Rh5BG525000

Belongs to the glycosyltransferase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000273
Physical Location & Seq
Forward (+)
82796792 .. 82813517
16726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG525000.1

Sequence Viewer

Length: 2997 bp
ATGTCGAAGAAACTTTATGAAGAAATGAAAAATCGGATCGATTCTGTTGTTGAAAGTGGGAAGATACCAGAAGAAACAAGGAATCAACACAAAGGGTTCTCAGAATGGAACCTTAACGTAGCAAAGAATGATCATCACTCAATTGTGCAGATTATAACTGATGGAAGAGACACAAATGCCGTGGACAACGATGGATGCCGATTACCAACTATTGTCTACATGTCAAGAGAAAAGAGACCCCAACAGTCACACAACTTCAAAGCTGGAGCCATGAATGCACTGCTGAGAGTCTCATCAGACATAAGCAATGCACCCTTCATTCTCGACTTGGACTGTGACATGTACGCAAACAATGCAGATGCAATCCGAGAGGCACTTTGTTTCTTCTTGGATGAAAAGACTGGCCACGAGACTGCTTATGTGCAACATCCGCAATTCTACAACAATCTCACAAAGAATGATATCTATGGAAATGCATGCTTCGTACCCCGGGCGGTTGAACTGGCTGGGTTAGATGGATATGGAGCGGCCTTGTACTGTGGCACTGGATGTTTACATCGAAGAGAATGTCTTTGTGGAAAGAAGTATTCGAAGGGTTACACAGAAAAATGGAACATTGATGGCCAGAAGAGTACTATTGACAAAAGTATCCAAGAATTGGAGGAATCTACGAAACCTCTTATCGATTGTAGCTATGAAAAGGGCAGTCAATGGGGAAAAGAGATGGGACTGATATATGGATGCCCTGTCGAAGATATAGTTACTGGTTTGGCAATACAATGCAGGGGTTGGAAATCAGTTTATTACAATCCAGAAAGGCCTTCCTTTGTTGGTGTTGCTCCAAACACCCTAGAAATCGCACTTGTTCAACAAAAGAGGTGGTCTGAGGGCATGTTTCAGATATTTTTCTCCGAGTATTATTGCCCTTTCATATATGGACATGGGAAGATACACTTTGGTGCCCAAATGGGATATTGCCTCTACCTTCTATGGGCTCCAGTTTCATTCCCAACTTTGTATTATGCACTTGTTCCTCCTCTTTGCTTGCTCCATGGCATTCCCTTGTTCCCCAAGGTGTCAAGCCTATGGTTCCTAGCATTTGCTTATGTTTTTGTAGCAAAGAATGCATACAGCATAGTTGAATTTGTGATGTGTGGGGGTACACTCAAAGCATGGTGGAACCTACAGAGAATGTGGCTTATCCGACGAGTCACTTCGTACTTCTTTGCCTTCTTCGACACCATAAAGAGGCAATTGGGGCTATCTGAAACACACTTTGCCCTCACCGATAAGGTTGTCACCGAGGATGTATTAAAGAGGTATGAGCAAGAGGTTTTGGAATTTGGAAGCTCAAGCAATATGTACACTGTAATGGCAACATCGGCATTGCTGAACTTGGTGAGCTTAGTTTTGGGGACAAAGCGGGTTGTGATGGATTTAGAGTTGAAGGCCTTGGACCAGTTCATTAGTCAAGTTATTCTTTGTGGCATATTGGTTTTGATCAATATACCAGTGTATGAAGCTCTTTTCTTGCGCAGTGATAAGGGCCATATACCATCCTCTGTCATGTACAAATATGAGGATGAGTTGCCCGGAGTGGACATATTCGTGTGCACGGCAGACCAAACTATAGAGCCGCCGATGATGGTGATAAACACTGTTTTATCAGTAATGGCTTATGATTACCCACCGGAGAAGCTCAGCGTGTATCTATCCGATGACGGCGGATCCGAACTTACATGTTATGCTCTATTGGAAGCTGCTGAGTTTGCTAAGCATTGGATACCATATTGCAAGAAGTACAATGTGGAGCCAAGGTCACCTGCTGCTTATTTTGCCTCACTAGCTTCTGATAATGCAGGGGATTTCACTCTCATTAAGAAATTATACAAAGACATGGAGAATAAAATCAAAATTGCAGTGAAGTTAGGCAGCATTTCTGAAGAAGTACGATCAAAACACAGAGGCTTTTCTCAATGGGATTCGTATTCATCTCCACGTGACCATGACACCATTCTACAAATAGTAGTTGATGGGAGAGATCCAAATGCAAGAGATGTTGAAGGGTGTGGATTGCCAACCGTAGTGTATTTGGCTCGCGAGAAGAGACCCCAATATCACCATAACTTCAAAGCCGGAGCTATGAATGCACTGATTAGGGTATCTTCAAACATTAGCAATGGGAAGGTTATTCTTAATGTAGACTGTGATATGTATTCAAACAACTCTAAGGCCATAAGGGATGCACTCTGCTTCTTCCTGGATGAAGAAAAAGGCCAGGAGATTGCATTCGTACAGTTCCCACAGAATTTCGAAAATGTTACCAAGAATGAAGTATATAGTAGTACACTACGAATAATCAGTGAGCACAAGAGATGGTCAGAAGGTGATTTCCAGATTTTGGTTTCTAAGTATAGCCCTGCATTGTATGGGCATGGAAAGATTAGTTTAGGCCTTCAACTTGGATATTGCTGCTACTGCCTGTGGGCTCCAAATTGCTTGGCGACACTATTTTACTCAATTCTCCCTTCAATTTACCTTCTTAGAGGCATTTCTGTATTCCCACAGATCTCAAGTCCGTGGTTCATACCATTTGCGTATGTGATAATTGCCAAGTACACTTGGAGCTTTGGGGAGTTTTTGTGGTCTGGTGGCACAATCTTGGGATGGTGGAACGACCAACGAATATGGCTATACAAAAGAACAAGCTCCTACCTATTTGCCTTCATCGATACCATTTTGTACTGTCTAGGGTATTCGGATTCAGCGTTTGTAATAACAGCCAAGGTGGCCGATGAAGATGTGTCACAACGATACGAGAAAGAGATCATGGACTTTGGAGCCTCATCTCCAATGTTCGTCATATTAGCAACAGTTGCATTACTCAATTTGTACTGCTTTGCTGGGTTTCTCAAGGAAGCGATCACTAGAAAAGGCATTGTTGGAGTTTATGAGACAATGGCTCTGCAAATTCTTCTATGTGGTGTTGTAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulose_synt

Protein Analysis

998

Amino Acids

113.19

Weight (kDa)

6.02

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulose_synt PF03552 3 - 198 5e-52 Cellulose synthase
Cellulose_synt PF03552 220 - 510 2.3e-41 Cellulose synthase
Cellulose_synt PF03552 533 - 782 1.7e-77 Cellulose synthase
Cellulose_synt PF03552 791 - 968 3e-16 Cellulose synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55850
fragaria_vesca FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42743 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42750 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42760 FvH4_3g42770 FvH4_3g42800 FvH4_3g42810 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42890 FvH4_3g42900
malus_domestica MD03G1028800.v1.1 MD03G1028900.v1.1 MD03G1029000.v1.1 MD03G1029100.v1.1
prunus_persica Prupe.6G024800_v2.0.a1 Prupe.6G024900_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025000_v2.0.a1 Prupe.6G025100_v2.0.a1 Prupe.6G025100_v2.0.a1
pyrus_communis pycom01g03430 pycom03g02330 pycom03g02340 pycom03g02360 pycom03g02380 pycom03g02400 pycom03g02410 pycom05g13920
rosa_chinensis RchiOBHm_Chr5g0076821 RchiOBHm_Chr5g0076831 RchiOBHm_Chr5g0076851 RchiOBHm_Chr5g0076871 RchiOBHm_Chr5g0076891 RchiOBHm_Chr5g0076901 RchiOBHm_Chr5g0076911 RchiOBHm_Chr5g0076921
