FvH4_5g15540

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000222
Physical Location & Seq
Reverse (-)
8767691 .. 8768805
1115 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15540.t1

Sequence Viewer

Length: 771 bp
ATGAGTAACAAATTACAAGGCAAAGTAGCCATAGTCACCGGCGGTGCAAGCGGCATTGGCGAGGCCACGGCCCGCCTCTTCGCCGATAATGGAGCTTCCATGGTAGTAATCGCGGACATCCAAGACAATCTCGGCTGCCAACTAGCCGAGTCCATCGGCAACCACAGATGCAGTTACGTGCATTGTAACGTTGCCGATGAAGGACAAGTGAAGTCCATGGTAGAAGCAACGGTCAATAAATTCGGACATCTCGACATCATGTTCAGCAACGCCGGGATAATGAGCAGCGAAAAGCAGACCGTTCTTGACTTTGACATTACAGCCTTTGACCGTTTGTTCGACGTGAACGTGCGCGGCATGGCGTCGTGCGTGAAGCACGCGGCGCGTGTGATGGTGGAGGGGCGCGTGAGGGGGAGCATAGTGTGCACGGCGAGCGTGGGAGCCAACCACGGGATGTCAAAGGACACGGACTACTGCATGTCGAAGCATGCCGTGTTGGGGCTGATGAGGTCGGCGAGCTTGCAGCTGGGGGTGCATGGGATTAGGGTGAACTGCGTCTCACCGAATGGGATGGCGACGCCGCTGACGTGCAAGGCGAGTGGGATGGAGATGGCGGAGCTTCAGAAAGTATATGAGAAGTTCGCGCGGCTGAGAGGAGTGGCGCTCAAGGCGGAGCATGTGGCGAAGGCGGTGCTGTTTCTTGCGAGTGATGATTCTGAGTTTGTTTCTGGGCATGATTTGGTTGTTGATGGATCCTTTGTTCCCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

257

Amino Acids

27.14

Weight (kDa)

