FvH4_3g42900

emp24/gp25L/p24 family/GOLD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000273
Physical Location & Seq
Forward (+)
35843806 .. 35847204
3399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42900.t1

Sequence Viewer

Length: 2220 bp
ATGGGGAATAAGGAAGGGTATTCACCACTGTTGGAGACCAAGAGAGGAAAGGGAAGAGTGCTATATAGGCTGTTTGCTGCTTCTTTGTTTGCAGCTATATGCTTGGTTTGGGTTTATAGAGCGAGTCACATACCAAAGCCAGGAGAGGATGGAAGGTTTGGCTGGATAGGGCTGTTGGGTGCTGAGTTATGGTTCGGGTTTTATTGGATCCTCACCCAGTCCTGCCGGTGGAATCTTACCTATAGGCAAACTTTCAAGGATAGATTATCTCAAAGATTTGAGAATGAGTTGCCGGGAGTGGACATATTTGTGACCACAGCAGATCCAATCATAGAGCCACCAATGATGGTCATGAACACCGTTCTGTCAGTCATGGCTTATGACTACCCTACGGAGAAGCTCAGCGTGTACTTATCCGATGACGGCGGTTCCGAGCTTACGTACTACGCTCTCTTGGAGGCTGCTGAGTTTGCTAAGCAGTGGATACCGTATTGCAAGAAGTACAGTGTGGAGCCGAGGTCACCATCTGCATATTTTGTCTCGATAGATTCTGACGCAGTTGATCGTGATGATAACAAGGCTAATGACTTGGCTGTAATTAAGAAATCATACAAGGACATGGAGACTAAAGTCGAAAATGCAGTCAAGTTAGGCTACATTTCGGACGAAGTACGATCACAACAGAAAGGGTTTTCTCAATGGGACTCGCATTCGTCTAAACGTGATCACGATACCTATCTTCAAGTTGTAATTGATGGAAGAGACCCAAATGCAATCGATGTCGAAGGAATTGCATTGCCAACTTTGGTGTATTTAGCTCGCGAGAAGAGACCACAGTATCACCATAACTACAAAGCTGGCTCTATGAATGCATTGATTAGGGTTTCTTCAAATATTAGCAACGGGAAGGTCATTCTAAATGTAGACTGTGACATGTATTCGAACAATTCCATGGCCATAAGAGATGCACTTTGCTGCTTAATGGATGAAGAAAAAGGCCATGAGGTTGCATTCGTACAGTTCCCACAAAAATTTGAAAATCTTACCAAGAATGAACTATATATTGCATTCATGCGGACAATCAGCGAGGTGGAAATGCATGCTTTGGATGGTTTTACTGGCCCATTTTACGTTGGAAGTGGATGCTTCCACAGAAGGGATACTCTTTGTGGAAGGAAGTTCACCAAGGGAAGTAAGAGTGACTTGAAATGGGAGATCAGTAGAAAAAGAGAAGAAGCTACCATTCATGAATTGGAAGAAGATTCGAAAGCTCTTGCAAACTGTTCATATGAGCAAAACACCGAGTGGGGAAAAGAGGTGGGTGTGAAATACGGGTGTGCTATTGAAGACGTGTTCACGGGGTTATCGATCCAATGCCGTGGTTGGAAATCGGTGTACTTGAACCCAACAAGGAAAGCTTTCCTAGGAGTAGCTCCAACCACATTGCTTCAGATGCTAGTGCAGCAAAAGAGATGGTCGGAAGGTGGCCTCCAGATTTTGCTTTCAAAGAACAATCCGGTATTGTCTGATCAGATCAGCTTTGGCCAAAAACTTGGATACGCTTATGTCGGATCTTGGTCTATCAATTTCCTTCCAACCCTATATTACACAGTGATCCCTTCACTCTACCTCCTCAAGGGCATTTCCTTATTCCCTCGGGTTTCAAGTCCATGGATCATACCTTTTGCTTACGTGATTATTGCCAAGTACACTGTGAGCTTGCTGGAGTTTCTATGGGCAGGTGGCTCAGCCTTAGGTTGGTGGAACGACCAAAGAATGTGGCTATACAAAAGATCAAGCTCCTACATATTTTCCTTCATCGACACCATGCTGTACCTTCTGGGATATTCTGATTCAGCATTTGTAATAACAGCCAAGACCTCGGATGAAGATGAGTCGCAACGATACGAGAAAGAGATAATGGAATTCGGAGCATCTTCTCCATTGTTTACAGTATTGGCAACACTTGCAGTACTAAATTTGTATTGCTTCGCTGGTTTTGTGAAGGAAGCAATCAGTCTGGGATCACAGGGCATTGCAGAAGTTTATGACACAATGGCATTGCAAATTCTTCTATGTGGGATTTTGATTCTCATCAACCTGCCTTTGTATCAAGCCCTGTATATAAGAAAGGACAAGGGGAAGTTGCCGAGTTCCATAGCGTTGAAGTCGATGGCAGTTGGTGTCTTTGGTTTAGCCTGTCTCAATTTTTCAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt EMP24_GP25L