rosa_laevigata RLG00000036625 RLG00000036626 RLG00000036627 RLG00000036630 RLG00000036632 RLG00000036634 RLG00000036635
rosa_multiflora Rmu_co8084432.1_g000001 Rmu_co8208886.1_g000001 Rmu_co8325177.1_g000001 Rmu_sc0003799.1_g000001 Rmu_sc0003799.1_g000002 Rmu_sc0003799.1_g000006 Rmu_sc0003799.1_g000009 Rmu_sc0004655.1_g000002 Rmu_sc0004655.1_g000014 Rmu_sc0004655.1_g000015 Rmu_sc0007021.1_g000003 Rmu_sc0007021.1_g000013 Rmu_sc0007021.1_g000014 Rmu_sc0008244.1_g000002 Rmu_sc0013263.1_g000004 Rmu_sc0014402.1_g000001 Rmu_ssc0000184.1_g000003 Rmu_ssc0000184.1_g000004 Rmu_ssc0000184.1_g000005 Rmu_ssc0000184.1_g000006 Rmu_ssc0000184.1_g000014 Rmu_ssc0000184.1_g000021
rosa_roxburghii Rroxscaffold_1G00005210 Rroxscaffold_1G00005220 Rroxscaffold_1G00005250 Rroxscaffold_1G00005280 Rroxscaffold_1G00005300 Rroxscaffold_1G00005310
rosa_rugosa Rorug05G0449200 Rorug05G0449300 Rorug05G0449300 Rorug05G0449300 Rorug05G0449500 Rorug05G0449600
rosa_samantha Rh5AG503600 Rh5AG503700 Rh5AG503900 Rh5AG504000 Rh5AG504100 Rh5BG525000 Rh5BG525200 Rh5BG525300 Rh5BG525500 Rh5BG525700 Rh5BG525900 Rh5BG526000 Rh5CG548600 Rh5CG548800 Rh5CG548900 Rh5CG549100 Rh5CG549300 Rh5CG549500 Rh5DG537300 Rh5DG537400 Rh5DG537500 Rh5DG537700 Rh5DG537900 Rh5DG538000 Rh5DG544100
rosa_wichuraiana Rw0G009920 Rw5G046720 Rw5G046730 Rw5G047200 Rw5G047210 Rw5G047240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 155
AarI CACCTGC 1 cut(s) 1831
Acc16I TGCGCA 1 cut(s) 1535
Acc36I ACCTGC 1 cut(s) 1831
AccB1I GGYRCC 1 cut(s) 959
AccB7I CCANNNNNTGG 2 cut(s) 658, 2401
AccBSI CCGCTC 1 cut(s) 527
AccI GTMKAC 2 cut(s) 216, 2202
AccII CGCG 1 cut(s) 2100
AciI CCGC 6 cut(s) 431, 494, 527, 1423, 1637, 1725
AclWI GGATC 4 cut(s) 44, 1722, 1735, 2036
AcoI YGGCCR 3 cut(s) 403, 622, 2790
AcsI RAATTY 4 cut(s) 1142, 1340, 2308, 2970
AcuI CTGAAG 1 cut(s) 1962
AcvI CACGTG 1 cut(s) 2000
AfiI CCNNNNNNNGG 4 cut(s) 658, 991, 1248, 2401
AflIII ACRYGT 3 cut(s) 219, 339, 1739
AjnI CCWGG 2 cut(s) 2259, 2277
AleI CACNNNNGTG 1 cut(s) 957
Alw21I GWGCWC 2 cut(s) 1616, 2370
Alw26I GTCTC 6 cut(s) 162, 229, 295, 404, 2101, 2948
Alw44I GTGCAC 1 cut(s) 1612
AlwI GGATC 4 cut(s) 44, 1722, 1735, 2036
Ama87I CYCGRG 1 cut(s) 489
ApaLI GTGCAC 1 cut(s) 1612
