Rorug06G0433300

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000222
Physical Location & Seq
Forward (+)
56815356 .. 56817676
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0433300.1

Sequence Viewer

Length: 1215 bp
ATGAGCTTAAAAACCAACATTTGTCACTTATTCTCTTGTATGCTTCCTCTTTGGATCGGAGGAAATTCCAAAAGAATGGGATTCCTCTTTATAGAAACCTTCTCTTCTTCTTCTTCTTCCTCTGGTTCCATAATGTCGTCTTCGAAGCAGCTCTTGATATTTGTTTGTAGTCTTGCTGTGGTTTTAGGGCAGTACCCCAGAATCACAGAGGGTCGGGTGAATATACCACCAGGAAAGTCAATGCCGGCGGTTATAGTGTTCGGTGATTCGATCATGGACACCGGCAACAATAACAATCTTAAGTCTGCTGTTAAATGCAATTTCCCTCCATATGGTGAAAATTTCCCAGGAAAAGTTCCAACCGGTAGATTTGGCAATGGAAAAGTCCCCTCAGACTTCATAGCTCAAGAACTGGGAATCAAAGAGTTTGTGCCGGCATATGCGGATACTAGTCTAAAACCTGAAGATCTCAGCACTGGAGTCTGCTTCGCTTCAGGTGGCACCGGATATGACCCTCAGACCCCCGAATTAGTAGCAGTTTTATCGCTATCGGAGCAGTTGAACATGTTCAAGGAGTATATAGGGAAGCTGAAAGCCAATTTTGGCGCGCAGAGAACAAACTTTATCCTCGCCAACAGTCTCTTTCTGGTGGTCGCCGGTAGCGATGACATTGCCAACACCTATTTCACTATTGGCATCAGAAAGGCAGAATATGACGTCCCTGCTTACACTGACCTTATGGTCAACTCTGCCACTAGTTTCCTTCAGGATTTGTATGCACTTGGGGCTAGAAGGTTCGGTGTTTTCAGTGCACCGCCGATAGGTTGTGTACCTTCACAAAGAACTCTAGGTGGAGGAATACAAAGACAGTGTGCAGAAGAGTACAACGACGCAGCGAAGCTATTCAACTCTAAGCTGTCGTCCAACATGCAGTCCTTGAATACAAAGCTGCCCGACAGTAGAATGGTTTATATCGATGTTTACACCCCTCTACTTGATGTCATTCTTCACCCTGCCAAATACGGGTTTAAAGTGGCGGAGAAAGGTTGCTGTGGCACAGGGGCACTAGAGGTAGCGATATTATGCAACAAATTGTCTCCAACGTGTGAAAATCCGGCGGATCATGTATTTTGGGATAGTTATCACCCTACTGAACAAGCATACAGAGTACTCATTCCTCCAGTTCTCCAAAAATACGTAAACCAATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

404

Amino Acids

44.02

Weight (kDa)

7.45

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 84 - 391 7.9e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01600 FvH4_3g23770 FvH4_3g23780 FvH4_5g15540 FvH4_5g15550 FvH4_5g15560 FvH4_5g36480 FvH4_5g36490 FvH4_5g36512 FvH4_5g36520 FvH4_5g36540
malus_domestica MD06G1187400.v1.1 MD14G1193800.v1.1 MD15G1376500.v1.1
prunus_persica Prupe.5G029400_v2.0.a1 Prupe.5G185600_v2.0.a1 Prupe.5G185700_v2.0.a1
pyrus_communis pycom06g16640 pycom06g16650 pycom06g16660 pycom06g16670 pycom14g16120 pycom15g33650
rosa_chinensis RchiOBHm_Chr7g0180541 RchiOBHm_Chr7g0180551 RchiOBHm_Chr7g0234561 RchiOBHm_Chr7g0238411 RchiOBHm_Chr7g0238441 RchiOBHm_Chr7g0238501 RchiOBHm_Chr7g0238561 RchiOBHm_Chr7g0238571 RchiOBHm_Chr7g0238601 RchiOBHm_Chr7g0242071 RchiOBHm_Chr7g0242111 RchiOBHm_Chr7g0242121 RchiOBHm_Chr7g0242181 RchiOBHm_Chr7g0242191 RchiOBHm_Chr7g0242201 RchiOBHm_Chr7g0242221 RchiOBHm_Chr7g0242291 RchiOBHm_Chr7g0242301
