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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 627 |
| AarI | CACCTGC | 1 cut(s) | 193 |
| Acc36I | ACCTGC | 1 cut(s) | 193 |
| AccI | GTMKAC | 1 cut(s) | 969 |
| AccII | CGCG | 2 cut(s) | 113, 118 |
| AciI | CCGC | 4 cut(s) | 104, 385, 436, 1165 |
| AclWI | GGATC | 1 cut(s) | 495 |
| AcsI | RAATTY | 5 cut(s) | 325, 823, 902, 1043, 1216 |
| AcuI | CTGAAG | 2 cut(s) | 580, 750 |
| AfaI | GTAC | 6 cut(s) | 125, 422, 506, 604, 1192, 1301 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 306, 442, 628, 629 |
| AgeI | ACCGGT | 1 cut(s) | 1000 |
| AgsI | TTSAA | 8 cut(s) | 211, 514, 536, 548, 640, 812, 874, 1181 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 112 |
| AluBI | AGCT | 4 cut(s) | 287, 562, 949, 955 |
| AluI | AGCT | 4 cut(s) | 287, 562, 949, 955 |
| Alw21I | GWGCWC | 1 cut(s) | 962 |
| Alw26I | GTCTC | 2 cut(s) | 719, 789 |
| AlwI | GGATC | 1 cut(s) | 495 |
| AoxI | GGCC | 4 cut(s) | 8, 40, 297, 1161 |
| ApeKI | GCWGC | 7 cut(s) | 188, 368, 461, 562, 866, 1205, 1283 |
| ApoI | RAATTY | 5 cut(s) | 325, 823, 902, 1043, 1216 |
| AsiGI | ACCGGT | 1 cut(s) | 1000 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 738 |
| AspS9I | GGNCC | 4 cut(s) | 8, 41, 85, 1162 |
| AsuHPI | GGTGA | 2 cut(s) | 644, 669 |
| AvaII | GGWCC | 1 cut(s) | 85 |
| BanII | GRGCYC | 1 cut(s) | 435 |
| Bbv12I | GWGCWC | 1 cut(s) | 962 |
| BbvI | GCAGC | 7 cut(s) | 175, 355, 448, 549, 853, 1217, 1270 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 949, 983 |
| BcoDI | GTCTC | 2 cut(s) | 719, 789 |
| BfmI | CTRYAG | 1 cut(s) | 1080 |
| BfuAI | ACCTGC | 1 cut(s) | 193 |
| BglII | AGATCT | 1 cut(s) | 448 |
| BisI | GCNGC | 7 cut(s) | 189, 369, 462, 563, 867, 1206, 1284 |
| BlpI | GCTNAGC | 1 cut(s) | 956 |
| BlsI | GCNGC | 7 cut(s) | 190, 370, 463, 564, 868, 1207, 1285 |
| BmcAI | AGTACT | 1 cut(s) | 422 |
| Bme18I | GGWCC | 1 cut(s) | 85 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 112 |
| BmgT120I | GGNCC | 4 cut(s) | 8, 41, 85, 1162 |
| BmiI | GGNNCC | 5 cut(s) | 86, 87, 180, 432, 609 |
| BmsI | GCATC | 2 cut(s) | 839, 1084 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 956 |
| BpuEI | CTTGAG | 1 cut(s) | 971 |
| BsaWI | WCCGGW | 1 cut(s) | 1000 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 306, 442, 628, 629 |
| Bse118I | RCCGGY | 1 cut(s) | 1000 |
| Bse1I | ACTGG | 2 cut(s) | 203, 770 |
| BseGI | GGATG | 2 cut(s) | 854, 1099 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 306, 442, 628, 629 |
| BseMII | CTCAG | 5 cut(s) | 408, 471, 869, 947, 954 |
| BseNI | ACTGG | 2 cut(s) | 203, 770 |
| BseRI | GAGGAG | 4 cut(s) | 297, 443, 959, 1082 |
| BseXI | GCAGC | 7 cut(s) | 175, 355, 448, 549, 853, 1217, 1270 |
