FvH4_5g15560

short-chain dehydrogenase reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000222
Physical Location & Seq
Reverse (-)
8773782 .. 8775234
1453 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g15560.t1

Sequence Viewer

Length: 789 bp
ATGGCAAACCTCACGCTCACGAAGAAGCTTCAAGACAAGGTGGCAATAGTCACCGGTGGCGCTAGTGGCATCGGTGAAGCCACTGCACGCCTCTTTGCTTTGCATGGTGCGCGCGCAGTAGTGATTGCGGACGTCCAGGACGAGAAGGGCCAGGACGTCGCGGCTTCCATCGGCGCTCAATGCAGCTACATCCGCTGCGACGTGAGCGACGAGGACCAGGTCAAGAGCCTGGTCAAGTCAACAGTCCTGACGCACGGCTGCCTGGACATCATGTTCAGCAACGCCGGAATACTCAGCAAAACCAAGCAGGAGGTTCTGGACCTTGACTTGTCCGACTACGACAGGCTGATGGCGGTCAACGTACGAGGGACGGCAGCGTGTGTGAAGCACGCGGCCAAGGCCATGGTGGAAGGCGGAGTGAGGGGGAGCGTGATATGCACGGCAAGCGTCGGGGCGACTAACGGGACGGAAATCTTCACCGACTACACGATGTCAAAGCACGCCATTTTGGGGCTGGTGAGGTGCGCGAGTATACAGCTCGGGACGCACGGGATACGCGTGAACTGCGTATCTCCGGGGCCGACGATGACGCCGATCATGAACACCTCTAATTTTCAGTTTCAGAGTCAGGAAGAGGTAGATAAGCTGAAGTCGACGCTAGGGTTGAAGGGAGGCAAAGAGTTGATGATTCCGGAGCATGTGGCCAGCGCCGTCGTCTACTTAGCTTCCGAGGAATCTCAGTTCGTGAGTGGCCATAATTTGGTCCTCGACGGTGGATTCAAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

263

Amino Acids

27.61

Weight (kDa)

6.16

Isoelectric Point (pI)