rosa_laevigata RLG00000000902 RLG00000000905 RLG00000000907 RLG00000000908 RLG00000000912 RLG00000005257 RLG00000005259 RLG00000035265
rosa_multiflora Rmu_co8004978.1_g000001 Rmu_co8126788.1_g000001 Rmu_co8332817.1_g000001 Rmu_sc0000429.1_g000001 Rmu_sc0000429.1_g000005 Rmu_sc0000429.1_g000010 Rmu_sc0000429.1_g000017 Rmu_sc0000429.1_g000022 Rmu_sc0000429.1_g000023 Rmu_sc0000429.1_g000030 Rmu_sc0001371.1_g000029 Rmu_sc0001428.1_g000008 Rmu_sc0001428.1_g000012 Rmu_sc0001428.1_g000023 Rmu_sc0001428.1_g000024 Rmu_sc0001536.1_g000009 Rmu_sc0001536.1_g000015 Rmu_sc0003321.1_g000025 Rmu_sc0003322.1_g000017 Rmu_sc0005690.1_g000011 Rmu_sc0005690.1_g000015 Rmu_sc0007123.1_g000006 Rmu_sc0023798.1_g000003 Rmu_sc0025040.1_g000003
rosa_roxburghii Rroxscaffold_3G00222860 Rroxscaffold_3G00222910 Rroxscaffold_3G00222930 Rroxscaffold_3G00222970 Rroxscaffold_3G00272800 Rroxscaffold_3G00272810
rosa_rugosa Rorug06G0433300 Rorug06G0433400 Rorug06G0433400 Rorug07G0312500 Rorug07G0312900 Rorug07G0313300 Rorug07G0313400 Rorug07G0313700 Rorug07G0314100 Rorug07G0314200 Rorug07G0314700
rosa_samantha Rh5DG534900 Rh6BG486700 Rh6CG490700 Rh7AG034600 Rh7AG035000 Rh7AG467300 Rh7AG467400 Rh7AG467700 Rh7AG467800 Rh7AG468100 Rh7AG468800 Rh7AG468900 Rh7AG469100 Rh7AG469200 Rh7AG469400 Rh7AG469500 Rh7AG469600 Rh7AG470000 Rh7AG470100 Rh7BG034600 Rh7BG034700 Rh7BG034900 Rh7BG438400 Rh7BG438800 Rh7BG439000 Rh7BG439100 Rh7BG439300 Rh7BG439400 Rh7BG439800 Rh7CG036300 Rh7CG036500 Rh7CG199800 Rh7CG485400 Rh7CG485800 Rh7CG486000 Rh7CG486100 Rh7CG486200 Rh7CG486600 Rh7CG486700 Rh7DG034900 Rh7DG035200 Rh7DG453900 Rh7DG454300 Rh7DG454400 Rh7DG454500 Rh7DG454600 Rh7DG455000 Rh7DG455100
rosa_wichuraiana Rw0G009440 Rw0G009460 Rw7G002870 Rw7G038750 Rw7G038810 Rw7G038850 Rw7G038860 Rw7G038870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 740
AatII GACGTC 1 cut(s) 720
AccB1I GGYRCC 1 cut(s) 500
AccII CGCG 1 cut(s) 608
AciI CCGC 5 cut(s) 248, 443, 815, 1037, 1118
AclWI GGATC 2 cut(s) 62, 1128
AcsI RAATTY 2 cut(s) 64, 340
AcuI CTGAAG 3 cut(s) 477, 483, 749
AcyI GRCGYC 1 cut(s) 717
AfaI GTAC 4 cut(s) 194, 831, 884, 1170
AfiI CCNNNNNNNGG 3 cut(s) 332, 821, 1023
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 2 cut(s) 564, 1103
AgeI ACCGGT 1 cut(s) 362
AgsI TTSAA 4 cut(s) 562, 571, 907, 940
AhlI ACTAGT 2 cut(s) 449, 755