| BsgI | GTGCAG | 1 cut(s) | 548 |
| Bsh1236I | CGCG | 2 cut(s) | 113, 118 |
| BshFI | GGCC | 4 cut(s) | 10, 42, 299, 1163 |
| BshTI | ACCGGT | 1 cut(s) | 1000 |
| BsiHKAI | GWGCWC | 1 cut(s) | 962 |
| BsiSI | CCGG | 1 cut(s) | 1001 |
| BslFI | GGGAC | 3 cut(s) | 98, 490, 605 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 306, 442, 628, 629 |
| BsmAI | GTCTC | 2 cut(s) | 719, 789 |
| BsmFI | GGGAC | 3 cut(s) | 98, 490, 605 |
| BsnI | GGCC | 4 cut(s) | 10, 42, 299, 1163 |
| Bsp1286I | GDGCHC | 2 cut(s) | 435, 962 |
| Bsp143I | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 956 |
| BspACI | CCGC | 4 cut(s) | 104, 385, 436, 1165 |
| BspANI | GGCC | 4 cut(s) | 10, 42, 299, 1163 |
| BspCNI | CTCAG | 5 cut(s) | 409, 472, 868, 948, 955 |
| BspFNI | CGCG | 2 cut(s) | 113, 118 |
| BspHI | TCATGA | 1 cut(s) | 1315 |
| BspLI | GGNNCC | 5 cut(s) | 86, 87, 180, 432, 609 |
| BspMAI | CTGCAG | 1 cut(s) | 1084 |
| BspMI | ACCTGC | 1 cut(s) | 193 |
| BspPI | GGATC | 1 cut(s) | 495 |
| BsrFI | RCCGGY | 1 cut(s) | 1000 |
| BsrI | ACTGG | 2 cut(s) | 203, 770 |
| BssAI | RCCGGY | 1 cut(s) | 1000 |
| BssMI | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| Bst4CI | ACNGT | 2 cut(s) | 893, 1010 |
| Bst6I | CTCTTC | 4 cut(s) | 267, 543, 599, 936 |
| BstC8I | GCNNGC | 4 cut(s) | 301, 560, 917, 1165 |
| BstDEI | CTNAG | 6 cut(s) | 288, 417, 480, 855, 956, 963 |
| BstF5I | GGATG | 2 cut(s) | 854, 1099 |
| BstFNI | CGCG | 2 cut(s) | 113, 118 |
| BstKTI | GATC | 5 cut(s) | 451, 490, 571, 936, 1087 |
| BstMAI | GTCTC | 2 cut(s) | 719, 789 |
| BstMBI | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 110, 284 |
| BstSFI | CTRYAG | 1 cut(s) | 1080 |
| BstUI | CGCG | 2 cut(s) | 113, 118 |
| BstV1I | GCAGC | 7 cut(s) | 175, 355, 448, 549, 853, 1217, 1270 |
| BstX2I | RGATCY | 1 cut(s) | 448 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 664 |
| BstYI | RGATCY | 1 cut(s) | 448 |
| BsuRI | GGCC | 4 cut(s) | 10, 42, 299, 1163 |
| BtsCI | GGATG | 2 cut(s) | 854, 1099 |
| BtsIMutI | CAGTG | 2 cut(s) | 604, 960 |
| BveI | ACCTGC | 1 cut(s) | 193 |
| Cac8I | GCNNGC | 4 cut(s) | 301, 560, 917, 1165 |
| CciI | TCATGA | 1 cut(s) | 1315 |
| Cfr10I | RCCGGY | 1 cut(s) | 1000 |
| Cfr13I | GGNCC | 4 cut(s) | 8, 41, 85, 1162 |
| CseI | GACGC | 3 cut(s) | 102, 137, 1115 |
| Csp6I | GTAC | 6 cut(s) | 124, 421, 505, 603, 1191, 1300 |
| CspAI | ACCGGT | 1 cut(s) | 1000 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 497, 532 |
| CviAII | CATG | 4 cut(s) | 94, 880, 1102, 1316 |
| CviQI | GTAC | 6 cut(s) | 124, 421, 505, 603, 1191, 1300 |
| DdeI | CTNAG | 6 cut(s) | 288, 417, 480, 855, 956, 963 |
| DpnI | GATC | 5 cut(s) | 450, 489, 570, 935, 1086 |
| DpnII | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| DriI | GACNNNNNGTC | 1 cut(s) | 112 |