6.49

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 8 - 198 9.5e-46 short chain dehydrogenase
KR PF08659 11 - 172 1.6e-08 KR domain
adh_short_C2 PF13561 16 - 251 6.9e-60 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01600 FvH4_3g23770 FvH4_3g23780 FvH4_5g15540 FvH4_5g15550 FvH4_5g15560 FvH4_5g36480 FvH4_5g36490 FvH4_5g36512 FvH4_5g36520 FvH4_5g36540
malus_domestica MD06G1187400.v1.1 MD14G1193800.v1.1 MD15G1376500.v1.1
prunus_persica Prupe.5G029400_v2.0.a1 Prupe.5G185600_v2.0.a1 Prupe.5G185700_v2.0.a1
pyrus_communis pycom06g16640 pycom06g16650 pycom06g16660 pycom06g16670 pycom14g16120 pycom15g33650
rosa_chinensis RchiOBHm_Chr7g0180541 RchiOBHm_Chr7g0180551 RchiOBHm_Chr7g0234561 RchiOBHm_Chr7g0238411 RchiOBHm_Chr7g0238441 RchiOBHm_Chr7g0238501 RchiOBHm_Chr7g0238561 RchiOBHm_Chr7g0238571 RchiOBHm_Chr7g0238601 RchiOBHm_Chr7g0242071 RchiOBHm_Chr7g0242111 RchiOBHm_Chr7g0242121 RchiOBHm_Chr7g0242181 RchiOBHm_Chr7g0242191 RchiOBHm_Chr7g0242201 RchiOBHm_Chr7g0242221 RchiOBHm_Chr7g0242291 RchiOBHm_Chr7g0242301
rosa_laevigata RLG00000000902 RLG00000000905 RLG00000000907 RLG00000000908 RLG00000000912 RLG00000005257 RLG00000005259 RLG00000035265
rosa_multiflora Rmu_co8004978.1_g000001 Rmu_co8126788.1_g000001 Rmu_co8332817.1_g000001 Rmu_sc0000429.1_g000001 Rmu_sc0000429.1_g000005 Rmu_sc0000429.1_g000010 Rmu_sc0000429.1_g000017 Rmu_sc0000429.1_g000022 Rmu_sc0000429.1_g000023 Rmu_sc0000429.1_g000030 Rmu_sc0001371.1_g000029 Rmu_sc0001428.1_g000008 Rmu_sc0001428.1_g000012 Rmu_sc0001428.1_g000023 Rmu_sc0001428.1_g000024 Rmu_sc0001536.1_g000009 Rmu_sc0001536.1_g000015 Rmu_sc0003321.1_g000025 Rmu_sc0003322.1_g000017 Rmu_sc0005690.1_g000011 Rmu_sc0005690.1_g000015 Rmu_sc0007123.1_g000006 Rmu_sc0023798.1_g000003 Rmu_sc0025040.1_g000003
rosa_roxburghii Rroxscaffold_3G00222860 Rroxscaffold_3G00222910 Rroxscaffold_3G00222930 Rroxscaffold_3G00222970 Rroxscaffold_3G00272800 Rroxscaffold_3G00272810
rosa_rugosa Rorug06G0433300 Rorug06G0433400 Rorug06G0433400 Rorug07G0312500 Rorug07G0312900 Rorug07G0313300 Rorug07G0313400 Rorug07G0313700 Rorug07G0314100 Rorug07G0314200 Rorug07G0314700
rosa_samantha Rh5DG534900 Rh6BG486700 Rh6CG490700 Rh7AG034600 Rh7AG035000 Rh7AG467300 Rh7AG467400 Rh7AG467700 Rh7AG467800 Rh7AG468100 Rh7AG468800 Rh7AG468900 Rh7AG469100 Rh7AG469200 Rh7AG469400 Rh7AG469500 Rh7AG469600 Rh7AG470000 Rh7AG470100 Rh7BG034600 Rh7BG034700 Rh7BG034900 Rh7BG438400 Rh7BG438800 Rh7BG439000 Rh7BG439100 Rh7BG439300 Rh7BG439400 Rh7BG439800 Rh7CG036300 Rh7CG036500 Rh7CG199800 Rh7CG485400 Rh7CG485800 Rh7CG486000 Rh7CG486100 Rh7CG486200 Rh7CG486600 Rh7CG486700 Rh7DG034900 Rh7DG035200 Rh7DG453900 Rh7DG454300 Rh7DG454400 Rh7DG454500 Rh7DG454600 Rh7DG455000 Rh7DG455100
rosa_wichuraiana Rw0G009440 Rw0G009460 Rw7G002870 Rw7G038750 Rw7G038810 Rw7G038850 Rw7G038860 Rw7G038870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 7 cut(s) 113, 354, 380, 385, 405, 644, 646
AclI AACGTT 1 cut(s) 189
AclWI GGATC 2 cut(s) 747, 760
AcsI RAATTY 1 cut(s) 239
AcuI CTGAAG 1 cut(s) 605
AcyI GRCGYC 2 cut(s) 362, 578
AfiI CCNNNNNNNGG 2 cut(s) 450, 498
AjiI CACGTC 2 cut(s) 343, 588
AloI GAACNNNNNNTCC 1 cut(s) 744
AluBI AGCT 4 cut(s) 95, 519, 526, 619
AluI AGCT 4 cut(s) 95, 519, 526, 619
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 1 cut(s) 562
Alw44I GTGCAC 1 cut(s) 424
AlwI GGATC 2 cut(s) 747, 760
AoxI GGCC 2 cut(s) 63, 69
ApaLI GTGCAC 1 cut(s) 424
ApeKI GCWGC 3 cut(s) 135, 285, 523
ApoI RAATTY 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
AspLEI GCGC 5 cut(s) 354, 385, 405, 646, 664