22.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 203 3.2e-45 short chain dehydrogenase
KR PF08659 16 - 173 1.4e-11 KR domain
adh_short_C2 PF13561 21 - 260 2.3e-59 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000222)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01600 FvH4_3g23770 FvH4_3g23780 FvH4_5g15540 FvH4_5g15550 FvH4_5g15560 FvH4_5g36480 FvH4_5g36490 FvH4_5g36512 FvH4_5g36520 FvH4_5g36540
malus_domestica MD06G1187400.v1.1 MD14G1193800.v1.1 MD15G1376500.v1.1
prunus_persica Prupe.5G029400_v2.0.a1 Prupe.5G185600_v2.0.a1 Prupe.5G185700_v2.0.a1
pyrus_communis pycom06g16640 pycom06g16650 pycom06g16660 pycom06g16670 pycom14g16120 pycom15g33650
rosa_chinensis RchiOBHm_Chr7g0180541 RchiOBHm_Chr7g0180551 RchiOBHm_Chr7g0234561 RchiOBHm_Chr7g0238411 RchiOBHm_Chr7g0238441 RchiOBHm_Chr7g0238501 RchiOBHm_Chr7g0238561 RchiOBHm_Chr7g0238571 RchiOBHm_Chr7g0238601 RchiOBHm_Chr7g0242071 RchiOBHm_Chr7g0242111 RchiOBHm_Chr7g0242121 RchiOBHm_Chr7g0242181 RchiOBHm_Chr7g0242191 RchiOBHm_Chr7g0242201 RchiOBHm_Chr7g0242221 RchiOBHm_Chr7g0242291 RchiOBHm_Chr7g0242301
rosa_laevigata RLG00000000902 RLG00000000905 RLG00000000907 RLG00000000908 RLG00000000912 RLG00000005257 RLG00000005259 RLG00000035265
rosa_multiflora Rmu_co8004978.1_g000001 Rmu_co8126788.1_g000001 Rmu_co8332817.1_g000001 Rmu_sc0000429.1_g000001 Rmu_sc0000429.1_g000005 Rmu_sc0000429.1_g000010 Rmu_sc0000429.1_g000017 Rmu_sc0000429.1_g000022 Rmu_sc0000429.1_g000023 Rmu_sc0000429.1_g000030 Rmu_sc0001371.1_g000029 Rmu_sc0001428.1_g000008 Rmu_sc0001428.1_g000012 Rmu_sc0001428.1_g000023 Rmu_sc0001428.1_g000024 Rmu_sc0001536.1_g000009 Rmu_sc0001536.1_g000015 Rmu_sc0003321.1_g000025 Rmu_sc0003322.1_g000017 Rmu_sc0005690.1_g000011 Rmu_sc0005690.1_g000015 Rmu_sc0007123.1_g000006 Rmu_sc0023798.1_g000003 Rmu_sc0025040.1_g000003
rosa_roxburghii Rroxscaffold_3G00222860 Rroxscaffold_3G00222910 Rroxscaffold_3G00222930 Rroxscaffold_3G00222970 Rroxscaffold_3G00272800 Rroxscaffold_3G00272810
rosa_rugosa Rorug06G0433300 Rorug06G0433400 Rorug06G0433400 Rorug07G0312500 Rorug07G0312900 Rorug07G0313300 Rorug07G0313400 Rorug07G0313700 Rorug07G0314100 Rorug07G0314200 Rorug07G0314700
rosa_samantha Rh5DG534900 Rh6BG486700 Rh6CG490700 Rh7AG034600 Rh7AG035000 Rh7AG467300 Rh7AG467400 Rh7AG467700 Rh7AG467800 Rh7AG468100 Rh7AG468800 Rh7AG468900 Rh7AG469100 Rh7AG469200 Rh7AG469400 Rh7AG469500 Rh7AG469600 Rh7AG470000 Rh7AG470100 Rh7BG034600 Rh7BG034700 Rh7BG034900 Rh7BG438400 Rh7BG438800 Rh7BG439000 Rh7BG439100 Rh7BG439300 Rh7BG439400 Rh7BG439800 Rh7CG036300 Rh7CG036500 Rh7CG199800 Rh7CG485400 Rh7CG485800 Rh7CG486000 Rh7CG486100 Rh7CG486200 Rh7CG486600 Rh7CG486700 Rh7DG034900 Rh7DG035200 Rh7DG453900 Rh7DG454300 Rh7DG454400 Rh7DG454500 Rh7DG454600 Rh7DG455000 Rh7DG455100
rosa_wichuraiana Rw0G009440 Rw0G009460 Rw7G002870 Rw7G038750 Rw7G038810 Rw7G038850 Rw7G038860 Rw7G038870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 135, 159
AccB7I CCANNNNNTGG 1 cut(s) 760
AccI GTMKAC 3 cut(s) 532, 653, 717
AccII CGCG 6 cut(s) 112, 114, 161, 392, 527, 558
AccIII TCCGGA 1 cut(s) 691
AciI CCGC 6 cut(s) 128, 161, 193, 353, 392, 414
AcoI YGGCCR 3 cut(s) 393, 702, 751
AcuI CTGAAG 1 cut(s) 668
AcyI GRCGYC 3 cut(s) 132, 156, 590