AjnI CCWGG 2 cut(s) 229, 346
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 7 cut(s) 6, 151, 404, 589, 901, 916, 949
AluI AGCT 7 cut(s) 6, 151, 404, 589, 901, 916, 949
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 2 cut(s) 644, 1101
Alw44I GTGCAC 1 cut(s) 810
AlwI GGATC 2 cut(s) 62, 1128
ApaLI GTGCAC 1 cut(s) 810
ApeKI GCWGC 3 cut(s) 148, 893, 949
ApoI RAATTY 2 cut(s) 64, 340
AsiGI ACCGGT 1 cut(s) 362
Asp700I GAANNNNTTC 2 cut(s) 566, 902
AspLEI GCGC 2 cut(s) 608, 610
AsuHPI GGTGA 5 cut(s) 229, 275, 347, 1001, 1136
AsuII TTCGAA 1 cut(s) 143
BaeGI GKGCMC 2 cut(s) 814, 1066
BanI GGYRCC 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 3 cut(s) 160, 905, 936
BcgI CGANNNNNNTGC 4 cut(s) 525, 559, 653, 687
BciT130I CCWGG 2 cut(s) 231, 348
BciVI GTATCC 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 644, 1101
BcuI ACTAGT 2 cut(s) 449, 755
BfaI CTAG 5 cut(s) 450, 756, 789, 848, 1067
BfrI CTTAAG 1 cut(s) 299
BfuI GTATCC 1 cut(s) 439
BglII AGATCT 1 cut(s) 466
BisI GCNGC 3 cut(s) 149, 894, 950
BlsI GCNGC 3 cut(s) 150, 895, 951
BmcAI AGTACT 1 cut(s) 1170
Bme1390I CCNGG 2 cut(s) 231, 348
BmiI GGNNCC 2 cut(s) 127, 502
BmrFI CCNGG 2 cut(s) 231, 348
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 2 cut(s) 498, 1164
Bpu14I TTCGAA 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 975
BsaAI YACGTR 1 cut(s) 1198
BsaBI GATNNNNATC 1 cut(s) 1140
BsaHI GRCGYC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 346
BsaWI WCCGGW 2 cut(s) 362, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 332, 821, 1023
Bse118I RCCGGY 5 cut(s) 244, 281, 362, 433, 656
Bse1I ACTGG 3 cut(s) 417, 481, 1181
Bse3DI GCAATG 2 cut(s) 382, 669
Bse8I GATNNNNATC 1 cut(s) 1140
BseBI CCWGG 2 cut(s) 231, 348
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 1 cut(s) 346
BseJI GATNNNNATC 1 cut(s) 1140
BseLI CCNNNNNNNGG 3 cut(s) 332, 821, 1023
BseMI GCAATG 2 cut(s) 382, 669
BseMII CTCAG 3 cut(s) 405, 484, 530
BseNI ACTGG 3 cut(s) 417, 481, 1181
BsePI GCGCGC 1 cut(s) 606
BseSI GKGCMC 2 cut(s) 814, 1066
BseXI GCAGC 3 cut(s) 160, 905, 936
BsgI GTGCAG 1 cut(s) 894
Bsh1236I CGCG 1 cut(s) 608
BshNI GGYRCC 1 cut(s) 500
BshTI ACCGGT 1 cut(s) 362
BshVI ATCGAT 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 7 cut(s) 245, 282, 363, 434, 504, 657, 1115
BslFI GGGAC 2 cut(s) 371, 704
BslI CCNNNNNNNGG 3 cut(s) 332, 821, 1023
BsmAI GTCTC 2 cut(s) 644, 1101
