	202, 242, 360, 512, 568, 984, 3172
RseI	CAYNNNNRTG	6 cut(s)	263, 1290, 1418, 2260, 2946, 3071
SalI	GTCGAC	1 cut(s)	686
SaqAI	TTAA	6 cut(s)	18, 231, 383, 798, 1731, 3321
Sau96I	GGNCC	8 cut(s)	347, 826, 860, 924, 1550, 1622, 2816, 2990
SchI	GAGTC	3 cut(s)	584, 2315, 2891
ScrFI	CCNGG	6 cut(s)	279, 917, 1575, 2679, 3122, 3209
SduI	GDGCHC	2 cut(s)	1539, 2885
SexAI	ACCWGGT	1 cut(s)	915
SfaNI	GCATC	6 cut(s)	406, 1465, 2235, 2974, 3150, 3298
SfcI	CTRYAG	5 cut(s)	678, 764, 837, 2166, 3249
Sfr274I	CTCGAG	1 cut(s)	1138
SfuI	TTCGAA	1 cut(s)	104
SgrAI	CRCCGGYG	1 cut(s)	175
SinI	GGWCC	6 cut(s)	347, 826, 860, 924, 1550, 1622
SlaI	CTCGAG	1 cut(s)	1138
SmiMI	CAYNNNNRTG	6 cut(s)	263, 1290, 1418, 2260, 2946, 3071
SmlI	CTYRAG	3 cut(s)	353, 1138, 1304
SmoI	CTYRAG	3 cut(s)	353, 1138, 1304
SphI	GCATGC	2 cut(s)	1217, 2667
SseBI	AGGCCT	1 cut(s)	1860
SsiI	CCGC	8 cut(s)	179, 255, 288, 320, 956, 1739, 1890, 2248
SspI	AATATT	1 cut(s)	1134
SspMI	CTAG	7 cut(s)	11, 588, 672, 1224, 1275, 2412, 3149
StuI	AGGCCT	1 cut(s)	1860
StyD4I	CCNGG	6 cut(s)	277, 915, 1573, 2677, 3120, 3207
StyI	CCWWGG	8 cut(s)	964, 1011, 1350, 1626, 1710, 1763, 3041, 3213
TaaI	ACNGT	7 cut(s)	1206, 1336, 1342, 1368, 1779, 2155, 3171
TaiI	ACGT	8 cut(s)	125, 140, 365, 1233, 1675, 2022, 2842, 2882
TaqI	TCGA	9 cut(s)	104, 452, 687, 802, 1139, 1360, 1526, 2288, 2928
TatI	WGTACW	2 cut(s)	241, 511
TauI	GCSGC	4 cut(s)	182, 258, 958, 2251
Tru1I	TTAA	6 cut(s)	18, 231, 383, 798, 1731, 3321
Tru9I	TTAA	6 cut(s)	18, 231, 383, 798, 1731, 3321
TscAI	CASTG	7 cut(s)	548, 687, 768, 1345, 1782, 2149, 2247
TseFI	GTSAC	8 cut(s)	647, 779, 1336, 1406, 1420, 1519, 2039, 3001
Tsp45I	GTSAC	8 cut(s)	647, 779, 1336, 1406, 1420, 1519, 2039, 3001
TspGWI	ACGGA	3 cut(s)	2149, 2820, 3041
TspRI	CASTG	7 cut(s)	548, 687, 768, 1345, 1782, 2149, 2247
Van91I	CCANNNNNTGG	2 cut(s)	1442, 1781
VpaK11BI	GGWCC	6 cut(s)	347, 826, 860, 924, 1550, 1622
XapI	RAATTY	2 cut(s)	2860, 3230
XceI	RCATGY	6 cut(s)	242, 797, 1217, 1503, 2259, 2667
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2945
XhoI	CTCGAG	1 cut(s)	1138
XmiI	GTMKAC	3 cut(s)	687, 2195, 2689
XmnI	GAANNNNTTC	1 cut(s)	2554
XspI	CTAG	7 cut(s)	11, 588, 672, 1224, 1275, 2412, 3149
Zsp2I	ATGCAT	2 cut(s)	1215, 3165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.