ApeKI GCWGC 4 cut(s) 1760, 1826, 1932, 2472
ApoI RAATTY 4 cut(s) 1142, 1340, 2308, 2970
ArsI GACNNNNNNTTYG 2 cut(s) 553, 585
AspLEI GCGC 1 cut(s) 1536
AspS9I GGNCC 2 cut(s) 1456, 1546
AsuC2I CCSGG 3 cut(s) 490, 491, 1593
AsuHPI GGTGA 7 cut(s) 1276, 1291, 1411, 1660, 1812, 2111, 2399
AsuII TTCGAA 2 cut(s) 590, 2313
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 1456
BaeGI GKGCMC 2 cut(s) 964, 1616
BaeI ACNNNNGTAYC 2 cut(s) 631, 664
BalI TGGCCA 2 cut(s) 405, 624
BamHI GGATCC 1 cut(s) 1727
BanI GGYRCC 1 cut(s) 959
BanII GRGCYC 2 cut(s) 997, 2491
BauI CACGAG 1 cut(s) 407
BbrPI CACGTG 1 cut(s) 2000
Bbv12I GWGCWC 2 cut(s) 1616, 2370
BbvI GCAGC 4 cut(s) 1747, 1813, 1944, 2459
BceAI ACGGC 3 cut(s) 164, 1632, 1738
BciT130I CCWGG 2 cut(s) 2261, 2279
BciVI GTATCC 2 cut(s) 659, 1776
BclI TGATCA 2 cut(s) 130, 1500
BcnI CCSGG 3 cut(s) 490, 491, 1593
BcoDI GTCTC 6 cut(s) 162, 229, 295, 404, 2101, 2948
BfaI CTAG 5 cut(s) 851, 1094, 1844, 2750, 2928
BfmI CTRYAG 2 cut(s) 1184, 1629
BfuAI ACCTGC 1 cut(s) 1831
BfuI GTATCC 2 cut(s) 659, 1776
BglI GCCNNNNNGGC 1 cut(s) 2789
BglII AGATCT 1 cut(s) 2568
BisI GCNGC 6 cut(s) 528, 1637, 1761, 1827, 1933, 2473
BlpI GCTNAGC 2 cut(s) 1700, 1773
BlsI GCNGC 6 cut(s) 529, 1638, 1762, 1828, 1934, 2474
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 5 cut(s) 490, 491, 1593, 2261, 2279
Bme18I GGWCC 1 cut(s) 1456
BmeT110I CYCGRG 1 cut(s) 489
BmgT120I GGNCC 2 cut(s) 1456, 1546
BmrFI CCNGG 5 cut(s) 490, 491, 1593, 2261, 2279
BmsI GCATC 4 cut(s) 185, 349, 731, 2233
BpmI CTGGAG 2 cut(s) 285, 981
Bpu1102I GCTNAGC 2 cut(s) 1700, 1773
Bpu14I TTCGAA 2 cut(s) 590, 2313
BpuEI CTTGAG 3 cut(s) 1336, 2557, 2897
BpuMI CCSGG 3 cut(s) 490, 491, 1593
Bsa29I ATCGAT 3 cut(s) 39, 684, 2730
BsaAI YACGTR 1 cut(s) 2000
BsaI GGTCTC 2 cut(s) 229, 2101
BsaWI WCCGGW 1 cut(s) 1690
BsaXI ACNNNNNCTCC 4 cut(s) 1802, 1832, 2627, 2657
Bsc4I CCNNNNNNNGG 4 cut(s) 658, 991, 1248, 2401
Bse1I ACTGG 7 cut(s) 406, 507, 550, 769, 998, 1459, 1511
Bse3DI GCAATG 3 cut(s) 313, 1385, 2185
BseBI CCWGG 2 cut(s) 2261, 2279
BseCI ATCGAT 3 cut(s) 39, 684, 2730
BseLI CCNNNNNNNGG 4 cut(s) 658, 991, 1248, 2401
BseMI GCAATG 3 cut(s) 313, 1385, 2185
BseMII CTCAG 5 cut(s) 114, 275, 876, 1714, 1755
BseNI ACTGG 7 cut(s) 406, 507, 550, 769, 998, 1459, 1511
BseRI GAGGAG 1 cut(s) 1026
