FvH4_3g23770

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000222
Physical Location & Seq
Forward (+)
16845430 .. 16846467
1038 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23770.t1

Sequence Viewer

Length: 471 bp
ATGGCAGAACTCAAGAGCAAGCTCGAAGATAAGGTGGCAATTGTCACGGGTGGAGCCAGTGGCATTGGCGAAGCCACCGCACGCCAGTTTGCATTGCACGGTGCCCGTGCGGTGGTCATCGCCGACGTCCAGGACCAGAAAGGCCAGGACGTCGCCGCTTCGATCGGTCCTCACTGCAGCTACATCCACTGCGACGTTACAGACGAGGATCAAGTGAAGAGCCTGGTCGACTCAACGGTCCAGATGCACGGACGCCTAGACATCATGTTCAGCAACGCCGGAATATTCAGCTCGTCCGAGCAGACCGTGCTGGAGTTTGACTTATTCAACTACGATATGCTCATGGCGGTCAACGTACGTGGGATGGCGGCGTGTGTGAAGCACGCGGCGAGGGCTATGGTGGAAGGTGGAGTTAAGCCGTTAAGGGGAGGGTTATATGCACGGCGAGCGTTTTTGCCAGTACTGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

157

Amino Acids

16.7

Weight (kDa)

5.68

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 11 - 136 9.6e-28 short chain dehydrogenase
KR PF08659 14 - 122 1.2e-09 KR domain
adh_short_C2 PF13561 19 - 137 6.1e-26 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01600 FvH4_3g23770 FvH4_3g23780 FvH4_5g15540 FvH4_5g15550 FvH4_5g15560 FvH4_5g36480 FvH4_5g36490 FvH4_5g36512 FvH4_5g36520 FvH4_5g36540
malus_domestica MD06G1187400.v1.1 MD14G1193800.v1.1 MD15G1376500.v1.1
prunus_persica Prupe.5G029400_v2.0.a1 Prupe.5G185600_v2.0.a1 Prupe.5G185700_v2.0.a1
pyrus_communis pycom06g16640 pycom06g16650 pycom06g16660 pycom06g16670 pycom14g16120 pycom15g33650
rosa_chinensis RchiOBHm_Chr7g0180541 RchiOBHm_Chr7g0180551 RchiOBHm_Chr7g0234561 RchiOBHm_Chr7g0238411 RchiOBHm_Chr7g0238441 RchiOBHm_Chr7g0238501 RchiOBHm_Chr7g0238561 RchiOBHm_Chr7g0238571 RchiOBHm_Chr7g0238601 RchiOBHm_Chr7g0242071 RchiOBHm_Chr7g0242111 RchiOBHm_Chr7g0242121 RchiOBHm_Chr7g0242181 RchiOBHm_Chr7g0242191 RchiOBHm_Chr7g0242201 RchiOBHm_Chr7g0242221 RchiOBHm_Chr7g0242291 RchiOBHm_Chr7g0242301
rosa_laevigata RLG00000000902 RLG00000000905 RLG00000000907 RLG00000000908 RLG00000000912 RLG00000005257 RLG00000005259 RLG00000035265
rosa_multiflora Rmu_co8004978.1_g000001 Rmu_co8126788.1_g000001 Rmu_co8332817.1_g000001 Rmu_sc0000429.1_g000001 Rmu_sc0000429.1_g000005 Rmu_sc0000429.1_g000010 Rmu_sc0000429.1_g000017 Rmu_sc0000429.1_g000022 Rmu_sc0000429.1_g000023 Rmu_sc0000429.1_g000030 Rmu_sc0001371.1_g000029 Rmu_sc0001428.1_g000008 Rmu_sc0001428.1_g000012 Rmu_sc0001428.1_g000023 Rmu_sc0001428.1_g000024 Rmu_sc0001536.1_g000009 Rmu_sc0001536.1_g000015 Rmu_sc0003321.1_g000025 Rmu_sc0003322.1_g000017 Rmu_sc0005690.1_g000011 Rmu_sc0005690.1_g000015 Rmu_sc0007123.1_g000006 Rmu_sc0023798.1_g000003 Rmu_sc0025040.1_g000003
rosa_roxburghii Rroxscaffold_3G00222860 Rroxscaffold_3G00222910 Rroxscaffold_3G00222930 Rroxscaffold_3G00222970 Rroxscaffold_3G00272800 Rroxscaffold_3G00272810