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 3 cut(s) 28, 552, 559
BaeGI GKGCMC 1 cut(s) 428
BamHI GGATCC 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 3 cut(s) 122, 297, 535
BccI CCATC 6 cut(s) 161, 385, 565, 598, 604, 743
BceAI ACGGC 3 cut(s) 84, 444, 476
BcgI CGANNNNNNTGC 2 cut(s) 673, 707
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 1 cut(s) 143
BfoI RGCGCY 1 cut(s) 665
BisI GCNGC 8 cut(s) 52, 136, 286, 355, 381, 524, 581, 647
BlsI GCNGC 8 cut(s) 53, 137, 287, 356, 382, 525, 582, 648
Bme1390I CCNGG 1 cut(s) 274
BmgBI CACGTC 2 cut(s) 343, 588
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 442, 754
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 1 cut(s) 158
BplI GAGNNNNNCTC 2 cut(s) 648, 680
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 274
BsaAI YACGTR 1 cut(s) 178
BsaHI GRCGYC 2 cut(s) 362, 578
BsaJI CCNNGG 4 cut(s) 66, 99, 216, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 450, 498
Bse118I RCCGGY 1 cut(s) 38
BseDI CCNNGG 4 cut(s) 66, 99, 216, 448
BseGI GGATG 4 cut(s) 117, 459, 576, 609
BseLI CCNNNNNNNGG 2 cut(s) 450, 498
BseMII CTCAG 2 cut(s) 641, 708
BseRI GAGGAG 1 cut(s) 669
BseSI GKGCMC 1 cut(s) 428
BseXI GCAGC 3 cut(s) 122, 297, 535
BseYI CCCAGC 1 cut(s) 526
Bsh1236I CGCG 7 cut(s) 113, 354, 380, 385, 405, 644, 646
BshFI GGCC 2 cut(s) 65, 71
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 2 cut(s) 39, 273
BslI CCNNNNNNNGG 2 cut(s) 450, 498
BsmAI GTCTC 1 cut(s) 562
BsmBI CGTCTC 1 cut(s) 562
BsnI GGCC 2 cut(s) 65, 71
Bsp1286I GDGCHC 1 cut(s) 428
Bsp143I GATC 1 cut(s) 752
Bsp19I CCATGG 2 cut(s) 99, 216
BspANI GGCC 2 cut(s) 65, 71
BspCNI CTCAG 2 cut(s) 642, 709
BspFNI CGCG 7 cut(s) 113, 354, 380, 385, 405, 644, 646
BspLI GGNNCC 2 cut(s) 442, 754
BspPI GGATC 2 cut(s) 747, 760
BsrFI RCCGGY 1 cut(s) 38
BssAI RCCGGY 1 cut(s) 38
BssECI CCNNGG 4 cut(s) 66, 99, 216, 448
BssMI GATC 1 cut(s) 752
BssNI GRCGYC 2 cut(s) 362, 578
BssT1I CCWWGG 2 cut(s) 99, 216
Bst4CI ACNGT 3 cut(s) 232, 301, 332
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 2 cut(s) 362, 578
BstAPI GCANNNNNTGC 1 cut(s) 423
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 7 cut(s) 49, 73, 378, 433, 489, 517, 521
BstDEI CTNAG 2 cut(s) 650, 717
BstDSI CCRYGG 4 cut(s) 66, 99, 216, 448
BstF5I GGATG 4 cut(s) 117, 459, 576, 609
BstFNI CGCG 7 cut(s) 113, 354, 380, 385, 405, 644, 646
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 5 cut(s) 354, 385, 405, 646, 664
BstKTI GATC 1 cut(s) 755
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 1 cut(s) 752
BstMWI GCNNNNNNNGC 7 cut(s) 48, 57, 382, 423, 432, 532, 668
BstNSI RCATGY 3 cut(s) 481, 491, 680
BstSCI CCNGG 1 cut(s) 272
BstSLI GKGCMC 1 cut(s) 428
BstUI CGCG 7 cut(s) 113, 354, 380, 385, 405, 644, 646
BstV1I GCAGC 3 cut(s) 122, 297, 535
BstX2I RGATCY 1 cut(s) 752
BstYI RGATCY 1 cut(s) 752
BsuRI GGCC 2 cut(s) 65, 71
BtgI CCRYGG 4 cut(s) 66, 99, 216, 448
BtrI CACGTC 2 cut(s) 343, 588
BtsCI GGATG 4 cut(s) 117, 459, 576, 609
Cac8I GCNNGC 7 cut(s) 49, 73, 378, 433, 489, 517, 521
CfoI GCGC 5 cut(s) 354, 385, 405, 646, 664
Cfr10I RCCGGY 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 70
CseI GACGC 3 cut(s) 351, 544, 586
CspCI CAANNNNNGTGG 2 cut(s) 580, 615
DdeI CTNAG 2 cut(s) 650, 717
DpnI GATC 1 cut(s) 754
DpnII GATC 1 cut(s) 752
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 2 cut(s) 629, 686
Eco130I CCWWGG 2 cut(s) 99, 216
Eco57I CTGAAG 1 cut(s) 605
EcoT14I CCWWGG 2 cut(s) 99, 216
ErhI CCWWGG 2 cut(s) 99, 216
Esp3I CGTCTC 1 cut(s) 562
FauI CCCGC 1 cut(s) 80
Fnu4HI GCNGC 8 cut(s) 52, 136, 286, 355, 381, 524, 581, 647