BsmFI GGGAC 2 cut(s) 371, 704
Bsp119I TTCGAA 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 814, 1066
Bsp143I GATC 4 cut(s) 54, 270, 466, 1120
BspACI CCGC 5 cut(s) 248, 443, 815, 1037, 1118
BspCNI CTCAG 3 cut(s) 404, 483, 529
BspDI ATCGAT 1 cut(s) 975
BspFNI CGCG 1 cut(s) 608
BspLI GGNNCC 2 cut(s) 127, 502
BspPI GGATC 2 cut(s) 62, 1128
BspT104I TTCGAA 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 500
BspTI CTTAAG 1 cut(s) 299
BsrDI GCAATG 2 cut(s) 382, 669
BsrFI RCCGGY 5 cut(s) 244, 281, 362, 433, 656
BsrI ACTGG 3 cut(s) 417, 481, 1181
BssAI RCCGGY 5 cut(s) 244, 281, 362, 433, 656
BssECI CCNNGG 1 cut(s) 346
BssHII GCGCGC 1 cut(s) 606
BssMI GATC 4 cut(s) 54, 270, 466, 1120
BssNI GRCGYC 1 cut(s) 717
Bst2UI CCWGG 2 cut(s) 231, 348
Bst4CI ACNGT 3 cut(s) 638, 870, 959
Bst6I CTCTTC 2 cut(s) 109, 873
BstACI GRCGYC 1 cut(s) 717
BstAFI CTTAAG 1 cut(s) 299
BstBAI YACGTR 1 cut(s) 1198
BstBI TTCGAA 1 cut(s) 143
BstC8I GCNNGC 3 cut(s) 246, 435, 608
BstDEI CTNAG 4 cut(s) 391, 470, 516, 912
BstFNI CGCG 1 cut(s) 608
BstHHI GCGC 2 cut(s) 608, 610
BstKTI GATC 4 cut(s) 57, 273, 469, 1123
BstMAI GTCTC 2 cut(s) 644, 1101
BstMBI GATC 4 cut(s) 54, 270, 466, 1120
BstMWI GCNNNNNNNGC 2 cut(s) 553, 785
BstNI CCWGG 2 cut(s) 231, 348
BstNSI RCATGY 2 cut(s) 568, 931
BstSCI CCNGG 2 cut(s) 229, 346
BstSLI GKGCMC 2 cut(s) 814, 1066
BstSNI TACGTA 1 cut(s) 1198
BstUI CGCG 1 cut(s) 608
BstV1I GCAGC 3 cut(s) 160, 905, 936
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 76
BstYI RGATCY 1 cut(s) 466
Bsu15I ATCGAT 1 cut(s) 975
BsuI GTATCC 1 cut(s) 439
BsuTUI ATCGAT 1 cut(s) 975
BtgZI GCGATG 1 cut(s) 678
BtsIMutI CAGTG 4 cut(s) 474, 729, 814, 875
Cac8I GCNNGC 3 cut(s) 246, 435, 608
CfoI GCGC 2 cut(s) 608, 610
Cfr10I RCCGGY 5 cut(s) 244, 281, 362, 433, 656
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 1 cut(s) 899
Csp6I GTAC 4 cut(s) 193, 830, 883, 1169
CspAI ACCGGT 1 cut(s) 362
CviAII CATG 4 cut(s) 274, 565, 928, 1124
CviJI RGCY 9 cut(s) 6, 151, 404, 589, 596, 788, 901, 916, 949
CviKI_1 RGCY 9 cut(s) 6, 151, 404, 589, 596, 788, 901, 916, 949
CviQI GTAC 4 cut(s) 193, 830, 883, 1169
DdeI CTNAG 4 cut(s) 391, 470, 516, 912
DpnI GATC 4 cut(s) 56, 272, 468, 1122
DpnII GATC 4 cut(s) 54, 270, 466, 1120
DraI TTTAAA 1 cut(s) 1030
DrdI GACNNNNNNGTC 1 cut(s) 740
DseDI GACNNNNNNGTC 1 cut(s) 740
Eam1104I CTCTTC 2 cut(s) 109, 873
EarI CTCTTC 2 cut(s) 109, 873