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 2 cut(s) 510, 760
AflIII ACRYGT 1 cut(s) 556
AgeI ACCGGT 1 cut(s) 53
AgsI TTSAA 3 cut(s) 32, 667, 781
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 5 cut(s) 135, 150, 216, 228, 261
AloI GAACNNNNNNTCC 4 cut(s) 257, 289, 725, 757
AluBI AGCT 5 cut(s) 28, 186, 538, 646, 725
AluI AGCT 5 cut(s) 28, 186, 538, 646, 725
Ama87I CYCGRG 1 cut(s) 539
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 6 cut(s) 148, 393, 399, 578, 702, 751
ApeKI GCWGC 4 cut(s) 183, 195, 258, 374
AsiGI ACCGGT 1 cut(s) 53
AspLEI GCGC 7 cut(s) 62, 112, 114, 116, 176, 527, 710
AspS9I GGNCC 5 cut(s) 148, 214, 319, 578, 763
AsuC2I CCSGG 1 cut(s) 576
AsuHPI GGTGA 4 cut(s) 43, 86, 469, 529
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 3 cut(s) 214, 319, 763
BalI TGGCCA 2 cut(s) 704, 753
BbvI GCAGC 4 cut(s) 182, 195, 245, 386
BccI CCATC 2 cut(s) 176, 343
BceAI ACGGC 4 cut(s) 271, 387, 456, 695
BciT130I CCWGG 5 cut(s) 137, 152, 218, 230, 263
BciVI GTATCC 1 cut(s) 546
BcnI CCSGG 1 cut(s) 576
BfaI CTAG 2 cut(s) 63, 659
BfoI RGCGCY 3 cut(s) 63, 177, 711
BfuI GTATCC 1 cut(s) 546
BisI GCNGC 6 cut(s) 162, 184, 196, 259, 375, 393
BlsI GCNGC 6 cut(s) 163, 185, 197, 260, 376, 394
Bme1390I CCNGG 6 cut(s) 137, 152, 218, 230, 263, 576
Bme18I GGWCC 3 cut(s) 214, 319, 763
BmeT110I CYCGRG 1 cut(s) 539
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 5 cut(s) 148, 214, 319, 578, 763
BmiI GGNNCC 1 cut(s) 579
BmrFI CCNGG 6 cut(s) 137, 152, 218, 230, 263, 576
BmsI GCATC 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 576
BsaHI GRCGYC 3 cut(s) 132, 156, 590
BsaJI CCNNGG 4 cut(s) 396, 402, 575, 729
BsaWI WCCGGW 2 cut(s) 53, 691
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 760
Bse118I RCCGGY 1 cut(s) 53
BseAI TCCGGA 1 cut(s) 691
BseBI CCWGG 5 cut(s) 137, 152, 218, 230, 263
BseDI CCNNGG 4 cut(s) 396, 402, 575, 729
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 510, 760
BseMII CTCAG 2 cut(s) 307, 752
BsePI GCGCGC 2 cut(s) 110, 112
BseXI GCAGC 4 cut(s) 182, 195, 245, 386
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 6 cut(s) 112, 114, 161, 392, 527, 558
BshFI GGCC 6 cut(s) 150, 395, 401, 580, 704, 753
BshTI ACCGGT 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 4 cut(s) 54, 285, 575, 692
BsiWI CGTACG 1 cut(s) 361
BslFI GGGAC 3 cut(s) 382, 478, 556
BslI CCNNNNNNNGG 2 cut(s) 510, 760
BsmFI GGGAC 3 cut(s) 382, 478, 556
BsnI GGCC 6 cut(s) 150, 395, 401, 580, 704, 753
BsoBI CYCGRG 1 cut(s) 539
Bsp13I TCCGGA 1 cut(s) 691
Bsp143I GATC 1 cut(s) 594
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 6 cut(s) 128, 161, 193, 353, 392, 414
BspANI GGCC 6 cut(s) 150, 395, 401, 580, 704, 753
BspCNI CTCAG 2 cut(s) 306, 751
BspEI TCCGGA 1 cut(s) 691
BspFNI CGCG 6 cut(s) 112, 114, 161, 392, 527, 558
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 579
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 4 cut(s) 396, 402, 575, 729
BssHII GCGCGC 2 cut(s) 110, 112
BssMI GATC 1 cut(s) 594
BssNAI GTATAC 1 cut(s) 533
BssNI GRCGYC 3 cut(s) 132, 156, 590
BssT1I CCWWGG 2 cut(s) 396, 402
Bst1107I GTATAC 1 cut(s) 533
Bst2UI CCWGG 5 cut(s) 137, 152, 218, 230, 263
Bst4CI ACNGT 2 cut(s) 244, 773
Bst6I CTCTTC 1 cut(s) 627
BstACI GRCGYC 3 cut(s) 132, 156, 590
BstC8I GCNNGC 7 cut(s) 88, 112, 114, 390, 445, 501, 706
BstDEI CTNAG 3 cut(s) 293, 721, 738
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 6 cut(s) 112, 114, 161, 392, 527, 558
BstH2I RGCGCY 3 cut(s) 63, 177, 711
BstHHI GCGC 7 cut(s) 62, 112, 114, 116, 176, 527, 710
BstKTI GATC 1 cut(s) 597
BstMBI GATC 1 cut(s) 594
BstNI CCWGG 5 cut(s) 137, 152, 218, 230, 263
BstNSI RCATGY 1 cut(s) 701
BstSCI CCNGG 6 cut(s) 135, 150, 216, 228, 261, 574
BstUI CGCG 6 cut(s) 112, 114, 161, 392, 527, 558
BstV1I GCAGC 4 cut(s) 182, 195, 245, 386
BstXI CCANNNNNNTGG 1 cut(s) 403
BstZ17I GTATAC 1 cut(s) 533
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 6 cut(s) 150, 395, 401, 580, 704, 753
BtgI CCRYGG 1 cut(s) 402
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 189
BtsI GCAGTG 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 81
Cac8I GCNNGC 7 cut(s) 88, 112, 114, 390, 445, 501, 706
CciI TCATGA 1 cut(s) 597
CfoI GCGC 7 cut(s) 62, 112, 114, 116, 176, 527, 710
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 5 cut(s) 148, 214, 319, 578, 763
CseI GACGC 5 cut(s) 259, 436, 553, 598, 664
CsiI ACCWGGT 1 cut(s) 216
Csp6I GTAC 1 cut(s) 362
CspAI ACCGGT 1 cut(s) 53
CviAII CATG 5 cut(s) 104, 271, 403, 598, 698
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 3 cut(s) 293, 721, 738
DpnI GATC 1 cut(s) 596
DpnII GATC 1 cut(s) 594
EaeI YGGCCR 3 cut(s) 393, 702, 751
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EciI GGCGGA 1 cut(s) 429
Eco130I CCWWGG 2 cut(s) 396, 402
Eco47I GGWCC 3 cut(s) 214, 319, 763
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 539
EcoRII CCWGG 5 cut(s) 135, 150, 216, 228, 261
EcoT14I CCWWGG 2 cut(s) 396, 402
ErhI CCWWGG 2 cut(s) 396, 402
FaeI CATG 5 cut(s) 107, 274, 406, 601, 701
FaiI YATR 9 cut(s) 105, 272, 404, 436, 533, 599, 699, 756, 787
FaqI GGGAC 3 cut(s) 382, 478, 556
FatI CATG 5 cut(s) 103, 270, 402, 597, 697
FblI GTMKAC 3 cut(s) 532, 653, 717
Fnu4HI GCNGC 6 cut(s) 162, 184, 196, 259, 375, 393
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 6 cut(s) 162, 184, 196, 259, 375, 393
FspBI CTAG 2 cut(s) 63, 659
GlaI GCGC 7 cut(s) 61, 111, 113, 115, 175, 526, 709
GluI GCNGC 6 cut(s) 162, 184, 196, 259, 375, 393
HaeII RGCGCY 3 cut(s) 63, 177, 711
HaeIII GGCC 6 cut(s) 150, 395, 401, 580, 704, 753
HapII CCGG 4 cut(s) 54, 285, 575, 692
HgaI GACGC 5 cut(s) 259, 436, 553, 598, 664
HhaI GCGC 7 cut(s) 62, 112, 114, 116, 176, 527, 710
Hin1I GRCGYC 3 cut(s) 132, 156, 590
Hin1II CATG 5 cut(s) 107, 274, 406, 601, 701
Hin6I GCGC 7 cut(s) 60, 110, 112, 114, 174, 525, 708
HinP1I GCGC 7 cut(s) 60, 110, 112, 114, 174, 525, 708
HincII GTYRAC 3 cut(s) 240, 358, 654
HindII GTYRAC 3 cut(s) 240, 358, 654
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 4 cut(s) 625, 688, 734, 777
HpaII CCGG 4 cut(s) 54, 285, 575, 692
HphI GGTGA 4 cut(s) 43, 86, 469, 529
Hpy166II GTNNAC 6 cut(s) 240, 358, 533, 562, 654, 718
Hpy188I TCNGA 3 cut(s) 334, 624, 730
Hpy8I GTNNAC 6 cut(s) 240, 358, 533, 562, 654, 718
Hpy99I CGWCG 8 cut(s) 161, 203, 212, 452, 586, 658, 716, 773
HpyAV CCTTC 3 cut(s) 139, 404, 661
HpyCH4III ACNGT 2 cut(s) 244, 773
HpyCH4IV ACGT 4 cut(s) 132, 156, 201, 360
HpyCH4V TGCA 4 cut(s) 86, 103, 183, 438
HpyF3I CTNAG 3 cut(s) 293, 721, 738