Protein Analysis

740

Amino Acids

83.6

Weight (kDa)

6.38

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulose_synt PF03552 100 - 399 1.5e-92 Cellulose synthase
Cellulose_synt PF03552 421 - 683 2.3e-44 Cellulose synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55850
fragaria_vesca FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42770 FvH4_3g42800 FvH4_3g42810 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42900
malus_domestica MD03G1028800.v1.1 MD03G1028900.v1.1 MD03G1029000.v1.1 MD03G1029100.v1.1
prunus_persica Prupe.6G024800_v2.0.a1 Prupe.6G024900_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025100_v2.0.a1 Prupe.6G025100_v2.0.a1
pyrus_communis pycom01g03430 pycom03g02330 pycom03g02340 pycom03g02360 pycom03g02380 pycom03g02400 pycom03g02410 pycom05g13920
rosa_chinensis RchiOBHm_Chr5g0076821 RchiOBHm_Chr5g0076831 RchiOBHm_Chr5g0076851 RchiOBHm_Chr5g0076871 RchiOBHm_Chr5g0076891 RchiOBHm_Chr5g0076901 RchiOBHm_Chr5g0076911 RchiOBHm_Chr5g0076921
rosa_laevigata RLG00000036625 RLG00000036626 RLG00000036627 RLG00000036630 RLG00000036632 RLG00000036634 RLG00000036635
rosa_multiflora Rmu_co8084432.1_g000001 Rmu_co8208886.1_g000001 Rmu_co8325177.1_g000001 Rmu_sc0003799.1_g000001 Rmu_sc0003799.1_g000002 Rmu_sc0003799.1_g000006 Rmu_sc0003799.1_g000009 Rmu_sc0004655.1_g000002 Rmu_sc0004655.1_g000014 Rmu_sc0004655.1_g000015 Rmu_sc0007021.1_g000003 Rmu_sc0007021.1_g000013 Rmu_sc0007021.1_g000014 Rmu_sc0008244.1_g000002 Rmu_sc0013263.1_g000004 Rmu_sc0014402.1_g000001 Rmu_ssc0000184.1_g000003 Rmu_ssc0000184.1_g000004 Rmu_ssc0000184.1_g000005 Rmu_ssc0000184.1_g000006 Rmu_ssc0000184.1_g000014 Rmu_ssc0000184.1_g000021
rosa_roxburghii Rroxscaffold_1G00005210 Rroxscaffold_1G00005220 Rroxscaffold_1G00005250 Rroxscaffold_1G00005280 Rroxscaffold_1G00005300 Rroxscaffold_1G00005310
rosa_rugosa Rorug05G0449200 Rorug05G0449300 Rorug05G0449300 Rorug05G0449300 Rorug05G0449500 Rorug05G0449600
rosa_samantha Rh5AG503600 Rh5AG503700 Rh5AG503900 Rh5AG504000 Rh5AG504100 Rh5BG525000 Rh5BG525200 Rh5BG525300 Rh5BG525500 Rh5BG525700 Rh5BG525900 Rh5BG526000 Rh5CG548600 Rh5CG548800 Rh5CG548900 Rh5CG549100 Rh5CG549300 Rh5CG549500 Rh5DG537300 Rh5DG537400 Rh5DG537500 Rh5DG537700 Rh5DG537900 Rh5DG538000 Rh5DG544100