| Eam1104I | CTCTTC | 4 cut(s) | 267, 543, 599, 936 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 112 |
| EarI | CTCTTC | 4 cut(s) | 267, 543, 599, 936 |
| EciI | GGCGGA | 1 cut(s) | 119 |
| Eco24I | GRGCYC | 1 cut(s) | 435 |
| Eco47I | GGWCC | 1 cut(s) | 85 |
| Eco57I | CTGAAG | 2 cut(s) | 580, 750 |
| EcoO109I | RGGNCCY | 1 cut(s) | 85 |
| EcoT38I | GRGCYC | 1 cut(s) | 435 |
| FaeI | CATG | 4 cut(s) | 97, 883, 1105, 1319 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 449, 481, 853, 885, 933, 965, 1082, 1114 |
| FaqI | GGGAC | 3 cut(s) | 98, 490, 605 |
| FatI | CATG | 4 cut(s) | 93, 879, 1101, 1315 |
| FauI | CCCGC | 3 cut(s) | 378, 443, 1172 |
| FblI | GTMKAC | 1 cut(s) | 969 |
| Fnu4HI | GCNGC | 7 cut(s) | 189, 369, 462, 563, 867, 1206, 1284 |
| FokI | GGATG | 2 cut(s) | 861, 1106 |
| FriOI | GRGCYC | 1 cut(s) | 435 |
| Fsp4HI | GCNGC | 7 cut(s) | 189, 369, 462, 563, 867, 1206, 1284 |
| GluI | GCNGC | 7 cut(s) | 189, 369, 462, 563, 867, 1206, 1284 |
| HaeIII | GGCC | 4 cut(s) | 10, 42, 299, 1163 |
| HapII | CCGG | 1 cut(s) | 1001 |
| HgaI | GACGC | 3 cut(s) | 102, 137, 1115 |
| Hin1II | CATG | 4 cut(s) | 97, 883, 1105, 1319 |
| HincII | GTYRAC | 2 cut(s) | 970, 1130 |
| HindII | GTYRAC | 2 cut(s) | 970, 1130 |
| HinfI | GANTC | 2 cut(s) | 259, 769 |
| HpaII | CCGG | 1 cut(s) | 1001 |
| HphI | GGTGA | 2 cut(s) | 644, 669 |
| Hpy166II | GTNNAC | 4 cut(s) | 505, 970, 1006, 1130 |
| Hpy188I | TCNGA | 6 cut(s) | 267, 412, 694, 720, 858, 1089 |
| Hpy188III | TCNNGA | 5 cut(s) | 67, 454, 533, 1262, 1316 |
| Hpy8I | GTNNAC | 4 cut(s) | 505, 970, 1006, 1130 |
| Hpy99I | CGWCG | 2 cut(s) | 118, 131 |
| HpyAV | CCTTC | 9 cut(s) | 147, 289, 451, 475, 699, 855, 1084, 1108, 1150 |
| HpyCH4III | ACNGT | 2 cut(s) | 893, 1010 |
| HpyCH4V | TGCA | 6 cut(s) | 231, 565, 852, 1082, 1246, 1286 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 110, 284 |
| HpyF3I | CTNAG | 6 cut(s) | 288, 417, 480, 855, 956, 963 |
| Hsp92II | CATG | 4 cut(s) | 97, 883, 1105, 1319 |
| KflI | GGGWCCC | 1 cut(s) | 85 |
| Kzo9I | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| LmnI | GCTCC | 5 cut(s) | 178, 284, 430, 607, 946 |
| Lsp1109I | GCAGC | 7 cut(s) | 175, 355, 448, 549, 853, 1217, 1270 |
| LweI | GCATC | 2 cut(s) | 839, 1084 |
| MaeIII | GTNAC | 2 cut(s) | 388, 785 |
| MalI | GATC | 5 cut(s) | 450, 489, 570, 935, 1086 |
| MboI | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| MboII | GAAGA | 9 cut(s) | 254, 323, 458, 560, 586, 743, 895, 953, 1165 |
| MfeI | CAATTG | 1 cut(s) | 440 |
| MflI | RGATCY | 1 cut(s) | 448 |
| MhlI | GDGCHC | 2 cut(s) | 435, 962 |
| MlyI | GAGTC | 1 cut(s) | 253 |
| MmeI | TCCRAC | 1 cut(s) | 318 |
| MroXI | GAANNNNTTC | 1 cut(s) | 738 |