FokI GGATG 4 cut(s) 104, 466, 583, 616
Fsp4HI GCNGC 8 cut(s) 52, 136, 286, 355, 381, 524, 581, 647
FspBI CTAG 1 cut(s) 143
GlaI GCGC 5 cut(s) 353, 384, 404, 645, 663
GluI GCNGC 8 cut(s) 52, 136, 286, 355, 381, 524, 581, 647
GsaI CCCAGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 2 cut(s) 65, 71
HapII CCGG 2 cut(s) 39, 273
HgaI GACGC 3 cut(s) 351, 544, 586
HhaI GCGC 5 cut(s) 354, 385, 405, 646, 664
Hin1I GRCGYC 2 cut(s) 362, 578
Hin6I GCGC 5 cut(s) 352, 383, 403, 644, 662
HinP1I GCGC 5 cut(s) 352, 383, 403, 644, 662
HinfI GANTC 2 cut(s) 149, 713
HpaII CCGG 2 cut(s) 39, 273
HphI GGTGA 3 cut(s) 28, 552, 559
Hpy166II GTNNAC 3 cut(s) 346, 426, 550
Hpy188I TCNGA 3 cut(s) 245, 624, 718
Hpy188III TCNNGA 2 cut(s) 251, 305
Hpy8I GTNNAC 3 cut(s) 346, 426, 550
Hpy99I CGWCG 3 cut(s) 344, 367, 580
HpyAV CCTTC 2 cut(s) 194, 679
HpyCH4III ACNGT 3 cut(s) 232, 301, 332
HpyCH4IV ACGT 5 cut(s) 177, 189, 342, 348, 587
HpyCH4V TGCA 8 cut(s) 47, 171, 181, 426, 477, 523, 535, 591
HpyF10VI GCNNNNNNNGC 7 cut(s) 48, 57, 382, 423, 432, 532, 668
HpyF3I CTNAG 2 cut(s) 650, 717
HpySE526I ACGT 5 cut(s) 177, 189, 342, 348, 587
Hsp92I GRCGYC 2 cut(s) 362, 578
HspAI GCGC 5 cut(s) 352, 383, 403, 644, 662
Kzo9I GATC 1 cut(s) 752
LmnI GCTCC 5 cut(s) 92, 414, 440, 616, 673
LpnPI CCDG 4 cut(s) 52, 286, 512, 714
Lsp1109I GCAGC 3 cut(s) 122, 297, 535
LweI GCATC 1 cut(s) 158
MaeI CTAG 1 cut(s) 143
MaeII ACGT 5 cut(s) 177, 189, 342, 348, 587
MaeIII GTNAC 4 cut(s) 5, 34, 173, 185
MalI GATC 1 cut(s) 754
MboI GATC 1 cut(s) 752
MboII GAAGA 1 cut(s) 70
MflI RGATCY 1 cut(s) 752
MhlI GDGCHC 1 cut(s) 428
MluCI AATT 2 cut(s) 11, 239
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 6 cut(s) 55, 86, 391, 402, 501, 647
MspA1I CMGCKG 2 cut(s) 526, 583
MspI CCGG 2 cut(s) 39, 273
MspR9I CCNGG 1 cut(s) 274
MvnI CGCG 7 cut(s) 113, 354, 380, 385, 405, 644, 646
MwoI GCNNNNNNNGC 7 cut(s) 48, 57, 382, 423, 432, 532, 668
NciI CCSGG 1 cut(s) 274
NcoI CCATGG 2 cut(s) 99, 216
NdeII GATC 1 cut(s) 752
NlaIV GGNNCC 2 cut(s) 442, 754
NmeAIII GCCGAG 2 cut(s) 111, 172
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 3 cut(s) 481, 491, 680
PaeI GCATGC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 4 cut(s) 249, 345, 584, 593
PfeI GAWTC 1 cut(s) 713
PkrI GCNGC 8 cut(s) 53, 137, 287, 356, 382, 525, 582, 648
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Ppu21I YACGTR 1 cut(s) 178
Psp1406I AACGTT 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 526
PspN4I GGNNCC 2 cut(s) 442, 754
PspPI GGNCC 1 cut(s) 70
PsuI RGATCY 1 cut(s) 752
PvuII CAGCTG 1 cut(s) 526
SatI GCNGC 8 cut(s) 52, 136, 286, 355, 381, 524, 581, 647
Sau3AI GATC 1 cut(s) 752
Sau96I GGNCC 1 cut(s) 70
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 274
SduI GDGCHC 1 cut(s) 428
SfaNI GCATC 1 cut(s) 158
SgrAI CRCCGGYG 1 cut(s) 38
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SphI GCATGC 1 cut(s) 491
Sse9I AATT 2 cut(s) 11, 239
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 272
StyI CCWWGG 2 cut(s) 99, 216
TaaI ACNGT 3 cut(s) 232, 301, 332
TaiI ACGT 5 cut(s) 180, 192, 345, 351, 590
TaqI TCGA 3 cut(s) 252, 339, 482
TasI AATT 2 cut(s) 11, 239
TauI GCSGC 5 cut(s) 54, 357, 383, 583, 649
TfiI GAWTC 1 cut(s) 713
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 3 cut(s) 135, 285, 523
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 213
TspGWI ACGGA 1 cut(s) 482
VneI GTGCAC 1 cut(s) 424
XapI RAATTY 1 cut(s) 239
XceI RCATGY 3 cut(s) 481, 491, 680
XspI CTAG 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.