rosa_wichuraiana Rw0G009920 Rw5G046720 Rw5G046730 Rw5G047200 Rw5G047210 Rw5G047240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1730
Acc36I ACCTGC 2 cut(s) 1730, 2109
AccI GTMKAC 1 cut(s) 924
AccII CGCG 1 cut(s) 822
AciI CCGC 2 cut(s) 426, 1075
AclWI GGATC 8 cut(s) 202, 215, 317, 1363, 1579, 1609, 1682, 2032
AcoI YGGCCR 2 cut(s) 954, 1543
AcsI RAATTY 4 cut(s) 1031, 1925, 1978, 2067
AcuI CTGAAG 1 cut(s) 1433
AdeI CACNNNGTG 1 cut(s) 1305
AfaI GTAC 9 cut(s) 410, 443, 503, 672, 1017, 1397, 1709, 1835, 1974
AfiI CCNNNNNNNGG 5 cut(s) 140, 228, 1156, 1636, 1758
AflIII ACRYGT 2 cut(s) 933, 1350
AjiI CACGTC 1 cut(s) 1351
AjnI CCWGG 1 cut(s) 139
Alw26I GTCTC 6 cut(s) 29, 544, 617, 756, 823, 2207
AlwI GGATC 8 cut(s) 202, 215, 317, 1363, 1579, 1609, 1682, 2032
Ama87I CYCGRG 1 cut(s) 1656
AoxI GGCC 5 cut(s) 954, 997, 1120, 1486, 1543
ApeKI GCWGC 5 cut(s) 77, 92, 461, 975, 1462
ApoI RAATTY 4 cut(s) 1031, 1925, 1978, 2067
ArsI GACNNNNNNTTYG 2 cut(s) 627, 659
Asp700I GAANNNNTTC 1 cut(s) 1418
AspA2I CCTAGG 1 cut(s) 1423
AspS9I GGNCC 1 cut(s) 1121
AsuC2I CCSGG 1 cut(s) 294
AsuHPI GGTGA 5 cut(s) 15, 205, 513, 833, 1174
AsuII TTCGAA 2 cut(s) 941, 1265
AvaI CYCGRG 1 cut(s) 1656
AvrII CCTAGG 1 cut(s) 1423
AxyI CCTNAGG 1 cut(s) 1753
BaeI ACNNNNGTAYC 2 cut(s) 821, 854
BalI TGGCCA 2 cut(s) 956, 1545
BamHI GGATCC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 1353
BbvI GCAGC 5 cut(s) 64, 104, 448, 962, 1474
BccI CCATC 7 cut(s) 143, 340, 532, 749, 1103, 1467, 2167
BceAI ACGGC 2 cut(s) 439, 1362
BcgI CGANNNNNNTGC 2 cut(s) 773, 807
BciT130I CCWGG 1 cut(s) 141
BciVI GTATCC 3 cut(s) 477, 1153, 1550
BclI TGATCA 2 cut(s) 724, 1528
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 6 cut(s) 29, 544, 617, 756, 823, 2207
BfaI CTAG 3 cut(s) 1424, 1457, 2218
BfmI CTRYAG 1 cut(s) 241
BfuAI ACCTGC 2 cut(s) 1730, 2109
BfuI GTATCC 3 cut(s) 477, 1153, 1550
BisI GCNGC 5 cut(s) 78, 93, 462, 976, 1463
BlnI CCTAGG 1 cut(s) 1423
BlpI GCTNAGC 3 cut(s) 401, 474, 1747
BlsI GCNGC 5 cut(s) 79, 94, 463, 977, 1464
BmcAI AGTACT 1 cut(s) 1974
Bme1390I CCNGG 2 cut(s) 141, 294
BmeT110I CYCGRG 1 cut(s) 1656
BmgBI CACGTC 1 cut(s) 1351
BmgT120I GGNCC 1 cut(s) 1121
BmiI GGNNCC 3 cut(s) 209, 430, 513
BmrFI CCNGG 2 cut(s) 141, 294
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 4 cut(s) 955, 1133, 1443, 1943
BmuI ACTGGG 1 cut(s) 211
BoxI GACNNNNGTC 1 cut(s) 629
BpiI GAAGAC 1 cut(s) 1353