rosa_rugosa Rorug06G0433300 Rorug06G0433400 Rorug06G0433400 Rorug07G0312500 Rorug07G0312900 Rorug07G0313300 Rorug07G0313400 Rorug07G0313700 Rorug07G0314100 Rorug07G0314200 Rorug07G0314700
rosa_samantha Rh5DG534900 Rh6BG486700 Rh6CG490700 Rh7AG034600 Rh7AG035000 Rh7AG467300 Rh7AG467400 Rh7AG467700 Rh7AG467800 Rh7AG468100 Rh7AG468800 Rh7AG468900 Rh7AG469100 Rh7AG469200 Rh7AG469400 Rh7AG469500 Rh7AG469600 Rh7AG470000 Rh7AG470100 Rh7BG034600 Rh7BG034700 Rh7BG034900 Rh7BG438400 Rh7BG438800 Rh7BG439000 Rh7BG439100 Rh7BG439300 Rh7BG439400 Rh7BG439800 Rh7CG036300 Rh7CG036500 Rh7CG199800 Rh7CG485400 Rh7CG485800 Rh7CG486000 Rh7CG486100 Rh7CG486200 Rh7CG486600 Rh7CG486700 Rh7DG034900 Rh7DG035200 Rh7DG453900 Rh7DG454300 Rh7DG454400 Rh7DG454500 Rh7DG454600 Rh7DG455000 Rh7DG455100
rosa_wichuraiana Rw0G009440 Rw0G009460 Rw7G002870 Rw7G038750 Rw7G038810 Rw7G038850 Rw7G038860 Rw7G038870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AatII GACGTC 2 cut(s) 129, 153
AccB1I GGYRCC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 228
AccII CGCG 1 cut(s) 386
AciI CCGC 6 cut(s) 78, 110, 156, 347, 368, 386
AclWI GGATC 1 cut(s) 216
AcyI GRCGYC 3 cut(s) 126, 150, 253
AfaI GTAC 2 cut(s) 357, 462
AfiI CCNNNNNNNGG 2 cut(s) 112, 425
AgsI TTSAA 1 cut(s) 328
AjnI CCWGG 3 cut(s) 129, 144, 222
AluBI AGCT 3 cut(s) 22, 180, 291
AluI AGCT 3 cut(s) 22, 180, 291
AlwI GGATC 1 cut(s) 216
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 1 cut(s) 177
AspS9I GGNCC 3 cut(s) 133, 167, 238
AvaII GGWCC 3 cut(s) 133, 167, 238
BaeGI GKGCMC 1 cut(s) 106
BanI GGYRCC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 189
BccI CCATC 1 cut(s) 358
BceAI ACGGC 2 cut(s) 403, 458
BciT130I CCWGG 3 cut(s) 131, 146, 224
BfaI CTAG 2 cut(s) 257, 469
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 4 cut(s) 156, 178, 369, 387
BlsI GCNGC 4 cut(s) 157, 179, 370, 388
BmcAI AGTACT 1 cut(s) 462
Bme1390I CCNGG 3 cut(s) 131, 146, 224
Bme18I GGWCC 3 cut(s) 133, 167, 238
BmgT120I GGNCC 3 cut(s) 133, 167, 238
BmiI GGNNCC 2 cut(s) 55, 103
BmrFI CCNGG 3 cut(s) 131, 146, 224
BmsI GCATC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 332
BsaAI YACGTR 1 cut(s) 359
BsaHI GRCGYC 3 cut(s) 126, 150, 253
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 425
Bse1I ACTGG 4 cut(s) 57, 85, 458, 468
Bse3DI GCAATG 1 cut(s) 92
BseBI CCWGG 3 cut(s) 131, 146, 224
BseGI GGATG 2 cut(s) 183, 369
BseLI CCNNNNNNNGG 2 cut(s) 112, 425
BseMI GCAATG 1 cut(s) 92
BseNI ACTGG 4 cut(s) 57, 85, 458, 468
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 386
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 144
BshNI GGYRCC 1 cut(s) 101
BsiEI CGRYCG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 279
BsiWI CGTACG 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 112, 425