| MseI | TTAA | 2 cut(s) | 53, 708 |
| MslI | CAYNNNNRTG | 1 cut(s) | 662 |
| MspI | CCGG | 1 cut(s) | 1001 |
| MunI | CAATTG | 1 cut(s) | 440 |
| MvnI | CGCG | 2 cut(s) | 113, 118 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 110, 284 |
| NdeII | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| NlaIII | CATG | 4 cut(s) | 97, 883, 1105, 1319 |
| NlaIV | GGNNCC | 5 cut(s) | 86, 87, 180, 432, 609 |
| NmuCI | GTSAC | 2 cut(s) | 388, 785 |
| PagI | TCATGA | 1 cut(s) | 1315 |
| PaqCI | CACCTGC | 1 cut(s) | 193 |
| PdmI | GAANNNNTTC | 1 cut(s) | 738 |
| PfeI | GAWTC | 1 cut(s) | 769 |
| PinAI | ACCGGT | 1 cut(s) | 1000 |
| PkrI | GCNGC | 7 cut(s) | 190, 370, 463, 564, 868, 1207, 1285 |
| PleI | GAGTC | 1 cut(s) | 253 |
| PpsI | GAGTC | 1 cut(s) | 253 |
| PpuMI | RGGWCCY | 1 cut(s) | 85 |
| PsiI | TTATAA | 1 cut(s) | 627 |
| Psp5II | RGGWCCY | 1 cut(s) | 85 |
| PspN4I | GGNNCC | 5 cut(s) | 86, 87, 180, 432, 609 |
| PspPI | GGNCC | 4 cut(s) | 8, 41, 85, 1162 |
| PspPPI | RGGWCCY | 1 cut(s) | 85 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1217, 1249 |
| PstI | CTGCAG | 1 cut(s) | 1084 |
| PsuI | RGATCY | 1 cut(s) | 448 |
| RsaI | GTAC | 6 cut(s) | 125, 422, 506, 604, 1192, 1301 |
| RsaNI | GTAC | 6 cut(s) | 124, 421, 505, 603, 1191, 1300 |
| RseI | CAYNNNNRTG | 1 cut(s) | 662 |
| SalI | GTCGAC | 1 cut(s) | 968 |
| SaqAI | TTAA | 2 cut(s) | 53, 708 |
| SatI | GCNGC | 7 cut(s) | 189, 369, 462, 563, 867, 1206, 1284 |
| Sau3AI | GATC | 5 cut(s) | 448, 487, 568, 933, 1084 |
| Sau96I | GGNCC | 4 cut(s) | 8, 41, 85, 1162 |
| ScaI | AGTACT | 1 cut(s) | 422 |
| SchI | GAGTC | 1 cut(s) | 253 |
| SduI | GDGCHC | 2 cut(s) | 435, 962 |
| SfaNI | GCATC | 2 cut(s) | 839, 1084 |
| SfcI | CTRYAG | 1 cut(s) | 1080 |
| SinI | GGWCC | 1 cut(s) | 85 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 662 |
| SmlI | CTYRAG | 1 cut(s) | 950 |
| SmoI | CTYRAG | 1 cut(s) | 950 |
| SsiI | CCGC | 4 cut(s) | 104, 385, 436, 1165 |
| SspI | AATATT | 1 cut(s) | 837 |
| TaaI | ACNGT | 2 cut(s) | 893, 1010 |
| TaqI | TCGA | 6 cut(s) | 245, 329, 359, 380, 820, 969 |
| TatI | WGTACW | 1 cut(s) | 420 |
| TfiI | GAWTC | 1 cut(s) | 769 |
| Tru1I | TTAA | 2 cut(s) | 53, 708 |
| Tru9I | TTAA | 2 cut(s) | 53, 708 |
| TscAI | CASTG | 2 cut(s) | 604, 967 |
| TseFI | GTSAC | 2 cut(s) | 388, 785 |
| TseI | GCWGC | 7 cut(s) | 188, 368, 461, 562, 866, 1205, 1283 |
| Tsp45I | GTSAC | 2 cut(s) | 388, 785 |
| TspDTI | ATGAA | 5 cut(s) | 761, 770, 896, 1047, 1090 |
| TspGWI | ACGGA | 3 cut(s) | 620, 1072, 1208 |
| TspRI | CASTG | 2 cut(s) | 604, 967 |
| VpaK11BI | GGWCC | 1 cut(s) | 85 |
| XapI | RAATTY | 5 cut(s) | 325, 823, 902, 1043, 1216 |
| XmiI | GTMKAC | 1 cut(s) | 969 |
| XmnI | GAANNNNTTC | 1 cut(s) | 738 |
| ZrmI | AGTACT | 1 cut(s) | 422 |