BpmI CTGGAG 2 cut(s) 1475, 1745
Bpu1102I GCTNAGC 3 cut(s) 401, 474, 1747
Bpu14I TTCGAA 2 cut(s) 941, 1265
BpuEI CTTGAG 1 cut(s) 1619
BpuMI CCSGG 1 cut(s) 294
Bsa29I ATCGAT 2 cut(s) 777, 1367
BsaAI YACGTR 2 cut(s) 441, 1693
BsaBI GATNNNNATC 2 cut(s) 735, 2093
BsaI GGTCTC 3 cut(s) 29, 756, 823
BsaJI CCNNGG 8 cut(s) 515, 951, 1185, 1378, 1423, 1655, 1670, 1881
BsaWI WCCGGW 1 cut(s) 1516
BsaXI ACNNNNNCTCC 4 cut(s) 503, 533, 1614, 1644
Bsc4I CCNNNNNNNGG 5 cut(s) 140, 228, 1156, 1636, 1758
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 2 cut(s) 217, 1123
Bse21I CCTNAGG 1 cut(s) 1753
Bse3DI GCAATG 4 cut(s) 794, 1442, 2034, 2060
Bse8I GATNNNNATC 2 cut(s) 735, 2093
BseBI CCWGG 1 cut(s) 141
BseCI ATCGAT 2 cut(s) 777, 1367
BseDI CCNNGG 8 cut(s) 515, 951, 1185, 1378, 1423, 1655, 1670, 1881
BseGI GGATG 5 cut(s) 154, 991, 1114, 1148, 1891
BseJI GATNNNNATC 2 cut(s) 735, 2093
BseLI CCNNNNNNNGG 5 cut(s) 140, 228, 1156, 1636, 1758
BseMI GCAATG 4 cut(s) 794, 1442, 2034, 2060
BseMII CTCAG 4 cut(s) 174, 415, 456, 1761
BseNI ACTGG 2 cut(s) 217, 1123
BseRI GAGGAG 1 cut(s) 1622
BseXI GCAGC 5 cut(s) 64, 104, 448, 962, 1474
BsgI GTGCAG 1 cut(s) 1481
Bsh1236I CGCG 1 cut(s) 822
BshFI GGCC 5 cut(s) 956, 999, 1122, 1488, 1545
BshVI ATCGAT 2 cut(s) 777, 1367
BsiHKCI CYCGRG 1 cut(s) 1656
BsiSI CCGG 3 cut(s) 226, 293, 1517
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 5 cut(s) 140, 228, 1156, 1636, 1758
BsmAI GTCTC 6 cut(s) 29, 544, 617, 756, 823, 2207
BsmFI GGGAC 1 cut(s) 716
BsmI GAATGC 4 cut(s) 709, 874, 1010, 1067
BsnI GGCC 5 cut(s) 956, 999, 1122, 1488, 1545
Bso31I GGTCTC 3 cut(s) 29, 756, 823
BsoBI CYCGRG 1 cut(s) 1656
Bsp119I TTCGAA 2 cut(s) 941, 1265
Bsp1720I GCTNAGC 3 cut(s) 401, 474, 1747
Bsp19I CCATGG 2 cut(s) 951, 1670
Bsp68I TCGCGA 1 cut(s) 822
BspACI CCGC 2 cut(s) 426, 1075
BspANI GGCC 5 cut(s) 956, 999, 1122, 1488, 1545
BspCNI CTCAG 4 cut(s) 175, 414, 457, 1760
BspDI ATCGAT 2 cut(s) 777, 1367
BspFNI CGCG 1 cut(s) 822
BspHI TCATGA 2 cut(s) 351, 1246
BspLI GGNNCC 3 cut(s) 209, 430, 513
BspMI ACCTGC 2 cut(s) 1730, 2109
BspPI GGATC 8 cut(s) 202, 215, 317, 1363, 1579, 1609, 1682, 2032
BspT104I TTCGAA 2 cut(s) 941, 1265
BspTNI GGTCTC 3 cut(s) 29, 756, 823
BsrDI GCAATG 4 cut(s) 794, 1442, 2034, 2060
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 2 cut(s) 217, 1123
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 8 cut(s) 515, 951, 1185, 1378, 1423, 1655, 1670, 1881