BseSI GKGCMC 2 cut(s) 964, 1616
BseXI GCAGC 4 cut(s) 1747, 1813, 1944, 2459
BseYI CCCAGC 2 cut(s) 506, 2903
BsgI GTGCAG 1 cut(s) 167
Bsh1236I CGCG 1 cut(s) 2100
BshNI GGYRCC 1 cut(s) 959
BshVI ATCGAT 3 cut(s) 39, 684, 2730
BsiHKAI GWGCWC 2 cut(s) 1616, 2370
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 4 cut(s) 490, 1593, 1691, 2136
BslFI GGGAC 2 cut(s) 741, 1429
BslI CCNNNNNNNGG 4 cut(s) 658, 991, 1248, 2401
BsmAI GTCTC 6 cut(s) 162, 229, 295, 404, 2101, 2948
BsmFI GGGAC 2 cut(s) 741, 1429
BsmI GAATGC 5 cut(s) 280, 1056, 1129, 2152, 2288
Bso31I GGTCTC 2 cut(s) 229, 2101
BsoBI CYCGRG 1 cut(s) 489
Bsp119I TTCGAA 2 cut(s) 590, 2313
Bsp1286I GDGCHC 5 cut(s) 964, 997, 1616, 2370, 2491
Bsp1407I TGTACA 2 cut(s) 1362, 1569
Bsp143I GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
Bsp1720I GCTNAGC 2 cut(s) 1700, 1773
Bsp19I CCATGG 1 cut(s) 1051
Bsp68I TCGCGA 1 cut(s) 2100
BspACI CCGC 6 cut(s) 431, 494, 527, 1423, 1637, 1725
BspCNI CTCAG 5 cut(s) 113, 276, 877, 1713, 1756
BspDI ATCGAT 3 cut(s) 39, 684, 2730
BspFNI CGCG 1 cut(s) 2100
BspMI ACCTGC 1 cut(s) 1831
BspPI GGATC 4 cut(s) 44, 1722, 1735, 2036
BspT104I TTCGAA 2 cut(s) 590, 2313
BspT107I GGYRCC 1 cut(s) 959
BspTNI GGTCTC 2 cut(s) 229, 2101
BsrBI CCGCTC 1 cut(s) 527
BsrDI GCAATG 3 cut(s) 313, 1385, 2185
BsrGI TGTACA 2 cut(s) 1362, 1569
BsrI ACTGG 7 cut(s) 406, 507, 550, 769, 998, 1459, 1511
BssMI GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
BssSI CACGAG 1 cut(s) 407
BssT1I CCWWGG 5 cut(s) 1051, 1071, 1452, 1814, 2784
Bst2BI CACGAG 1 cut(s) 407
Bst2UI CCWGG 2 cut(s) 2261, 2279
Bst6I CTCTTC 4 cut(s) 160, 556, 623, 2099
BstAPI GCANNNNNTGC 3 cut(s) 353, 1124, 2876
BstAUI TGTACA 2 cut(s) 1362, 1569
BstBAI YACGTR 1 cut(s) 2000
BstBI TTCGAA 2 cut(s) 590, 2313
BstC8I GCNNGC 3 cut(s) 478, 1046, 2098
BstDSI CCRYGG 3 cut(s) 180, 1051, 2579
BstEII GGTNACC 1 cut(s) 1818
BstFNI CGCG 1 cut(s) 2100
BstHHI GCGC 1 cut(s) 1536
BstKTI GATC 9 cut(s) 39, 133, 1503, 1730, 1955, 2044, 2571, 2829, 2925
BstMAI GTCTC 6 cut(s) 162, 229, 295, 404, 2101, 2948
BstMBI GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
BstNI CCWGG 2 cut(s) 2261, 2279
BstNSI RCATGY 5 cut(s) 223, 343, 480, 895, 1743
BstPI GGTNACC 1 cut(s) 1818
BstSCI CCNGG 5 cut(s) 488, 489, 1591, 2259, 2277
BstSFI CTRYAG 2 cut(s) 1184, 1629