BsnI GGCC 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 2 cut(s) 162, 208
BspACI CCGC 6 cut(s) 78, 110, 156, 347, 368, 386
BspANI GGCC 1 cut(s) 144
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 2 cut(s) 55, 103
BspMAI CTGCAG 1 cut(s) 179
BspPI GGATC 1 cut(s) 216
BspQI GCTCTTC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 101
BsrDI GCAATG 1 cut(s) 92
BsrI ACTGG 4 cut(s) 57, 85, 458, 468
BssMI GATC 2 cut(s) 162, 208
BssNI GRCGYC 3 cut(s) 126, 150, 253
Bst2UI CCWGG 3 cut(s) 131, 146, 224
Bst4CI ACNGT 3 cut(s) 101, 238, 307
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 3 cut(s) 126, 150, 253
BstAPI GCANNNNNTGC 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 359
BstC8I GCNNGC 4 cut(s) 20, 82, 384, 447
BstF5I GGATG 2 cut(s) 183, 369
BstFNI CGCG 1 cut(s) 386
BstKTI GATC 2 cut(s) 165, 211
BstMBI GATC 2 cut(s) 162, 208
BstMCI CGRYCG 1 cut(s) 165
BstMWI GCNNNNNNNGC 3 cut(s) 307, 392, 446
BstNI CCWGG 3 cut(s) 131, 146, 224
BstSCI CCNGG 3 cut(s) 129, 144, 222
BstSFI CTRYAG 1 cut(s) 175
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 189
BsuRI GGCC 1 cut(s) 144
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 2 cut(s) 183, 369
BtsI GCAGTG 2 cut(s) 172, 187
BtsIMutI CAGTG 3 cut(s) 64, 172, 187
Cac8I GCNNGC 4 cut(s) 20, 82, 384, 447
Cfr13I GGNCC 3 cut(s) 133, 167, 238
CseI GACGC 1 cut(s) 261
Csp6I GTAC 2 cut(s) 356, 461
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 2 cut(s) 265, 343
CviJI RGCY 9 cut(s) 22, 56, 74, 144, 180, 222, 291, 395, 418
CviKI_1 RGCY 9 cut(s) 22, 56, 74, 144, 180, 222, 291, 395, 418
CviQI GTAC 2 cut(s) 356, 461
DpnI GATC 2 cut(s) 164, 210
DpnII GATC 2 cut(s) 162, 208
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Eco47I GGWCC 3 cut(s) 133, 167, 238
EcoRII CCWGG 3 cut(s) 129, 144, 222
FaeI CATG 2 cut(s) 268, 346
FaiI YATR 6 cut(s) 266, 338, 344, 398, 436, 438
FatI CATG 2 cut(s) 264, 342
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 4 cut(s) 156, 178, 369, 387
FokI GGATG 2 cut(s) 170, 376
Fsp4HI GCNGC 4 cut(s) 156, 178, 369, 387
FspBI CTAG 2 cut(s) 257, 469
GluI GCNGC 4 cut(s) 156, 178, 369, 387
GsuI CTGGAG 1 cut(s) 332
HaeIII GGCC 1 cut(s) 144
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 261
Hin1I GRCGYC 3 cut(s) 126, 150, 253
Hin1II CATG 2 cut(s) 268, 346
HincII GTYRAC 2 cut(s) 229, 352
HindII GTYRAC 2 cut(s) 229, 352
HinfI GANTC 1 cut(s) 230
HpaII CCGG 1 cut(s) 279
Hpy166II GTNNAC 2 cut(s) 229, 352
Hpy188I TCNGA 1 cut(s) 298
Hpy188III TCNNGA 2 cut(s) 13, 241
Hpy8I GTNNAC 2 cut(s) 229, 352
Hpy99I CGWCG 3 cut(s) 128, 155, 197
HpyAV CCTTC 1 cut(s) 398
HpyCH4III ACNGT 3 cut(s) 101, 238, 307
HpyCH4IV ACGT 5 cut(s) 126, 150, 195, 354, 358
HpyCH4V TGCA 5 cut(s) 92, 97, 177, 247, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 307, 392, 446