BssT1I CCWWGG 4 cut(s) 951, 1185, 1423, 1670
Bst2UI CCWGG 1 cut(s) 141
Bst6I CTCTTC 3 cut(s) 49, 754, 821
BstAPI GCANNNNNTGC 1 cut(s) 1967
BstBAI YACGTR 2 cut(s) 441, 1693
BstBI TTCGAA 2 cut(s) 941, 1265
BstC8I GCNNGC 4 cut(s) 820, 859, 1101, 1721
BstDEI CTNAG 6 cut(s) 183, 401, 465, 474, 1747, 1753
BstDSI CCRYGG 3 cut(s) 951, 1378, 1670
BstEII GGTNACC 1 cut(s) 519
BstENI CCTNNNNNAGG 1 cut(s) 1634
BstF5I GGATG 5 cut(s) 154, 991, 1114, 1148, 1891
BstFNI CGCG 1 cut(s) 822
BstMAI GTCTC 6 cut(s) 29, 544, 617, 756, 823, 2207
BstMWI GCNNNNNNNGC 5 cut(s) 67, 470, 1453, 1462, 1967
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 2 cut(s) 937, 1103
BstPAI GACNNNNGTC 1 cut(s) 629
BstPI GGTNACC 1 cut(s) 519
BstSCI CCNGG 2 cut(s) 139, 292
BstSFI CTRYAG 1 cut(s) 241
BstSNI TACGTA 1 cut(s) 441
BstUI CGCG 1 cut(s) 822
BstV1I GCAGC 5 cut(s) 64, 104, 448, 962, 1474
BstV2I GAAGAC 1 cut(s) 1353
BstX2I RGATCY 3 cut(s) 207, 322, 1571
BstXI CCANNNNNNTGG 2 cut(s) 1379, 1553
BstYI RGATCY 3 cut(s) 207, 322, 1571
Bsu15I ATCGAT 2 cut(s) 777, 1367
Bsu36I CCTNAGG 1 cut(s) 1753
BsuI GTATCC 3 cut(s) 477, 1153, 1550
BsuRI GGCC 5 cut(s) 956, 999, 1122, 1488, 1545
BsuTUI ATCGAT 2 cut(s) 777, 1367
BtgI CCRYGG 3 cut(s) 951, 1378, 1670
BtrI CACGTC 1 cut(s) 1351
BtsCI GGATG 5 cut(s) 154, 991, 1114, 1148, 1891
BtsI GCAGTG 1 cut(s) 485
BtsIMutI CAGTG 5 cut(s) 26, 485, 511, 1617, 1710
BtuMI TCGCGA 1 cut(s) 822
BveI ACCTGC 2 cut(s) 1730, 2109
Cac8I GCNNGC 4 cut(s) 820, 859, 1101, 1721
CciI TCATGA 2 cut(s) 351, 1246
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 1121
ClaI ATCGAT 2 cut(s) 777, 1367
CseI GACGC 1 cut(s) 563
Csp6I GTAC 9 cut(s) 409, 442, 502, 671, 1016, 1396, 1708, 1834, 1973
CspCI CAANNNNNGTGG 2 cut(s) 1760, 1795
CviQI GTAC 9 cut(s) 409, 442, 502, 671, 1016, 1396, 1708, 1834, 1973
DdeI CTNAG 6 cut(s) 183, 401, 465, 474, 1747, 1753
DraIII CACNNNGTG 1 cut(s) 1305
EaeI YGGCCR 2 cut(s) 954, 1543
Eam1104I CTCTTC 3 cut(s) 49, 754, 821
EarI CTCTTC 3 cut(s) 49, 754, 821
Eco105I TACGTA 1 cut(s) 441
Eco130I CCWWGG 4 cut(s) 951, 1185, 1423, 1670
Eco31I GGTCTC 3 cut(s) 29, 756, 823
Eco57I CTGAAG 1 cut(s) 1433
Eco81I CCTNAGG 1 cut(s) 1753
Eco88I CYCGRG 1 cut(s) 1656
Eco91I GGTNACC 1 cut(s) 519
EcoNI CCTNNNNNAGG 1 cut(s) 1634
EcoO65I GGTNACC 1 cut(s) 519
EcoRI GAATTC 1 cut(s) 1925
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 4 cut(s) 951, 1185, 1423, 1670