BstSLI GKGCMC 2 cut(s) 964, 1616
BstUI CGCG 1 cut(s) 2100
BstV1I GCAGC 4 cut(s) 1747, 1813, 1944, 2459
BstX2I RGATCY 3 cut(s) 1727, 2041, 2568
BstYI RGATCY 3 cut(s) 1727, 2041, 2568
Bsu15I ATCGAT 3 cut(s) 39, 684, 2730
BsuI GTATCC 2 cut(s) 659, 1776
BsuTUI ATCGAT 3 cut(s) 39, 684, 2730
BtgI CCRYGG 3 cut(s) 180, 1051, 2579
BtsI GCAGTG 3 cut(s) 278, 1543, 1926
BtsIMutI CAGTG 9 cut(s) 278, 543, 1365, 1518, 1543, 1656, 1926, 2150, 2368
BtuMI TCGCGA 1 cut(s) 2100
BveI ACCTGC 1 cut(s) 1831
Cac8I GCNNGC 3 cut(s) 478, 1046, 2098
CfoI GCGC 1 cut(s) 1536
Cfr13I GGNCC 2 cut(s) 1456, 1546
Cfr9I CCCGGG 1 cut(s) 489
ClaI ATCGAT 3 cut(s) 39, 684, 2730
CspCI CAANNNNNGTGG 4 cut(s) 162, 197, 860, 895
DpnI GATC 9 cut(s) 38, 132, 1502, 1729, 1954, 2043, 2570, 2828, 2924
DpnII GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
EaeI YGGCCR 3 cut(s) 403, 622, 2790
Eam1104I CTCTTC 4 cut(s) 160, 556, 623, 2099
EarI CTCTTC 4 cut(s) 160, 556, 623, 2099
EciI GGCGGA 1 cut(s) 1740
Eco130I CCWWGG 5 cut(s) 1051, 1071, 1452, 1814, 2784
Eco147I AGGCCT 3 cut(s) 820, 1451, 2454
Eco24I GRGCYC 2 cut(s) 997, 2491
Eco31I GGTCTC 2 cut(s) 229, 2101
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 1456
Eco57I CTGAAG 1 cut(s) 1962
Eco72I CACGTG 1 cut(s) 2000
Eco88I CYCGRG 1 cut(s) 489
Eco91I GGTNACC 1 cut(s) 1818
EcoO65I GGTNACC 1 cut(s) 1818
EcoRII CCWGG 2 cut(s) 2259, 2277
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 5 cut(s) 1051, 1071, 1452, 1814, 2784
EcoT22I ATGCAT 2 cut(s) 478, 1129
EcoT38I GRGCYC 2 cut(s) 997, 2491
ErhI CCWWGG 5 cut(s) 1051, 1071, 1452, 1814, 2784
FalI AAGNNNNNCTT 4 cut(s) 938, 970, 1464, 1496
FaqI GGGAC 2 cut(s) 741, 1429
FauI CCCGC 1 cut(s) 1416
FbaI TGATCA 2 cut(s) 130, 1500
FblI GTMKAC 2 cut(s) 216, 2202
Fnu4HI GCNGC 6 cut(s) 528, 1637, 1761, 1827, 1933, 2473
FriOI GRGCYC 2 cut(s) 997, 2491
Fsp4HI GCNGC 6 cut(s) 528, 1637, 1761, 1827, 1933, 2473
FspBI CTAG 5 cut(s) 851, 1094, 1844, 2750, 2928
FspI TGCGCA 1 cut(s) 1535
GlaI GCGC 1 cut(s) 1535
GluI GCNGC 6 cut(s) 528, 1637, 1761, 1827, 1933, 2473
GsaI CCCAGC 2 cut(s) 510, 2907
GsuI CTGGAG 2 cut(s) 285, 981
HapII CCGG 4 cut(s) 490, 1593, 1691, 2136
HhaI GCGC 1 cut(s) 1536
Hin6I GCGC 1 cut(s) 1534
HinP1I GCGC 1 cut(s) 1534
HinfI GANTC 7 cut(s) 41, 82, 288, 665, 1209, 1982, 2762