EciI GGCGGA 2 cut(s) 1052, 1133
Eco105I TACGTA 1 cut(s) 1198
Eco57I CTGAAG 3 cut(s) 477, 483, 749
EcoRII CCWGG 2 cut(s) 229, 346
FaeI CATG 4 cut(s) 277, 568, 931, 1127
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 2 cut(s) 371, 704
FatI CATG 4 cut(s) 273, 564, 927, 1123
FauNDI CATATG 2 cut(s) 331, 439
Fnu4HI GCNGC 3 cut(s) 149, 894, 950
Fsp4HI GCNGC 3 cut(s) 149, 894, 950
FspBI CTAG 5 cut(s) 450, 756, 789, 848, 1067
GlaI GCGC 2 cut(s) 607, 609
GluI GCNGC 3 cut(s) 149, 894, 950
GsuI CTGGAG 2 cut(s) 498, 1164
HapII CCGG 7 cut(s) 245, 282, 363, 434, 504, 657, 1115
HgaI GACGC 1 cut(s) 899
HhaI GCGC 2 cut(s) 608, 610
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 4 cut(s) 277, 568, 931, 1127
Hin6I GCGC 2 cut(s) 606, 608
HinP1I GCGC 2 cut(s) 606, 608
HincII GTYRAC 1 cut(s) 745
HindII GTYRAC 1 cut(s) 745
HinfI GANTC 5 cut(s) 81, 201, 266, 417, 480
HpaII CCGG 7 cut(s) 245, 282, 363, 434, 504, 657, 1115
HphI GGTGA 5 cut(s) 229, 275, 347, 1001, 1136
Hpy166II GTNNAC 5 cut(s) 745, 812, 830, 982, 1201
Hpy188I TCNGA 6 cut(s) 59, 394, 519, 553, 701, 1214
Hpy188III TCNNGA 3 cut(s) 154, 407, 767
Hpy8I GTNNAC 5 cut(s) 745, 812, 830, 982, 1201
Hpy99I CGWCG 1 cut(s) 893
HpyAV CCTTC 4 cut(s) 109, 773, 786, 843
HpyCH4III ACNGT 3 cut(s) 638, 870, 959
HpyCH4IV ACGT 3 cut(s) 717, 1103, 1197
HpyCH4V TGCA 6 cut(s) 318, 779, 812, 875, 931, 1086
HpyF10VI GCNNNNNNNGC 2 cut(s) 553, 785
HpyF3I CTNAG 4 cut(s) 391, 470, 516, 912
HpySE526I ACGT 3 cut(s) 717, 1103, 1197
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 4 cut(s) 277, 568, 931, 1127
HspAI GCGC 2 cut(s) 606, 608
KroI GCCGGC 2 cut(s) 244, 433
KroNI GCCGGC 2 cut(s) 246, 435
Kzo9I GATC 4 cut(s) 54, 270, 466, 1120
LmnI GCTCC 1 cut(s) 553
Lsp1109I GCAGC 3 cut(s) 160, 905, 936
LweI GCATC 1 cut(s) 705
MaeI CTAG 5 cut(s) 450, 756, 789, 848, 1067
MaeII ACGT 3 cut(s) 717, 1103, 1197
MaeIII GTNAC 1 cut(s) 23
MalI GATC 4 cut(s) 56, 272, 468, 1122
MboI GATC 4 cut(s) 54, 270, 466, 1120
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 2 cut(s) 814, 1066
MluCI AATT 7 cut(s) 64, 319, 340, 527, 598, 1091, 1205
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 3 cut(s) 383, 948, 1124
MroNI GCCGGC 2 cut(s) 244, 433
MroXI GAANNNNTTC 2 cut(s) 566, 902
MseI TTAA 4 cut(s) 8, 300, 312, 1029
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 7 cut(s) 245, 282, 363, 434, 504, 657, 1115