EcoT22I ATGCAT 2 cut(s) 874, 1101
ErhI CCWWGG 4 cut(s) 951, 1185, 1423, 1670
FalI AAGNNNNNCTT 4 cut(s) 1187, 1219, 1402, 1434
FaqI GGGAC 1 cut(s) 716
FauNDI CATATG 1 cut(s) 1288
FbaI TGATCA 2 cut(s) 724, 1528
FblI GTMKAC 1 cut(s) 924
Fnu4HI GCNGC 5 cut(s) 78, 93, 462, 976, 1463
FokI GGATG 5 cut(s) 161, 998, 1121, 1155, 1898
Fsp4HI GCNGC 5 cut(s) 78, 93, 462, 976, 1463
FspBI CTAG 3 cut(s) 1424, 1457, 2218
GluI GCNGC 5 cut(s) 78, 93, 462, 976, 1463
GsuI CTGGAG 2 cut(s) 1475, 1745
HaeIII GGCC 5 cut(s) 956, 999, 1122, 1488, 1545
HapII CCGG 3 cut(s) 226, 293, 1517
HgaI GACGC 1 cut(s) 563
HindIII AAGCTT 1 cut(s) 1416
HinfI GANTC 8 cut(s) 124, 232, 548, 704, 1262, 1853, 1895, 2089
HpaII CCGG 3 cut(s) 226, 293, 1517
HphI GGTGA 5 cut(s) 15, 205, 513, 833, 1174
Hpy166II GTNNAC 8 cut(s) 301, 409, 925, 1182, 1356, 1396, 1710, 1950
Hpy188III TCNNGA 7 cut(s) 352, 541, 566, 728, 821, 1247, 1492
Hpy8I GTNNAC 8 cut(s) 301, 409, 925, 1182, 1356, 1396, 1710, 1950
HpyCH4IV ACGT 5 cut(s) 440, 721, 1131, 1350, 1692
HpyF10VI GCNNNNNNNGC 5 cut(s) 67, 470, 1453, 1462, 1967
HpyF3I CTNAG 6 cut(s) 183, 401, 465, 474, 1747, 1753
HpySE526I ACGT 5 cut(s) 440, 721, 1131, 1350, 1692
Ksp22I TGATCA 2 cut(s) 724, 1528
LmnI GCTCC 4 cut(s) 511, 1438, 1805, 1931
Lsp1109I GCAGC 5 cut(s) 64, 104, 448, 962, 1474
LweI GCATC 4 cut(s) 955, 1133, 1443, 1943
MaeI CTAG 3 cut(s) 1424, 1457, 2218
MaeII ACGT 5 cut(s) 440, 721, 1131, 1350, 1692
MaeIII GTNAC 5 cut(s) 125, 310, 519, 929, 1199
MflI RGATCY 3 cut(s) 207, 322, 1571
MlsI TGGCCA 2 cut(s) 956, 1545
MluNI TGGCCA 2 cut(s) 956, 1545
MlyI GAGTC 3 cut(s) 133, 698, 1904
MmeI TCCRAC 7 cut(s) 12, 1114, 1364, 1458, 1460, 1549, 1619
Mox20I TGGCCA 2 cut(s) 956, 1545
Mph1103I ATGCAT 2 cut(s) 874, 1101
MroXI GAANNNNTTC 1 cut(s) 1418
MscI TGGCCA 2 cut(s) 956, 1545
MseI TTAA 2 cut(s) 600, 980
MslI CAYNNNNRTG 1 cut(s) 308
Msp20I TGGCCA 2 cut(s) 956, 1545
MspI CCGG 3 cut(s) 226, 293, 1517
MspR9I CCNGG 2 cut(s) 141, 294
Mva1269I GAATGC 4 cut(s) 709, 874, 1010, 1067
MvaI CCWGG 1 cut(s) 141
MvnI CGCG 1 cut(s) 822
MwoI GCNNNNNNNGC 5 cut(s) 67, 470, 1453, 1462, 1967
NciI CCSGG 1 cut(s) 294
NcoI CCATGG 2 cut(s) 951, 1670
NdeI CATATG 1 cut(s) 1288
NlaIV GGNNCC 3 cut(s) 209, 430, 513
NmeAIII GCCGAG 2 cut(s) 540, 2175
NmuCI GTSAC 5 cut(s) 125, 310, 519, 929, 1199
NruI TCGCGA 1 cut(s) 822
NsiI ATGCAT 2 cut(s) 874, 1101
NspI RCATGY 2 cut(s) 937, 1103