HpaII CCGG 4 cut(s) 490, 1593, 1691, 2136
HphI GGTGA 7 cut(s) 1276, 1291, 1411, 1660, 1812, 2111, 2399
Hpy188III TCNNGA 5 cut(s) 225, 323, 812, 2099, 2395
Hpy99I CGWCG 1 cut(s) 1209
HpyCH4IV ACGT 2 cut(s) 117, 1999
HpySE526I ACGT 2 cut(s) 117, 1999
HspAI GCGC 1 cut(s) 1534
Ksp22I TGATCA 2 cut(s) 130, 1500
Kzo9I GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
Lsp1109I GCAGC 4 cut(s) 1747, 1813, 1944, 2459
LweI GCATC 4 cut(s) 185, 349, 731, 2233
MaeI CTAG 5 cut(s) 851, 1094, 1844, 2750, 2928
MaeII ACGT 2 cut(s) 117, 1999
MalI GATC 9 cut(s) 38, 132, 1502, 1729, 1954, 2043, 2570, 2828, 2924
MbiI CCGCTC 1 cut(s) 527
MboI GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
MfeI CAATTG 2 cut(s) 141, 1253
MflI RGATCY 3 cut(s) 1727, 2041, 2568
MhlI GDGCHC 5 cut(s) 964, 997, 1616, 2370, 2491
MlsI TGGCCA 2 cut(s) 405, 624
MluNI TGGCCA 2 cut(s) 405, 624
MlyI GAGTC 2 cut(s) 297, 1218
MmeI TCCRAC 3 cut(s) 770, 1228, 2923
Mox20I TGGCCA 2 cut(s) 405, 624
Mph1103I ATGCAT 2 cut(s) 478, 1129
MscI TGGCCA 2 cut(s) 405, 624
MseI TTAA 4 cut(s) 114, 1313, 1878, 2196
MslI CAYNNNNRTG 3 cut(s) 957, 1370, 1607
Msp20I TGGCCA 2 cut(s) 405, 624
MspI CCGG 4 cut(s) 490, 1593, 1691, 2136
MspR9I CCNGG 5 cut(s) 490, 491, 1593, 2261, 2279
MunI CAATTG 2 cut(s) 141, 1253
Mva1269I GAATGC 5 cut(s) 280, 1056, 1129, 2152, 2288
MvaI CCWGG 2 cut(s) 2261, 2279
MvnI CGCG 1 cut(s) 2100
NciI CCSGG 3 cut(s) 490, 491, 1593
NcoI CCATGG 1 cut(s) 1051
NdeII GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
NmuCI GTSAC 7 cut(s) 246, 335, 1210, 1297, 1818, 2000, 2805
NruI TCGCGA 1 cut(s) 2100
NsbI TGCGCA 1 cut(s) 1535
NsiI ATGCAT 2 cut(s) 478, 1129
NspI RCATGY 5 cut(s) 223, 343, 480, 895, 1743
NspV TTCGAA 2 cut(s) 590, 2313
OliI CACNNNNGTG 1 cut(s) 957
PaeI GCATGC 1 cut(s) 480
PaqCI CACCTGC 1 cut(s) 1831
PceI AGGCCT 3 cut(s) 820, 1451, 2454
PciI ACATGT 3 cut(s) 219, 339, 1739
PcsI WCGNNNNNNNCGW 2 cut(s) 1728, 2765
PctI GAATGC 5 cut(s) 280, 1056, 1129, 2152, 2288
PfeI GAWTC 5 cut(s) 41, 82, 665, 1982, 2762
PflMI CCANNNNNTGG 2 cut(s) 658, 2401
PfoI TCCNGGA 1 cut(s) 2259
PkrI GCNGC 6 cut(s) 529, 1638, 1762, 1828, 1934, 2474
PleI GAGTC 2 cut(s) 296, 1217
PmaCI CACGTG 1 cut(s) 2000
PmlI CACGTG 1 cut(s) 2000
PpsI GAGTC 2 cut(s) 296, 1217
Ppu21I YACGTR 1 cut(s) 2000