MspR9I CCNGG 2 cut(s) 231, 348
MvaI CCWGG 2 cut(s) 231, 348
MvnI CGCG 1 cut(s) 608
MwoI GCNNNNNNNGC 2 cut(s) 553, 785
NaeI GCCGGC 2 cut(s) 246, 435
NdeI CATATG 2 cut(s) 331, 439
NdeII GATC 4 cut(s) 54, 270, 466, 1120
NgoMIV GCCGGC 2 cut(s) 244, 433
NlaIII CATG 4 cut(s) 277, 568, 931, 1127
NlaIV GGNNCC 2 cut(s) 127, 502
NmuCI GTSAC 1 cut(s) 23
NspI RCATGY 2 cut(s) 568, 931
NspV TTCGAA 1 cut(s) 143
PauI GCGCGC 1 cut(s) 606
PciI ACATGT 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PdiI GCCGGC 2 cut(s) 246, 435
PdmI GAANNNNTTC 2 cut(s) 566, 902
PfeI GAWTC 4 cut(s) 81, 201, 266, 417
PinAI ACCGGT 1 cut(s) 362
PkrI GCNGC 3 cut(s) 150, 895, 951
PleI GAGTC 1 cut(s) 488
PpsI GAGTC 1 cut(s) 488
Ppu21I YACGTR 1 cut(s) 1198
PscI ACATGT 1 cut(s) 564
Psp6I CCWGG 2 cut(s) 229, 346
PspGI CCWGG 2 cut(s) 229, 346
PspN4I GGNNCC 2 cut(s) 127, 502
PsuI RGATCY 1 cut(s) 466
PteI GCGCGC 1 cut(s) 606
RsaI GTAC 4 cut(s) 194, 831, 884, 1170
RsaNI GTAC 4 cut(s) 193, 830, 883, 1169
SaqAI TTAA 4 cut(s) 8, 300, 312, 1029
SatI GCNGC 3 cut(s) 149, 894, 950
Sau3AI GATC 4 cut(s) 54, 270, 466, 1120
ScaI AGTACT 1 cut(s) 1170
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 2 cut(s) 231, 348
SduI GDGCHC 2 cut(s) 814, 1066
SfaNI GCATC 1 cut(s) 705
SfuI TTCGAA 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 299, 405
SmoI CTYRAG 2 cut(s) 299, 405
SnaBI TACGTA 1 cut(s) 1198
SpeI ACTAGT 2 cut(s) 449, 755
Sse9I AATT 7 cut(s) 64, 319, 340, 527, 598, 1091, 1205
SsiI CCGC 5 cut(s) 248, 443, 815, 1037, 1118
SspMI CTAG 5 cut(s) 450, 756, 789, 848, 1067
StyD4I CCNGG 2 cut(s) 229, 346
TaaI ACNGT 3 cut(s) 638, 870, 959
TaiI ACGT 3 cut(s) 720, 1106, 1200
TaqI TCGA 3 cut(s) 143, 269, 975
TasI AATT 7 cut(s) 64, 319, 340, 527, 598, 1091, 1205
TatI WGTACW 2 cut(s) 882, 1168
TfiI GAWTC 4 cut(s) 81, 201, 266, 417
Tru1I TTAA 4 cut(s) 8, 300, 312, 1029
Tru9I TTAA 4 cut(s) 8, 300, 312, 1029
TscAI CASTG 4 cut(s) 481, 736, 814, 875
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 3 cut(s) 148, 893, 949
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 1 cut(s) 388
TspRI CASTG 4 cut(s) 481, 736, 814, 875
Vha464I CTTAAG 1 cut(s) 299
VneI GTGCAC 1 cut(s) 810
XapI RAATTY 2 cut(s) 64, 340
XceI RCATGY 2 cut(s) 568, 931
XmnI GAANNNNTTC 2 cut(s) 566, 902
XspI CTAG 5 cut(s) 450, 756, 789, 848, 1067
ZraI GACGTC 1 cut(s) 718
ZrmI AGTACT 1 cut(s) 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.