NspV TTCGAA 2 cut(s) 941, 1265
PaeI GCATGC 1 cut(s) 1103
PagI TCATGA 2 cut(s) 351, 1246
PaqCI CACCTGC 1 cut(s) 1730
PciI ACATGT 1 cut(s) 933
PcsI WCGNNNNNNNCGW 2 cut(s) 429, 1364
PctI GAATGC 4 cut(s) 709, 874, 1010, 1067
PdmI GAANNNNTTC 1 cut(s) 1418
PfeI GAWTC 5 cut(s) 232, 548, 1262, 1853, 2089
PkrI GCNGC 5 cut(s) 79, 94, 463, 977, 1464
PleI GAGTC 3 cut(s) 132, 698, 1903
PpsI GAGTC 3 cut(s) 132, 698, 1903
Ppu21I YACGTR 2 cut(s) 441, 1693
PscI ACATGT 1 cut(s) 933
PshAI GACNNNNGTC 1 cut(s) 629
Psp6I CCWGG 1 cut(s) 139
PspEI GGTNACC 1 cut(s) 519
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 3 cut(s) 209, 430, 513
PspPI GGNCC 1 cut(s) 1121
PsuI RGATCY 3 cut(s) 207, 322, 1571
RruI TCGCGA 1 cut(s) 822
RsaI GTAC 9 cut(s) 410, 443, 503, 672, 1017, 1397, 1709, 1835, 1974
RsaNI GTAC 9 cut(s) 409, 442, 502, 671, 1016, 1396, 1708, 1834, 1973
RseI CAYNNNNRTG 1 cut(s) 308
SaqAI TTAA 2 cut(s) 600, 980
SatI GCNGC 5 cut(s) 78, 93, 462, 976, 1463
Sau96I GGNCC 1 cut(s) 1121
ScaI AGTACT 1 cut(s) 1974
SchI GAGTC 3 cut(s) 133, 698, 1904
ScrFI CCNGG 2 cut(s) 141, 294
SfaNI GCATC 4 cut(s) 955, 1133, 1443, 1943
SfcI CTRYAG 1 cut(s) 241
SfuI TTCGAA 2 cut(s) 941, 1265
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 1 cut(s) 1634
SmoI CTYRAG 1 cut(s) 1634
SnaBI TACGTA 1 cut(s) 441
SphI GCATGC 1 cut(s) 1103
SsiI CCGC 2 cut(s) 426, 1075
SspI AATATT 1 cut(s) 895
SspMI CTAG 3 cut(s) 1424, 1457, 2218
StyD4I CCNGG 2 cut(s) 139, 292
StyI CCWWGG 4 cut(s) 951, 1185, 1423, 1670
TaiI ACGT 5 cut(s) 443, 724, 1134, 1353, 1695
TaqI TCGA 9 cut(s) 542, 633, 777, 783, 941, 1265, 1367, 1821, 2171
TatI WGTACW 5 cut(s) 408, 501, 1395, 1707, 1972
TfiI GAWTC 5 cut(s) 232, 548, 1262, 1853, 2089
Tru1I TTAA 2 cut(s) 600, 980
Tru9I TTAA 2 cut(s) 600, 980
TscAI CASTG 5 cut(s) 33, 485, 511, 1617, 1717
TseFI GTSAC 5 cut(s) 125, 310, 519, 929, 1199
TseI GCWGC 5 cut(s) 77, 92, 461, 975, 1462
Tsp45I GTSAC 5 cut(s) 125, 310, 519, 929, 1199
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 5 cut(s) 33, 485, 511, 1617, 1717
XagI CCTNNNNNAGG 1 cut(s) 1634
XapI RAATTY 4 cut(s) 1031, 1925, 1978, 2067
XceI RCATGY 2 cut(s) 937, 1103
XcmI CCANNNNNNNNNTGG 1 cut(s) 1249
XmaJI CCTAGG 1 cut(s) 1423
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 1 cut(s) 1418
XspI CTAG 3 cut(s) 1424, 1457, 2218
ZrmI AGTACT 1 cut(s) 1974
Zsp2I ATGCAT 2 cut(s) 874, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.