HpySE526I ACGT 4 cut(s) 132, 156, 201, 360
Hsp92I GRCGYC 3 cut(s) 132, 156, 590
Hsp92II CATG 5 cut(s) 107, 274, 406, 601, 701
HspAI GCGC 7 cut(s) 60, 110, 112, 114, 174, 525, 708
Kpn2I TCCGGA 1 cut(s) 691
Kzo9I GATC 1 cut(s) 594
LmnI GCTCC 2 cut(s) 426, 694
Lsp1109I GCAGC 4 cut(s) 182, 195, 245, 386
LweI GCATC 1 cut(s) 78
MabI ACCWGGT 1 cut(s) 216
MaeI CTAG 2 cut(s) 63, 659
MaeII ACGT 4 cut(s) 132, 156, 201, 360
MaeIII GTNAC 1 cut(s) 49
MalI GATC 1 cut(s) 596
MboI GATC 1 cut(s) 594
MboII GAAGA 3 cut(s) 34, 466, 644
MlsI TGGCCA 2 cut(s) 704, 753
MluCI AATT 2 cut(s) 610, 757
MluI ACGCGT 1 cut(s) 556
MluNI TGGCCA 2 cut(s) 704, 753
MlyI GAGTC 1 cut(s) 634
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 2 cut(s) 704, 753
MroI TCCGGA 1 cut(s) 691
MscI TGGCCA 2 cut(s) 704, 753
Msp20I TGGCCA 2 cut(s) 704, 753
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 4 cut(s) 54, 285, 575, 692
MspR9I CCNGG 6 cut(s) 137, 152, 218, 230, 263, 576
MvaI CCWGG 5 cut(s) 137, 152, 218, 230, 263
MvnI CGCG 6 cut(s) 112, 114, 161, 392, 527, 558
NciI CCSGG 1 cut(s) 576
NcoI CCATGG 1 cut(s) 402
NdeII GATC 1 cut(s) 594
NlaIII CATG 5 cut(s) 107, 274, 406, 601, 701
NlaIV GGNNCC 1 cut(s) 579
NmuCI GTSAC 1 cut(s) 49
NspI RCATGY 1 cut(s) 701
PagI TCATGA 1 cut(s) 597
PauI GCGCGC 2 cut(s) 110, 112
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 207, 555, 590
PfeI GAWTC 3 cut(s) 688, 734, 777
Pfl23II CGTACG 1 cut(s) 361
PflFI GACNNNGTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 760
PfoI TCCNGGA 1 cut(s) 135
PinAI ACCGGT 1 cut(s) 53
PkrI GCNGC 6 cut(s) 163, 185, 197, 260, 376, 394
PleI GAGTC 1 cut(s) 633
PpsI GAGTC 1 cut(s) 633
Psp6I CCWGG 5 cut(s) 135, 150, 216, 228, 261
PspGI CCWGG 5 cut(s) 135, 150, 216, 228, 261
PspLI CGTACG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 579
PspPI GGNCC 5 cut(s) 148, 214, 319, 578, 763
PsyI GACNNNGTC 1 cut(s) 218
PteI GCGCGC 2 cut(s) 110, 112
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SalI GTCGAC 1 cut(s) 652
SatI GCNGC 6 cut(s) 162, 184, 196, 259, 375, 393
Sau3AI GATC 1 cut(s) 594
Sau96I GGNCC 5 cut(s) 148, 214, 319, 578, 763
SchI GAGTC 1 cut(s) 634
ScrFI CCNGG 6 cut(s) 137, 152, 218, 230, 263, 576
SexAI ACCWGGT 1 cut(s) 216
SfaNI GCATC 1 cut(s) 78
SgrAI CRCCGGYG 1 cut(s) 53
SinI GGWCC 3 cut(s) 214, 319, 763
Sse9I AATT 2 cut(s) 610, 757
SsiI CCGC 6 cut(s) 128, 161, 193, 353, 392, 414
SspMI CTAG 2 cut(s) 63, 659
StyD4I CCNGG 6 cut(s) 135, 150, 216, 228, 261, 574
StyI CCWWGG 2 cut(s) 396, 402
TaaI ACNGT 2 cut(s) 244, 773
TaiI ACGT 4 cut(s) 135, 159, 204, 363
TaqI TCGA 2 cut(s) 653, 768
TasI AATT 2 cut(s) 610, 757
TauI GCSGC 2 cut(s) 164, 395
TfiI GAWTC 3 cut(s) 688, 734, 777
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 4 cut(s) 183, 195, 258, 374
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 1 cut(s) 614
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 1 cut(s) 88
Tth111I GACNNNGTC 1 cut(s) 218
Van91I CCANNNNNTGG 1 cut(s) 760
VpaK11BI GGWCC 3 cut(s) 214, 319, 763
XceI RCATGY 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 2 cut(s) 403, 511
XmiI GTMKAC 3 cut(s) 532, 653, 717
XspI CTAG 2 cut(s) 63, 659
ZraI GACGTC 2 cut(s) 133, 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.