HpySE526I ACGT 5 cut(s) 126, 150, 195, 354, 358
Hsp92I GRCGYC 3 cut(s) 126, 150, 253
Hsp92II CATG 2 cut(s) 268, 346
Kzo9I GATC 2 cut(s) 162, 208
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 1 cut(s) 189
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 257, 469
MaeII ACGT 5 cut(s) 126, 150, 195, 354, 358
MaeIII GTNAC 2 cut(s) 43, 196
MalI GATC 2 cut(s) 164, 210
MboI GATC 2 cut(s) 162, 208
MboII GAAGA 2 cut(s) 38, 229
MfeI CAATTG 1 cut(s) 39
MhlI GDGCHC 1 cut(s) 106
MluCI AATT 1 cut(s) 39
MlyI GAGTC 1 cut(s) 224
MnlI CCTC 4 cut(s) 180, 199, 384, 422
MseI TTAA 2 cut(s) 414, 422
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 3 cut(s) 131, 146, 224
MunI CAATTG 1 cut(s) 39
MvaI CCWGG 3 cut(s) 131, 146, 224
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 3 cut(s) 307, 392, 446
NdeII GATC 2 cut(s) 162, 208
NlaIII CATG 2 cut(s) 268, 346
NlaIV GGNNCC 2 cut(s) 55, 103
NmuCI GTSAC 1 cut(s) 43
PciSI GCTCTTC 1 cut(s) 212
PcsI WCGNNNNNNNCGW 1 cut(s) 201
Pfl23II CGTACG 1 cut(s) 355
PfoI TCCNGGA 1 cut(s) 129
PkrI GCNGC 4 cut(s) 157, 179, 370, 388
Ple19I CGATCG 1 cut(s) 165
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Ppu21I YACGTR 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 129, 144, 222
PspGI CCWGG 3 cut(s) 129, 144, 222
PspLI CGTACG 1 cut(s) 355
PspN4I GGNNCC 2 cut(s) 55, 103
PspPI GGNCC 3 cut(s) 133, 167, 238
PstI CTGCAG 1 cut(s) 179
PvuI CGATCG 1 cut(s) 165
RsaI GTAC 2 cut(s) 357, 462
RsaNI GTAC 2 cut(s) 356, 461
SalI GTCGAC 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 212
SaqAI TTAA 2 cut(s) 414, 422
SatI GCNGC 4 cut(s) 156, 178, 369, 387
Sau3AI GATC 2 cut(s) 162, 208
Sau96I GGNCC 3 cut(s) 133, 167, 238
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 3 cut(s) 131, 146, 224
SduI GDGCHC 1 cut(s) 106
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 1 cut(s) 175
SinI GGWCC 3 cut(s) 133, 167, 238
SmlI CTYRAG 1 cut(s) 11
SmoI CTYRAG 1 cut(s) 11
Sse9I AATT 1 cut(s) 39
SsiI CCGC 6 cut(s) 78, 110, 156, 347, 368, 386
SspI AATATT 1 cut(s) 285
SspMI CTAG 2 cut(s) 257, 469
StyD4I CCNGG 3 cut(s) 129, 144, 222
TaaI ACNGT 3 cut(s) 101, 238, 307
TaiI ACGT 5 cut(s) 129, 153, 198, 357, 361
TaqI TCGA 3 cut(s) 24, 161, 228
TaqII GACCGA 1 cut(s) 155
TasI AATT 1 cut(s) 39
TatI WGTACW 1 cut(s) 460
TauI GCSGC 3 cut(s) 158, 371, 389
Tru1I TTAA 2 cut(s) 414, 422
Tru9I TTAA 2 cut(s) 414, 422
TscAI CASTG 3 cut(s) 64, 179, 194
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 177
Tsp45I GTSAC 1 cut(s) 43
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 3 cut(s) 64, 179, 194
VpaK11BI GGWCC 3 cut(s) 133, 167, 238
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 257, 469
ZraI GACGTC 2 cut(s) 127, 151
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.