PscI ACATGT 3 cut(s) 219, 339, 1739
PsiI TTATAA 1 cut(s) 155
Psp6I CCWGG 2 cut(s) 2259, 2277
PspCI CACGTG 1 cut(s) 2000
PspEI GGTNACC 1 cut(s) 1818
PspFI CCCAGC 2 cut(s) 506, 2903
PspGI CCWGG 2 cut(s) 2259, 2277
PspPI GGNCC 2 cut(s) 1456, 1546
PsuI RGATCY 3 cut(s) 1727, 2041, 2568
RruI TCGCGA 1 cut(s) 2100
RseI CAYNNNNRTG 3 cut(s) 957, 1370, 1607
SaqAI TTAA 4 cut(s) 114, 1313, 1878, 2196
SatI GCNGC 6 cut(s) 528, 1637, 1761, 1827, 1933, 2473
Sau3AI GATC 9 cut(s) 36, 130, 1500, 1727, 1952, 2041, 2568, 2826, 2922
Sau96I GGNCC 2 cut(s) 1456, 1546
ScaI AGTACT 1 cut(s) 634
SchI GAGTC 2 cut(s) 297, 1218
ScrFI CCNGG 5 cut(s) 490, 491, 1593, 2261, 2279
SduI GDGCHC 5 cut(s) 964, 997, 1616, 2370, 2491
SfaNI GCATC 4 cut(s) 185, 349, 731, 2233
SfcI CTRYAG 2 cut(s) 1184, 1629
SfuI TTCGAA 2 cut(s) 590, 2313
SinI GGWCC 1 cut(s) 1456
SmaI CCCGGG 1 cut(s) 491
SmiMI CAYNNNNRTG 3 cut(s) 957, 1370, 1607
SmlI CTYRAG 3 cut(s) 1351, 2572, 2912
SmoI CTYRAG 3 cut(s) 1351, 2572, 2912
SphI GCATGC 1 cut(s) 480
SseBI AGGCCT 3 cut(s) 820, 1451, 2454
SsiI CCGC 6 cut(s) 431, 494, 527, 1423, 1637, 1725
SspMI CTAG 5 cut(s) 851, 1094, 1844, 2750, 2928
StuI AGGCCT 3 cut(s) 820, 1451, 2454
StyD4I CCNGG 5 cut(s) 488, 489, 1591, 2259, 2277
StyI CCWWGG 5 cut(s) 1051, 1071, 1452, 1814, 2784
TaiI ACGT 2 cut(s) 120, 2002
TatI WGTACW 9 cut(s) 534, 632, 1362, 1569, 1800, 2345, 2616, 2742, 2892
TauI GCSGC 2 cut(s) 530, 1639
TfiI GAWTC 5 cut(s) 41, 82, 665, 1982, 2762
Tru1I TTAA 4 cut(s) 114, 1313, 1878, 2196
Tru9I TTAA 4 cut(s) 114, 1313, 1878, 2196
TscAI CASTG 9 cut(s) 285, 550, 1372, 1518, 1543, 1663, 1926, 2157, 2368
TseFI GTSAC 7 cut(s) 246, 335, 1210, 1297, 1818, 2000, 2805
TseI GCWGC 4 cut(s) 1760, 1826, 1932, 2472
Tsp45I GTSAC 7 cut(s) 246, 335, 1210, 1297, 1818, 2000, 2805
TspGWI ACGGA 1 cut(s) 2568
TspMI CCCGGG 1 cut(s) 489
TspRI CASTG 9 cut(s) 285, 550, 1372, 1518, 1543, 1663, 1926, 2157, 2368
Van91I CCANNNNNTGG 2 cut(s) 658, 2401
VneI GTGCAC 1 cut(s) 1612
VpaK11BI GGWCC 1 cut(s) 1456
XapI RAATTY 4 cut(s) 1142, 1340, 2308, 2970
XceI RCATGY 5 cut(s) 223, 343, 480, 895, 1743
XmaI CCCGGG 1 cut(s) 489
XmiI GTMKAC 2 cut(s) 216, 2202
XspI CTAG 5 cut(s) 851, 1094, 1844, 2750, 2928
ZrmI AGTACT 1 cut(s) 634
Zsp2I ATGCAT 2 cut(s) 478, 1129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.